JPH0995436A - Preparation for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an external preparation for skin, having an excellent melamine production-inhibiting action, an excellent tyrosinase activity-inhibiting action, an excellent esterase-inhibiting action and an antioxidizing action. SOLUTION: This preparation for skin contains the extract of a Myrtaceae Baeckea plant (e.g. jongrahab) in an amount of 0.0005-20wt.%, preferably 0.001-10wt.%, as the dry product. The extract is obtained by immersing or thermally refluxing the leaves, stems, fruits, whole body, etc., of the Baeckea plant in an extraction solvent (e.g. ethanol), filtering the product, and subsequently concentrating the filtrate. The external preparation for the skin exhibits excellent paling effects to beautifully whiten chromatosis, spots, ephelis, chloasma, etc., after sunburnt, recover and maintain the tension and elasticity of the skin to prevent the skin from aging, and maintain the young skin. The preparation can express preventing effects on the oxidation of skin lipids and curing the oxidation injuries of the skin to protect the skin. The preparation can further be applied to make-up cosmetics, foundation cosmetics, toiletry products, etc.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD [0001] The present invention suppresses the production of melanin by incorporating an extract of a specific plant, and is effective for preventing and improving pigmentation, spots, freckles, melasma, etc. after sunburn. It has an activity-inhibiting effect and has an excellent effect on whitening the skin, and by suppressing the activity of elastase to restore and maintain the firmness and elasticity of the skin, it prevents skin aging and leaves youthful skin. The present invention relates to an external preparation for skin, which can maintain the above-mentioned properties, and exhibits its effectiveness in preventing the oxidation of lipid components in the skin and in oxidative damage to the skin.

[0002]

BACKGROUND OF THE INVENTION Although there are some unclear points about the mechanism of the generation of skin spots and the like, it is generally caused by hormonal abnormalities and irritation of ultraviolet rays from sunlight. It is believed that melanin pigment is formed, which is abnormally deposited in the skin. This melanin pigment, which causes skin coloring, is produced in the melanin-producing granules (melanosomes) in the melanocytes (melanocytes) between the epidermis and the dermis, and the produced melanin diffuses into adjacent cells by osmotic action. . The biochemical reaction in this melanocyte is presumed to be as follows.

That is, tyrosine, which is an essential amino acid, is converted into dopaquinone by the action of the enzyme tyrosinase, which is converted into black melanin via a red pigment and a colorless pigment by an enzymatic or non-enzymatic oxidative action in the process of producing melanin pigment. is there. Therefore, suppressing the action of tyrosinase, which is the first step of the reaction, is important for suppressing melanin production.

However, the compounds that suppress the action of tyrosinase, except for hydroquinone, exhibit their effects very slowly, so that the effect of improving skin pigmentation is not sufficient. On the other hand, although hydroquinone is recognized as having an effect, its use is generally restricted because of its sensitizing properties. In order to improve the safety, attempts have been made to use monoesters or alkyl monoethers of higher fatty acids (Japanese Patent Application Laid-Open No. 58-154507), but the esters are decomposed by hydrolytic enzymes in the body. For this reason, it is not always safe to say, and ethers have not been obtained that are sufficiently satisfactory in terms of safety.

On the other hand, in recent years, research on aging has been advanced, and as a cause of skin aging, aging is an important factor from a macroscopic viewpoint, and further, drying, oxidation, and sunlight (ultraviolet rays).
The effects of the above have also been cited as direct factors related to skin aging. As a concrete phenomenon of skin aging,
It is known that collagen and elastin are reduced in the dermis of the skin, mucopolysaccharides such as hyaluronic acid are reduced, and cell damage by ultraviolet rays is caused. Of these, elastin forms a cross-link with each other and contributes to the elasticity of tissues.However, elastin, which is an elastin-disrupting enzyme, is overexpressed by UV exposure and aging, and elastin is denatured and destroyed. However, it is thought to lead to a decrease in skin elasticity. Therefore, it is important to prevent aging of the skin by suppressing the action of elastase and preventing the degeneration and destruction of elastin which gives elasticity and firmness to the skin.

