KR20220055757A - Cosmetic composition containing lemon myrtle extract and its manufacturing method - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a lemon myrtle extract and a method for manufacturing the same, and more particularly, relates to a lemon myrtle ethanol extract, a solvent fraction thereof, and effects thereof. According to the present invention, the cosmetic composition is harmless to the human and does not cause side effects.

Description

Cosmetic composition containing lemon myrtle extract and manufacturing method thereof

The present invention relates to a cosmetic composition containing a lemon myrtle extract and a method for preparing the same.

The skin is a membrane that covers the outside of the body and performs various physiological functions to protect the body from environmental factors such as various external stimuli, obstacles, and dryness, thereby protecting and regulating internal organs and other internal organs. do.

However, as we age, the secretion of various hormones that control metabolism decreases, and the function and activity of immune cells decreases, leading to a decrease in the biosynthesis of immune proteins and proteins necessary for the living body, and externally, destruction of the ozone layer. The normal function of the skin is weakened, aging is accelerated, and the complexion is deteriorated and the skin is wrinkled due to physical and chemical stimulation and stress caused by the increase of ultraviolet rays, free radicals and free radicals caused by environmental pollution such as

The skin is largely composed of three layers: the epidermis, dermis, and subcutaneous adipose tissue. The epidermis is divided from the outside to the inside into the stratum corneum, clear layer, granular layer, and basal layer. The stratum corneum, the outermost layer of the epidermis, is the most important structure of the skin barrier function and consists of non-nucleated flat corneocytes and SC intercellular lipids. The multi-lamellar lipid layer formed of intercellular lipids such as ceramide, cholesterol, and fatty acids synthesized by keratinocytes of the skin barrier maintained through the division and differentiation process of normal epidermal cells is a protective barrier to prevent moisture in the skin from evaporating. plays a role

Various types of lipids exist between the keratinocytes of the stratum corneum of the skin, and these lipids exist between the keratinocytes to prevent evaporation of skin moisture and function as a skin barrier to protect the skin from external stimuli or contamination.

In addition, in the cosmetic industry, a number of products using natural products have been developed to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and as consumers' response to cosmetics using natural ingredients increases, the value of development as a cosmetic raw material is increasing.

Accordingly, the need for the development of cosmetic products derived from natural products with excellent biosafety and skin barrier improvement effect is constantly emerging.

In particular, as the skip-care concept, which takes care of various skin concerns at once and skips unnecessary skin care steps, has recently emerged, interest in functional ingredients that can achieve complex effects with one substance is rapidly increasing. there is.

As an example, vitamin C acts as a powerful bio-antioxidant in the skin, blood and other tissues to prevent oxidation or denaturation of biocomponents such as proteins by free radicals and peroxides with high reactivity in the living body. In addition, vitamin C exhibits a pigmentation inhibitory effect by inhibiting the production of melanin.

As such, vitamin C is an easily accessible natural substance and has complex effects, so many attempts have been made to use it. It is considered a difficult ingredient to use.

Nevertheless, in order to take advantage of the high physiological activity of vitamin C, many studies have been conducted to stabilize vitamin C, and results are gradually coming out.

The present inventors provide a cosmetic composition derived from a natural product with high stability while exhibiting antioxidant and whitening effects similar to vitamin C under the background described above, thereby replacing or supplementing vitamin C with relatively poor stability. was intended to develop.

Korean Patent Registration No. 10-2140652

An object of the present invention is to provide a cosmetic composition comprising a lemon myrtle extract or a fraction thereof.

Another object of the present invention is to provide a cosmetic composition that exhibits excellent antioxidant activity and whitening activity, and has high stability.

Another object of the present invention is to provide a method for preparing the lemon myrtle extract and a fraction thereof.

One aspect of the present invention for solving the above problems provides a cosmetic composition comprising a lemon myrtle extract or a fraction thereof.

In the present invention, "lemon myrtle" is a plant of the family Myrtaceae, and the scientific name is Backhousia citriodora , lemon scented myrtle, lemon scented ironwood, sweet verbena. It is also called sweet verbena tree, sweet verbena myrtle, lemon scented verbena, lemon scented backhousia, etc. Lemon myrtle is native to the subtropical rain forests of central and southeastern Queensland, Australia.

