JP2013155174A - Skin external preparation composition containing tangeretin and egcg - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin external preparation composition that is safe and has a skin whitening effect while causing no skin irritation.SOLUTION: A skin external preparation composition contains both of tangeretin and EGCG (epigallocatechin gallate). The tangeretin is preferably included in the extract of orange peel, and the EGCG is preferably included in the extract of green tea. By using tangeretin included in the extract of orange peel and EGCG included in the extract of green tea, the obtained skin external preparation composition has an excellent effect of skin whitening, and is useful for cosmetics and pharmaceutical products.

Description

  The present invention relates to a skin external preparation composition having a whitening effect by containing tangeretin and EGCG.

  Various factors are involved in determining human skin color. Among them, the activity of melanocytes that produce melanin pigment, distribution of blood vessels, skin thickness, and inclusion of pigments inside and outside the human body such as carotenoids and bilirubin The presence or absence of this is an important factor.

  Among them, the most important factor is a black pigment called melanin that is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of this melanin pigment is influenced by genetic factors, hormone secretion, physiological factors related to stress, and environmental factors such as ultraviolet irradiation.

  Melanin pigment produced from melanocytes in the body skin is a phenolic polymer substance in the form of a complex of black pigment and protein, blocking the ultraviolet rays irradiated from the sun, and the skin organs below the dermis In addition to protecting and capturing free radicals generated in the skin, it is responsible for protecting the proteins and genes in the skin.

  As described above, melanin generated by stimulation such as external stress in the skin is a stable substance that does not disappear until the stress disappears until it is discharged to the outside through skin keratinization. However, more melanin than necessary can cause hyperpigmentation such as stains, buckwheat and moles, resulting in poor cosmetic results.

  Today, Oriental women prefer white and clean white skin and make this an important standard of beauty, which has led to an increasing demand for treatment and cosmetic problems for hyperpigmentation. .

  In response to such demands, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof, or substances having tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals. Its use is limited by insufficient whitening effect, safety problems on the skin, dosage forms appearing when blended into cosmetics and stability problems. Accordingly, there is an urgent need for the development of a composition that has an excellent whitening effect while being safer on the skin.

  The inventor of the present invention repeats various experiments and uses both tangeretin contained in the mandarin peel extract and EGCG (Epigallocatechin gallate) contained in the green tea extract to the skin. It has been confirmed that an excellent whitening effect can be provided while being safe, and the present invention has been completed.

  Accordingly, an object of the present invention is to provide a skin external preparation composition containing tangeretin and EGCG as active ingredients.

  In order to achieve the above object, the present invention provides a skin whitening composition for whitening containing both tangeretin and EGCG as active ingredients.

  The composition of the present invention shows an excellent skin whitening effect by further containing EGCG in tangeretin, and can be used in various ways in the cosmetics or pharmaceutical fields.

It is the figure which showed the test result of the antioxidant synergy effect of tangeretin and EGCG.

  The skin external preparation composition of the present invention contains both tangeretin and EGCG as active ingredients. In addition, the present invention provides a skin external preparation composition that has a skin whitening effect safely without causing irritation to the skin by using tangeretin contained in a citrus peel extract and EGCG contained in a green tea extract. I will provide a.

Hereinafter, the present invention will be described in detail.
The tangeretin used in the present invention is preferably contained in a citrus peel extract. The tangeretin is a citrus flavonoid type and has a structure of O-polymethoxylate flavones, and the tangeretin used in the present invention is a citrus flavonoid type, and O-polymethoxylate flavone (O- (polymethylated flavones). Among flavones, polymethoxyflavones are rarely found in other vegetables and fruits, and are characteristic components of mandarin oranges. Five methoxys are substituted for flavonoids. It is a substance that has been. Tangeretin is represented by the following chemical formula 1.

  In one embodiment of the present invention, the citrus peel extract may be included in an amount of 0.001 to 40% by mass based on the total mass of the composition. When the citrus peel extract is contained in an amount of less than 0.001% by mass, the effects and effects of the above components are weak. This is because there is a problem.

