JP2001122765A - Active oxygen scavenger and cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain both an active oxygen scavenger having safety and stability, capable of scavenging active oxygen to cause various agings and diseases and an aging preventing cosmetic by making the cosmetic include the active oxygen scavenger and to develop a way applicable to a wide technology field of medicine/food as well. SOLUTION: The active oxygen scavenger and the cosmetic comprises a plant extract having an active oxygen scavenging action admitted in an extract obtained by extracting any of various plants with water, an aqueous solution of a lower alcohol and/or the lower alcohol as an active ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, an antioxidant and a cosmetic containing these as an active ingredient. In particular, the cosmetic according to the present invention is mainly intended to provide an anti-aging cosmetic such as skin, but the field of application of the present invention is not limited to the technical field of cosmetics, and It can be widely applied to various technical fields such as food and food.

[0002]

2. Description of the Related Art Humans use oxygen as energy.
You. As long as oxygen is used as energy, some
Oxygen is hydrogen peroxide (H₂O₂) 、 Super oxide
(O₂ ⁻ ), Hydroxyl radical (OH⁻) And other active acids
This active oxygen causes various aging and diseases.
It has been proposed that And the negative effects of active oxygen
To remove superoxide dimutase (SO
It is possible to directly eliminate active oxygen with an enzyme such as D)
it can.

[0003] Since human skin is located in the outermost layer of a living body, it is constantly exposed to oxidative stress due to endogenous active oxygen or exogenous active oxygen generated by ultraviolet rays. Mucopolysaccharides represented by hyaluronic acid, a major component in the dermis of the skin, play an important role in keeping the skin firm. Mucopolysaccharides are said to be fragmented by active oxygen and ultraviolet rays, leading to aging of the skin. Therefore, suppressing fragmentation of mucopolysaccharides present in the dermis of the skin is important for skin aging.

[0004] Furthermore, the sebum secreted on the skin is useful for protecting the skin from exogenous stress, but the sebum is oxidized by ultraviolet rays and the like to lipid peroxide, and the lipid peroxide is oxidized to the skin. In addition to being irritating, it is said to be involved in aging by attacking and damaging cells and causing various other adverse effects. Therefore, suppressing the production of lipid peroxide is considered to be effective not only in preventing deterioration of the skin (skin) state but also in preventing aging of the living body itself.

[0005]

DISCLOSURE OF THE INVENTION The present invention relates to fragmentation of mucopolysaccharide, which is a main component of skin, in order to search for extracts having various effects on skin aging from various plant extracts. Searching for those that have an inhibitory effect;
To search for substances that have an active oxygen (direct) scavenging action that causes adverse effects such as aging, etc., substances indirectly generated by the presence of active oxygen (such as lipid peroxides)
As a result of research for the purpose of searching for those that prevent adverse effects such as aging, respectively, those having a mucopolysaccharide fragmentation inhibitory action and effect from the above,
The present invention has been completed based on the finding of a substance having a more active oxygen scavenging action and a substance having a more antioxidant effect.

Therefore, the present invention provides a stable and safe mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, an antioxidant, and the like, which are free from side effects obtained from a group of extracts from natural products (plants). Cosmetics using these (anti-aging cosmetics)
The active ingredients of the mucopolysaccharide fragmentation inhibitor, active oxygen scavenger and antioxidant according to the present invention are not only in the technical field of cosmetics but also in the technical field of pharmaceuticals and foods. It will open up the way of using it widely in the technical field.

[0007]

Means for Solving the Problems To achieve the above object, the present invention provides a method comprising the following steps: Ryutan, Kikyo, Hibiscus, Aloe, Rhubarb, Yellow sperm, Uwaurushi, Prolonged life grass, Sanshishi, Yangmei skin, Kuzuru, Psycho, Senkyu, Light load Leaves,
Licorice, peonies, peony, midsummer, toki, cinnamon, yellow orchid, peony, gentian, juniper, five times child, assemblage, gennoshoco, mahuang, yellow cedar, ougon, cinnabar, turmeric, goshyu, sage, oregano rosemary, Laurel, tarragon, onion, nutmeg, clove, wasabi, sabori, basil, chili, beans tea, black tea,
Various plant extracts extracted with water or lower alcohol or a lower alcohol aqueous solution from each plant consisting of green tea, persimmon leaves, coffee, sugina, bee, wormwood, kumasasa, wolfberry, japonica, shiitake mushroom, and L-cysteine , Glutathione, mannitol, gallic acid, potassium sorbate, acetylacetone, triethanolamine, tannic acid, 4-tert-butyl-4'-methoxy-dibenzomethane, 2-hydroxy-4-methylbenzophenone-5-sulfate A mucopolysaccharide fragmentation inhibitor comprising one or more selected from one or both of the compound groups as an active ingredient.

[0008] Hibiscus, aloe, rhubarb, yellow sperm, Uwaurushi, prolonged life grass, Sanshishi, Yangmei skin, Kakkon, Psycho, Senkyu, Soujutsu, Thin leaf, Bukuroyou,
Licorice, peonies, spicy, midsummer, toki, cinnamon, jujube, oren, peony, gentian, quintoko, assembly, gennoshoko, mahuang, huangkashia, apricot, ginger, tissou, ogon, cinnamon, turmeric, shintou, Kojitsu, ground yellow, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, onion, nutmeg, mace, clove, wasabi, sabori, basil, pepper, bean tea, black tea, green tea, persimmon leaf, coffee, Too much, the bee,
Wormwood, kumasasa, wolfberry, jasotetsu, shiitake mushroom, ginkgo, hijiki, igisu, kombu, arame, oniwakame,
Any one of a group of various plant extracts extracted from each plant comprising water or a lower alcohol or a lower alcohol aqueous solution, and a compound group consisting of tannic acid and 2-hydroxy-4-methylbenzophenone-5-sulfate Or one or two selected from both groups
An active oxygen scavenger comprising at least one species as an active ingredient is constituted.

