JP2003128569A - Skin care preparation, drink and food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an antioxidant which contains as an active ingredient a substance having an anti-oxidizing action and found in natural products, and to provide a skin care preparation, drink and food which contains the antioxidant. SOLUTION: The skin care preparation, drink and food each is characterized by containing an extract obtained from a plant belonging to the genus Sauropus as an active anti-oxidizing ingredient.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an antioxidant and an anti-aging agent containing an extract from a plant as an active ingredient, and a skin external preparation and a food or drink containing the extract from a plant.

[0002]

2. Description of the Related Art In recent years, especially as a factor that oxidizes biological components
As a result, active oxygen is drawing attention, and its adverse effects on the living body.
Is a problem. Active oxygen is an energy source in living cells
It occurs in the gee-metabolic process and is a superoxide.
(That is, superoxide generated by one-electron reduction of oxygen molecule
Anion (・ O ₂-), Hydrogen peroxide (H₂O₂), Singlet oxygen (
¹O₂), Hydroxy radical (.OH), etc. These activities
Oxygen is essential for the bactericidal mechanism of phagocytes and is a virus
Plays an important role in removing cancer cells and cancer cells
Excessive elemental production is the amount of biogenic components that make up the membranes and tissues in the body
Attacks offspring and induces various diseases. For example, active oxygen
Decomposes, denatures or cross-links biological tissues such as collagen
Or peroxidized fats that oxidize fats and oils and damage cells
It is thought to generate quality, and active oxygen causes
These disorders caused by skin wrinkling and skin
It is thought to cause aging such as reduced elasticity of the skin
There is.

As a cause of aging such as wrinkling of the skin and reduction of elasticity of the skin, various causes other than active oxygen can be considered. That is, the dermis and epidermis of the skin are composed of epidermal cells, fibroblasts, and an extracellular matrix that is outside these cells and supports the skin structure such as elastin, collagen, and mucopolysaccharides such as hyaluronan and other mucopolysaccharides. In young skin, the interaction between these skin tissues maintains homeostasis, thereby retaining water,
Flexibility, elasticity, etc. are secured, and the appearance of the skin is firm and shiny, and is kept fresh.

However, when there is an influence of some external factors such as ultraviolet rays, drastic drying of air, excessive skin washing, etc., and aging progresses, the production amount of collagen, which is a main component of the extracellular matrix, is reduced. As well as decreasing, the elasticity decreases due to crosslinking. Moreover, mucopolysaccharides such as hyaluronic acid have many functions such as retention of water in the intercellular spaces and retention of lubricity and flexibility of tissues, but these decrease with age. As a result, the skin loses its moisturizing function and elasticity, and the keratin begins to exfoliate abnormally, causing the skin to lose its tension and luster, and to exhibit aging symptoms such as roughness, wrinkles, and dullness.

As described above, the changes caused by aging of the skin, that is, wrinkles, dullness, loss of texture, decrease in elasticity, etc.
It is involved in the reduction and denaturation of dermal matrix components such as collagen and hyaluronic acid and other mucopolysaccharides. Therefore, promotion of collagen and hyaluronic acid production is important for preventing and improving skin aging.

[0006] Further, one of the causes of skin aging with aging is that the secretion of estrogen, which is a female hormone, is decreased. That is, estrogen is deeply involved in maintaining the health of adult women, and its insufficient secretion leads to various medical disorders, skin hypersensitivity, decreased elasticity, decreased moisture, etc.
It is known to cause unwanted skin changes.

[0007]

[Problems to be Solved by the Invention] Preventing and treating skin aging such as wrinkle formation and elasticity reduction by suppressing the production of active oxygen and radicals in the body and suppressing the production of lipid peroxide. It is considered possible. Also, by controlling the extracellular matrix by promoting the production of collagen and hyaluronic acid, or by supplementing the decrease in estrogen secretion due to aging, it is possible to prevent and treat skin aging such as wrinkle formation and elasticity reduction. Conceivable.

[0008] Therefore, the first object of the present invention is to find out a natural substance having an active oxygen scavenging action or a radical scavenging action, and to provide an antioxidant containing it as an active ingredient.

Secondly, the present invention finds a natural product having a collagen production promoting action, a hyaluronic acid production promoting action or an estrogen-like action, and provides an anti-aging agent containing it as an active ingredient. The purpose is to

A third object of the present invention is to find a natural product having an antioxidant action or an anti-aging action, and to provide a skin external preparation containing it.

A fourth object of the present invention is to find a natural product having an antioxidant action or an anti-aging action, and to provide a food or drink containing the same.

[0012]

In order to solve the above-mentioned problems, the antioxidant and anti-aging agent of the present invention are characterized by containing an extract from a plant belonging to the genus Sauropus as an active ingredient. Topical skin products and foods and drinks from Saur
It is characterized by containing an extract from a plant belonging to the genus opus.

