JP4739161B2 - Endurance improver - Google Patents

Description

  The present invention relates to endurance improvers, anti-fatigue agents, muscle strength improvers, motor function improvers, and aging inhibitors for exercise in a broad sense.

  In recent years, with the development of transportation means and the development of information and communication technology, there is a lack of exercise. Lack of exercise is considered to cause a decrease in motor function such as a decrease in endurance and a decrease in muscle strength, and a serious adverse effect on the subsequent QOL.

  Muscle atrophy, endurance, and a decrease in muscle strength associated with aging decrease motor function and cause bedridden. In addition, falls during daily movements caused by muscle atrophy and weakness, and the accompanying fractures are the main cause of bedridden in elderly people.

  Therefore, in order to improve and maintain daily life functions and extend a healthy life span, it is important to maintain or enhance motor functions on a daily basis and to suppress a decrease in motor functions associated with aging.

  It may be appropriate to perform exercise training to improve motor function, but people who cannot get enough exercise time due to their busyness or who get tired immediately after exercising will continue. Many people are unable to exercise. In elderly people, practicing moderate exercise or practicing rehabilitation is used as a means to prevent a decline in motor ability. However, in reality, it may be difficult to maintain motivation or injuries. It is quite difficult and a more effective method is desired.

  As one means for solving these problems, it is conceivable to ingest daily food components having an action function improving action such as endurance and muscle strength and an anti-fatigue action. For example, the present inventors have found an endurance improvement effect by ingesting catechin (Patent Document 1). Examples of other reported components having endurance enhancing effects include hawthorn extract (Patent Document 2), ganoderma component (Patent Document 3), proanthocyanidins and lycopene (Patent Document 4). Examples of the component having anti-fatigue action include coenzyme Q10, a carnitine compound (Patent Document 5), a glutamine peptide (Patent Document 6), and the like.

  In addition, as components that can control exercise ability, for example, aging prevention by cystine and theanine (Patent Document 7), muscle atrophy suppression by fruit polyphenol (Patent Document 8), muscle protein degradation suppression by lycopene (Patent Document 9), carboxyl Inhibition of athletic ability decrease using an organic acid having two or more groups or a salt thereof as an active ingredient (Patent Document 10), an anti-fatigue agent using astaxanthin and / or its ester (Patent Document 11), a muscle atrophy inhibitor using proanthocyanidins, and An athletic performance improver (Patent Literature 12, Patent Literature 13) and the like are disclosed. However, at present, the effects on endurance, muscle strength, energy metabolism decrease and muscle atrophy associated with actual aging have not been studied.

Resveratrol is a polyphenol component contained in grapes and their processed wines, peanuts, etc., and has a rich dietary experience. Resveratrol exhibits actions such as anticancer action (Non-Patent Document 1) and anti-arteriosclerosis action (Non-Patent Document 2), and also reports a life extension action via a molecule called Sir (Non-Patent Document 3). Has been. It is also disclosed that it is useful in the prevention and treatment of hyperlipidemia (Patent Document 14) and the prevention or treatment of cerebral and age-related diseases (Patent Document 15).
On the other hand, red grape leaf extract has been traditionally known for use as a therapeutic agent for venous insufficiency for a long time. A method for improving blood circulation using red grape leaf extract (Patent Document 16) and a method for treating chronic venous insufficiency ( Patent document 17) is disclosed.

However, the effects of resveratrol and grape leaf extract on endurance and other athletic performance, fatigue, and on endurance and energy metabolism associated with aging have never been known. .
JP 2005-89384 A JP-A-8-47381 JP-A-5-123135 Japanese Patent Laying-Open No. 2005-334022 JP-A-2005-97161 JP-A-2005-97162 International Publication No. 2005-123058 Pamphlet JP 2001-89387 A JP 2004-59518 A Japanese Patent Laid-Open No. 10-17585 JP 2006-16409 A JP 2002-338464 A JP 2005-97273 A JP 2001-72583 A JP-T-2002-527389 JP-T-2006-516542 Special table 2003-511476 gazette Science, vol.275, No.5297, pp218-220, 1998 Cardiovasc Drug Rev, vol.22, No.3, pp169-188, 2004 Nature, vol.425, No.6954, pp191-196, 2003

  The present invention provides pharmaceuticals, quasi-drugs, and foods that are highly safe and have excellent endurance-improving action, anti-fatigue action, motor function-improving action, energy metabolism-activating action, muscle strength-improving action, and aging-inhibiting action. About that.