[0009] Hibiscus, aloe, rhubarb, yellow sperm, Uwaurushi, prolonged life grass, Yangmei plum, Kakkon, Sengyu,
Soujutsu, thin leaves, licorice, peonies, yokuinin,
Xinyi, cinnamon bark, jujube, oren, peony, gentian, quince, cassava, gennoshoko, huangkashi, ginger, ogong, borei, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, nutmeg, Mace, clove, wasabi, sabori, basil, pepper, bean tea, black tea, green tea, persimmon leaf, coffee, sugina, bee, wormwood, amachazur, kumasasa, wolfberry, jassotetsu, shiitake mushroom, hijiki, seaweed, eggplant, kombu Containing, as an active ingredient, one or two or more selected from various plant extracts extracted from water or lower alcohol or a lower alcohol aqueous solution from each plant consisting of: Constitute an antioxidant.

As an active ingredient, one or more selected from the group consisting of the above-mentioned mucopolysaccharide fragmentation inhibitor, the above-mentioned active oxygen scavenger and the above-mentioned antioxidant is contained. This constitutes a cosmetic characterized by the above. Accordingly, each invention is constituted. Next, each invention will be described in detail.

The "mucopolysaccharide fragmentation inhibitor", the "active oxygen scavenger", the "antioxidant" and the "cosmetic" according to the present invention are one or both of various plant extracts and various compounds. One or more selected from the above are contained as active ingredients. The various extracts of the plant refer to extracts extracted from various plants with water, lower alcohol or an aqueous solution of lower alcohol. As used herein, the term "lower alcohol" means methanol, ethanol, or propanol, and refers to alcohols that can be mixed with water at any ratio.

As the test plants used in the examples of this specification, dried and powdered plants were used. In addition, extracts of plants with lower alcohols referred to in the examples of this specification were obtained using a Soxhlet extractor. Extracts of water or a lower alcohol aqueous solution were obtained by leaching and extracting a predetermined amount of a sample with a predetermined amount of water or a lower alcohol aqueous solution under predetermined conditions. For various plant extracts, the organic solvent is distilled off for the lower alcohol extract, and the water extract is freeze-dried as it is, and for the lower alcohol extract, the lower alcohol of the extract is used. After concentrating under reduced pressure, the mixture was freeze-dried to obtain each plant extract.

First, the "mucopolysaccharide fragmentation inhibitor" according to the present invention will be described. The term "mucopolysaccharide" as used in the present invention refers to a concept including all mucopolysaccharides of neutral mucopolysaccharide and acidic mucopolysaccharide, and particularly human mucopolysaccharides, that is, hyaluronic acid, chondroitin, chondroitin 4-sulfate. , Chondroitin 6-sulphate, dermatan sulphate, heparan sulphate, peppera and keratan sulphate I and keratan sulphate II.

The "mucopolysaccharide fragmentation inhibitor" according to the present invention has industrial utility from the following viewpoints. That is, mucopolysaccharides (such as hyaluronic acid), which are the main components in the dermis of human skin, are regarded as important components for maintaining skin firmness. It is considered that this mucopolysaccharide loses its original function by being fragmented and reduced in molecular weight by ultraviolet rays or active oxygen generated in a living body. Therefore, preventing fragmentation of this mucopolysaccharide (such as hyaluronic acid) is considered to be effective for preventing wrinkles on human skin (skin) and preventing aging of the skin. Furthermore,
The "mucopolysaccharide fragmentation inhibitor" according to the present invention will be used in the pharmaceutical and food industries.

Formulation of various active ingredients (one or more selected from one or both of various plant extracts and various compounds) in the "mucopolysaccharide fragmentation inhibitor" according to the present invention. The amount (content) varies depending on the kind of the active ingredient and the combination thereof, the purpose of use, the embodiment, the form of use, the number of uses, and the like.
There is no particular limitation as it can be performed. In principle, it is sufficient that an effective amount is present, but generally it is 0.0001 to 100 based on the composition of the mucopolysaccharide fragmentation inhibitor.
%, Preferably 1 to 10% by weight is available. Furthermore, the active ingredient (various plant extracts and various compounds) according to the present invention can exert its function and effect even if it is one kind. However, when two or more kinds of active ingredients are appropriately used in combination, an excellent effect is obtained. A synergistic effect can be achieved. Naturally, the active ingredient according to the present invention (one or more selected from one or both of various plant extracts and various compounds) may be used in combination with a known “mucopolysaccharide fragmentation inhibitor”. By doing so, an excellent synergistic effect can be achieved.

The mucopolysaccharide fragmentation inhibitory activity of various plant extracts and various compounds according to the present invention was measured by a known method. That is, by combining the fragmentation reaction with active oxygen (ascorbic acid-iron system) and the complex formation reaction between albumin and mucopolysaccharide, the fragmentation reaction of mucopolysaccharide by ultraviolet light (UV-A) and By combining the complex formation reaction with mucopolysaccharide, each was measured and searched for the presence or absence of each active ingredient. Table 1 shows the measurement results of the water extract of each plant. Table 2 shows the measurement results of the lower alcohol aqueous solution (50% ethanol aqueous solution) extract.
Shows the measurement results of the lower alcohol (99.9% ethanol) extract, respectively.