In the antioxidant of the present invention, the extract preferably has an active oxygen scavenging action and / or a radical scavenging action. The anti-aging agent of the present invention is selected from the group consisting of collagen production promoting action, hyaluronic acid production promoting action and estrogen-like action.
It is preferable to have one or two or more actions.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. In the present invention, "an extract from a plant belonging to the genus Sauropus" means an extract obtained by using a plant belonging to the genus Sauropus as an extraction raw material, a diluted solution or a concentrated solution of the extract, and an extract obtained by drying the extract. The dried product, or the crude product or the purified product thereof is included.

The plant used as an extraction raw material is not particularly limited as long as it belongs to the genus Sauropus.
Us rostrata Miq.), Sauropus and rogynus, etc. can be used as extraction raw materials, but
trata Miq.) is preferably used as the extraction raw material. Ryuri leaf is an evergreen small shrub belonging to the Euphorbiaceae family,
It is distributed from South China, Southeast Asia to Malaysia, and can be obtained from these areas. In China, this leaf is used as a medicinal leaf for colds, constipation, etc., but it has not been known until now that it has antioxidant and anti-aging effects.

The constituent parts of the plant used as the extraction raw material are not particularly limited, and for example, constituent parts such as leaves, stems, flowers, bark, seeds, fruits, and fruit kernels can be used as the extraction raw material. it can. Of these, it is particularly preferable to use the leaf portion as the extraction raw material.

The details of the substance having an antioxidant action and / or an anti-aging action contained in the extract from the plant belonging to the genus Sauropus are not known, but by the extraction method generally used for plant extraction, Sauropus An extract having an antioxidant effect and / or an anti-aging effect can be obtained from a plant belonging to the genus. For example, after drying the extraction material,
It can be obtained as it is or by crushing it using a coarse crusher and subjecting it to extraction with an extraction solvent. On this occasion,
The extraction raw material may be dried in the sun or using a commonly used dryer. A plant belonging to the genus Sauropus may be used as an extraction raw material after being subjected to a pretreatment such as defatting with a nonpolar solvent such as hexane or benzene. By performing a pretreatment such as defatting, it is possible to efficiently perform the extraction treatment of a plant belonging to the genus Sauropus with a polar solvent.

As the extraction solvent, it is preferable to use water, a hydrophilic organic solvent or a mixture thereof at room temperature or at a temperature not higher than the boiling point of the solvent.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, as well as those that have been subjected to various treatments.
Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering and the like.
Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent which can be used as the extraction solvent include, for example, methanol, ethanol, propyl alcohol, isopropyl alcohol and the like having 1 to 5 carbon atoms.
And lower aliphatic ketones such as acetone and methyl ethyl ketone; and polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol and glycerin.

When a mixed solution of water and a hydrophilic organic solvent is used as an extraction solvent, water is used in the case of a lower alcohol.
1 to 90 parts by weight with respect to 0 parts by weight, 1 to 40 parts by weight with respect to 10 parts by weight of water in the case of a lower aliphatic ketone, and 10 to 90 parts by weight with respect to 10 parts by weight of water with a polyhydric alcohol. It is preferable to add.

In the present invention, it is not necessary to employ a special extraction method for extracting a substance having an antioxidant action and / or an anti-aging action, and it is possible to perform extraction using an arbitrary device at room temperature or under reflux heating. You can

Specifically, the extraction raw material is put into a treatment tank filled with an extraction solvent, and if necessary, the mixture is stirred with occasional stirring.
After the soluble component is eluted by standing for 0 to 2 hours, the solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times the amount of the extraction raw material (weight ratio),
When water is used as an extraction solvent, the extraction conditions are usually 50 to 95 ° C. and about 1 to 4 hours. When a mixed solvent of water and ethanol is used as the extraction solvent,
It is usually at 40 to 80 ° C. for about 30 minutes to 4 hours.

The obtained extract is diluted, concentrated, dried according to a conventional method in order to obtain a diluted solution or concentrated solution of the extracted solution, a dried product of the extracted solution, or a crudely purified product or a purified product thereof. You may perform processing, such as refinement | purification.

The obtained extract can be used as it is as an antioxidant or an anti-aging agent, but a concentrated solution or a dried product is easier to use. Formulation of an extract from a plant belonging to the genus Sauropus can be performed according to a conventional method. In the case of formulation, in order to facilitate storage and handling, dextrin, a pharmaceutically acceptable carrier such as cyclodextrin and any other auxiliary agent can be added, and an extract from a plant belonging to the genus Sauropus can be added. It can be formulated into any dosage form such as powder, granules and tablets.

[0026] Since an extract from a plant belonging to the genus Sauropus has a unique odor, it is possible to carry out purification for the purpose of decolorization, deodorization, etc. within a range that does not reduce the physiological activity thereof. When it is added to the external preparation for skin, food and drink, etc., it is not used in a large amount, so there is no practical problem even if it remains unpurified. Specifically, the purification is activated carbon treatment,
It can be performed by adsorption resin treatment, ion exchange resin treatment, or the like.

