CN115645312B - Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method thereof - Google Patents
Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method thereof Download PDFInfo
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- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 93
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 89
- 241000220690 Sargassum pallidum Species 0.000 title claims abstract description 84
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 239000002537 cosmetic Substances 0.000 title claims abstract description 8
- 230000002087 whitening effect Effects 0.000 title claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 43
- 239000000843 powder Substances 0.000 claims description 34
- 238000000605 extraction Methods 0.000 claims description 33
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- 238000001704 evaporation Methods 0.000 claims description 7
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- 238000011068 loading method Methods 0.000 claims description 6
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- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
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- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims 1
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- 229960004705 kojic acid Drugs 0.000 description 3
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 3
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- JPYHHZQJCSQRJY-UHFFFAOYSA-N Phloroglucinol Natural products CCC=CCC=CCC=CCC=CCCCCC(=O)C1=C(O)C=C(O)C=C1O JPYHHZQJCSQRJY-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 2
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- QCDYQQDYXPDABM-UHFFFAOYSA-N phloroglucinol Chemical compound OC1=CC(O)=CC(O)=C1 QCDYQQDYXPDABM-UHFFFAOYSA-N 0.000 description 2
- 229960001553 phloroglucinol Drugs 0.000 description 2
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
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- 235000017519 Artemisia princeps Nutrition 0.000 description 1
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- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
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- 229920002302 Nylon 6,6 Polymers 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 239000003960 organic solvent Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
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- 239000000419 plant extract Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 238000003815 supercritical carbon dioxide extraction Methods 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Compounds Of Unknown Constitution (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparing a whitening cosmetic and a preparation method thereof.
Description
Technical Field
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparation of whitening cosmetics and a preparation method thereof.
Background
The seaweed generally contains bioactive substances with special functions such as phenols, indoles, terpenes and the like under special ecological environment, and is an important source of marine natural medicines. The seaweed polyphenol is a polyphenol compound extracted from seaweed, the structural unit of the seaweed polyphenol is phloroglucinol, and the oligomeric phloroglucinol is generally considered to be further polymerized to form tannin with higher molecular weight.
The research and application of algae polyphenols are based on extraction and separation technology of polyphenols, so that the effective extraction and separation technology and analysis method of algae polyphenols are an indispensable link in the research of polyphenols. The general production method of seaweed polyphenol is to use seaweed as raw material, and adopts solvent extraction methods, such as methanol extraction, ethanol extraction, water extraction and the like, and has the defects of long time, high temperature, low extraction rate, low extract purity and the like, which is not beneficial to industrial production. In order to further improve the polyphenol extraction rate, some physical auxiliary extraction methods are also widely applied to the seaweed polyphenol extraction process, such as high-pressure auxiliary extraction, microwave auxiliary extraction, ultrasonic auxiliary extraction, enzymatic auxiliary extraction, supercritical carbon dioxide extraction and the like. Given the high sugar concentration and the abundance of other components (e.g., pigments, proteins, fatty acids, etc.) in the caraway, these components may be incorporated into the caraway polyphenols during the preparation process, thereby affecting the quality of the extracted polyphenols. Therefore, the further purification of the ultrasonic-assisted ethanol-extracted sargassum pallidum polyphenol is needed in combination with other technologies.
As a main active substance in seaweed and having various biological activities, algal polyphenols are being gradually studied and explored. The seaweed polyphenol has been reported to have antioxidant, hypoglycemic, anticancer, anticoagulant, antiinflammatory, and enzyme (angiotensin converting enzyme, matrix metalloproteinase, acetylcholinesterase and butyrylcholinesterase, tyrosinase, hyaluronidase) inhibitory activities. In the research, most of the biological activity studies are conducted on non-human animals, including in vitro and in vivo animal models, but due to the difference of enzyme catalysis centers and the complexity of organisms, the applicability and safety of the seaweed polyphenol as a drug are still to be further studied.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a novel marine plant extract which is further purified by adopting a separation material and can be used for preparing whitening cosmetics aiming at the defects of the prior art.
In order to achieve the purpose of the invention, the following technical means are specifically adopted:
application of sargassum pallidum polyphenol in preparing whitening cosmetic is provided.
Application of sargassum pallidum polyphenol in preparing cosmetic with tyrosinase inhibiting effect is provided.
