JP2004315512A - Functional composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a functional composition enforcing immunological competence, preparing homeostasis balance, making a constitution resistant to diseases and recovering from diseases, expressing beautifying effects such as water retention of skin, improvement in skin water content, and the like. <P>SOLUTION: This functional composition contains a component obtained from Sparassis crispa and one or more kinds selected from the following (a) group or those selected from (b) group: (a) Agaricus blazei, Grifola frondosa, Polyporus umbellatus, pentinus edodes, Bracket fungus of the genus fomes, Pleurotus cytidiosus, Inonotus obliqus, Coriolus versicolor, Pleurotus cornucopiae var. citrinopileatus, Phellinus linteus, L.runda, Elfvingia applanata, Asafetida bamboo, Hericium erinaceum, Crdyceps spp., Cordyceps militaris, Matsutake mushroom, Lyophyllum decastes, isoflavone aglycones, isoflavones, (b) ceramide, chollagen, hyaluronic acid, β-carotene, polyphenols, acerola, vitamin C, ocean deep sea water and camu camu. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention strengthens immunity and adjusts its homeostasis balance, creating a constitution that is unlikely to become ill, and a constitution that recovers from illness, and also has cosmetic effects such as restoring elasticity of the skin, water retention of the skin, and improving moisture content It relates to a functional composition to exert.

  We are originally exposed to the invasion of viruses and microorganisms from the outside world, and we live in a situation where foreign self-substances (for example, cancer, self-dead cells, etc.) appear inside. They can survive even under such conditions because they have a mechanism (immunity) to eliminate such foreign substances and defend themselves. Generally, stress, infectious diseases (measles, influenza, AIDS, etc.), chemical exposure, etc. are considered to be factors that lead to a decrease in immunity, and they decrease with age. Therefore, such a decrease or abnormality in the body defense ability induces various unfavorable diseases, and there are concerns about non-healthy symptoms such as loss of appetite, insomnia, stiff shoulders, headache, and skin elasticity.

On the other hand, Hanabiratake used in the present invention is a very small mushroom that grows on larch. It is an edible mushroom that is chewy and features a pure white color and a shape like leaf peony. Up until now, it has been said that this bamboo shoot has slow growth and artificial cultivation is very difficult. Recently, however, a new cultivation method that can be cultivated in a relatively short period of time has been established (for example, Patent Document 1 and Patents). This is a mushroom that can be supplied on a commercial scale. Yukuzen et al. Focused on extracts of fruit bodies of Hanabiratake, and proposed proposals on extracts of Hanabiratake fungus useful for various bioactive compositions and pharmaceuticals, bioactive compositions containing them as active ingredients, and antitumor agents (For example, refer to Patent Document 3 and Patent Document 4). Furthermore, Ohno et al. Have reported on the antitumor action of β-glucan derived from Hanabiratake (Non-patent Document 1).
reference).

Among them, it has been shown that MH-3 Hanabiratake selected by artificial cultivation has an immunostimulatory effect (see Patent Document 4).
Japanese Patent Laid-Open No. 11-56098 JP 2002-125460 A JP 2000-217543 A JP 2002-125460 A Biol. Pharm. Bull., Pharmaceutical Society of Japan, 23 (7) 866 (2000)

  However, the above-described immunostimulatory action is not yet sufficient and does not have a cosmetic effect.

  The present invention is intended for beauty such as strengthening self-immunity function and adjusting its homeostasis, creating a constitution that is unlikely to cause illness, maintaining health and water retention, and improving moisture content. An object is to provide a useful functional composition.

  The present inventors have found that the above problem can be solved by mixing a component obtained from a specific material into a component obtained from Hanabira bamboo, and have completed the present invention.

  That is, the present invention contains a component obtained from Hanabira bamboo, one or more components selected from the following group (a) and / or one or more components selected from group (b): The gist of the functional composition is characterized by the above.

