CN115645312A - Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method of sargassum pallidum polyphenol - Google Patents
Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method of sargassum pallidum polyphenol Download PDFInfo
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- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 100
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 99
- 241000220690 Sargassum pallidum Species 0.000 title claims abstract description 85
- 239000002537 cosmetic Substances 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 230000002087 whitening effect Effects 0.000 title claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 38
- 238000000605 extraction Methods 0.000 claims description 25
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- 239000000287 crude extract Substances 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 4
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- 238000004519 manufacturing process Methods 0.000 claims description 3
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- 239000000243 solution Substances 0.000 description 20
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- 241001474374 Blennius Species 0.000 description 12
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
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- 238000004090 dissolution Methods 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 4
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- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- JPYHHZQJCSQRJY-UHFFFAOYSA-N Phloroglucinol Natural products CCC=CCC=CCC=CCC=CCCCCC(=O)C1=C(O)C=C(O)C=C1O JPYHHZQJCSQRJY-UHFFFAOYSA-N 0.000 description 2
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- 150000002989 phenols Chemical class 0.000 description 2
- QCDYQQDYXPDABM-UHFFFAOYSA-N phloroglucinol Chemical compound OC1=CC(O)=CC(O)=C1 QCDYQQDYXPDABM-UHFFFAOYSA-N 0.000 description 2
- 229960001553 phloroglucinol Drugs 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
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- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 1
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- 229940127219 anticoagulant drug Drugs 0.000 description 1
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- 230000003197 catalytic effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Compounds Of Unknown Constitution (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparing whitening cosmetics and a preparation method thereof.
Description
Technical Field
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparing whitening cosmetics and a preparation method thereof.
Background
The seaweed generally contains bioactive substances with special functions such as phenols, indoles, terpenoids and the like in living under special ecological environment, and is an important source of marine natural medicines. The seaweed polyphenol is a general name of polyphenol compounds extracted from seaweed, the brown seaweed polyphenol is a class of phenolic compounds extracted from brown seaweed, the structural unit of the brown seaweed polyphenol is phloroglucinol, and generally, oligomeric phloroglucinol can be further polymerized to form tannin with higher molecular weight.
Research and application of seaweed polyphenol need to be based on polyphenol extraction and separation technology, so that an effective extraction and separation process and an analysis method of the seaweed polyphenol are indispensable links in polyphenol research. The general production method of algal polyphenol is to use algae as raw material and adopt solvent extraction methods, such as methanol extraction, ethanol extraction, water extraction and the like, has the defects of long time, high temperature, low extraction rate, low extract purity and the like, and is not beneficial to industrial production. In order to further improve the polyphenol extraction rate, some physical auxiliary extraction methods are also widely applied in the extraction process of the seaweed polyphenol, such as high-pressure auxiliary extraction, microwave auxiliary extraction, ultrasonic auxiliary extraction, enzyme auxiliary extraction, supercritical carbon dioxide extraction and the like. Considering the high sugar concentration of sargassum pallidum and the abundance of other components (such as pigments, proteins, fatty acids, etc.), these components may be mixed into sargassum pallidum polyphenol during the preparation process, thereby affecting the quality of the extracted polyphenol. Therefore, the sargassum pallidum polyphenol extracted by the ultrasonic-assisted ethanol needs to be further purified by combining other technologies.
Algal polyphenols are being studied and explored gradually as main active substances in seaweeds and having various biological activities. Studies report that algal polyphenols have activities such as antioxidation, hypoglycemic, anticancer, anticoagulant, anti-inflammatory, enzyme (angiotensin converting enzyme, matrix metalloproteinase, acetylcholinesterase and butyrylcholinesterase, tyrosinase, hyaluronidase) inhibition, and the like. In the research, most of the biological activity studies are conducted in non-human, including in vitro and in vivo animal models, but due to the difference in the catalytic centers of enzymes and the complexity of organisms, the applicability and safety of the use of algal polyphenols as drugs have yet to be further studied.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a novel marine plant extract aiming at the defects of the prior art, and further purify the marine plant extract by adopting a separation material, and the extract can be used for preparing whitening cosmetics.