  As a result of searching for an effective component in improving physical function, the present inventors, among natural materials with abundant dietary experience and high safety, resveratrol and grape leaf extract have an effect of improving endurance, Anti-fatigue action, motor function improvement action, energy metabolism activation action, muscular strength improvement action, endurance reduction suppression action due to aging, muscle atrophy suppression action, energy metabolism reduction suppression action and muscular strength reduction suppression action It was found useful as a strength improver, muscle strength improver, motor function improver, anti-fatigue agent, and antiaging agent. In addition, the present inventors have resveratrol and / or grape leaf extract have an endurance reduction inhibitory effect, muscular strength reduction inhibitory effect, energy metabolism reduction inhibitory effect, muscle atrophy inhibitory effect accompanying aging, and an aging inhibitor I found it useful.

That is, the present invention relates to the inventions 1) to 16) below.
1) Endurance improver containing resveratrol and / or grape leaf extract as an active ingredient.
2) An anti-fatigue agent comprising resveratrol and / or grape leaf extract as an active ingredient.
3) A motor function improver comprising resveratrol and / or grape leaf extract as an active ingredient.
4) An energy metabolism activator comprising resveratrol and / or grape leaf extract as an active ingredient.
5) A muscle strength improver comprising resveratrol and / or grape leaf extract as an active ingredient.
6) An aging inhibitor containing resveratrol and / or grape leaf extract as an active ingredient.
7) A food containing resveratrol and / or grape leaf extract, characterized by having an endurance-improving action, and labeled as being used for improving endurance.
8) A food containing resveratrol and / or grape leaf extract, having an anti-fatigue action, and labeled as being used for anti-fatigue.
9) A food containing resveratrol and / or grape leaf extract, characterized by having an effect of improving motor function, and labeled to be used for improving motor function.
10) A food containing resveratrol and / or grape leaf extract, characterized by having an energy metabolism activating action, and labeled as being used for energy metabolism activation.
11) A food containing resveratrol and / or grape leaf extract, characterized by having a muscular strength-improving action, and labeled to be used for improving muscular strength.
12) A food containing resveratrol and / or grape leaf extract, characterized by having an anti-aging effect, and labeled as being used for anti-aging.
13) Use of resveratrol and / or grape leaf extract for the production of endurance enhancers.
14) Use of resveratrol and / or grape leaf extract for the manufacture of anti-fatigue agents.
15) Use of resveratrol and / or grape leaf extract for the production of a motor function enhancer.
16) Use of resveratrol and / or grape leaf extract for the production of an energy metabolism activator.

  The endurance improver, anti-fatigue agent, exercise function improver, energy metabolism activator, muscle strength improver, and anti-aging agent of the present invention are used to improve endurance against a broad sense of exercise including exercise and daily movement and labor, Foods and pharmaceuticals that exhibit fatigue, motor function improvement, energy metabolism activation, and muscle strength improvement effects, and foods and pharmaceuticals that exhibit effects of suppressing endurance reduction, muscle atrophy, energy metabolism reduction, and muscle strength reduction caused by aging It is also useful. These agents of the present invention contain resveratrol and / or grape leaf extract as an active ingredient, have abundant food experience, have few side effects, and are highly safe.

  Resveratrol according to the present invention means 3,4 ′, 5-Trihydroxy-stilbene, and includes trans-resveratrol, cis-resveratrol and mixtures thereof. In the present invention, at least one of these compounds is used. Moreover, the resveratrol of this invention may be a glycoside (Piceid etc.).