(A) Agaricus, Maitake, Choreimaitake, Shiitake, Reishi, Ohiratake, Chaga, Kawaratake, Tamogitake, Mishima Kobu, Murasakishiji, Kofukisarokoshitake, Ahitake, Yamabushitake, Cordyceps, Hoshibongo, Tadpole b) Ceramide, collagen, hyaluronic acid, β-carotene, polyphenol, acerola, vitamin C, deep sea water, cam cam Another gist of the present invention is a functional food mainly composed of the above functional composition. Is.

  The functional composition of the present invention enhances immunity and adjusts its homeostasis balance, creating a constitution that is unlikely to cause illness, a constitution that recovers from illness, recovery of skin elasticity, water retention of skin, moisture content A cosmetic effect such as improvement can be exhibited.

  Hereinafter, the present invention will be described in detail.

  Functionality in the present invention means strengthening immunity and adjusting its homeostasis balance, a constitution that is difficult to get sick, an immunostimulatory action that is necessary to create a constitution that recovers from a disease, water retention power of skin, moisture It also refers to an action that also exhibits a beauty effect on human skin such as rate improvement.

  Here, as an index for the effect on human skin, there are moisturizing skin and improving rough skin.

  With respect to skin moisturization, the effect can be known using a conventionally known measurement method such as measurement of transepidermal water loss (TEWL), measurement of keratin water content, and the like. For the measurement of TEWL, for example, an Evaporimeter (ServoMed, Sweden), Tewameter (Courage + Khazaka, Germany) or the like can be used. In addition, for example, Corneometer (Courage + Khazaka Germany), Skikon-200 (IBS Co., Ltd.) or the like can be used for the measurement of the amount of horny moisture. About the improvement of rough skin, the effect can be known by visual observation and the sense of the person concerned.

  Ingredients obtained from Hanabiratake used in the present invention are as follows: Hanabiratake fruit body, dried product thereof, pulverized product thereof, Hanabiratake fruit body extract, Hanabiratake mycelium, dried product thereof, and pulverized product thereof Or any one of the extracts obtained from the mycelium of Hanabiratake or a combination of two or more thereof.

  The fruit body of the flower is a natural one or may be obtained by artificial cultivation. Artificial cultivation can be performed by creating a fungus bed for artificial cultivation (see, for example, JP-A Nos. 11-56098, 2002-369621, and 2002-125460).

  In addition, the method for obtaining mycelium by cultivating Hanabiratake may be either a solid culture method or a liquid culture method usually used for culture of basidiomycetes, but the latter method is preferably used from the viewpoint of productivity. The culture medium only needs to contain various nutrients necessary for the growth of bacteria, and may be a normal medium formulation. That is, as the carbon source, any carbon source that can be assimilated such as glucose, sucrose, maltose, and starch can be used. As a nitrogen source, for example, ammonium sulfate salt, ammonium nitrate salt, urea, etc., as a natural complex nutrient source, for example, potato extract, carrot extract, malt extract, peptone, koji extract, yeast extract, yeast powder, etc. can be used, Other trace element inorganic salts, vitamins and the like necessary for growth may be added as appropriate.

  Cultivation is usually performed under aerobic conditions. For example, a shaking culture method or an aeration and agitation culture method is used. The agitation during the culture may be performed by reciprocal shaking or rotational shaking for several minutes every 24 hours, but may be continuously shaken. The culture temperature is preferably 15 ° C to 40 ° C, more preferably around 20 ° C to 30 ° C. The pH during the culture is preferably adjusted to a range of 3.0 to 9.0, more preferably 4.5 to 7.0. Moreover, although it is preferable not to illuminate during culture | cultivation, the illumination for about 11 to 14 hours a day is possible.

  The number of days of culture varies depending on the culture conditions such as the physical environment and the culture composition, but it is sufficient that the mycelium grows. Usually, the maximum mycelium is produced in 2 to 120 days, particularly preferably 5 to 90 days. The time is good.

  After completion of the culture, the mycelium and the culture filtrate are separated by centrifuging or filtering the culture liquid. Centrifugation can be performed by a centrifugal operation that gives a gravitational acceleration of 100 to 5000 G, preferably 800 to 3000 G. The filtration is performed using a membrane filter of 3.5 to 200 mesh, particularly preferably 4 to 16 mesh.

