KR20130088912A - Skin external composition containing tangeretin and egcg - Google Patents

Abstract

PURPOSE: An external use skin composition is provided to have a safe skin whitening effect without skin irritation using tengeretin contained in a citrus peel extract and epigallocatechin gallate (EGCG) contained in a green tea extract. CONSTITUTION: An external use skin composition contains tangeretin and EGCG. Tangeretin is contained in a citrus peel extract. EGCG is contained in a green tea extract. The composition contains 0.001-10 wt% of a citrus peel extract and 0.001-99.9 wt% of the green tea extract. [Reference numerals] (AA) p-value of each value treated with tangeretin < 0.05

Description

Skin external composition containing tangeretin and EGCG}

The present invention relates to an external composition for skin having a whitening effect by containing tengerretin and EGCG.

Many factors are involved in determining the skin color of humans, including the activity of melanocytes that make melanin, the distribution of blood vessels, the thickness of the skin and the presence or absence of pigments in the body, such as carotenoids and bilirubin. It is important.

Especially, the most important factor is the black pigment called melanin which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

Today, Asian women prefer white and clean skin like white jade, and this is an important standard of beauty, increasing the demand for treatment and cosmetic problems for hyperpigmentation.

In response to this demand, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof, or substances having tyrosinase inhibitory activity have been used in combination with cosmetics or pharmaceuticals, but they are insufficient. Its use has been limited due to its whitening effect, safety issues on the skin, formulation and stability problems when formulated in cosmetics, and the like. Therefore, there is an urgent need for the development of a composition that is safer on the skin and has an excellent whitening effect.

The inventors of the present invention, through repeated and various experiments, it was confirmed that by using the tangerintin (tangeretin) contained in the citrus peel extract and EGCG contained in the green tea extract can provide a safe and excellent whitening effect on the skin This invention was completed.

Accordingly, it is an object of the present invention to provide an external composition for skin containing tengerretin and EGCG as an active ingredient.

In order to achieve the above object, the present invention provides a whitening skin external composition comprising both a tenger retin and EGCG as an active ingredient.

The composition of the present invention exhibits an excellent skin whitening effect by further containing EGCG in the tengeretine, through which it can be variously used in the cosmetic or pharmaceutical field.

Figure 1 is a diagram showing the test results of the antioxidant synergistic effect of tengerretin and EGCG.

The external preparation composition for skin of the present invention contains both tengeretine and EGCG as an active ingredient. In addition, the present invention provides a skin external preparation composition having no skin irritation and safe skin whitening effect by using EGCG contained in the tangerine and green tea extract contained in the citrus peel extract.

Hereinafter, the present invention will be described in detail.

Tengerretin (Tangeretin) used in the present invention is preferably contained in the citrus peel extract. Tengeretine is a citrus flavonoid-based O-polymethoxylated flavones (O-polymethoxylated flavones) structure, the tenseretin (tangeretin) used in the present invention is a citrus flavonoid-based O-polymethoxylated flavones (O-polymethoxylated flavones) ) Has the structure Among the flavones, polymethoxyflavones are rarely found in other vegetables and fruits and are a characteristic component of citrus fruits, in which five methoxy groups are substituted for flavonoids. Tengeretine is represented by the following formula (1).

In one embodiment of the present invention, the citrus peel extract may be contained in an amount of 0.001 to 40% by weight based on the total weight of the composition. If the citrus peel extract is contained in less than 0.001% by weight, the efficacy and effect by the above ingredients is weak, and if it exceeds 40% by weight, there is a problem of safety or formulation stability due to skin irritation.

EGCG (Epigallocatechin gallate) used in the present invention is preferably contained in green tea extract. EGCG has a strong whitening effect as a constituent of tannin or polyphenols, and is represented by the structure of Formula 2 below.

In addition, the green tea extract in one embodiment of the present invention may be included in 0.001 to 40% by weight based on the total weight of the composition. If the green tea extract is included in less than 0.001% by weight, the efficacy and effect by the above ingredients are insignificant, and when the green tea extract exceeds 40% by weight, there are safety problems such as skin irritation or stability problems in formulation.