Further, various antioxidants have been conventionally added to cosmetics for the purpose of improving shelf life, but synthetic antioxidants such as BHA (butylhydroxyanisole) and BHT (butylhydroxytoluene) are particularly safe. Their use is being re-examined from a sex perspective. Against this background, the demand for vitamin E as a natural antioxidant is increasing, but its antioxidant effect is weak, and there are problems with the source, effect, solubility, price, etc. In addition, at present, there is a strong demand for the development of new antioxidants that are effective in preventing skin lipid components from being oxidized, oxidizing skin damage, and skin aging by being incorporated into cosmetics and the like. .

[0007]

In view of the above situation, the present inventors have examined a wide variety of substances for their melanin production inhibitory action, tyrosinase inhibitory action, elastase inhibitory action and antioxidant action. Futomomo (My
rtaceae) Beckea (Baeckea) plant extract has excellent melanin production inhibitory action, tyrosinase inhibitory action,
They found that they have an elastase inhibitory action and an antioxidant action, and completed the present invention. There have been no reports of melanin production-inhibiting activity or tyrosinase-inhibiting activity of the Betkea (Baeckea) family of the family Myrtaceae, as well as its application to whitening agents, anti-aging agents, and antioxidants, as well as to skin external preparations. The application of is not known at all. The present inventors have completed the present invention based on the above findings.

That is, the present invention is directed to Myrtaceae (Myrtaceae).
) An external preparation for skin characterized by being mixed with an extract of a plant belonging to the genus Beckea. The external preparation for skin of the present invention is preferably a whitening agent, an anti-aging agent or an antioxidant, and the anti-aging agent is preferably an elastase inhibitor among them.

The structure of the present invention will be described in detail below. The plant of the genus Beckea belonging to the family Myrtaceae used in the present invention is Jon Grave (Jon).
Grahab, scientific name: Baeclea frutescens) is suitable. These are plants that grow especially on dry grasslands and pastures in Indonesia.

Fusarium peach (Myrtaceae) used in the present invention
) The family Beckea (Baeckea) genus plant extract, leaves, stems including rhizomes, fruits, etc.
After immersing the whole plant with the extraction solvent or heating to reflux,
Obtained by filtration and concentration. More specifically, the plant of the genus Beckea (Baeckea) belonging to the family Myrtaceae is used as a solvent,
For example, extract with a polar solvent such as water, lower alcohol, hydrous lower alcohol, propylene glycol, 1,3-butylene glycol, butanol or an organic solvent of chloroform, dichloroethane, carbon tetrachloride, acetic acid ethyl ester, ether or a mixture thereof. The extract obtained as described above is used as it is or as a concentrated extract, or the extract is subjected to an adsorption method, for example, impurities are removed using an ion exchange resin, or a porous polymer (for example, Amberlite X
It is also possible to use an extract that has been adsorbed on a column of AD-2) and then eluted with methanol or ethanol and concentrated. Further, a partitioning method, for example, an extract extracted with butanol or the like is also used.

The amount of the extract of the plant of the genus Beckea belonging to the family Myrtaceae belonging to the present invention is 0.0005 to 20.0% by weight, preferably 0.001 to 0.001% by weight as a dry product in the total amount of the external preparation for skin. It is 10.0% by weight.
If the amount is less than 0.0005% by weight, the effects of the present invention cannot be sufficiently exerted, and if it exceeds 20.0% by weight, it is difficult to formulate the composition, which is not preferable. Further, even if the content is 10.0% by weight or more, the effect is not so much improved.

The external preparation for skin of the present invention contains, in addition to the above-mentioned essential components, components usually used in external preparations for skin such as cosmetics and pharmaceuticals, for example, other whitening agents, moisturizers, antioxidants,
An oily component, an ultraviolet absorber, a surfactant, a thickener, an alcohol, a powder component, a coloring material, an aqueous component, water, various skin nutritional agents and the like can be appropriately blended as necessary.

In addition, sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice extraction Substance, glabridin, hot water extract of karin fruit, various herbal medicines, drugs such as tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbyl phosphate, ascorbyl glucoside, arbutin, kojic acid, etc. Whitening agents, glucose, fructose, mannose,
Sugars such as sucrose and trehalose can also be appropriately blended.

