JPH05124950A - Beautifying and whitening cosmetic composition - Google Patents

Abstract

PURPOSE:To obtain the subject safe composition, containing an extract of Aquillariae Lignum capable of remarkably suppressing melanogenesis without exhibiting cytotoxicity as an active ingredient, having high beautifying and whitening effects and useful for preventing or treating dermal stain, ephelides, etc. CONSTITUTION:The objective composition is obtained by blending an extract of Aquillariae Lignum as an active ingredient so as to provide preferably 2-20wt.% expressed in terms of the raw material. The extract of the Aquillariae Lignum is preferably prepared by a method for finely cutting a woody part of Aquillaria agallocha Roxb. or Aquillaria sinensis, then extracting the finely cut woody part with a solvent composed of water and ethanol in a volume of 10-20ml based on 1g finely cut raw material and dividedly carrying out repeated extraction of the raw material 2-4 times at ambient temperature for 2-5 days.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition having an excellent whitening effect, and more specifically, it is particularly effective in preventing or treating spots, freckles and the like, and has an excellent whitening effect. The present invention relates to a whitening cosmetic composition having both stability and high safety to the skin.

[0002]

2. Description of the Related Art Conventionally, hydroquinone and its glycoside, for the purpose of preventing or treating spots, freckles, etc.,
There has been proposed a whitening cosmetic composition containing substances such as kojic acid and its derivatives, ascorbic acid and its derivatives, thiol compounds, and various animal and plant extracts as a whitening component.

[0003]

However, various problems remain in these cosmetics, and there is a problem in terms of practicality. For example, hydroquinone as a whitening component has a problem in safety, and hydroquinone glycosides, kojic acid and its derivatives, ascorbic acid and its derivatives, etc. have a high polarity for use as a blending component of cosmetics. There is a problem that it is too much.

Further, thiol compounds such as glutathione and cysteine still have problems in stability after compounding, and other animal and plant extracts such as placenta extract,
Aloe extract and other ingredients are known, but their whitening effect was not satisfactory.

[0005]

[Means for Solving the Problems] In order to find a substance having an excellent whitening effect, the present inventors screened various plant extracts using a melanin production inhibition test using a mouse B16 melanoma strain as an index. went. As a result, as a result, gingko (Aq
uillaria agallocha) and incense Shiraki (Aquillaria sinen)
It was found that an extract of the woody part of sis) (hereinafter referred to as "agarwood extract") strongly suppresses melanin production and has extremely weak cytotoxicity, and is extremely effective as a whitening ingredient of cosmetics.

The present invention has been completed based on the above findings, and provides a composition for whitening cosmetics containing an agarwood extract as an active ingredient.

In the present invention, the whitening cosmetic composition means
A skin cosmetic composition effective for the prevention or treatment of spots, freckles, etc., a lotion as a quasi drug, an emulsion,
It also includes cosmeceuticals such as creams, packs and soaps, and lotions as pharmaceuticals, and external preparations for skin such as emulsions, creams and ointments.

The agarwood extract used in the present invention is obtained by extracting the woody part of Jinko or Shirako scent with a solvent. Jinkou and Shiraki incense have long been known for their resinized woody parts as perfumes, and they are used for asthma, vomiting, abdominal pain, and coldness in the lower back and knees, with the expectation of a kind of sedative action and fatigue recovery action for medicinal purposes. Has been. It has also been used as an insect repellent since ancient times. However, it is completely unknown that this is effective as a whitening agent.

The solvent used to obtain the agarwood extract is not particularly limited as long as it is a solvent in which the active ingredient is effectively extracted, but water or acetone, methanol, ethanol, propanol and the like can be used. It is preferable to use an organic solvent that is miscible with water, such as a lower alcohol, or a mixed solution thereof, and it is more preferable to use water or ethanol or a mixed solution thereof.

The agarwood extract can be obtained, for example, by directly extracting the agarwood, which is available at the woody part of Jinko, Shiraki incense, Chinese herb pharmacy, etc., with a solvent, but preferably in order to enhance the extraction efficiency, It is desirable that these raw materials be finely cut and then extracted with a solvent. The extraction is 5 to 100 ml, preferably 10 per 1 g of the finely cut raw material.
It is preferable to use ~ 20 ml of a solvent for 1 to 1 month, preferably 2 to 5 days, and repeat 2 to 4 times at room temperature. Further, in order to increase the concentration of the active ingredient in the agarwood extract, the agarwood extract obtained may be further added, if desired.
It is also possible to apply means such as concentration, liquid-liquid distribution, adsorption chromatography, normal phase or reverse phase chromatography and the like.

The whitening cosmetic composition of the present invention comprises the agarwood extract obtained as described above and the components generally used in cosmetics, such as oily components, surfactants, ultraviolet absorbers and lower alcohols. , Preservatives, germicides, colorants, powders,
Match the dosage form with fragrance, water-soluble polymer, buffer, etc.
It is prepared by appropriately mixing the components within a range that does not impair the effects of the present invention.

The content of the agarwood extract in the whitening cosmetic composition of the present invention is preferably determined in consideration of the melanin production inhibitory effect and the skin keratin permeability. Generally, the agarwood extract is converted into the raw material. It should be 0.1 to 50% by weight, preferably 2 to 20% by weight.