  The resveratrol in the present invention is extracted from a natural product containing resveratrol such as grapes, peanuts, peanuts, and tadaceae plants such as pits, in addition to high-purity products synthesized using organic chemistry or microorganisms. Can be used after appropriately purifying. In addition, plants, microorganisms, various food extracts, partially purified products, and processed products containing resveratrol may be used. Moreover, resveratrol isolated from those extracts, partially purified products, and processed products can also be used.

As natural products, grape and itadori are preferable.
Grapes can be classified into European grapes ( Vitis vinifera ) and American grapes ( Vitis labrusca ), either of which may be preferred, but European grapes are preferred.
Specific types of grapes are Delaware, Kyoho, Koshu, Pione, Muscat, Chenam Blanc, Grenache, Mataro, Muller Thurgau, Trebbiano, Berry A, Cabernet Sauvignon, Merlot, Pinot Noir, Cabernet Franc, Schiller, Chardonnay, Sauvignon Blanc, Semillon, Gamay, Riesling, Alligote, Muscadelle, Sauvignon, Forblanc, Muscade, Chenin, Gloro, Pinault Dornis, Pinot Meunier, Gewurztraminer, Pinot Gris, Silvaner, Poolsar, Savanyan, Jacquard , Mondoze, Rouset, Carignan, Sansault, Clerette, Grenache Noir, Mourvedre, Uni Blanc, Nielcio, Skia Carello, Vermancino, Rousanne, Rousanne, Viognier, Boulevard Bran, Macaboo, Mozac, Tana, Colombar, Ran Dove Rel, Malbec, Petit Mansen, Gro Mansen, Petit Verd, Sangiovese, Naviolo, Barbera, Dolce, Carignena, Garnacha , Monastrel, tempranino and the like.

Examples of the extract include an extract from a grape fruit, grape leaf, or a product derived from grape, and in the case of a grape leaf extract, a red grape leaf extract is particularly preferable, and European grape ( Foliae Vitis vinifera L. ) Red grape leaf extract is particularly preferred. Examples of the product derived from grapes include grape juice, wine, juice and residue produced during wine production, juice and wine concentrate.
For extraction, for example, because resveratrol is abundant in grape skins and leaves, a general extraction method that uses water, hot water, alcoholic water, organic solvents, etc., is adopted. it can.
Moreover, since resveratrol is contained also in grape juice, wine, these concentrates, or the solid substance concentrated to dryness, they can also be used. In this case, the grape residue generated during the production can be used as it is, or can be extracted with an organic solvent / water or the like.
As the organic solvent used for the extraction of the resveratrol, alcohol such as ethanol is preferable, and ethanol is particularly preferable.

  In addition, since the Japanese knotweed contains a lot of resveratrol in its roots, it is preferable to use a knotweed extract obtained by the same general extraction method as that described above.

  Resveratrol and / or grape leaf extract according to the present invention, as shown in the examples below, endurance improvement action, anti-fatigue action, muscle strength improvement action, motor function improvement action, energy metabolism activation action, In addition, it has an aging inhibitory action, specifically, an endurance reduction inhibitory action caused by aging, a muscle atrophy inhibitory action, an energy metabolism lowering inhibitory action, and a muscle strength decline inhibitory action. Therefore, resveratrol and / or grape leaf extract is used for endurance improver, anti-fatigue agent, motor function improver, energy metabolism activator, muscle strength improver and aging inhibitor (hereinafter referred to as “endurance improver etc. And can be used to produce these agents. Endurance improvers, etc. are used as drugs for humans or animals, quasi-drugs, and foods that demonstrate endurance, anti-fatigue, muscle strength, motor function, energy metabolism activation, and anti-aging effects. Is possible. In addition, resveratrol and / or grape leaf extract is based on the concept of endurance improvement, anti-fatigue, motor function improvement, energy metabolism activation, muscle strength improvement or aging suppression in those with exercise deficiency, middle-aged and elderly people, bed rest persons, It can be applied to foods that indicate this, functional foods, foods for the sick, and foods for specified health use. Furthermore, it can be used to cope with not only exercise deficient persons, middle-aged and elderly persons, bed rest persons, but also all people including athletes.