In the present invention, the "extract" refers to a solvent in which the active ingredient contained in the extraction raw material is transferred by contacting the solvent and the extraction raw material under specific conditions. It can be included regardless of the type of For example, extracts of components soluble in solvents from natural products using water or organic solvents, specific components of natural products, for example, products obtained by extracting only specific components such as oil may be included. In addition, extracts of all formulations that can be formed using the extract itself and the extract, such as extracts of lemon myrtle, dilutions or concentrates of the extracts, dried products obtained by drying the extracts, preparations or purified products of the extracts, or mixtures thereof may include Furthermore, the extract may be extracted from natural, hybrid or variegated plants, and may also be extracted from plant tissue cultures.

The method of extracting the extract of the present invention may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include cold extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, extrusion extraction, and the like, and these may be performed alone or in combination of two or more methods.

The type of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used as needed. Non-limiting examples of the extraction solvent include water; C1-C4 lower alcohols, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Alternatively, a mixture thereof may be used, and specifically, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. In addition, the extract extracted as described above is filtered to remove floating solid particles, for example, filtered using nylon or the like, or filtered using freeze filtration, etc. It can be used after drying.

The "processing or treatment" may be, for example, additional fermentation or enzymatic treatment of the extract.

Accordingly, as used herein, an extract is a fraction, an extract or a crude or purified product of a fraction, or a dilution, concentrate, dried product, fermented product, or mixture thereof, and the like, including extracts of all formulations formable from lemon myrtle. am.

In the present invention, the “lemon myrtle extract” may be an extract of the whole herb, which is the leaf, flower, root, stem, and fruit of lemon myrtle, and a combination thereof, and may be an extract of a part or all of the above-ground part or the root part of the herb; Preferably, it may be an extract of lemon myrtle whole plant, and more preferably, it may be an extract of lemon myrtle leaves.

In the present invention, the lemon myrtle extract may be prepared using a general preparation method known in the art, and is not particularly limited. For example, ultrasonic extraction method, supercritical extraction method, vacuum extraction method reflux extraction method, hot water extraction method, low temperature hot water extraction method, high temperature pressure extraction method, low temperature high pressure extraction method, ultra high pressure extraction method, enzyme extraction method, solvent extraction method, cold extraction method, steam distillation method, low temperature high pressure distillation method, It may be prepared by a known method using an elution method, a compression method, a heat extraction method, or an extraction solvent, but is not limited thereto. In a preferred experimental example of the present invention, the lemon myrtle extract may be obtained by low-temperature high-pressure distillation.

In the present invention, the lemon myrtle extract may be prepared as an extract using water, an organic solvent, or a mixture thereof as an extraction solvent. The water may include, for example, distilled water, purified water, or sterile water, but is not limited thereto. The organic solvent may include, for example, alcohol, glycerin, butylene glycol, propylene glycol, methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane, but is not limited thereto.

In the present invention, the lemon myrtle extract is preferably extracted with a solvent selected from the group consisting of water, C _1-6 alcohol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, cyclohexane, and mixtures thereof. It may be extracted with a solvent. The concentration of the C _1-6 alcohol may be 10% to 90%, preferably 30% to 90%, more preferably 50% to 90%, most preferably 70% ethanol It may be extracted using .

The lemon myrtle extract of the present invention may be purchased commercially, or may be prepared by the known extraction method described above, and preferably extracted in distilled water at low temperature. In a preferred embodiment of the present invention, the lemon myrtle extract may be extracted using 70% ethanol.

Another aspect of the present invention provides a cosmetic composition comprising the lemon myrtle extract prepared by the above method.

Specifically, the cosmetic composition may have antioxidant and skin whitening effects, and each of these effects was confirmed through specific experimental examples.

In the cosmetic composition of the present invention, the content of the lemon myrtle extract may be 90 w/v% or less, preferably 80 w/v% or less, and more preferably 70 w/v% or less. In addition, in the cosmetic composition of the present invention, the content of the lemon myrtle extract may be 0.1 w/v% or more, preferably 5 w/v% or more, and more preferably 10 w/v% or more. In a specific embodiment of the present invention, the content of the lemon myrtle extract may be 10 to 70 w / v%.