  The EGCG used in the present invention is preferably contained in a green tea extract. EGCG is a constituent of tannin or polyphenol, has a strong whitening effect, and is represented by the structure of the following chemical formula 2.

  Moreover, in one Example of this invention, the said green tea extract may be contained in 0.001-40 mass% with respect to the composition total mass. If the green tea extract is contained in less than 0.001% by mass, the effects and effects of the above ingredients are weak, and if it exceeds 40% by mass, there are safety problems such as skin irritation or stability problems in the dosage form. Because there is.

The method for producing a mandarin peel extract containing tangeretin and a green tea extract containing EGCG used in the present invention is not particularly limited, and in one embodiment of the present invention, the extract comprises: After the raw material is immersed in an organic solvent and extracted, a concentrate obtained by filtration, reduced pressure and concentration can be used. Alternatively, it is possible to use a soaking agent, a pre-preparation, a clove and a fluid extract produced by drying the concentrate again. The submerged product can be submerged using citrus peel or green tea solvent through any method such as cooling, percolation, digestion, etc. to obtain a submerged product containing an active ingredient. The organic solvent may be one or more selected from C _{1 to} C ₄ anhydrous or hydrous alcohols, acetone, ethyl acetate, chloroform, glycerin, propylene glycol, and butylene glycol. It is not limited.

  Meanwhile, in one embodiment of the present invention, the composition may be for preventing skin erythema or improving skin color. In one embodiment of the present invention, the composition may be used to prevent skin aging, increase skin elasticity, improve skin wrinkles, improve blood color, shrink pores, alleviate skin irritation, improve homeostasis, slimming, atopy, antibacterial, allergy, elasticity. In addition to acne, regeneration, sebum, waste products, keratin improving effect, skin convergence, and organizing effect, it may be used for improving obesity and diabetes, but is not limited thereto.

  The skin external preparation composition according to the present invention is a concept that generally encompasses all the compositions used for external skin use, such as a cosmetic composition and a pharmaceutical composition.

  Cosmetic compositions include, for example, basic cosmetics, makeup cosmetics, hair cosmetics, body cosmetics, etc. The dosage form is not particularly limited and is appropriately selected depending on the intended purpose. can do.

  For example, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, It may be formulated into a spray or the like, but is not limited thereto. In more detail, soft lotion, nourishing lotion, lotion, body lotion, nourishing cream, massage cream, moisture cream, hand cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, gel, patch, underwater Basic cosmetics such as oil (O / W) type, water-in-oil (W / O) type, color cosmetics such as lipstick, makeup base or foundation, shampoo, rinse, body cleanser, toothpaste or oral cleanser It may be formulated into a cosmetic composition for hair such as a hair styling agent such as a cleaning agent, hair tonic, gel or mousse, a hair nourishing agent or a hair dyeing agent.

  The cosmetic composition contains a cosmetically acceptable medium or base. This is all dosage forms suitable for topical application, eg solutions, gels, solids or kneaded anhydrous products, emulsions obtained by dispersing the oil phase in the aqueous phase, suspensions, microemulsions, microcapsules In the form of fine granulocytes or ionic (liposomes) and / or non-ionic vesicle dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conceal sticks. These compositions are prepared by conventional methods in the art.

  In the present invention, when the dosage form is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, There are benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

  In the present invention, when the dosage form is a suspension, the carrier component is water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, or polyoxyethylene. Suspending agents such as sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant are used.

  In the present invention, when the dosage form is a paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, parapine, starch, tracant, cellulose derivative, polyethylene glycol, silicon, bentonite, Silica, talc or zinc oxide is used.

  In the present invention, when the dosage form is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, the dosage form is a spray. In some cases, it may further include a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether.

  In the present invention, when the dosage form is cleansing containing a surfactant, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoesteral, isethionic acid, imidazolinium derivatives, Methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamide betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester are used. .