INDUSTRIAL APPLICABILITY The present invention can be widely applied to cosmetics applied to the outer skin, quasi-drugs, and the like, particularly preferably to cosmetics, and the dosage form thereof is an aqueous solution system, a solubilization system, or an emulsion system. , A powder type, an oil liquid type, a gel type, an ointment type, an aerosol type, a water-oil two-layer system, a water-oil-powder three-layer system, etc. That is, for basic cosmetics, facial cleanser, lotion,
It is widely applicable to various forms such as emulsion, cream, gel, essence (cosmetic liquid), pack, mask and the like. In addition, if it is makeup cosmetics,
As toiletries such as foundations, it can be widely applied in the form of body soap, soap, etc. Furthermore, quasi-drugs can be widely applied to various forms such as ointments. And, in these dosage forms and forms,
The form that the external preparation for skin of the present invention can take is not limited.

[0015]

Next, the present invention will be described in more detail by way of examples. The present invention is not limited to this. The blending amount is% by weight. Prior to the examples, test methods and results of the melanin suppressing effect, tyrosinase activity inhibiting effect and whitening effect, elastase inhibiting effect, and antioxidant effect of the plant extract of the present invention will be described.

Test method for melanin suppressing effect, tyrosinase activity inhibiting effect, and whitening effect and the results 1. Sample Preparation (1) Jongrahab Extract Whole plant of Jongrahab 50
g was immersed in ethanol at room temperature for 3 days, and the extract was concentrated to obtain 10.9 g of a methanol extract. This extract was dissolved in DMSO at 1%, the concentration was adjusted by diluting this solution, and the following experiment was performed using this.

2. Cell culture method B16 melanoma cultured cells derived from mice were used. 1
0% FBS and theophylline (0.09 mg / ml)
Was cultured in a CO2 incubator (95% air, 5% carbon dioxide) at 37 ° C. in an Eagle MEM medium containing After 24 hours of culturing, the sample solution was added so that the final concentration (concentration of extracted dry matter) was 10 −2 to 10 −5% by weight, and the culturing was continued for another 3 days. The determination and the tyrosinase activity inhibitory effect were measured.

3. Visual measurement of melanin amount A diffuser plate was placed on the lid of the plate of the well, the intracellular melanin amount was observed with an inverted microscope, and compared with the case of the sample (standard) to which the plant extract was not added. Table 1 shows the results.
Displayed in. In addition, as a reference example, the same test as above was carried out on an extract of Kaigai (Lamiaceae, Odorikosou Subfamily), which is already known to have an action of suppressing melanin production. Table 1 also shows the results. Further, in the table, "toxic" means that it is cytotoxic.

<Judgment Criteria> 白: White (melanin content) Δ: Slightly white (melanin content) ×: Standard (melanin content)

4. Measurement of tyrosinase activity Before measurement, the medium in the wells is removed and washed twice with 100 μl of PBS. 45 μl of 1% Triton-X in each well
(Rohm and Haas product name, surfactant) containing PBS is added. Shake the plate for 1 minute, break the cell membrane well, and use a microplate reader for 475n.
The absorbance at m was measured, and this was taken as the absorbance at 0 minutes. Then, 5 μl of 10 mM L-DOPA solution was quickly added, and the mixture was transferred to an incubator at 37 ° C. and reacted for 60 minutes. The plate was shaken for 1 minute, and the absorbance (475 nm) at 60 minutes was measured. The tyrosinase activity ratio (%) was defined as the ratio of the absorbance difference of the sample with the plant extract to the absorbance difference between the time of 0 minute and the time of 60 minutes in the case of the sample to which no plant extract was added (control). Table 1 shows the results. Further, as a reference example, the same test as above was carried out on an ethanol extract of mussel, which is already known to have a tyrosinase activity inhibitory action. Table 1 also shows the results. In the table, "toxic" means that cytotoxicity was observed, and "-" means that no significant difference was observed within a risk rate of 5% as compared with the control.

[0021]

[Table 1] ──────────────────────────────────── Test melanogenesis visual evaluation Tyrosinase activity rate ( %) ──────────────────────────────────── Concentration (wt%) 10-5 10-4 10 -3 10-2 10-5 10-4 10-3 10-2 ───────────────────────────────── ─── Zhonggurab extract △ △ ○ Toxicity − − 39 Toxicity Kaigai extract × × × × − − − − 55 ───────────────────────── ─────────────

5. Whitening test [Test method] 40 subjects exposed to sunlight in summer for 4 hours (2 hours per day for 2 days) each sample from 5 days after the day of sun exposure to the skin of the upper arm inner skin Was applied once each morning and evening for 4 weeks. The panel was divided into 8 groups, and 5 groups were prepared, and the tests were conducted according to the formulations shown below. (Alcohol phase) 95% ethyl alcohol 55.0% by weight Polyoxyethylene (25 mol) hydrogenated castor oil ether 2.0 Antioxidant / preservatives Appropriate amount Perfume Appropriate amount Drug (Table 2) (Aqueous phase) Glycerin 5.0 Sodium hexametaphosphate Appropriate amount Ion-exchange water Residue <Production method> Prepare an aqueous phase and an alcohol phase, respectively, and then mix and solubilize both.

