JPH04247010A - Beautifying and whitening cosmetics - Google Patents

Abstract

PURPOSE:To obtain a beautifying and whitening cosmetic simultaneously having inhibitory effects on melanization and rapid lightening of color of dark skin without any skin irritation. CONSTITUTION:A beautifying and whitening cosmetic is characterized by the composition obtained by blending the extracts from one or two or more herb medicines selected from the group consisting of Nepetae Herba, Myricae Cortex, etc., are blended.

Description

[Detailed description of the invention]

0001

FIELD OF THE INVENTION The present invention relates to a whitening cosmetic that is excellent in skin safety, has the effect of preventing skin darkening, and has the effect of rapidly lightening dark skin.

0002

[Prior Art and Problems to be Solved by the Invention] In general, darkening caused by sunburn occurs when tyrosine present in the skin is oxidized and metabolized to melanin, so it is impossible to prevent this oxidation. It is considered essential for preventing skin darkening, age spots, and freckles.

[0003] As substances having such an antioxidant function, substances that capture and eliminate active oxygen and substances that inhibit the activity of tyrosinase, an enzyme involved in melanin biosynthesis, are known to be effective. There is.

For example, JP-A-55-87712 proposes a cosmetic containing superoxide dismutase (SOD), which captures and eliminates active oxygen. However, the storage stability of this product was not sufficient, and there was a drawback that it was poor in practical use.

[0005] In addition, as a substance having a tyrosinase activity inhibiting effect, a derivative of vitamin C (Japanese Unexamined Patent Publication No. 56-13
5411), hydroquinone derivatives (Japanese Unexamined Patent Publication No. 1983)
-56912)) have been proposed. However, these whitening cosmetics either have insufficient storage stability or do not have sufficient whitening effects, or they have the effect of lightening dark skin, but they do not work well on the skin. This poses a safety problem, and it has been difficult to obtain a skin-whitening cosmetic that is excellent enough to have a positive whitening effect.

[0006] As described above, the reality is that no whitening cosmetic has been obtained that has excellent skin safety and simultaneously has the effect of preventing skin darkening and the effect of quickly lightening dark skin. Met.

The present invention was developed in view of the above circumstances, and has the effect of preventing skin darkening without causing skin irritation, that is, the effect of suppressing melanin pigment formation, and the ability to quickly lighten dark skin. The object of the present invention is to provide a whitening cosmetic that simultaneously has a skin-lightening effect.

[0008]

[Means for Solving the Problems] In order to achieve the above object, the whitening cosmetic of the present invention comprises one or more selected from the group consisting of It is characterized by containing extracts obtained from two or more kinds of herbal medicines.

[0009] In order to obtain the crude drug extract applicable to the present invention as described above, for example, the following method can be suitably used. That is, one or more herbal medicines selected from the group consisting of ▲ka▼, 芊薼, fried plum peel, and 樸▲soku▼ are mixed with 5 to 30 times their weight of water or lower alkyl alcohol, such as methanol. The desired extract is obtained by boiling or cold soaking with a solvent such as , ethanol, n-propanol, n-butanol, etc., then concentrating the obtained extract by filtration, removing water or alcohol under reduced pressure, and drying. It will be done. Further, the above water or alcohol extract may be used after being purified using a column or the like.

[0010] The above-mentioned method represents one embodiment of the method for obtaining the herbal medicine extract applied to the present invention, and the method used in the extraction process in the manufacturing process of the whitening cosmetic according to the present invention. The solvent and the order in which the solvent is used are not limited to these and can be arbitrarily selected.

[0011] Further, the amount of the above extract to be blended in the present invention is preferably 0.005 to 2.0% by weight (hereinafter abbreviated as wt%) based on the total weight of the whitening cosmetic composition of the present invention.
More preferably, it is 0.01 to 1.0 wt%. That is,
If the amount is less than 0.005 wt%, the whitening effect obtained will not be sufficient, and if it exceeds 2.0 wt%, no effect commensurate with the increased amount can be expected.

[0012]The whitening cosmetic of the present invention was able to exhibit excellent effects in that the above-mentioned extract acts on the skin, suppressing the production of melanin and lipid peroxide, and preventing pigmentation.

The whitening cosmetic composition of the present invention can be applied to lotions, milky lotions, creams, packs, etc., for example.

