JPH0930948A - Beautifying and whitening dermal preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a beautifying and whitening dermal preparation for external use, having suppressing actions on melanogenesis and inhibiting actions on tyrosinase activities and effective in preventing and reducing pigmentation, dermal stains, ephelides, chloasma, etc., after the sunburn. SOLUTION: This beautifying and whitening dermal preparation for external use contains 0.005-20wt.%, preferably 0.01-10wt.% extract of a plant, growing in especially a dry grassland, a stock farm, etc., of Indonesia and belonging to the genus Kaempferia of the family Zingiberaceae, preferably Kunei pepet (botanical name: Kaempferia angustifolia) as an essential ingredient therein. The extract, as necessary, is suitably further blended with a beautifying and whitening agent, a humectant, an antioxidant, an oily ingredient, an ultraviolet absorber, a surfactant, a thickening agent, alcohols, a powdery ingredient, a colorant, an aqueous ingredient, water, various dermal nutrients, etc., and prepared into an ointment, a cream, a milky lotion, a lotion, a pack, a bathing agent, etc. Furthermore, the extract is obtained by immersing or refluxing a leaf, a stem, a fruit, etc., of the plant, the whole herb, etc., thereof together with an extracting solvent (e.g. ethanol) under heating, then filtering the resultant extract and concentrating the prepared filtrate.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to ginger (Zingiberacea)
e) By adding an extract of the family Kaempferia, the melanin production is suppressed, and it is effective for preventing and improving pigmentation, stains, freckles, and liver spots after sunburn. Regarding agents.

[0002]

2. Description of the Related Art Although there are some unclear points about the mechanism of the occurrence of skin spots and the like, melanin pigments are generally formed due to hormonal abnormalities and stimulation of ultraviolet rays from sunlight. It is believed to be abnormally deposited within. This melanin pigment, which causes skin coloring, is produced in melanin-producing granules (melanosomes) in melanocytes (melanocytes) between the epidermis and dermis,
The generated melanin diffuses into adjacent cells by the osmotic effect. The biochemical reaction in this melanocyte is presumed to be as follows. That is, tyrosine, which is an essential amino acid, is converted into dopaquinone by the action of the enzyme tyrosinase, and the process in which it is converted to black melanin via a red pigment and a colorless pigment by an enzymatic or non-enzymatic oxidative action is a melanin pigment production process. Therefore, suppressing the action of tyrosinase, which is the first step of the reaction, is important for suppressing melanin production.

[0003]

However, compounds that suppress the tyrosinase action, except for hydroquinone, have very slow onset of their effects, so that the effect of improving skin pigmentation is not sufficient. On the other hand, although hydroquinone is recognized as having an effect, its use is generally restricted because of its sensitizing properties. Therefore, in order to improve its safety, attempts have been made to use higher fatty acid monoesters or alkyl monoethers (JP-A-58-154507), but the esters are decomposed by a hydrolase in the body. Therefore, it is not always safe, and ethers have not been sufficiently satisfactory in terms of safety.

[0004]

Therefore, as a result of investigating the melanin production inhibitory effect of various substances as a solution to these problems, the present inventors have found that ginger (Zingibe
The present inventors have found that an extract of a plant of the genus Kaempferia of the family Raceae has a melanin production inhibitory action and a tyrosinase inhibitory action, and completed the present invention. Ginger (Zingiberaceae) Family Kempferia (Kaem
There have been no reports of melanin production inhibitory effects of extracts of plants of the genus pferia), and their application to whitening agents is not known. The present inventors have completed the present invention based on the above findings.

That is, the present invention relates to ginger (Zingiberace)
ae) An external preparation for whitening skin, characterized by containing an extract of a plant of the genus Kaempferia. More specifically, Kunei Pepe
t, scientific name: Kaempferia angustifolia).

The structure of the present invention will be described in detail below. As a plant of the genus Kempferia (Kaempferia) of the ginger (Zingiberaceae) family used in the present invention, there is Kunei Pepeto.
(Kunei pepper, scientific name: Kaempferia angusti
folia) is preferred. This is a plant that grows especially on dry grasslands, pastures and the like in Indonesia. Extracts used in the present invention, the leaves of the above plants, stems including rhizomes, fruits, etc.
After immersing the whole plant with the extraction solvent or heating to reflux,
Obtained by filtration and concentration. The extraction solvent used in the present invention may be any solvent used in ordinary extraction,
In particular, alcohols such as methanol and ethanol, hydroalcohols, organic solvents such as acetone and ethyl acetate can be used alone or in combination.

