KR20140027947A - Beautiful-skin-promoting agent and use thereof - Google Patents

Abstract

The present invention provides a skin beauty accelerator for improving skin conditions such as drying of the skin, decreased elasticity, rough skin, and / or promoting skin beauty, an oral composition for promoting skin beauty by combining the skin beauty promoter, and An object of the present invention is to provide a cosmetic method for improving skin condition and / or promoting skin cosmetology. Skin comprising a combination comprising one or two or more selected from the group consisting of collagen peptides and blood flow improving agents, particularly plant extracts containing camellia seed extract, thioctic acid, resveratrol and resveratrol, as active ingredients An oral composition comprising a beauty accelerator, the skin beauty promoter, and a method for orally ingesting the skin beauty promoter or the composition are provided.

Description

Beauty promoter and its use {BEAUTIFUL-SKIN-PROMOTING AGENT AND USE THEREOF}

The present invention comprises a collagen peptide and a blood flow improving agent as an active ingredient, wherein the skin beauty promoter for improving skin troubles such as wrinkles, moisture, wrinkles, sagging, and / or making more beautiful skin, The oral composition which mix | blends a skin beauty promoter, and its use method are related.

The skin consists of the epidermis, dermis and subcutaneous tissue. The epidermis is composed of the stratum corneum, granule layer, polar layer and basal layer, and contains many cells such as keratinocytes and melanocytes, and acts to prevent invasion of harmful substances and pathogens from the outside world and to inhibit the evaporation of water. have. In the dermis, unlike the epidermis, the cells are small and are filled with extracellular components such as proteins such as collagen and elastin produced by fibroblasts, and mucopolysaccharides such as hyaluronic acid and chondroitin sulfate, and form matrix structures to form cells and skin tissues. It plays a role in protecting skin tissue from external factors such as support, maintenance of moisture in cell gaps, maintenance of lubricity and flexibility of skin, ultraviolet rays, dry environment, mechanical irritation and damage, and microbial infection. One).

By the way, the extracellular matrix component is denatured and decomposed under the influence of, for example, ordinary ultraviolet rays, and the amount of its synthesis decreases due to the breakdown of fibroblasts. By decomposing and degrading the amount of the extracellular matrix, the skin causes various skin problems such as dryness, roughness of the skin, reduction of elasticity and flexibility, reduction of wrinkles and gloss, wrinkles, sagging, and blemishes. Known. Therefore, in order to improve skin condition and / or maintain and enhance skin beauty, it is considered that it is desirable to activate the cells of the skin, and to supplement and further enhance the components reduced by, for example, ultraviolet rays. Collagen in particular accounts for about 70% per dry weight of skin tissue and is deeply involved in the viscoelasticity of the skin. In addition, since hyaluronic acid is a substance having a great influence on the moisturization of skin tissue, it is important to maintain and enhance collagen and hyaluronic acid in the skin tissue.

In order to enhance components in skin tissues including collagen and hyaluronic acid or to inhibit aging of the skin, various components have been studied conventionally, and until now, a degradation product of gelatin and / or collagen (Patent Document 1) and N-acetylglucosamine (patent Document 2), sphingomyelin (patent document 3), genkwanin (patent document 4), wild strawberry (patent document 5), and the like have been proposed.

However, in order to express the physiological effects of these materials in vivo, even if ingested in a large amount or ingested for a long time, the effect is not clear or only a certain effect can be obtained depending on the individual. Very little can be expressed. Accordingly, there has been a demand for the development of effective materials and compositions for promoting the skin care.

By the way, the blood flow improving agent is to improve the function of transporting blood to each tissue of the living body. The blood plays a very important role in supplying oxygen, nutrients, water, hormones, and the like to peripheral tissues, transporting immune cells, discharging waste products, controlling body temperature, and the like, and maintaining the function of biological tissues. By improving blood flow, it is known to alleviate symptoms such as atherosclerosis, hypertension, lowering of immunity, alopecia, fatigue, swelling, stiff shoulders, coldness, numbness of the hands and feet, blemishes under the eyes and skin blemishes.

Examples of substances that improve blood flow include, for example, ginkgo leaf extract, safflower extract, placenta extract, pepper extract, capsaicin, carrot extract, bitter extract, fucoidan, vitamin E and its derivatives (such as tocopherol acetate), pantothenic acid and salts thereof (calcium salts). , Sodium salts, and the like), glycyrrhizinic acid and glycyrrhetinic acid and salts thereof (sodium salts, potassium salts, and the like), tahibo extract, arginine and its derivatives (arginine glutamate, etc.), nicotinic acid and its derivatives (such as methyl esters), and rosemary extract , Ginger extract, gingerol, ginger roll, ginger, isoflavone, vitamin B ₃ , turmeric extract, extracts such as grape seeds, leaves and stems, rutin, rice germ fermentation extract, donkey extract, garlic extract, vinegar extract Is proposed. However, the report which examined the influence of the combination of these blood flow improving substances and the said skin antiaging component on skin tissue or a skin condition is not seen.

Japanese Patent Laid-Open No. 2004-123637 Japanese Patent Laid-Open No. 2001-48789 Japanese Patent Laid-Open No. 2005-281257 Japanese Patent Laid-Open No. 2004-137217 Japanese Patent Laid-Open No. 2003-137801

Hattori Michiko, `` The Science of Skin Care, '' pages 6 to 14, Shokabo Co., Ltd., issued February 25, 1997

In view of the present situation, the present inventors have developed a skin beauty accelerator which improves skin conditions such as drying of the skin, decreased elasticity, roughness of the skin, and / or promotes skin beauty, and blends the skin beauty promoter An object of the present invention is to provide an oral composition for promoting and a method for improving skin condition and / or promoting skin cosmetology.

MEANS TO SOLVE THE PROBLEM In order to solve the said subject, the present inventors earnestly examined the relationship between various materials and skin beauty, and as a result, the combination of a collagen peptide and the thing which has a blood flow improving effect has an unexpectedly significant effect, and has a blood flow improving effect. Among the substances, it was found to be particularly effective to use at least one selected from plant extracts containing camellia seed extract, thioctic acid, resveratrol and resveratrol with collagen peptides. Moreover, it discovered that this can be used effectively for oral compositions, such as food-drinks, feedstuffs, medicines, and quasi-drugs, and came to complete this invention.

That is, the feature of the present invention lies in a skin beauty accelerator comprising a collagen peptide and a blood flow improving agent as an active ingredient. In this skin care promoter, the collagen peptide is preferably one or two or more selected from the group consisting of collagen, gelatin and hydrolyzates thereof, and the hydrolyzate has a molecular weight (average molecular weight. Preferably from about 10,000. In addition, the blood flow improving agent is particularly preferably one or two or more selected from the group consisting of plant extracts containing Camellia seed extract, thioctic acid, resveratrol and resveratrol.

The camellia seed extract includes an aqueous component obtained by extracting and treating a defatted meal of camellia seed with water and / or a lower alcohol, and preferably includes saponins, and the saponins are particularly camellia. It is more preferable that it is 1 type, or 2 or more types chosen from a saponin A1, a camellia saponin A2, a camellia saponin B1, a camellia saponin B2, a camellia saponin C1, and a camellia saponin C2.