The present inventors have determined that the "mucopolysaccharide fragmentation inhibitor" according to the present invention may be applied to a plant extract or compound having a fragmentation inhibition rate of 20% or more in Tables 1, 2 and 3. It was determined that the compound was a plant extract and a compound that could be used. That is, as a group of plant extracts, Ryutan, Kikyo, Hibiscus, Aloe, Rhubarb,
Yellow sperm, uwaurushi, life-prolonging grass, sanshishi, Yangmei-peel, kakkon,
Psycho, Sengoku, Light-Loaded Leaf, Bulk Leopard, Licorice, Peony, Yokuinin, Shinyi, Midsummer, Toki, Chrysanthemum, Yellow Lian,
Peony skin, gentian, juniper, five times child, assemblage, genoshoko, mahuang, huangbashi, ougon, chen, turmeric,
Goshyu, sage, oregano, rosemary, laurel, tarragon, onion, nutmeg, cloves, wasabi, sabori, basil, pepper, bean tea, black tea, green tea, persimmon leaf, coffee, sugana, honey, wormwood, kumasasa, wolfberry One or two or more species selected from a group of plant extracts consisting of various extracts of various plants extracted with water or a lower alcohol or an aqueous solution of a lower alcohol from each plant consisting of A. cylindrica and Shiitake mushroom Newly found out. On the other hand, compounds include L-cysteine, glutathione, mannitol, gallic acid, potassium sorbate, acetylacetone, triethanolamine, tannic acid, 4-tert-butyl-4'-methoxy-
1 selected from the group consisting of dibenzomethane, 2-hydroxy-4-methylbenzophenone-5-sulfate
It has been newly found that any species or two or more compounds can be used. Therefore, the mucopolysaccharide fragmentation inhibitor according to the present invention is effective at least one or more selected from either one or both of the group consisting of the plant extract group and the compound group. It is a mucopolysaccharide fragmentation inhibitor characterized by containing as a component.

Next, the "active oxygen scavenger" according to the present invention will be described. The "active oxygen scavenger" according to the present invention has industrial applicability from the following viewpoints. That is, it is known that active oxygen generated in a living body causes aging and disease. In vivo, it can be eliminated by enzymes such as superoxide mutase (SOD). However, it is considered that the ability to scavenge such active oxygen declines with aging and accelerates aging and disease.
Further, the superoxide mutase (SOD) is a high-molecular protein, and its activity is quickly deactivated, which is not practical. On the other hand, the active oxygen scavenger according to the present invention is present in various extracts of each plant, and has an extremely excellent active oxygen scavenging ability and has stable characteristics. In particular, a crude drug is a complex, and has a remarkable property that it acts on various types of active oxygen in a well-balanced manner and has excellent stability against heat and the like. Furthermore, the "active oxygen scavenger" according to the present invention will find use in the pharmaceutical and food industries.

In the "active oxygen scavenger" according to the present invention, the content (content of one or more selected from one or both of various extracts of plants and various compounds) of the active ingredient is contained. The amount of the active ingredient, the combination thereof, and the purpose of use, the embodiment,
There is no particular limitation because it can be varied according to the use form, the number of uses, and the like. In principle, it is sufficient that an effective amount is present, but generally 0.0001 to 100% by weight, preferably 1 to 10% by weight, based on the active oxygen scavenger composition can be used. Furthermore, the active ingredient (various plant extracts and various compounds) according to the present invention
Can exert an effect even if only one kind is used, but an excellent synergistic effect can be achieved by appropriately combining and using two or more kinds of active ingredients. Of course,
The active ingredient according to the present invention (one or more selected from any one or both of various plant extracts and various compounds) is excellent in synergy when used in combination with a known active oxygen scavenger. An effect can also be achieved.

The measurement of the active oxygen scavenging action of various plant extracts and various compounds according to the present invention was performed by a known method. That is, a phosphate buffer containing xanthine and nitroblue tetrazolium is reacted with xanthine oxidase under predetermined conditions, the reaction is stopped with sodium dodecyl sulfate, and the absorbance at 560 nm is measured to search for the presence or absence of each active ingredient. Was. In addition, a specific measuring method and a method of calculating the active oxygen suppression rate will be described in detail in Examples.

Table 4 shows the results obtained by measuring the active enzyme elimination rate (%) for various extracts and compounds of the plant according to the present invention. The inventors have determined that those having an active oxygen scavenging rate of 20% or more in Table 4 can be used as the "active oxygen scavenger" according to the present invention. That is, as various plant extracts, hibiscus, aloe, rhubarb, yellow sperm, awaurushi, prolonged life grass, sanshishi,
Yang plum bark, kakkon, psycho, senkyu, sojutsu, thin leaves, bukuro, licorice, peonies, spicy, midsummer, toki, cinnamon, jujube, oren, peony, gentian, quintet,
Assembling, Gennoshoko, Mao, Huangbashi, Almond, Ginger,
Taisou, Orgon, Chen-skin, Turmeric, Shinobi, Kyojitsu, Chihoku, Garlic, Sage, Oregano, Rosemary, Laurel, Celery, Thyme, Taragon, Onion,
Nut Meg, Mace, Clove, Wasabi, Sabori, Basil, Chili, Bean Tea, Black Tea, Green Tea, Persimmon Leaves, Coffee, Sugi, Hachik, Wormwood, Kumasasa, Kuko, Jabsotetsu,
Selected from a variety of plant extracts consisting of various extracts of water or lower alcohols or aqueous solutions of lower alcohols from each plant consisting of shiitake mushroom, ginkgo, hijiki, japonica, kombu, arashi, and squid It has been newly found that one or more plant extracts can be used. On the other hand, as the compound, tannic acid, 2-hydroxy-4-methylbenzophenone-5-
It has been newly found that one or both of the compounds composed of sulfate can be used. Therefore, the "active oxygen scavenger" according to the present invention is one or more selected from one or both of the plant extract group and the compound group that can be used as an active oxygen scavenger. Is an active oxygen scavenger characterized by containing as an active ingredient.

