JP2016124832A - Energy metabolism activator in muscle cells - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel muscle energy metabolism activator or the like, and furthermore, a composition that can be taken as medicine or food.SOLUTION: Technical features of the present invention for solving the problems above are as follows: (1) a sugar transporter gene expression promoter in muscle cells which has black ginger and/or extract thereof as an active ingredient; (2) a lactic acid transporter gene expression promoter in muscle cells which has black ginger and/or extract thereof as an active ingredient; (3) a sugar uptake promoter in muscle cells which has black ginger and/or extract thereof as an active ingredient; (4) a lactic acid uptake promoter in muscle cells which has black ginger and/or extract thereof as an active ingredient; and (5) an energy metabolism activator in muscle cells which has black ginger and/or extract thereof as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a novel muscle energy metabolism activator. The present invention is widely used for foods and drinks, pharmaceuticals, quasi drugs, and the like.

It is known that a large amount of energy is consumed in muscle during exercise, and most of the energy source depends on sugar (such as glucose). Thus, sugar uptake in muscle plays an important role in energy production. Therefore, an increase in the amount of sugar taken into the muscle can be expected to improve performance during exercise (see Non-Patent Document 1).
Here, the sugar transporter (GLUT4) is involved in the uptake of sugar into muscle cells.

Also, during exercise, energy production in the muscle depends mainly on the glycolytic system, and most of the sugar that is the energy source is metabolized to lactic acid and accumulates in the muscle. Lactic acid accumulation in muscle is thought to cause muscle pain, fatigue, and stiffness after exercise (lactic acidosis). However, recent studies have shown that lactic acid is not a causative agent of fatigue in muscle but a valuable energy source. Therefore, an increase in the amount of lactic acid taken up by muscle cells has a great effect on improving endurance and reducing fatigue after exercise (see Non-Patent Document 1). Here, the lactic acid transporter (monocarbic acid transporter MCT1) is involved in the uptake of lactic acid into muscle cells.

In addition, conventional muscle strength enhancers increase muscle strength by increasing muscle cells themselves, but simply growing muscle cells is not enough to make a good quality muscle, and even higher quality In order to make muscle, it was necessary to improve the efficiency of energy metabolism of muscle cells.

Sports training utilizing energy metabolism

Under such a background, the present inventor promoted the expression of the sugar transporter gene and the lactate transporter gene in muscle cells in black ginger and its extract, and thereby the action of incorporating sugar and lactate into muscle cells. As a result, it was found that it has an action of activating energy metabolism in muscle cells, and the present invention was completed.
That is, an object of the present invention is to produce a muscle having excellent quality by providing a novel energy metabolism activator in muscle cells.

The technical features of the present invention for solving the above-described problems are as follows.
(1) A sugar transporter gene expression promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient.
(2) A lactate transporter gene expression promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient.
(3) A sugar uptake promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient.
(4) A lactic acid uptake promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient.
(5) An energy metabolism activator in muscle cells containing black ginger and / or its extract as an active ingredient.

It is a graph which shows the evaluation result of the expression level of the sugar transporter GLUT4 in the muscle cell in a black ginger extract. It is a graph which shows the evaluation result of the expression level of the lactate transporter MCT1 in the muscle cell in a black ginger extract. It is a graph which shows the result of the uptake | capture amount of the saccharide | sugar in the muscle cell in a black ginger extract. It is a graph which shows the result of the amount of lactic acid in a muscle cell and a culture medium.

The present invention is described in detail below.
The sugar uptake promoter, the lactic acid uptake promoter, and the energy metabolism activator in muscle cells (hereinafter collectively referred to as “energy metabolism activator etc.”) of the muscle cell of the present invention are black ginger and / or The extract is used as an active ingredient.
The “black ginger” used in the present invention is a plant having the scientific name Kaempferia parviflora, which is distributed in Southeast Asia and belongs to the genus Kaempferia.

Used in traditional medicine such as Thailand and Laos to improve energy, improve nutrition, lower blood sugar levels, recover physical strength, improve digestive system, vaginal belt, hemorrhoids, hemorrhoids, nausea, stomatitis, joint pain, gastric pain, etc. Has been.