The method for preparing the tangerine extract containing tangeretine and the green tea extract containing EGCG used in the present invention is not particularly limited, and in one embodiment of the present invention, the extracts are extracted by immersing the raw material in an organic solvent and then filtering the extract. The concentrate obtained by depressurizing and concentrating can be used. Or stagnation, premises, periodic and flow extracts prepared by drying the concentrate again can be used. The deposit can be deposited by any method such as cold sedimentation, percolation, and warm needle using citrus peel or green tea solvent to obtain a deposit containing the active ingredient. The organic solvent may be one or more selected from C ₁ to C ₄ anhydrous or hydrous alcohols, acetone, ethyl acetate, chloroform, glycerin, propylene glycol and butylene glycol, but is not limited thereto.

On the other hand, in one embodiment of the present invention, the composition may be for preventing skin erythema or improving skin tone. In addition, in one embodiment of the present invention, the composition may prevent skin aging, increase skin elasticity, improve skin wrinkles, improve color, shrink pores, reduce skin irritation, improve homeostasis, slimming, atopy, antibacterial antibacterial, allergy, elasticity, acne, Regeneration, sebum, waste products, keratin improving effect, skin convergence and trimming effect, as well as obesity, diabetes can be improved, but is not limited thereto.

The external preparation composition for skin according to the present invention is generally a concept covering the whole composition used for external application of the skin, and may be, for example, a cosmetic composition or a pharmaceutical composition.

The cosmetic composition may include, for example, a basic cosmetic, a makeup cosmetic, a hair cosmetic, a body cosmetic, and the like, and its formulation is not particularly limited and may be appropriately selected as desired.

For example, the cosmetic composition is formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. It may be, but is not limited thereto. More specifically, softening cream, nourishing cream, lotion, body lotion, nourishing cream, massage cream, moisturizing cream, hand cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, gel, patch, oil in water Foundation cosmetics such as (W / O) and water-in-oil (O / W), color cosmetics such as lipstick, makeup base or foundation, cleaning agents such as shampoo, rinse, body cleanser, toothpaste or mouthwash, hair tonic, It may be formulated into a cosmetic composition for hair, such as a hair dressing agent such as a gel or a mousse, a wool or a hair dye.

The cosmetic composition contains a cosmetically acceptable medium or base. It may be in any form suitable for topical application, for example as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) and / In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

In one embodiment of the present invention, the cosmetic composition may further contain a thickener. The thickener included in the cosmetic composition of the present invention is methyl cellulose, carboxy methyl cellulose, carboxy methyl hydroxy guanine, hydroxy methyl cellulose, hydroxyethyl cellulose, carboxy vinyl polymer, polyquaternium, cetearyl alcohol, stearic acid, Carrageenan and the like can be used, and preferably, at least one of carboxy methyl cellulose, carboxy vinyl polymer and polyquaternium can be used, and most preferably, carboxy vinyl polymer.

In one embodiment of the present invention, the cosmetic composition may contain a variety of suitable bases and additives as necessary, the type and amount of these components can be easily selected by the inventor. If necessary, it may contain an acceptable additive, for example, may further include components such as preservatives, pigments, additives and the like conventional in the art. Preservatives may be specifically phenoxyethanol (Phenoxyethanol) or 1,2-hexanediol (1,2-Hexanediol) and the like, the pigment may be an artificial flavor or the like.

And, in one embodiment of the present invention, the cosmetic composition may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, any of the above components can be mix | blended within the range which does not impair the objective and effect of this invention.

In one embodiment of the present invention is a parenteral in the form of a solid, semi-solid or liquid by adding a commercially available inorganic or organic carrier with the tangerine extract containing citrus peel and green tea extract containing EGCG as an active ingredient It may be formulated as a dosage agent. For parenteral administration, the preparation may be a transdermal dosage form selected from the group consisting of drops, ointments, lotions, gels, creams, patches, sprays, suspensions and emulsions, but is not limited thereto.

