JPH07138126A - Antioxidant - Google Patents

Abstract

PURPOSE:To provide an antioxidant safe to human body, exhibiting excellent antioxidant action and effective for preventing the chapped skin and keeping the springiness and gloss of the skin. CONSTITUTION:This antioxidant contains at least one kind of solvent extract of plants selected from among Nigella sativa, Munronnia pumila, Borrelia hispida, Phyllanthus nuriri, Evolvus alsinoides, Sphaeranthus indicus, Murraya Koenigii, Traguia involclata and Ocimum sanctum.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antioxidant having a high antioxidant effect and effective against rough skin.

[0002]

2. Description of the Related Art Nigera sativa is a plant of the family Ranunculaceae, and its seeds are used externally and internally for anthelmintic, carminative, or lung disease, cough, and jaundice.

Munronia pumilla is a plant of the genus Meliaceae, which grows naturally in the lowland rocks of Sri Lanka, India. The decoction of this plant is used as an excellent stomach medicine and for heat, dysentery and blood purification.

Borrelia hispida is a plant of the Rubiaceae family, distributed in India, Sri Lanka, the Malay Peninsula, China, Java Island and the Philippine Islands, and is a common plant in lowland and dry wastelands. It is used as a vegetable, as a medicine for the treatment of hemorrhoids, and as a seed for diarrhea and dysentery.

[0005] Phyllanthus nuriri is a plant of the dicotyledonous net, the isolated leaflet sub net, Euphorbiaceae, and is widely distributed in the tropical region. In Sri Lanka, it is distributed as a weed in wastelands and cultivated lands. In Sri Lanka, it is used for diarrhea, jaundice and gonorrhea.

[0006] Morning glory (Evolvulus alsinoide)
s) is a bindweed plant that grows well in the dry grasslands of the provinces of India, Sri Lanka, the Philippine Islands and Zhejiang, Jiangxi, Fujian, Taiwan, Guangdong, Guangxi, Sichuan and Yunnan in China. Also known as Tsucho Katsura. It is used for jaundice, diarrhea, and skin diseases.

Spheransus indicus is a plant of the Asteraceae family, which includes India, Sri Lanka, Malaysia, Africa,
Commonly found in damp areas of lowland rice fields in China, roots and seeds are used as anthelmintic drugs, bark is used as a remedy for hemorrhoids, and whole plants are used as detoxifying fish.

[0008] Muraya Coenigy is a dicotyledonous net, a leaflet sub net, a dwarf carnivorous plant of the family Rutaceae, distributed in India and Sri Lanka, and is commonly found in arid lowlands. As a drug, it is used for constipation, abdominal cramps, diarrhea, etc.

[0009] Tolugia involucurata is a plant of the dicotyledonous net, the isolated leaf subnet, and Euphorbiaceae, and is distributed in India, Sri Lanka, Myanmar, and China. In Sri Lanka, it is well distributed in lowland wastelands. In India, sweating, laryngitis,
It is used for bronchitis, pneumonia and leprosy.

Ocimum sanctum is a plant of the Labiatae family that is often found in India and Sri Lanka. It is an important plant in Hindu and its leaves are malaria.
The root is used as a medicine for dysentery and indigestion.

On the other hand, various substances are known as substances which can be used as a raw material of cosmetics and have an antioxidant action,
Synthetic products do not guarantee safety when applied to human skin for a long period of time, and their use is being limited. On the other hand, many natural products have weak antioxidant effects. However, it is a natural product from the viewpoint of safety for human skin, has been guaranteed for safety for many years to be eaten by humans and used as a medicine, and has a strong antioxidant action, and also has other effects on the skin. It was desired to have a material that had it together.

[0012]

SUMMARY OF THE INVENTION An object of the present invention is to provide an antioxidant which is safe when applied to the skin, has a large antioxidant effect, and contains a component effective for rough skin and the like.

[0013]

[Means for Solving the Problems] In order to solve the above-mentioned problems, the present inventors have screened and investigated plants that have been used for food for many years and have been confirmed to be safe for the human body, and have investigated antioxidants. I examined what is useful as. As a result, they have found that the following solvent extracts of plants are very effective as an antioxidant raw material or as a quasi drug.

That is, the present invention is applicable to Nigera sativa, Munronia pumilla, Borrelia hispida, and Firansus.
At least one selected from the group consisting of Nuriri, Morning glory, Spheranthus indyx, Muraya Coenigy, Torgia involucata, and Kamimeboki
Antioxidants including solvent extracts of seeds.