The black ginger used in the present invention uses a rhizome. The form of black ginger is not particularly limited, and any of immature rhizomes, fully ripe rhizomes, dry rhizomes, and the like may be used. It is also preferable to use a juice obtained by squeezing the rhizome. The form of the squeezed liquid is not particularly limited, and may be either liquid or concentrated and dried.

However, in the case of raw rhizomes or juices, care must be taken for storage, and the rhizomes are most preferably sliced and dried.

When using the dried dried rhizome, it is preferable to pulverize the rhizome to about 40 mesh in advance with a pulverizer or the like in order to increase the extraction efficiency.

The solvent and temperature conditions used for extraction are not particularly limited, and can be arbitrarily selected and set. As the extraction solvent, a non-organic solvent such as water, an acid, or a base, or an organic solvent such as a hydrophilic solvent or acetone can be selected. As the hydrophilic solvent, at least one selected from the group of lower alcohols consisting of methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol and butyl alcohol is preferable from the viewpoint of operability and extraction efficiency. However, extraction with a non-organic solvent is preferable rather than extraction with an organic solvent, and water, warm water or hot water, water with a slight addition of acid, and ethanol are particularly preferable.

The acid used at this time is not particularly limited. However, the use of acetic acid is preferred from the viewpoint of availability, safety, and post-treatment.

Further, in the above extraction, it is preferable to repeat the extraction step once or more for the extraction residue. According to this method, the extraction efficiency can be improved. The solvent used for extraction in this case may be the same or different.

The above extract can be used as it is, but it is more preferable to remove the insoluble substances and the solvent by performing treatments such as filtration, centrifugation and fractional distillation. By performing such treatment, the purity becomes higher and the application range becomes wider.
Thereafter, the extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method to obtain an energy metabolism activator or the like.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

The extract and its fraction can be used as they are, but can also be used after being dried and powdered by means such as spray drying or freeze drying, if necessary.

  The energy metabolism activator of the present invention can be used as a material for various foods and drinks. Examples of the food and drink include edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummy, tablet confection etc.), noodles (soba, udon, ramen etc.), and dairy products ( Milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and health foods (tablets , Capsules, etc.) and nutritional supplements (nutrient drinks, etc.). The energy metabolism activator of the present invention and the like may be appropriately blended with these foods and drinks.

  These foods and drinks can be blended with various ingredients depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. In addition, this energy metabolism activator with health maintenance function includes other antioxidants and health food ingredients such as antioxidants (reduced ascorbic acid (vitamin C), vitamin E, reduced) Type glutatin, tocotrienol, vitamin A derivatives, lycopene, lutein, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sesamin, lignans, catechin, isoflavone, chalcone, tannins, flavonoids , Coumarin, isocoumarins, blueberry extract), health food ingredients (V. (vitamin) A, V.B1, V.B2, V.B6, V.B12, VC, VD, VE, VP, choline, niacin, pantothene) Acid, calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soybean lecithin, taurine, Nariella, protein, octacosanol, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, chromium, selenium, potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, keihi, hawthorn, ginger , Reishi, swordfish extract, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, plantain, chamomile, chamomile, dandelion, hibiscus, honey, boren, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria , Faecalis, lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA, DPA, salmon tea Cordyceps, garlic, bee, papaya, puer, propolis, mugwort tree, jabushitake, royal jelly, saw palmetto, hyaluronic acid, collagen, gabba, harpseal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, paddy carrot, Mulberry leaves, soy extract, echinacea, sorghum, barley extract, olive leaf, olive fruit, gymnema, banaba, salacia, garcinia, chitosan, st jones wort, jujube, carrot, passionflower, broccoli, placenta, pearl barley, grape seeds, Peanut seed coat, bilberry, black cohosh, maria thistle, laurel, sage, rosemary, ruffa, black vinegar, bitter gourd, maca, safflower, flax, oolong tea, flower spine, caffeine, capsaicin, xylori Sugar, Glucosamine, Buckwheat, Citrus, Dietary fiber, Protein, Prunes, Spirulina, Barley young leaves, Nucleic acid, Yeast, Shiitake, Plum meat, Amino acid, Deep-sea coconut extract, Noni, Oyster meat, Suppon, Champignon, Psyllium, Acerola, Pineapple , Banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, mesimacob, cranberry, chondroitin sulfate, zinc, iron, ceramide, silk peptide, glycine, niacin, chesttree, L-cysteine, red wine leaf, millet, hose Tail, biotin, centella asiatica, lotus cup, pynogenol, burdock, rhubarb, clove, rosemary, catechin, puer, citric acid, brewer's yeast, merirot, black zinger, ginger, garlic, nattokinase, beige Koji, Tocotrienol, Lactoferrin, Cinnamon, Buckwheat Buckwheat, Cocoa, Yuzu Seed Extract, Perilla Seed Extract, Lychee Seed Extract, Evening Primrose Extract, Black Rice Extract, α-Lipoic Acid, Gabba, Raw Coffee Bean Extract, Buffalo Extract, Kiwi Seed Extract, Wenzhou mandarin orange extract, red ginger extract, astaxanthin, walnut extract, leek seed extract, red rice extract, kanka extract, white jellyfish polysaccharide, fucoxanthin, lingonberry extract, cherry blossom extract, maquiberry extract, Japanese cherry extract, rice polyamine, wheat Polyamine) and the like can also be blended.