The extract obtained from the plants belonging to the genus Sauropus as described above has an antioxidant effect or an anti-aging effect, and is used as an antioxidant or an anti-aging agent by utilizing the respective effects. can do.

Here, the antioxidant effect of the extract from the plant belonging to the genus Sauropus is exerted based on, for example, the active oxygen scavenging activity and / or the radical scavenging activity. However, the antioxidant action of the extract from the plant belonging to the genus Sauropus is not limited to the antioxidant action exerted based on the above action. Here, "active oxygen" includes superoxide, hydrogen peroxide, hydroxy radicals, singlet oxygen, and the like. Further, the “radical” means a molecule or atom having one or more unpaired electrons, such as superoxide, hydroxy radical, DPP.
H, etc. are included. Since the extract from the plant belonging to the genus Sauropus has an active oxygen scavenging action or a radical scavenging action, it can be used as an active oxygen scavenger or an active ingredient of a radical scavenger. An agent or a radical scavenger may be an active ingredient of the antioxidant of the present invention.

The anti-aging action of the extract from the plant belonging to the genus Sauropus is, for example, collagen production promoting action,
It is exerted based on one or more actions selected from the group consisting of a hyaluronic acid production promoting action and an estrogen-like action. That is, an extract from a plant belonging to the genus Sauropus has an action of promoting the production of collagen and / or hyaluronic acid by fibroblasts so that the dermal layer is supplemented with sufficient collagen and / or hyaluronic acid. At the same time, it has the effect of preventing and improving skin aging due to a decrease in estrogen secretion. However, Sa
The anti-aging action of the extract from the plant belonging to the uropus genus is not limited to the anti-aging action exerted based on the above action. Since the extract from the plant belonging to the genus Sauropus has a collagen production promoting action, a hyaluronic acid production promoting action or an estrogen-like action, a collagen production promoting agent, a hyaluronic acid production promoting agent or an estrogenic agent They can also be used as active ingredients, and these collagen production promoters, hyaluronic acid production promoters or estrogen-like agents may be used as the active ingredients of the anti-aging agent of the present invention.

Extracts from plants belonging to the genus Sauropus are
Since it can prevent and / or improve skin aging, and has excellent usability and safety when applied to the skin, it is suitable for incorporation in an external preparation for skin. The external preparation for skin may contain one kind of the antioxidant or the anti-aging agent of the present invention, or a combination of two or more kinds.

Here, the "skin external preparation" means various drugs applied to the skin, and includes, for example, cosmetics, quasi drugs, pharmaceuticals and the like. Specific examples of the external preparation for skin include those for the skin, for example, ointments, paps, creams, emulsions, lotions, packs, jellies, and the like for the scalp, for example, tonics, rinses, shampoos, astringents and the like. Can be mentioned.

The amount of the extract from the plant belonging to the genus Sauropus in the external preparation for skin of the present invention can be appropriately adjusted depending on the type of external preparation for skin, physiological activity of the extract and the like. Converted to a standard extract, about 0.
It is 005 to 10% by weight.

The external preparation for skin of the present invention comprises various main agents and auxiliary agents usually used in the preparation of the external preparation for skin, as long as they do not interfere with the antioxidative action and antiaging action of the extract from the plant belonging to the genus Sauropus. Agents and other optional auxiliaries can be used. The external preparation for skin of the present invention is not limited to the one in which an extract from a plant belonging to the genus Sauropus is the main agent for preventing and improving skin aging.

In the external preparation for skin of the present invention, the following can be exemplified as those which can be used as a constituent component for an external preparation for skin together with an extract from a plant belonging to the genus Sauropus. When the following constituents are used in combination with an extract from a plant belonging to the genus Sauropus, a synergistic action between the constituents used in combination with an extract from a plant belonging to the genus Sauropus is usually higher than expected. It may bring about an excellent use effect.

Astringent: citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof, aluminum chloride, aluminum / potassium sulfate, allantoinchlorohydroxyaluminum, allantoindihydroxyaluminum, zinc paraphenolsulfonate, zinc sulfate, zinc extract, Ages extract, hamamelis extract, genoderma Root extract, tea catechins, sedum extract, St. John's wort extract, rhubarb extract, cornflower extract, kizuta extract, cucumber extract, horse chestnut extract, salvia extract, melissa extract and the like.

Bactericidal / antibacterial agents: benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, chlorhexidine hydrochloride, photosensitive element 101, photosensitive element 201, salicylic acid, sodium salicylate, sorbic acid, Halocarban, resorcin, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, chitosan degradation product, jiuyu extract, kujin extract, nematode extract, loquat extract, yucca extract, aloe extract, cinnamon essence extract, zedoary extract and the like.

