JP2010202520A - Skin improver and oral composition for cosmetic and health purposes - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To develop a comfortable, safe, stable material recovering from reduction of extracellular matrix component content in a living body, particularly in a skin tissue, resulting from a decline in metabolic function caused by ultraviolet, active oxygen, aging, etc., so as to prevent and/or ameliorate skin troubles, and to provide a composition making efficient industrial use thereof. <P>SOLUTION: Lemon myrtle and/or an extract thereof are activating skin fibroblasts, enhancing proliferation thereof, enhancing production of extracellular components by the fibroblasts, producing moisturizing effect and markedly improving aging symptoms of the skin. Lemon myrtle and/or the extract thereof can be effectively used in areas such as food and drink, pharmaceutical products, quasi drugs and feeds. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a skin improving agent comprising lemon myrtle and / or an extract thereof as an active ingredient, and an oral composition for cosmetic health comprising the same. More specifically, the skin improving agent is a dermal fibroblast proliferation promoter, collagen and / or hyaluronic acid production enhancer, skin anti-aging agent and moisturizer.

  The skin consists of the epidermis, dermis and subcutaneous tissue. Of these, the dermal tissue is mainly occupied by extracellular components such as proteins such as collagen and mucopolysaccharides such as hyaluronic acid, etc., supporting cells and skin tissue, water retention in the interstitial space, maintaining skin lubricity and flexibility. And so on. These extracellular components are produced by fibroblasts (Non-Patent Document 1). The extracellular component produced by fibroblasts is decomposed by irradiation with active oxygen or ultraviolet rays, and induces skin troubles such as skin wrinkles, spots, and freckles. In addition, it is known that the content of extracellular components such as hyaluronic acid decreases with aging (Non-patent Document 2). When the content of hyaluronic acid or collagen in the skin tissue decreases, skin troubles such as a decrease in elasticity and flexibility, an increase in wrinkles, and skin aging symptoms are caused. Therefore, in order to maintain healthy skin, it is necessary to replenish the extracellular component. For this purpose, it is desirable to activate fibroblasts that are the component-producing cells.

  The activating substances for dermal fibroblasts have heretofore been extracts such as hibiscus extract (Patent Document 1), L-ascorbic acid and its derivatives (Patent Document 2), almond, dandelion, embroidery, hop, etc. (Patent Document 3). ), Collagen hydrolyzed tripeptide (patent document 4), rosemary extract containing genquanine (patent document 5), α-D-glucopyranosylglycerol (patent document 6), polypeptide having a specific amino acid sequence (patent document 6) Patent Document 7) and the like have been proposed. These components and extracts have been disclosed, for example, as being incorporated into cosmetics and external preparations and applied to the skin. However, they did not essentially improve the physiological function of skin tissue. In addition, when peptides are taken orally, there is a risk of deterioration and degradation in the gastrointestinal tract, and there are few things that can be effective in practical use.

  It is also possible to prevent skin troubles and skin aging by protecting the skin from physical methods, that is, drying. For this purpose, water-soluble polyhydric alcohol typified by glycerin, water-soluble polymer typified by hyaluronic acid, natural moisturizing factor typified by pyrrolidone carboxylate, intercellular lipid typified by ceramide, etc. Has been used as a humectant. Many water-soluble polyhydric alcohols and water-soluble polymers have a sticky feeling and are not preferable in terms of sensory characteristics. In addition, since natural moisturizing factors and intercellular lipids are restricted by the concentration and preparation at the time of blending skin cosmetics, the dosage form is limited, and the effect may not be sufficiently exhibited. Therefore, development of a new moisturizing agent is required. Recently, various moisturizing ingredients such as chitin / chitosans, protein hydrolysates, and acidic mucopolysaccharides such as hyaluronic acid have been studied as moisturizing ingredients with high moisture retention. However, these moisturizing ingredients have an unpleasant stickiness, and there are drawbacks such as the amount of addition being limited due to an unpleasant odor.

  By the way, Lemon Myrtle is one of the bush herbs native to Australia, and it contains a citral component rather than lemon, and its antibacterial and relaxation effects are known for its use in soaps and deodorants. However, there was no report that it was used for skin function improvement such as skin anti-aging agent, and the aromatic component that is one of its features was an oil-soluble substance, so it was mainly used as herbal oil .