In the external composition for skin according to each formulation, other ingredients other than the composition of the present invention described above may be appropriately selected and blended by those skilled in the art according to the formulation or use purpose of the external skin preparation, and in this case, simultaneously applied with other raw materials. If you do, synergistic effects may occur.

In addition, when the composition according to the present invention is used as a pharmaceutical composition, it may further contain a pharmaceutical adjuvant and other therapeutically useful substances such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts or buffers for osmotic control. And may be formulated in a variety of oral or parenteral dosage forms according to conventional methods.

It is to be understood that the actual dosage of the active ingredient should be determined in light of several relevant factors such as the severity of the symptom, the route of administration chosen, the age, sex, weight and health of the subject.

In general, the dosage of the active ingredient ranges from 0.001 mg / kg / day to approximately 2000 mg / kg / day. More preferred dosages are from 0.5 mg / kg / day to 2.5 mg / kg / day.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

Reference Example 1 Preparation of Citrus Peel Extract Containing Tensor Retin

The citrus peels are washed with purified water and then dried. The dried citrus peel is pulverized and the active ingredient is extracted by cold extraction at room temperature for 40 to 48 hours. The extracted content was first filtered through a 250μm mesh, and the supernatant was taken through centrifugation. The contents are again filtered sequentially with filter paper of 3 μm, 1 μm, and 0.5 μm mesh. Add 1,3-butylene glycol 10%, phenoxyethanol 0.5%, ethylhexylglycerin 0.05%, and filter again with 0.2 ~ 0.3μm filter paper to obtain citrus peel extract. .

Reference Example 2 Preparation of Green Tea Extract Containing EGCG

The green tea leaves are washed with purified water and then dried. Crush the green tea leaves, which have been drained, and extract the active ingredient through cold extraction at room temperature for 40 to 48 hours. The extracted content was first filtered through a 250μm mesh, and the supernatant was taken through centrifugation. The contents are again filtered sequentially with filter paper of 3 μm, 1 μm, and 0.5 μm mesh. Add 1,3-butylene glycol (10%), phenoxyethanol (0.5%), phenoxyethanol (0.5%), ethylhexylglycerin (0.05%) and filter again with 0.2 ~ 0.3 μm filter paper to obtain green tea extract.

[Test Example 1] melanin production inhibitory effect

MEM containing 10% fetal bovine serum in human melanoma cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan) (Minimum Essential Medium) was incubated under 37 ℃, 5% CO ₂ conditions. The cultured cells were spread over 75 flasks so that the number of cells was 3 × 10 ⁵ per flask, and waited for the cells to adhere to the wall for one night. After confirming that the cells adhered well, the medium was tested. Change to fresh medium containing ppm. At this time, the control group using a medium not added, citrus peel extract and green tea extract was used in Example 1, Koji acid known to exhibit a whitening effect Comparative Example 1, citrus peel extract Comparative Example 2, green tea extract It was set as the comparative example 3. Once every two to three days, the cells were incubated with a fresh medium containing the sample until the cells were filled in the flask. When the cells were grown, the cells were collected and the cell colors of Examples 1 and 3 were compared. In addition, the culture medium was removed, washed with PBS and dissolved with 1N sodium hydroxide to measure the absorbance at 500 nm and calculated the inhibition rate of melanin production according to Equation 1 below, the results are shown in Table 1 below.

Test substance Melanin production inhibition rate (%) Example 1 25.1 Comparative Example 1 28.3 Comparative Example 2 5.3 Comparative Example 3 8.7

From the results of Table 1, when using Example 1 in combination with citrus peel extract and green tea extract, it can be seen that it has a melanin production inhibition rate similar to kojic acid is known to have a very good whitening effect. In addition, it showed an excellent melanin production inhibition rate of 4 to 5 times than Comparative Examples 2 and 3 using citrus peel extract and green tea extract, respectively. This is due to the synergistic effect of EGCG contained in the tangerine and green tea extract in citrus peel extract melanin production is increased.