  As a method for drying the fruit body or mycelium of Hanabiratake obtained as described above, hot air drying, vacuum freeze drying, cold air drying, far-infrared drying and the like can be used, and hot air drying is desirable. Although dried Hanabira bamboo can be used as it is as the functional composition of the present invention, it is usually preferable to use a pulverized product or powder. As a method for pulverizing Hanabiratake, a stone pulverization method, a ball mill method, a collision pulverization method such as a jet mill, or the like can be used.

  In addition to the above, components obtained from the fruit body or mycelium of the moss, which are used in the present invention, are also effective (extracts, etc.) that are extracted from the fruit body or mycelium as it is or after being dried.

  There are no particular restrictions on the method used to obtain the extract from the fruit bodies or mycelia of the moss, but in addition to solvent extraction, supercritical extraction, physical extraction using a ball mill or blender, and an enzyme to increase extraction efficiency. The method of adding etc. can be combined suitably. As the solvent used for solvent extraction, an aqueous solvent or a non-aqueous solvent can be used. Specific examples of aqueous solvents include water itself, alkaline water to which alkali or other basic substances are added, aqueous solvents to which organic solvents compatible with alcohol, etc. are added, acidic water to which acids or acidic substances are added, organic An aqueous solution containing an acid (for example, lactic acid, acetic acid, etc.), an inorganic salt (for example, yeast food), or a mixture of two or more of these can be suitably used.

  Specific examples of non-aqueous solvents include ethyl alcohol, isopropyl alcohol, glycerin, propylene glycol, dimethyl sulfoxide, and dimethylformamide.

  Solvent extraction can be performed by the following operations. That is, 2 to 10 parts by mass of a solvent is added to 1 part by mass of freshly crushed or sliced fresh fruit or dried body thereof, preferably 40 to 120 ° C., more preferably about 80 ° C. for 2 hours or more or 90 ° C. For 30 minutes or longer. Thereafter, solid-liquid separation is performed, and the extract can be further concentrated.

  This solvent extraction process may be performed in multiple stages, or may be performed as a multistage process using different solvents. The amount of the solvent used is not particularly limited, but is preferably determined in consideration of the weight of the target bamboo shoot, the weight of the solvent or residue, and the post-treatment burden.

  It is desirable that the functional composition of the present invention is prepared so that components obtained from the algal bloom obtained by the method described above can be ingested in an amount of 50 mg to 10 g, preferably 100 mg to 3 g per day in terms of solid content.

  Group (a) contained in the present invention together with agaricus mushrooms: Agaricus, Maitake, Choreimaitake, Shiitake, Ganoderma, Ohitake, Chaga, Kawaratake, Tamogitake, Meshimabubu, Murasakixiji, Kofukisarokoshitake, Agittake, Yamabushitake, Nematode Matsutake, Hatake-shimeji, isoflavone aglycone, and isoflavone have a function of further enhancing the immunity of Hanabiratake.

  As these components, known ones can be used, and those commercially available or collected from the natural world may be included raw or as a dried product, and from these, by the same method as described above. The extracted extract may be included. Moreover, you may perform extraction operation simultaneously, after mixing with Hanabira bamboo.

  The component (a) should be contained in an amount in the range of 1/10 to 2 times, preferably 1/2 to 1.5 times the amount of the active ingredient of Hanabira bamboo.

  In addition, group (b) contained together with Hanabiratake: Ceramide, Collagen, Hyaluronic Acid, β-Carotene, Polyphenol, Acerola, Vitamin C, Deep Sea Water, Camcam, Propolis, Royal Jelly has a cosmetic effect in accordance with the immunity of Hanabiratake It is a component that demonstrates it. As these components, known components can be used, and those that are commercially available, collected from nature, or separated and purified by a known method are effectively used.