In the present invention, the term “skin whitening” refers to suppressing the production of various biomolecules such as melanin, oxidation-reduction hemoglobin, carotene, and melanoids that affect skin color, thereby alleviating skin pigmentation phenomena such as blemishes, freckles, and melasma. It refers to the effect of improving skin brightness and uniformity by alleviating yellowness and redness. Specifically, in the present invention, skin whitening inhibits the activity of tyrosinase to suppress pigmentation, thereby brightening the skin tone and increasing the brightness. used to mean, but is not limited thereto.

In the present invention, the cosmetic composition comprises a softening lotion, a nutrient lotion, a moisture cream, a nourishing cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a mist, a spray and a powder. It can be formulated with one or more selected from. Specifically, the cosmetic composition may be formulated in the form of a mist for skin whitening and moisturizing, but is not limited thereto.

The cosmetic composition may be formulated by a conventional method. In the formulation of external skin preparations, reference may be made to the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, and in the formulation of cosmetic compositions, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association) , Inc., Washington, 1995) may be referred to.

Specifically, the cosmetic composition can be prepared in general emulsified formulations and solubilized formulations. For example, lotion, such as a softening lotion or a nourishing lotion; emulsions such as facial lotions and body lotions; creams such as nourishing creams, moisturizing creams, and eye creams; essence; makeup ointment; spray; gel; pack; sunscreen; makeup base; foundations such as liquid type, solid type or spray type; powder; makeup removers such as cleansing creams, cleansing lotions, and cleansing oils; Alternatively, it may be formulated as a cleaning agent such as a cleansing foam, soap, body wash, but is not limited thereto. In addition, the cosmetic composition may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

In the cosmetic composition, in addition to the essential components in each formulation, other components may be appropriately blended within the range that does not impair the purpose according to the present invention according to the type or purpose of use, etc. of the formulation.

The cosmetic composition may include a conventionally acceptable carrier, for example, oil, water, surfactant, humectant, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but is limited thereto not.

The acceptable carrier may vary depending on the formulation. For example, when formulated into an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or their Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component, and in the case of a spray, chlorofluorohydro It may further contain a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan may be used.

When the cosmetic composition is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

When the cosmetic composition is formulated as a soap, alkali metal salt of fatty acid, fatty acid hemiester salt, fatty acid protein hydrolyzate, isethionate, lanolin derivative, aliphatic alcohol, vegetable oil, glycerol, sugar, etc. can be used

The cosmetic composition is a fatty substance, organic solvent, solubilizer, thickening agent, gelling agent, softening agent, antioxidant, suspending agent, stabilizing agent, foaming agent, and fragrance commonly used in the industry depending on the quality or function of the final product. , surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetics or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

The cosmetic composition comprising the lemon myrtle extract of the present invention exhibits antioxidant and whitening effects as a complex function similar to vitamin C, is harmless and non-toxic to the human body derived from natural products, and does not cause side effects even when applied to the human body. The usability of the furnace is high.

In addition, the cosmetic composition of the present invention is characterized in that the stability is remarkably improved compared to vitamin C, and various additives and essential processes for stabilizing vitamin C can be omitted, thereby improving the cost and required time of the cosmetic manufacturing process. can do.

The cosmetic composition is used as an external preparation for skin such as suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, and spray by utilizing it. useful for

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 shows the lemon myrtle extract fractionation process and results (A: solvent fractionation process, B: fractionation result).
2 shows the results of confirming the antioxidant effect of lemon myrtle extract and its fractions through DPPH assay.
3 shows the results of confirming the antioxidant effect of lemon myrtle extract and its fractions through ORAC assay.
4 shows the results of confirming the tyrosinase inhibitory activity of a lemon myrtle extract and a fraction thereof.
5 to 7 show the results of confirming the melanin production inhibitory activity of the lemon myrtle extract and its fractions.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples only illustrate the present invention, and the present invention is not limited by the following examples.

