JP3541049B2 - Hyaluronidase inhibitor - Google Patents

Description

【００３３】
【表２】

【００３４】
【表３】

【００３５】
【表４】

【００３６】
【表５】

【００３７】
【表６】

【００３８】
〔使用テスト〕
女性３５名にパネルとなってもらい、１班５名の７班に分け、５名づつの顔面を左右に分け、一方に実施例のローションとクリームをセットにして毎日、１回以上施用してもらい、他方には実施例１，２の製造例１，２の抽出物を水にかえたものを比較例１，２のローションとクリームにして、毎日１回以上施用してもらい、３月後、下記の判定基準により、肌のつや、肌のはりについて評点をつけてもらった。その合計値を評価値とした。実験No．は次の表７の通りの組合わせとした。判定基準は次の通りである。肌のつや、肌のはりについての官能評価値を表８に示す。
実施例の方が非常によい ３
実施例の方がかなりよい ２
実施例の方がややよい １
差がない ０
比較例の方がややよい −１
比較例の方がかなりよい −２
比較例の方が非常によい −３
【００３９】
【表７】

【００４０】
【表８】

【００４１】
【発明の効果】
本発明の植物体は古くから医薬品として人体に適用されて、安全性が確認されている。その溶媒抽出物は優れたヒアルロニダーゼ阻害作用が認められ、これを配合した化粧料と施用すると、肌のつややはりを保持する効果が大きい。[0001]
[Industrial applications]
The present invention has been used for many years as a drug for other purposes, etc., and uses a plant extract whose safety is guaranteed, using a plant extract that maintains the lubricity and flexibility of the skin and degrades hyaluronic acid that prevents aging. The present invention relates to a hyaluronidase inhibitor that suppresses the activity to prevent fine wrinkles and swelling of the skin.
[0002]
[Prior art]
Cymbopogon nardus is a monocotyledonous plant net, Poaceae, Poaceae, and Ogarkaya plants, and is used as a raw material for insect repellents, fragrances, and soaps for mosquitoes. .
[0003]
Sphaeranthus indicus is an Asteraceae plant that is commonly found in the wetlands of lowland paddy fields in India, Sri Lanka, Malaysia, Africa and China, where roots and seeds are used as anthelmintics and bark is used to treat hemorrhoids. As a medicine, whole grass is used as a poison for fish.
[0004]
Ocimum sanctum is a member of the Labiatae family and is a common plant in India and Sri Lanka. It is an important plant in Hindu. Its leaves are used for malaria, dysentery, indigestion, etc. It's being used.
[0005]
Tinospora cordifolia is a vine perennial plant belonging to the family Araceae and has been used for diuresis, laxity, malaria and the like.
[0006]
Murraya koenigii is a plant of the dicotyledonous plant net, the leaf-flowering subassembly, Pterodactyla, and Rutaceae, distributed in India and Sri Lanka, and is commonly found in dry lowlands. It has been used as a medicament for constipation, stomach colic, diarrhea, etc.
[0007]
On the other hand, hyaluronidase is an enzyme widely distributed in living organisms and also present in skin, and as its name implies, degrades hyaluronic acid. Hyaluronic acid is a linear high-molecular polysaccharide in which β-DN-acetylglucosamine and β-D-glucuronic acid are alternately bonded, and both glycosaminoglucan and chondroitin sulfate are widely present in connective tissues of mammals. It is a kind. As a function of hyaluronic acid in connective tissue, water is retained in the intercellular space, and a jelly-like matrix is formed in the tissue to retain cells. It is thought to prevent bacterial disorders) and bacterial infections. It is said that hyaluronic acid in the skin decreases with age, which results in aging such as fine wrinkles and bulkiness.
[0008]
Therefore, suppressing the activity of hyaluronidase, which degrades this, can improve the stability of hyaluronic acid used in the preparation, the stability of hyaluronic acid in the preparation after application to the skin, and the hyaluronic acid present in the skin. It is thought to contribute. In addition, hyaluronidase is also known as an inflammatory enzyme, and it is known that suppressing the activity suppresses inflammation and also works for allergy.
[0009]
[Problems to be solved by the invention]
An object of the present invention is to provide a hyaluronidase inhibitor which has been found to be natural and safe for the human body and has a strong hyaluronidase activity inhibitory action.
[0010]
[Means for Solving the Problems]
The present inventors, in order to solve the above problems, have been used for food or medicine for many years already, or applied to the human body, screening and examining animals and plants that have been confirmed to be safe for the human body, hyaluronidase Those that have utility as inhibitors were examined. As a result, it has been found that the above-mentioned plant has been used as a pharmaceutical for many years and its safety has been confirmed, and that the solvent extract is very effective as a hyaluronidase activity inhibitor.
[0011]
That is, the present invention is a hyaluronidase inhibitor comprising at least one solvent extract selected from the group consisting of Scutellaria japonica, Muraya koenigi, Sphalanthus indices, Kamamebuki, and Ibonashidurafuji.
[0012]
As a method of utilizing these plants, extraction is performed with water or a hydrophilic organic solvent such as ethanol, methanol, acetone, or the like. However, it is a matter of course that extraction with water, ethanol or a mixed solvent thereof is preferable because it is the extraction of cosmetic raw materials. In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction. It is also effective to freeze-dry and use it as a powder depending on the method of use.