  In the present invention, “aging inhibition” refers to inhibiting physiological changes caused by aging. Such physiological changes include decreased endurance, muscle atrophy, decreased energy metabolism, and decreased muscle strength.

  Examples of the dosage form when the endurance improver of the present invention is used as a pharmaceutical product or quasi-drug include oral administration using tablets, capsules, granules, powders, syrups, etc., or injections, suppositories, and inhalants. And parenteral administration by transdermal absorption agents, external preparations and the like. In order to prepare such various dosage forms, the resveratrol and / or grape leaf extract of the present invention alone or other pharmaceutically acceptable excipients, binders, A bulking agent, a disintegrating agent, a surfactant, a lubricant, a dispersing agent, a buffering agent, a preservative, a flavoring agent, a fragrance, a coating agent, a carrier, a diluent, and the like can be used in appropriate combination. Of these dosage forms, the preferred form is oral administration, and when an oral liquid preparation is prepared, it can be produced by a conventional method by adding a flavoring agent, a buffering agent, a stabilizer and the like.

In the case of using the endurance improver of the present invention as food, various foods such as breads, cakes, noodles, confectionery, jelly, frozen foods, ice creams, dairy products, beverages, soups, etc. In addition, the same forms (tablets, capsules, syrups, etc.) as the above-mentioned oral administration preparation are mentioned. Examples of the beverage include fruit juice beverages, carbonated beverages, tea-based beverages, near water, sports beverages, milk beverages, alcoholic beverages, and soft drinks.
To prepare various forms of food, resveratrol and / or grape leaf extract of the present invention alone or other food ingredients, solvents, softeners, oils, emulsifiers, preservatives, fragrances, A combination of stabilizers, colorants, antioxidants, humectants, thickeners, etc., as appropriate, endurance foods, muscle strength foods, motor function foods, anti-fatigue foods, anti-aging products It can be used as food, pet food and the like.

  In addition, the endurance improver of the present invention can be used as a form of a nutritional composition such as an enteral nutrient in elderly people who are difficult to supplement with an appropriate amount of nutrition or in bed rest. .

  The compounding amount of resveratrol and / or grape leaf extract with respect to these varies depending on the use form, but in the form of food, resveratrol is usually 0.0001 to 5% by mass, further 0.001 to It is preferable to set it as 2 mass%, especially 0.002-1 mass%, and a grape leaf extract (dry matter conversion) is usually 0.0001-10 mass%, 0.001-5 mass% especially 0.00. It is preferable to set it as 002-2 mass%. In the case of pharmaceutical preparations other than the above, for example, oral solid preparations such as tablets, granules, capsules, and oral liquid preparations such as oral liquids and syrups, resveratrol is usually 0.01 to 95% by mass, Furthermore, it is preferable to set it as 5-90 mass%, especially 10-50 mass%, and a grape leaf extract (dry matter conversion) is 0.01-95 mass% normally, Furthermore, 5-90 mass%, especially 10-50. It is preferable to set it as the mass%.

  The dose (effective intake) of resveratrol and / or grape leaf extract (in terms of dry matter) of the present invention is preferably 1 to 2000 mg / 60 kg body weight per day. At this time, resveratrol is particularly preferably 3 to 700 mg / 60 kg body weight, more preferably 5 to 500 mg / 60 kg body weight, and most preferably 20 to 400 mg / 60 kg body weight. The grape leaf extract (in terms of dry matter) is particularly preferably 5 to 2000 mg / 60 kg body weight, more preferably 10 to 1000 mg / 60 kg body weight, and most preferably 40 to 800 mg / 60 kg body weight.

