KR20200064240A - A composition comprising supercritical fluid extract of Sargassum horneri having antioxidant and skin whitening effect - Google Patents

Abstract

The present invention relates to an antioxidant or skin-brightening cosmetic composition comprising, as an effective component, a Sargassum horneri extract prepared by a supercritical fluid extraction method, and more specifically, compared to Sargassum horneri extracts obtained by existing extraction techniques typically using solvents, the Sargassum horneri extract prepared by a sequencing batch supercritical extraction method is more effective in terms of antioxidant activity, tyrosinase inhibitory activity, and melanin formation, and thus can prevent skin aging and has enhanced skin-brightening effects. In addition, the sequencing batch supercritical extraction method of the present invention is conducted preferably at 30-100°C under 100-600 atm using carbon dioxide, or conducted using mixed fluid containing a cosolvent or an auxiliary solvent. The cosolvent is used in the supercritical fluid extraction method, in addition to carbon dioxide, and is preferably any one selected from among mixed fluids obtained by mixing one or more selected from among ethanol, methanol, water, ethyl acetate, hexane, and dimethyl ether. In addition, the extract of the present invention includes, not only the extracts obtained through the aforesaid extraction method, but also extracts obtained by common purification processes. For example, the Sargassum horneri extract, which is the effective component of the composition of the present invention, may be extracted using a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. Also, mixed extracts prepared by extracting Sargassum horneri with the aforesaid solvents and combining the resulting extracts may be included in the range of the Sargassum horneri extract of the present invention.

Description

{A composition comprising supercritical fluid extract of Sargassum horneri having antioxidant and skin whitening effect} using supercritical fluid extraction method

The present invention relates to an antioxidant or skin whitening cosmetic composition containing an extract of Hoesengijaban prepared by supercritical fluid extraction as an active ingredient, and in particular, a Hoesengimojaban extract produced by a continuous batch supercritical extraction method is generally used. Compared with the extract of Hoeseng amoeba obtained by the conventional extraction method of applying a solvent, it is confirmed that it has anti-oxidation, inhibition of Tyrosinase activity and inhibition of Melanin production to prevent skin aging and enhance skin whitening, and is designed using this It relates to an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using the critical fluid extraction method.

The function of cosmetics is not only cleanliness and aesthetic function, but also free radicals due to external factors such as moisture, fine dust, pollutants, stress, ultraviolet rays, etc., and internal factors such as deterioration of immune cells and decrease in cell activity. Radical), which blocks or suppresses the occurrence of skin aging such as destruction of proteins, nucleic acids, and cell membrane lipids by free radicals and peroxides.

In addition, cosmetics that have whitening, skin wrinkle improvement and suppression, and UV protection to meet consumers' desire for high-functional cosmetics are continuously being developed. Among them, products for skin whitening and anti-aging have attracted much attention from consumers who want to keep their skin young.

Among them, as the standards for beauty have changed, interest in skin whitening has increased from teens to over 50s and over 60s, regardless of gender. Skin whitening is a functional expression that brightens and brightens the skin. Scientifically, it suppresses the production of melanin in the skin layer, thereby reducing the blackening of the skin. Melanin has a function of removing harmful radicals, thus protecting skin damage of modern people who have been exposed to harmful effects for a long time in their daily lives.

On the other hand, the epidermis of the skin that is excessively exposed to ultraviolet rays turns black to show the toxicity of melanin precursors as well as dark brown pigmentation, causing cell necrosis or death, skin aging, skin cancer, blemishes, freckles, and pigmentation.

Melanin is a dark brown pigment of a polymer present on the earth, which protects the skin from ultraviolet rays by absorbing more than a certain amount of ultraviolet rays. The melanin is produced not only by sunlight and ultraviolet rays, but also by prostaglandin or estrogen, and also affects the activation of the tyrosinase enzyme. Tyrosinase activity may also cause blemishes or freckles.