Production Example 1 and Comparative Production Examples 1 to 4

 According to the composition shown in Table 2 below, the cosmetic compositions of Preparation Example 1 and Comparative Preparation Examples 1 to 4 were prepared. Looking at the manufacturing process in detail, the raw materials 1 ~ 9 were mixed and dissolved at 70 ℃ to give a water part. On the other hand, raw materials 10-15 were melt | dissolved at 70 degreeC, and it was set as the oil part. An oil part was added to the above water part, stirred with a homomixer (Tokushu Kika Co., Ltd., Japan) and emulsified first, and the raw materials 16 and 17 were added to increase. After removing the bubbles, the mixture was cooled to room temperature to prepare a cosmetic composition of Preparation Example 1 and Comparative Preparation Examples 1 to 4 (unit: wt%).

ingredient Production Example 1 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 1. Purified water Balance Balance Balance Balance Balance 2. Glycerin 3.0 3.0 3.0 3.0 3.0 3. Butylene Glycol 5.0 5.0 5.0 5.0 5.0 4. Beta Glucan 5.0 5.0 5.0 5.0 5.0 5. Hyaluronic Acid Extract 3.0 3.0 3.0 3.0 3.0 6. Carbomer 0.1 0.1 0.1 0.1 0.1 7. Citrus Pea Extract One - - 2 - 8. Green Tea Extract One - - - 2 9. Kojisan - - 2 - - 10. Caprylic / Capric Triglycerides 8.0 8.0 8.0 8.0 8.0 11. Hydrogenated Polydecene 5.0 5.0 5.0 5.0 5.0 12. Cetearyl Glucoside 1.5 1.5 1.5 1.5 1.5 13. Sorbitan Stearate 0.4 0.4 0.4 0.4 0.4 14. Cetearyl Alcohol 1.0 1.0 1.0 1.0 1.0 15. Preservatives Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 16. Incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 17. Triethanol Amine 0.1 0.1 0.1 0.1 0.1

Test Example 2 Whitening Effect on Human Skin

To examine the whitening effect and skin irritation on human skin, an opaque tape with 6 holes of 1.5 cm diameter was attached to the upper forearm area of 30 healthy men and women aged 35.2 years old. Ultraviolet light (UVB) of 1.5 ~ 2 times the minimum Erythema Dose of the subject was irradiated to induce blackening of the skin, and after 2 months of application of test substances, the contrast of the skin was measured using a colorimeter. . To these subjects, the cosmetics of Preparation Example 1 and Comparative Preparation Examples 1 to 4 were applied twice daily in the morning and evening.

The determination of the effect was made by determining the value "L" For reference, Korean skin color that is not artificially burned generally shows a value of 50-70. The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L * a * b * colorimetric system is used for displaying the color, but in the present invention, the L * value (brightness) is mainly used as an index. The L * value was calibrated with a standard whiteboard, and the pigmentation part was measured evenly by repeating the measurement five times or more at one site. The difference in skin color (ΔL *) at the start and completion of the application was calculated according to Equation 2 below, which is shown in Table 3 below. The whitening effect is determined by comparing ΔL * between the sample application site and the control site. When the value of ΔL * is about 2, the whitening effect of the deposited pigment is clear. .

When the test substance is applied, the L value is gradually increased, and the comparison between the test substances is different from the color of the skin (starting application and completion of two months after application). L value is gradually increased when there is a comparison, and the comparison between the test materials was calculated according to the above equation (ΔL) of the skin color difference between the start time and the completion time (after two months of application). The results are shown in Table 3 below.

Test Example 3 Investigation of Skin Irritability on Human Skin

The skin irritation was compared after the cosmetics of Preparation Example 1 and Comparative Preparation Examples 1 to 4 were used for two months on the subjects of Test Example 2. At this time, the criterion is compared by giving the subjects a score of 1 to 5 according to the degree of stimulus, the closer to 1 means no stimulus, the closer to 5 means more stimulus, each average score is shown in the following table 3 is shown.