Japanese Patent Laid-Open No. 9-295928 Japanese National Patent Publication No. 10-509735 Japanese Patent Laid-Open No. 10-36279 JP 2002-255847 A JP 2004-137217 A JP 2004-331578 A JP 2006-265221 A JP-A-61-37710 JP-A-10-279466

Michihiro Hattori, “Science of Skin Care”, pp. 6-14 and pp. 15-83, Hankabo Co., Ltd., published on February 25, 1997 Maria O. Longas et al., Carbohydr. Res. 159, 127-136 (1987)

  In order to recover the reduction of the extracellular matrix component content in the living body, particularly skin tissue, caused by a decrease in metabolic function caused by ultraviolet rays, active oxygen, aging, etc., and to prevent and / or improve the skin troubles An object of the present invention is to develop a material that is comfortable, safe and stable, and to provide a composition that can be effectively used industrially.

  In order to solve the above problems, the present inventors have conducted extensive studies on the metabolic mechanism of the extracellular component in skin tissue and materials that promote its production. Lemon Myrtle activates skin fibroblasts, promotes their proliferation, enhances the production of the extracellular components by the cells, creates a moisturizing effect, and significantly improves skin aging symptoms. In addition, the present inventors have found that it can be effectively used in the fields of foods and drinks, pharmaceuticals, quasi drugs, feeds, and the like, and have completed the present invention.

That is, the present invention has been completed based on such knowledge and includes the following inventions.
(1) A skin fibroblast growth promoter comprising lemon myrtle and / or an extract thereof as an active ingredient.
(2) A collagen and / or hyaluronic acid production enhancer comprising lemon myrtle and / or an extract thereof as an active ingredient.
(3) The collagen and / or hyaluronic acid production enhancer according to claim 2, wherein the production enhancement of collagen and / or hyaluronic acid is an increase in the amount of collagen and / or hyaluronic acid in the skin tissue.
(4) A skin aging inhibitor comprising lemon myrtle and / or an extract thereof as an active ingredient.
(5) A humectant comprising lemon myrtle and / or an extract thereof as an active ingredient.
(6) An oral composition for beauty and health comprising the agent according to any one of (1) to (5).
(7) The oral composition for beauty and health according to (6), wherein the oral composition is a food or drink.

  The lemon myrtle and / or extract thereof according to the present invention is excellent in quality stability, has a moisturizing effect, promotes the proliferation of skin fibroblasts, and enhances the production of collagen and / or hyaluronic acid by the fibroblasts. In addition, it promotes skin turnover and has the effect of improving skin troubles such as skin wrinkles, spots, dullness, buckwheat, sagging, roughness, and rough skin. In addition, it promotes regeneration of damaged skin and contributes to maintaining skin health. Such an effect is remarkably expressed by orally ingesting or administering the dermal fibroblast proliferation promoter, collagen and / or hyaluronic acid production enhancer, skin aging inhibitor or moisturizer of the present invention. Therefore, the respective agents of the present invention improve the skin, particularly in the fields of foods and drinks, pharmaceuticals, quasi-drugs, feeds, etc., in the form of the respective agents or in a form blended with various conventional products in the fields. It is possible to make effective use for this. The respective agents of the present invention can also be applied to products in the fields of cosmetics and external preparations for skin.

  The present invention is described in detail below.

  Lemon myrtle used in the melanin production inhibitor of the present invention is a kind of herb and has a scientific name of Backhouse citriodora. Although not particularly limited, the extract used in the present invention mainly uses leaf portions. Lemon myrtle is generally widely used and can be easily obtained as a natural dry product.

  The extract can be produced by any method, but is preferably extracted using an extraction solvent such as water, lower alcohol, hexane or chloroform. The lower alcohol has a tendency to extract the oily component in the defatted soot as the carbon number increases, so a lower alcohol is desirable, such as methanol, ethanol, normal propanol, isopropanol, normal butanol, isobutanol, etc. It can be illustrated.

  Although there is no restriction | limiting in particular in the method at the time of manufacturing an extract, About 1 weight part or more of the said extraction solvent is added with respect to 1 weight part of lemon myrtle, under pressure of 1 atmosphere or more, or pressure reduction of 1 atmosphere or less, 30 The mixture is stirred and mixed as necessary for several minutes or more at a temperature of ℃ or higher, then cooled and filtered, and the filtrate is concentrated and dried by an appropriate means such as drying under reduced pressure, spray drying or freeze drying. The lemon myrtle may be raw or dried, and may be appropriately pulverized. In this way, the extract according to the present invention can be obtained. In the extraction method, the extraction residue once extracted can be repeatedly extracted.