In order to realize the purpose of the invention, the following technical means are specifically adopted:
application of HAIDAOZI polyphenol in preparing whitening cosmetic is provided.
Application of sargassum pallidum polyphenol in preparing cosmetics with tyrosinase inhibiting effect is provided.
The preparation method of the sargassum pallidum polyphenol comprises the following steps:
(1) Cleaning fresh herba Artemisiae Annuae, lyophilizing, pulverizing, and sieving to obtain herba Artemisiae Annuae powder;
(2) Accurately adding the sargassum pallidum powder into an ethanol water solution with the volume concentration of 30-80% according to the material-liquid ratio of 1: 15-60 (w/v), and fully and uniformly mixing and soaking;
(3) Ultrasonically extracting the sargassum pallidum polyphenol at the extraction temperature of 40-80 ℃ for 20-200 min, carrying out suction filtration after extraction, and collecting filtrate;
(4) Repeating the step (3) for 1-3 times, and combining the filtrates;
(5) Evaporating and concentrating the filtrate at 30-60 ℃ to obtain crude artemisia apiacea polyphenol extract;
(6) Primarily purifying the crude extract by using macroporous resin or polyamide to obtain crude sargassum pallidum polyphenol product;
(7) Further purifying the crude sargassum pallidum polyphenol product by using sephadex;
(8) Vacuum freeze drying to obtain the sargassum pallidum total polyphenol.
Preferably, the feed-liquid ratio in the step (2) is 1: 25 to 45, and more preferably 1: 31 to 1: 40. The ethanol concentration is preferably 40% to 60%, and more preferably 45% to 55%.
Preferably, the extraction temperature in the step (3) is preferably 50 to 70 ℃, and more preferably 60 to 70 ℃. The ultrasonic treatment time is preferably 20 to 100min, and more preferably 20 to 53min.
Preferably, the concentration temperature in the step (5) is preferably 40 to 50 ℃.
Preferably, the model of the macroporous resin selected for the crude extract of the sargassum pallidum polyphenol obtained by the preliminary purification in the step (6) is preferably LS-305A, AB-8, XDA-7, BS-45, NKA-9, ADS-17, ADS-21, HPD100, DA201 and XAD16N, and further preferably LS-305A, DA201 and XAD16N; the polyamide resin type is preferably nylon-6 and nylon-66, and more preferably nylon-6.
Preferably, the sephadex type of the polyphenol sample further purified by the step (7) is G-100, G-75, G-25, G15, LH-20, and is further preferably LH-20.
Advantageous effects
1. Under the auxiliary action of ultrasonic waves, polyphenol is extracted by adopting an ethanol water solution, crude artemisia scoparia polyphenol extracting solution is obtained through suction filtration and rotary evaporation concentration, and a high-purity polyphenol product is obtained through purification and freeze drying.
2. The method has the advantages of short extraction time, simple operation, less steps, less loss and low production cost.
3. Measuring polyphenol content by GB/T8313-2008 Fulin phenol reagent colorimetric method, establishing a standard curve by using gallic acid as a standard substance, converting sargassum pallidum polyphenol content in a sample by the standard curve, and calculating more accurately;
4. vacuum freeze drying can maintain the quality stability of the sargassum pallidum polyphenol product in the drying process, can effectively remove the taste of the residual organic solvent, and has good drying and odor removing effects;
5. compared with the crude extract of the sargassum pallidum polyphenol purified by macroporous resin, the purity of the sargassum pallidum polyphenol obtained by two-step purification is higher.
6. The sargassum pallidum polyphenol obtained by extraction and purification has strong tyrosinase inhibitory activity, high purity, good extraction rate and good polyphenol activity maintenance, and is suitable for large-scale industrial production.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The sargassum pallidum polyphenol preparation experiment is as follows:
example 1
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 45 vol% ethanol water solution according to the material-liquid ratio of 1: 40 (w/v), and fully and uniformly mixing and soaking;
(3) Extracting polyphenol with a numerical control ultrasonic cleaner with ultrasonic power of 100W and ultrasonic frequency of 40kHZ for 40min at 60 deg.C, vacuum filtering, and extracting the residue under the same conditions;
(4) And combining the filtrates obtained by the two extractions, and carrying out evaporation concentration on the combined filtrates at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extracting solution.