[Evaluation Method] The lightening effect after use was judged based on the following judgment criteria. <Judgment criteria> ：: When the ratio showing significant effect and effectiveness among the subjects is 80% or more 割 合: The ratio showing significant effect and effectiveness among the subjects is 50% or more
Less than 80% △: The proportion of the test subjects showing excellent and effective is 30% or more
When less than 50% x: When the ratio of markedly effective or effective among the subjects is less than 30%

Samples having the blending composition described in the above test method were prepared and the whitening effect was compared using the agents shown in Table 2.
The results are shown in Table 2.

[0025]

[Table 2] ────────────────────────── Drug compounding amount (% by weight) Effect ────────── ──────────────── Additive- × Hydroquinone 1.0 △ Jungularbe extract 0.1 ○ Jonglarbe extract 1.0 ○ Jeongular extract 10.0 ◎ ─────────────────────────

The john grave extract in Table 2 was obtained by heating and reducing whole grass of a plant, filtering, concentrating and drying the whole plant.

As is clear from Table 2, the effect after exposure to sunlight was confirmed that the addition of the john grave extract prevented excessive deposition of melanin pigment and prevented darkening. Was given.

Test Method for Elastase Inhibitory Activity and Results 1. Sample preparation Whole plant of Jongrahab 50
g was immersed in methanol at room temperature for 4 days and the extract was concentrated to obtain 4.28 g of a methanol extract. This extract was dissolved in DMSO at 1%, the solution was diluted with a reaction buffer to adjust the concentration to 100 ppm, and the following experiment was carried out using this.

2. Test method of elastase inhibitory activity and its results Elastase activity was measured according to the method of Fujie et al. In addition, as a reaction buffer, 0.1M
Performed using HEPES, 0.5 M NaCl, pH 7.4. Methoxy-succinyl as an elastase substrate
-alanyl-alanyl-prolyl-valine-p-nitroanilide (BA
CHEMFEIN CHEMIKALIEN AG)
It was dissolved in DMSO so as to have a concentration of 80 mM, dispensed in 20 μl aliquots, and stored frozen (−80 ° C.). At the time of use, it was diluted with a reaction buffer solution to 8 mM before use.

Elastase is an elastase derived from human leukocytes (ELASTIN PRODUCT CO., IN).
C.) and dissolved in the reaction buffer at 200 μg / ml, dispensed in 10 μl aliquots and stored frozen (-80
℃). At the time of use, it was diluted with the reaction buffer to 5 μg / ml before use. 96-well plate (CORN
ING 25860), 25 μl each of 8 mM elastase substrate was dispensed, and 50 μl of the inhibitor (concentration: 100 ppm) was further added. Next, 5 μg /
25 μl of elastase was added thereto, and the mixture was immediately incubated at 37 ° C. for 20 minutes. After that, 415 nm
The absorbance was measured with. However, the inhibition rate depends on the following function.

[0031]

## EQU1 ## Inhibition rate (%) = 100- (in the presence of inhibitor / no inhibitor) × 100

The results are shown in Table 3. Further, as a reference example, the same test as above was carried out on a soybean extract (trade name Elhibin; manufactured by Pentafarm Co.), which is already known to have elastase inhibitory activity. The results are also shown in Table 3.

[0033]

[Table 3]

Test method for antioxidant activity and its results 1. Preparation of Samples (1) Preparation Example 1 (Methanol Extract) Dried Seed Product 1 of Jongrahab 1
20 kg of methanol was added to kg, the mixture was immersed at room temperature for 10 days for extraction, the extract was filtered, and then methanol was concentrated to obtain 62 g of the extract as a dry residue.

(2) Preparation Example 2 (Chloroform Extract) 1 k of dried leaves of Jongrahab
Chloroform 9 kg was added to g, refluxed at 50 ° C. for 8 hours for extraction, the extract was filtered, and then chloroform was concentrated to obtain 42 g of the extract as a dry residue.