In addition, the thioctic acid is one or two or more selected from the group consisting of thioctic acid (including racemates), its reduced bodies, their salts, their esters, their amides and their cyclodextrin inclusions or lipid coatings. Is preferred.

In addition, the resveratrol preferably contains one or two or more selected from the group consisting of monomers of resveratrol or polymers such as ε-viniferin, and isomers and / or glycosides thereof, and the form thereof is grape, Or the extract of the legume plant is more preferable.

Another feature of the present invention is an oral composition for orally ingesting or administering the skin beauty promoter, and the oral composition is preferably food or drink. Another feature is a method for promoting skin aesthetics and / or skin care, in particular a cosmetic method, such as drying, decreasing elasticity and roughening of the skin orally ingesting collagen peptides and blood flow improving agents.

The skin care promoter of the present invention contains a collagen peptide and a blood flow improving agent, and is excellent in stability such as quality, acts on skin cells to significantly increase its proliferation, and promotes the production of skin components such as collagen and hyaluronic acid. It restores, maintains, and enhances the components in skin tissue. Among the blood flow improving agents, this action is enhanced by using at least one kind selected from camellia seed extract, thioctic acid and resveratrol with collagen peptide. This prevents and / or improves skin troubles such as drying, roughness, deterioration of elasticity and flexibility, reduction of wrinkles and gloss, wrinkles, sagging, and blemishes, and further promoting skin beauty. Exert. This effect is markedly manifested by oral ingestion or administration of the skin care promoter. For this reason, the said skin beauty promoter can be used effectively in the form of said agent or mix | blended with various conventional products, especially in the field of food-drinks, feed, medicine, quasi drug, etc. In addition, the present invention provides a cosmetic method for improving skin conditions such as dryness, decreased elasticity, rough skin of the skin orally ingesting the collagen peptide and blood flow improving agent.

Hereinafter, the present invention will be described in detail. First, the skin care promoter of the present invention is characterized by comprising a collagen peptide and a blood flow improving agent as an active ingredient.

The collagen peptides used in the skin care promoter of the present invention are oxalic leathers, bones, cartilage or tendons, such as cattle, pigs, chickens, turkeys and ostrichs, thread-tail domes, salmon, sharks, cod, tilapia, nile perch, carp, It is a peptide obtained by hydrolyzing the skin of fish such as volnac, tuna, catfish, eel, etc. as a raw material, collagen obtained by a conventional method or gelatin obtained by heat-modifying the collagen with acid, alkali or enzyme. . Here, the collagen peptide in this invention contains a collagen peptide and the extract containing this, These can be used arbitrarily. The collagen peptide is easy to use a commercial item.

In the present invention, it is preferable to use a collagen peptide obtained by hydrolyzing collagen or gelatin, and the molecular weight thereof is about 200 to about 10,000, more preferably about 200 to about 5,000, even more preferably about 200 to about 1,000 will be.

In addition, the blood flow improving agent according to the skin care promoter of the present invention includes, for example, an extract of a camellia seed, a thioctic acid, a resveratrol, a plant extract containing resveratrol, a ginkgo leaf extract, a safflower extract, a placental extract, a red pepper Extracts, capsaicin, carrot extract, bitter extract, fucoidan, vitamin E and its derivatives (such as tocopherol acetate), pantothenic acid and salts thereof (calcium salts, sodium salts, etc.), glycyrrhizin or glycyrrhetinic acid and salts thereof (sodium salts, Potassium salts, etc.), tahibo extract, arginine and its derivatives (arginine glutamate, etc.), nicotinic acid and its derivatives (methyl esters, etc.), rosemary extract, ginger extract, gingerol, gingerol, ginger, isoflavones, vitamin B ₃ , turmeric Extract, extracts such as grape seeds, leaves, and stems, rutin, rice germ fermentation extract, Angelica extract, garlic extract, and vinegar extract Although not limited to these examples, 1 type, or 2 or more types of those which have a blood flow improving effect can be used.

In this regard, the present inventors have studied blood flow improvers that can exert a more powerful skin beauty promoting effect by using in combination with collagen peptides, and as a result, extracts of the camellia seeds, thioctal acids and resveratrol or plants containing them The extract was found to be very effective. That is, the preferred skin care promoter of the present invention is one or two selected from the group consisting of the above-described collagen peptide and a plant extract containing a camellia seed extract, thioctic acid, resveratrol and resveratrol having a blood flow improving action. It is characterized by containing more than 1 type as an active ingredient.

Camellia seed extract according to the present invention will be described in detail below. Camellia refers to Camellia japonica, which belongs to the Camellia section of Theaceae Camellia, and includes, for example, wild camellias (C. japonica var. Japonica) and Yuki camellia (C. japonica). subsp. rusticana, C. japonica var. macrocarpa, C. japonica subsp. hozanensis, C. hongkongenesis, C. reticulata, Salmon camellia (C. saluenensis), C. pitardii var. Pitardii and Yunnan species (C. pitardii var. Yunnanica), C. nitidissima, Shandong camellia (same as wild camellia) , Wild flowers (same as wild camellia) and Yakushima camellia (same as apple camellia). These camellia trees may be grown or grown on the Japanese archipelago, the Korean peninsula, and Shandong peninsula, China.

In order to prepare the Camellia seed extract according to the present invention, the fruit and / or seeds of the Camellia japonica are compressed, and a supercritical extraction treatment using a hydrophobic organic solvent such as hexane or heptane or a liquefied gas such as liquefied carbon dioxide or liquefied propane. It is preferable to use, as a raw material, a degreasing material (hereinafter sometimes referred to as a degreasing foil) which is a residue obtained by providing an oil or the like and extracting an oil component by a conventional method. Here, the fruits and / or seeds of the camellia may be any of ripe and mature fruits, and may be used as their seeds. However, when the mature fruits or the seeds are used, the amount of skim or / and the active ingredient is large. It is preferable to lose. More preferably, seeds are used. As a preferable form, what dried the seed obtained from mature fruit in sunlight etc. for about 1 to 2 weeks is used.

The active ingredient of the Camellia seed extract according to the present invention is preferably an aqueous ingredient. Although this aqueous component can be manufactured by arbitrary methods using the said skim foil as a raw material, it is preferable to extract-process using water and / or a lower alcohol. Since the lower alcohol has a tendency to extract oily substance in skim foil as the carbon number becomes large, it is preferable that carbon number is up to about 5, and methanol, ethanol, normal propanol, isopropanol, normal butanol, isobutanol, etc. can be illustrated. . When using a lower alcohol having a large carbon number, it is preferable to increase the water content in order to suppress extraction of the oily component in the skim foil. For example, the water content in the case of propanol is about 20 to about 50 mass%, and the water content in the case of butanol is about 40 to about 70 mass%. Preferred extraction solvents are water, methanol and ethanol and their hydrous alcohols, more preferably water or hydrous methanol or hydrous ethanol having a water content of 50% by mass or more, still more preferably water.