  In one embodiment of the present invention, the cosmetic composition may further contain a thickener. As the thickener contained in the cosmetic composition of the present invention, methylcellulose, carboxymethylcellulose, carboxymethylhydroxyguanine, hydroxymethylcellulose, hydroxyethylcellulose, carboxyvinyl polymer, polyquaternium, cetearyl alcohol, stearic acid, carrageenan and the like are used. Also good. Preferably, one or more of carboxymethylcellulose, carboxyvinyl polymer, and polyquaternium may be used, and most preferably carboxyvinyl polymer may be used.

  In one embodiment of the present invention, the cosmetic composition may contain various appropriate bases and additives as required, and the types and amounts of these components are easily selected by the inventor. be able to. If necessary, it may contain an acceptable additive. For example, it may further contain components such as preservatives, dyes, additives and the like which are usual in the art. More specifically, the preservative may be phenoxyethanol or 1,2-hexanediol, and the pigment may be an artificial fragrance.

  In one embodiment of the present invention, the cosmetic composition may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts. . Other ingredients that can be added include oil and fat ingredients, moisturizers, emollients, surfactants, organic or inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts , PH adjusters, alcohols, pigments, fragrances, blood circulation promoters, cooling sensates, antiperspirants, purified water, and the like.

  Moreover, the compounding component which can be added other than that is not limited to this, Moreover, all the said components can be mix | blended in the range which does not impair the objective and effect of this invention.

  In one embodiment of the present invention, the pharmaceutical composition comprises a tangerine peel extract containing tangeretin and a green tea extract containing EGCG as active ingredients, and a conventional inorganic or organic carrier is added to form a solid, semi-solid, It can be formulated into a parenteral administration agent in a solid or liquid form. The preparation for parenteral administration may be a transdermal dosage form selected from the group consisting of drops, ointments, lotions, gels, creams, patches, sprays, suspensions and oils. However, it is not limited to this.

  In the external preparation composition for skin according to each dosage form, other components other than the above-described composition of the present invention are appropriately selected and blended without difficulty by those skilled in the art according to the dosage form of other external preparation for skin or the purpose of use. In this case, a synergistic effect may occur when applied simultaneously with other ingredients.

  In addition, when the composition according to the present invention is used as a pharmaceutical composition, a pharmaceutical adjuvant such as a preservative, a stabilizer, a hydrating agent or an oiling accelerator, a salt or a buffer for adjusting osmotic pressure, Alternatively, it can further contain other therapeutically useful substances and can be formulated into various oral or parenteral dosage forms by conventional methods.

  The actual dosage of the active ingredient should be understood as to be determined by various relevant factors such as the severity of the symptoms, the chosen route of administration, the age, sex, weight and health status of the subject.

  In general, the dosage of active ingredient ranges from about 0.001 mg / kg / day to 2000 mg / kg / day. A more preferable dose is 0.5 mg / kg / day to 2.5 mg / kg / day.

  Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and dosage form examples. However, these test examples and dosage form examples are provided for illustrative purposes to assist in understanding the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Reference Example 1] Production of mandarin peel extract containing tangeretin The mandarin peel is washed with purified water and then dried. The citrus peel from which moisture has been removed is crushed and the active ingredients are extracted through a cold extraction method at room temperature for 40 to 48 hours. The extracted contents are first filtered with a 250 μm mesh, and then the upper layer liquid is taken through a centrifugation process. The contents are further filtered sequentially with 3 μm, 1 μm, and 0.5 μm mesh filter paper. Add 1,3-butylene glycol (Butylene glycol) 10%, phenoxyethanol 0.5%, ethylhexylglycerol (Ethylhexylglycerin) 0.05%, and filter with 0.2-0.3 μm filter paper. A mandarin peel extract is obtained.

[Reference Example 2] Production of Green Tea Extract Containing EGCG After washing tea leaves with purified water, it undergoes a drying process. The tea leaves from which moisture has been removed are crushed and the active ingredients are extracted through a cold extraction method at room temperature for 40 to 48 hours. The extracted contents are first filtered with a 250 μm mesh, and then the upper layer liquid is taken through a centrifugation process. The contents are further filtered sequentially with 3 μm, 1 μm, and 0.5 μm mesh filter paper. Add 1,3-butylene glycol 10%, phenoxyethanol 0.5%, ethylhexylglycerin 0.05%, and further filter through 0.2-0.3 μm filter paper to obtain a green tea extract.