The preparation method of the sargassum pallidum polyphenol comprises the following steps:
(1) Cleaning fresh sargassum pallidum, lyophilizing, pulverizing, and sieving to obtain sargassum pallidum powder;
(2) Accurately adding the sargassum pallidum powder into an ethanol water solution with the volume concentration of 30-80% according to the feed-liquid ratio of 1:15-60 (w/v), fully mixing and soaking;
(3) Extracting the sargassum pallidum polyphenol by ultrasonic at 40-80 ℃ for 20-200 min, filtering after extraction, and collecting filtrate;
(4) Repeating the step (3) for 1-3 times, and combining the filtrates;
(5) Evaporating and concentrating the filtrate at 30-60 ℃ to obtain a crude extract of the sargassum pallidum polyphenol;
(6) Primarily purifying the crude extract by using macroporous resin or polyamide to obtain a crude product of the sargassum pallidum polyphenol;
(7) Further purifying the crude product of the sargassum pallidum polyphenol by using sephadex;
(8) And (5) performing vacuum freeze drying to obtain the sargassum pallidum total polyphenol.
Preferably, the feed liquid ratio in the step (2) is preferably 1:25-45, more preferably 1:31-1:40. The ethanol concentration is preferably 40% to 60%, more preferably 45% to 55%.
Preferably, the extraction temperature in the step (3) is preferably 50 to 70 ℃, and more preferably 60 to 70 ℃. The ultrasonic time is preferably 20 to 100 minutes, more preferably 20 to 53 minutes.
Preferably, the concentration temperature in the step (5) is preferably 40-50 ℃.
Preferably, the macroporous resin type selected for the preliminary purification of the crude extract of the sargassum pallidum polyphenol in the step (6) is preferably LS-305A, AB-8, XDA-7, BS-45, NKA-9, ADS-17, ADS-21, HPD100, DA201 and XAD16N, more preferably LS-305A, DA201 and XAD16N; the polyamide resin is preferably nylon-6 and nylon-66, more preferably nylon-6.
Preferably, the sephadex type of the further purified polyphenol sample of the step (7) is preferably G-100, G-75, G-25, G15, LH-20, more preferably LH-20.
Advantageous effects
1. Under the auxiliary action of ultrasonic wave, extracting polyphenol by adopting an ethanol aqueous solution, carrying out suction filtration and rotary evaporation concentration to obtain a crude extract of the sargassum pallidum polyphenol, purifying, and finally freeze-drying to obtain a high-purity polyphenol product.
2. The method has the advantages of short extraction time, simple operation, fewer steps, less loss and lower production cost.
3. The GB/T8313-2008 'Fu Lin Fen reagent colorimetric method' is adopted to measure the polyphenol content, gallic acid is used as a standard substance, a standard curve is established, the sargassum pallidum polyphenol content in the sample is converted through the standard curve, and the calculation is more accurate;
4. vacuum freeze drying can not only keep the quality stability of the sargassum pallidum polyphenol product in the drying process, but also effectively remove the taste of the residual organic solvent, and has good drying and deodorizing effects;
5. compared with the crude extract of the sargassum pallidum polyphenol purified by macroporous resin, the purity of the sargassum pallidum polyphenol obtained by two-step purification is higher.
6. The sargassum pallidum polyphenol obtained by extraction and purification has strong tyrosinase inhibition activity, high purity, good extraction rate and good polyphenol activity maintenance, and is suitable for large-scale industrial production.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The preparation experiment of the sargassum pallidum polyphenol is as follows:
example 1
(1) Fresh sargassum pallidum was freeze-dried for 40 hours and chopped. Then placing the powder into a pulverizer and sieving the powder through a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 45% ethanol water solution according to a feed liquid ratio of 1:40 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, the extraction temperature is 60 ℃, and the filter residues are extracted again under the same condition and are subjected to suction filtration;
(4) And combining the filtrates obtained by the two extractions, and evaporating and concentrating at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extract.
(5) The gallic acid is used as a standard substance, and the GB/T8313-2008 Fu Lin Fen reagent colorimetric method is adopted to measure the polyphenol content as follows: 5.5mg/g dry seaweed.
(6) The crude extract of the sargassum pallidum polyphenol is initially purified by polyamide resin (nylon-6), the sample is loaded at the flow rate of 1.0BV/h, the mixture is stood for adsorption for 1h, the deionized water with 1 column volume is used for washing impurities, the 50% ethanol with 2.5 column volumes is used for eluting, the eluent is collected, the eluent is concentrated by vacuum rotary evaporation at 40-45 ℃ and freeze-dried to obtain the crude pure powder of the sargassum pallidum polyphenol.
(7) Taking the total polyphenol of the sargassum pallidum purified by polyamide resin, precisely weighing, ultrasonically dissolving with a small amount of water, centrifuging, loading the supernatant on a sephadex LH-20 chromatographic column, eluting with water, 50% ethanol and absolute ethanol respectively, performing rotary evaporation and freeze-drying on each component eluent to obtain refined powder of the sargassum pallidum polyphenol, and measuring that the purity of the total polyphenol of the sargassum pallidum which is 50% ethanol component is the highest and is 54.5%.