Test substance Whitening effect (△ L) Skin irritation Production Example 1 2.0 1.4 Comparative Preparation Example 1 0.02 1.14 Comparative Production Example 2 2.4 1.5 Comparative Production Example 3 0.4 1.8 Comparative Production Example 4 0.5 2.1

As can be seen from the results in Table 3, the whitening effect is superior to Comparative Preparation Example 1 in the cosmetic preparation of Preparation Example 1, which uses a mixture of the citrus peel extract and green tea extract according to the present invention, and at the same time, skin irritation uses the same amount of kojic acid. It was confirmed that it has a similar value as when. In addition, it was found that Preparation Example 1, which used a mixture of citrus peel extract and green tea extract, was markedly superior to the cosmetic preparations of Comparative Preparation Examples 3 and 4, which was also about 4 to 5 times or more, and also significantly reduced skin irritation. Could. This is believed to be due to the synergistic effect of the mixing of the tangeretine contained in the citrus peel extract and EGCG contained in the green tea extract.

Test Example 4 Change in Angular Mass

In order to examine the effect of reducing the angular mass of the whitening cosmetic composition according to the present invention, the change in the skin angular mass of the cosmetics prepared in Preparation Example 1 and Comparative Production Examples 1 to 4 was examined.

After applying the cosmetic composition of Preparation Example 1 and Comparative Preparation Examples 1 to 4 on the inner half of 50 males and females in their 20s and 30s without skin diseases, Charm view (Moritex, Japan) The amount of keratin decrease was measured using. The initial skin angular mass was measured and used as the basic value at the constant temperature and humidity conditions (24 ° C., humidity 40%) before starting the coating, and the change after 24 hours was measured, and the results are shown in Table 4 below. It was.

Skin angular mass change according to whitening cosmetic composition (%) Test substance Before application 24 hours later Rate of change (%) Production Example 1 20.9 16.1 0.18 Comparative Preparation Example 1 20.1 19.9 0.01 Comparative Production Example 2 20.3 17.6 0.13 Comparative Production Example 3 21.0 20.3 0.03 Comparative Production Example 4 20.5 19.6 0.04

From the results of Table 4, Preparation Example 1, a whitening cosmetic composition according to the present invention was found to have a superior skin exfoliation effect compared to Comparative Preparation Examples 1-4. In addition, in the case of Preparation Example 1 prepared by mixing the citrus peel extract and green tea extract, it was found that the most excellent skin exfoliation effect appeared by synergistic effect.

Therefore, it was confirmed that the composition further comprising EGCG in the tenderretin according to the present invention can provide excellent skin safety by minimizing skin irritation with an excellent whitening effect on the skin.

Test Example 5 Evaluation of Free Radical Removability: DPPH Assay

DPPH (2,2-Diphenyl-1-picrylhydrazyl, Sigma, D9132) was used to confirm the free radical scavenging ability by using a mixture of tenerretin and EGCG DPPH assay. When purple, DPPH reacts with antioxidants to receive hydrogen electrons from antioxidants, changing their color and decreasing their absorbance at 517 nm.

First, DPPH was prepared, and before starting the experiment, it was dissolved in ethanol to 100 μm, sonicated, and completely dissolved, and then treated materials were prepared in the following order.

1) Tengeretine 10% solution (solvent: dimethyl sulfoxide (DMSO)) is diluted in ethanol according to the treatment concentration.

2) The number of treatments of the same concentration is repeated three times for each substance treatment concentration section.

3) The concentration is reduced to 1/20 when reacting with DPPH solution. Prepare the material at 20 times the concentration to be checked.

Thereafter, the treated material was placed in a 96-well plate at 10 μl for each treatment concentration. As a positive control, 10 μl of vitamin C was added at 2.5, 5, and 10 ppm concentrations, respectively. 10 μl of ethanol was added to the control group. Then, 190 μl of 100 μm DPPH was added, and if the material had a color, it could affect absorbance. Thus, an experimental group of 190 μl of ethanol was added to 10 μl of material.