As a method of utilizing these plants, water or a hydrophilic organic solvent such as ethanol, methanol,
Extract with acetone. However, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable because it is the extraction of the antioxidant raw material for cosmetics.

In some cases, glycerin, 1,
A polyhydric alcohol such as 3-butylene glycol or propylene glycol or a mixed liquid of polyhydric alcohol and water can also be used for extraction. Further, freeze-drying and using it as powder is also effective depending on the method of use.

This substance may be used as other cosmetic raw materials such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various agents. It can be added to prepare various dosage forms of antioxidants. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

[0018]

EXAMPLE An example of an actual usage will be described below, but the present invention is not limited to this example. An example of producing a solvent extract of a plant used in the present invention is shown below.

(Production Example 1) 300 g of ethanol was added to 10 g of Nigella sativa seeds (dry product), and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 2) To 10 g of Nigella sativa seeds (dried product), 300 ml of 50% ethanol aqueous solution was added and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 3) To 10 g of Nigella sativa seeds (dried product), 300 ml of purified water is added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 4) 300 ml of ethanol was added to 10 g of whole grass (dry product) of Munronia pumila and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 5) 300 g of 50% ethanol aqueous solution to 10 g of whole grass (dry product) of Munronia pumila
Was added and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 6) To 10 g of Munronia pumila whole grass (dry product), 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

Production Example 7 To 10 g of Borrelia hispida root and stem (dry product) was added 300 ml of ethanol, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 8) 10 g of a 50% ethanol aqueous solution was added to 10 g of Borrelia hispida root and stem (dry product).
ml was added and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 9) To 10 g of Borrelia hispida root and stem (dry product), 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 10) 300 g of ethanol was added to 10 g of whole plant (dry product) of Phyllanthus nuriri,
It was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 11) 10 g of whole plant (dry product) of Phyllanthus nuriri, 300 m of 50% ethanol aqueous solution
1 was added and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 12) To 10 g of whole grass (dry product) of Phyllanthus nuriri, 300 ml of purified water was added and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 13) 300 ml of ethanol was added to 10 g of the whole grass (dry matter) of the morning glory, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 14) 300 g of 50% aqueous ethanol solution to 10 g of whole grass (dry matter) of Pharbitis nippon
Was added and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 15) 300 g of purified water was added to 10 g of whole grass (dry matter) of morning glory and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Manufacturing Example 16) 300 g of ethanol was added to 10 g of whole grass (dry product) of Spheransus indyx and left for 5 days with occasional stirring. After filtering this,
Evaporated and lyophilized.

(Production Example 17) 300 g of 50% aqueous ethanol solution was added to 10 g of whole plant (dry product) of Spheranthus indyx and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 18) 300 g of purified water is added to 10 g of whole plant (dry product) of Spheransus indyx and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 19) 300 ml of ethanol was added to 10 g of dried shoots of Muraya Coenigy and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 20) Muraya Koenigy stem and branch (dried product) 10 g, 50% ethanol aqueous solution 300 ml
Was added and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 21) Muraya Koenigii branch (dry product) (10 g) was added with purified water (300 ml) and heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Manufacturing Example 22) 300 g of ethanol was added to 10 g of whole grass (dried product) of Thoragia involculata and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 23) 10 g of a 50% ethanol aqueous solution was added to 10 g of whole grass (dried product) of Thoragia involucata.
0 ml was added and left for 5 days with occasional stirring. It was filtered, evaporated and lyophilized.

(Production Example 24) Purified water (300 ml) was added to 10 g of the whole plant (dry product) of Thoragia involucata,
Heat for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Production Example 25) 300 g of ethanol was added to 10 g of stem and branch (dry product) of Kamiboki, and the mixture was left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 26) 300 ml of 50% ethanol aqueous solution was added to 10 g of stems and branches (dry product) of Kamimeboki and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.

(Production Example 27) Purified water (300 ml) is added to the stems and branches (dry product) of Kamimeboki, and the mixture is heated for 3 hours. This was allowed to cool, then filtered and freeze-dried.

(Example 1) Preparation of lotion (% by weight) Olive oil 0.5 Extract from Production Example 1 0.5 Polyoxyethylene (20E.O.) Sorbitan monostearate 2.0 Polyoxyethylene (60E. O.) Hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

Example 2 Preparation of Cream A mixed composition of the following A and B was weighed and heated to 70 ° C., A was gradually added to B with stirring, and then 30 ° C. with slow stirring. Cooled down. A (% by weight) Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production example 2 Extract 1.0 B Purified water 47.9 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5.0 1.0 % Aqueous solution of sodium hyaluronate 5.0 methyl paraoxybenzoate 0.1

(Example 3) Example 3 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 3.

(Example 4) Example 4 is a cream prepared by replacing the extract of Production Example 2 of Example 2 with the extract of Production Example 4.

(Example 5) Example 5 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 5.

(Example 6) Example 6 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 6.

(Example 7) Example 7 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 7.

(Example 8) Example 8 is a cream prepared by replacing the extract of Production Example 2 of Example 2 with the extract of Production Example 8.

Example 9 Example 9 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 9.

(Example 10) Example 10 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 10.

(Example 11) Example 11 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 11.

(Example 12) Example 12 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 12.

(Example 13) Example 13 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 13.

Example 14 Example 14 is a cream prepared by replacing the extract of Production Example 2 of Example 2 with the extract of Production Example 14.

(Example 15) Example 15 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 15.

Example 16 Example 16 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 16.

(Example 17) Example 17 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 17.

(Example 18) Example 18 is a cream prepared by replacing the extract of Production Example 2 of Example 2 with the extract of Production Example 18.

Example 19 Example 19 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 19.

(Example 20) Example 20 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 20.

(Example 21) Example 21 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 21.

(Example 22) Example 22 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 22.

(Example 23) Example 23 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 23.

Example 24 Example 24 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 24.

Example 25 Example 25 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 25.

(Example 26) Example 26 is a cream prepared by changing the extract of Production Example 2 of Example 2 into the extract of Production Example 26.

(Example 27) Example 27 is a lotion prepared by changing the extract of Production Example 1 of Example 1 into the extract of Production Example 27.

[Antioxidant Test] The following test solution was prepared in a 50 ml test tube with a screw cap. Specimen 5 mg 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml Purified water 5 ml This is left to stand in a thermostat bath at 50 ° C in the dark. The following measurements were carried out before putting this in a constant temperature bath and every few days. Test solution 0.125 ml, 75% ethanol 12.125 ml,
After adding 0.125 ml of 30% ammonium thiocyanate and stirring and leaving it for 3 minutes, 0.02N ferrous chloride 3.5%
0.125 ml of an aqueous HCl solution was added, and the mixture was stirred and allowed to stand for 3 minutes, and then the absorbance was measured at a wavelength of 500 nm. Cell length 10m
m, the control cell was prepared by replacing the test solution with water. The results are shown in Tables 1 to 7. BHT is 2,6 di-tert-butyl 1-hydroxytoluene, which is a synthetic antioxidant. Riken E Oil 700 is Vitamin E (safe but less effective).

[0074]

[Table 1]

[0075]

[Table 2]

[0076]

[Table 3]

[0077]

[Table 4]

[0078]

[Table 5]

[0079]

[Table 6]

[0080]

[Table 7]

[Usage Test] 56 women were asked to become a panel, and 1 team was made up of 4 teams, 14 teams were divided into left and right faces for 4 people, and the lotion and cream of the embodiment were set on one side every day. Apply more than once, on the other hand,
The lotions of Comparative Example 1 and the cream of Comparative Example 2 in which the extracts of the production examples of Examples 1 and 2 were replaced with water were set as a set and applied once or more daily. Table 8 shows the experiment No. and the cosmetics of Examples and Comparative Examples used in each experiment. Three months later, he was asked to give a score according to the following criteria, and the total score was used for evaluation. Table 9 shows the evaluation results for each item of the effect of preventing rough skin, the luster of the skin, and the degree of skin elasticity. Example is very good 3 Example is considerably good 2 Example is slightly good 1 No difference 0 Comparative example is good −1 Comparative example is good −2 Comparative example Is very good -3

[0082]

[Table 8]

[0083]

[Table 9]

[0084]

[Effects of the Invention] All the solvent extracts of the plant of the present invention exhibit an excellent antioxidative effect, and when a cosmetic composition containing the same is applied, the effect of preventing rough skin and retaining the shine and the suppleness of the skin is excellent. ing. These plants have been used as medicines since ancient times, and their safety to the human body is guaranteed.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masao Hattori 2556-4-2 2-203 Gofuku Suehiro-cho, Toyama City, Toyama Prefecture (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Yamabe 1-8-21 Kawasaki, Ise City, Mie Prefecture (72) Inventor, Koichi Iida 56-1 Kurose Town, Ise City, Mie Prefecture

Claims (1)

[Claims] 1. An antioxidant comprising at least one solvent extract selected from the group consisting of Nigera sativa, Munronia pumila, Borrelia hispida, Filansus nuriri, Asagao calaxa, Spheransus indix, Muraya Koenigy, Tragia involucata and Kamimeboki.