It forms 3,4-dihydroxyphenylalanine (L-DOPA) by oxidizing L-tyrosine in melanocytes by tyrosinase present in the cells. , Which in turn produces a substance called dopaquinone. The produced dopaquinone synthesizes melanin through an oxidation reaction itself.

Therefore, research and development must be conducted to suppress melanin production in order to prevent pigmentation and skin aging in the skin as the ultraviolet light increases and the exposure time increases, especially showing antioxidant and skin whitening used in food, medicine, and cosmetics from natural products. Since the movement to find raw materials is increasing in modern society, it is urgently desired to develop a material that is safe for living organisms and superior to existing antioxidant and whitening active materials.

Korean Registered Patent No. 10-1772494 (announced on August 30, 2017). "Whitening composition containing hoesaeng amethyst extract and its manufacturing method"

The present invention relates to a cosmetic composition excellent in effective skin aging prevention and skin whitening effect by using an intermittent continuous batch supercritical extraction system using a supercritical fluid as an extraction solvent when present at a critical pressure and temperature or higher, that is, a supercritical point or higher. In detail, it provides an antioxidant or skin whitening cosmetic composition containing an extract of Hoeseng amoebaban, which has excellent antioxidant and skin whitening effects, and has no side effects on skin by obtaining a Hoeseng amajaban extract produced by a supercritical extraction method. There is a purpose.

In addition, the present invention provides a cosmetic composition containing the extract of hoesam imovan obtained through supercritical extraction, which prevents skin aging by inhibiting antioxidant activity, inhibiting tyrosinase activity, and producing melanin, and has excellent skin whitening promoting effect. An object of the present invention is to provide an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using the critical fluid extraction method.

In order to solve the problems as described above, the present invention is an antioxidant containing the extract of hoejaengjaja using the supercritical fluid extraction method, characterized in that it contains an extract of hoejaengjajaban prepared by the intermittent continuous batch supercritical extraction method as an active ingredient or Provides a skin whitening cosmetic composition.

In addition, the continuous batch supercritical extraction method provides an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using a supercritical fluid extraction method characterized in that it is carried out using carbon dioxide at 30 to 100°C and 100 to 600 atmospheres. do.

In addition, the continuous batch supercritical extraction method is characterized in that, in addition to carbon dioxide, any one of a mixed fluid mixed with any one or more selected from ethanol, methanol, water, ethyl acetate, hexane and diethyl ether is used. It provides an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using supercritical fluid extraction method.

In addition, the content of the extract of hoesaengjapanja provides an antioxidant or skin whitening cosmetic composition containing hoesaengjajaban extract using a supercritical fluid extraction method, characterized in that it is 0.0001 to 1% by weight relative to the total weight of the composition.

In addition, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, soft lotion, nutrition lotion, nutrition cream , Massage cream, essence, pack, eye cream, cleansing cream, cleansing foam, cleansing water, moisture cream, fermentation cream, ampoule, shampoo, conditioner, spray or powder It provides an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using supercritical fluid extraction method.

As described above, the present invention relates to an antioxidant or skin whitening cosmetic composition containing as an active ingredient, a hoesaengjaban extract produced by a continuous batch supercritical extraction method, and in detail, a hoesaengjajaban extract prepared by a supercritical extraction method. It has the effect of providing an antioxidant or skin whitening cosmetic composition containing the extract of Hoeseng aza using the supercritical fluid extraction method, which has excellent antioxidant and skin whitening effects and has no side effects on the skin.

In addition, the present invention provides a cosmetic composition containing a supercritical extract of Hoeseng amethyceps, thereby inhibiting antioxidant activity, inhibiting Tyrosinase activity and inhibiting melanin production, and extracting Hoeseng Imoban extract using a supercritical fluid extraction method with excellent antioxidant and skin whitening effects. It has the effect of providing an antioxidant or skin whitening cosmetic composition containing.

1 is a graph showing the free radical scavenging activity of DPPH according to the extraction method of hoesaengjabanban.
Figure 2 is a graph showing the tyrosinase inhibitory activity according to the extraction method of hoesaengjabanban.
Figure 3 is a graph showing the inhibitory activity of melanin production according to the extraction method of hoesaengjabanban.