Further, the "antioxidant" according to the present invention will be described. "Antioxidant" according to the present invention
Is considered to be of industrial value from the following perspectives. That is, sebum is secreted on human skin in order to protect the skin (skin). This sebum is oxidized by ultraviolet rays or the like to become lipid peroxide, and this lipid peroxide irritates the skin. In addition, the lipid peroxide attacks cell membranes to cause damage and various other adverse effects, and it is said that these disorders further contribute to aging of human skin. Therefore, it is considered that suppressing the production of the lipid peroxide is effective not only for preventing deterioration of the skin (skin) condition but also for preventing aging of a living body. Furthermore, the "antioxidant" according to the present invention will be used in the pharmaceutical and food industries.

The amount (content) of various active ingredients (various plant extracts) in the "antioxidant" according to the present invention.
Is not particularly limited because it can be varied according to the kind of the active ingredient and the combination thereof, the purpose of use, the embodiment, the form of use, the number of uses, and the like. In principle, an effective amount may be present, but generally 0.0001 to 100% by weight, preferably 1 to 10% by weight, based on the antioxidant composition can be used. Furthermore, the active ingredient (various plant extracts) according to the present invention can exert its function and effect even by one kind, but two or more kinds of effective ingredients (various plant extracts) can be used in appropriate combination. More excellent synergistic effects can be achieved. Of course, the active ingredient (various plant extracts) according to the present invention can also exhibit an excellent synergistic effect when used in combination with a known “antioxidant”.

The antioxidant activity of the various plant extracts and various test compounds according to the present invention was measured by a known method (a rodan iron method utilizing autoxidation of linoleic acid). Then, various extracts and various compounds of each plant were searched for the presence or absence of each active ingredient. In addition, a specific measuring method and a method of calculating the antioxidant activity rate will be described in detail in Examples.

Table 5 shows the results of measuring the antioxidant rate (%) of various extracts and compounds of the plant according to the present invention. The inventors found that the antioxidant rate in Table 5 was 20%.
% Was determined to be usable as the "antioxidant" according to the present invention. That is, as various plant extracts, hibiscus, aloe, rhubarb, yellow sperm, awaurushi, prolonged life grass, Yangmei skin, kakkon, Sengyu,
Soujutsu, thin leaves, licorice, peonies, yokuinin,
Xinyi, cinnamon bark, jujube, oren, peony, gentian, quince, cassava, gennoshoko, huangkashi, ginger, ogong, borei, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, nutmeg, Mace, clove, wasabi, sabori, basil, pepper, bean tea, black tea, green tea, persimmon leaf, coffee, sugina, bee, wormwood, amachazur, kumasasa, wolfberry, jassotetsu, shiitake mushroom, hijiki, seaweed, eggplant, kombu It is possible to use, as an active ingredient, one or more selected from various plant extracts extracted from water or a lower alcohol or a lower alcohol aqueous solution from each plant consisting of squid, seaweed, seaweed and blue seaweed. Newly found out. Therefore, the antioxidant according to the present invention is an antioxidant characterized by containing, as an active ingredient, one or more selected from the various plant extract groups.

The "antioxidant" according to the present invention can be applied not only to cosmetics but also to various related technical fields such as various drugs, various drugs, chemicals and fertilizers. In addition, the active ingredients according to the present invention are all excellent in that they have low toxicity and irritation to the skin, high stability to heat and light, and high stability to various cosmetic bases and cosmetic additives. Has characteristics.

Furthermore, the "cosmetic" according to the present invention
Will be described. The "cosmetic" according to the present invention is considered to have industrial value from the following viewpoints.
That is, as described above, “mucopolysaccharide fragmentation inhibitor”,
Since it is composed of a cosmetic containing one or more of the active oxygen scavenger and the antioxidant as active ingredients,
The actions and effects that can be achieved based on the types of various plant extracts and the types of compounds to be incorporated into the cosmetic can be directly used as the actions and effects of the cosmetic. Therefore, the cosmetic according to the present invention can provide a skin (skin) anti-aging cosmetic as a basic action and effect.

The amounts (contents) of the various active ingredients (various plant extracts and compounds) according to the present invention with respect to various cosmetics are determined by the types and combinations of the active ingredients and the types and cosmetics of the cosmetics. Purpose, embodiment,
It is not particularly limited because it can be changed according to the use form and the number of times of use of the cosmetic. In principle, it is sufficient that an effective amount be present, but generally 0.0001 to 100% by weight of the cosmetic composition can be used, preferably 0.01 to 10% by weight, and especially 0.1 to 10% by weight. 1-5.0% by weight is optimal. In particular, when applied to cosmetics as a cosmetic composition to be prepared at the time of use, any one selected from the mucopolysaccharide fragmentation inhibitor, active oxygen scavenger and antioxidant according to the present invention or 2 or more is 10
It will be used at a high blending ratio including 0% by weight.