Ginger (Zingiberaceae) in the present invention
The amount of the extract of the plant of the genus Kaempferia belonging to the family Kaempferia is 0.005 to 20% as a dry product in the total amount of the external preparation.
It is 0% by weight, preferably 0.01 to 10.0% by weight. If the amount is less than 0.005% by weight, the effects of the present invention are not sufficiently exhibited, and if the amount is more than 20.0% by weight, it is not preferable because formulation is difficult. Further, even if the content is 10.0% by weight or more, the effect is not so much improved.

In addition to the above-mentioned essential components, the external whitening agent for skin whitening of the present invention also contains components that are usually used in external skin externalizing agents such as cosmetics and pharmaceuticals, such as other whitening agents and moisturizers.
Antioxidants, oily components, UV absorbers, surfactants, thickeners, alcohols, powder components, coloring materials, aqueous components, water,
Various skin nutrients and the like can be appropriately compounded as needed.

In addition, sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice extraction Substance, glabridin, hot water extract of fruits of fire thorns, various crude drugs, tocopherol acetate,
Drugs such as glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate,
Other whitening agents such as ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, mannose,
Sugars such as sucrose and trehalose can also be appropriately blended.

The external whitening agent for whitening of the present invention includes, for example, ointments, creams, emulsions, lotions, packs, bath agents and the like.
Any conventional skin external preparation may be used,
The dosage form is not particularly limited.

[0011]

Next, the present invention will be described in more detail by way of examples. Note that the present invention is not limited by this. The blending amount is% by weight. Prior to Examples, test methods and results of the melanin suppressing effect, tyrosinase activity inhibiting effect and whitening effect of the plant extract of the present invention will be described.

Test Method and Results 1. Sample Preparation (1) Kunei Pepet, Scientific name:
Kaempferia angustifolia) extract 50 whole plants of Kunei pepper
g was immersed in methanol for 1 week at room temperature and the extract was concentrated to obtain 0.91 g of a methanol extract. This extract was dissolved in DMSO at 1%, the concentration was adjusted by diluting this solution, and the following experiment was performed using this.

2. Cell culture method B16 melanoma cultured cells derived from mice were used. 1
0% FBS and theophylline (0.09 mg / ml)
The cells were cultured in an Eagle MEM medium containing C.sub.2 in a CO.sub.2 incubator (95% air, 5% carbon dioxide) at 37.degree. After 24 hours of culturing, the sample solution was added so that the final concentration (concentration of extracted dry matter) was 10 −2 to 10 −5% by weight, and the culturing was continued for another 3 days. The determination and the tyrosinase activity inhibitory effect were measured.

3. Visual perception of melanin amount Place a diffusion plate on the lid of the well plate, observe the intracellular melanin amount with an inverted microscope, and use Kunei Pete (Ku
(nei-pepet, scientific name: Kaempferia angustifolia)
It was compared with the case of the sample (reference) to which the extract of 1. was not added. The results are shown in Table 1. Also, as a reference example,
The same test as described above was carried out also on an extract of mussel (Lamiaceae, Odorikosou subfamily), which is already known to have a melanin production inhibitory action. Table 1 also shows the results. Further, in the table, "toxic" means that it is cytotoxic.

<Judgment Criteria> ◯: White (amount of melanin) Δ: Slightly white (amount of melanin) ×: Reference (amount of melanin)

[0016]

[Table 1]

Test Melanin production Visual evaluation Concentration (% by weight) 10-5 10-4 10-3 10-2 Kunei Hauphet extract △ △ ○ Toxicity Kaigai extract × × × ×

5. Whitening test [Test method] 40 subjects exposed to sunlight in summer for 4 hours (2 hours per day for 2 days) each sample from 5 days after the day of sun exposure to the skin of the upper arm inner skin Was applied once each morning and evening for 4 weeks. The panel was divided into 8 groups, and 5 groups were prepared, and the tests were conducted according to the formulations shown below. (Alcohol phase) 95% Ethyl alcohol 55.0% by weight Polyoxyethylene (25 mol) hydrogenated castor oil ether 2.0 Antioxidant / preservative proper amount Fragrance proper amount drug (listed in Table 2) (water phase) glycerin 5.0 Sodium hexametaphosphate Suitable amount Ion-exchanged water Residue <Production method> An aqueous phase and an alcohol phase are prepared, respectively, and then both are mixed and solubilized.

