KR20190014435A - Method for producing buckwheat sprout with increased rutin contents - Google Patents

Abstract

The present invention relates to germinated Fagopyrum esculentum with increased rutin content, germinated under a specific germination condition to increase the rutin content; to a method for producing an ethanol extract of germinated Fagopyrum esculentum with the increased rutin content; and to a functionality thereof. The method for producing the germinated Fagopyrum esculentum of the present invention significantly improves the rutin content in germinated Fagopyrum esculentum. Since the rutin content was significantly increased in an extract obtained by extracting sprouts of the germinated Fagopyrum esculentum produced by the method under a specific condition, the extract can be used as a high-value agricultural product. Also, since the extract has excellent antioxidant and whitening activity, the extract can be utilized as a functional cosmetic product or a functional food material.

Description

METHOD FOR PRODUCING BUCKWHEAT SPROUT WITH INCREASED RUTIN CONTENTS BACKGROUND OF THE INVENTION [0001]

More particularly, the present invention relates to a method for improving the rutin content of buckwheat, and more particularly, to a method for improving the rutin content of buckwheat, Methods and their functionality.

Because modern women prefer white, clean skin, there is growing interest in the treatment of skin pigmentation disorders and the need for skin care to meet beauty needs. Skin is a very important tissue that protects the human body in direct contact with the external environment and has biochemical and physical functions. Melanin is a pigment present in the epidermis of the skin. It is made from melanocytes in the basal layer of the epidermis and migrates to epidermal cells called keratinocytes. In vivo, there is no enzymes that degrade melanin, but keratinocytes are removed from the skin as they fall off the epidermis. Tyrosine is converted to DOPAquinone via DOPA by the tyrosinase enzyme in the melanocytoin in the melanocyte site and is converted to DOPAquinone by the DopAchrome through the autoxidation reaction and the enzyme reaction of dopaquinone, Melanin is produced. The resulting melanin is transferred to the keratinocyte through the melanoma, and after about 28 days of keratinization, it exits the skin surface and disappears with the vagina. Melanin absorbs the light energy of sunlight UV and protects the subcutaneous skin organs from UV damage. It also helps protect the skin from external harmful factors such as harmful oxygen and free radicals generated in the skin. Role. In addition, melanin in skin cells determines the skin color of a person. People with high melanin pigment have brown or black skin while those with low melanin pigment have white skin. As a whitening method for skin cosmetics, there is known a method of decolorizing by reducing the produced melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. The whitening agent using tocopherol, vitamins or the like for reducing the melanin pigment has a small discoloring effect of the melanin pigment, so that an inhibitor that inhibits the activity of the tyrosinase has been attracting attention.

On the other hand, buckwheat is a dicotyledonous plant belonging to the family Polygonaceae. The buckwheat variety commonly known as perennial is called common buckwheat ( Fagopyrum esculentum Moench ) and tartary buckwheat ( Fagopyrum tataricum Gaertner) There is tatami buckwheat. The buckwheat contains 12 to 15% protein, especially contains a large amount of essential amino acids, and contains a relatively large amount of minerals such as fat, iron, phosphorus, copper, zinc and vitamins B1 and B2, Is known to have a very high content of routinely used drugs. The content of these routines was much higher in the tartar buckwheat than in the general buckwheat, but it was higher when germinated, but the routine content could not be dramatically increased in general germination. Rutin (quercetin-3-rutinoside) is a flavonoid substance which is widely distributed in nature. It has higher content in buckwheat than other grains, and buckwheat is the origin of Rutin because it was first separated from buckwheat. Rutin is a combination of quercetin with rutinose (disaccharide with rhamnose and glucose), which is yellow to light yellow. It is known that a routine known as vitamin P is an important ingredient for preventing the intracerebral hemorrhage by strengthening resistance of blood vessels and also has a pharmacological effect for inhibiting excessive permeability of blood vessels as a therapeutic agent for vascular diseases. Recently, it has been shown that diabetes and cancer are also effective, and research on it has become more active.