  The ceramide of group (b) used in the present invention includes sugar ceramide (glycosylceramide) belonging to sphingoglycolipid in addition to ceramide belonging to sphingolipid. Those obtained by solvent extraction from raw materials of animal and plant origin can be suitably used, and those extracted from plant origin, especially potatoes, are particularly preferred from the viewpoint of safety and content. The potatoes used as raw materials may be any kind of sweet potato, potato, taro, yam, konnyaku potato, and long yam. Particularly preferred are konjac potatoes, specifically konjac, konjac ginger, konjac. Examples include pulverized and dried koji, that is, konjac coarse powder, konjac fine powder, and konjac powder. Of these, it is particularly preferable to use konjac powder. Konjac powder is a fine powder that scatters around when producing konjac flour from konjac rice and accounts for about 45% of the entire konjac rice cake.

  Solvents used to obtain an extract containing ceramide include water and monohydric alcohols such as ethanol, methanol, isopropanol and normal butanol, polyhydric alcohols such as ethylene glycol, propylene glycol and glycerin, acetone, Other oxygen-containing organic solvents such as methyl ethyl ketone, 1,4-dioxane, diethyl ether, ethyl acetate, tetrahydrofuran and dimethyl sulfoxide, nitrogen-containing organic solvents such as dimethylformamide, pyridine and acetonitrile, and halogen-containing systems such as chloroform and dichloroethane High pressure fluids such as organic solvents, hydrocarbons such as normal hexane and isooctane, supercritical carbon dioxide, supercritical fluoroform, and supercritical ethane. Among these, when the extract is used orally, water, ethanol, acetone, normal hexane, and supercritical carbon dioxide are particularly preferably used. These solvents may be used alone or as a mixture of two or more solvents.

  The ceramide of group (b) used in the present invention may be produced by the above-described method, and commercially available products (for example, manufactured by Unitika Ltd.) can also be suitably used.

  The components of group (b) may be contained in an amount of 1/10 to 5 times, preferably 1/2 to 3 times the amount of the active ingredient of Hanabira bamboo.

  In addition to the essential components, additives can be arbitrarily selected and used in combination with the functional composition of the present invention as necessary. Additives include various vitamins, pantothenic acid, folic acid, biotin, zinc, calcium, magnesium, amino acids, oligosaccharides, propolis, royal jelly, EPA, DHA, coenzyme Q10, chondroitin, lactic acid bacteria, lactoferrin, isoflavones, prunes, chitin, chitosan And functional materials such as glucosamine, and excipients such as reduced maltose and crystalline cellulose, but are not limited thereto.

  The functional composition of the present invention is obtained by processing the composition of the present invention for use in, for example, processed foods, pharmaceuticals, confectionery, beverages, internal preparations (including preparations used for animals), and cosmetics. Alternatively, existing foods, pharmaceuticals, confectionery, beverages, internal preparations (including preparations used for animals) and cosmetics can be used by including the composition of the present invention. As a form of the functional composition of the present invention, ampoules, capsules, pills, tablets, powders, granules, solids, liquids, gels or bubbles, creams, and the like can be arbitrarily used.

  Specifically, beauty / health drink or food (vitamin supplementation, nourishing tonic, recovery from fatigue, improvement of rough skin, improvement of body structure, beauty hair, hair growth / hair growth), pharmaceuticals, confectionery, Japanese confectionery, gum, candy, caramel, etc. General soft drinks such as fruit juices, processed soft drinks such as kamaboko and chikuwa, livestock products such as sausages and ham, raw noodles, boiled noodles, buckwheat noodles, sauces, soy sauce, sauce, sugar, Seasonings such as honey, powdered candy, syrup, etc., spices such as curry powder, mustard powder, pepper powder, dairy products such as jam, marmalade, chocolate spread, cheese, butter, yogurt, beauty / health cream, beauty / health liquid Products, beauty / health fopplies, etc.

  These can be produced by a conventionally known method.