  The dermal fibroblast proliferation promoter of the present invention may be used as the dermal fibroblast proliferation promoter as it is in the form of a solid, paste or liquid as the active ingredient. Accordingly, a known additive for use in which the dermal fibroblast growth promoter of the present invention is used can be used in combination with a conventional method to prepare a composition. Here, known additives are preferably those commonly used for ingestion, for example, excipients, binders, disintegrants, lubricants, wetting agents, fluidizing agents, preservatives, surfactants. , Stabilizers, diluents, solubilizers, tonicity agents, bactericides, preservatives, flavoring agents, flavoring agents, coloring agents, fragrances and the like can be used, and the effect of promoting fibroblast proliferation is known Material can be used.

  In addition to the materials described in the above-mentioned patent document, the fibroblast proliferation promoting action is known as chlorella, aloe vera, rice, jujube, moon peach, mango ginger, no grape, spinach fern, lotus germ, sesame, chili pepper, toki, Dokudami, Haskup fruit, Kusunohagashi, algae (kaurelpa, racemosa), dried products or extracts of plants and algae such as strawberry, barley, catechins, imino group-containing peptides, α-lipoic acid and its salts, esters, amides, etc. Examples thereof include derivatives, dihydrolipoic acid and derivatives thereof, chitin hydrolysates, N-acetyl-D-glucosamine and oligomers thereof. In addition, this invention is not limited at all by these illustrations.

  In addition, the form of the said composition can be made into oral preparations, such as a granule, a tablet, a capsule, and a liquid agent. The content of the extract in such a pharmaceutical composition is not particularly limited. Although it is difficult to specify uniformly depending on the type and content of the combined use raw material, it is 50% by weight or less, more desirably 10% by weight or less. The fibroblast growth promoter of the present invention is preferably used in a mode of being taken or administered orally. A suitable amount of the fibroblast growth promoter of the present invention when orally ingested or administered is 3,000 mg or less, preferably 1,000 mg or less per day for a human adult.

  Next, the collagen and / or hyaluronic acid production enhancer of the present invention has an action of enhancing the production of collagen and / or hyaluronic acid in a living body, and contains the extract as an active ingredient. It is characterized by that.

  The extract applied to the collagen and / or hyaluronic acid production enhancer of the present invention is the extraction method and conditions, blending amount, concomitant raw materials, composition and form, utilization method, intake, etc. The same as in the case of a growth promoter. In the collagen and / or hyaluronic acid production enhancer of the present invention, the production of collagen and / or hyaluronic acid is more desirably the production of collagen and / or hyaluronic acid by fibroblasts in the dermal tissue.

  Further, the skin antiaging agent and moisturizing agent of the present invention have an action of improving skin troubles and damages such as skin wrinkles, spots, buckwheat and the like, and contain the extract as an active ingredient. Features.

  The extract applied to the skin antiaging agent and moisturizing agent of the present invention is the above-mentioned skin fibroblast proliferation with respect to the extraction method and conditions, blending amount, combined raw materials, composition and form, usage method, intake amount, etc. Same as for accelerator. In the skin antiaging agent of the present invention, the skin aging symptom preferably includes at least one symptom selected from the group consisting of wrinkles, spots, dullness, buckwheat, sagging, roughness and rough skin.

  In the present invention, at least one of the above-described dermal fibroblast proliferation promoter, collagen and / or hyaluronic acid production enhancer, skin aging inhibitor and moisturizer is used as it is in the form of a food, beverage, pharmaceutical product, or pharmaceutical part. It can be made into various products in the external field, feed, and other industrial fields, or can also be used in an aspect of being used as a part of a blended raw material of the various products. In particular, an oral composition for beauty and / or health is preferred, and the most preferred embodiment of this oral composition is a food or drink. This example will be described below, but the present invention is not limited thereby.

  Specific examples of foods and drinks are not particularly limited, but vegetable juice, carbonated drinks, nutritional drinks, fruit juice drinks, soft drinks, sports drinks, beverages such as tea, soup, jelly, pudding, yogurt, cake premix products , Confectionery, sprinkles, miso, soy sauce, sauce, dressing, mayonnaise, vegetable cream, seasonings such as yakiniku sauce and noodle soup, noodles, udon, soba noodles, spaghetti, processed meat and fish products such as ham and sausage, hamburg , Croquettes, sprinkles, boiled rice cakes, jams, milk, cream, butter, spreads and cheese powders, solid or liquid dairy products, margarine, bread, cakes, cookies, chocolate, candy, gummi, gum In addition to processed foods, powder, granules, pills, tablets, soft capsules, hard capsules, paces Jo or liquid dietary supplement, food for specified health uses, functional food can include healthy foods, the concentrated liquid diet and dysphagia food diet like. Moreover, you may add the skin beautifying composition of this invention to an instant foodstuff. For example, a composition for beautifying skin that is spray-dried or freeze-dried together with powdered cellulose can be easily contained in a food or drink by making it into powder, granule, tableting or solution.

