JP5016535B2 - Anti-aging food supplements and anti-aging agents - Google Patents

Description

  TECHNICAL FIELD The present invention relates to an anti-aging food supplement, and more particularly to an anti-aging food supplement, an anti-aging agent, a cell activator, a collagen production promoter, and a hyaluronic acid production promoter using Hanabiratake.

  One of the most common cosmetic problems is skin wrinkles and sagging. Such skin aging characterized by wrinkles and sagging of the skin is thought to be due to a decrease in collagen production ability and hyaluronic acid production ability of skin fibroblasts.

  Therefore, many researches and developments of anti-aging ingredients that effectively prevent and improve skin wrinkles and sagging have been made. For example, Patent Documents 1 and 2 disclose anti-aging skin external preparations containing plant extracts. Has been.

  However, when the anti-aging component is applied by percutaneous absorption like a skin external preparation, the absorbability becomes a problem, so that the effect as expected is often not achieved. On the other hand, in the case of oral intake such as food, since it is directly taken into the body, the effect of the intake is greater than that of external application.

  As a food that has been known to prevent aging in the past, for example, Patent Document 3 contains Tonaka, Ginseng, and Collagen as essential components, and by containing selected components such as deoxyribonucleic acid, metabolism of the living body, especially skin turnover. A skin activating food that promotes aging is described. Patent Document 4 describes a functional oral composition containing polyphenols and collagen derivatives and a degradation product of chitin, and Patent Document 5 describes an anti-aging food containing a stilbene compound.

On the other hand, the spatula crispula is a very rare mushroom belonging to the genus Hanabiratake, which grows on larch, red pine, beech and the like. An edible mushroom that is crunchy and features a pure white color and a leaf-peony shape.
As foods using the garlic bamboo, Patent Document 6 describes an ecologically active composition and an antitumor agent comprising an extract of the garlic bamboo as an active ingredient. Patent Document 7 discloses a functional food containing a component obtained from Hanabiratake, and Patent Document 8 discloses a collagen production promoter containing Hanabiratake or an extract thereof.

JP 2003-160433 A JP 2007-126368 A Japanese Patent No. 3308433 JP 2001-72582 A Japanese Patent Laid-Open No. 2001-69948 JP 2000-217543 A JP 2004-315512 A JP 2006-137680 A

  In view of the conventional circumstances as described above, an object of the present invention is to provide an anti-aging agent having a higher anti-aging effect with a small amount of ingestion.

  As a result of intensive studies in view of such circumstances, the present inventors have found that the food supplement containing Hanabiratake or its extract and resveratrol has excellent cell proliferating ability, and has excellent anti-aging by activating cells. The inventors have found that the present invention has an effect, and have completed the present invention.

That is, the present invention is an anti-aging food supplement characterized by comprising Hanabiratake or an extract thereof and resveratrol.
The food additive for anti-aging of the present invention preferably further comprises a collagen peptide having an average molecular weight of 5000 or less, and is preferably a food additive for cell utilization.

  Further, according to the present invention, an anti-aging agent, a cell activator, a collagen production promoter, and a hyaluronic acid production promoter, characterized in that they comprise Hanabiratake or an extract thereof, resveratrol and a collagen peptide having an average molecular weight of 5000 or less. Is provided.

The food supplement for anti-aging of the present invention is excellent in cell proliferation ability and can effectively suppress aging due to skin deterioration by activating cells.
The anti-aging agent of the present invention is excellent in cell activation action, collagen production promotion action, hyaluronic acid production promotion action, and as an anti-aging agent for preventing aging, as well as a cell activation agent, collagen production promotion agent, hyaluronic acid production promotion agent Can be used.

The best mode of the present invention will be described in detail below.
The food supplement for anti-aging according to the present invention comprises hanabiratake or an extract thereof and resveratrol.

(Hanabiratake)
Hanabiratake or extract thereof used in the present invention is a fruit body of Hanabiratake, a dried product thereof, and a pulverized product thereof, an extract obtained from the fruiting body of Hanabiratake, a product of dried mycelium, and a product thereof Any one of the pulverized product and the extract obtained from the mycelium of the moss, or a combination of two or more thereof.

  The fruit body of the flower is a natural one or may be obtained by artificial cultivation. Artificial cultivation can be performed by creating a fungal bed for artificial cultivation.