Example 2
(1) Fresh sargassum pallidum was freeze-dried for 40 hours and chopped. Then placing the powder into a pulverizer and sieving the powder through a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 45% ethanol water solution according to a feed-liquid ratio of 1:35 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, the extraction temperature is 65 ℃, and the filter residues are extracted again under the same condition and are subjected to suction filtration;
(4) And combining the filtrates obtained by the two extractions, and evaporating and concentrating at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extract.
(5) The gallic acid is used as a standard substance, and the GB/T8313-2008 Fu Lin Fen reagent colorimetric method is adopted to measure the polyphenol content as follows: 6.0mg/g dry seaweed.
(6) The crude extract of the sargassum pallidum polyphenol is initially purified by macroporous resin LS-305A, the sample is loaded at the flow rate of 1.0BV/h, the mixture is stood for adsorption for 1h, the deionized water with 1 column volume is used for washing the impurities, the 50 percent ethanol with 2.5 column volumes is used for eluting, the eluent is collected, the eluent is concentrated by vacuum rotary evaporation at the temperature of 40-45 ℃ and the sargassum pallidum polyphenol primary pure product powder is obtained by freeze-drying.
(7) Taking the total polyphenol of the sargassum pallidum purified by macroporous resin, precisely weighing, ultrasonically dissolving by using a small amount of water, centrifuging, loading the supernatant on a sephadex LH-20 chromatographic column, eluting by using water, 50% ethanol and absolute ethanol respectively, performing rotary evaporation and freeze-drying on each component eluent to obtain refined powder of the sargassum pallidum polyphenol, and measuring that the purity of the total polyphenol of the sargassum pallidum which is the 50% ethanol component is the highest and is 66.5%.
Example 3
(1) Fresh sargassum pallidum was freeze-dried for 40 hours and chopped. Then placing the powder into a pulverizer and sieving the powder through a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to a feed-liquid ratio of 1:35 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 500W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, the extraction temperature is 65 ℃, and the filter residues are extracted again under the same condition and are subjected to suction filtration;
(4) And combining the filtrates obtained by the two extractions, and evaporating and concentrating at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extract.
(5) The gallic acid is used as a standard substance, and the GB/T8313-2008 Fu Lin Fen reagent colorimetric method is adopted to measure the polyphenol content as follows: 6.5mg/g dry seaweed.
(6) The crude extract of the sargassum pallidum polyphenol is primarily purified by macroporous resin DA-201, the sample is loaded at the flow rate of 1.0BV/h, the mixture is stood for adsorption for 1h, the deionized water with 1 column volume is used for washing the impurities, the 50 percent ethanol with 2.5 column volumes is used for eluting, the eluent is collected, the eluent is concentrated by vacuum rotary evaporation at the temperature of 40-45 ℃ and the sargassum pallidum polyphenol primary pure product powder is obtained by freeze-drying.
(7) Taking the total polyphenol of the sargassum pallidum purified by macroporous resin, precisely weighing, ultrasonically dissolving by using a small amount of water, centrifuging, loading the supernatant on a sephadex LH-20 chromatographic column, eluting by using water, 50% ethanol and absolute ethanol respectively, performing rotary evaporation and freeze-drying on each component eluent to obtain refined powder of the sargassum pallidum polyphenol, and measuring that the purity of the total polyphenol of the sargassum pallidum which is the 50% ethanol component is the highest and is 56.5%.
Example 4
(1) Fresh sargassum pallidum was freeze-dried for 40 hours and chopped. Then placing the powder into a pulverizer and sieving the powder through a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to a feed-liquid ratio of 1:35 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 500W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, the extraction temperature is 65 ℃, and the filter residues are extracted again under the same condition and are subjected to suction filtration;
(4) And combining the filtrates obtained by the two extractions, and evaporating and concentrating at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extract.
(5) The gallic acid is used as a standard substance, and the GB/T8313-2008 Fu Lin Fen reagent colorimetric method is adopted to measure the polyphenol content as follows: 7.5mgGAE/g dry seaweed.
(6) The crude extract of the sargassum pallidum polyphenol is initially purified by macroporous resin LS-305A, the sample is loaded at the flow rate of 1.0BV/h, the mixture is stood for adsorption for 1h, the deionized water with 1 column volume is used for washing the impurities, the 50 percent ethanol with 2.5 column volumes is used for eluting, the eluent is collected, the eluent is concentrated by vacuum rotary evaporation at the temperature of 40-45 ℃ and the sargassum pallidum polyphenol primary pure product powder is obtained by freeze-drying.
(7) Taking the total polyphenol of the sargassum pallidum purified by macroporous resin, precisely weighing, ultrasonically dissolving by using a small amount of water, centrifuging, loading the supernatant on a sephadex LH-20 chromatographic column, eluting by using water, 50% ethanol and absolute ethanol respectively, performing rotary evaporation and freeze-drying on each component eluent to obtain refined powder of the sargassum pallidum polyphenol, and measuring that the purity of the total polyphenol of the sargassum pallidum which is the 50% ethanol component is the highest and is 70.5%.