[Test Example 1] Inhibitory effect on melanogenesis HM3KO cells of human melanoma cells (Y. Funasaka, Department of dermatology, KOBE university school of medicine, 501 Kusunoki-cho 7-chloro 7 It is placed in MEM (Minimum Essential Medium) containing 10% fetal bovine serum and cultured under conditions of 37 ° C. and 5% CO ₂ . Place the cells cultured in this way in 75 flasks so that the number of cells is 3 x 10 ⁵ per flask, and wait for the cells to attach to the wall for one night. After that, the medium is replaced with a fresh medium containing 10 ppm of each test substance shown in Table 1 below. At this time, the control group used a medium not added, and used both citrus peel extract and green tea extract as Example 1, Kojic acid known to show a whitening effect as Comparative Example 1, citrus The skin extract is referred to as Comparative Example 2, and the green tea extract is referred to as Comparative Example 3. Incubate until the cells fill the flask, replacing with fresh medium containing the sample once every 2-3 days. After the cells finished growing, they were collected and compared with the color of the cells of the control group, Example 1, and Comparative Examples 1 to 3, respectively. Further, after removing the culture medium and washing with PBS, after dissolving in 1N sodium hydroxide and measuring the absorbance at 500 nm, the melanin production inhibition rate was calculated by the following mathematical formula 1, and the results are shown in Table 1 below. It was shown to.

  From the results of Table 1, when Example 1 using both mandarin orange extract and green tea extract is used, the inhibition rate of melanin production similar to kojic acid, which is known to have a very good whitening effect, is obtained. It turns out to have. Moreover, compared with the comparative example 2 and the comparative example 3 which respectively used the mandarin orange peel extract and the green tea extract, the very outstanding melanin production suppression rate which reached 4 to 5 times was shown. This is because the melanin production inhibitory effect is increased by the synergistic effect of tangeretin contained in the citrus peel extract and EGCG contained in the green tea extract.

[Production Example 1 and Comparative Production Examples 1 to 4]
Cosmetic compositions of Production Example 1 and Comparative Production Examples 1 to 4 were produced with the compositions described in Table 2 below. When the manufacturing process is examined in detail, the raw materials 1 to 9 are mixed and dissolved at 70 ° C. to form an aqueous phase part, while the raw materials 10 to 15 are dissolved at 70 ° C. to form an oil part. The oil part was added to the aqueous phase part and stirred with a homomixer (Nippon Tokushu Kika) to perform primary emulsification, and then the raw materials 16 and 17 were added to increase the viscosity. After removing the bubbles, the mixture was cooled at room temperature to produce cosmetics of Production Example 1 and Comparative Production Examples 1 to 4 (unit: mass%).

[Test Example 2] Whitening effect on human skin In order to examine the whitening effect and skin irritation on human skin, the upper arm region of a subject was examined for 30 healthy men and women with an average age of 35.2 years old. After adhering an opaque tape with 6 holes of 1.5 cm in diameter, the sample was irradiated with ultraviolet rays (UVB) of about 1.5 to 2 times the minimum erythema dose of each subject. Skin darkening was induced, the test substance was applied, and two months later, the lightness of the skin was measured using a color difference meter. These subjects were allowed to apply the cosmetics of Production Example 1 and Comparative Production Examples 1 to 4 twice daily in the morning and evening.

  The effect was determined by determining the value of “L” indicating the brightness of the skin. As a reference, the skin color of Koreans not artificially baked generally shows a value of 50-70. Using a color difference meter (Minolta CR2002), the black and white level of the skin was measured to determine the effect. In order to display the color, the L * a * b * color system is used. In the present invention, L * value (lightness) is mainly used as an index. The L * value was calibrated with a standard white plate, and the measurement was repeated five times or more at one site, thereby uniformly measuring the pigmented portion. The difference in skin color (ΔL *) between the application start time and the completion time was calculated by the following mathematical formula 2, which is shown in Table 3 below. The whitening effect is determined by comparing ΔL * of the sample application site and the control group site. If the ΔL * value is about 2, it is determined that the whitening of the deposited pigment is clear. If it is about 5 or more, it can be determined that there is a whitening effect.