[0012]

It is not yet known how the agarwood extract, which is the active ingredient of the present invention, acts to exhibit a whitening effect.
However, in the melanin production inhibition test using the B16 melanoma strain of the mouse, the agarwood extract strongly inhibits melanin production without showing cytotoxicity, and this reaction was observed at any step from tyrosine to melanin production. It is expected to inhibit the

[0013]

The present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

Example 1 Preparation of agarwood extract: 2 g of perfumed agarwood (obtained from Kampo medicine dealer) was finely cut, 30 ml of 50% ethanol was added, and the mixture was extracted at room temperature for 3 days. After the extract was filtered off, the residue was reextracted twice in the same manner, and the obtained extract was combined with the previous extract. Concentrate it to dryness,
176 mg of crude extract was obtained.

Example 2 Melanin production inhibition test: The melanin production inhibition test was carried out as follows using mouse melanoma cells. First, 1
× 10 ⁵ B16 melanoma cells were seeded in a petri dish having a diameter of 60 mm containing 5 ml of Eagle's minimal nutrient medium containing 10% (v / v) fetal bovine serum and adjusted to 5% (v / v) carbon dioxide. The cells were cultured in a carbon dioxide gas incubator at 37 ° C for 24 hours. Next, 50 μl of a sample dissolved in pure water or 20% ethanol was added to this petri dish. After further culturing for 5 days under the same conditions, cells were collected by trypsin treatment, the degree of whitening was visually evaluated, and at the same time, the change in cell mass volume was visually evaluated and used as an index of cytotoxicity.

Using this measurement method, the agarwood extract obtained in Example 1 was measured for melanin production inhibition at various concentrations. The results are shown in Table 1. For comparison, arbutin (S. Akiu et al., Whose whitening effect is known,
Proc. Jpn. Soc. Invest. Dermatol., 12 Vol. 138 (198
8)) was also measured in the same manner.

[0017] * Whiteness judgment criteria: Judgment content ++ High whiteness. + Moderate whiteness. ± slightly whitened. -No whitening.

As is clear from Table 1, the agarwood extract showed a melanin production inhibitory activity which was 5 times or more that of arbutin. In addition, arbutin showed a decrease in cell mass volume when the sample was added at 20 μg / ml, and cells were killed when the sample was added at 50 μg / ml, whereas the agarwood extract was 50 μg.
It was found that the cytotoxicity was extremely weak, with no change in the volume of the cell mass observed even when the sample was added in the amount of / ml.

Example 3 Production of whitening cream: [Method] A Extract of Example 1 0.01 g Purified water 5.54 g B 3-succinyloxyglycyl retinoic acid disodium sodium 0.05 g C squalane 10 0.000 g Octyldodecyl myristate 8.00 g Microcrystalline wax 4.00 g Behenyl alcohol 3.00 g Lipophilic glyceryl monostearate 2.50 g Polyoxyethylene sorbitan monostearate (20 E.O.) 2.50 g D 1,3-butylene Glycol 10.00 g Methyl paraoxybenzoate 0.10 g Purified water 54.00 g E Fragrance 0.30 g

[Production Method] B was added to D heated to 80 to 85 ° C., and C which was heated and dissolved at 80 to 85 ° C. was added thereto with stirring with a homomixer to uniformly emulsify. It was gradually cooled to about 50 ° C. at room temperature and E and suspended A were added. The whitening cream was manufactured by further cooling to room temperature while continuing stirring. The whitening cream thus produced had an excellent whitening effect.

Example 4 Production of Carmine Lotion: [Method] A zinc oxide 1.30 g silicic acid anhydride 1.10 g tar 2.00 g bengala 0.01 g polyoxyethylene stearic acid amide (4E. O.) 0.05 g B Extract of Example 1 0.01 g Ethanol 5.59 g Purified water 5.00 g C Concentrated glycerin 3.00 g Caffle 0.10 g Methyl paraoxybenzoate 0.05 g Fragrance 0.1 05g D Purified water 81.74g

[Production Method] About 60 g of D was added to A and uniformly dispersed with a homomixer to prepare a powder dispersion.
A solution of B and C were added to this, the rest of D was added, and the mixture was uniformly dispersed with a homomixer to produce a carmine lotion. The carmine lotion produced in this manner had a hot whitening effect and an excellent whitening effect.

Example 5 Production of whitening ointment: [Method] A Macrogol 4000 47.50 g Macrogol 400 47.50 g B Extract from Example 1 0.03 g Purified water 4.97 g

[Manufacturing Method] Macrogol 4000 and Macrogol 400 were heated to 65 ° C. in a water bath to dissolve, and uniformly mixed to manufacture a Macrogol ointment base.
The solution of B was kneaded with this to produce a whitening ointment. The whitening ointment thus produced was excellent in whitening effect.

[0025]

The agarwood extract of the present invention remarkably suppresses melanin production without showing cytotoxicity. Therefore, the cosmetic composition for whitening containing the agar extract or the purified product of the present invention is safe and has a high whitening effect.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Junsei Asami 1-1-1, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka Suntory Ltd.

Claims (1)

[Claims] 1. A whitening cosmetic composition comprising an agarwood extract as an active ingredient.