CN114366760A - Application of sargassum pallidum polyphenol in preparing medicine for treating diabetes and preparation method thereof - Google Patents
Application of sargassum pallidum polyphenol in preparing medicine for treating diabetes and preparation method thereof Download PDFInfo
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- 241000220690 Sargassum pallidum Species 0.000 title claims abstract description 64
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 56
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 56
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 9
- 239000003814 drug Substances 0.000 title claims abstract description 8
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 238000000605 extraction Methods 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims description 8
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 8
- 238000001704 evaporation Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
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- 229920005989 resin Polymers 0.000 claims description 5
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- 240000000011 Artemisia annua Species 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
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- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 230000005764 inhibitory process Effects 0.000 description 7
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 description 6
- 241001474374 Blennius Species 0.000 description 6
- 229960002632 acarbose Drugs 0.000 description 5
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000004737 colorimetric analysis Methods 0.000 description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 238000009777 vacuum freeze-drying Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- IFBHRQDFSNCLOZ-ZIQFBCGOSA-N 4-nitrophenyl alpha-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC1=CC=C([N+]([O-])=O)C=C1 IFBHRQDFSNCLOZ-ZIQFBCGOSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 241000049464 Artemisia apiacea Species 0.000 description 2
- 235000011570 Artemisia caruifolia var apiacea Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- HIMXGTXNXJYFGB-UHFFFAOYSA-N alloxan Chemical compound O=C1NC(=O)C(=O)C(=O)N1 HIMXGTXNXJYFGB-UHFFFAOYSA-N 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
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- 238000002474 experimental method Methods 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
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- 230000001603 reducing effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OTPDWCMLUKMQNO-UHFFFAOYSA-N 1,2,3,4-tetrahydropyrimidine Chemical compound C1NCC=CN1 OTPDWCMLUKMQNO-UHFFFAOYSA-N 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
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- 230000029087 digestion Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
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- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000003345 hyperglycaemic effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
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- 239000003960 organic solvent Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
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- 239000000758 substrate Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/03—Phaeophycota or phaeophyta (brown algae), e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparing a medicament for treating diabetes and a preparation method thereof.
Description
Technical Field
The invention relates to the technical field of marine organisms, in particular to application of sargassum pallidum polyphenol in preparing a medicament for treating diabetes and a preparation method thereof.
Background
Research and application of seaweed polyphenol need to be based on polyphenol extraction and separation technology, so that an effective extraction and separation process and an analysis method of the seaweed polyphenol are indispensable links in polyphenol research. The general production method of algal polyphenol is to use algal as raw material and adopt solvent extraction method, i.e. firstly, the algal powder and extraction solvent are uniformly mixed, and then extracted at high temp., and immediately filtered under reduced pressure, after extraction, the above-mentioned materials are extracted for 2-3 times, and then dried and pulverized so as to obtain the invented product.
Disclosure of Invention
The invention aims to solve the technical problem of the prior art, provides a novel marine plant extract, adopts macroporous resin for further purification, and can be used for treating and preventing diabetes.
In order to realize the purpose of the invention, the following technical means are specifically adopted:
application of sargassum pallidum polyphenol in preparing medicine for treating diabetes is provided.
Application of sargassum pallidum polyphenol in preparing medicine for inhibiting alpha-glucosidase.
The preparation method of the sargassum pallidum polyphenol comprises the following steps:
(1) freeze-drying the sargassum pallidum for 30-50 hours, and crushing to obtain sargassum pallidum powder;
(2) adding 40-90% ethanol aqueous solution into the sargassum pallidum powder according to the material-to-liquid ratio g/ml of 1: 20-50, and uniformly mixing and soaking;
(3) ultrasonically extracting the sargassum pallidum polyphenol at the extraction temperature of 40-60 ℃;
(4) repeating the step (3) for 1-3 times, and combining the supernatants;
(5) and (3) evaporating and concentrating the supernatant at 40-50 ℃ to obtain the crude artemisia annua polyphenol extract.
Preferably, the ultrasonic power of the step (3) is 50-200W, the ultrasonic frequency is 20-80 kHZ, and the ultrasonic time is 40-60 min.
Preferably, the crude extract is further purified by macroporous resin in the step (5), and then is subjected to vacuum freeze drying to obtain the total polyphenol of the sargassum pallidum.