Furthermore, the active ingredient (various plant extracts and compounds) according to the present invention can exert its effect even if it is selected from the group consisting of various plant extracts and compounds. An excellent synergistic effect can be obtained by appropriately combining and using two or more kinds of active ingredients. Naturally, the active ingredients (various plant extracts and various compounds) according to the present invention include known "mucopolysaccharide fragmentation inhibitors" and "active oxygen scavengers".
It goes without saying that an excellent synergistic effect can also be achieved by using in combination with one or more selected from among "antioxidants".

The application range of the cosmetic according to the present invention is not particularly limited. In other words, the present invention can be applied to all cosmetics that can utilize the respective effects according to the effects of the active ingredient of the present invention. For example, one or more of the active ingredients according to the present invention are blended with various cosmetic bases and the like, and various basic cosmetics such as creams, emulsions, lotions, packs, face wash, foundations, blushers, etc. , Lipstick, white powder, etc., various makeup products, hair styling, hair restorer, shampoo, rinse, etc., various hair cosmetics, soap, beautiful nails,
Widely applicable to other cosmetics such as perfume, cologne, etc. Further, embodiments of the various cosmetics,
Solutions, emulsions, ointments, oils, waxes, sols,
It can be applied in various forms such as gel, powder (powder) and spray (aerosol).

The various extracts of each plant according to the present invention include:
Since all of them are composites, they have excellent properties that they act on various types of active oxygen in a well-balanced manner, have good thermal stability, and can provide highly safe cosmetics.

[0032]

The various extracts and compounds of the plant according to the present invention are highly safe and stable on the basis of their excellent active oxygen-suppressing action on mucopolysaccharide fragmentation and ultraviolet light suppressing mucopolysaccharide fragmentation. "Mucopolysaccharide fragmentation inhibitor" can be provided, and thus the skin (skin)
Aging prevention can be achieved.

Further, the various extracts and compounds of the plant according to the present invention are highly safe and stable based on their excellent active oxygen scavenging action.
Can be provided, whereby aging prevention and disease prevention can be achieved. In particular, a remarkable action and effect can be achieved in preventing aging of the skin (skin).

Further, the various extracts and compounds of the plant according to the present invention suppress the formation of substances (for example, lipid peroxides) which are adversely affected by active oxygen due to their excellent antioxidant action, and are safe. It can provide a high and stable “antioxidant”, so that the skin (skin)
Can be prevented, and the aging of the living body can be prevented.

[0035]

Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. In addition, the part in an Example shows a weight part unless there is particular notice.

(1) Preparation of plant extract. As a raw material of the plant extract, 10 g of a dried and powdered product of each plant was used. First, for the preparation of the "water extract of a plant", 10 g of the above-mentioned raw material is put into a thimble filter paper,
It was immersed in 100 ml of ion-exchanged water, and heated and extracted at 60 ° C. for 8 hours to obtain a filtrate. This operation was repeated four times, and all the filtrates were combined and freeze-dried to obtain a water extract (dry powder) of the plant. Next, ethanol was used as the lower alcohol for the preparation of “lower alcohol extract of plant” and “extract of lower alcohol aqueous solution of plant”. Second
Regarding the preparation of “a 50% ethanol aqueous solution extract of a plant”, a 50% ethanol aqueous solution was used instead of water in the extraction operation in the preparation of the water extract.
Then, extraction was performed under reflux conditions during the extraction operation. All extracts were combined, and the concentrated extract after distilling off ethanol as much as possible was lyophilized to give "50 plants".
% Ethanol aqueous solution extract ". Third, regarding the preparation of "ethanol extract of plant", after extracting for 8 hours using a Soxhlet extractor, the solvent is distilled off, and the extract is powdered to obtain "ethanol extract of plant". Was.

(2) Reagents. As a sample of mucopolysaccharide, sodium hyaluronate (microbial origin) (manufactured by Meiji Seika Co., Ltd.) was used. On the other hand, as a positive target of the substance inhibiting the fragmentation of hyaluronic acid by ultraviolet rays, 4-t-butyl-4′-methoxy-dibenzomethane (hereinafter referred to as “Parsol 1789”) (GI
VADAN) and 2-hydroxy-4-methylbenzophenone-5-sulfate (hereinafter, referred to as "ASL-24S") (manufactured by Shonan Chemical Industry Co., Ltd.).

(3) Search test for mucopolysaccharide fragmentation inhibitory action. As a sample of mucopolysaccharide, sodium hyaluronate (commercial product) was used. As plant extracts, extracts from 77 types of plants, such as crude drugs, spices, and tea, were searched. In addition, a search test for the mucopolysaccharide fragmentation inhibitory action was similarly performed for 11 kinds of compounds.

(A) Active oxygen (ascorbic acid-iron system)
A method for measuring the inhibitory effect on the fragmentation of hyaluronic acid. To 0.45 ml of a 0.3 M phosphate buffer (pH 5.3) containing 0.04% sodium hyaluronate, 0.05 ml of a 1% aqueous sample solution (an aqueous solution of various plant extracts or a compound) and ascorbin After adding 0.025 ml of acid and 0.025 ml of 1 mM aqueous ferric chloride solution and incubating at 37 ° C. for 24 hours, 0.2 ml of the reaction solution was taken out, and 0.04 M sodium acetate containing 0.1% albumin was added thereto. 2.0 ml of /0.08 M acetate buffer (pH 3.75) is added and stirred well. 5
After standing for a minute, the turbidity of the formed complex of hyaluronic acid and albumin is measured as the absorbance at 600 nm (Esr). (Amount of residual hyaluronic acid)

In the measurement of the amount of hyaluronic acid used in the present method,
Since there is a possibility that a complex may be formed between a plant extract or the like and albumin, the turbidity, that is, the absorbance (Eb) when only sodium hyaluronate was removed in the above-mentioned measurement operation was corrected as a blank.