In order to extract skim foil, about 1 to about 30 mass times of the said extraction solvent is added with respect to 1 mass part of skim foil, and it is about 10 minutes at normal temperature-about 120 degreeC under normal pressure or about 1 to about 5 atmospheres of pressurization. After stirring and mixing for about 3 hours if necessary, the mixture is cooled to room temperature and filtered, and the filtrate is concentrated and dried by suitable means such as drying under reduced pressure, spray drying, and freeze drying. You may grind this dried material suitably. In this way, a pale yellow or yellow red solid, which is a water-soluble component contained in the camellia seed according to the present invention, can be obtained. The extraction method is also possible to increase the amount of water-soluble components according to the present invention by carrying out the extraction residue once subjected to the extraction extraction in the same manner or by repeating the extraction at about 100 to about 130 ℃ under pressure of about 1 to about 3 atmospheres, The extract may also be provided to known means, such as fractionation by a column filled with adsorbents such as solvent fractionation, ion exchange resins, silica gel, activated alumina, and fractionation by liquid chromatography, to concentrate and purify the active ingredient. have. This water-soluble component includes saponins, tannins, and the like.

3β- [2-O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinofyranosyl) included as the saponin contained in the water-soluble component. Camelliasaponin A1 which is -β-D-glucopyranuronosyloxy] oleana-12-ene-16α, 22α, 28-triol22-[(Z) -2-methyl-2-butenoate] , 3β- [2-O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyra Camelliasaponin A2, 3β- [2-O, which is noronosyloxy) oleana-12-ene-16α, 22α, 28-triol22-[(E) -2-methyl-2-butenoate] -β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy) -16α, Camelliasaponin B1, 3β-[[2-O-, 28-dihydroxy-22α-[[(Z) -2-methyl-2-butenyl] oxy] oleana-12-ene-23-al β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinofyranosyl) -β-D-glucopy Ranulinosyl) oxy] -16α, 28-dihydroxy-22α-[[((E) -2-methyl-2-butenyl] oxy] oleana-12-ene-23-al, Camelliasaponin B2 , 3β- [2-O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyra Camelliasaponin C1 and 3β- [2 which are noronosyloxy] oleana-12-ene-16α, 22α, 23,28-tetraol22-[(Z) -2-methyl-2-butenoate] -O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy] ole Camelliasaponin C2 which is anana-12-ene-16α, 22α, 23,28-tetraol22-[(E) -2-methyl-2-butenoate], etc. can be illustrated. These saponins are specifically contained in Camellia.

In addition, the origin and type of the thioctic acid according to the present invention are not particularly limited, and it is well known such as extracts of natural products of organs such as soy sauce of cows and pigs, and chemical synthetic products using, for example, ethylene and adipic acid as starting materials. It may be collected and manufactured by the conventional method. In addition, since thioctic acid has an asymmetric carbon, enantiomers ((R) -enantiomer and (S) -enantiomer) having different optical properties exist, and the thioctic acid according to the present invention may be any of these alone. The mixture of ratios may be sufficient, and a racemate (racemic mixture and a racemic compound) ((R), (S)-thioctic acid) may be sufficient. In carrying out the industrial production level, it is easy to use a commercially available racemate which is inexpensive and easily available. Use of the racemate is preferable because it tends to express more strongly the desired effect of the present invention.

The thioctic acid used for the skin care promoter of the present invention can be suitably used in addition to the above-described thioctic acid, and is composed of thioctic acid, its reducing agent, their salts, their esters, their amides and their cyclodextrin inclusions. It is preferable that it is 1 type, or 2 or more types selected from the group.

As a specific example of the reducing agent of thioctic acid, dihydrothioctic acid, dihydrolipoic acid, 6,8- dimercapto-octanoic acid, (R), (S) -dihydrothioctic acid, etc. are mentioned.

As a salt, it is (R)-thioctic acid, (S)-thioctic acid, (R), (S)-thioctic acid, (R)-dihydrothioctic acid, (S)-dihydrothioctic acid, (R), ( Potassium salts, such as S) -dihydrothioctic acid, a sodium salt, a calcium salt, a magnesium salt, etc. are mentioned.

Examples of the ester include (R) -thioctic acid, (S) -thioctic acid, (R), (S) -thioctic acid, (R) -dihydrothioctic acid, (S) -dihydrothiocic acid, (R), ( S)-partial esters or complete esters or glycerides (monoglycers) with dihydrothioctic acid and polyhydric alcohols (monomers or polymers such as ethylene glycol, propylene glycol, butanediol, neopentyl glycol, glycerin, erythritol, polyglycerol) Lides, diglycerides, triglycerides) or mono alcohols having 10 to 22 carbon atoms (decanol, lauryl alcohol, myristyl alcohol, cetanol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, etc.) Ester etc. are mentioned.

Examples of the amides include (R) -thioctic acid, (S) -thioctic acid, (R), (S) -thioctic acid, (R) -dihydrothioctic acid, (S) -dihydrothioctic acid, (R), ( Amides, such as S) -dihydrothioctic acid, can be illustrated.

Examples of cyclodextrin inclusions include inclusions of α-, β-, γ-, or δ-cyclodextrin and the thioctic acid or derivatives thereof.

In addition, this invention is not limited by these illustrations.

In the present invention, one or two or more crystals, powders and / or particles selected from the group consisting of the above-described thioctic acid, its reducing agent, their salts, their esters, their amides and their cyclodextrin inclusions as thioctal acids The outer surface is covered with lipids, and this form is more practically preferable in order to suppress thermal deterioration (decomposition, polymerization, discoloration, etc.), moisture absorption, or oxidative degeneration of thioctic acids.

Lipids which coat the outer surface of the crystals, powders and / or particles of the thioctic acid may be acceptable in the industrial field in which the present invention is used, and common edible oil fats or industrial fats and oils, fatty acid glycerides and fatty acids , Fatty acid esters, fatty acid amides, higher alcohols, waxes, sterols, glycolipids, phospholipids and the like can be used alone or in combination. In consideration of coating workability and physical properties (stability, solidification, flowability, meltability, solubility, etc.) of these, lipids having a melting point of about 30 ° C. or more are preferable. More preferred forms are lipids having a melting point of about 40 ° C. to about 70 ° C., and still more preferred forms are lipids having a melting point of about 40 ° C. to about 60 ° C. When melting | fusing point is less than about 30 degreeC, a coating may not be able to maintain a solid state at the time of its use, may form a mass, or may impair fluidity. On the contrary, when it exceeds about 70 degreeC, there exists a possibility that the thioctic acid itself may deteriorate under the influence of heat processing and mechanical energy at the time of manufacturing a deliquescent agent or a composition which concerns on this invention.