In order to produce germinated buckwheat with improved rutin content, the present inventors have found that optimum germination conditions capable of dramatically increasing the content of rutin by changing moisture, light, and oxygen, which are germination conditions, Buckwheat sprouts were used as agricultural high value crops and extracted from them to utilize them as food and functional foods.

It is an object of the present invention to provide a method for producing germinated buckwheat with increased rutin content.

It is another object of the present invention to provide a buckwheat sprout having an increased rutin content.

It is another object of the present invention to provide a method for producing an ethanol extract of buckwheat sprouts with increased rutin content.

It is another object of the present invention to provide an antioxidative and whitening composition containing an extract of buckwheat ethanol as an active ingredient.

In order to achieve the above object, the inventors of the present invention derive optimal germination conditions of tartar buckwheat to maximize the rutin content of buckwheat, and have extracted extraction conditions containing the rutin content significantly in the extract of germinated buckwheat sprouts, The functionality of the extract was confirmed.

The germinated buckwheat production method of the present invention remarkably improves the germinated buckwheat rutin content, and the germinated buckwheat sprouts produced by the above method have been remarkably increased in the extracts obtained by extracting the germinated buckwheat seeds under specific conditions. Therefore, the germinated buckwheat can be used as a high value added agricultural product Since the extract has excellent antioxidative and whitening activity, it can be utilized as a functional cosmetic or a functional food material.

Fig. 1 is a diagram illustrating the germination process of buckwheat.
FIG. 2 is a graph showing the content of rutin in the extract of buckwheat sprout or ethanol according to the germination condition.
FIG. 3 shows antioxidant activity of the extract of buckwheat hot water or ethanol according to germination conditions.
FIG. 4 is a view showing tyrosinase-inhibiting activity of the extract of buckwheat hot water or ethanol according to the germination condition.

Hereinafter, the present invention will be described in detail with reference to embodiments of the present invention. It should be understood, however, that the invention is not limited thereto and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents. .

In one aspect, the present invention provides a method of making buckwheat, comprising: dipping buckwheat in water; Growing under the dark condition at 18 to 22 캜 for 20 to 30 hours; And germinating the buckwheat for 8 days under the light condition of 14,000 Lux or more, to produce germinated buckwheat with increased rutin content.

In one embodiment, the light condition may be an LED.

In one embodiment, the buckwheat may be a Tartary Buckwheat.

In one aspect, the invention relates to buckwheat sprouts having increased rutin content prepared by the method of the present invention.

In one aspect, the present invention provides a method of making buckwheat, comprising: dipping buckwheat in water; Growing under the dark condition at 18 to 22 캜 for 20 to 30 hours; Growing the buckwheat by growing for 8 days under a light condition of 14,000 Lux or more; Drying and pulverizing the germinated buckwheat; And extracting the germinated buckwheat powder with ethanol. The present invention also relates to a method for producing an ethanol extract of buckwheat sprouts having an increased rutin content.

In one embodiment, extraction with ethanol followed by sonication may be further included.

In one embodiment, the buckwheat germination conditions may be 17-20 < 0 > C, relative humidity 80-100%.

As used herein, the term "extract" means an active ingredient isolated from a natural product, i.e., a substance exhibiting the desired activity. The extract can be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes all the forms that are formulated using the dry powder of the extract. In addition, the above-mentioned extract also includes fractions obtained by fractionating the extract obtained through the above extraction process.

The extraction solvent of the extract of buckwheat sprouts of the present invention may be a polar solvent such as water, a C ₁ -C ₄ lower alcohol, a nonpolar solvent such as hexane, chloroform, dichloromethane or ethyl acetate, or a mixture of two or more thereof However, since the ethanol content of the ethanol extract is remarkable, the ethanol extract extracted with ethanol is most preferable.

In one embodiment, the rutin content of the buckwheat ethanol extract produced by the process of the present invention may be greater than 1000 ppm.