Hereinafter, specific examples will be described.
Example 1
Hanabira bamboo ginger (manufactured by Unitika) was subjected to blanching treatment (sterilization treatment) at 95 ° C. for 3 minutes, followed by drying and pulverization treatment at 35 to 65 ° C. for 18 hours to obtain dried Hanabira bamboo. 150 mg of Reishi mushroom, 150 mg of Chaga, and 70 mg of crystalline cellulose as an excipient were added to 150 mg of the dried dried bamboo shoot, to obtain a tablet (250 mg) of the functional composition of the present invention containing Hanabitake, Reishi, and Chaga. It was.

Example 2
Function of the present invention containing Hanabiratake and ceramide by adding 20 mg of ceramide (3% powder, manufactured by Unitika Co., Ltd.) and 80 mg of crystalline cellulose as an excipient to 150 mg of dried dried leaflet obtained in the same manner as in Example 1. A tablet (250 mg) of the composition was obtained.

Example 3
To 150 mg dried Hanabira bamboo obtained in the same manner as in Example 1, 20 mg reishi, 10 mg chaga, 20 mg ceramide (3% powder, manufactured by Unitika) and 50 mg crystalline cellulose as an excipient were added. A tablet (250 mg) of the functional composition of the present invention containing turf, chaga and ceramide was obtained.

Example 4
20 mg of ceramide (3% powder, manufactured by Unitika), 10 mg of hyaluronic acid, and 70 mg of crystalline cellulose as an excipient were added to 150 mg of dried dried lobster obtained in the same manner as in Example 1. The tablet (250 mg) of the functional composition of this invention containing this was obtained.

Comparative Example 1
Tablets (250 mg) were prepared using only crystalline cellulose used as an excipient.

Test Example 1 [Anti-tumor test using mice]
For the powders having the same composition as the tablets obtained in Examples 1 to 4 and Comparative Example 1, antitumor tests using mice were performed as follows.

  A mouse was inoculated with a sarcoma 180 solid subcutaneous tumor, and the powder of the present invention was orally administered for 10 days from the fifth day after ingestion, and the subsequent tumor growth inhibition rate was measured. The dose was converted to the dried habana teke obtained in Example 1 and the amount corresponding to 10 mg / kg body weight per day.

  The obtained results are shown in Table 1. As is clear from Table 1, the functional composition of the present invention had a tumor growth inhibitory action.

Test Example 2 [Effect test on human skin]
About the tablet obtained in Examples 1-4 and the comparative example 1, the effect test to human skin was done as follows.

A total of 69 volunteers, 35 males and 34 females, were recruited. These subjects were inoculated with the functional composition of the present invention for 1 month, and the following items were measured before and after the inoculation. The inoculation amount was 2 tablets per day.
(1) Measuring method of TEWL The TEWL was measured by using a Tewameter (Courage + Khazaka Germany) and measuring a site 1 cm below the right eye and 1 cm below each time.
(2) Measuring method of keratin water content Corneometer CM825 (Courage + Khazaka Germany) was used to measure the keratin water content, and the site 1 cm below the right eye was measured every time. The measurement was performed 10 times per person, and the average value was used as the data for each person.
(3) Self-evaluation regarding rough skin and beautiful skin effect A questionnaire was conducted on the subjects before and after the start of the test, and the degree of improvement regarding rough skin and beautiful skin effect of each person was evaluated based on the following criteria.
5: considerably improved, 4: slightly improved, 3: no change, 2: slightly deteriorated, 1: considerably deteriorated Table 2 shows the results obtained. As is clear from Table 2, the functional composition of the present invention had an effect on human skin.

Claims (2)

It contains one or more components selected from the following components (a) and / or two or more components selected from group (a), and / or one or two or more components selected from group (b) Functional composition.
(A) Agaricus, Maitake, Choreimaitake, Shiitake, Reishi, Ohiratake, Chaga, Kawaratake, Tamogitake, Mishima Kobu, Murasakishiji, Kofukisarokoshitake, Ahitake, Yamabushitake, Cordyceps, Hoshibongo, Tadpole b) Ceramide, collagen, hyaluronic acid, β-carotene, polyphenol, acerola, vitamin C, deep ocean water, cam cam The functional food which has as a main component the functional composition of Claim 1