  In addition, the method for obtaining mycelium by cultivating Hanabiratake may be either a solid culture method or a liquid culture method usually used for culture of basidiomycetes, but the latter method is preferably used from the viewpoint of productivity. The culture medium only needs to contain various nutrients necessary for the growth of bacteria, and may be a normal medium formulation. That is, as the carbon source, any carbon source that can be assimilated such as glucose, sucrose, maltose, and starch can be used. As a nitrogen source, for example, ammonium sulfate salt, ammonium nitrate salt, urea, etc., as a natural complex nutrient source, for example, potato extract, carrot extract, malt extract, peptone, koji extract, yeast extract, yeast powder, etc. can be used, Other trace element inorganic salts, vitamins and the like necessary for growth may be added as appropriate.

  Cultivation is usually carried out under aerobic conditions. For example, a shaking culture method or an aeration stirring culture method is used. The agitation during the culture may be performed by reciprocal shaking or rotational shaking for several minutes every 24 hours, but may be continuously shaken. The culture temperature is preferably 15 ° C to 40 ° C, more preferably around 20 ° C to 30 ° C. The pH during the culture is preferably adjusted to a range of 3.0 to 9.0, more preferably 4.5 to 7.0. Moreover, although it is preferable not to illuminate during culture | cultivation, the illumination for about 11 to 14 hours a day is possible.

  The number of days of culture varies depending on the culture conditions such as the physical environment and the culture composition, but it is sufficient that the mycelium grows. Usually, the maximum mycelium is produced in 2 to 120 days, particularly preferably 5 to 90 days. The time is good.

  After completion of the culture, the mycelium and the culture filtrate are separated by centrifuging or filtering the culture liquid.

  As a method for drying the fruit body or mycelium of Hanabiratake obtained as described above, hot air drying, vacuum freeze drying, cold air drying, far-infrared drying and the like can be used, and hot air drying is desirable. Although dried Hanabira bamboo can be used as it is as the functional composition of the present invention, it is usually preferable to use a pulverized product or powder. As a method for pulverizing Hanabiratake, a stone pulverization method, a ball mill method, a collision pulverization method such as a jet mill, or the like can be used.

  In addition to the above, as for the moss bamboo or the extract thereof used in the present invention, a product (extract, etc.) extracted from the fruit body or mycelium of the moss mushrooms raw or dried with a solvent or the like after drying is also effective.

  There are no particular restrictions on the method used to obtain the extract from the fruit bodies or mycelia of the moss, but in addition to solvent extraction, supercritical extraction, physical extraction using a ball mill or blender, and an enzyme to increase extraction efficiency. The method of adding etc. can be combined suitably. As the solvent used for solvent extraction, an aqueous solvent or a non-aqueous solvent can be used. Specific examples of aqueous solvents include water itself, alkaline water to which alkali or other basic substances are added, aqueous solvents to which organic solvents compatible with alcohol, etc. are added, acidic water to which acids or acidic substances are added, organic An aqueous solution containing an acid (for example, lactic acid, acetic acid, etc.), an inorganic salt (for example, yeast food), or a mixture of two or more of these can be suitably used.

  Specific examples of non-aqueous solvents include ethyl alcohol, isopropyl alcohol, glycerin, propylene glycol, dimethyl sulfoxide, and dimethylformamide.

  Solvent extraction can be performed by the following operations. That is, 2 to 10 parts by mass of a solvent is added to 1 part by mass of freshly crushed or sliced fresh fruit or dried body thereof, preferably 40 to 120 ° C., more preferably about 80 ° C. for 2 hours or more or 90 ° C. For 30 minutes or longer. Thereafter, solid-liquid separation is performed, and the extract can be further concentrated.

  This solvent extraction process may be performed in multiple stages, or may be performed as a multistage process using different solvents. The amount of the solvent used is not particularly limited, but is preferably determined in consideration of the weight of the target bamboo shoot, the weight of the solvent or residue, and the post-treatment burden.

  The food additive for anti-aging of the present invention is prepared so that it can be taken in an amount of 0.0025 g or more, preferably 0.003 g or more per day, in terms of solid content, obtained from the above-mentioned method. Is desirable.

(Resveratrol)
Resveratrol used in the present invention is stilbenetriol, and is known to have a skin aging preventive action and an oral disease healing action (for example, JP 2002-293736 A, JP 2001-2001 A). No. 69948, JP-A 2000-256154).

As the resveratrol used in the present invention, a resveratrol derived from grape is preferable, and as this resveratrol, for example, VINEATROL 20M manufactured by Sunbright Co., Ltd. may be mentioned.
VINEATROL 20M is a grapevine shoot-derived resveratrol (monomers and oligomers), which contains transresveratrol> 5%, ε-viniferin> 5%, total resveratrol (monomers and oligomers)> 20% Is.