  As a specific production method, an energy metabolism activator or the like is spray-dried or freeze-dried together with powdered cellulose, and this is easily made into a powder, granule, tablet, or solution so as to be easily contained in a food or drink (instant food, etc.). be able to. In addition, an energy metabolism activator or the like can be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof to form a liquid and added to a beverage or added to a solid food. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules and added to a beverage or a solid food.

  As the addition amount when the energy metabolism activator of the present invention is applied to foods and drinks, the main purpose is to maintain health, so the total content of active ingredients is 1 to 20 wt% with respect to the food and drinks. Is preferred.

  The energy metabolism activator or the like of the present invention may be used as a raw material for drugs (including drugs and quasi drugs). It can be produced by appropriately blending the energy metabolism activator of the present invention with a raw material for a pharmaceutical preparation. Examples of pharmaceutical raw materials that can be blended in the energy metabolism activator of the present invention include, for example, excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hydrogenated vegetable oil, kaolin , Talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar) Sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers ( Quaternary ammonium base, sodium lauryl sulfate Helium, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

The administration method of the energy metabolism activator and the like according to the present invention can generally be administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids and the like. However, parenteral administration may also be used. When administered as a parenteral agent, it can be applied in the form of a solution, or in the form of a haptic agent, lotion agent, ointment, tincture, cream, etc. with the addition of a dispersant, suspension, stabilizer, etc. can do.
The dosage may vary depending on the administration method, medical condition, age of patient, etc., but for adults, it can be generally administered as 0.5 to 1000 mg as an active ingredient per day, and for children, about 0.5 to 500 mg can be usually administered.

Hereinafter, the present invention will be described based on examples.
Example: Preparation of black ginger extract Black ginger was sliced and dried to obtain 100 kg of a dried product. 10 kg of this dried product was crushed and extracted at 80 ° C. with hydrous ethanol having an ethanol concentration of 70 wt% for 2 hours, and the ethanol extract was dried to obtain 3.25 kg of black ginger extract.
The components contained in the black ginger extract were analyzed by HPLC. As a result, it was found that the content of 5,7-dimethoxyflavone was 8 wt% or more and the total flavonoid was 35 wt% or more.

Test Example 1: Evaluation of glucose transporter GLUT4 gene expression promoting effect Mouse myoblast cell line C2C12 (cultured in D-MEM medium containing 10% FCS) was seeded on a 24-well plate (1 × 10 ⁴ cell / ml) and cultured for 24 hours did. Thereafter, the medium was replaced with a medium for differentiation induction (D-MEM medium containing 1% FCS), and black ginger extract (5,7-dimethoxyflavone content: 10.4%, total flavonoid content: 36.7%) was added to a final concentration of 0.1, It was added to a concentration of 1,3,10 μg / ml (DMSO concentration 0.1% (v / v)) and cultured for 1 week. For control, DMSO was added at a concentration of 0.1% (v / v) to the medium. After culturing for 1 week, RNA was extracted from the cells. The mRNA expression level of the glucose transporter GLUT4 was quantified for the obtained RNA using a quantitative RT-PCR method. At this time, GAPDH was used as an endogenous control. The result is shown in FIG.

Results and Effects of Examples in Test Example 1 As shown in FIG. 1, as a result of quantifying the expression of the sugar transporter GLUT4 in a mouse myoblast cell line (C2C12) added with black ginger extract and cultured for one week, The expression level was significantly increased by adding the extract.