  When the test substance is applied and effective, the L value will increase sequentially, and the test substance is compared with the difference in skin color (ΔL) between the start of application and the end of application (two months after the start of application). Was calculated by the mathematical formula 2 and the results are shown in Table 3 below.

[Test Example 3] Investigation of skin irritation to human skin After using the cosmetics of Production Example 1 and Comparative Production Examples 1 to 4 for the subject of Test Example 2 for two months, The skin irritation was compared. At this time, the judgment standard is that the subject gave a score of 1 to 5 according to the degree of irritation and compared, meaning that there is no stimulation as it is closer to 1, and there is more stimulation as it is closer to 5, The average score for each is shown in Table 3 below.

  As can be seen from the results in Table 3, the cosmetic of Production Example 1 using a mixture of citrus peel extract and green tea extract according to the present invention has a whitening effect better than Comparative Production Example 1. At the same time, it was confirmed that the skin irritation had a similar value as when kojic acid was used in the same amount. Moreover, the whitening effect of the manufacture example 1 which mixed and used the orange extract of the mandarin orange and the green tea extract is also the outstanding whitening effect of about 4 to 5 times more than the cosmetics of the comparative manufacture examples 3 and 4. It was found that the skin irritation feeling was also greatly reduced. This is determined to be due to a synergistic effect of mixing tangeretin contained in the citrus peel extract and EGCG contained in the green tea extract.

[Test Example 4] Change in angular mass In order to examine the effect of reducing the angular mass of the whitening cosmetic composition according to the present invention, the change in skin angular mass of the cosmetics produced from Production Example 1 and Comparative Production Examples 1 to 4 was examined. I tried.
After applying the cosmetic compositions of Production Example 1 and Comparative Production Examples 1 to 50 on the lower arm of 50 adult men and women in their 20s and 30s who have no skin disease, 24 hours have passed and Charm view (Charm view) The amount of keratin loss was measured using Moritex, Japan). Prior to the start of application, the initial skin angular mass was measured using a charm view under constant temperature and humidity conditions (24 ° C., humidity 40%) to obtain a basic value, and the change after 24 hours was measured. The results are shown in Table 4 below.

  From the results of Table 4, it can be seen that Production Example 1 of the whitening cosmetic composition according to the present invention is superior in skin keratin reduction effect as compared with Comparative Production Examples 1 to 4. Moreover, in the case of the manufacture example 1 manufactured by mixing the tangerine peel extract and the green tea extract, it can be seen that the synergistic effect has the most excellent skin keratin reduction effect.

  Therefore, it was confirmed that the composition further comprising EGCG in tangeretin according to the present invention can provide excellent skin safety by minimizing skin irritation, as well as excellent skin whitening effect.

[Test Example 5] Evaluation of free radical removal ability: DPPH assay
In order to confirm the free radical scavenging ability by the mixed use of tangeretin and EGCG using DPPH (2,2-Diphenyl-1-picrylhydrazyl, Sigma, D9132), DPPH assay was performed. When DPPH, which has a purple color, reacts with an antioxidant substance, it receives hydrogen electrons from the antioxidant substance, changes its color, and the absorbance at 517 nm decreases.

First, DPPH is prepared. Before starting the experiment, it is dissolved in ethanol to make 100 μM, sonication is performed and completely dissolved, and then the treatment substances are prepared in the following order.
1) Dilute a 10% tangeretin solution [solvent: dimethyl sulfoxide (DMSO)] in ethanol according to the treatment concentration.
2) The number of treatments with the same concentration is set to 3 for each treatment concentration interval of the substance.
3) When reacting with DPPH solution, the concentration is reduced to 1/20, so prepare a substance 20 times the concentration to be confirmed.