Advantageous effects
1. According to the invention, when the sargassum pallidum polyphenol is extracted, the ethanol is used as the extracting solution, so that the dissolution of the sargassum pallidum polyphenol is facilitated, the ethanol has a protection effect on the sargassum pallidum polyphenol, and the sargassum pallidum polyphenol is not easy to oxidize, so that an antioxidant is not required to be added additionally, the working procedures are reduced, and the cost is saved;
2. vacuum freeze drying can maintain the quality stability of the sargassum pallidum polyphenol product in the drying process, effectively remove the taste of the residual organic solvent and achieve good drying and deodorizing effects;
3. and (3) adopting macroporous resin for further purification, and improving the purity of the sargassum pallidum polyphenol:
4. the extraction method disclosed by the invention overcomes the adverse effect influences of long time, high temperature, low extraction rate and the like of the traditional extraction method of polyphenol, is simpler and more time-saving, and has higher content of extracted sargassum pallidum polyphenol products.
5. The blood sugar reducing effect of sargassum pallidum polyphenol is verified by adopting two methods of in vivo and in vitro.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The sargassum pallidum polyphenol extraction experiment is as follows:
example 1
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1:40(w/v), and fully mixing and soaking;
(3) extracting polyphenol by using a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40-60 min, and the extraction temperature is 40-60 ℃;
(4) and (3) evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃ to obtain the sargassum pallidum polyphenol extracting solution.
(5) The polyphenol content is measured by GB/T8313-2008 'Fulin phenol reagent colorimetric method': 5.50mg/g dry seaweed.
EXAMPLES example 2
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1:40(w/v), and fully mixing and soaking;
(3) extracting polyphenol with a numerical control ultrasonic cleaner at an extraction temperature of 60 ℃ for 40min under the ultrasonic power of 100W and the ultrasonic frequency of 40 kHZ;
(4) repeatedly extracting for 1 time, and mixing the supernatants;
(5) and (3) evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃, and then carrying out vacuum freeze drying to obtain the crude sargassum pallidum polyphenol extract.
(6) The polyphenol content is measured by GB/T8313-2008 'Fulin phenol reagent colorimetric method': 6.24mg/g dry seaweed.
EXAMPLE 3
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1:40(w/v), and fully mixing and soaking;
(3) extracting polyphenol with a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, and the extraction temperature is 60 ℃;
(4) extracting repeatedly for 2 times, and mixing the supernatants;
(5) and (3) evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃ to obtain the sargassum pallidum polyphenol extracting solution.
(6) The polyphenol content is measured by GB/T8313-2008 'Fulin phenol reagent colorimetric method': 6.17mg/g dry seaweed.
Example 4
(1) Fresh sargassum pallidum is freeze-dried for 40 hours and chopped. Then putting the dried sargassum pallidum into a pulverizer and sieving the dried sargassum pallidum with a 60-mesh sieve to obtain sargassum pallidum powder;
(2) accurately weighing a certain amount of sargassum pallidum powder, adding 50% ethanol water solution according to the material-liquid ratio of 1:35(w/v), and fully mixing and soaking;
(3) extracting polyphenol with a numerical control ultrasonic cleaner, wherein the ultrasonic power is 100W, the ultrasonic frequency is 40kHZ, the extraction time is 40min, and the extraction temperature is 60 ℃;
(4) extracting repeatedly for 2 times, and mixing the supernatants;
(5) and (3) evaporating and concentrating the supernatant on a rotary evaporator at 40-50 ℃ to obtain the sargassum pallidum polyphenol extracting solution.
(6) The polyphenol content is measured by GB/T8313-2008 'Fulin phenol reagent colorimetric method': 7.57mg/g dry seaweed.
(7) Purifying by LS-305A macroporous resin dynamic adsorption, wherein the operation steps are that the sample loading flow rate of 50ml of sargassum pallidum polyphenol extracting solution is 0.5BV/h, standing for 1 hour after the sample loading is finished, removing impurities by using 1BV of water and 1BV of 5% ethanol respectively, and eluting by using 2.5BV of 50% ethanol. And (4) carrying out vacuum freeze drying on the eluent to obtain the sargassum pallidum polyphenol extract.
Example 5
Example 4 inhibition of α -glucosidase by the extracted sargassum pallidum polyphenol extract:
alpha-glucosidase is an important carbohydrate hydrolase of human intestinal epithelial brush protocytes, which promotes digestion and absorption of carbohydrates by hydrolyzing oligosaccharides or disaccharides into monosaccharides. The screening model establishes a reaction system by taking p-nitrophenyl-alpha-D-glucopyranoside (PNPG) as a substrate.