The present invention was studied based on an extraction method for establishing an extract excellent in antioxidant or skin whitening effects. As a result, the present invention was completed by confirming that the extract of Hoesengimojaban prepared through the supercritical fluid extraction method exhibits antioxidant and skin whitening effects by inhibiting antioxidant activity, inhibiting tyrosinase activity and producing melanin.

Sargassum horneri is a yellowish-brown plant belonging to the brown algae of the brown algae, the algae family, and is a year-round seaweed that forms a colony in the subtidal zone. It is mainly distributed in the east and south coasts of Korea, and is a species distributed universally in all coasts of Japan and China by currents toward the Japanese waters. In particular, it contains a large amount of fatty acids, omega-6, fucoidan, fucosterol, and fucoxanthin, as well as various physiologically active substances, so it has antioxidant, anti-cancer, anti-inflammatory, hypolipidemic, sunscreen, anti-diabetes, bone metabolism, and anti-pollution activity. It has been reported to have various efficacy activities.

In particular, similar to the characteristics of various marine organisms, hoejaengjapanja has a variety of biomechanisms that can respond to tissue and cell damage in a dry environment with ultraviolet rays, so it has high added value, such as blocking UV rays and producing moisturizing ingredients from polysaccharides. It is showing the possibility of utilization as a functional material, and it is thought that projects utilizing new materials of the future from marine resources, not land creatures, will continue to increase.

(shin YS. 2018.The research for cosmetic component about Algae complex including of Sargassum horneri and Enteromorpha prolifera extracts.The Korean Society of Cosmetics and Cosmetology. 8(2): 135-147), (Hwang EK, Lee SJ, Ha DS and Park CS. 2016. Sargassum Golden Tides in the Shinan-gun and Jeju Island, Korea.Korean Journal of Fisheries and Aquatics Sciences. 49(5): 689-693), (Li Y, Zhou C, Yan X, Zhang J , Xu J. 2016.Simultaneous analysis of ten phytohormones in Sargassum horneri by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry.Journal of Separation Science. 1-10)

Supercritical fluids have the properties of liquids and gases that the gas has when it reaches a critical point under high temperature and high pressure conditions, and chemically have a polarity similar to a non-polar solvent (J. Chromatogr., 479, 200-205, 1998). Due to these properties, supercritical fluids are used for extraction of fat-soluble materials. Examples using supercritical fluids include extraction of petroleum hydrocarbon compounds and polychlorine compounds from soil (J. Chromatogr.A., 845, 357-363, 1999), extraction of aromatic hydrocarbon compounds from edible fats (J. Chromatogr.A ., 882, 245-253, 2000), and extraction of fat-soluble vitamins from milk lipid components (J.Chromatogr.A., 874, 275-283, 2000).

The present invention is characterized in that it is an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban obtained by extracting hoesaengjabanja by continuous batch supercritical extraction method.

In the present invention, a continuous batch supercritical extraction method was used in order to maximize the antioxidant and skin whitening effects and effectiveness, and in order to enhance the antioxidant and skin whitening effects by increasing the skin absorption efficiency of the active ingredients, in the present invention, the supercritical extract of Hoeseng's cap It was used as an antioxidant and skin whitening cosmetic composition to maximize the effect through antioxidant activity, inhibition of tyrosinase activity, and inhibition of melanin synthesis.

The preferred dry weight ratio of the hoesan yibanban is preferably 5 to 30% by weight of the hoesaengjaban, by applying the continuous batch supercritical extraction method in addition to carbon dioxide in ethanol, methanol, water, ethyl acetate, hexane and diethyl ether It is preferable to use any one of the mixed fluids in which one or more selected ones are mixed.

The extracted sample of the present invention contains carbon dioxide, but through the intermittent continuous batch supercritical extraction system, the supercritical carbon dioxide is converted into gas and then converted into liquefied carbon dioxide by passing through a condenser, which can be reused as a separation tank. It is easy to separate from the extract.

