JPH05320063A - Active oxygen-inhibiting agent - Google Patents

Abstract

PURPOSE:To provide the active oxygen-inhibiting agent originated from a plant, capable of being applied to skins, having good safety even when applied as a medicine or food, and exhibiting an active oxygen-inhibiting effect. CONSTITUTION:This active oxygen-inhibiting agent contains the solvent extract of at least one kind selected from the dry root and stem of Dryopteris crassirhizoma Nakai (utilized as an antifebrile, antidote, homeostatic agent, insecticide), the dry cortex, seed skin and root skin of Punica granatum L. (utilized as an anthelmintic, gargle), the dry fruit of Areca catechu L. (utilized as a stomachic, astringent, anthelmintic), the dry root of Berchemia lineata DC. (utilized for pulmonary tuberculosis, lung cancer, bruise, distortion) and the dry stem of Ephedra sinca Stapf (utilized as a diaphoretic, antitussive, expectorant). Water or a hydrophilic organic solvent is used as the extraction solvent, and especially water, ethanol or their mixture is preferable. The inhibiting agent inhibits the production of peroxides in a body, prevents the damages of phospholipids in biomembranes, prevents the injury of DNA, retards the aging of man, and furthermore inhibits the melanism of skins.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention is a plant-derived active oxygen inhibitor which is applied to cosmetics, pharmaceuticals and foods as an active oxygen inhibitor.

[0002]

2. Description of the Related Art Ganshu is a member of the phylum Phytophyta, Foliar nets, Subcapsular follicles, and Osters of the family Osteridae (Lepidoptera: Pyralidae).
Crassirhizoma Nakai) and other rhizomes are dried. It is used as an antipyretic, detoxifying, hemostasis, insecticide.

Stone bark, stone gall bark, stone gall root bark are dicotyledonous nets, nymphs, pomegranates, pomegranate genus Punica granatum
L.) dried bark or fruit bark or root bark. Pomegranate is a deciduous tree native to Asia Minor and cultivated for medicinal purposes or appreciation in Japan during the Heian period. It is used as a parasite control and mouthwash.

Coleoptera is a dicotyledonous plant, palm order, palm family, areca of the genus Jinjuju, scientific name Areca catechu
Dried fruit of El (Areca catechu L.).
Areca are distributed in southern China, Taiwan, and Malaysia, and are cultivated in tropical regions. Used as a stomachic, digestive, astringent, anthelmintic drug for indigestion, constipation, abdominal pain, and tapeworm control.

Iron encasement is a dicotyledonous plant, a drowsiness eye,
Black-buckthorn family, the genus Patricia, scientific name Berchemia lineata
lineata DC.) roots are dried. Himekumayanagi is a medicinal shrub that is distributed in the subtropical regions of Himalayas, India, Indochina, southern China, and Taiwan. In Japan, it grows on Amami Oshima and Ryukyu reefs. It is used for pulmonary tuberculosis, lung cancer, bruising, and sprains.

Mao is a zoophyte, maou net, maou eye,
The aerial stalk of Ephedra sinica Stapf, whose scientific name is Ephedra sinica Stapf, is dried. Maou is native to Mongolia and northeastern China, and is an evergreen small shrub that grows in sandy areas. It is used as a perspiration, antitussive, expectorant, in respiratory distress due to impaired excretion function of skin and mucous membranes, asthma, chills, body pain, and phalangeal pain.

On the other hand, various substances are known as substances having an active oxygen suppressing effect that can be used as raw materials for cosmetics, pharmaceuticals and foods, but synthetic products are safe when they are applied to human skin for a long period of time. There is no guarantee of the use, and the use is being restricted. On the other hand, many natural products have a weak effect of suppressing active oxygen. However, from the viewpoint of safety for human skin, it is a natural product,
For many years, there has been a demand for a substance that is eaten by humans and is guaranteed in terms of safety, has a strong effect of suppressing active oxygen, and has other effects on humans and skin.

[0008]

SUMMARY OF THE INVENTION An object of the present invention is to provide an active oxygen inhibitor containing a component that is safe for application to the skin and used internally as a medicine or food and has an active oxygen inhibitory effect. That is.