The original amount of hyaluronic acid used in the sample was determined by measuring the turbidity of the complex with albumin at 600 nm when the fragmentation operation of hyaluronic acid in the ascorbic acid-iron system was omitted from the above measurement operation. Absorbance (Eso)
Was measured by

The fragmentation inhibitory activity rate (%) of hyaluronic acid is calculated by calculating the amount of hyaluronic acid obtained by measuring the fragmentation of hyaluronic acid with the ascorbic acid-iron system according to the above-mentioned method [“residual hyaluronic acid amount (Esr)”]. The ratio (%) to the original amount of hyaluronic acid [“the original amount of hyaluronic acid (Eso)”] used in the sample was calculated and obtained by [Equation 1].

[0043]

(Equation 1)

(B) A method for measuring the inhibitory effect on fragmentation of hyaluronic acid by ultraviolet light (UV-A). 1 ml of 0.3 M phosphate buffer (pH 5.3) containing 0.04% sodium hyaluronate was dispensed into each well of a 12-well multi-dish (22 mmφ), and 1% of a sample aqueous solution (various extraction of plants) 0.1 ml of an aqueous solution of a substance and a compound soluble in water) to form a hyaluronic acid layer. On the other hand, on the hyaluronic acid layer, 2-ethylhexanoic acid setosteryl alcohol ester was added in an amount of 0.5 m.
Layer 1 and dissolve the oil-soluble one. Then, ultraviolet rays were irradiated at 37 ° C. for 7 days using an ultraviolet lamp (Dermalei FL20SBLB / manufactured by Toshiba Medical Products Co., Ltd.). The wavelength of the ultraviolet light and the total amount of irradiation energy for 7 days for this irradiation were 365 nm (12.0 KJ / cm ² ) and 306 nm.
nm (67.0 J / cm ² ).

0.2 ml of the aqueous layer solution was taken out from each well, and 2.0 ml of 0.04 M sodium phosphate / 0.08 M acetate buffer (pH 3.75) containing 0.1% albumin was added thereto. Stir well. After standing for 5 minutes, the turbidity of the formed complex of hyaluronic acid and albumin was reduced to 60%.
It was measured as absorbance at 0 nm (Esr). (Amount of Residual Hyaluronic Acid) All tests were performed aseptically, and the hyaluronic acid solution was sterilized by an autoclave. The lid of the 12-hole multi-dish was taken, covered with a plastic bag, and irradiated with ultraviolet rays.

In the measurement of the amount of hyaluronic acid used in this method,
Since there is a possibility that a complex may be formed between a plant extract or the like and albumin, turbidity, that is, absorbance (Eb) when only sodium hyaluronate was removed in the above method as a blank was measured and corrected.

The original amount of hyaluronic acid used in the sample was determined by measuring the turbidity of the complex with albumin at 600 nm when the fragmentation operation of hyaluronic acid in the UV-A system was omitted from the measurement operation. It was measured as absorbance (Eso). (Original amount of hyaluronic acid)

The calculation of the rate of inhibition of fragmentation of hyaluronic acid by the ultraviolet system (%) is carried out in the same manner as in the case of the calculation of the rate of suppression of fragmentation of hyaluronic acid by the ascorbic acid-iron system in (A). The amount of hyaluronic acid ("residual amount of hyaluronic acid (Esr)") of the original test hyaluronic acid ("original test") used for the sample of the amount of hyaluronic acid ("residual amount of hyaluronic acid (Esr)") for which fragmentation of hyaluronic acid was measured by ultraviolet rays (UV-A system) Hyaluronic acid content (Es
o)) and the ratio (%) to () was calculated by [Equation 2].

[0049]

(Equation 2)

Table 1 shows the results of the water extract of each plant and the inhibitory effect of each compound on hyaluronic acid fragmentation. [Table 2] shows the 50% aqueous ethanol extract of each plant and the results of the hyaluronic acid fragmentation inhibitory action of each compound.
[Table 3] shows the results of the ethanol extract of each plant and the hyaluronic acid fragmentation inhibitory effect of each compound.

In Tables 1 to 3, "ASA-Fe system" in the column of fragmentation method refers to the measurement of the inhibitory effect on the fragmentation of hyaluronic acid by active oxygen (ascorbic acid-iron system). The results (hyaluronic acid fragmentation inhibition rate) measured by the method are shown.
The results (inhibition rate of hyaluronic acid fragmentation) measured by a method for measuring the inhibitory effect on hyaluronic acid fragmentation by VA) are shown. In addition, the numbers in the table all indicate the suppression rate (%).

[0052]

[Table 1]

[0053]

[Table 2]

[0054]

[Table 3]

From these results, water extracts of various plants,
Mucopolysaccharide fragmentation inhibitory effects are observed in the aqueous ethanol extract and various extracts and compounds of the ethanol extract.