  The anti-aging food supplement of the present invention is preferably prepared so that resveratrol can be taken 0.0001 g or more, preferably 0.0006 g or more per day.

  In the food supplement for anti-aging of the present invention, the intake ratio of the garlic bamboo or its extract and resveratrol is preferably garlic or the extract: resveratrol = (3: 1) to (7: 1), More preferably, it is (4: 1) to (6: 1).

[Collagen peptide]
The food additive for anti-aging of the present invention exhibits an anti-aging effect only with an essential component, Hanabiratake or its extract and resveratrol, but preferably further contains a collagen peptide. By containing a collagen peptide, the anti-aging effect is increased even if the amount of each component is small.

The collagen peptide is preferably a hydrolyzate of fish-derived collagen. Examples of the hydrolyzate of collagen derived from fish include HACP-U2 manufactured by Zerais Co., Ltd.
The collagen peptide used in the present invention has an average molecular weight of 5000 or less.

  The anti-aging food supplement of the present invention is preferably prepared so that 0.005 g or more, preferably 0.03 g or more of collagen peptide can be ingested per day.

  In the food additive for anti-aging of the present invention, the intake ratio of collagen peptide and resveratrol is preferably collagen peptide: resveratrol = (30: 1) to (70: 1), more preferably (40: 1) to (60: 1).

  The anti-aging agent, cell activator, collagen production promoter and hyaluronic acid production promoter of the present invention are all composed of Hanabiratake or an extract thereof, resveratrol, or a collagen peptide having an average molecular weight of 5000 or less. Although what consists of what added is preferable, it may contain the other component.

[Other ingredients]
In addition to the essential components, additives can be arbitrarily selected and used in combination with the anti-aging food supplement of the present invention as necessary. Additives can include excipients, flavoring agents, coloring agents, preservatives, thickeners, stabilizers, gelling agents, antioxidants, functional materials, and the like.
Functional materials include various vitamins, pantothenic acid, folic acid, biotin, zinc, calcium, magnesium, amino acids, oligosaccharides, propolis, royal jelly, EPA, DHA, coenzyme Q10, chondroitin, lactic acid bacteria, lactoferrin, isoflavones, prunes, chitin , Chitosan, glucosamine and the like. These functional materials can be used alone or in combination of two or more.

  The excipient may be anything that is normally used in the desired dosage form, for example, powders such as fine particle silicon dioxide, sucrose fatty acid ester, crystalline cellulose / sodium carboxymethylcellulose, hydrogen phosphate Calcium, wheat starch, rice starch, corn starch, potato starch, dextrin, starch such as cyclodextrin, crystalline cellulose, lactose, glucose, sugar, reduced maltose, syrup, sugarcane, fructooligosaccharide, emulsified oligosaccharide, sorbitol, Examples thereof include sugar alcohols such as erythritol, xylitol, lactitol, mannitol. These excipients can be used alone or in combination of two or more.

  As a flavoring agent, Bontan extract, lychee extract, apple juice, orange juice, yuzu extract, peach flavor, ume flavor, sweeteners acesulfame K, erythritol, oligosaccharide, mannose, xylitol, isomerized sugar Green tea, oolong tea, banaba tea, Tochu tea, iron kannon tea, pearl barley tea, amacha tea, macomo tea, kelp tea, yogurt flavor and the like. These taste agents can be used alone or in combination of two or more.

  Other colorants, preservatives, thickeners, stabilizers, gelling agents, and antioxidants can be appropriately selected from known ones used in foods.

  The form of the anti-aging food supplement of the present invention can be any ampule, capsule, pill, tablet, powder, granule, solid, liquid, gel or foam, cream and the like.

Specifically, beauty / health drink or food (vitamin supplementation, nourishing tonic, recovery from fatigue, improvement of rough skin, improvement of body structure, beauty hair, hair growth / hair growth), pharmaceuticals, confectionery, Japanese confectionery, gum, candy, caramel, etc. General soft drinks such as fruit juices, processed soft drinks such as kamaboko and chikuwa, livestock products such as sausages and ham, raw noodles, boiled noodles, buckwheat noodles, sauces, soy sauce, sauce, sugar, Seasonings such as honey, powdered candy, syrup, etc., spices such as curry powder, mustard powder, pepper powder, dairy products such as jam, marmalade, chocolate spread, cheese, butter, yogurt, beauty / health cream, beauty / health liquid Products, beauty / health fopplies, etc.
Moreover, these can be manufactured by a conventionally well-known method.

  The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto.