Comparative example 1
(1) Fresh sargassum pallidum was freeze-dried for 40 hours and chopped. Then placing the powder into a pulverizer and sieving the powder through a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to a feed-liquid ratio of 1:35 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, and the extraction temperature is 60 ℃;
(4) Repeating the extraction for 2 times, and combining the supernatants;
(5) Evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃ to obtain the caraway polyphenol extract.
(6) The content of polyphenol measured by GB/T8313-2008 Fu Lin Fen reagent colorimetric method is as follows: 7.57mg/g dry seaweed.
(7) Purifying by LS-305A macroporous resin dynamic adsorption, wherein the operation steps are that the flow rate of 50ml of the sargassum pallidum polyphenol extract is 0.5BV/h, the mixture is stood for 1 hour after the sample is added, 1BV water and 1BV 5% ethanol are used for removing impurities respectively, and 2.5BV 50% ethanol is used for eluting. And (3) vacuum freeze-drying the eluent to obtain the sargassum pallidum polyphenol extract, wherein the purity of the total polyphenol is 20.4%. The purity of the polyphenols was significantly reduced compared to example 4.
Example 5
Experiment of inhibition of tyrosinase by the extract of sargassum pallidum polyphenol obtained in example 4:
the effect of the test sample on tyrosinase activity was determined by dopa staining.
60uL of phosphate buffer solution with pH of 6.8 and 20uL of tyrosinase solution with activity of 50U/mL are taken, 40uL of sargassum pallidum polyphenol with different mass concentrations is added, uniformly mixed and stood for 8min, 40uL of L-dopa solution with concentration of 0.01mol/L is added, and after 2min of reaction, the absorbance value of the system is measured at 475nm wavelength. Phosphoric acid buffer was used as a blank control and kojic acid was used as a positive control. Experiments were performed 3 times in parallel. Measuring the influence of different concentration sample solutions on tyrosinase inhibition activity, calculating inhibition rate, plotting inhibition rate against concentration by using Graphpad prism 8 software, fitting regression equation, and respectively obtaining half inhibition mass concentration (IC) of sargassum pallidum polyphenol and kojic acid 50 )。
Inhibition (%) = [ [ (a) 1 -A 2 )-(A 3 -A 4 )]/(A 1 -A 2 )]×100
Wherein: a is that 1 Absorbance for the polyphenol group with tyrosinase but without caraway; a is that 2 Absorbance for a polyphenol group free of tyrosinase and sargassum pallidum; a is that 3 Absorbance for polyphenol group containing tyrosinase and sargassum pallidum; a is that 4 Is the absorbance of the caraway polyphenol but without tyrosinase group.
Experimental results:
TABLE 2 Effect of Artemisia princeps extract and kojic acid on tyrosinase activity
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (2)
1. The application of the sargassum pallidum polyphenol in preparing the whitening cosmetics is characterized in that the preparation method of the sargassum pallidum polyphenol comprises the following steps:
(1) Freeze-drying fresh sargassum pallidum for 40 hours, shearing the sargassum pallidum, and then putting the sargassum pallidum into a pulverizer and sieving the sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to a feed-liquid ratio of 1:35 (w/v), mixing well and soaking;
(3) Extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 500W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, the extraction temperature is 65 ℃, and the filter residues are extracted again under the same condition and are subjected to suction filtration;
(4) Combining the filtrates obtained by the two extractions, and evaporating and concentrating at 40-50 ℃ on a rotary evaporator to obtain a sargassum pallidum polyphenol extract;
(5) Performing primary purification on the crude extract of the sargassum pallidum polyphenol by using macroporous resin LS-305A, loading the sample at a flow rate of 1.0BV/h, standing for adsorption for 1h, washing with deionized water with 1 column volume to remove impurities, eluting with 50% ethanol with 2.5 column volumes, collecting the eluent, performing vacuum rotary evaporation concentration at 40-45 ℃, and freeze-drying to obtain the primary pure product powder of the sargassum pallidum polyphenol;
(6) Taking the primary pure product powder of the sargassum pallidum polyphenol purified by macroporous resin, precisely weighing, ultrasonically dissolving by using a small amount of water, centrifuging, loading the supernatant on a sephadex LH-20 chromatographic column, eluting by using water, 50% ethanol and absolute ethanol respectively, and performing rotary evaporation and freeze-drying on the eluent of each component to obtain the sargassum pallidum polyphenol with 50% ethanol.
2. The use according to claim 1, wherein the sargassum pallidum polyphenol exerts a whitening effect by inhibiting tyrosinase.
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