  Thereafter, 10 μL of the treatment substance is placed in a 96-well plate for each treatment concentration. In the positive control group, 10 μL of vitamin C is added at 2.5, 5, and 10 ppm concentrations. The control group contains 10 μL of ethanol. Thereafter, 190 μL of 100 μM DPPH is added, and if the substance has color, the absorbance can be affected. Therefore, an experimental group in which 190 μL of ethanol is added to 10 μL of substance is also prepared.

  The substance reaction is stored for 30 minutes in an incubator at 37 ° C., and then the absorbance at 517 nm is measured using a spectrophotometer (Spectra Max 190). Concentration change when green tea extract containing EGCG is mixed with green tea extract containing vitamin C and EGCG, mandarin peel extract containing tangeretin, and mandarin peel extract containing tangeretin The absorbance value (OD) was measured by the method shown in FIG.

  The greater the antioxidant power of the substance, the lower the absorbance value. The concentration (IC50: 50% Inhibitory activity) of the substance that reduces the absorbance value in half compared with the control group was determined. It can be determined that the smaller the IC50 value, the greater the antioxidant power.

  In FIG. 1, “*” indicates a case where a mandarin peel extract (−) containing tangeretin and a green tea extract containing EGCG are mixed with a mandarin peel extract containing tangeretin ( +) Indicates that there is a “significant difference” and the p-value calculated from the experimental results is less than 0.05, which is considered to have reliable significance. . According to the results of FIG. 1, it is confirmed that there is an antioxidant effect as a significant difference when treating both EGCG and tangeretin at all concentrations as compared to when treating both alone. I was able to.

Moreover, the photograph in FIG. 1 represents the color change in DPPH assay of Test Example 5. When the purple DPPH reacts with an antioxidant substance, it receives hydrogen electrons from the antioxidant substance and changes its color. It was found that when the two were treated together, the dark purple color of the control group was significantly lighter than when EGCG and tangeretin were treated alone.
Therefore, it was confirmed that not only the whitening effect through the antioxidant synergy effect of tangeretin and EGCG but also the prevention of skin oxidation and vitality of the skin.

  Examples of dosage forms of the cosmetic composition and pharmaceutical composition according to the present invention will be described below. However, the present invention can be applied to various dosage forms other than those exemplified below, and this is intended to limit the present invention. It is not intended to be described, but merely for concrete explanation.

[Form example 1] Soft lotion (skin lotion)
According to the composition described in Table 5 below, a soft lotion was produced by an ordinary method.

[Form example 2] Nutrition lotion (milk lotion)
With the composition described in Table 6 below, a nutritive lotion was produced by a conventional method.

[Dosage Form Example 3] Nutrition Cream Nutrition cream was produced by the usual method according to the composition described in Table 7 below.

[Formulation Example 4] Massage Cream A massage cream was produced by the usual method according to the composition described in Table 8 below.

[Dosage Form Example 5] Pack A pack was produced by the usual method according to the composition described in Table 9 below.

[Formulation Example 6] Drug for local administration (patch)
A patch was prepared by the usual method according to the composition described in Table 10 below.

  A person having ordinary knowledge in the field to which the present invention belongs can make various applications and modifications within the scope of the present invention based on the above contents.

  In the above, specific portions of the present invention have been described in detail. For those having ordinary skill in the art, the specific technique is merely a preferred implementation example, and the scope of the present invention is not limited thereby. Is clear. Accordingly, the substantial scope of the present invention is defined by the appended claims and equivalents thereof.

Claims (6)

  A skin external preparation composition comprising both tangeretin and EGCG.   2. The skin external preparation composition according to claim 1, wherein the tangeretin is contained in a citrus peel extract.   The skin external preparation composition according to claim 1 or 2, wherein the EGCG is contained in a green tea extract.   The skin external preparation composition according to claim 2, wherein the mandarin orange extract is contained in an amount of 0.001 to 40 mass% with respect to the total mass of the composition.   The skin external preparation composition according to claim 3, wherein the green tea extract is contained in an amount of 0.001 to 40% by mass with respect to the total mass of the composition.   The composition for external use of skin according to claim 1 or 2, wherein the composition is for skin whitening.