[0009]

[Means for Solving the Problems] In order to solve the above problems, the present inventors have screened and investigated plants that have been used internally as pharmaceuticals for many years and have been confirmed to be safe for the human body, and cosmetics. , Considered useful as pharmaceuticals and foods. As a result, it was found that the solvent extracts of ginseng, stone gall bark, stone glazed bark, stone gall root bark, cabbage ginger, iron encasement, and mahuang are very effective as active oxygen inhibitors.

That is, the present invention relates to an active oxygen inhibitor containing at least one solvent extract selected from the group consisting of ginseng, stone gall bark, gypsum flesh, stone gall bark, calyx, iron metal, and mahuang. Is.

[0011] As a method of utilizing the ginseng, stone gall bark, stone glazed skin, stone glazed root bark, cabbage, iron metallurgy and mahuang, extraction is carried out with water or a hydrophilic organic solvent such as ethanol, methanol or acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable because it is extraction of cosmetic raw materials. Also, in some cases,
A polyhydric alcohol such as glycerin, 1,3 butylene glycol or propylene glycol or a mixed liquid of polyhydric alcohol and water can also be used for extraction. It is also effective to freeze-dry and use it as a powder depending on the method of use.

This substance may be used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, moisturizers such as glycerin and 1,3 butylene glycol, and various agents. It can be added to prepare cosmetics in various dosage forms. For example, a lotion, a cream, a milky lotion, a pack or the like may be used depending on the purpose.

There is oxygen in the air, and without it, no organisms (except anaerobic ones) can exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids and produces peroxides. It also oxidizes and damages the phospholipids of biological membranes in the body. In addition, the generated peroxide and active oxygen damage DNA. It is said to promote aging. Furthermore, it is said that the generated peroxide and active oxygen damage DNA and promote aging. This active oxygen also affects the mechanism of melanin production from tyrosine and is also involved in blackening the skin. Suppressing this active oxygen is an important factor for the skin, in other words, an important factor required for cosmetics.

[0014]

EXAMPLE An example which is an actual method of use will be described below, but the present invention is not limited to this example. The production examples of the extract of ginseng, stone gall bark, stone gall bark, stone gall root bark, cabbage ginger, iron metallurgy and mahuang used in the present invention are shown below. (Example 1) 10 g of ginseng (dried product) and 30 g of methanol
0 ml was added and left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Embodiment 2) The total amount of dried product is 5 g per 10 g.
300 ml of 0% aqueous methanol solution was added and the mixture was left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Example 3) 10 g of gypsum bark (dry product)
300 ml of methanol was added to and the mixture was left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Example 4) 10 g of stone gall bark (dry product)
300 ml of 50% aqueous methanol solution was added to the above and left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Example 5) 10 g of stone gall bark (dry product)
300 ml of purified water is added to and heated for 3 hours. This was allowed to cool, filtered, and then freeze-dried.

(Embodiment 6) 10 g of stone glazed skin (dry product)
Ethanol (300 ml) was added to the above and left for 5 days with occasional stirring. This was filtered and then frozen.

(Embodiment 7) 10 g of stone glazed skin (dry product)
Add 300 ml of ethanol aqueous solution to the mixture and stir 5 occasionally.
Left for days. This was filtered and then frozen.

(Embodiment 8) 10 g of stone glazed rind (dried product)
300 ml of purified water is added to and heated for 3 hours. This was allowed to cool and then filtered and freeze-dried.

(Example 9) 10 g of stone garlic root bark (dry product)
300 ml of methanol was added to the above and left for 5 days while occasionally drying. This was filtered and freeze-dried.

(Example 10) 10 stones of garlic root bark (dried product)
300 ml of 50% methanol aqueous solution was added to g and left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Embodiment 11) 10 stones of garlic root bark (dried product)
Add 300 ml of purified water to g and heat for 3 hours. This was allowed to cool, filtered, and then freeze-dried.

(Embodiment 12) 10 g of garlic radix (dried product)
300 ml of methanol was added to and the mixture was left for 5 days with occasional stirring. This was filtered and then frozen.