Test Example 2: Evaluation of Lactate Uptake Transporter Gene Expression Promotion Action Mouse myoblast cell line C2C12 (cultured in FCS10% -containing D-MEM medium) was seeded on a 24-well plate (1 × 10 ⁴ cell / ml) and cultured for 24 hours did. Thereafter, the medium was changed to a medium for differentiation induction (D-MEM medium containing 1% FCS), and black ginger extract (5,7-dimethoxyflavone content: 10.4%, total flavonoid content: 36.7%) was added to a final concentration of 0.1. 1,3,10 μg / ml (DMSO concentration 0.1% (v / v)), and cultured for 1 week. For control, DMSO was added at a concentration of 0.1% (v / v) to the medium. After culturing for 1 week, the cells were collected, and RNA was extracted from the cells. The mRNA expression level of the lactic acid uptake transporter MCT1 was quantified from the obtained RNA using a quantitative RT-PCR method. At this time, GAPDH was used as an endogenous control. The result is shown in FIG.

As shown in FIG. 2, as a result of quantifying the expression of the monocarbaic acid transporter (lactate transporter) MCT1 in mouse myoblast cell line C2C12 cultured for one week with black ginger added, the expression level was increased by adding black ginger extract. An increase was observed.

Test Example 3: Evaluation of glucose uptake action in muscle cells Mouse myoblast cell line C2C12 (cultured in D-MEM medium containing 10% FCS) was seeded on a 48-well plate (1 × 10 ⁴ cell / ml) and cultured for 24 hours. . Thereafter, the medium was replaced with a medium for differentiation induction (D-MEM medium containing 1% FCS), and black ginger extract (5,7-dimethoxyflavone content: 10.4%, total flavonoid content: 36.7%) was added to a final concentration of 0.1, It was added to a concentration of 1,3,10 μg / ml (DMSO concentration 0.1% (v / v)) and cultured for 1 week. For control, DMSO was added at a concentration of 0.1% (v / v) to the medium. To the cells cultured for 1 week, 2-deoxyglucose (2-DG) was added to a final concentration of 1 mM and cultured for 20 minutes. After culturing, each well was washed with PBS, and 10 mM Tris-HCl (pH 8.0) or RIPA buffer was added to collect cells. Cells collected with 10 mM Tris-HCl (pH 8.0) were examined for the amount of sugar using 2-Deoxyglucose (2DG) Uptake Measurement Kit (Cosmo Bio Inc., OKP-PMG-K01) (Result a). On the other hand, the cells collected with RIPA buffer were left on ice for 15 minutes, and then the supernatant was collected by centrifugation, and the total protein amount was examined by BCA method (result b). For the sugar uptake ability, a value obtained by correcting the amount of sugar with the total protein amount (result c = result a / result b) was used to calculate the influence of the black ginger extract on sugar uptake. The result is shown in FIG.

Results and Effects of Examples in Test Example 3 As shown in FIG. 3, the amount of 2DG uptake for 20 minutes was measured using Kit, and as a result, the addition of black ginger extract significantly reduced the concentration from 0.1 μg / ml. It was found that sugar uptake was activated. Therefore, it was confirmed that the black ginger extract has an action of activating the sugar uptake ability through increased expression of the sugar transporter GLUT4. Therefore, it was confirmed that the black ginger extract can be used as an excellent sugar uptake promoter in muscle cells.

Test Example 4: Evaluation of lactate uptake action in muscle cells Mouse myoblast cell line C2C12 (cultured in D-MEM medium containing FCS 10%) was seeded on a 96-well plate (1 × 10 ⁴ cell / ml) and cultured for 24 hours. . Thereafter, the medium was replaced with a medium for differentiation induction (D-MEM medium containing 1% FCS), and black ginger extract (5,7-dimethoxyflavone content: 10.4%, total flavonoid content: 36.7%) was added to a final concentration of 0.1, It was added to a concentration of 1,3,10 μg / ml (DMSO concentration 0.1% (v / v)) and cultured for 1 week. For control, DMSO was added at a concentration of 0.1% (v / v) to the medium.
For the cells cultured for 1 week, each well was washed with PBS, a new medium was added, and the cells were cultured for 3 hours. After 3 hours, the medium was collected and each well was washed with PBS. Next, the buffer or RIPA buffer in L-Lactate Assay Kit (Colorimetric) (Abcam, ab65331) was added to recover the cells. The amount of lactic acid in the cells and medium collected with the buffer in the kit was examined using the kit (result a). On the other hand, the cells collected with RIPA buffer were left on ice for 15 minutes, and then the supernatant was collected by centrifugation, and the total protein amount was examined by BCA method (result b). For the sugar uptake ability, a value obtained by correcting the amount of lactic acid with the total protein amount (result c = result a / result b) was used to calculate the influence of the black ginger extract on lactic acid uptake. The result is shown in FIG. In FIG. 4, A is a graph showing the amount of lactic acid in muscle cells, and B is a graph showing the amount of lactic acid in the medium.