Example 1. Preparation of Lemon Myrtle Extract and Fractions

In order to prepare the cosmetic composition of the present invention, freshly washed and dried lemon myrtle leaves were stirred and extracted using 70% ethanol at room temperature for 24 hours, and this was repeated three times. The extract was filtered through a 150 mesh and 0.5 μm filter, and the obtained extract was concentrated under reduced pressure at 40° C. using a vacuum rotary concentrator, and the resulting concentrate was washed with purified water, and then ethyl acetate ( Ethyl acetate), n-butanol (n-BuOH), and water (H 2 O) were used for solvent fractionation.

Experimental Example 1. Confirmation of antioxidant effect of lemon myrtle extract through DPPH assay

Active oxygen is a substance that is constantly produced in the normal metabolic process of the human body, and about 2 to 5% of the oxygen we breathe is converted into active oxygen. Free radicals cause strong oxidation and damage cells, proteins, and DNA, causing abnormalities in cell structure, function, and signal transduction system, and ultimately can cause inflammation in the body or induce aging.

In this experimental example, 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) scavenging activity was confirmed for each of the ethanol extract, ethyl acetate fraction, and n-butanol fraction of lemon myrtle through evaluation of the scavenging ability of active oxygen.

This experiment was commissioned by the Korean Dermatological Research Institute (Test No. KDRI-IV-2020-074), the sample was prepared by diluting it by concentration (0 to 400ug/mL), and 12.5 μL of the sample was added to 50 μL of ethanol. Then, 62.5 μL of 0.1 mM 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was added. After reacting for 30 minutes at 4° C. in the dark, absorbance was measured at 520 nm to confirm the concentration of DPPH radicals. The statistical significance of the results was confirmed through the t-test, and when the p value was less than 0.05, it was judged to be statistically significant.

As shown in the experimental results shown in FIG. 2 , it was confirmed that both the ethanol extract and the solvent fraction of lemon myrtle exhibited a concentration-dependent and statistically significant ( p <0.05) level of DPPH radical scavenging activity in the range of 3.13 to 400 μg/ml.

In particular, in the case of the lemon myrtle ethanol extract sample, at a concentration of 400 μg/ml, 85.99% of DPPH radical scavenging activity was shown (IC ₅₀ =5.27 μg/ml), and in the case of the lemon myrtle extract n -BuOH fraction at a concentration of 400 μg/ml, A DPPH radical scavenging activity of 86.94% was shown (IC ₅₀ =4.97 μg/ml). In the case of the lemon myrtle extract Ethyl aceatae fraction, 86.46% of DPPH radical scavenging activity was shown at a concentration of 400 μg/ml (IC ₅₀ =7.01 μg/ml), confirming the antioxidant effect.

That is, it was confirmed that the lemon myrtle extract and its fractions of the present invention exhibited DPPH radical scavenging activity of 50% or more and had excellent antioxidant activity.

Experimental Example 2. Confirmation of antioxidant effect of lemon myrtle extract through ORAC assay

Oxygen radical absorbance capacity (ORAC) assay is a test method mainly developed to confirm the antioxidant capacity of natural samples. One of the advantages of this ORAC assay is that it is suitable to check the antioxidant performance of a mixture in which various antioxidants are mixed. there is. This test method is a fluorescent molecule (beta-) that is oxidatively decomposed by mixing with a free radical former such as an Azo-initiator such as AAPH (2,2'-azobis(2-amidino-propane) dihydrochloride). This is a test to measure the amount of fluorescence of phycoerythrin or fluorescein). AAPH is a substance that generates radical peroxide by heat, and the radical peroxide decomposes the fluorescent substance to reduce the fluorescence. is measured using a fluorometer. When the oxidative decomposition process proceeds, the amount of fluorescence decreases during this process, and the change in the amount of fluorescence is measured for a certain period of time. The time-dependent curve of the fluorescence amount change obtained in this way, in particular, the area between the two curves depending on the presence or absence of an antioxidant corresponds to the antioxidant performance of the antioxidant. At the same time, check the inhibition rate of fluorescence change by concentration of the vitamin E derivative Trolox, a standard antioxidant, and create a standard curve for inhibiting fluorescence change by Trolox concentration. do.