Whitening agent: ascorbic acid or its derivative,
Sulfur, placenta hydrolyzate, ellagic acid or its derivative, kojic acid or its derivative, glucosamine or its derivative,
Arbutin or a derivative thereof, hydroxycinnamic acid or a derivative thereof, glutathione, arnica extract, sardine extract, sow syrup extract, psycho extract, bow-fu extract, Ganoderma lucidum mycelium culture or an extract thereof, linden extract, peach leaf extract, age extract, kujin extract. , Jew extract, Toki extract, Yokinin extract, Oyster leaf extract, Rheum palm extract, Buttonpi extract, Hamamelis extract, Horse chestnut extract, Hypericum perforatum extract, Oil-soluble licorice extract (liquorice hydrophobic flavone, glabridin, glabrene, Ricochalcone A) and the like.

UV absorber: β-isopropylfuranone derivative, urocanic acid, ethyl urocanate, oxybenzone, oxybenzone sulfonic acid, tetrahydroxybenzophenone, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, cinoxate, methyl diisopropylcinnamate, methoxycinnamic acid Methyl, octyl methoxycinnamate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, titanium oxide, β-carotene, γ-oryzanol, rice bran extract, aloe extract, Birch extract, birch extract, chamomile extract, henna extract, turmeric extract, ginkgo biloba extract , Hawthorn extract, oil-soluble licorice extract, and the like.

Moisturizers: serine, glycine, threonine,
Alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipids, glyceroglycolipids, sphingophospholipids, sphingoglycolipids, linoleic acid or its esters, eicosapentaenoic acid or its Esters, pectin, bifidobacteria fermented product, lactic acid fermented product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol,
Birodoaoi extract, Yokuinin extract, scented extract, turmeric extract, Kaiso extract, Kidachialoe extract, Burdock extract, Mannen wax extract, Arnica extract, wheat bran, rice bran extract and the like.

Cell activating agent: riboflavin or its derivative, pyridoxine or its derivative, nicotinic acid or its derivative, pantothenic acid or its derivative, α-tocopherol or its derivative, arnica extract, carrot extract, rapeseed ginseng extract, loofah extract (saponin). ), Shikon extract, psyllium extract, peony extract, peony extract, mukuroji extract, safflower extract, ashitaba extract, loquat leaf extract, Hikiokoshi extract, Yukinoshita extract, yellow musk extract, salvia extract,
Garlic extract, mannen wax extract, etc.

Anti-inflammatory / anti-allergic agents: azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzene, glycyrrhizic acid or its derivative, glycyrrhetinic acid or its derivative, Photosensitivity No. 301, Photosensitivity No. 401,
Diphenhydramine hydrochloride, tranexamic acid or its derivatives, adenosine phosphate, estradiol, estrone,
Ethinyl estradiol, cortisone, hydrocortisone, prednisolone, progesterone, corticosterone, arnica extract, inchinko extract, sushi extract, sycamore extract, licorice extract, ginseng extract, mugwort extract, oleander extract, gentian extract, psychological extract, senkyu extract, seikou extract. Yarrow extract, Coptis extract, Perilla extract, etc.

Antioxidant / active oxygen scavenger: dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, baicalin, baicalein, superoxide dismutase, catalase, rosemary extract, melissa extract, sardine extract, age extract, loquat leaf. Extract, hop extract, hamamelis extract,
Peony extract, sage extract, kina extract, chamomile extract, eucalyptus extract, perilla extract, ginkgo biloba extract, thyme extract, cardamom extract, caraway extract, nutmeg extract, mace extract, laurel extract, clove extract, turmeric extract, willow extract.

In the case of producing a cosmetic containing an extract from a plant belonging to the genus Sauropus, there is almost no restriction on the selection of other raw materials for producing cosmetics, and general cosmetic bases exemplified below. Any of these can be used.

Oils and fats: soybean oil, linseed oil, tung oil, sesame oil, bran oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil,
Peanut oil, cacao oil, owl, palm oil, palm kernel oil,
Beef tallow, mink oil, egg yolk oil, jojoba oil, evening primrose oil, horse oil.

Waxes: carnauba wax, candelilla wax, beeswax, honey beeswax, whale wax, cerax, lanolins.

Hydrocarbons: liquid paraffin, petrolatum,
Microcrystalline wax, ceresin, squalene, polystyrene powder.

Fatty acids: stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, heberic acid,
Lanolinic acid, oleic acid, undecylenic acid, isortealic acid.

Alcohols: lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol, oleyl alcohol,
Hexadecyl alcohol, 2-octyldodecanol,
Glycerin, sorbitol, propylene glycol,
1,3-butylene glycol, ethylene glycol and polymers thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol.

Esters: decyl oleate, butyl stearate, isopropyl myristate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, propylene glycol dioleate, ethylene glycol monostearate, glyceryl monostearate, glyceryl tristearate, Lanolin acetate, cetyl lactate.

Surfactants: anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants.

Fragrances: menthol, carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil.