  In order to produce these foods and drinks, at least one of the dermal fibroblast growth promoter, collagen and / or hyaluronic acid production enhancer, skin aging inhibitor and moisturizer of the present invention and a known raw material are used. Alternatively, a part of a known raw material may be replaced with at least one of the above-mentioned agents and manufactured by a conventional method. For example, the dermal fibroblast proliferation promoter of the present invention and, if necessary, excipients such as glucose (dextrose), dextrin, lactose, starch or processed product thereof, cellulose powder, vitamins, minerals, animals and plants and fish and shellfish Oils and fats, proteins (proteins derived from plants and animals, hydrolysates thereof, etc.), carbohydrates, pigments, fragrances, antioxidants, surfactants, other edible additives, powders and extracts containing various nutritional functional ingredients Mixed with edible ingredients such as powders, granules, pellets, tablets, etc., processed into ordinary processed foods of the above examples by conventional methods, mixed liquids such as gelatin, sodium alginate, Using capsules such as carboxymethylcellulose to form capsules or processing into beverages (drinks) for use as dietary supplements or health foods It is suitable. In particular, tablets, capsules and drinks are desirable.

  The ratio of at least one of the dermal fibroblast proliferation promoter, collagen and / or hyaluronic acid production enhancer, skin anti-aging agent and moisturizing agent of the present invention to be blended in such food and drink is the type and form of the food and drink The content of the aqueous component according to the present invention in the agent of the present invention is difficult to define uniformly depending on the type and component of the other compounding ingredients and the blending amount, but the content of the aqueous component in the food and drink At least one of the dermal fibroblast proliferation promoter, collagen and / or hyaluronic acid production enhancer, skin aging inhibitor and moisturizer of the present invention so as to be 50% by weight or less, more desirably 10% by weight or less. The formulation may be designed by appropriately combining the agent with other known raw materials for producing food and drink, and the desired food and drink may be prepared according to conventional methods. In the case of a human adult, the food and drink according to the present invention can be used in any method, for example, at the same time or before or after the intake of a meal, with a guideline of intake of the aqueous component per day being 3,000 mg or less, preferably 1,000 mg or less. It can be taken into the body by methods such as oral intake and tube administration.

  Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited by this. In each example, all the percentages, parts and ratios are based on weight.

(Production Example 1)
60 g of dried product of lemon myrtle leaves, chopped, add 200 ml of water, heat at 70 ° C. for 1 hour, cool to room temperature, remove insolubles by filtration, concentrate under reduced pressure, and then freeze It dried and obtained about 2 g of extract (referred to as sample 1).

(Production Example 2)
120 mL of ethanol (purity 99.5%) was added to 60 g of the dried product of the leaves of the lemon myrtle, heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. 120 mL of ethanol (purity 99.5%) was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 2 g of the extract (referred to as sample 2) according to the present invention.

Example 1
The present inventors performed a fibroblast proliferation promoting evaluation test using MTT reduction method based on human-derived normal skin fibroblasts (see TIM Mosmann; Journal of Immunological Methods p55-63, 1983). .

<Test method> Using DMEM (Gibco) containing 5% FBS (fetal calf serum; purchased from Nikkyo), human-derived normal skin fibroblasts (manufactured by Kurabo Industries) are 2 × 10 ⁴ cells / well in a 96-well plate. And then cultured at 37 ° C. and 5% CO _{2 for} 24 hours. After removing the medium, the medium was washed with PBS (−) (Nissui Pharmaceutical), the sample was replaced with 1% FBS-containing DMEM containing each concentration, and cultured at 37 ° C. and 5% CO ₂ . At this time, the blank is MEM containing 1% FBS not including the test sample. After culturing for 48 hours, the amount of MTT reduction was determined by measuring absorbance at 550 nm by the MTT reduction method. The results are shown in Table 1. In addition, the cell activation rate was represented by the percentage% which set the light absorbency of the cultured cell (control) without a sample to 100.