[0014] In addition to the above, pigments, fragrances, preservatives, surfactants, pigments, etc. can be appropriately incorporated into the whitening cosmetic of the present invention within the range that achieves the object of the present invention.

[0015]

[Examples] The present invention will be explained in detail below based on Examples and Comparative Examples. In addition, ■ skin irritation test described in Examples,
■Active oxygen elimination test, ■Tyrosinase activity inhibition rate measurement test, ■Skin color brightness recovery test, and ■Whitening practical test are shown below.

■Skin irritation test 0.05 g of sample was applied to the skin of the flexor side of the forearm of 25 subjects who were exposed to summer sunlight for 6 hours (3 hours a day for 2 days) using a circular linen cloth with a diameter of 1.0 cm. After 24 hours of occlusion using a cloth-attached patch test bandage, the condition of the skin of 25 test subjects was evaluated for each sample according to the following criteria. The results of the evaluation were determined based on the stronger reaction between 1 hour and 24 hours after removal of the bandage, and were expressed as the number of people with an evaluation of (±) or higher as shown in Table 1.

[0017]

[Table 1]

■ Active oxygen scavenging test 0.05 mol sodium carbonate solution (pH 10.2) 2
．． 4 ml, 3 mmol xanthine 0.1 ml, 3 mmol ethylenediaminetetraacetate 0.1 ml
, 0.15% calf serum albumin 0.1ml, 0.75
Millimole Nitro Blue Tetrazolium 0.1ml
, add 0.1 ml of the sample solution, and pre-incubate at 25°C for 10 minutes.

[0019] This was supplemented with 0.17 units/ml of xanthine.
After adding 0.1 ml of oxidase and heating at 25°C for 20 minutes, the absorbance at a wavelength of 560 nm (
T1) was measured. On the other hand, the absorbance (T2
), absorbance of sample solution with 0.1 ml of water added (T3), sample solution, absorbance of sample solution with 0.2 ml of water added instead of xanthine oxidase (T4)
) were measured, and the inhibition rate (%) was calculated using the following formula. As a result, evaluation was made according to the following classification. Those showing an inhibition rate of 85% or more: ○〃60
% or more and less than 85%: △〃 Less than 60%
:-

■Tyrosinase activity inhibition rate measurement test The measurement principle of this test is to measure dopachrome produced by the action of tyrosinase on tyrosine. The reaction composition liquid was L-tyrosine (0.3 mg
/ml) 1.0ml, McCleben buffer (pH 6.8
), 1.0 ml of dehydrodieigenol, 0.9 ml of dehydrodieugenol were prepared in a total volume of 2.9 ml, incubated at 37°C for 10 minutes, and 0.1 ml of tyrosinase (1 mg/ml, mushroom, manufactured by Sigma) was added. ℃，
Incubation was performed for 15 minutes, and absorbance at 475 nm was measured. The above dehydrodieugenol is difficult to dissolve in water, so it was dissolved in 1% ethanol to a final concentration of 0.
．． Samples of 6, 1.5, and 3.0 mM were prepared and used. In addition, since it is possible that the enzyme was inactivated by ethanol, we also conducted a study using the same concentration to confirm that there was no enzyme inactivation. The inactivation of tyrosinase enzyme inhibitory activity was calculated using the following formula. (A): Adding buffer solution instead of sample (B): Adding sample (C): Adding buffer solution instead of tyrosinase (D):
As a result, adding buffer instead of tyrosinase and sample
Evaluation was made according to the following classification. Those showing an inhibition rate of 85% or more: ○〃60
% or more and less than 85%: △〃 Less than 60%
:-

■Skin color brightness recovery test The back skin of 20 test subjects was irradiated with ultraviolet rays in the UV-B region twice the minimum amount of erythema, and one week later, the irradiated areas were divided into sample application areas and non-application areas. were set, and the reference brightness (V0 value, V0' value) of each skin was measured. Subsequently, the sample was continuously applied to the application site once a day for 3 months.
After 8 and 13 weeks, the brightness (Vn value, Vn' value) of the skin at the application site and the non-application site was measured, and the recovery of skin color was evaluated according to the criteria shown in Table 2.

[0022] The brightness of the skin (Munsell color system V value) is calculated from the X, Y, Z values obtained by measuring with a high-speed spectrocolorimeter, and the evaluation was made after 13 weeks by 20 non-examiners. It is shown as the average value of evaluation points.