  The typical test examples and examples of the present invention are shown below.

Test Example 1 Resveratrol Endurance Improvement / Anti-Fatigue Effect Evaluation of resveratrol endurance improvement / anti-fatigue effect was performed as follows. Resveratrol manufactured by Cayman was used.

  Endurance improvement and anti-fatigue effects were evaluated by a method for measuring the limit swimming time of mice in a running water pool (reference: Am J Physiol Regul Integr Comp Physiol, Vol. 288, R708-R715, 2005). The experimental pool was used with a transparent acrylic pool (length 90 × width 45 × depth 45 cm) filled with water to a depth of 38 cm and maintained at 34 ° C. with a heater. The water flow was created using a pump (C-P60H, manufactured by Hitachi), and the flow rate was adjusted by connecting a water flow meter (Tokyo Flow Meter Laboratory) to the pump and opening and closing the valve. Moreover, the flow velocity was confirmed with a velocimeter (SV-101-25S, Sanko Precision Industry).

  As experimental animals, 5-week-old Balb / c male mice (Charles River) were preliminarily raised for 1 week in a constant breeding environment (23 ± 2 ° C, light period: 7 am to 7 pm). Acclimated. After that, for one week, training was performed three times a week to get used to swimming exercise (30 minutes of swimming exercise was performed on the first day for 30 minutes at a flow rate of 5 L / min, 3 days on the third day, and 6 L / min for 30 minutes on the third day). ). In the next week, with each individual fasted for 2 hours, the limit swimming time at a flow rate of 7 L / min (the time when rescued in a state where the water surface could not be exposed due to breathing was defined as the limit swimming time) was 2. Measured once. The group was divided into two groups, the test food group and the control food group, with 8 animals per group so that there was no difference between the groups in the limit swimming time. Using the diet prepared with the composition shown in Table 1, the animals were reared for 10 weeks. During this time, limit swimming time measurement (7 L / min) and training (6 L / min, 30 minutes) were performed once a week. Table 2 shows the limit swimming time of mice at the 10th week of breeding.

  From Table 2, in the mice that received the test meal containing resveratrol, the marginal swimming time after 10 weeks of breeding was significantly extended compared to the control group, and resveratrol improved endurance, It can be seen that it has an anti-fatigue effect. Moreover, it turns out that it has a motor function improvement effect. From the above, resveratrol is useful as an endurance improver, anti-fatigue agent, and motor function improver.

Test Example 2 Endurance Improvement / Anti-fatigue Effect of Grape Leaf Extract Evaluation on the endurance improvement / anti-fatigue effect of the grape leaf extract and grape seed extract was performed as follows. In addition, the grape leaf extract used the red grape leaf dry extract of Ask Pharmaceutical Co., Ltd., and the grape seed extract used Grape Seed Extract (95%) of Organic Herb.
The same procedure as in Test Example 1 was performed except for the diet prepared with the formulation shown in Table 3. Table 4 shows the limit swimming time of mice in the 10th week of breeding.

  From Table 4, in the mice that received the test meal containing red grape leaf extract, the marginal swimming time after 10 weeks of breeding was significantly extended compared to the control diet group, and the grape leaf extract was endurance. It can be seen that it has an improving effect and an anti-fatigue effect. Moreover, it turns out that it has a motor function improvement effect. On the other hand, in the group that ingested the grape seed extract diet, the marginal swimming time tended to be extended as compared with the control diet group, but the extent of extension was small compared with the grape leaf extract diet group, which was significant. It was not a big difference. The grape leaf extract and grape seed extract both contain polyphenols and anthocyanins. From the results of this test, the composition of the components contained in the grape leaf extract is an endurance improver and anti-fatigue agent. Therefore, it is considered more useful as a motor function improver.

Test Example 3 Resveratrol Aging Inhibition Effect Resveratrol was evaluated for aging as follows. Resveratrol was manufactured by Organic Herb.