Test Example 1 (Measurement of cell proliferation ability)
Human dermal fibroblasts were seeded on a 96-well microplate at a concentration of 2 × 10 ³ cells / well in 100 μl of a medium containing the samples of the following test examples (1-1) to (1-8), and incubated at 37 ° C. It was incubated for 72 hours at 6.5% CO _2.
After 72 hours, using Roche's Cell Proliferation Kit I Cell Proliferation Kit I, 10 μL of MTT labeling reagent (final concentration: 0.5 mg / ml) in the kit was added to each well, and the mixture was incubated at 37 ° C., 6.5%. Plates were incubated for 4 hours with CO ₂ .
After the 4 hour incubation, 100 μl of solubilization solution was added to each well and the plate was left overnight at 37 ° C. and 6.5% CO ₂ .
The complete solubilization of the purple formazan crystals was checked, and the absorbance of the sample (measurement wavelength 580 nm, reference wavelength 650 nm) was measured with a plate reader.

  Samples are as follows. As resveratrol, a reagent manufactured by Tokyo Chemical Industry Co., Ltd. was purchased and used as a habitat bamboo, and a commercially available average molecular weight of 5000 or less was used as a collagen peptide. The same applies to the following examples. In addition, about the algal bloom, the value which converted the sample density | concentration of the algal bloom extract into the real part was described.

(Preparation of Hanabiratake extract)
Hanabiratake is dried and powdered, and 10 g of Hanabiratake is suspended in 100 mL of PBS (−). After heat extraction at 100 ° C. for 2 hours, and further 2 hours later, the temperature of the extract was lowered to around room temperature, and then sterilized by filtration using a 0.45 μm filter to obtain an extract of Hanabiratake. (Hanabiratake powder 10g / 100mL PBS (-), heat-extracted amount 1.22g / 10g Hanabiratake powder)

Test Example 1-1: Control Test Example 1-2: Resveratrol (1 μM)
Test Example 1-3: Collagen peptide (10 μg / mL)
Test Example 1-4: Hanabiratake (1.22 μg / mL)
Test Example 1-5: Collagen peptide (5 μg / mL) + Hanabiratake (5 μg / mL)
Test Example 1-6: Collagen peptide (5 μg / mL) + resveratrol (0.5 μM)
Test Example 1-7: Hanabira bamboo (0.61 μg / mL) + resveratrol (0.5 μM)
Test Example 1-8: Hanabiratake (0.41 μg / mL) + resveratrol (0.3 μM) + collagen peptide (3.3 μg / mL)

(Evaluation results)
FIG. 1 is a graph showing the results of a cell proliferation ability test in human dermal fibroblasts of the cell activator (anti-aging agent) obtained in Test Examples 1-1 to 1-8.
From the results of FIG. 1, it can be seen that the cell activator (anti-aging agent) of the present invention is excellent in cell proliferation ability and can suppress aging due to skin deterioration. A large synergistic effect is observed particularly when containing Hanabiratake, resveratrol and collagen peptide (Test Example 1-8).

Test Example 2 (Measurement of collagen production ability)
Human dermal fibroblasts were cultured in 24-multiwell until confluent, and the culture solution was replaced with the treatment solution shown below. After treatment for 72 hours, the culture solution was collected. The collected culture medium was measured for the amount of collagen in the culture medium using procollagen type I C-peotide (PIP) EIA kit (TaKaRa).

(Treatment solution)
Test Example 2-1: Control Test Example 2-2: Resveratrol (1 μM)
Test Example 2-3: Collagen peptide (10 μg / mL)
Test Example 2-4: Hanabiratake (10 μg / mL)
Test Example 2-5: Collagen peptide (5 μg / mL) + Hanabiratake (5 μg / mL)
Test Example 2-6: Collagen peptide (5 μg / mL) + resveratrol (0.5 μM)
Test Example 2-7: Hanabiratake (5 μg / mL) + resveratrol (0.5 μM)
Test Example 2-8: Hanabira bamboo (3.3 μg / mL) + resveratrol (0.3 μM) + collagen peptide (3.3 μg / mL)

FIG. 2 is a graph showing the results of a collagen production ability test of the collagen production promoter (anti-aging agent) obtained in Test Examples 2-1 to 2-8.
From the results of FIG. 2, it can be seen that the collagen production promoter (anti-aging agent) of the present invention is excellent in collagen production ability and can suppress aging due to skin deterioration. In particular, a large synergistic effect is observed in the case of containing the bamboo shoot, resveratrol and collagen peptide (Test Example 2-8).