[Evaluation Method] The lightening effect after use was judged based on the following judgment criteria. <Judgment criteria> ：: When the ratio showing significant effect and effectiveness among the subjects is 80% or more 割 合: The ratio showing significant effect and effectiveness among the subjects is 50% or more
Less than 80% △: The proportion of the test subjects showing excellent and effective is 30% or more
When less than 50% x: When the ratio of markedly effective or effective among the subjects is less than 30%

Samples having the blending composition described in the above test method were prepared and the whitening effect was compared using the agents shown in Table 2.
The results are shown in Table 2.

[0021]

[Table 2]

The Knei Pepeto extract in Table 2 was obtained by heating and reducing fruits in methanol, filtering and concentrating and drying.

As is clear from Table 2, the effect after exposure to sunlight is that the addition of the extract of Knei Pepeto prevents excessive deposition of melanin pigment and prevents darkening. Was recognized.

Example 1 Cream (Formulation) Stearic acid 5.0% by weight Stearyl alcohol 4.0 Isopropyl myristate 18.0 Glycerin monostearate 3.0 Propylene glycol 10.0 Knei-pepto methanol extract 0.01 Caustic potassium 0.2 Sodium bisulfite 0.01 Preservative Suitable amount Perfume Suitable amount Ion-exchanged water Residual (manufacturing method) Dissolve propylene glycol, Kney-pepetomethanol extract and caustic potassium in ion-exchanged water, heat and keep at 70 ° C (Aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the water phase, and after the addition is completed, the temperature is maintained for a while to cause a reaction. After that, homogenize with a homomixer,
Cool to 30 ° C with good stirring.

Example 2 Cream (Formulation) Stearic acid 2.0% by weight Stearyl alcohol 7.0 Hydrogenated lanolin 2.0 Squalane 5.0 2-Octyldodecyl alcohol 6.0 Polyoxyethylene (25 mol) cetyl alcohol ether 3.0 Glycerin monostearate ester 2.0 Propylene glycol 5.0 Knei-Pehet ethanol extract 0.05 Sodium hydrogen sulfite 0.03 Ethylparaben 0.3 Fragrance suitable amount Ion-exchanged water Residual (production method) Propylene in ion-exchanged water Add glycol,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the aqueous phase to carry out preliminary emulsification, and the mixture is uniformly emulsified with a homomixer and then cooled to 30 ° C. with thorough stirring.

Example 3 Cream (Formulation) Solid paraffin 5.0% by weight Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) sorbitan monolaurate 2 .0 Soap powder 0.1 Borax 0.2 Knei hepaetacetone extract 0.1 Sodium hydrogen sulfite 0.03 Ethylparaben 0.3 Fragrance suitable amount Ion-exchanged water Residual (production method) Soap powder and borax are added to ion-exchanged water , Melt by heating and keep at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the aqueous phase while stirring to carry out the reaction. After the reaction is completed, the mixture is uniformly emulsified with a homomixer, and after emulsification, the mixture is cooled to 30 ° C. with thorough stirring.

Example 4 Emulsion (formulation) Stearic acid 2.5 wt% Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3. 0 Triethanolamine 1.0 Carboxyvinyl polymer 0.05 (Brand name: Carbopol 941, BFGoodrich Chemical company) Knei Hepato acetate ethyl ester extract 0.01 Sodium bisulfite 0.01 Ethylparaben 0.3 Perfume proper amount Ion Exchanged water Residue (production method) A carboxyvinyl polymer is dissolved in a small amount of ion exchanged water (phase A). Polyethylene glycol 1500 and triethanolamine are added to the remaining ion-exchanged water, dissolved by heating, and kept at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase to perform preliminary emulsification, the phase A is added, and the mixture is uniformly emulsified with a homomixer. After the emulsification, the mixture is cooled to 30 ° C. while stirring well.

Example 5 Emulsion (Formulation) Microcrystalline wax 1.0% by weight Beeswax 2.0 Lanolin 20.0 Liquid paraffin 10.0 Squalane 5.0 Sorbitan sesquioleate 4.0 Polyoxyethylene (20 mol) ) Sorbitan monooleate 1.0 Propylene glycol 7.0 Knei hepatone Acetone extract 10.0 Sodium bisulfite 0.01 Ethylparaben 0.3 Fragrance Suitable amount Ion-exchanged water Residual (production method) Propylene glycol is added to ion-exchanged water In addition,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase is gradually added thereto, and the mixture is uniformly emulsified with a homomixer. After emulsification, cool to 30 ° C with good stirring.