In one aspect, the present invention relates to an antioxidative composition comprising an extract of buckwheat ethanol produced by the method of the present invention as an active ingredient.

In one aspect, the present invention relates to an antioxidant food composition comprising an extract of buckwheat ethanol produced by the method of the present invention as an active ingredient.

The food composition may be provided in the form of a powder, granules, tablets, capsules, syrups or beverages. The food composition may be used with food or food additives other than the active ingredient HSF1 inhibitor and BIS inhibitor according to the present invention, Can be suitably used according to the method of The amount of the active ingredient to be mixed can be suitably determined according to the intended use, for example, prevention, health or therapeutic treatment.

There is no particular limitation on the kind of the health food, and examples thereof include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, Drinks, alcoholic beverages and vitamin complexes.

In one aspect, the present invention relates to a whitening composition comprising an extract of buckwheat ethanol produced by the method of the present invention as an active ingredient.

In one aspect, the present invention relates to a cosmetic composition for whitening and antioxidation comprising an extract of buckwheat ethanol produced by the method of the present invention as an active ingredient.

The cosmetic composition of the present invention can be prepared by incorporating a cosmetically effective amount of the oligosaccharide as an effective ingredient of the present invention and an cosmetically acceptable carrier.

As used herein, the term " cosmetically effective amount " means an amount sufficient to achieve the antimicrobial or anti-inflammatory efficacy against the acne-causing bacteria of the composition of the present invention described above.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredient and carrier component, ingredients conventionally used in the composition for external application for skin, and they can be used in a conventional manner such as an antioxidant, a stabilizer, a solubilizer, a vitamin, May include adjuvants.

The present invention will be described in more detail with reference to the following examples. However, the following examples are only for the purpose of illustrating the present invention, and thus the present invention is not limited thereto.

Example . Establishment of optimal germination condition

The tatami buckwheat was washed and then immersed in the same volume of water at 20 ° C for 48 hours. Thereafter, germination was carried out under a dark condition at 20 DEG C for 24 hours and under a light condition (LED) at 20 DEG C for 8 days. Specifically, light conditions were germinated at light intensity of 2,000, 6,000, 10,000, 14,000, 18,000 and 22,000 Lux, respectively. The germinated tartar buckwheat was dried and powdered. The hot water or ethanol was added in a volume of 20 times the weight of the germinated tartar buckwheat, and then extracted at 2.5 Hz for 30 minutes using an ultrasonic extractor. (Fig. 1).

Water extract (ppm) Ethanol extract (ppm) 10000Lux 70.24 581.23 14000Lux 79.91 1059.57 18000Lux 70.84 1110.78 22000Lux 85.47 1225.85

As a result, the germinated hot water extract of germinated tartaric acid showed the contents of 70.24, 79.91, 70.84 and 85.47ppm according to the intensity of light (10000, 14000, 18000, 22000 Lux), and the germinated tartaric acid ethanol extract showed 581.23, 1059.57, 1110.78 , And 1225.85 ppm, respectively. In the case of extracting with ethanol, the content of rutin was remarkably increased at 14000 Lux (Table 1 and Fig. 2).

Experimental Example  1. Germination Tatari  Of buckwheat extract Antioxidant ability  Confirm

The radical scavenging activity of germinated hydrolyzate or ethanol extracts was determined by measuring the ABTS ⁺ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity using ABTS ⁺ decolorization assay. Specifically, ABTS7 mM and 2.5 mM potassium persulphate were left in the dark for one day, and the solution was diluted with distilled water to an absorbance value of 1.4 to 1.5 at 734 nm. Dilution to 160 μl of diluted ABTS ⁺ Ascorbic acid was used as a standard substance and the concentration (IC ₅₀ ) of the sample when the activity inhibition rate was 50% was calculated by the following equation 1.