Extracts from plants belonging to the genus Sauropus are:
Since it has been confirmed that it is not digested in the digestive tract, it is suitable for incorporation into any food or drink or dietary supplement. In this case, it is appropriate that the amount of the extract ingested per day for an adult is about 1 to 1000 mg in consideration of the general intake of the food or drink to be added.

There are no particular restrictions on foods and drinks to which an extract from a plant belonging to the genus Sauropus can be mixed, but specific examples thereof include soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks and the like beverages (these beverages). Containing concentrated concentrate of beverage and powder for adjustment); frozen desserts such as ice cream, ice sorbet, shaved ice; buckwheat, udon, harusame, gyoza skin,
Noodles such as candy skin, Chinese noodles and instant noodles; candy, chewing gum, candy, gum, chocolate, tablet confectionery,
Confectioneries such as snacks, biscuits, jellies, jams, creams, baked goods; processed fish and livestock products such as kamaboko, ham, sausages; dairy products such as processed milk and fermented milk;
Oils and fats and processed foods such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing; seasonings such as sauces and sauces; soups,
Examples include stews, salads, side dishes, and pickles.

The antioxidants, anti-aging agents, external preparations for skin and foods and drinks of the present invention described above are preferably applied to humans. It can also be applied to animals other than.

[0055]

EXAMPLES The present invention will be specifically described below with reference to production examples, test examples and formulation examples, but the present invention is not limited to the following examples.

[Production Example 1] Dragon leaf (Sauropus rostrata
Miq.) Leaf dried material finely chopped 200 g of water, 5
0% ethanol (weight ratio of water to ethanol 1: 1),
Add 2000 mL of ethanol, and use a reflux extractor at 80 ° C.
It was extracted by heating for 2 hours and filtered while hot. The same extraction treatment was further performed on the residue. The obtained extracts were combined, concentrated under reduced pressure, and further dried to obtain an extract at each site.
The yield of the extract was as shown in Table 1.

[Table 1] Sample NO. Extraction Solvent extraction rate (%) 1 Water 30.5 2 50% Ethanol 31.8 3 Ethanol 29.2

TEST EXAMPLE 1 Superoxide Elimination Test (NBT Method) The extract obtained in Production Example 1 was tested for its superoxide erasing action by the following test method.

3 mM xanthine, 3 mM EDTA,
1.5 mg / mL BSA solution and 0.75 mM nitroblue tetrazolium (NBT) each 0.1 mL, and.
2.4 mL of 05 M Na ₂ CO ₃ buffer solution (pH 10.2) was placed in a test tube, and 0.1 mL of the sample solution was added thereto,
It was left at 25 ° C for 10 minutes. Next, add 0.1 mL of xanthine oxidase solution, stir quickly, and add 2 mL at 25 ° C.
Let stand for 0 minutes. Then, 0.1 mL of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. Hereinafter, this absorbance is referred to as "absorbance upon addition of sample solution and enzyme solution".

The same operation and the measurement of the absorbance were carried out without adding the enzyme solution. The absorbance measured at this time is referred to as "absorbance when sample solution is added and enzyme solution is not added".

The same measurement was carried out when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as "absorbance when sample solution is not added and enzyme solution is added".

Further, the same measurement was carried out when the distilled water was added without adding the enzyme solution and further without adding the sample solution. The absorbance measured at this time is referred to as "absorbance when no sample solution is added and no enzyme solution is added". Then, the superoxide scavenging rate was obtained by the following formula.

Erasure rate (%) = {1- (AB) / (C-
D)} × 100

In the above formula, "A" is "absorbance when sample solution is added and enzyme solution is added", "B" is "absorbance when sample solution is added and enzyme solution is not added", and "C" is "sample solution is not added". Addition,
"Absorptance when enzyme solution is added" and "D" represent "absorbance when sample solution is not added and enzyme solution is not added".

The erasure rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the superoxide erasure rate was 50% was determined by the interpolation method. The results are shown in Table 2.

[Table 2] Sample NO. Extract 50% Elimination Sample concentration (ppm) 1 Water 50.2 2 50% Ethanol 58.7 3 Ethanol 63.5

From the results shown in Table 2, it was confirmed that the extract from the leaves of the dragon leaf had a superoxide scavenging action. It was also confirmed that the degree of this superoxide scavenging action can be adjusted by the concentration of the extract.

[Test Example 2] Radical scavenging test for DPPH The extract obtained in Production Example 1 was tested for radical scavenging action using DPPH, which is a very stable radical, by the following test method.

3 mL of the sample solution was added to 3 mL of the 1.5 × 10 ⁻⁴ M DPPH ethanol solution, the container was immediately sealed and shaken, and the mixture was left standing for 30 minutes. After that, wavelength 520n
The absorbance at m was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, 3 mL of the sample solution was added to ethanol, and immediately thereafter, the absorbance at a wavelength of 520 nm was measured. The radical scavenging rate (%) was calculated by the following formula from each measured absorbance.