[0023]

[Table 2]

■Practical skin whitening test The skin on the flexor side of the forearm of 20 subjects who were exposed to sunlight for 3 hours (1.5 hours a day for 2 days) in the summer was tested. Starting from the day after exposure to sunlight, and 7 days after exposure to sunlight, the samples were applied once in the morning and once in the evening for 13 weeks on the flexor side of the right forearm. This evaluation was expressed by the number of subjects who answered that the skin area to which the sample was applied was fairer (light-skinned) than other skin areas.

Example 1 (Two-Layered Lotion) A two-layered lotion was prepared by blending the Equinox roe extract prepared as described below as shown in Table 3.

(Preparation of ▲Ka▼ roe extract) Dried ▲Ka▼
1500 ml of water was added to 100 g of the seed powder, boiled for about 1 hour, filtered to obtain an extract, and further concentrated and freeze-dried to obtain 35.0 g of a powdered extract.

[0027]

[Table 3]

(2) Preparation method After uniformly dissolving the above component (B) in component (C), component (A) and component (C) are uniformly mixed and dispersed,
Containers were then filled. At the time of use, the contents were uniformly dispersed by shaking.

(3) Properties The properties are shown in Tables 4 to 7. As is clear from Tables 4 to 7, the properties of the two-layer lotion of the present invention were excellent.

[0030]

[Table 4]

[0031]

[Table 5]

[0032]

[Table 6]

[0033]

[Table 7]

Example 2 [Two-layered lotion] (Preparation of Physcomitrium extract) 16.0 g of Physcomitrella extract was obtained in the same manner as in Example 1, except that Physcomitrella nigra was used instead of ▲ or ▼. Then, a two-layered lotion of the present invention was obtained in the same manner as in Example 1, except that this Apertica extract was used in place of the Aperture extract. The characteristics are shown in Tables 4 to 7. As shown in Tables 4 to 7, the two-layer lotion according to the present invention had excellent properties.

Example 3 [Two-layered lotion] (Preparation of fried plum peel extract) A fried plum peel extract 22. 5g was obtained. Then, a two-layered lotion of the present invention was obtained in the same manner as in Example 1, except that this fried plum peel extract was used instead of the roe extract. The characteristics are shown in Tables 4 to 7. Table 4
~As shown in Table 7, the properties of the two-layer lotion according to the present invention were excellent.

Example 4 [Two-layered lotion] (Preparation of 樸▲soku▼ extract) A 樸▲soku▼ extract was prepared in the same manner as in Example 1 except that 樸▲soku▼ was used instead of ▲ or roe. Things 8.7
I got g. And instead of ▲ or ▼ roe extract, use this
A two-layer lotion of the present invention was obtained in the same manner as in Example 1, except that the extract was used. The characteristics are shown in Tables 4 to 7. As is clear from Tables 4 to 7, the properties of the two-layer lotion according to the present invention were excellent.

Examples 5 to 8, Comparative Examples 1 to 3 [Skin cream] In the same manner as in Example 1, each skin cream was prepared with the composition shown in Table 8 and various tests were conducted, and the results are shown in Tables 4 to 3. It is shown in Table 7.

[0038]

(2) Preparation method Component (B) is uniformly dissolved in component (A) and then heated to 80°C.
heated to. On the other hand, after heating and melting the component (C) at 80°C, and then injecting and stirring the component (C) into the component (A),
The mixture was cooled to a temperature of 30° C. while stirring.

(3) Properties As shown in Tables 4 to 7, the skin creams of Examples 5 to 8 according to the present invention showed good results in all tests.

On the other hand, as is clear from Comparative Examples 1 to 3, skin creams containing no crude drug extracts or containing 0
．． Skin creams containing less than 0.005% were inferior in various properties.

Furthermore, as is clear from Comparative Example 4, the skin cream containing arbutin, a hydroquinone derivative, was also inferior in various properties.

[0043]

[Effects of the Invention] As described above, it is clear that the present invention provides a useful whitening cosmetic that does not cause skin irritation and is excellent in suppressing melanin pigment formation and rapidly lightening dark skin. It is.

Claims (1)

[Claims] [Claim 1] ▲Ka▼ roe, jade, fried plum peel, birch ▲soku▼
A whitening cosmetic characterized by containing an extract obtained from one or more crude drugs selected from the group consisting of: