JP6085137B2 - Anti-aging agent - Google Patents

Description

  The present invention relates to an anti-aging agent.

  Conventionally, a method is known in which fish ovary membrane (egg skin) is previously treated with ozone water, and then myofibrillar protein, which is a constituent protein, is enzymatically decomposed to extract amino acids and peptides (for example, patents). Reference 1).

  The amino acids and peptides can be used as physiologically active substances or as food fortifiers. More specifically, the amino acids and peptides have ACE inhibitory activity and act as blood pressure increase inhibitors (hypertensive agents).

  On the other hand, it is known that the components extracted by treating the ovary membrane of sputum with a proteolytic enzyme are effective as anti-aging agents for improving rough skin, increasing IGF-1 value, increasing acceleration pulse wave aging index, etc. (For example, see Patent Documents 2 to 4).

JP 2004-73186 A Japanese Patent No. 3899116 Japanese Patent No. 3946238 Japanese Patent No. 3946239

  It has been confirmed by customer questionnaires that the components extracted by treating the ovary membrane of the pupae with proteolytic enzymes are effective for the prevention of aging. Anti-aging agents with even better effects are desired.

  In view of such circumstances, an object of the present invention is to provide an anti-aging agent that contains a component extracted by treating the ovarian membrane of sputum with a proteolytic enzyme and can obtain further excellent effects.

To achieve the above object, an anti-aging agent of the present invention, the component extracted by treating the ovarian membrane salmon with proteolytic enzymes, and food to be added pharmacologically active ingredient, It contains collagen, nucleic acid, elastin, hyaluronic acid, ceramide, and galactooligosaccharide .

  In the living body, with age, shoulder stiffness, joint pain, swelling, coldness, anemia, decline in metabolism, lethargy, easy fatigue, poor appetite, poor sleep, poor sleep, tired eyes , Various symptoms such as dry mouth, rough skin texture, no gloss, no wrinkles, deep wrinkles, more spots, freckles, dullness, pore sagging, pore opening, dryness, etc. Symptoms such as loss of hair shine and hair stiffness appear. In women, symptoms such as sweating, irritation, menstrual pain and irregular menstrual symptoms, and premenstrual syndrome also occur.

  The anti-aging agent of the present invention contains a pharmacologically effective component together with a component extracted by treating the ovary membrane of vagina with a proteolytic enzyme (hereinafter abbreviated as vaginal ovary membrane extract component). In the alleviation of various symptoms associated with aging, an effect superior to that obtained when the ovary membrane extract component is used alone can be obtained.

  The anti-aging agent of the present invention preferably contains the vaginal ovary membrane extract component in a range of 1.0 to 65% by mass with respect to the total amount with the pharmacologically effective component. When the content of the ovary membrane extract component is less than 1.0% by mass with respect to the total amount with the pharmacologically effective component, the effect of the ovary membrane extract component itself cannot be sufficiently obtained. Sometimes. Further, even if the content of the ovary membrane extract component exceeds 65% by mass with respect to the total amount with the pharmacologically effective component, no further effect may be obtained.

Moreover, anti-aging agent of the present invention, as the pharmacologically active ingredient, collagen and nucleic acids and, elastin and, hyaluronic acid, ceramide, including a galactooligosaccharide.

  In this case, the anti-aging agent of the present invention is, for example, the above-mentioned ovarian membrane extract component 100 to 500 mg, collagen 200 to 2000 mg, nucleic acid 50 to 1000 mg, elastin 5 to 50 mg, hyaluronic acid 0.5 to 20 mg, ceramide 5 to 50 mg. , Which may contain 50 to 3000 mg of galactooligosaccharide.

The graph which compares the physical condition and constitution after ingesting the anti-aging agent of this invention for 8 weeks with the physical condition and constitution after ingesting the anti-aging agent which consists only of a vaginal ovary membrane extract component for 8 weeks. The graph which compares the skin state after ingesting the anti-aging agent of this invention for 8 weeks with the skin state after ingesting the anti-aging agent which consists only of a vaginal ovarian membrane extract component for 8 weeks.

  Next, embodiments of the present invention will be described in more detail with reference to the accompanying drawings.

  The anti-aging agent of this embodiment contains the ovary membrane extract component and the pharmacologically effective component that can be added to food.

  The salmon ovary membrane extract component is obtained by, for example, a method of filtering a solution obtained by treating a ovary membrane isolated from salmon fish eggs with a proteolytic enzyme and extracting the protein, and drying the obtained filtrate. Can do.

  Specifically, in the above method, first, the ovarian membrane of the pupa is used as a raw material, and water is added to the ovarian membrane at a mass ratio of ovarian membrane: water = 1: 1 to 1: 3, and the mixture is stirred and mixed. Further, the proteolytic enzyme is added in the range of 1 to 3% by mass with respect to the total amount of the ovarian membrane, and heated at a temperature in the range of 45 to 55 ° C. for 30 minutes to 5 hours, preferably 2 hours. If it does in this way, the component decomposed | disassembled with the said proteolytic enzyme will elute in water among the components of the said ovary membrane, and the aqueous solution of this component will be obtained.

  Next, the proteolytic enzyme contained in the aqueous solution is inactivated. The deactivation can be performed, for example, by heating the aqueous solution at a temperature of 90 ° C. for 5 minutes.

  Next, the aqueous solution is simply filtered through a wire mesh of about 30 mesh to remove coarse materials such as undecomposed ovarian membranes. Then, activated carbon is added to the obtained filtrate to deodorize, decolorize, and degrease the filtrate. Deodorization, decolorization, and degreasing of the filtrate are performed by adding activated carbon in the range of 2 to 4% by mass to the total amount of the ovarian membrane as the raw material and heating the filtrate at a temperature of 60 ° C. for 30 minutes, for example. be able to.

  After the deodorization, decolorization, and degreasing treatment with the activated carbon, the filtrate is filtered by, for example, a filter press, and the obtained filtrate is concentrated at a temperature of 60 ° C. under reduced pressure, for example, and then maintained at a temperature of 80 ° C. for 10 minutes, for example. And sterilize. Then, the ovary membrane extract component can be obtained by drying the sterilized filtrate by spray drying. The ovary membrane extract component contains amino acids, peptides, vitamins, minerals, sugars, enzymes, nucleic acids and their metabolites, various growth factors, cytokines, and the like.

  Next, pharmacologically effective ingredients that can be added to the food include, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid. , L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan, casein , Gelatin, pectin, agar, vitamin B (B1, B2, B6, B12, etc.), nicotinamide, calcium pantothenate, amino acids, calcium salts, reduced ascorbic acid (vitamin C), reduced gluta , Tocotrienol, vitamin A, vitamin A derivative, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sesamin, lignans, catechin, isoflavone, chalcone, Tannins, flavonoids, coumarins, isocoumarins, blueberry extract, vitamin D, vitamin P, choline, niacin, pantothenic acid, calcium folate, fish oil, EPA, oligosaccharides, galactooligosaccharides, dietary fiber, squalene, soy lecithin, taurine, Donariella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster meat extract, chitosan Chitin oligosaccharide, collagen, chondroitin sulfate, proteoglycan, non-denatured type II collagen, mucopolysaccharide, elastin, turmeric, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, swordfish extract, suppon, seiyou, plantain, chamomile, chamomile , Dandelion, hibiscus, honey, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract , Brown rice germ extract, onion, DPA, green tea, cordyceps, garlic, bee, papaya, puer, propolis, magnoli tree, jabushitake, royal jelly, saw palmetto, hyaluronic acid, gabba, harp seal oil, sa Cartilage, glucosamine, lecithin, phosphatidylserine, tannin carrot, mulberry leaves, soy extract, echinacea, elegans, barley extract, olive leaf, olive fruit, gymnema, banaba, salasia, garcinia, St. John's wort, jujube, carrot, Passion flower, broccoli, placenta, placenta extract, placenta extract powder, placenta extract, pearl barley, grape seeds, peanut seed coat, bilberry, black cohosh, maria thistle, laurel, rafma, black vinegar, bitter gourd, maca, safflower, flax, oolong tea, Flower spines, caffeine, capsaicin, xylo-oligosaccharide, buckwheat, citrus, prunes, spirulina, barley young leaves, nucleic acid, yeast, shiitake, plum meat, deep sea potato extract, noni, oyster meat, champignon, acerola, pineapple, banana, mo Apricot, Melon, Strawberry, Raspberry, Orange, Fucoidan, Meshima Cobb, Cranberry, Iron, Silk Peptide, Glycine, Chest Tree, Ceramide, L-cysteine, Red Wine Juice, Millet, Horsetail, Biotin, Centella Asiatica, Hascup, French coastal pine bark extract powder, Japanese cypress, rhubarb, clove, brewer's yeast, Merirot, black zinger, gadget, nattokinase, benichouji, buckwheat, cocoa, dokudami, kiwi, hihatu, lotus leaf, puffer, star fruit, lycopene, les Examples include veratrol.

  It is preferable that the anti-aging agent of this embodiment contains the said ovary membrane extract component in 1.0-65 mass% or more with respect to the total amount with the said pharmacologically effective component.

  The anti-aging agent of the present embodiment can be used by arbitrarily selecting a pharmacologically effective ingredient that can be added to the food together with the vaginal ovary membrane extract component, for example, collagen, nucleic acid, elastin, and the like. Hyaluronic acid, ceramide, and galactooligosaccharide can be used. In this case, the anti-aging agent of the present invention is, for example, the above-mentioned ovarian membrane extract component 100 to 500 mg, collagen 200 to 2000 mg, nucleic acid 50 to 1000 mg, elastin 5 to 50 mg, hyaluronic acid 0.5 to 20 mg, ceramide 5 to 50 mg. , Which may contain 50 to 3000 mg of galactooligosaccharide.

  Examples of the anti-aging agent of the present embodiment include edible oil (such as salad oil), confectionery (such as gum, candy, caramel, chocolate, cookie, snack, jelly, gummi, and tablet confectionery), noodles (soba, udon, ramen, etc.) ), Dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) It can be used as a raw material for health foods (tablets, capsules, etc.), nutritional supplements (nutrient drinks, etc.).

  When the anti-aging agent of this embodiment is used as a health food, it can be administered orally in dosage forms such as tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids and the like. When used in the form of the tablet, pill, fine granule, powder, granule, etc., the anti-aging agent of the present embodiment further comprises excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, Kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin, talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinyl Pyrrolidone, etc.), disintegrating agents (sodium alginate, agar, sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cacao) Fat, hydrogenated oil, etc.), absorption enhancers (fourth Contains ingredients such as ammonium base, sodium lauryl sulfate), adsorbents (glycerin, starch, lactose, kaolin, bentonite, succinic acid, etc.), lubricants (purified talc, stearate, polyethylene glycol, etc.) Also good.

  In addition, the anti-aging agent of this embodiment is good also as not only oral administration but parenteral administration. For parenteral administration, use in dosage forms such as haps, lotions, ointments, tinctures, creams, etc. in the state of solutions or with the addition of dispersants, suspensions, stabilizers, etc. Can do.

  Next, examples and comparative examples of the present invention are shown.

〔Example〕
In this example, first, an anti-aging agent was prepared by formulating a vaginal ovary membrane extract component and a pharmacologically effective component in the form of a tablet. The anti-aging agent comprises 490 parts by weight of a vaginal ovary membrane extract component, 200 parts by weight of collagen as a pharmacologically effective ingredient, 100 parts by weight of nucleic acid, 10 parts by weight of hyaluronic acid, 10 parts by weight of ceramide, 10 parts by weight of elastin, It contains 50 parts by mass of galactooligosaccharide, 120 parts by mass of dextrin as an excipient, and 10 parts by mass of calcium stearate. The tablet has a mass of 250 mg per tablet and is formulated to have a diameter of 9 mm.

  Next, 10 healthy female monitors in their late 30s to 40s were allowed to take 4 tablets per day for 8 weeks as a health food. Each monitor has not taken supplements or medicines (including herbal medicines) since one month ago.

Then, 8 weeks after the start of ingestion, changes in physical condition / physical constitution and skin condition were investigated by self-reporting of each monitor, and the results were scored. FIG. 1 shows the average value of each monitor for changes in physical condition and constitution, and FIG. 2 shows the average value of each monitor for changes in skin condition.
[Comparative Example]
In this comparative example, first, instead of the anti-aging agent of the above-mentioned example, 980 parts by mass of a vaginal ovary membrane extract component, 18.5 parts by mass of dextrin as an excipient and 1.5 parts by mass of calcium stearate are included. Anti-aging tablets were produced. The tablet does not contain any pharmacologically active ingredients other than the vaginal ovary membrane extract ingredient, has a mass of 250 mg per tablet, and is formulated in a diameter of 9 mm.

  Next, except for using the anti-aging agent of this comparative example, the change in physical condition / physical condition and skin condition of 10 female monitors was investigated in exactly the same manner as in the above example, and the results were scored. FIG. 1 shows the average value of each monitor for changes in physical condition and constitution, and FIG. 2 shows the average value of each monitor for changes in skin condition.

  From FIG. 1 and FIG. 2, according to the anti-aging agent of the above example, the total amount of the ovarian membrane extract component and the pharmacologically effective component is contained in the anti-aging agent of the comparative example. In spite of being less than the amount of the extracted component, it is clear that the effect of the anti-aging agent of the comparative example is superior in improving the physical condition, constitution and skin condition.

  In addition, according to the results of a questionnaire survey of the 10 female monitors, it was confirmed that the anti-aging agent of the above example was effective in treating and improving osteoporosis, osteoarthritis, and joint pain.

Claims (3)

A component extracted by treating the ovarian membrane salmon with proteolytic enzymes, and food to be added pharmacologically active ingredient, the collagen, and the nucleic acid, and elastin, hyaluronic acid, ceramide, An anti-aging agent comprising galactooligosaccharide .   2. The anti-aging agent according to claim 1, wherein a component extracted by treating the ovarian membrane of sputum with a proteolytic enzyme is 1.0 to 65 with respect to a total amount of the pharmacologically effective component. An anti-aging agent characterized by being contained in a mass% range. The anti-aging agent according to claim 1 or 2 , wherein the ovary membrane of the pupae is extracted by treating the ovarian membrane with a proteolytic enzyme, 100 to 500 mg, collagen 200 to 2000 mg, nucleic acid 50 to 1000 mg, elastin 5 to 50 mg. An anti-aging agent comprising 0.5 to 20 mg of hyaluronic acid, 5 to 50 mg of ceramide, and 50 to 3000 mg of galactooligosaccharide.

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