Example 6 Jelly (formulation) 95% ethyl alcohol 10.0 wt% dipropylene glycol 15.0 polyoxyethylene (50 mol) oleyl alcohol ether 2.0 carboxyvinyl polymer 1.0 (trade name: Carbopol 940, BFGoodrich Chemical company) Caustic soda 0.15 L-Arginine 0.1 Knei-Phate 50% ethanol aqueous solution extract 7.0 2-Hydroxy-4-methoxybenzophenone sodium sulfonate 0.05 Ethylenediaminetetraacetate-3 sodium-2 Water 0.05 Methylparaben 0.2 Perfume Appropriate amount Ion-exchanged water Residual (production method) Carbopol 940 is uniformly dissolved in ion-exchanged water, while Knei Pepet 50% in 95% ethanol
An aqueous ethanol solution extract and polyoxyethylene (50 mol) oleyl alcohol ether are dissolved and added to the aqueous phase. Next, after adding other ingredients, caustic soda,
Thicken by neutralizing with L-arginine.

Example 7 Beauty Serum (Formulation) (Phase A) Ethyl Alcohol (95%) 10.0% by Weight Polyoxyethylene (20 mol) Octyldodecanol 1.0 Pantotenyl Ethyl Ether 0.1 Knei Hepato Methanol Extract 1.5 Methylparaben 0.15 (Phase B) Potassium hydroxide 0.1 (Phase C) Glycerin 5.0 Dipropylene glycol 10.0 Sodium bisulfite 0.03 Carboxyvinyl polymer 0.2 (trade name: Carbo Pole 940, BFGoodrich Chemical company) Purified water Residue (production method) Phases A and C are uniformly dissolved, and A is added to phase C
Add phases and solubilize. Next, after adding the phase B, filling is performed.

Example 8 Pack (formulation) (Phase A) 5.0% by weight dipropylene glycol Polyoxyethylene (60 moles) hydrogenated castor oil 5.0 (Phase B) Knei hepaetol methanol extract 0.01 Olive oil 5 0.0 Tocopherol acetate 0.2 Ethylparaben 0.2 Perfume 0.2 (Phase C) Sodium hydrogen sulfite 0.03 Polyvinyl alcohol 13.0 (Saponification degree 90, degree of polymerization 2,000) Ethanol 7.0 Purified water Residue (Manufacturing method) Phase A, phase B, and phase C are uniformly dissolved,
Add phase B to phase and solubilize. Next, this is added to the C phase and then filled.

Example 9 Solid Foundation (Formulation) Talc 43.1% by weight Kaolin 15.0 Sericite 10.0 Zinc white 7.0 Titanium dioxide 3.8 Yellow iron oxide 2.9 Black iron oxide 0.2 Squalane 8 0.0 isostearic acid 4.0 POE sorbitan monooleate 3.0 isocetyl octoate 2.0 Knei ethanol ethanol extract 1.0 preservative appropriate amount perfume appropriate amount (manufacturing process) Talc to black iron oxide powder component is sufficient with a blender After mixing, an oily component of squalane to isocetyl octoate, a Knei-pepetoethanol extract, an antiseptic and a fragrance are well kneaded, and then filled into a container and molded.

Example 10 Emulsion type foundation (cream type) (Formulation) (Powder part) Titanium dioxide 10.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Bengala 0.3 Black iron oxide 0.2 (Oil phase) Decamethylcyclopentasiloxane 11.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (Aqueous phase) Purified water 50.0 1,3-Butylene glycol 4.5 Kunei -Peperto ethanol extract 1.5 Sorbitan sesquioleate 3.0 Preservative proper amount Perfume proper amount (Production method) After heating and stirring the aqueous phase, the powder portion thoroughly mixed and pulverized is added and treated with a homomixer. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

[0034]

Industrial Applicability As described above, the whitening skin external preparation of the present invention has a melanin production inhibitory action and a tyrosinase activity inhibitory action, and is effective in treating pigmentation, stains, freckles, melasma, etc. after sunburn. It has excellent effects in lightening and whitening, and also in safety.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Hirohiko Sakamoto 1050 Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa Inside Shiseido Daiichi Research Center Co., Ltd.

Claims (3)

[Claims] 1. An external preparation for whitening skin, comprising an extract of a plant of the genus Kaempferia of the family Ging (Zingiberaceae). 2. A plant of the genus Kaempferia of the family Gingiber (Zingiberaceae) is Kune Pepet.
The external preparation for whitening skin according to claim 1, which is IPepet, scientific name: Kaempferia angustifolia). 3. The amount of the extract of a plant of the genus Kaempferia of the ginger (Zingiberaceae) genus is 0.00.
The external preparation for whitening skin according to claim 1 or 2, which is 5 to 20.0% by weight.