The raw material of the extract, hoesaengjabanban, can be dried or used as it is. Preferably, it is appropriate to use it by natural drying or hot air drying, and most preferably by using hot air drying.

Referring to the following specific manufacturing example of the invention, a method for preparing the extract of the hoejaengjapan using the continuous batch supercritical extraction method is as follows: the haejajajajajaja raw material is dried after the hot air drying to less than 10% moisture content. Crushed to 2 mm.

The pulverized raw material is put into a supercritical extraction container and a supercritical fluid is maintained by applying a constant temperature to the carbon dioxide by using a high pressure pump to receive the extract through extraction of the target material. Alternatively, co-solvent, for example, ethanol is used as a co-solvent for carbon dioxide to supply and extract together.

According to one preferred embodiment of the present invention, the continuous batch supercritical extraction method of the present invention is preferably performed using carbon dioxide at 30 to 100°C and 100 to 600 atm, more preferably 40 to 70°C and 200 From 500 to 500 atmospheres, most preferably at 50° C. and 400 atmospheres using carbon dioxide.

According to a preferred embodiment of the present invention, the continuous batch supercritical extraction method of the present invention is performed using a mixed fluid in which a co-solvent or a co-solvent is additionally mixed with carbon dioxide.

The co-solvent is preferably used by selecting any one of mixed fluids in which one or more selected from ethanol, methanol, water, ethyl acetate, hexane and diethyl ether is added to carbon dioxide by applying a supercritical fluid extraction method. .

It is preferably 80% to 100% ethanol, and most preferably 95% ethanol (or alcohol).

The mixed fluid contains 3 to 40% by volume of the co-solvent based on the total volume, and most preferably 10% by volume.

In addition, the extract of the present invention includes not only the extract by the above-described extraction method, but also an extract that has been subjected to a conventional purification process. For example, the active ingredient of the composition of the present invention, hoesaengjaban, is a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. Extracted using or extracting hoesaengjaban using the above solvent, the mixed extract prepared by mixing it can also be covered in a range of hoesaengjaban extract of the present invention.

The roe-pee extract of the present invention may be prepared in a powder state by performing a further process such as distillation under reduced pressure, freeze drying or spray drying, but showing the properties of concentrated concentrate using a reduced pressure concentrator.

The cosmetic composition of the present invention enhances antioxidant activity and inhibits tyrosinase and melanan production to enhance the skin whitening effect, and such facts are specified in the following examples.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract obtained by supercritical fluid extraction of hoesan imijaban as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments, pigments And conventional auxiliaries such as perfumes, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, It can be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, the formulation of flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, pack, eye cream, cleansing cream, cleansing foam, cleansing water, moisture cream, fermentation cream, ampoule, shampoo, rinse, spray or powder Can be manufactured.

When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components It can be used by selecting.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Len glycol, 1,3-butyl glycol oil, polyoxyethylene cured castor oil, glycerol, glycerin, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleide, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/page-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, steareth-4, disetyl phosphate , Macadamia oil, carboxyvinyl polymer, xanthan gum or any one or more of fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, liquid diluents such as water, ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Same suspension, microcrystalline cellulose, hydroxyethylcellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , Sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, parpin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or tra Any one or more of Kant can be used.

When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide as a carrier component Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be selected and used.

Hereinafter, it will be described in detail by the production examples, examples, comparative examples, test examples and formulation examples of the present invention.

The following Preparation Examples, Examples, Comparative Examples, Test Examples, and Formulation Examples are merely illustrative of the present invention, and the present invention is not limited to the following Preparation Examples, Examples, Comparative Examples, Test Examples, and Formulation Examples.

[Preparation Example] Preparation of Hoeseng Imojaban Extract Using Extraction Method Using Organic Solvent Technique

The hoesaengjabanban was washed with purified water, dried, and then shredded within 2 cm to obtain a shredded sample. 50% ethyl alcohol was added so that the shredded hoejaengjapan was 1 kg/L, refluxed for 4 hours under reflux conditions, and cooled. After that, it was filtered with 6 μm filter paper (Whatman #3), and then rotary decompressed and freeze-dried to evaluate the efficacy.