[0070] Erasure rate (%) = {1- (BC) / A} × 100

In the above formula, "A" is "absorbance of control", "B" is "absorbance of sample solution added",
"C" represents "blank absorbance".

The scavenging rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; μg / mL) at which the scavenging rate of DPPH radical was 50% was determined by the interpolation method. The results are shown in Table 3.

[Table 3] Sample No. Extract 50% Elimination Sample concentration (ppm) 1 Water 108 2 50% Ethanol 102 3 Ethanol 90.4

From the results shown in Table 3, it was confirmed that the extract from the leaves of the dragon leaf has a radical scavenging action. It was also confirmed that the degree of this radical scavenging action can be adjusted by the concentration of the extract.

[Test Example 3] Test of collagen production promoting action The plant extract of Production Example 1 was tested for collagen production promoting action by the following test method.

Human fibroblasts were treated with 10% FBS, 1% N
EAA, cultured in MEM medium containing 1 mmol / L sodium pyruvate at 37 ° C. under 5% CO ₂ -95% air, and cells were collected by trypsinization to obtain 2 × 10 ⁵ c.
Adjust to 10 wells in a 96-well microplate.
0 μL of each was seeded. 37 ° C, 5% CO ₂ -95% ai
After overnight culture under r, the medium was added to the sample-added medium (sample concentration:
100ppm) Exchanged to 150μL, 37 ℃, 5% CO
And cultured for 3 days under the ₂ -95% air.

90 μL of this culture supernatant was used for ELISA
The collagen was transferred to a plate and quantified by an ELISA method using an anti-human collagen type 1 antibody, using a calibration curve using human collagen type 1 as a standard.
The collagen production promotion rate is 100% when the sample is not added.
Was calculated as The results are shown in Table 4.

[Table 4] Sample No. Extract extract Collagen production promotion rate (%) 1 Water 302 2 50% Ethanol 337 3 Ethanol 387

From the results shown in Table 4, it was confirmed that the extract from the leaves of the dragon leaf has a collagen production promoting action.

[Test Example 4] Test of hyaluronic acid production promoting action The plant extract of Production Example 1 was tested for hyaluronic acid production promoting action by the following test method.

Normal human neonatal fibroblast (NB1RGB) 1 ×
10 ^{6 of} 10% FBS in a 75 cm ² flask
In α-MEM medium (pH 7.2) containing 37 ° C, 5% C
O ₂ at under -95% air was cultured for 7 days. Cells were collected by trypsin treatment and α-MEM containing 1% FBS was used.
The medium was adjusted to 2.2 × 10 ⁴ cells / mL, 100 μL of each was seeded on a 96-well microplate, and the mixture was incubated at 37 ° C. for 5
And cultured overnight under% _CO 2 -95% air. On the next day, 100 μL of α-MEM medium containing 1% FBS in which a sample (sample concentration: 400 ppm) was dissolved was added to each well and cultured at 37 ° C. under 5% CO ₂ -95% air for 3 days.

The amount of hyaluronic acid produced was determined by ELIS
The following method utilizing Method A was performed. That is, 10 μL of this culture supernatant was diluted 10-fold with 90 μL of PBS (−), 50 μL thereof was added to an ELISA plate precoated with hyaluronic acid, and ELISA was performed using various antibodies. Hyaluronic acid was quantified using a calibration curve. The hyaluronic acid production promoting rate was calculated with the value when no sample was added as 100%. The results are shown in Table 5.

[Table 5] Sample NO. Extract Hyaluronic acid production promotion rate (%) 1 Water 118 2 50% Ethanol 143 3 Ethanol 149

From the results shown in Table 5, it was confirmed that the extract from the leaves of the dragon leaf has a hyaluronic acid production promoting action.

Test Example 5 Estrogen-Like Test The plant extract according to Production Example 1 was tested for estrogen-like action by the following test method.

The method of Thomas et al. (In Vitro C) for examining the effect on the proliferation of estrogen-dependent cells
ell. Dev. Biol. 28A, 595-602,
1992).

Human breast cancer-derived MCF-7 cells were treated with 75c
The MCF-7 cells were cultured in a m ² flask until they became confluent and treated with trypsin to collect 10
% FBS (treated with activated carbon), 1% NEAA and 1 m
It was adjusted to 3 × 10 ⁴ cells / mL using a MEM medium containing M sodium pyruvate and not containing phenol red (hereinafter abbreviated as “MEM medium”).

The prepared MCF-7 cells were seeded in a 24-well plate in an amount of 0.9 mL, and 37
Culturing was performed at 5 ° C. and 5% CO ₂ -95% air. After 6 hours (day 0), 10 times the final concentration (5
100 μL of the sample solution adjusted to 00 ppm) was added to the plate, and the culture was continued. On the 6th day from the start of the culture, the medium was replaced with a MEM medium containing 0.97 mmol / L MTT, and after culturing for 2 hours, the medium was replaced with isopropanol to extract blue formazan produced in the cells. Regarding the isopropanol containing the eluted blue formazan, the absorption maximum of blue formazan is 570n.
The absorbance at m was measured.