13 weeks old SAM-P1 male mice (aging-promoting model mice) and SAM-R1 male mice (usually aging mice) were preliminarily raised for 5 weeks in individual breeding, then acclimated to running on a treadmill and the limit running time was measured did. Measurements were started after the mice were rested in a treadmill and accustomed to the environment. The belt speed starts at 10m / min, 10, 15m / min for 5 minutes each, 20m / min for 60 minutes, 22m / min for 60 minutes, 24m / min for 60 minutes, 26m / min for 60 minutes, and so on Was performed at a speed of 28 m / min. The time when the mouse could not run on the treadmill was defined as the limit running time, and the exercise endurance was evaluated. The SAM-R1 control group, the SAM-P1 control group, and the SAM-P1 resveratrol group were divided into groups (5 to 8 animals) so that there was no difference in the limit running time of each system.
It was reared for 10 weeks using the diet prepared with the formulation shown in Table 5. Each mouse was subjected to exercise for 30 minutes at 15 m / min three times a week.

  The limit running time of each group was measured after rearing for 8 weeks. Table 6 shows the mouse limit travel time at this time. In addition, after consumption for 9 weeks, oxygen consumption of each group was measured by breath analysis as energy metabolism at rest. For analysis, an 8 chamber chamber of the Oxymax system (Columbus) was used. After the mouse was placed in the chamber and allowed to rest for 6 hours, measurements were taken continuously for 2 minutes every 18 minutes for 24 hours. The average oxygen consumption for 24 hours is shown in Table 7. On the final day of the experiment, the mice were dissected and the gastrocnemius, soleus and plantar muscles were removed, and then the weight was measured to determine the total muscle weight. Table 8 shows the total muscle weight per body weight.

  From the results of Table 6, the limit running time of the SAM-P1 control group, which is an aging-promoting mouse, is significantly reduced compared to the SAM-R1 control group, which is usually an aging mouse, and the endurance decreases with aging. Moreover, the limit running time of the mouse | mouth which ingested the feed which mix | blended the resveratrol was significantly long with respect to the SAM-P1 control group, and it turns out that resveratrol has the endurance fall inhibitory effect by aging. Moreover, it turns out that it has a motor function improvement effect | action.

  From the results shown in Table 7, the oxygen consumption of the SAM-P1 control group, which is a model mouse for aging, is significantly reduced compared to the SAM-R1 control group, which is a normal mouse, and the energy metabolism decreases with age. However, the amount of oxygen consumed by mice ingesting a diet containing resveratrol was significantly higher than that of the SAM-P1 control group, and it can be seen that resveratrol has an effect of suppressing the decrease in energy metabolism due to aging.

From the results in Table 8, the muscle weight in the body weight of the SAM-P1 control group, which is an aging-promoting model mouse, is significantly reduced compared to the SAM-R1 control group, which is usually an aging mouse. I understand that Moreover, it turns out that the muscle weight of the mouse | mouth which ingested the feed which mix | blended resveratrol was significantly larger than the SAM-P1 control group.
From the above, the resveratrol of the present invention is useful as an aging inhibitor, specifically, an endurance reduction inhibitor accompanying aging, a muscle atrophy inhibitor, and an energy metabolism reduction inhibitor.

Test Example 4 Resveratrol aging effect of muscular strength reduction due to aging Resveratrol was evaluated for muscular strength reduction due to aging as follows. Resveratrol was manufactured by Organic Herb.

  A 13-week-old SAM-P1 male mouse (an aging-promoting model mouse) and a SAM-R1 male mouse (usually an aging mouse) were preliminarily raised for 5 weeks in individual breeding, and then acclimated to running on a treadmill. Then, it divided into the SAM-R1 control group, the SAM-P1 control group, the SAM-P1 exercise group, and the SAM-P1 resveratrol group / exercise (each group 5 animals). It was reared for 13 weeks using a diet prepared with the formulation shown in Table 9. Each mouse in the exercise group was subjected to exercise at 15 m / min for 30 minutes three times a week.