a: Absorbance after reaction of blank sample

b: Absorbance after reaction of the sample solution

b ': absorbance measured by replacing with buffer

Type of extract Mark Extract of hot tapiata grated from 10,000Lux H2O 1 Extracted from 14,000Lux of hot water extract of tartar buckwheat H2O2 Extracted from the 18,000Lux tartar tamarin hot water extract H2O 3 Ethanol Extract of Tartary Buckwheat Germinated from 10,000ux EtOH 1 Ethanol extract of tartaric acid in germinated at 14,000ux EtOH 2 Tartaric acid ethanol extract germinated at 18,000ux EtOH 3

As a result of comparing ABTS ⁺ radical scavenging activity of germinated ethanol or hot water extract of germination according to the intensity of light, the ethanol content increased with increasing germination intensity, (Table 3 and Fig. 3). ≪ tb >< TABLE > This suggests that the ABTS ⁺ radical scavenging ability is dependent on the rutin content of ethanol and hot water extract.

ABTS + radical scavenging activity ± error NC 0.54 1.30 EtOH 1 62.48 0.85 EtOH 2 69.49 0.48 EtOH 3 77.21 1.47 H2O 1 23.34 0.65 H2O2 27.17 1.25 H2O 3 23.21 1.12 1L-ascorbic acid 0.0281 (μg / ml) 0.0008 (μg / ml)

¹ as a positive control of ABTS ⁺ radical scavenging activity was expressed by IC ₅₀ values.

(n = 3; ****: P <0.0001 vs non-treated group).

Experimental Example  2. Germination Tatari  Confirm whitening function of buckwheat extract

The tyrosinase inhibitory activity of the germinated hydrolyzate or ethanol extract of germinated tartaric acid was measured. Specifically, 140 μl of 0.1 M phosphate buffer (pH 6.5), 20 μl of an extract sample, and 20 μl of tyrosinase (1500-2000 U / ml) were sequentially added to a 96-well plate and then 1.5 mM tyrosinase 20 And then reacted at 37 ° C for 20 minutes. The absorbance was then measured at 475 nm using a microplate reader. As a negative control, 0.1 M phosphate buffer (pH 6.5) was used instead of the sample solution (extract), and Arbutin was used as a positive control. The concentration of the sample (IC ₅₀ ) at the 50% active inhibition rate was calculated using absorbance measurements.

Tyrosinase inhibitory activity ± error NC 0.54 1.30 EtOH 1 62.48 0.85 EtOH 2 69.49 0.48 EtOH 3 77.21 1.47 H2O 1 23.34 0.65 H2O2 27.17 1.25 H2O 3 23.21 1.12 Arbutin 0.18 (mM) 0.03 (mM)

¹ tyrosinase inhibitory activity as an IC ₅₀ value.

(n = 3; ****: P <0.0001, *: <0.05 vs. non-treated group).

The tyrosinase inhibitory activity of germination ethanol or hot-water extract of germinated ginseng according to the intensity of light (Lux) was compared with that of tyrosinase inhibitory activity (Fig. 4).

Claims (7)

1) immersing buckwheat in water;
2) growing under the dark condition of 18 to 22 캜 for 20 to 30 hours; And
3) Growing the buckwheat by growing for 8 days under a light condition of 14,000 Lux or more, thereby producing germinated buckwheat with increased rutin content. The method of claim 1, wherein the buckwheat is tartaric buckwheat. A buckwheat sprout having increased rutin content prepared by the method of claim 1. 1) immersing buckwheat in water;
2) growing under the dark condition of 18 to 22 캜 for 20 to 30 hours;
3) germinating buckwheat grown under light condition of 14,000 Lux or more for 8 days;
4) drying and pulverizing the germinated buckwheat; And
5) Extracting the germinated buckwheat powder with ethanol. A method for producing an ethanol extract of buckwheat sprouts having increased rutin content. 5. The method according to claim 4, wherein the rutin content of the buckwheat sprout ethanol extract is 1000 ppm or more. An antioxidant and whitening cosmetic composition comprising an extract of buckwheat ethanol produced by the method of claim 4 as an active ingredient. An antioxidant food composition comprising an extract of buckwheat ethanol produced by the method of claim 4 as an active ingredient.

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