(5) The method for determining the polyphenol content by using gallic acid as a standard substance and adopting GB/T8313-2008 'Fulin phenol reagent colorimetric method' comprises the following steps: 5.5mg/g dry seaweed.
(6) Performing primary purification on the crude extracting solution of the sargassum pallidum polyphenol by using polyamide resin (nylon-6), loading at the flow rate of 1.0BV/h, standing for adsorbing for 1h, washing with deionized water with the volume of 1 column to remove impurities, eluting with 50 percent ethanol with the volume of 2.5 columns, collecting eluent, performing vacuum rotary evaporation and concentration at 40-45 ℃, and performing freeze-drying to obtain the sargassum pallidum polyphenol primary pure product powder.
(7) Precisely weighing the total polyphenol of the sargassum pallidum purified by the polyamide resin, performing ultrasonic dissolution by using a small amount of water, centrifuging, loading the supernatant into a sephadex LH-20 chromatographic column, respectively eluting by using water, 50% ethanol and absolute ethanol, performing rotary evaporation and freeze drying on the eluent of each component to obtain refined powder of the polyphenol of the sargassum pallidum, wherein the purity of the total polyphenol of the sargassum pallidum of 50% ethanol component is measured to be 54.5%.
Example 2
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 45% ethanol water solution according to the material-liquid ratio of 1: 35 (w/v), and fully mixing and soaking;
(3) Extracting polyphenol with a numerical control ultrasonic cleaner with ultrasonic power of 100W and ultrasonic frequency of 40kHZ for 40min at 65 deg.C, vacuum filtering, and extracting the residue under the same conditions;
(4) And combining the filtrates obtained by the two extractions, and carrying out evaporation concentration on the combined filtrates at 40-50 ℃ on a rotary evaporator to obtain the sargassum pallidum polyphenol extracting solution.
(5) The gallic acid is used as a standard substance, and the polyphenol content is measured by GB/T8313-2008 & lt & ltFulin phenol reagent colorimetric method & gt: 6.0mg/g dry seaweed.
(6) Primarily purifying the crude extractive solution of sargassum pallidum polyphenol by using macroporous resin LS-305A, loading at the flow rate of 1.0BV/h, standing for adsorption for 1h, washing with deionized water with 1 column volume to remove impurities, eluting with 50% ethanol with 2.5 column volumes, collecting eluent, carrying out vacuum rotary evaporation and concentration at 40-45 ℃, and freeze-drying to obtain sargassum pallidum polyphenol primary pure product powder.
(7) Precisely weighing the total polyphenol of the sargassum pallidum purified by the macroporous resin, performing ultrasonic dissolution by using a small amount of water, centrifuging, loading the supernatant into a sephadex LH-20 chromatographic column, respectively eluting by using water, 50% ethanol and absolute ethanol, performing rotary evaporation and freeze drying on the eluent of each component to obtain refined powder of the total polyphenol of the sargassum pallidum, wherein the purity of the total polyphenol of the sargassum pallidum of 50% ethanol component is the highest and is 66.5%.
Embodiment example 3
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1: 35 (w/v), and fully mixing and soaking;
(3) Extracting polyphenol with a numerical control ultrasonic cleaner at ultrasonic power of 500W and ultrasonic frequency of 40kHZ for 40min at 65 deg.C, vacuum filtering, and extracting the residue under the same conditions;
(4) And combining the filtrates obtained by the two extractions, and performing evaporation concentration on the combined filtrate on a rotary evaporator at 40-50 ℃ to obtain the artemisia scoparia polyphenol extracting solution.
(5) The gallic acid is used as a standard substance, and the polyphenol content is measured by GB/T8313-2008 & lt & ltFulin phenol reagent colorimetric method & gt: 6.5mg/g dry seaweed.