Specific examples of such lipids include vegetable oils such as soybean oil, rapeseed oil, corn oil, sunflower oil, cottonseed oil, wheat germ oil, brown oil, sesame oil, olive oil, safflower oil, palm oil, palm kernel oil, palm oil, linseed oil, peanut oil, tallow, lard. Animal fats and oils such as fish oil, processed fats and oils subjected to one or more of such treatment as fractionation, transesterification, decolorization, deodorization, various hydrogenated oils partially or completely hydrogenated, saturated fatty acids having 2 to 22 carbon atoms (acetic acid, Butyric acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, pentadecanoic acid, palmitic acid, stearic acid, 12-hydroxystearic acid, isostearic acid, arachidic acid, behenic acid, etc. ) Or unsaturated fatty acids (palmitoleic acid, oleic acid, elideic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, ricinolic acid, arachidonic acid, icosapentaenoic acid (EPA), erucic acid, docosahexaenoic acid ( DHA), etc.), Salts of fatty acids (sodium salt, potassium salt, calcium salt, magnesium salt, etc.), esters with monohydric alcohols (methanol, ethanol, propanol, butanol, etc.), polyhydric alcohols (ethylene glycol, propylene glycol, butanediol, neopentyl Partial or complete esters with monomers or polymers such as glycol, glycerin, erythritol, or glycerides (monoglycerides, diglycerides, triglycerides), higher alcohols having 10 to 22 carbon atoms (decanol, la) Plant-derived waxes such as uril alcohol, myristyl alcohol, cetanol, stearyl alcohol, isostearyl alcohol, behenyl alcohol, etc., waxes (carnauba wax, rice wax (rice bran), candelilla wax, etc. Animal-derived waxes such as sperm, shellac wax, petroleum-derived waxes such as paraffin wax, microcrystalline wax, mineral-derived waxes such as montan wax, ozokerite, poly Styrene wax, synthetic waxes such as esters of the fatty acids and higher alcohols), sterols (eg, animal cholesterol, phytocamphor, stigmasterol, cytosterol, ergosterol derived from fungi, derivatives thereof), phospholipids (animals and animals) Derived lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidyl inositol, phosphatidylserine, phosphatidic acid, sphingomyelin, and the like Lactosyl monoglycerides, monoglucosyl diglycerides, digalactosyl diglycerides, sucrose fatty acid esters, and the like. In addition, this invention is not limited at all by these illustrations.

In the present invention, any one kind or a mixture of two or more kinds of the above-mentioned various lipids can be used, but the preferred kinds of lipids are the above-mentioned cooking oil fats or industrial fats and oils, fatty acid glycerides, fatty acid esters and waxes, and more preferably. Preferably, they are cooking oil fats and fatty acid glycerides, and one or two or more combinations thereof selected from fatty acids, higher alcohols, sterols, glycolipids, or phospholipids may be used to adjust melting point of coating lipids, strengthen membrane coating, and the like. It is a more preferable form from the point of.

Known methods can be used to coat the outer surface of crystals, powders and / or particles of thioctic acids with lipids. Namely, crystallization of thioctic acid using a ball mill, a flash blender (powder mixer), a V-type mixer, a high speed mixer, a high speed paddle mixer, a hot melt mixer, an ultrasonic humidifying liquid mixer, a tumbler mixer, a pressure extruder, and the like, A method of uniformly mixing powders and / or particles with heat-melted lipids, cooling and solidifying them, and then pulverizing them, spraying or dropping the liquid-liquefied lipids by appropriately heating the above-described form of thioctic acid, A mixture of thioctic acid and particulate lipids of the above form by high-speed agitation and contacting or colliding the same to uniformly attach and coat particulate lipids over the entire surface of crystals, powders and / or particles of the thioctic acid. Method and the like are possible. In the present invention, among these, crystals, powders and / or particles of thioctic acids and particulate lipids having a specific melting point or higher mentioned above are mixed and mixed at high speed, and both of them are contacted or collided to form particulates on the entire surface of the thioctic acid of the above-described type. It is preferable to coat the lipids uniformly.

In the coating treatment described above, the ratio of crystals, powders and / or particles to lipids of the thioctic acid is determined by the crystals, the shape and size of the powders and particles, the type and melting point of the lipids, and the thickness and properties of the coating film. Although it is difficult to define uniformly by such factors, it is about 0.05 to about 10 parts by mass of lipids, preferably about 0.1 to about 5 parts by mass, based on 1 part by mass of crystals, powders and / or particles of roughly thioctic acids. . If the lipids are less than about 0.05 parts by mass, the coating state is insufficient, making it difficult to express a desired effect. On the contrary, if the amount of the lipids exceeds about 10 parts by mass, the content of the titanic acid in the coating is low, and when the coating is used, the blending ratio is limited and practical. There is a case of losing value.

In addition, the coating material by the lipids of the thioctic acid mentioned above is more preferable in the form which coat | covers the outer surface with a hydrophilic substance, whether or not this is applied to aqueous compositions, such as a beverage, and is more preferable. . Here, the hydrophilic substance means that the outer surface of the coating by the lipids is further coated, and that the hydrophilic substance has a coating film forming ability having affinity with the aqueous substance, and as a specific example, polysaccharides (xanthan gum, guar gum, tamarind seed gum) And silium seed gums), starch and chemical starch, yeast cell wall components, glucan, mannan, sherak, soda alginate, gelatin, carrageenan, pullulan, carboxymethylcellulose, soy protein, whey protein, zein and the like. More preferably one or two or more selected from the group consisting of polysaccharides, starches, yeast cell wall components, sherak, gelatin, soy protein, zein and mannan, and more preferably a group consisting of yeast cell wall components, chelac and gelatin It is 1 type or 2 or more types chosen from.

In order to coat | cover with such a hydrophilic substance, what is necessary is just to adopt the method according to the coating method of the said lipids. That is, the hydrophilic material is suitably dissolved in water, ethanol, and other solvents, and is attached to the outer surface of thioctic acid, which is previously coated with lipids, and dried to form a coating film of hydrophilic material. Can be. Such a coating becomes a double-coated structure having a hydrophilic substance as the outermost layer, and when used in foods, feeds, cosmetics, medicines, etc., the affinity with aqueous raw materials and components is high, and these are low in water solubility. It becomes easy to produce a homogeneous composition with acids.

In the coating by the lipids of the thioctic acids as described above and the double coating in which the coating is further coated with a hydrophilic substance, these include Garcinia cambogia rind, Akashoma rhizome, Guava leaf and extracts thereof (water and / or One or two or more selected from the group consisting of extraction extracts with hydrophilic organic solvents (lower monohydric alcohols such as ethanol, acetone, etc.), fractions thereof, solvent fractions or purified products), and carnitine, more preferably Since coexistence of the akasoma rhizome extract and carnitine, and most preferably carnitine in combination, it is possible to further suppress thermal and / or oxidative denaturation and deterioration of thioctic acids, thereby obtaining a thioctic acid-containing coating having excellent stability. Thioctic acids of this type are more preferred in the present invention.

The form which contains these combined raw materials in the above-mentioned thioctic acid containing coating material (i) mixes the said combined raw material with the coating which coat | covered lipids to the crystal | crystallization, powder, and / or particle | grains of thioctic acid (ii) tea (I) a lipid in which a part of the combined raw material is dispersed or dissolved in crystals, powder and / or particles of thioctic acid, which coat lipids with a mixture of crystals, powders and / or particles of oct acids and the above-mentioned raw materials. (I) attaching and drying a solution, a dispersion or an emulsion containing the concomitant raw material and the hydrophilic substance to a coating of lipids to crystals, powders and / or particles of thioctic acid, which is coated with It is possible to do both, and it does not interfere even if it combines these forms. In the present invention, the forms of (i) and (iii) exhibit the desired effects of the present invention, are easy to manufacture, and the handling workability of the coating is also good, but the forms of (iii) and (iii) provide the desired effect more strongly. Easy to express

In such a form, the mixing ratio of the coating material in which crystals, powders and / or particles of thioctic acid is coated with lipids or lipids and hydrophilic substances and the combination raw material is about 1 part by mass of the coating material. 0.01 to about 10 parts by mass, more preferably about 0.1 to about 1 part by mass. In the case of less than about 0.01 parts by mass, the desired improvement of the effect by mixing of the combined raw materials is not recognized, and in an amount exceeding about 10 parts by mass, the thioctic acid content in the above-mentioned coating and further in the composition using the same decreases, and furthermore, To limit the content of the thioctic acid in the various products incorporating the thioctic acid-containing composition, the desired effect of the thioctic acid itself in the product step can not be expected.