Results and Effects of Examples in Test Example 4 As shown in FIG. 4, the amount of lactic acid in the cells and in the medium was quantified using Kit, and as a result, the amount of lactic acid in the cells (uptake of lactic acid) was significant. Although it was not a difference, an increasing trend was observed (FIG. 4A). This suggests that lactic acid taken up into cells was rapidly metabolized and converted into energy. On the other hand, the amount of lactic acid in the medium decreased in a concentration-dependent manner (FIG. 4B). Thereby, it was confirmed that the lactic acid in a culture medium was taken up actively. Therefore, it was confirmed that the black ginger extract can be converted into energy by increasing the amount of lactic acid taken up through the increased expression of the lactic acid transporter MCT1, and further rapidly metabolizing the taken-in lactic acid. From the above, it was confirmed that the black ginger extract can be used as a lactic acid uptake promoter in muscle cells and an energy metabolism activator in muscle cells.

From the above results, the myoblasts (Myotube) induced to differentiate by black ginger extract are (1) activated sugar uptake, and (2) actively incorporated and metabolized lactic acid. It was confirmed that As a result, it was found that black ginger extract can produce high-quality muscles without increasing the muscle cells themselves.

Although the compounding example of the energy metabolism activator etc. (black ginger extract) of this invention is given to the following, the following compounding example does not limit this invention.
Formulation Example 1: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Black ginger extract 0.5
100.0wt%

Formulation Example 2: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Kiwi juice 4.0
Kiwi flavor 0.6
Dye 0.02
Black ginger extract 1.0
100.0wt%

Formulation Example 3: Candy
50.0 wt% sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Black ginger extract 0.4
100.0wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Black ginger extract 0.4
Perfume
Water residue
100.0wt%

Formulation Example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Black ginger extract 0.05
Perfume
Purified water residue
100.0wt%

Formulation Example 6: Soft capsule
Rice germ oil 87.0wt%
Emulsifier 12.0
Black ginger extract 1.0
100.0wt%

Formulation Example 7: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Black ginger extract 1.0
100.0wt%

Formulation Example 8: Oral granules (pharmaceuticals)
Black ginger extract 1.0wt%
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 8.0
Polyvinylpyrrolidone 1.0
100.0wt%

Formulation Example 9: Tablet
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Black ginger extract 0.5
Purified water 3.9
100.0wt%

Formulation Example 15: Cat food
Corn 34.0wt%
Flour 35.0
Meat meal 15.0
Beef tallow 8.9
Salt 1.0
Skipjack extract 4.0
Black ginger extract 1.0
Taurine 0.1
Vitamins 0.5
Minerals 0.5
100.0wt%

Formulation Example 16: Dog food
Corn 30.0wt%
Meat (chicken) 15.0
Defatted soybean 10.0
Flour 25.0
Mosses 5.0
Black ginger extract 5.0
Animal fats and oils 8.9
Oligosaccharide 0.1
Vitamin 0.5
Mineral 0.5
100.0wt%

  As described above, the present invention can provide an energy metabolism activator for muscle cells that is safe and has few side effects by using black ginger extract.

Claims (5)

A sugar transporter gene expression promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient. Lactate transporter gene expression promoter in muscle cells containing black ginger and / or its extract as an active ingredient. A sugar uptake promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient. A lactic acid uptake promoter in muscle cells containing black ginger and / or an extract thereof as an active ingredient. An energy metabolism activator in muscle cells comprising black ginger and / or an extract thereof as an active ingredient.