(6) Carrying out primary purification on the crude extracting solution of the sargassum pallidum polyphenol by using macroporous resin DA-201, carrying out sample loading at the flow rate of 1.0BV/h, standing and adsorbing for 1h, washing out impurities by using deionized water with 1 column volume, eluting by using 50% ethanol with 2.5 column volumes, collecting eluent, carrying out vacuum rotary evaporation and concentration at 40-45 ℃, and freeze-drying to obtain the sargassum pallidum polyphenol primary pure product powder.
(7) Precisely weighing the total polyphenol of the sargassum pallidum purified by the macroporous resin, performing ultrasonic dissolution by using a small amount of water, centrifuging, loading the supernatant into a sephadex LH-20 chromatographic column, respectively eluting by using water, 50% ethanol and absolute ethanol, performing rotary evaporation and freeze drying on the eluent of each component to obtain refined powder of the total polyphenol of the sargassum pallidum, wherein the purity of the total polyphenol of the sargassum pallidum of 50% ethanol component is the highest and is 56.5%.
EXAMPLE 4
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1: 35 (w/v), and fully mixing and soaking;
(3) Extracting polyphenol with a numerical control ultrasonic cleaner with ultrasonic power of 500W and ultrasonic frequency of 40kHZ for 40min at 65 deg.C, vacuum filtering, and extracting the residue under the same conditions;
(4) And combining the filtrates obtained by the two extractions, and performing evaporation concentration on the combined filtrate on a rotary evaporator at 40-50 ℃ to obtain the artemisia scoparia polyphenol extracting solution.
(5) The gallic acid is used as a standard substance, and the polyphenol content is measured by GB/T8313-2008 & lt & ltFulin phenol reagent colorimetric method & gt: 7.5mg GAE/g dry seaweed.
(6) And (2) primarily purifying the crude artemisia apiacea polyphenol extracting solution by using macroporous resin LS-305A, sampling at the flow rate of 1.0BV/h, standing and adsorbing for 1h, washing with deionized water with the volume of 1 column to remove impurities, eluting with 50% ethanol with the volume of 2.5 columns, collecting eluent, carrying out vacuum rotary evaporation and concentration at 40-45 ℃, and freeze-drying to obtain the initial pure artemisia apiacea polyphenol powder.
(7) Precisely weighing the total polyphenol of the sargassum pallidum purified by the macroporous resin, performing ultrasonic dissolution by using a small amount of water, centrifuging, loading the supernatant into a sephadex LH-20 chromatographic column, respectively eluting by using water, 50% ethanol and absolute ethanol, performing rotary evaporation and freeze drying on the eluent of each component to obtain refined powder of the total polyphenol of the sargassum pallidum, wherein the purity of the total polyphenol of the sargassum pallidum of 50% ethanol component is the highest and is 70.5%.
Comparative example 1
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) Accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1: 35 (w/v), and fully and uniformly mixing and soaking;
(3) Extracting polyphenol with a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, and the extraction temperature is 60 ℃;
(4) Extracting repeatedly for 2 times, and mixing the supernatants;
(5) And (4) evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃ to obtain the sargassum pallidum polyphenol extracting solution.
(6) The polyphenol content is measured by GB/T8313-2008 'Fulin phenol reagent colorimetric method': 7.57mg/g dry seaweed.
(7) Purifying by dynamic adsorption with LS-305A macroporous resin at a flow rate of 0.5BV/h for 50ml sample loading of 0.5mg/ml sargassum pallidum polyphenol extract, standing for 1 hr after sample loading, removing impurities with 1BV water and 1BV 5% ethanol respectively, and eluting with 2.5BV 50% ethanol. Vacuum freeze drying the eluate to obtain the Artemisia princeps Pampanini polyphenol extract with total polyphenol purity of 20.4%. The polyphenol purity was significantly reduced compared to example 4.
Example 5
Example 4 inhibition of tyrosinase by sargassum pallidum polyphenol extract:
the effect of the test sample on tyrosinase activity was determined by dopa staining.