[ Example ] Supercritical Using fluid extraction Aunt's Hat Preparation of extract

The hoesaengjabanban was washed with purified water, dried to maintain the moisture content of 10% or less, and then pulverized to a particle size of 2 mm to obtain a sample.

1 kg of the raw material of the crushed hoejaengjapan was added, and the carbon dioxide was prepared to be at a temperature of 50° C. and a pressure of 400 atm, and the isostatic pressure was maintained for 30 minutes. After 30 minutes, 95% ethanol was mixed with 10% by volume as a supercritical co-solvent and flowed with carbon dioxide for 120 minutes. The flow rate was set to 3 L/min and 0.3 L/min, respectively, for the carbon dioxide and the co-solvent, and after the co-solvent injection was completed, only the carbon dioxide was supplied for 120 minutes to proceed with the extraction of the subcritical system for a total of 270 minutes and to obtain an extract. The obtained extract was obtained using a rotary decompression concentrator to obtain a total of 25.0 g of supercritical hoesaengjaban extract.

[ Comparative example ] Hot water extraction method Used Hoeseng Preparation of single-hat extract

The hoesaengjabanban was washed with purified water, dried, and then shredded within 2 cm to obtain a shredded sample. Purified water was added so that the shredded hoejaengjaban was 1 kg/L, reflux was extracted for 4 hours at 60°C under reflux and cold-cooled. After that, it was filtered with 6 μm filter paper (Whatman #3), and then rotary decompressed and freeze-dried to evaluate the efficacy.

[ Test example One] DPPH (2,2- Diphenyl -One- picrylhydrazyl ) Radical Erasing ability

The scavenging ability for DPPH radicals was well mixed by adding 40 µl of each solvent fraction and 160 µl of 1,1-diphenyl-2-picryhydrazyl (DPPH) solution, reacted for 30 minutes in a cow, and measured the absorbance at 518 nm. The DPPH radical scavenging ability was confirmed. The free radical scavenging activity was expressed as a percentage compared to the sample-free group.

Aunt's Hat By extract DPPH Radical Erasing ability Confirm
division Concentration of hoesaengjabanban extract(㎍/㎖) 0 10 50 100 DPPH radical scavenging(%) Example 0 12.48±0.86 23.86±1.25 47.12±0.87 Manufacturing example 0 8.68±0.41 16.02±0.63 32.67±0.56 Comparative example 0 3.49±0.46 11.75±0.45 21.83±0.58 Ascorbic acid - - - 68.17±0.67

As shown in Table 1, it was confirmed that the embodiment of the present invention has superior DPPH radical scavenging ability than the hoesin azaban extract prepared in Preparation Example and Comparative Example.

[ Test example 2] Tyrosinase Active inhibition

Tyrosinase (Tyrosinase) activity inhibition experiment is to determine the effect of inhibiting the melanin activity of the corresponding material was measured by applying a method such as Yagi (Yagi) to measure the DOPA chrome by colorimetric method.

In this experiment, a reaction solution was prepared by adding 40 μl of a 1.5 mM L-Tyrosine solution to 120 μl of 0.1M phosphate buffer pH6.5 in a 96-well plate, and sample solution 10 to the reaction solution. After adding µl and reacting for 5 minutes, 10 µl of Mushroom tyrosinase (100 unit/ml) was added and enzyme reaction was performed at 37°C for 10 minutes. After 10 minutes, the dopachrome generated in the reaction solution was measured at 490 nm using a microplate counter, and Arbutin was used as a positive control in the same concentration as a comparison target for the test results.