The origin or type of resveratrol according to the present invention is not particularly limited, and may be one obtained and manufactured using known organic chemical methods or microorganisms or yeasts, and the skins of plants such as grapes, perillas and legumes, It is preferable that it is obtained by extracting from site | parts, such as a stem, a leaf, a vine, a sprout, a seed, a flower, and a fruit.

Plants more preferred as the origin of the resveratrol according to the present invention are grapes and grape plants, the variety of which is not particularly limited, but for example, irene, aligote, valdigui, bionier, velshlithling, ortega, Cabernet Sauvignon, Cabernet Franc, Gamer, Carignan, Garganega, Carmine, Sinomabro, Grüner Beltliner, Gebürztraminer, Kerner, Gauche, Colombobad, Musso, Sagrandino, San Giuseppe, Saint Laurent , Shasla, Chardonnay, Schnean Blanc, Shorebe, Semiillon, Sauvignon Blanc, Tanat, Zweigelt, Tempranillo, Traminer, Dolceto, Donfelder, Pino Mönier, Fulsar, Purmint, Portugieser, Mus Cat, Malvasia, Malbec, Muscatel, Müller Thurgau, Murbedre, Melon, Morio Muscato, Muscadine, Amur Grape, Aquiquine, Vidal Blanc, Geobong, Seto Giants, Sable Blanc, Delaware, Na Niagara can be cited Neo Muscat, Bacau Noir, Blanc Bacau, Kuopio Yes, Muscat Bailey, Fuji Minori, ruby romance, Kaiji, Vail sansya ku, etc. These may be appropriately used those that are native or cultivated in Japan, Chile, Italy, France and the like, and are preferably extracted from each part of the above-mentioned varieties, more preferably stems, vines, buds or flowers.

Resveratrol according to the present invention can be produced by any method, using the stems, vines, shoots or flowers of the above-described grapes themselves or those dried, shredded, ground, and soaked in a solvent for a certain period of time, Or by contacting with a heated reflux extraction solvent to obtain an extract, column chromatography using an adsorbent such as silica gel, magnesium silicate, ion exchange resin, activated alumina, cellulose, activated carbon, etc. (B) Any of the purified products subjected to the purification treatment such as solvent separation and powdered thereof by a method such as freeze drying or spray drying may be used. In the case of food use, it is preferable from the point of convenience or manufacturing cost that it is made into the powder which dried the site | part of the said plant, the powder | pulverization appropriately and the three fragments or powder of the dried product extracted with water or a hydrophilic organic solvent. Moreover, when using for a pharmaceutical use, said extract, said extract, or the purified product of high purity is preferable.

Lower monohydric alcohols such as methanol, ethanol, propanol, isopropanol, polyhydric alcohols such as propylene glycol, 1,3-butanediol, glycerin, acetone, methyl ethyl ketone, ether, petroleum ether, ethyl acetate and the like as a hydrophilic organic solvent Examples of the water or mixture of In order to efficiently obtain the extract for achieving the desired effect of the present invention, it is preferable to use ethanol, acetone, ethyl acetate, and their water-containing products as the extraction solvent. The water content of the water is, for example, about 1 to about 99% by mass, more preferably about 50% by mass or less for ethanol, about 1 to about 50% by mass for acetone, and more preferably about 10 to It is about 30 mass%, and in the case of ethyl acetate, it is about 80 to about 99 mass%, More preferably, it is about 85 to about 95 mass%. Outside these ranges, the desired effect of the present invention is reduced or the yield of the extract is lowered.

Resveratrol according to the present invention is a t-resveratrol (monomer of resveratrol), or polymers such as ε-viniferin (dimer), miyabenol C (trimer), and stilbene compounds such as isomers and / or glycosides thereof. It is targeted. In the case of the extract obtained from the plant, when the stilbene-based compound is used as the main active ingredient, it contains Kelcetin, ellagitannin, ellagic acid, minerals (for example, potassium, calcium, phosphorus and magnesium) and vitamins. There is.

In the skin care promoter of the present invention, the content of the blood flow improving agent used in combination with the collagen peptide is about 0.01% to about 50% by mass of the whole agent, preferably about 0.1% to about 20% by mass, more preferably about 0.5% to about 0.5%. It is about 10 mass%. If it is less than about 0.01 mass%, the effect by combined use is small, On the contrary, even if it uses more than about 50 mass%, the further desired effect cannot be expected. In addition, the blood flow improving agent may be optionally used alone or in a plurality of the known substances or extracts described above, preferably one or more selected from plant extracts containing camellia seed extract, thioctic acid, resveratrol and resveratrol. It is two or more types, and it is more preferable to contain all these. The ratio (mass basis) when using two or more types of plant extracts containing camellia seed extract, thioctic acid, resveratrol and resveratrol is about 20/80 to 30 / 70, more preferably 60/40 to 40/60, camellia seed extract / resveratrol is 99/1 to 30/70, more preferably 80/20 to 50/50, thioctic acid / resveratrol Is 99/1 to 30/70, more preferably 80/20 to 50/50, and the Camellia seed extract / Tioctanes / Resveratrol has a ratio of each blood flow improving agent to about 1 mass of the entire skin beauty promoter. It is preferable to contain% or more, More preferably, about 10 mass% or more. In addition, what is necessary is just to set in consideration of the content of the resveratrol in the said extract in the case of the plant extract containing resveratrol.

The above-mentioned skin care promoter is composed of one or two or more combinations selected from the group consisting of collagen peptides and blood flow improving agents, especially plant extracts containing camellia seed extract, thioctic acid, resveratrol and resveratrol as active ingredients. This may be used as it is, in the form of a powder, solid, paste or liquid form containing only the active ingredient, and used for foods, medicines, quasi-drugs, feeds and the like.

The skin care promoter of the present invention can also be used as an oral composition by appropriately using a well-known additive in the above-mentioned use in which it can be used, by conventional methods. Here, the known additives may be those commonly used for oral ingestion, for example, excipients, binders, disintegrants, lubricants, wetting agents, glidants, preservatives, surfactants, stabilizers, diluents, solubilizers, isotonic agents, fungicides, Additives, such as a preservative, a copper, a dentifier, a coloring agent, a fragrance | flavor, can be used. Furthermore, it is not limited to what was described in the said prior art document, You may use together a known component and its containing material which have a skin beauty action and / or a blood flow improving effect. The skin care promoter of the present invention can also be used as part of a blended raw material for foods, pharmaceuticals, quasi-drugs, feeds, and other products in the industrial field. In particular, it is preferable to set it as a product for beauty etc.

The form in the case of using the skin care promoter of the present invention in combination with a known additive to form an oral composition can be an oral preparation of a type such as powder, granule, tablet, capsule, liquid or the like.