(3) Preparation Example 3 (Purified product of ethanol extract) Dried root of Jongrahab 1k
After adding 10 kg of ethanol to g and immersing for 10 days at room temperature for extraction, the extract was filtered, and the filtrate was washed with Amberlite XAD2 column with water and then eluted with ethanol to concentrate the eluate to obtain 18 g of extract.

2. Test method and test result 1.0 mg methyl linoleate (manufactured by Tokyo Kasei Kogyo Co., Ltd.) / Ml Acetone solution (1.0 ml) was added with 0.001 mg or 0.1% of the dried seed of Jongrahab prepared in Preparation Example 1. After adding 01 mg and dispensing and drying in a test tube with a screw lid, the oxidized group was treated at 50 ° C for 4 hours, and the non-oxidized group was stored at 4 ° C. After treatment, non-aqueous TBA
Reagent (0.12% 2-thiobarbituric acid (TBA)
0.50% toluethylamine (TEA) / acetic acid) 3.0
ml was added and reacted at 95 ° C. for 1 hour. Immediately after the reaction, the mixture was cooled with water, the absorbance at 532 nm was measured, and the oxidation inhibition rate was calculated.

[0038]

[Equation 2] Oxidation inhibition rate (%) = [1- (As50-As4)
/ (Ac50-Ac4)] × 100

Ac50: control Absorbance at 50 ° C. heating. Ac4: control Absorbance stored at 4 ° C. As50: Absorbance of sample heated at 50 ° C. As4: Absorbance of sample stored at 4 ° C.

As a control, Jon Grave (Jon
Vitamin E (DL) instead of dried seeds of
-Α-tocopherol, manufactured by Tokyo Chemical Industry Co., Ltd.), B
The same operation was performed for the one to which HT (manufactured by Wako Pure Chemical Industries, Ltd.) was added. The results are shown in Table 4.

[0041]

[Table 4] ────────────────────────────────── Sample No. Antioxidant Compounding amount (mg) Oxidation Inhibition rate (%) ─────────────────────────────────── 1 section grabber seed extract 0.001 60.3 2 section grabber Seed extract 0.01 93.0 ────────────────────────────────── 3 Vitamin E 0.001 42.4 4 Vitamin E 0.01 82.4 5 BHT 0.001 97.3 ───────────────────────────────────

As shown in Table 4, John Grave (Jon
The seed extract of (grahab) exhibits an antioxidant effect superior to that of vitamin E, and when the concentration is further increased, it exhibits a remarkable antioxidant effect equivalent to that of BHT.

Examples of blending various types of external preparations for skin according to the present invention will be described below as examples.

Example 1 Cream (Formulation) Stearic acid 5.0% by weight Stearyl alcohol 4.0 Isopropyl myristate 18.0 Glycerin monostearate 3.0 Propylene glycol 10.0 Jung Grab methanol extract 0.01 Caustic potash 0.2 Sodium bisulfite 0.01 Preservative Suitable amount Perfume Suitable amount Ion-exchanged water Residual (production method) Dissolve propylene glycol, sequan-methanol extract and caustic potash in ion-exchanged water and heat to 7
Keep at 0 ° C. (aqueous phase). Mix other ingredients and heat to melt
Keep at 0 ° C. (oil phase). The oil phase is gradually added to the water phase, and after the addition is completed, the temperature is maintained for a while to cause a reaction. Then, it is uniformly emulsified with a homomixer and cooled to 30 ° C. with thorough stirring.

Example 2 Cream (Formulation) Stearic acid 2.0% by weight Stearyl alcohol 7.0 Hydrogenated lanolin 2.0 Squalane 5.0 2-Octyldodecyl alcohol 6.0 Polyoxyethylene (25 mol) cetyl alcohol ether 3.0 Glycerin monostearate ester 2.0 Propylene glycol 5.0 John Grab methanol extract 0.05 Sodium hydrogen sulfite 0.03 Ethylparaben 0.3 Perfume suitable amount Ion-exchanged water Residue (production method) Propylene glycol in ion-exchanged water And add
Heat and maintain at 70 ° C. (aqueous phase). The other ingredients are mixed, heated and melted and maintained at 70 ° C. (oil phase). The oil phase is added to the aqueous phase to carry out preliminary emulsification, and the mixture is uniformly emulsified with a homomixer and then cooled to 30 ° C. with thorough stirring.