[Calculation formula 2]

Aunt's Hat By extract Tyrosinase Active inhibition confirmation
division Concentration of hoesaengjabanban extract (㎍/㎖) 0 10 50 100 Tyrosinase inhibition rate (%) Example 0 18.24±0.81 27.45±0.35 42.57±0.61 Manufacturing example 0 16.50±0.52 22.93±0.41 29.83±0.95 Comparative example 0 7.45±0.63 12.36±0.62 18.71±1.12 Arbutin - - - 48.75±0.67

As shown in Table 2, the Examples of the present invention was able to confirm the tendency of the activity inhibitory rate of tyrosinase to decrease in a concentration-dependent manner than the extract of Hoeseng amoeba prepared in Preparation Examples and Comparative Examples (compared to the same concentration).

[Test Example 3] Melanin contents synthesis inhibition

B16F10 mouse melanoma cells were cultured in a 6-well plate at a concentration of 3.5Х10 ⁴ cells/well for 24 hours, and then, each well was prepared according to the concentration of α-MSH (melanocyte stimulating hormone) and prepared, prepared, compared Example samples were processed. After incubation for 48 hours, the medium was removed by washing twice with phosphate buffer saline (PBS), and the attached cells were transferred to an EP tube and centrifuged to obtain culture cell pellets. The shape of the precipitated B16F10 melanoma cells was photographed, and 1N NaOH solution added with DMSO was dissolved in melanin at 60°C for 1 hour, and then 200 μl was dispensed in 96-well plates to measure absorbance at 405 nm.

Confirmation of the inhibition rate of melanin production by hoesaengjabanban extract
division Concentration of hoesaengjabanban extract(㎍/㎖) 0 10 50 100 Melanin inhibition rate (%) Example 100 95.68±2.61 88.63±2.53 79.50±4.24 Manufacturing example 100 98.45±2.17 94.49±1.12 90.40±1.07 Comparative example 100 99.15±4.58 98.02±4.82 94.63±3.29 Arbutin 100 - - 82.54±3.28

As shown in Table 3, in Examples and Preparation Examples, it was confirmed that melanin synthesis was inhibited in a concentration-dependent manner. In particular, as shown in Example, the best melanin synthesis inhibitory effect was obtained in the extract of Hoeseng amoeba japonica using the first-time extraction method. there was.

On the other hand, it was confirmed that the melanin synthesis inhibitory effect was very low as compared with the control group, arbutin, which was extracted with hot water, which is a comparative example, did not have a large inhibition rate of melanin production compared to the same concentration.

[ Formulation example 1] of the present invention Supercritical Extraction Aunt's Hat Preparation of lotion containing extracts

Examples of formulations of cosmetic lotion (skin lotion) in the cosmetic composition containing the extract of hoesaengjaban in Example are shown in Table 4.

Aunt's Hat Composition of lotion containing extract number Raw material Content (% by weight) One Hoeseng aunt's keyboard extract (Example) 0.01 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyol ethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Coloring a very small amount 10 Spices a very small amount 11 Purified water Balance

To prepare the lotion, 2, 3, 4, and 8 were sequentially added to No. 11 listed in Table 4, stirred and dissolved, and then heated and dissolved at No. 5 at about 60° C., followed by aging.

[ Formulation example 2] of the present invention Supercritical Extraction Aunt's Hat Preparation of nutrient lotion containing extract

Examples of the formulation of the nutritional lotion in the cosmetic composition containing the extract of hoesaengjajaban in Example are shown in Table 5.

Aunt's Hat Containing extracts Nutritional lotion Configuration number Raw material Content (% by weight) One Hoeseng aunt's egg extract (Example) 0.01 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic monostearate glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl stearate/page-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Coloring a very small amount 15 Spices a very small amount 16 Purified water Balance

In order to prepare the nutrient lotion, 10, 11, 13 and 16 listed in Table 5 were mixed and stirred, heated to between 80 and 85° C., added to the production unit, and then acted as emulsifiers to 2, 3, 4, 5, 6 , 7, 8, 9 and 12 were heat-dissolved between 80 and 85°C, and then emulsified. After the emulsification, the mixture was cooled to 50°C with stirring using a stirrer, and then added 15 times. After cooling to 45°C, 14 times were added, and once at 35°C, the mixture was cooled to 25°C and aged.