[0013]
This material is added with other cosmetic ingredients such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various chemicals. Various dosage forms of cosmetics can be prepared. For example, a use form may be considered depending on the purpose, such as a lotion, a cream, a milky lotion, and a pack.
[0014]
【Example】
An embodiment which is an actual use method will be described below, but the present invention is not limited to the embodiment. Production examples of the solvent extract of each plant used in the present invention are shown below.
[0015]
[Production Example 1]
10 g of Rhizoma rhizome (dried product) was added to 300 ml of 50% ethanol and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.
[0016]
[Production Example 2]
300 ml of purified water is added to 10 g of the rhizome (dried product) of Kosuigaya and heated for 3 hours. After allowing this to cool, it was filtered and freeze-dried.
[0017]
[Production Example 3]
300 ml of purified water is added to 10 g of a stem (dry product) of Murraya koenigii, and the mixture is heated for 3 hours. After allowing this to cool, it was filtered and freeze-dried.
[0018]
[Production Example 4]
300 ml of purified water is added to 10 g of the whole plant (dried product) of Sphaeranthus indicus and heated for 3 hours. After allowing this to cool, it was filtered and freeze-dried.
[0019]
[Production Example 5]
300 ml of 50% ethanol was added to 10 g of the stem (dried product) of the seagull broom, and the mixture was allowed to stand for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.
[0020]
[Production Example 6]
300 ml of purified water is added to 10 g of the stem (dried product) of the seagull broom, and the mixture is heated for 3 hours. After allowing this to cool, it was filtered and freeze-dried.
[0021]
[Production Example 7]
300 g of 50% ethanol was added to 10 g of the stem (dried product) of Ibonashi tsumurai and left for 5 days with occasional stirring. This was filtered, evaporated and freeze-dried.
[0022]
[Production Example 8]
300 ml of purified water is added to 10 g of the dried stalk of Ibonashi tsutsuji and heated for 3 hours. After allowing this to cool, it was filtered and freeze-dried.
[0023]
[Example 1] Preparation of lotion (% by weight)
Olive oil 0.5
Extract of Production Example 2 0.5
Polyoxylene (20EO) sorbitan monostearate 2.0
Polyoxylen (60EO) hydrogenated castor oil 2.0
Ethanol 10.0
1.0% aqueous sodium hyaluronate solution 5.0
Purified water 80.0
[0024]
[Example 2] Preparation of cream (% by weight)
A squalane 20.0
Olive oil 2.0
Mink oil 1.0
Jojoba oil 5.0
Beeswax 5.0
Cetostearyl alcohol 2.0
Glycerin monostearate 1.0
Sorbitan monostearate 2.0
Extract of Production Example 1 1.0
B Purified water 47.9
Polyoxylene (20EO) sorbitan monostearate 2.0
Polyoxylene (60EO) hydrogenated castor oil 1.0
Glycerin 5.0
1.0% aqueous sodium hyaluronate solution 5.0
Methyl paraoxybenzoate 0.1
A and B were each weighed and heated to 70 ° C., A was gradually added to B with stirring, and then cooled to 30 ° C. with slow stirring.
[0025]
[Example 3]
Example 3 is a cream prepared by changing the extract of Production Example 1 of Example 2 to the extract of Production Example 3.
[0026]
[Example 4]
Example 4 is a lotion prepared by changing the extract of Production Example 2 of Example 1 to the extract of Production Example 4.
[0027]
[Example 5]
Example 5 is a cream prepared by changing the extract of Production Example 1 of Example 2 to the extract of Production Example 5.
[0028]
[Example 6]
Example 6 is a lotion prepared by changing the extract of Production Example 2 of Example 1 to the extract of Production Example 6.
[0029]
[Example 7]
Example 7 is a cream prepared by changing the extract of Production Example 1 of Example 2 to the extract of Production Example 7.
[0030]
Example 8
Example 8 is a lotion prepared by changing the extract of Production Example 2 of Example 1 to the extract of Production Example 8.
[0031]
(Hyaluronidase activity inhibition test)
(Test method) 6 g of a 0.4% sodium hyaluronate 0.1 M (pH 6.0) phosphate buffer solution was weighed and left in a thermostatic water bath at 37 ° C. for 5 minutes. 1.0 ml of a 1 wt / v% aqueous solution (if it is difficult to dissolve, add ethanol to dissolve, add purified water, evaporate, remove ethanol, and adjust to 0.1 wt / v%). 1 ml of 0.01 M hyaluronidase (Sigma, bovine testes, type IS) 0.1 M (pH 6.0) phosphate buffer solution was added, and the mixture was immediately stirred and 6 ml was placed in a 37 ° C. constant temperature water bath. The sample was placed in an Ostwald viscometer. After 5 minutes, 10 minutes, 20 minutes, and 40 minutes, the viscosity was measured. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner. In this test, the final concentration of the sample is 0.0125%. The results are shown in Tables 1 to 6 as indices, with the viscosity after 1 minute as 100.
[0032]
[Table 1]

[0033]
[Table 2]

[0034]
[Table 3]

[0035]
[Table 4]

[0036]
[Table 5]

[0037]
[Table 6]

[0038]
[Use test]
We asked 35 women to be a panel, divided into 7 groups of 1 group and 5 groups, divided the faces of 5 persons into left and right, and applied the lotion and cream of the example to one side at least once daily. On the other hand, the extracts of Production Examples 1 and 2 of Examples 1 and 2 were replaced with water to make the lotions and creams of Comparative Examples 1 and 2, and applied at least once a day. According to the following criterion, a rating was given for skin gloss and skin abrasion. The total value was used as the evaluation value. Experiment No. Are combinations as shown in Table 7 below. The criteria are as follows. Table 8 shows the sensory evaluation values of the skin gloss and the skin abrasion.
Example is much better 3
Example is considerably better 2
Example is slightly better 1
No difference 0
Comparative example is slightly better -1
Comparative example is much better -2
Comparative example is much better -3
[0039]
[Table 7]

[0040]
[Table 8]

[0041]
【The invention's effect】
The plant of the present invention has been applied to the human body as a pharmaceutical for a long time, and its safety has been confirmed. The solvent extract has an excellent hyaluronidase inhibitory action, and when applied with a cosmetic containing the same, it has a large effect of maintaining the skin's luster.

Claims (1)

A hyaluronidase inhibitor comprising at least one solvent extract selected from the group consisting of Kosuigaya, Muraya koenigi, Spharanthus Indices, Kamimebuki, and Ibonashidurafuji.