Example 3 Cream (Formulation) Solid paraffin 5.0% by weight Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) sorbitan monolaurate 2 0.0 Soap powder 0.1 Borax 0.2 John Grab methanol extract 0.05 John Grave ethanol extract 0.05 Sodium hydrogen sulfite 0.03 Ethyl paraben 0.3 Perfume proper amount Ion exchange water Residual (production method) Ion exchange water Soap powder and borax are added to the mixture, heated and melted and kept at 70 ° C (aqueous phase). The other ingredients are mixed, heated and melted and maintained at 70 ° C. (oil phase). The oil phase is stirred into the water phase and gradually added to carry out the reaction. After the reaction is completed, the mixture is uniformly emulsified with a homomixer, and after emulsification, the mixture is cooled to 30 ° C. with thorough stirring.

Example 4 Emulsion (formulation) Stearic acid 2.5 wt% Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3. 0 triethanolamine 1.0 carboxyvinyl polymer 0.05 (trade name: Carbopol 941, BFGoodrich Chemical company) John Grave acetic acid ethyl ester extract 0.01 sodium bisulfite 0.01 ethylparaben 0.3 perfume proper amount ion exchange Water Residue (Production Method) A carboxyvinyl polymer is dissolved in a small amount of ion-exchanged water (phase A). Polyethylene glycol 1500 and triethanolamine are added to the remaining ion-exchanged water, dissolved by heating, and kept at 70 ° C. (aqueous phase). The other ingredients are mixed, heated and melted and maintained at 70 ° C. (oil phase). The oil phase is added to the water phase to perform preliminary emulsification, the phase A is added, and the mixture is uniformly emulsified with a homomixer. After the emulsification, the mixture is cooled to 30 ° C. while stirring well.

Example 5 Emulsion (Formulation) Microcrystalline wax 1.0 wt% Beeswax 2.0 Lanolin 20.0 Liquid paraffin 10.0 Squalane 5.0 Sorbitan sesquioleate 4.0 Polyoxyethylene (20 mol ) Sorbitan monooleate 1.0 Propylene glycol 7.0 John Grave acetone extract 10.0 Sodium hydrogen sulfite 0.01 Ethylparaben 0.3 Fragrance appropriate amount Ion-exchanged water Residual (production method) Add propylene glycol to ion-exchanged water ,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase is gradually added thereto, and the mixture is uniformly emulsified with a homomixer. After emulsification, cool to 30 ° C with good stirring.

Example 6 Jelly (formulation) 95% ethyl alcohol 10.0% by weight dipropylene glycol 15.0 polyoxyethylene (50 mol) oleyl alcohol ether 2.0 carboxyvinyl polymer 1.0 (trade name: Carbopol) 940, BFGoodrich Chemical company) Caustic soda 0.15 L-arginine 0.1 John Grab 50% ethanol aqueous solution extract 7.0 2-Hydroxy-4-methoxybenzophenone sodium sulfonate 0.05 Ethylenediaminetetraacetate ・ 3 sodium ・ 2 water 0.05 Methylparaben 0.2 Perfume Suitable amount Ion-exchanged water Residual (production method) Carbopol 940 is uniformly dissolved in ion-exchanged water, while sequan 50% ethanol aqueous solution extract and polyoxyethylene (50 mol) in 95% ethanol Ole luia The lucol ether is dissolved and added to the aqueous phase. Next, after adding other components, the mixture is neutralized with caustic soda and L-arginine to increase the viscosity.

Example 7 Beauty Serum (Formulation) (Phase A) Ethyl Alcohol (95%) 10.0% by Weight Polyoxyethylene (20 mol) Octyldodecanol 1.0 Pantotenyl Ethyl Ether 0.1 Jung Grab Methanol Extraction Substance 1.5 Methylparaben 0.15 (Phase B) Potassium hydroxide 0.1 (Phase C) Glycerin 5.0 Dipropylene glycol 10.0 Sodium hydrogen sulfite 0.03 Carboxyvinyl polymer 0.2 (trade name: Carbopol 940, BFGoodrich Chemical company) Purified water Residual (manufacturing method) Phases A and C are uniformly dissolved, and A is added to phase C
Add phase and solubilize. Next, after adding the phase B, filling is performed.