The material reaction was stored in an incubator at 37 ° C. for 30 minutes, and then the absorbance at 517 nm was measured using a spectrophotometer (Spectra Max 190). Absorbance value (OD) was measured according to the concentration change when a mixture of green tea extract containing vitamin C, EGCG, citrus peel extract containing tengeretine and citrus peel extract containing tengeretine was mixed with green tea extract containing EGCG. 1 is shown.

The greater the antioxidant power of the substance, the lower the absorbance value. Compared with the control group, the concentration of the substance that reduces the absorbance value in half (IC50: 50% Inhibitory activity) was calculated. The smaller the IC50 value, the greater the antioxidant power.

As shown in Figure 1 it was confirmed that there is an antioxidant effect with a significant difference when treating both together compared to when treated with EGCG and tengeretine alone at all concentrations. Therefore, the antioxidant synergistic effect of Tensor Retin and EGCG prevented the skin whitening and prevented the oxidation of the skin and gave the skin vitality.

Hereinafter, the formulation examples of the cosmetic composition and the pharmaceutical composition according to the present invention will be described, but can be applied in various formulations in addition to the following examples, which are intended to explain in detail only, not intended to limit the present invention.

Formulation Example 1 Flexible Lotion (Skin Lotion)

To a flexible lotion was prepared in a conventional manner according to the composition shown in Table 5.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green Tea Extract (EGCG) 1.0 glycerin 3.5 Oleyl alcohol 1.5 ethanol 5.5 Polysorbate 80 3.2 Carboxyl vinyl polymer 1.0 Butylene glycol 2.0 Propylene glycol 2.0 Preservative, fragrance Suitable amount Purified water Balance system 100

Formulation Example 2 Nutritional Lotion (Milk Lotion)

To the nutritional lotion was prepared in a conventional manner according to the composition shown in Table 6.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green Tea Extract (EGCG) 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / Capric Reglycerides 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, fragrance Suitable amount Purified water Balance system 100

Formulation Example 3 Nutrition Cream

To prepare a nutritious cream in a conventional manner according to the composition shown in Table 7.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green tea extract 1.0 glycerin 3.5 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Reglycerides 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 4] Massage cream

To prepare a massage cream in a conventional manner according to the composition shown in Table 8.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green Tea Extract (EGCG) 1.0 glycerin 8.0 Butylene glycol 3.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 5] Pack

To prepare a pack in a conventional manner according to the composition described in Table 9.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green tea extract 1.0 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative Suitable amount Preservative, fragrance Suitable amount Purified water Balance system 100

Formulation Example 6 Agent for Local Administration (Patching Agent)

The patch was prepared in a conventional manner according to the composition described in Table 10 below.

ingredient Content (% by weight) Citrus Peel Extract (Tensor Retin) 1.0 Green tea extract 1.0 Beta-1,3-glucan 3.0 Diethylamine 0.7 Sodium sulphate 0.1 Polyoxyethylene lauryl ether (E.O = 9) 1.0 Polyhydroxyethylenecetylstearyl ether (Cetomacrogol 1000) 1.0 Viscous paraffin oil 2.5 Caprylic Acid Ester / Capric Acid Ester (Cetiol LC) 2.5 Polyethylene glycol 400 3.0 Polyacrylic Acid (Carbopol 934P) 1.0 Purified water Balance system 100

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A skin external composition comprising tangertin and EGCG (Epigallocatechin gallate) together. The composition for external application for skin according to claim 1, wherein the tengeretine is contained in a citrus peel extract. The composition for external application for skin according to claim 1 or 2, wherein the EGCG is contained in green tea extract. The composition for external application for skin according to claim 2, wherein the citrus peel extract is contained in an amount of 0.001 to 10% by weight based on the total weight of the composition. The composition for external application for skin according to claim 3, wherein the green tea extract is contained in an amount of 0.001 to 99.9% by weight based on the total weight of the composition. The external preparation composition for skin according to claim 1 or 2, wherein the composition is for skin whitening.

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