(Embodiment 13) 10 g of Reiko (dried product)
300 ml of 50% aqueous methanol solution was added to the above and left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Embodiment 14) 10 g of 榳 榔 子 (dry product)
300 ml of purified water is added to and heated for 3 hours. This was allowed to cool, filtered, and then freeze-dried.

(Example 15) 10 g of iron envelop (dry product)
300 ml of methanol was added to and the mixture was left for 5 days with occasional stirring. This was filtered and then frozen.

(Example 16) 10 g of iron packaging (dry product)
300 ml of 50% aqueous methanol solution was added to the above and left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Example 17) 10 g of iron envelop (dry product)
300 ml of purified water is added to and heated for 3 hours. This was allowed to cool, filtered, and then freeze-dried.

Example 18 To 10 g of mahuang (dry product) was added 300 ml of methanol, and the mixture was left for 5 days with occasional stirring. This was filtered and then frozen.

Example 19 To 10 g of mahuang (dry product) was added 300 ml of 50% methanol aqueous solution, and the mixture was left for 5 days with occasional stirring. This was filtered and freeze-dried.

(Example 20) 300 g of purified water was added to 10 g of a dried product and heated for 3 hours. This was allowed to cool, filtered, and then freeze-dried.

(Formulation Example-1) Lotion Olive oil 0.5 Methanol extract of Ginseng of Example 1 0.5 Polyoxyethylene (20E.O.) Sorbitan monostearate 2.0 Polyoxyethylene (60E.O) .) Hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

(Formulation Example-2) Cream A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 50% Methanol Extract of Example 2 1.0 B Purified Water 47.9 Polyoxyethylene (20 E.O.) Sorbitan Monostearate 2.0 Polyoxyethylene (60 E.O.) Curing Castor oil 1.0 Glycerin 5.0 1.0% sodium hyaluronate aqueous solution 5.0 Methyl paraoxybenzoate 0.1 Weigh A and B respectively, heat to 70 ° C., gradually stir B while stirring A After adding to, mix slowly with stirring
Cooled to ° C.

(Effect of Active Oxygen Suppression Test) There are various methods for measuring the effect of suppressing active oxygen, but the following method was used this time. pH 7.8 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml Catalase above potassium phosphate buffer 5 ml 2 mM Nitroblue tetrazolium above potassium phosphate buffer 5 ml 1.8 mM xanthine above phosphorus Potassium acid buffer 17 ml 160 ml of the above reagent mixture of 2.4 ml and 0.3 ml of sample were added to xanthine oxynase (use water as the sample in advance, so that the absorbance will increase by about 0.02 per minute. Adjust with the above potassium phosphate buffer) 0.3
Immediately after adding ml, the absorbance (560 nm) is measured. (Measurement is performed in two quantiles to confirm linearity) Calculation formula Inhibition rate = ((A−B) / A) × 100 A: change in absorbance per minute when the sample is water B: sample Change in Absorbance Per Minute Several concentration steps were performed to search for a 50% active oxygen production inhibitory concentration. The preparation method of the sample is that the above production example (freeze-dried product) is dissolved in an aqueous solution of appropriate concentration (if it is difficult to dissolve, ethanol is added and then purified water is added, and then evaporated.
After removing ethanol, the concentration was adjusted to an appropriate concentration). Table 1 shows the results of the extract of each Example, expressed as a 50% active oxygen production inhibitory concentration.

[0037]

[Table 1]

[0038]

EFFECTS OF THE INVENTION Each of the examples is a result of an extract of a single substance such as ginseng, stone bark and the like. However, since the measurement characteristics have the same active oxygen suppressing effect, a mixture of two or more of them is obtained. Have also been confirmed to have an additive effect, and therefore a mixture of two or more kinds thereof also has a similar active oxygen suppressing effect.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masao Hattori 2556-4-2 2-203 Gofuku Suehiro-cho, Toyama City, Toyama Prefecture (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Takamatsu 1207-3 Kakihana-cho, Matsusaka City, Mie Prefecture, Koori

Claims (1)

[Claims] Claims: 1. Ganshu, stone gall bark, gypsum flesh, gem root bark,
An active oxygen inhibitor containing at least one solvent extract selected from the group consisting of Renjako, iron-clad metal, and mahuang.