Test Example 3 (Measurement of hyaluronic acid production ability)
Human dermal fibroblasts were cultured in 24-multiwell until confluent, and the culture solution was replaced with the treatment solution shown below. After treatment for 72 hours, the culture solution was collected. The collected culture solution was measured for the amount of hyaluronic acid in the culture solution using Hyaluronan Assay kit (Seikagaku Corporation).

(Treatment solution)
Test Example 3-1: Control Test Example 3-2: Resveratrol (1 μM)
Test Example 3-3: Collagen peptide (10 μg / mL)
Test Example 3-4: Hanabiratake (10 μg / mL)
Test Example 3-5: Collagen peptide (5 μg / mL) + Hanabiratake (5 μg / mL)
Test Example 3-6: Collagen peptide (5 μg / mL) + resveratrol (0.5 μM)
Test Example 3-7: Hanabiratake (5 μg / mL) + resveratrol (0.5 μM)
Test Example 3-8: Hanabira bamboo (3.3 μg / mL) + resveratrol (0.3 μM) + collagen peptide (3.3 μg / mL)

FIG. 3 is a graph showing the results of the hyaluronic acid production ability test of the hyaluronic acid production promoter (anti-aging agent) obtained in Test Example 3.
From the results of FIG. 3, it can be seen that the hyaluronic acid production promoter (anti-aging agent) of the present invention is excellent in hyaluronic acid production ability and can suppress aging due to skin deterioration. In particular, hyaluronic acid production promoters (anti-aging agents) including hanabiratake, resveratrol and collagen peptides have a large synergistic effect on hyaluronic acid production ability.

Formulation Example 1: Candy 4000 mg / day sugar 2300 mg
Minamata 1486
Collagen peptide 25
Hanabira bamboo powder 12.5
Resveratrol 0.5
Fragrance 38

Formulation Example 2: Tablet 1500 mg / day sucrose ester 70 mg
Crystalline cellulose 74
Methylcellulose 36
Glycerin 28
Collagen peptide 600
Hanabira Bamboo Powder 60
Resveratrol 12
N-acetylglucosamine 100
Hyaluronic acid 50
Vitamin E 30
Vitamin B6 20
Vitamin B2 10
α-Lipoic acid 20
Coenzyme Q10 40
Ceramide (konjac extract) 50
L-proline 300

Formulation Example 3: Soft Capsule 1500mg / Soybean Soybean Oil 324mg
Collagen peptide 300
Hanabira Bamboo Powder 30
Resveratrol 6
Royal Jelly 150
Maca 30
GABA 30
Beeswax 30
Gelatin 375
Glycerin 120
Glycerin fatty acid ester 105

Formulation Example 4: Soft Capsule 1500mg / Nippon Rice Germ Oil 659mg
Collagen peptide 50
Hanabira Bamboo Powder 500
Resveratrol 1
Lotus germ extract 100
Elastin 130
DNA 30
Folic acid 30

Formulation Example 5: Granule 1200 mg / day collagen peptide 500 mg
Hanabira bamboo powder 100
Resveratrol 10
Soy isoflavone 50
Reduced lactose 395
Soybean oligosaccharide 36
Erythritol 36
Dextrin 25
Citric acid 24
Fragrance 24

Formulation Example 6: Drink 50 mL / day collagen peptide 600 mg
Hanabira Bamboo Powder 60
Resveratrol 12
N-acetylglucosamine 100
Hyaluronic acid 50
Vitamin E 30
Vitamin B6 20
Vitamin B2 10
α-Lipoic acid 20
Coenzyme Q10 40
Ceramide (konjac extract) 50
L-proline 300
Drink base 4500
(Including reduced maltose starch syrup, erythritol, citric acid, and fragrance)
Purified water A total volume of 50 mL

It is a graph which shows the result of the cell growth ability test using the human skin dermal fibroblast of the cell activator (anti-aging agent) of this invention. It is a graph which shows the result of the collagen production ability test using the human skin dermal fibroblast of the collagen production promoter (anti-aging agent) of this invention. It is a graph which shows the result of the hyaluronic acid production ability test using the human skin dermal fibroblast of the hyaluronic acid production promoter (anti-aging agent) of this invention.

Claims (3)

  A cell activator characterized by comprising Hanabiratake or an extract thereof, resveratrol, and a collagen peptide having an average molecular weight of 5000 or less.   A collagen production promoter, characterized by comprising Hanabiratake or an extract thereof, resveratrol, and a collagen peptide having an average molecular weight of 5000 or less.   A hyaluronic acid production promoter characterized by consisting of Hanabiratake or an extract thereof, resveratrol and a collagen peptide having an average molecular weight of 5000 or less.

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