In order to correct the influence of adherent cells, the absorbance at 650 nm was also measured at the same time, and the difference between the two absorbances was taken as a value proportional to the amount of blue formazan produced (the absorbance in the following formula is the corrected absorbance. Is).

As a positive control, 0.02 ppm ethinyl estradiol was used. The strength of the estrogen-like action (estrogen-dependent proliferative action) was calculated by the following equation with the absorbance at 100% when no sample was added. The test results are shown in Table 6.

[0091] Estrogen-like action (%) = A / B x 100

In the above formula, "A" represents "absorbance when sample is added" and "B" represents "absorbance when sample is not added".

[Table 6] Sample No. Extract Estrogen-like action rate (%) 1 Water 111 2 50% Ethanol 122 3 Ethanol 132

From the results shown in Table 6, it was confirmed that the extract from the leaves of dragon leaf had an estrogen-like action.

Test Example 6 An emulsion containing the 50% ethanol extract obtained in Production Example 1 (hereinafter referred to as “Example emulsion”) was prepared according to a conventional method. The composition of the example emulsion is shown below. 50% ethanol extract of Ryuri leaf (Production Example 1) 1.0 g Cetyl alcohol 0.5 g Beeswax 2.0 g Polyoxyethylene sorbitan oleate (10E.0) 1.0 g Glyceryl monostearate 1.0 g Hyaluronic acid 0.1 g Propylene glycol 5.0 g Ethanol 3.0 g Methyl paraoxybenzoate 0.3 g Perfume 0.03 g Purified water balance (total amount is 100 g)

Regarding the example emulsion and the comparative example emulsion having the same composition as the example emulsion except that the leaf extract of dragon leaf was not included,
The following evaluation tests were conducted.

Subjects: Among the large number of women aged 22 to 43 years old, the sulcus and crust disappeared, and a wide range of keratin was turned over (the score shown in Table 7 was 1), or the sulcus and crest was unclear. The keratin is partially turned up (the score shown in Table 7 is 2),
Twenty people judged to have rough skin were selected as test subjects.

Application test: For each subject, the example emulsion was applied to the right half of the face, and the comparative example emulsion was applied to the left half of the face, once each in the morning and evening, and 3 times.
It was applied for 0 days.

[Judgment 1: Skin Roughness Improving Effect] After the application test, a replica of the face was taken using the replica method using Silflo (manufactured by FLEXICL DEVELOPMENTS LTD), and the state of skin marks and exfoliation of the keratin were observed with a 50 × microscope. Then, the skin condition was judged according to the evaluation criteria shown in Table 7. The determination results are shown in Table 8.

[0100] [Table 7] disappears commentary points Evaluation 1 Kawamizo-skin hill, a wide range of horny is turned over. (Rough skin condition) 2 Unsmooth skin creases / hills. The keratin is partially turned up. (Rough skin condition) 3 It is flat, although some sulcus and crust are recognized. (Ordinary skin) 4 Clear skin ridges and ridges. (Comparatively beautiful skin) 5 Skin ridges and ridges are extremely clear and well-organized. (Beautiful skin)

[Table 8] Evaluation test Before the start Example Emulsion application part Comparative example Emulsion application part 1 10 people 0 people 9 people 2 10 people 0 people 7 people 3 0 people 8 people 2 people 4 0 people 10 people 2 people 50 people 2 people 0 people

As shown in Table 8, in the area coated with the emulsion of Example, the skin roughness (aging of the skin) was remarkably improved as compared with the area coated with the emulsion of Comparative Example.

[Judgment 2 / Sensory Evaluation] Regarding the feeling of use and the effect on the skin, all the subjects were asked about the superiority or inferiority of the emulsion of the example and the emulsion of the comparative example. Table 9 shows the result of collecting the answers.

[Table 9] Evaluation item Example Emulsion is good Comparative example Emulsion is good Superiority No inferiority Skin familiarity 13 3 4 Moist feeling 18 1 1 1 Skin extension 14 2 4 Satisfaction to improve rough skin 17 2 1 Satisfaction to improve skin color 15 2 3 Improving wrinkle number and depth 19 1 0

From the results shown in Table 9, the same effects as those of the above judgment 1 and excellent feeling of use were confirmed by sensory evaluation.

From the results of Judgments 1 and 2, the external preparation for the skin containing the leaf extract of dragon leaf has the effect of preventing and improving the skin aging (effect of improving the rough skin), and has a feeling of use when applied to the skin. It was confirmed that the safety was excellent.