Retrieval test for active oxygen scavenging action. (4) Method for measuring active oxygen scavenging action. First, the following color reagent, enzyme solution, blank solution and reaction stop solution are prepared. Coloring reagent: Xanthine is dissolved in a 0.1 M phosphate buffer (pH 8.0) at a concentration of 0.04 mmol / l, and nitro blue tetralithium (NO ₂ -TB) is dissolved at a concentration of 0.24 mmol / l. Enzyme solution: 0.1 M phosphate buffer (pH 8.0) and xanthine oxidase (Buttermilk / Buttermatter)
(from ilk) is dissolved at 0.049 units / ml. Blank solution: 0.1 M phosphate buffer (pH 8.0) Reaction stop solution: 69 mM sodium dodecyl sulfate

The "enzyme / sample sample (S)" was prepared by adding 1.0 ml of a 1% aqueous sample solution (an aqueous solution of various extracts or compounds of a plant) to 1.0 ml of a color developing solution and 1.0 ml of an enzyme solution.
After heating at 37 ° C. for exactly 20 minutes, the reaction is stopped by adding 2.0 ml of a reaction stopping solution. After stopping the reaction,
The absorbance (Es) at 560 nm is measured. Also,
"Enzyme sample (B)" is 0.1% of distilled water instead of 1% of the sample (aqueous solution of various extracts or compounds of plant).
1 and the same operation as in the case of the above-mentioned "enzyme / sample sample" measurement was performed, and the absorbance at 560 nm (E
b) is measured.

On the other hand, the "enzyme-free sample sample (SB)" was prepared by adding 1.0 ml of a color developing reagent solution and 1.0 ml of a blank solution to 0.1 ml of a 1% aqueous sample solution (aqueous solution of various extracts or compounds of plants). After heating at 37 ° C. for exactly 20 minutes, the reaction was stopped by adding 2.0 ml of a reaction stop solution.
The absorbance at 60 nm (Esb) is measured. Also,
The “enzyme-free sample (BB)” was prepared by adding 0.1% distilled water instead of a 1% sample (aqueous solution of various extracts or compounds of plants).
1 ml is added thereto, and the same operation as in the above “non-enzyme / sample sample” is performed, and the absorbance (Ebb) at 560 nm is measured. Then, the active oxygen elimination rate (%) was calculated by the following [Equation 3].

[0059]

(Equation 3)

Table 4 shows the active oxygen scavenging rate (%) of each sample thus measured.

[0061]

[Table 4]

From Table 4, it can be seen that various extracts and compounds of the plant according to the present invention have excellent active oxygen scavenging action.

Search test for antioxidant activity. (5) Method of measuring antioxidant action (by the iron-rodan method). 0.2 ml of 0.01% sample solution (plant extract solution or compound solution) is added to 1 ml of 2 × 10 ⁻² M linoleic acid stock solution and 0.2 ml of 0.1% sample solution.
0.8 ml of 1 M phosphate buffer (pH 7.0) was added,
The mixture was sealed and reacted at 37 ° C. for 48 hours.
The amount of peroxide was measured by the rhodane iron method. 0.1 ml of the mixed solution after the reaction was taken out, and 4.7 ml of 75% ethanol and 0.3% of 30% ammonium thiocyanate solution were added thereto.
1 ml, and stirred. Further, 0.1 ml of a 3.5% hydrochloric acid solution containing 2 × 10 ⁻² M ferrous chloride was added, followed by stirring.
The absorbance (As) at 00 nm was measured. As a negative control, 500 n when distilled water was used in place of the 0.01% sample solution (plant extract solution or compound solution)
The absorbance (Ac) at m was measured. In addition, 500 nm when a 0.01% emix solution (Tween 20 was added and dissolved in water) was added as a positive control.
The absorbance (Ab) at is measured. Then, the antioxidant activity (%) is calculated by [Equation 4].

[0064]

(Equation 4)

Table 5 shows the antioxidant activity (%) of each of the various extracts and compounds of the plant according to the present invention.

[0066]

[Table 5]

From the results shown in Table 5, various extracts and compounds of plants have antioxidant activity.

Next, examples (formulation examples) of cosmetics using various extracts of plants according to the present invention will be shown. According to this embodiment, the cosmetic according to the present invention is not limited at all.

(6-1) Cream (W / O type) Oily component: (wt%) Plant extract ……………………………… 0.1 Cetanol… …………………… jojoba oil ……………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………. ……………………… 37.5 Beeswax ………………………………………… 6.0 Emulsifier: Lipophilic Glycerin monostearate ... 2.0 Polyoxyethylene sorbitan monolaurate (20.EO.) ........................... 2.0 Perfume ………………………………… Appropriate amount of preservative ………………………………………………………………………………… Aqueous phase: Dipropylene gly …………………… 2.0 Glycerin ………………………………… 3.0 Purified Water ………… 30.0 [Production method] The components of the aqueous phase are mixed, heated and maintained at 70 ° C to obtain an aqueous phase. On the other hand, other components are mixed and dissolved by heating to 70 ° C. to obtain an oil phase. This oil phase is added to the above-mentioned aqueous phase to perform preliminary emulsification, uniformly emulsified by a homomixer, and cooled to 30 ° C. to obtain a cream of the product.

(6-2) Emulsion Oil phase (wt%) Plant extract ……………………………… 0.1 Cetanol ……………… ………………………………………………………………………………………………………………… 10.0 10.0 Cetyl octanoate ……………………… 10.0 emulsifier: sorbitan sesquioleate 3.0 polyoxyethylene hydrogenated castor oil (50 EO) 3.0 perfume …………………………… Appropriate amount Preservative …………………………………… Appropriate amount Aqueous phase: Glycerin …………………………… 3.0 Purified water ……………………………………………………… 65.0 [Production method] Mix the components of the aqueous phase, add And keeping water phase portion 70 ° C. to. On the other hand, the other components were mixed and dissolved by heating.
Set the oil phase to ° C. This oil phase is added to the above-mentioned aqueous phase and emulsified, and cooled to 30 ° C. to obtain an emulsion of the product.