  (Table 1) Cell activation activity against fibroblasts by MTT reduction method

  As is clear from Table 1, it was considered that the addition of the test substance had a higher cell activation rate than that without the test substance, and thus activated human skin-derived normal fibroblasts.

(Preparation Example 1)
Table 2 shows the skin lotion formulation as Preparation Example 1. In Table 2, the components (1) to (7) were mixed and dissolved in (9) to be uniform, and after adding (8) and mixing, the total amount was 100 wt% in (9).
(Table 2) Skin lotion formulation containing lemon myrtle extract

(Preparation Example 2)
Table 3 shows the formulation of the skin emulsion as Preparation Example 2. In Table 3, the oil phase components (1) to (5) were mixed, dissolved and made uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (6), (7), (9), (10), and (13) were mixed, dissolved, and heated to 75 ° C. Next, the oil phase component was added to the aqueous phase component and pre-emulsified, and (8) was added thereto, followed by uniform emulsification with a homomixer. After cooling, (10) was added to adjust the pH, and (12) was added and mixed at 50 ° C.

  (Table 3) Lemon Myrtle Extract-containing skin emulsion formulation

(Preparation Example 3)
As Preparation Example 3, the skin cream formulation is shown in Table 4. In Table 4, the oil phase components (1) to (7) were mixed, dissolved and made uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (8), (9) and (10) were mixed and dissolved, and heated to 75 ° C. Next, the oil phase component was added to the aqueous phase component and pre-emulsified, and then uniformly emulsified with a homomixer. Thereafter, the mixture was cooled, and (11) was added and mixed at 50 ° C.
(Table 4) Lemon Myrtle Extract-containing skin cream formulation

(Preparation Example 4)
Table 5 shows the formulation of the O / W type emulsion ointment type external skin preparation as Preparation Example 4. In Table 5, the oil phase components (1) to (5) were mixed and dissolved to be uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (5), (6), (7) and (10) were mixed, dissolved and heated to 75 ° C. Subsequently, the oil phase component was added to the water phase component to emulsify, and after cooling, (8) and (9) were added and mixed at 50 ° C.

  (Table 5) O / W type skin ointment formulation containing lemon myrtle extract

(Preparation Example 5)
160 parts of sample 1, 30 parts of beeswax and 60 parts of neutral fatty acid triglyceride (Nisshin Oillio Co., Ltd.) were heated and mixed at about 50 ° C. to homogeneity, and then subjected to a capsule filling machine. A gelatin-coated soft capsule preparation having an internal volume of 250 mg was produced. This capsule preparation can be used as a dietary supplement that can be taken orally.

(Preparation Example 6)
50 parts of sample 1, 20 parts of α-lipoic acid (manufactured by Degussa, Germany, trade name: ALIPURE (registered trademark)), 20 parts of lotus germ extract powder (manufactured by Maruzen Pharmaceutical Co., Ltd.), creatine (Degussa, Germany) , "Crea Pure") 25 parts, Riboflavin (DSM Nutrition Japan Co., Ltd.) 18 parts, Maltitol (Towa Kasei Co., Ltd.) 92 parts, Tricalcium phosphate (Yoneyama Chemical Co., Ltd.) 100 parts and 25 parts of cellulose were charged into a mixer and stirred and mixed for 10 minutes. This mixture was subjected to a direct compression tableting machine to prepare uncoated tablets having a diameter of 7 mm, a height of 4 mm, and a weight of 150 mg / piece, and then a shellac film was formed by the coating machine to produce a tablet-shaped food.

(Preparation Example 7)
0.1 g of sample 1 according to the present invention was added to 100 mL of commercially available grape juice and mixed well to produce a homogeneous grape flavored drink. Even when stored in the refrigerator for 3 weeks, the appearance and flavor were neither abnormal nor uncomfortable.

Claims (7)

A skin fibroblast growth promoter comprising lemon myrtle and / or an extract thereof as an active ingredient. A collagen and / or hyaluronic acid production enhancer comprising lemon myrtle and / or an extract thereof as an active ingredient. The collagen and / or hyaluronic acid production enhancer according to claim 2, wherein the production enhancement of collagen and / or hyaluronic acid is an increase in the amount of collagen and / or hyaluronic acid in the skin tissue. A skin aging inhibitor comprising lemon myrtle and / or an extract thereof as an active ingredient. A humectant comprising lemon myrtle and / or an extract thereof as an active ingredient. An oral composition for beauty and health comprising the agent according to any one of claims 1 to 5. The oral composition for beauty and health according to claim 6, wherein the oral composition is a food or drink.