Although content of the said active ingredient in such an oral composition is hard to regulate uniformly according to the kind, content, etc. of the raw material to be used together, it is about 0.1-100 mass%, More preferably, it is about 10-100 mass%. When the said content is less than about 0.1 mass%, the desired effect of this invention will no longer be recognized. The skin care promoter of the present invention is used by the method of orally ingesting or administering it. In this case, the preferred intake or dosage criteria of the oral composition of the present invention is from about 100 mg to about 100,000 mg, preferably from about 500 mg per day, to a human adult (50 kg body weight) based on the active ingredient base included in the agent. About 10,000 mg, more preferably about 1,000 mg to about 5,000 mg.

The skin beauty promoter of the present invention can be used as part of the compounding raw material of known products such as food and drink, medicine, quasi-drug and feed. Examples of practical products are described below, but the present invention is not limited at all by this example.

As a specific example of food-drinks, Beverages, such as vegetable juice, fruit juice drink, a soft drink, tea; Confectionery such as cake premix products, breads, cakes, cookies, chocolates, candies, gummi jelly and gums; Seasonings such as miso, soy sauce, sauce, mayonnaise, bulgogi sauce and noodle sauce; Noodle varieties such as instant noodles, udon noodles, buckwheat and spaghetti; Meat and meat processed foods such as ham and sausages; Powdered, solid or liquid dairy products such as milk, yoghurt, cream, butter, spread or cheese; Powder form, granule form, pill form, pill form, tablet form, soft capsule form, hard food, as well as various general processed foods such as furikake, hamburger, croquette, stewed foods, jam, soup, jelly, pudding, dressing, vegetable cream and margarine Nutritional supplements in the form of capsules, pastes or liquids, specific health foods, functional foods, health foods, rich liquid foods, and therapeutic foods for swallowing disorders.

In order to manufacture these food-drinks, the skin beauty promoter of this invention and a well-known raw material may be used, or a part of well-known raw materials may be substituted by the skin beauty promoter of this invention, and may be manufactured by a conventional method. For example, according to the skin beauty promoter of the present invention, if necessary, excipients such as glucose, glucose, dextrin, lactose, starch or processed products thereof, cellulose powder, vitamins, minerals, fats and oils of animals and plants, fish and protein (derived from plants and yeasts) Protein, hydrolyzate thereof, and the like), sugars, pigments, flavorings, antioxidants, surfactants, other food additives, mixed with food materials such as powders or extracts containing various nutritional functional ingredients, It is processed into the shape of granules, pellets, tablets, or the like, or processed into the form of general processed foods by the conventional method, or the mixed powder or liquid is coated with a coating agent such as gelatin, sodium alginate, carboxymethylcellulose, and formed into capsules. Or, it is preferable to process it in the form of a drink (drinks), and to use it as a nutritional supplement or a health food. In particular, the form of a tablet, a capsule, or a drink is preferable. In addition, the content and intake amount of the skin care promoter of the present invention contained in these food and drink are almost the same as in the case of the oral composition described above.

The medicines and quasi-drugs using the skin care promoter of the present invention are appropriately added to known oral excipients and additives to the oral skin care promoter described above within the scope of the present invention, and processed by a conventional method. Formulations, such as a tablet, a capsule, a granule, powder, and a liquid, can be used. It is orally administered and applied for the prevention or treatment of dryness, decreased elasticity, rough skin, and the like. In addition, the content and intake amount of the skin care promoter of the present invention contained in these medicines and quasi-drugs are in the case of the oral composition described above.

In addition, in order to apply the skin care promoter of the present invention to animal food or animal feed, it is formulated in the same manner as in the case of the food and drink, or blended in various known feeds or drinking water, or in the form of tablets, granules, capsules with known raw materials and additives. It can be processed into a thing of the form of formulations, such as a shape. The content and intake amount of the skin care promoter of the present invention in these feeds are almost the same as in the case of the oral composition described above.

<Examples>

Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited at all by this. In each example,%, part, and a ratio are a mass reference | standard unless there is particular notice.

Preparation Example 1 (Collagen Peptide (1))

5L of water was added to 1 kg of the catfish and the skin from which the bone was removed, and the mixture was heated to 85 ° C. under normal pressure and stirred for 1 hour to extract gelatin. The gelatin solution was cooled to 50 ° C, proteolytic enzymes were added, and enzymatic reaction was carried out for 2 hours. Thereafter, the enzyme was deactivated and filtered to separate the filtrate. The filtrate was concentrated under reduced pressure, lyophilized and triturated to give 192 g of collagen peptide (Sample 1). HPLC analysis of this collagen peptide by a conventional method found that the average molecular weight was about 1,000.

Preparation Example 2 (Collagen Peptide (2))

The scales of tilapia were treated with 0.1 N hydrochloric acid, then washed with a large amount of water and dried in the sun. 8 L of water was added to 1 kg of dry scales, and the mixture was heated to 85 DEG C under normal pressure, stirred for 1 hour, and extracted with gelatin. The gelatin solution was cooled to 50 degreeC, the proteolytic enzyme was added, and the enzyme reaction was carried out for 1 hour. Thereafter, the enzyme was deactivated and filtered to separate the filtrate. The filtrate was concentrated under reduced pressure, lyophilized and triturated to give 711 g of collagen peptide (Sample 2). HPLC analysis of this collagen peptide by a conventional method found that the average molecular weight was about 5,000.

Preparation Example 3 (camellia seed extract (1))

The dried seeds of Izu Oshima wild camellia were coarsely crushed and boiled, and then compressed to obtain a compressed foil from which the compressed oil was separated. 3 L of water was added to 1 kg of this degreased substance, and it heated at 85 degreeC under normal pressure, stirred suitably for 1 hour, cooled to room temperature, filtered, and isolate | separated the filtrate. 1 L of water was further added to this filtration residue, and similarly it heated, stirred, and cooled, and filtered and collected the filtrate. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 170 g of a powdery camellia seed extract (sample 3) comprising a water-soluble component. HPLC analysis of this extract by the conventional method showed that 7.5% of camelia saponin B2, 5.8% of camelia saponin C2, and 2.4% of camphorol, one of flavonols, were included.

Preparation Example 4 (camellia seed extract (2))

2 L of hydrous ethanol (35% water content) was added to 1 kg of the degreased product obtained in the same manner as in Production Example 3, heated to reflux at 80 ° C for 1 hour, cooled to room temperature, filtered, and the filtrate was separated. 2 L of hydrous ethanol (water content 35%) was added to this filtration residue again, it heated similarly, and after cooling, it filtered and collected the filtrate. Both filtrates were combined, concentrated under reduced pressure, lyophilized and ground to give 12.1 g of a powder (sample 4) containing a water-soluble component. HPLC analysis of the powder was carried out in the same manner as in Preparation Example 3, and found to contain 8.3% of Camellia saponin B2, 5.9% of Camellia saponin C2, and 2.6% of camphorol, one of flavonols.

Preparation Example 5 Lipid Coatings of Thioctic Acid

Rapeseed seed hardened oil (Kawaken Fine Chemicals Co., Ltd. product) melting | fusing point: 330 g of crystallized titanic acid (made by Alzchem, Germany, brand name: ALIPURE (trademark), racemate) And 200 g of flakes were added, mixed well and uniformly dispersed, and then cooled and solidified at room temperature. Subsequently, the solid was pulverized with a high speed mixer and sieved with a 100 mesh (Tileer Mesh. Hereinafter identical) to obtain a thioctic acid lipid coating (Sample 5) having a particle diameter of 150 µm or less.