Example 8 Pack (Formulation) (Phase A) Dipropylene glycol 5.0% by weight Polyoxyethylene (60 mol) hydrogenated castor oil 5.0 (Phase B) John Grab methanol extract 0.01 Olive oil 5. 0 Tocopherol acetate 0.2 Ethylparaben 0.2 Perfume 0.2 (Phase C) Sodium hydrogen sulfite 0.03 Polyvinyl alcohol 13.0 (Saponification degree 90, degree of polymerization 2,000) Ethanol 7.0 Purified water Residual ( Manufacturing method) A phase, B phase, and C phase are uniformly dissolved, respectively.
Phase B is added to the phase to solubilize it. Next, this is added to the C phase and then filled.

Example 9 Solid Foundation (Formulation) Talc 43.1% by weight Kaolin 15.0 Sericite 10.0 Zinc white 7.0 Titanium dioxide 3.8 Yellow iron oxide 2.9 Black iron oxide 0.2 Squalane 8 0.0 isostearic acid 4.0 POE sorbitan monooleate 3.0 isocetyl octoate 2.0 john grave ethanol extract 1.0 preservative appropriate amount perfume appropriate amount (manufacturing process) Talc to black iron oxide powder components are thoroughly mixed with a blender. Then, an oily component of squalane to isocetyl octoate, a sequan ethanol extract, a preservative, and a fragrance are added thereto, and the resulting mixture is well kneaded and then filled into a container and molded.

Example 10 Emulsion type foundation (cream type) (Formulation) (Powder part) Titanium dioxide 10.3% by weight Sericite 5.4 Kaolin 3.0 Yellow iron oxide 0.8 Bengala 0.3 Black iron oxide 0.2 (Oil phase) Decamethylcyclopentasiloxane 11.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (Aqueous phase) Purified water 50.0 1,3-Butylene glycol 4.5 John Grave ethanol extract 1.5 Sorbitan sesquioleate 3.0 Preservative proper amount Perfume proper amount (Production method) After heating and stirring the aqueous phase, the powder portion thoroughly mixed and pulverized is added and treated with a homomixer. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

[0054]

As described above, the external preparation for skin of the present invention has an excellent melanin production inhibitory action, tyrosinase activity inhibitory action, elastase inhibitory action and anti-oxidant action. It has an excellent effect on lightening stains, freckles, melasma, and whitening, and also suppresses the activity of elastase to restore and maintain the firmness and elasticity of the skin, preventing skin aging and youthful skin. It also has the effect of maintaining the above condition. In addition, the external preparation for skin of the present invention also has an antioxidant effect, and by appropriately using it, it is possible to produce cosmetics and the like that are excellent in safety and stable against oxidation. In addition, by blending it in cosmetics, it prevents the oxidation of lipid components of the skin and oxidative damage to the skin,
It is also effective against skin aging and can protect the skin.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Reference number within the agency FI Technical display location A61K 35/78 ADS A61K 35/78 ADSC AED AEDC (72) Inventor Kiichiro Yano Kohoku Ward, Kanagawa Prefecture Shibaido Daiichi Research Center, Inc. 1050, Nippa-cho (72) Inventor Yoshihiro Yokokawa, Shibaido Daiichi Research Center, 1050, Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa (72) Inventor Naomi Tanaka, Yokohama-shi, Kanagawa Shiseido Daiichi Research Center Co., Ltd. 1050 Shinba-cho, Kohoku Ward (72) Inventor Eiichiro Yagi Inside Shiseido Daiichi Research Center 1050 Shinba-cho, Kohoku Ward, Yokohama City, Kanagawa Prefecture (72) Inventor Hirohiko Sakamoto Kanagawa Shiseido Daiichi Lisa Co., Ltd. 1050 Shinba-cho, Kohoku-ku, Yokohama-shi, Japan In the arch center

Claims (7)

[Claims] 1. The Betcare (Ba) family of the Myrtaceae family
eckea) An external preparation for skin, which comprises an extract of a genus plant as an active ingredient. 2. An extract of a genus Baeckea is Jongrahab, scientific name: Baeclea.
frutescens), The external preparation for skin according to claim 1. 3. The external preparation for skin according to claim 1, which is a whitening agent. 4. The external preparation for skin according to claim 1, which is an anti-aging agent. 5. The external preparation for skin according to claim 4, which is an elastase inhibitor. 6. The external preparation for skin according to claim 1, which is an antioxidant. 7. An external preparation for skin, wherein the external preparation for skin according to claim 1 is a cosmetic.