For the ethanol extract and fraction of lemon myrtle of the present invention, free radical scavenging ability was confirmed using ORAC assay.

This experiment was also commissioned by the Korean Dermatological Research Institute (test number KDRI-IV-2020-076), and the results are shown in FIG. 3 .

As a result, both the ethanol extract of lemon myrtle and the solvent fraction were statistically significant ( p <0.05) in the concentration range of 2 to 50 μg/ml, and showed concentration-dependent peroxidation radical scavenging activity. As a result of converting this into Trolox equivalent ORAC value (μM TE/100g) per unit weight, the ethanol extract of lemon myrtle was able to confirm the ORAC value of 119.85 × 106 μM TE per 100g in the 20 μg/ml treatment group, n- The butanol fraction showed an ORAC value of 110.86 × 106 μM TE per 100 g in the 20 μg/ml treatment group, and the ethyl acetate fraction showed an ORAC value of 143.53 × 106 μM TE per 100 g in the 20 μg/ml treatment group.

That is, it was confirmed that both the lemon myrtle extract of the present invention and its solvent fraction had excellent antioxidant activity.

Experimental Example 3. Confirmation of tyrosinase inhibitory activity of lemon myrtle extract

Tyrosinase (Tyrosinsase, TRP1, TRP2) is a melanin formation-related enzyme, and when melanin is formed by the melanin formation-related enzyme, pigmentation occurs on the skin.

That is, since tyrosinase activity is a very important factor in the melanin synthesis process in vivo, a substance inhibiting the activity of tyrosinase can be used as a whitening agent. Representative tyrosinase activity inhibitory ingredients include Kojic acid, Arbutin, and Vitamin C compounds.

The present inventors confirmed the effect on the melanin biosynthesis process initiated by Mushroom-derived tyrosinase with respect to the ethanol extract and solvent fraction of lemon myrtle in-vitro , and confirmed the potential as a whitening functional material through this.

This experiment was also commissioned by the Korean Dermatological Research Institute (test number KDRI-IV-2020-075), and the results are shown in FIG. 4 .

As a result, as shown in FIG. 4, both the ethanol extract and the solvent fraction of lemon myrtle showed a concentration-dependent and statistically significant ( p <0.05) level of tyrosinsase inhibitory activity in the range of 6.25 to 400 μg/ml. In particular, in the case of lemon myrtle extract, 93.67% (IC ₅₀ = 133.63 μg/ml) at a concentration of 400 μg/ml, and 79.75% at a concentration of 400 μg/ml in the case of the lemon myrtle extract n-BuOH fraction (IC ₅₀ =179.82 μg/ml) , and the lemon myrtle extract ethyl acetate fraction showed 82.03% (IC ₅₀ =147.34 μg/ml) tyrosinase inhibitory activity at a concentration of 400 μg/ml of the sample, confirming the whitening effect.

That is, it was confirmed that the lemon myrtle extract and its fractions of the present invention exhibited tyrosinase inhibitory activity of 50% or more, thereby having potential as a whitening material.

Experimental Example 4. Confirmation of melanin production inhibitory activity of lemon myrtle extract

Through Experimental Example 3, it was confirmed that the lemon myrtle extract of the present invention and its fractions inhibited the activity of tyrosinase involved in melamine formation. In order to confirm the effect on actual melanin production, B16-F10 melanoma cells were used An experiment was conducted to confirm the melanin production inhibitory activity.

Specifically, B16-F10 melanoma cells were cultured in a 150mm cell culture dish using DMEM medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S) at 37°C, 5% CO ₂ Cultured in an incubator. Cells that reached quorum were subcultured using Trypsin-EDTA.

Thereafter, a concentration range that does not exhibit cytotoxicity was confirmed through a cell viability test. The ethanol extract of lemon myrtle and the n-butanol fraction showed a cell viability of 90% or more at a concentration of 50 μg/mL or less, but the ethyl acetate fraction showed a cell viability of 90% or more at a concentration of 25 μg/mL or less.

In this experiment, the lemon myrtle extract of the present invention and its fractions were diluted by concentration to a concentration that does not show cytotoxicity, and the amount of melanin production was measured.