Preparation Example 6 (cyclodextrin inclusions of thioctic acid)

Dissolve 100 g of the thioctic acid (manufactured by Alzchem, Germany, trade name: ALIPURE (registered trademark), racemic body) of the crystal powder in 500 mL of 50% ethanol, and α-cyclodextrin (manufactured by Waka Hemi Co., Ltd., trade name: Carbamax ( 800 g of CAVAMAX (registered trademark) (R) W6) was added, and after stirring appropriately, it was concentrated and spray-dried to obtain a cyclodextrin tetrathiocate (sample 6).

Preparation Example 7 (Resveratrol Extract)

500 g of the sun dried product of the stem of grape (cabernet Sauvignon species) was extracted with reflux with 2 L of 70% ethanol solution, and the filtrate was separated by filtration. 1 L of 70% ethanol was further added to the filtrate residue to extract reflux, and the filtrate was separated. Both filtrates were combined, concentrated under reduced pressure, and dextrin was added thereto, followed by spray drying to obtain 24 g of resveratrol extract powder (sample 7). HPLC analysis of the powder by conventional methods revealed that 6.2% of trans resveratrol and 6.0% of epsilon-viniferin were included.

Test example 1 (blood flow improvement effect)

Forty subjects (24-65 years old, 20 males, 20 females) obtained consent to participate in the test described below were divided into five groups in one group, and blood flow measurement tests were performed by a double blind method. First, the subject was placed in a room of constant temperature and humidity controlled at a temperature of 24 ± 2 ° C. and a humidity of 50 ± 10%, and stably waited for 10 minutes. Thereafter, the blood flow of the back of the hand before taking the test sample was measured using a laser Doppler (manufactured by Perimed, PeriScan PIMII). Subsequently, the control group (the placebo group) was filled with dextrin in a colored hard capsule (gelatin, which is the same below) so that the subject could not discriminate by color, and in the other group, each test sample was put in the same colored hard capsule. Each of the fillings was ingested with 100 mL of water, and after 30 minutes, 60 minutes later, the blood flow of the back of the hand was measured as above. In addition, the test sample was a Camellia seed extract (Sample 3, Sample 4), commercially available thioctic acid, thioctic acid lipid coating (Sample 5), resveratrol extract (Sample 7), commercially available Ginkgo leaf extract (BH) It was set as En Co., Ltd., and carrot extract (made by Maruzen Pharmaceutical Co., Ltd.).

The results are shown in Table 1. In the table, the numerical value is a relative value when the value before ingesting the placebo and the test substance is 100, and is represented by the mean value ± standard deviation (n = 5, ANOVA analysis). From the data in Table 1, no significant changes were observed in blood flow to the back and scalp of subjects who received placebo, but sample 3 or sample 4 (camellia seed extract), thioctic acid or sample 5 (thiocate lipid coating), and sample In the case of ingesting 7 (resveratrol extract), the blood flow value of the back of the hand was significant at both the 30 minutes and 60 minutes after ingestion to the same extent as when the ginkgo biloba leaf extract or carrot extract was known. It was confirmed that the increase.

[Table 1] Effect on blood flow promotion (blood flow) on the back of the hand

Test Example 2 (skin fibroblast proliferation promoting action)

The effect on the proliferation of skin fibroblasts was examined by the following method. In other words, a petri dish (φ10 cm) was used, and normal human adult skin fibroblasts (manufactured by Kurabo Industries, NHDF (NB), hereinafter simply referred to as cells) were subjected to 10% fetal bovine serum (Daiichi Chemical Co., Ltd.). ))) 2 × 10 ⁵ was added to D-MEM medium (manufactured by Sigma Co., Ltd., low glucose), and cultured for 4 days until subconfluent (about 80% density) was obtained. Subsequently, the medium was removed, the cells were washed twice with 5 mL of PBS and further washed with 5 mL of 0.02% EDTA solution, and then the cells were collected using 5 mL of 0.25% trypsin solution (manufactured by Nakaray Tesque Co., Ltd.). Then, the supernatant was removed by centrifugation (4 ° C, 1,000 rpm, 5 minutes), and washed twice with PBS to obtain cells. These cells were subcultured by repeat culture under the above conditions.

Using a 96-hole cell culture plate (0.32 cm ² , manufactured by Asahi Techno Glass Co., Ltd.), the passaged cultured cells were cultured in low serum medium for human skin fibroblast growth (Kurabo Co., Ltd., skin fibroblast basal medium ( 106S) 1 × 10 ⁴ cells / well were seeded in 500 mL of medium to which 10 mL of low serum growth additive (LSGS) was added), and cultured for 24 hours. Subsequently, the medium was removed, and further culture was continued for 48 hours in the low serum medium for human skin fibroblast proliferation to which each sample was added so as to have a final concentration of 5, 10 or 20 μg / mL. Thereafter, 25 µL of an MTT solution (PBS dissolved in thiazolyl blue tetrazolium bromide (manufactured by Sigma, Reagent) at a concentration of 5 mg / mL) was added thereto, followed by incubation for 1 hour. After complete removal of medium by decantation, formazan solution (25% (v / v) 0.45M acetic acid buffer (pH4.5), 25% (v / v) N, N-dimethylformamide, 10% (w / v) 100 μL of sodium n-dodecyl sulfate, pH 4.5) was added and stirred. After standing at room temperature overnight, the absorbance at 590 nm was measured, and the degree of proliferation of the cells was evaluated. In the above method, D-MEM medium and low serum medium for human skin fibroblast proliferation were added with penicillin (final concentration 100 IU / mL) and streptomycin (final concentration 0.1 mg / mL). It was carried out in a CO ₂ incubator (37 ° C., 5% CO ₂ enhanced gas phase). In addition, the test sample was the collagen peptide (Sample 1, Sample 2), Camellia seed extract (Sample 3, Sample 4), commercially available thioctic acid, thioctic acid lipid coating (Sample 5), and thioctic acid cyclodextrin clathrate. (Sample 6), Resveratrol extract (Sample 7), commercially available Ginkgo biloba leaf extract (made by BH Co., Ltd.), carrot extract (made by Maruzen Pharmaceutical Co., Ltd.), and sample 1 or 2 together with each sample It was made.

The results are shown in Tables 2, 3, and 4. In the table, the numerical values are shown as relative values when the value of the control test (when no sample is added) conducted at the same time is 100. In Table 2, Samples 1 and 2 were added to the medium so that the final concentrations were 50 and 100 μg / mL, and the other samples had 5 and 10 μg / mL final concentrations. In Table 3 and Table 4, the sample 1 and the sample 2 were 50 micrograms / mL, and the other samples were added to the culture medium by 5 micrograms / mL.

From the data in Table 2, at each concentration of each sample, although the effect of proliferating skin fibroblasts was weak, it was recognized.

In addition, from the data in Table 3, when Samples 1 to 7 and thioctic acid were used in combination with Sample 1, the effect of synergistically proliferating fibroblasts became clear. Further, the proliferation effect was enhanced by using Sample 1, Sample 3, Sample 7, and Thioctic Acid in combination. However, the effect of enhancing the proliferation of fibroblasts could not be confirmed in the ginkgo leaf extract and carrot extract.