[Formulation Example 3] Preparation of fermented cream containing hoesaengjaban using the supercritical extraction method of the present invention

Table 6 shows an example of the formulation of the cream in the cosmetic composition containing the extract of hoesaengjaban in Example.

Aunt's Hat Composition of fermented cream containing extract number Raw material Content (% by weight) One Hoeseng aunt's egg extract (Example) 0.01 2 glycerin 10.0 3 Propylene glycol 10.0 4 1,2-hexanediol 2.0 5 Cetyl alcohol 1.5 6 Carnauba wax 1.5 7 PPG-14 butyl ether 1.5 8 Microcrystalline wax 0.5 9 Polysorbate 0.8 10 PG-100 Stearate 0.75 11 Glyceryl stearate 0.75 12 Polysorbate 60 0.5 13 Ammonium acroyl dimethyl taurate / VP copolymer 0.3 14 Disodium LED 0.05 15 Purified water Balance

In order to prepare the fermented cream, 2, 3, 14 and 15 listed in Table 6 are completely dissolved while mixing and stirring in the main beaker. 5, 6, 7, 8, 9, 10, 11 and 12 are heated to 80° C. to dissolve the raw materials and slowly added to the main beaker. This was emulsified while stirring, and 13 and 15 were added to the main beaker, stirring evenly and cooling the temperature to 45°C. Thereafter, 1 and 4 were added to the main beaker, stirred for 5 minutes with a homomixer, and then cooled to 25°C to finish cream production.

[ Formulation example 4] of the present invention Supercritical Extraction Hoesaengjabanban Preparation of containing essence

Table 7 shows an example of the formulation of the essence in the cosmetic composition containing the extract of hoesaengjabanban of the embodiment.

Aunt's Hat Extract Containing containing Composition of essence number Raw material Content (% by weight) One Hoeseng aunt's egg extract (Example) 0.01 2 Cito sterol 1.7 3 Polyglycerin 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Shotgun 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

To prepare the essence, those prepared by homogenizing 2, 3, 4, 5 and 6 in Table 7 at a constant temperature are referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid was mixed with 1, 7, 8, and 14 and homogenized at a constant temperature to pass through the microfluidizer, followed by slowly inoculating the 9 at a constant temperature, homogenized, and then the microfluidizer again It was passed through again, and 10, 11, 12, and 13 were added to disperse to stabilize and aged.

The present invention is only an example for an antioxidant or skin whitening cosmetic composition containing an extract of Hoeseng imijaban prepared by a supercritical fluid extraction method as an active ingredient, and the present invention is not limited to the above example but various different forms It can be implemented as, only this embodiment is to provide the complete disclosure of the present invention, and to provide those who have ordinary knowledge in the art to which the present invention pertains to the scope of the invention. It is only defined by the scope of the claims.

Claims (5)

Antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using supercritical fluid extraction method, characterized in that it contains hoesaengjajaban extract produced by the intermittent continuous batch supercritical extraction method as an active ingredient. According to claim 1,
The continuous batch supercritical extraction method is an antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjajaban using a supercritical fluid extraction method, characterized in that carried out using carbon dioxide at 30 to 100 ℃ and 100 to 600 atmospheres. According to claim 1,
The continuous batch supercritical extraction method is characterized in that it is used by selecting any one of a mixed fluid mixed with any one or more selected from ethanol, methanol, water, ethyl acetate, hexane and diethyl ether in addition to carbon dioxide Antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using the critical fluid extraction method. According to claim 1,
Antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using supercritical fluid extraction method, characterized in that the content of hoesaengjaban extract is 0.0001 to 1% by weight relative to the total weight of the composition. According to claim 1,
The cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, flexible lotion, nutrition lotion, nutrition cream, massage Candles characterized by being formulated with any one or more selected from the group consisting of cream, essence, pack, eye cream, cleansing cream, cleansing foam, cleansing water, moisture cream, fermentation cream, ampoule, shampoo, rinse, spray or powder Antioxidant or skin whitening cosmetic composition containing the extract of hoesaengjaban using the critical fluid extraction method.

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