Specifically, the experiment was conducted through the following process.

① B16-F10 cells were cultured in a DMEM medium supplemented with 10% FBS and 1% P/S at a concentration of 8×10 ⁴ cells/well in a 6-well cell culture plate for 24 hours. ② 100 μM α-Melanocyte stimulating hormone (α-MSH) was diluted in DMEM/Modified medium to prepare DMEM/Modified medium containing 100 nM α-MSH. ③ Samples and positive control, β-arbutin, were prepared by diluting each concentration in DMEM/Modified medium (100 nM α-MSH). ④ After removing the culture solution of the cells cultured for 24 hours, the cells were washed with 2 mL of DPBS. ⑤ The samples prepared by diluting each concentration were treated 3 mL each, and incubated at 37° C., 5% CO ₂ in an incubator for 48 to 72 hours. ⑥ After the culture was completed, the cells and the culture medium were recovered and centrifuged. ⑦ 100 μL of the supernatant was dispensed into 96 well plates, and the absorbance was measured at 490 nm to measure the amount of extracellular melanin release. ⑧ After removing the remaining supernatant, the cells were washed with 1 mL of DPBS, and then dried at 40°C. ⑨ After adding 120 μL of 1N NaOH (10% DMSO) to the dried cells, the cells were lysed by boiling in a water bath at 60° C. for 1 hour and centrifuged at 15000 rpm for 1 hour. ⑩ 100 μL of the supernatant was dispensed into a 96-well plate, and the absorbance was measured at 490 nm to measure the amount of melanin production in the cells. ⑪ The measured amount of melanin was corrected with the total protein amount obtained by BCA (Bicinchronic acid assay) and the synthetic melanin standard curve.

The inhibition rate of melanin production was calculated using the following formula.

[Formula 1] Melanin production inhibition rate (%) = 100 - [(Melanin production in the sample addition group / Melanin production in the α-MSH treatment control group) x 100]

This experiment was commissioned by the Korean Dermatological Research Institute (test number KDRI-IV-2020-077), and the results are shown in FIGS. 5 to 7 .

As a result, it was confirmed that the lemon myrtle ethanol extract inhibited the total intracellular and extracellular total melanin production at a statistically significant level (p<0.05) at the 50 μg/mL treatment concentration, and it was confirmed that it inhibited the total cell melanogenesis by 17.49%. .

Even in the case of the ethyl acetate fraction, it was confirmed that it inhibited the intracellular and extracellular total melanin production at a statistically significant level (p<0.05) at the 25 μg/mL treatment concentration, and it was confirmed that 14.47% of the total cell melanogenesis was inhibited.

On the other hand, in the case of the n-butanol fraction of lemon myrtle, the inhibitory effect of intracellular/extracellular total melanin production did not appear in the concentration range that did not affect the B16-F10 cell survival.

That is, it was confirmed that the ethanol extract and ethyl acetate fraction of lemon myrtle of the present invention inhibited melanin production and had a whitening effect.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

Claims (10)

A composition comprising a lemon myrtle extract or a solvent fraction thereof. According to claim 1,
The lemon myrtle extract is extracted by cold extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and extrusion extraction alone or by using two or more methods in combination. 3. The method of claim 2,
The extraction solvent used for extracting the extract is water, C1 to C4 lower alcohol, glycerin, butylene glycol, propylene glycol, methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, dichloromethane, or their A composition using a mixture. According to claim 1,
The solvent fraction is obtained by using n-butanol and ethyl acetate, the composition. 5. The method according to any one of claims 1 to 4,
The composition will exhibit antioxidant and whitening effects, the composition. 6. The method of claim 5,
The antioxidant effect is to remove active oxygen (ROS) and free radicals (free radicals), the composition. 6. The method of claim 5,
The composition will exhibit tyrosinase inhibitory activity. A composition for inhibiting active oxygen and melanin production, comprising at least one of an ethanol extract of lemon myrtle or an ethyl acetate fraction thereof. A cosmetic composition for skin anti-aging and whitening, comprising the composition of claim 5. A cosmetic composition for skin anti-aging and whitening, comprising the composition of claim 8.

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