Although the effect of raising the proliferation of fibroblasts was also confirmed from the data of Table 4, it was recognized that the proliferation effect was lower than that of the data of Table 3.

In addition, as a result of similar tests using collagen peptides having an average molecular weight of about 300 instead of Sample 1 or Sample 2, the fibroblast proliferation promoting effect by the combination was equal to or more than the trend of the data in Table 3 (data omitted) ).

[Table 2] Effect of each sample on the proliferation of skin fibroblasts

TABLE 3 Effect of combination with sample 1 on proliferation of fibroblasts

TABLE 4 Effect of combination with sample 2 on proliferation of fibroblasts

From the above, it has been clarified that the combination of the collagen peptide and the blood flow improving agent according to the present invention, in particular, the Camellia extract, the thioctic acid and the resveratrol, has a function of significantly promoting the proliferation of human skin fibroblasts.

In addition, although test data is abbreviate | omitted, when the sample 1 or the sample 2 and the said three types of blood flow improving action components are used together in this test example, compared with the case where each was used alone, the collagen production ability by human skin fibroblasts, It was confirmed that the hyaluronic acid production capacity is remarkably high.

Test example 3 (skin beauty test in human)

60 adult women (35 to 55 years old, average age: 48.6 years old) of volunteers whose skin elasticity and moisture content were reduced to consent to participate in the test described below were divided into 5 groups of 1 group each Each sample was taken and continued for 6 weeks. Skin viscoelasticity measuring device (Courage + Khazaka, Germany, product name: CUTOMETER MPA580 (R)) was measured for skin elasticity before and after sample ingestion. The relative moisture of the site was measured using a moisture measuring device (Corneometer (R) CM825). In addition, this test was done by the sample 1, the sample 3, the sample 7, thioctic acid, these combinations, and the same ginkgo leaf extract which were mentioned above. The intake amount was 5 g of sample 1 and 200 mg of the other samples, and in combination with sample 1, 5 g of sample 1 and 200 mg of other samples were used together.

The results are shown in Table 5. The skin elasticity and water content before and after taking the sample showed a slight improvement after the intake of each sample other than the Ginkgo biloba extract, but there was no significant difference before and after the intake. However, in the case of combining each of Sample 1, the skin elasticity and moisture content are obviously improved, and the skin beauty is achieved by the combination of the collagen peptide and the blood flow improving agent according to the present invention, in particular in combination with Camellia extract, thioctal acid and resveratrol. It is clear that there is a function that significantly promotes the action.

[Table 5] Effect on human skin (mean value ± standard error)

Starting example 1 (soft capsule)

Sample 1 and Sample 3 (mixing ratio: 10/1), Sample 1 and Sample 7 (mixing ratio: 10/1), Sample 1 and thioctic acid (mixing ratio: 10/1), Sample 1 and Sample 3, and Sample 7 and One kind of thioctic acid (mixing ratio: 10/1/1/1): 200 parts of beeswax: 40 parts of beeswax and evening primrose oil (manufactured by UK EPA), added to the capsule filling machine after homogenization by heat mixing The gelatin-coated soft capsule formulation having a 250 mg content per tablet was started by a conventional method. This capsule formulation can be used as an orally ingestible nutritional supplement, medicine or animal feed.

Start example 2 (hard capsule)

Sample 1 and Sample 3 (mixing ratio: 10/1), Sample 1 and Sample 4 and Sample 6 (mixing ratio: 10/3/1), Sample 1 and Sample 4 and Sample 7 (mixing ratio: 10/1/1), Sample Any one of 1 and Sample 4 and Sample 5 and Sample 7 (mixing ratio: 10/2/1/1) was provided to the capsule charger to start a gelatin-coated hard capsule formulation having a content of 200 mg per tablet by a conventional method. This capsule formulation can be used as an orally ingestible nutritional supplement, medicine or animal feed.

Start example 3 (beverages)

In 100 mL of commercially available nutritional drinks, 1,000 mg of each of Sample 1 and Sample 3 (mixing ratio: 10/1), Sample 1 and Sample 7 (mixing ratio: 10/1), Sample 1, and thioctic acid (mixing ratio: 10/1) Each was added and thoroughly mixed to start a drink. This preserved in the refrigerator for 6 months, did not show any abnormalities and discomfort in appearance and flavor. This product can be used as a beverage or a drink for improving skin conditions such as drying, lowering elasticity, rough skin, and / or promoting skin cosmetology.

Industrial Applicability

A skin beauty promoter comprising as an active ingredient a combination comprising a collagen peptide of the present invention and one or two or more selected from the above-described three kinds of blood flow improving agents, dry or dry the skin by orally ingesting or administering it. Since it has the effect | action which reduces elasticity, improves skin roughness, and / or promotes skin beauty, it can utilize effectively in the field of food-drinks, medicines, quasi-drugs, feeds, etc.

Claims (13)

A skin beauty promoter comprising a collagen peptide and a blood flow improving agent as an active ingredient. The skin care promoter of claim 1, wherein the collagen peptide is a hydrolyzate of collagen or gelatin and has an average molecular weight of about 200 to about 10,000. The skin beauty promoter according to claim 1, wherein the blood flow improving agent is one or two or more selected from the group consisting of Camellia seed extract, thioctic acid, resveratrol and plant extract containing the resveratrol. The skin beauty promoter according to claim 3, wherein the camellia seed extract is an aqueous component obtained by extracting and treating the skim foil of the camellia seed with water and / or a lower alcohol. The method according to claim 3 or 4, wherein the Camellia seed extract contains saponins, wherein the saponins are Camelliasponin A1, Camelliasponin A2, Camelliasponin B1, Camelliasponin (Camelliasaponin) B2, Camelliasaponin (Celliasaponin) C1 and Camelliasponin (Camelliasaponin) C2 A skin cosmetic promoter comprising one or two or more selected from the group consisting of. The skin cosmetic promoter according to claim 3, wherein the thioctic acid is one or two or more selected from the group consisting of thioctic acid, dihydrothioctic acid, their salts, their esters, their amides and their cyclodextrin inclusions. 7. The crystal according to claim 3 or 6, wherein the thioctic acids are selected from the group consisting of thioctic acid, dihydrothioctic acid, their salts, their esters, their amides and their cyclodextrin inclusions. A skin beauty accelerator, which is formed by coating an outer surface of a powder and / or particles with lipids. The skin cosmetic promoter according to claim 6 or 7, wherein the thioctic acid and / or the dihydrothioctic acid are racemates. The skin cosmetic promoter according to claim 3, wherein the resveratrol is a composition comprising one or two or more selected from the group consisting of resveratrol, ε-viniferin, and isomers and / or glycosides thereof. The skin beauty promoter according to claim 3, wherein the resveratrol-containing plant extract is obtained by using stems, vines, shoots or flowers of grapes and plants belonging to the genus Grape. An oral composition comprising the skin care promoter of any one of claims 1 to 10. The oral composition according to claim 11, wherein the oral composition is a food or drink. A cosmetic method for improving skin condition such as drying of the skin, lowering of elasticity, roughness of the skin, and / or promoting skin beauty, characterized by orally ingesting the skin beauty promoter according to any one of claims 1 to 10. .