  The mice were subjected to dissection after 13 weeks of breeding, and the excised longus extensor muscles were placed in a Krebs solution at 37 ° C. (aeration conditions: 95% -oxygen, 5% -carbon dioxide) with a transducer (FORT100: World Precision Instruments, Inc.). )). Thereafter, electrical stimulation (0.5 ms, 140 Hz) was applied, and the maximum muscle strength was measured. Table 10 shows the measurement results of muscle strength.

From the results of Table 10, the maximum muscle strength of the long-knee extensor muscle of the SAM-P1 control group, which is an aging-promoting mouse, is significantly lower than that of the SAM-R1 control group, which is usually an aging mouse. However, the maximum muscular strength of mice ingesting a diet containing resveratrol is significantly higher than that of the SAM-P1 control group, and it can be seen that resveratrol has an inhibitory effect on muscular strength reduction due to aging.
From the above, the resveratrol of the present invention is useful as an aging inhibitor, specifically, a muscular strength decrease inhibitor accompanying aging.

Formulation Examples Each formulation (1) to (14) for improving endurance, anti-fatigue, muscular strength, improving motor function, activating energy metabolism, improving muscular strength, and suppressing aging was produced.
(1) Fruit juice beverage Fruit juice beverage was produced with the formulation shown in Table 11.

(2) Capsule The composition (300 mg) shown in Table 12 was enclosed in a capsule to produce a capsule.

(3) Tablets The composition shown in Table 13 (1 tablet = 250 mg) was tableted to produce tablets.

(4) Granule The composition shown in Table 14 (1 bag = 500 mg) was mixed to produce a granule.

(5) Food The composition shown in Table 15 (1 tablet = 1000 mg) was tableted to produce a chewable tablet food.

(6) Beverage The composition shown in Table 16 was mixed to produce a beverage.

(7) Capsule The composition shown in Table 17 (300 mg) was encapsulated in a capsule to produce a capsule.

(8) Jelly food The composition shown in Table 18 was dissolved at 65 ° C., held at 85 ° C. for 5 minutes, sterilized, and dispensed into a 100 ml container. The jelly food containing resveratrol of the present invention was prepared by allowing to stand for 8 hours and cooling to 5 ° C. while gradually cooling.

(9) Enteral nutrient for inhibiting aging With the formulation shown in Table 19, the enteral nutrient for inhibiting aging of the present invention was encapsulated in a retort pouch according to a conventional method, sterilized and prepared. Minerals are organic or inorganic salt mixtures such as Na, K, Ca, Mg, P, C1, and Fe. Vitamins are vitamins A, D, E, vitamins B1, B2, B6, B12, C, niacin, pantothene. A mixture of acids, etc. was used in an amount consistent with national nutrition requirements.

(10) Fruit juice beverage A fruit juice beverage was produced with the formulation shown in Table 20.

(11) Capsule A composition (300 mg) shown in Table 21 was encapsulated in a capsule to produce a capsule.

(12) Tablets The composition shown in Table 22 (1 tablet = 250 mg) was tableted to produce tablets.

(13) Food The composition shown in Table 23 (1 tablet = 1000 mg) was tableted to produce a chewable tablet food.

(14) Beverage The composition shown in Table 24 was mixed to produce a beverage.

(14) Beverage The sports drink of the present invention was prepared with the formulation and conditions shown in Table 25. The storage stability and flavor of this beverage were good.

Claims (6)

Endurance improver as an active ingredient Resuberatoro Le.   The endurance improver according to claim 1, which suppresses fatigue. Motor function improving agent comprising as an active ingredient Resuberatoro Le. Energy metabolism activator as an active ingredient Resuberatoro Le. Muscular strength agent as an active ingredient Resuberatoro Le. An active ingredient Resuberatoro Le, endurance reduction, muscle atrophy, inhibit physiological changes that occur with aging selected from energy metabolism reduction and muscle weakness, aging inhibitors.