(6-3) Creamy Foundation Oil Phase: (wt%) Plant Extract ………………………………… 0.2 Stearic Acid ………… ………………………………………………………………………………………………………………………………………………………………………………………………… 2.5. ............ 1.0 Propylene glycol monolaurate ............ 3.0 Squalane ............ ............ ...... 7.0 Cetyl octanoate ………………… 8.0 Water phase: Purified water …………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………… 53 .3 Triethanolamine 1.2 Sorbit .................. Preservatives 3.0 Preservatives ………………………………………………………………… ...... ……………………………… Appropriate amount Pigment: Titanium oxide …………………………… 8.0 Kaolin …… ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………. …………………… 1.0 Colored pigment ……………………………………… Appropriate amount of perfume ………… ……………………………………… Appropriate amount [Production method] Pigment is mixed and ground. An aqueous phase is prepared, and the mixed pigment is added and dispersed therein, and then heated to 75 ° C.
Prepare oil phase and heat to 80 ° C. The oil phase is added to the aqueous phase with stirring, emulsified and then cooled, the fragrance is added at 50 ° C, and further cooled to 30 ° C to obtain the product.

(6-4) Lipstick (wt%) Base: plant extract ……………………………… 0.2 Castor oil …………… ……………… 45.0 Hexadecyl alcohol …………………… 25.0 Beeswax ……………………………… ………… 5.0 Candelilla Row ………………………… 7.0 Carnauba Row ………………………………… ...... 6.0 Lanolin ........................................................ 4.0 Coloring material: Titanium oxide ........................ ……… 2.0 Coloring ………………………………… Appropriate amount of fragrance ……………………………………… Appropriate amount [Production method] Heat and melt the base material and uniformly Zell. The coloring material is added to the mixture, and the mixture is kneaded with a roll mill to uniformly disperse the coloring material.

(6-5) Solid Foundation Pigment: (wt%) Titanium oxide ……………………… 13.0 Kaolin ……………. ……………… 25.0 Talc …………………………………… 45.0 Bengala ……………………………. ………………………………………………………………………………………………………………………………………… 2.5 Black iron oxide ………… …………………………………… 0.1 Binder: plant extract ……………………………………………………………………………………………………………………………… 0.1 0.1 Squalane… ………………………………………… Sorbitan sesquioleate ……………………………… 3.5 Preservatives ………………………………… ……………………………… Appropriate amount of fragrance ……………… ............................................. appropriate amount of Manufacturing Method] pigments are mixed and milled by a pulverizer. This is transferred to a high speed blender where the binder and preservative are mixed and the homogenized is added with pigment to make it more uniform. This is processed by a crusher and compression molded.

(6-6) Powder-shaped Pack Powder: (wt%) Kaolin …………………. Talc ………………………… 20.0 Aene oxide ………………………… 19.0 Oil : Olive oil 2.0 Dispersant: Polyoxyethylene sorbitan monolaurate (40 EO) ... ... ... ... ... 1.0 Moisturizer: Glycerin 8.0 Preservatives: Ethyl paraben Ethyl paraben ……………………… ………… Appropriate amount of plant extract …………………………… 10.0

[0075]

EFFECT OF THE INVENTION Based on the properties of mucopolysaccharide fragmentation inhibitory action, active oxygen scavenging action and antioxidant action possessed by various plant extracts and compounds, various aging and diseases caused by active oxygen can be effectively prevented. It is possible to provide a mucopolysaccharide fragmentation inhibitor, an active oxygen scavenger, and an antioxidant which can be prevented and are excellent based on these properties. Moreover, when the mucopolysaccharide fragmentation inhibitor, active oxygen scavenger and antioxidant are derived from various plant extracts, the plant contains a large amount of crude drugs and the like. Achieved the purpose of the invention, as it is a natural product and a compound, it is safe, stable to heat, etc., has few side effects, and has widespread applications not only in cosmetics but also in the technical fields of medicine and food. Has a remarkable effect.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61K 35/78 A61K 35/78 QJNFBK HHR A UT 35/80 35/80 Z 35 / 84 35/84 A A61P 17/00 A61P 17/00 39/06 39/06 (72) Inventor Hiroshi Tanaka 1-11-17 Ebie, Fukushima-ku, Osaka-shi, Osaka Naris Cosmetics Co., Ltd. (72) Inventor Atsushi Shiba 1-11-17 Ebie, Fukushima-ku, Osaka-shi, Osaka, Japan

Claims (2)

[Claims] 1. Hibiscus, aloe, rhubarb, yellow sperm, uwaurushi, life-prolonging grass, sanshishi, Yangmei-ki, kakkon, psycho, senkyu, suzujutsu, light-leafed leaves, bukuro,
Licorice, peonies, spicy, midsummer, toki, cinnamon, jujube, oren, peony, gentian, quintoko, assembly, gennoshoko, mahuang, huangkashia, apricot, ginger, tissou, ogon, cinnamon, turmeric, shintou, Kojitsu, ground yellow, garlic, sage, oregano, rosemary, laurel, celery, thyme, tarragon, onion, nutmeg, mace, clove, wasabi, sabori, basil, pepper, bean tea, black tea, green tea, persimmon leaf, coffee, Too much, the bee,
Wormwood, kumasasa, wolfberry, jasotetsu, shiitake mushroom, ginkgo, hijiki, igisu, kombu, arame, oniwakame,
Any one of a group of various plant extracts extracted from each plant comprising water or a lower alcohol or a lower alcohol aqueous solution, and a compound group consisting of tannic acid and 2-hydroxy-4-methylbenzophenone-5-sulfate Or one or two selected from both groups
An active oxygen scavenger comprising at least one species as an active ingredient. 2. A cosmetic comprising the active oxygen scavenger according to claim 1 as an active ingredient.