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method. 50% ethanol extract of Ruri leaf (Production Example 1) 1 g Jojoba oil 4 g Olive oil 2 g Squalane 2 g Cetanol 2 g Glyceryl monostearate 2 g Polyoxyethylene cetyl ether (20E.0) 2.5 g Polyoxyethylene sorbitan oleate (20E.0) 2g Yellow mud extract 0.1g Dipotassium glycyrrhizinate 0.1g Ginkgo biloba extract 0.1g Conchiolin 0.1g Oat extract 0.1g Chamomile extract 0.1g 1,3-Butylene glycol 3g Methyl paraoxybenzoate 0 .15 g Perfume 0.05 g Purified water balance (total amount is 100 g)

[Formulation Example 2] A lotion having the following composition was produced by a conventional method. Leaf water extract of Ryuri leaf (Production Example 1) 2 g Glycerin 3 g 1,3-Butylene glycol 3 g Polyoxyethylene sorbitan oleate (20E.0) 0.5 g Methyl paraoxybenzoate 0.15 g Citric acid 0.1 g Citric acid Acid soda 0.1 g Oil-soluble licorice extract 0.1 g Seaweed extract 0.1 g Xylobiose mixture 0.5 g Kujin extract 0.1 g Perfume 0.05 g Purified water balance (total amount is 100 g)

[Formulation Example 3] A cream having the following composition was produced by a conventional method. Dragon leaf leaf ethanol extract (Production Example 1) 1 g Liquid paraffin 5 g Sarah beeswax 4 g Cetanol 3 g Squalane 10 g Lanolin 2 g Stearic acid 1 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3 g 1, 3-Butylene glycol 6 g Yeast extract 0.1 g Perilla extract 0.1 g Cinnabar extract 0.1 g Chinese cabbage extract 0.1 g Methyl paraoxybenzoate 1.5 g Perfume 0.1 g Purified water balance (total amount is 100 g)

[Formulation Example 4] A pack having the following composition was produced by a conventional method. Ryuri leaf 50% ethanol extract (Production Example 1) 5 g Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Sage extract 0.1 g Touki extract 0.1 g Carrot extract 0.1 g Paraoxyethyl benzoate 0 0.05 g Perfume 0.05 g Purified water Balance (total amount is 100 g)

[Formulation Example 5] The following mixture was tabletted to produce a tablet nutritional supplement. Dragon leaf leaf ethanol extract (Production Example 1) 50 parts by weight Powdered sugar (sucrose) 178 parts by weight Solbit 10 parts by weight Glycerin fatty acid ester 12 parts by weight

[Formulation Example 6] The following mixture was formed into granules to prepare a dietary supplement. Leaf water extract of Ryuri leaf (Production Example 1) 34 parts by weight Beet oligosaccharide 1000 parts by weight Vitamin C 167 parts by weight Stevia extract 10 parts by weight

[0113]

According to the present invention, an antioxidant and an anti-aging agent are provided. Further, according to the present invention, there are provided a skin external preparation and a food or drink having an antioxidant effect and / or an anti-aging effect. According to the antioxidant of the present invention, it is possible to effectively prevent and treat aging phenomena such as skin wrinkle formation and elasticity reduction through the prevention of oxidation of biological components due to active oxygen scavenging action and in vivo radical scavenging action. It is considered to be a thing. Further, according to the anti-aging agent of the present invention, it is possible to effectively prevent and treat aging phenomena such as wrinkle formation and elasticity reduction of skin through collagen production promoting action, hyaluronic acid production promoting action or estrogen-like action. You can The antioxidant and anti-aging agent of the present invention have excellent usability and safety when applied to the skin, and therefore are suitable for incorporation in a skin external preparation. The external preparation for skin and food and drink of the present invention are useful for preventing and / or improving skin aging.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ identification code FI theme code (reference) A61K 7/00 A61K 7/48 7/48 A61P 17/16 A61P 17/16 A23L 2/00 F (72) Inventor Zhou Yang 14703-10 Muto-cho, Onomichi City, Hiroshima Prefecture Maruzen Pharmaceutical Co., Ltd. F-term (reference) 4B017 LC03 LG15 LP01 4B018 MD61 ME06 ME10 MF01 4B021 MC03 MC09 4C083 AA111 AA112 AA122 AC022 AC072 AC122 AC182 AC302 AC442 AC482 AD532 CC02 CC05 CC06 EE12 FF01 4C088 AB11 AB46 AC01 BA08 MA02 MA07 MA28 MA35 MA52 MA63 NA14 ZA89

Claims (6)

[Claims] 1. An antioxidant containing an extract from a plant belonging to the genus Sauropus as an active ingredient. 2. The extract has an active oxygen scavenging action and / or
Alternatively, the antioxidant according to claim 1, which has a radical scavenging action. 3. An anti-aging agent containing an extract from a plant belonging to the genus Sauropus as an active ingredient. 4. The extract has an action of promoting collagen production,
The anti-aging agent according to claim 3, which has one or two or more kinds of actions selected from the group consisting of a hyaluronic acid production promoting action and an estrogen-like action. 5. An external preparation for skin, comprising an extract from a plant belonging to the genus Sauropus. 6. A food or drink comprising an extract from a plant belonging to the genus Sauropus.