Taking 60uL of phosphate buffer solution with pH of 6.8 and 20uL of tyrosinase solution with activity of 50U/mL, adding 40uL of artemisia apiacea polyphenol with different mass concentrations, uniformly mixing, standing for 8min, adding 40ul of 0.01mol/L-dopa solution, reacting for 2min, and measuring the absorbance value of the system at the wavelength of 475 nm. Phosphate buffer solution was used as a blank control, and kojic acid was used as a positive control. The experiments were performed in parallel 3 times. Measuring the influence of sample liquid with different concentrations on tyrosinase inhibitory activity, calculating inhibition rate, adopting Graphpad prism 8 software to plot the inhibition rate on the concentration, fitting regression equation, and respectively calculating half inhibition mass concentration (IC) of sargassum pallidum polyphenol and kojic acid 50 )。
Inhibition (%) = [ [ (a) 1 -A 2 )-(A 3 -A 4 )]/(A 1 -A 2 )]×100
In the formula: a. The 1 Absorbance of tyrosinase in the absence of sargassum polyphenol group; a. The 2 Absorbance in the group without tyrosinase and without sargassum pallidum polyphenol; a. The 3 The absorbance of the group containing tyrosinase and sargassum pallidum polyphenol; a. The 4 The sample is obtained by adding sargassum pallidum polyphenol without tyrosinase absorbance.
The experimental results are as follows:
TABLE 2 Effect of Artemisia annua extract and kojic acid on tyrosinase Activity
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (8)
1. Application of sargassum pallidum polyphenol in preparing whitening cosmetics.
2. Application of sargassum pallidum polyphenol in preparing cosmetics with tyrosinase inhibiting effect.
3. The method for producing sargassum pallidum polyphenol as claimed in any one of claims 1 to 2, wherein the sargassum pallidum polyphenol is produced by the following method:
(1) Cleaning fresh HAIDAOZI, lyophilizing, pulverizing, and sieving to obtain HAIDAOZI powder;
(2) Accurately adding the sargassum pallidum powder into ethanol water solution with the volume concentration of 30-80 percent according to the feed-liquid ratio of 1: 15-60 (w/v), and fully mixing and soaking;
(3) Ultrasonically extracting the sargassum pallidum polyphenol at the extraction temperature of 40-80 ℃ for 20-200 min, carrying out suction filtration after extraction, and collecting filtrate;
(4) Repeating the step (3) for 1-3 times, and combining the filtrates;
(5) Evaporating and concentrating the filtrate at 30-60 ℃ to obtain a crude artemisia scoparia polyphenol extract;
(6) Primarily purifying the crude extract by using macroporous resin or polyamide resin to obtain crude sargassum pallidum polyphenol product;
(7) Further purifying the crude sargassum pallidum polyphenol with sephadex;
(8) Vacuum freeze drying to obtain the sargassum pallidum total polyphenol.
4. The method according to claim 3, wherein the ratio of the feed to the liquid in the step (2) is preferably 1: 25 to 45, more preferably 1: 31 to 1: 40. The ethanol concentration is preferably 40% to 60%, and more preferably 45% to 55%.
5. The method according to claim 3, wherein the extraction temperature in the step (3) is preferably 50 to 70 ℃, and more preferably 60 to 70 ℃. The ultrasonic treatment time is preferably 20 to 100min, and more preferably 20 to 53min.
6. The method according to claim 3, wherein the concentration temperature in the step (5) is preferably 40 to 50 ℃.
7. The preparation method according to claim 3, wherein the crude polyphenol extract of sargassum pallidum primarily purified in step (6) is selected from the resin types of LS-305A, AB-8, XDA-7, BS-45, NKA-9, ADS-17, ADS-21, HPD100, DA201, XAD16N, preferably LS-305A, DA201, XAD16N, and the polyamide resin types are preferably nylon-6 and nylon-66, preferably nylon 6.
8. The method according to claim 3, wherein the sephadex type of the polyphenol sample further purified in the step (7) is preferably G-100, G-75, G-25, G15, LH-20, and more preferably LH-20.
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