Preparing samples (prepared from sargassum pallidum polyphenol extract and positive control thereof respectively) with solution concentration of 0.1, 0.2, 0.4, 0.8, 1.0mg/mL, adding 50 μ L of each concentration sample solution, setting each concentration to be 3 in parallel, respectively adding 50 μ L of each concentration sample solution into 50 μ L of 0.1U/mL alpha-glucosidase and 120 μ L of PBS buffer solution with pH6.8, mixing, reacting at 37 deg.C for 10min, respectively adding 50 μ L of 5.0mmol/L PNPG, reacting at 37 deg.C for 10min, adding 100 μ L of 0.2mol/LNa2CO3The reaction was stopped and the absorbance (A) was measured at 405 nm. Acarbose is used as a positive control, and a sample background control group (without enzyme) and a blank control group (without sample) under the same system are set at the same time. Measuring the influence of sample solutions with different concentrations on the inhibition activity of alpha-glucosidase, calculating the inhibition rate, adopting origin8.0 software to plot the inhibition rate on the concentration and fitting a regression equation to respectively obtain the half inhibition mass concentrations (IC) of sargassum pallidum polyphenol and acarbose50)。
Inhibition ratio (%) ═ aBlank space-(ASample(s)-ABackground)]*100％/ABlank space
In the formula: a. theBlank spaceThe absorbance value of the blank control group is obtained; a. theSample(s)Is absorbance value of sargassum pallidum polyphenol or acarbose group; a. theBackgroundThe absorbance value of the sample background control group is shown.
The experimental results are as follows:
the results of enzyme activity inhibition in the sample group and the control group are shown in Table 1. Sargassum pallidum polyphenol can also significantly inhibit the activity of alpha-glucosidase compared to the control group.
TABLE 1 Effect of Artemisia annua extract and acarbose on alpha-glucosidase Activity
| Sample (I) | Half maximal inhibitory mass concentration (IC)50)/(mg·mL-1) |
| Artemisia annua polyphenol | 0.107 |
| Acarbose | 0.091 |
Example 6
Example 4 effect of sargassum pallidum polyphenol extract on type 2 diabetic mice:
experimental materials: male mouse, mouse feed, sargassum pallidum polyphenol
The experimental method comprises the following steps:
animal grouping: healthy mice were randomly divided into 5 groups, including blank control group, model group, positive control group (acarbose), artemisia apiacea polyphenol high dose group, and artemisia apiacea polyphenol low dose group, each group consisting of 10 mice.
Establishing a diabetes model: after the mice are purchased, the adaptive words are raised for 1 week, after the mice are fasted and water is not forbidden for 12 hours, 10 healthy mice are randomly selected and injected with normal saline in the abdominal cavity to serve as a blank control group; the rest healthy mice are injected with 150mg/kg of alloxan solution (prepared at present). After 72 hours, selecting the mice with the blood sugar value of more than or equal to 11.1mmol/L as the mice successfully modeled.
Administration: the blank control group and the model group are respectively given with physiological saline; the positive control group is given with 25mg/kg of acarbose tablet solution; the sample groups were given different doses of suspensions of the extract of sargassum pallidum, 30, 60mg/kg as the low dose group and the high dose group, respectively. The mice in each group were recorded for 4 weeks to determine fasting plasma glucose.
TABLE 2 Effect of Hairzia extract on fasting plasma glucose in tetrahydropyrimidine model mice (` X. + -. S, n ═ 10)
The fasting blood glucose levels in the blank animals were significantly lower than in the model group (× P <0.001) compared to the model group, indicating successful modeling of the hyperglycemic model. Compared with the model group, the blood sugar of each administration group is obviously reduced after administration (P <0.001), and the blood sugar reducing effect of each extract is obvious.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. Application of sargassum pallidum polyphenol in preparing medicine for treating diabetes is provided.
2. Application of sargassum pallidum polyphenol in preparing medicine for inhibiting alpha-glucosidase.
3. The method for producing sargassum pallidum polyphenol as claimed in any one of claims 1 to 2, wherein the sargassum pallidum polyphenol is produced by the following method:
(1) freeze-drying the sargassum pallidum for 30-50 hours, and crushing to obtain sargassum pallidum powder;
(2) adding 40-90% ethanol aqueous solution into the sargassum pallidum powder according to the material-to-liquid ratio g/ml of 1: 20-50, and uniformly mixing and soaking;
(3) ultrasonically extracting the sargassum pallidum polyphenol at the extraction temperature of 40-60 ℃;
(4) repeating the step (3) for 1-3 times, and combining the supernatants;
(5) and (3) evaporating and concentrating the supernatant at 40-50 ℃ to obtain the crude artemisia annua polyphenol extract.
4. The preparation method according to claim 3, wherein the ultrasonic power of the step (3) is 50-200W, the ultrasonic frequency is 20-80 kHZ, and the ultrasonic time is 40-60 min.
5. The preparation method of claim 3, wherein the step (5) further purifies the crude extract with macroporous resin, and then vacuum freeze-dries to obtain the total polyphenol of sargassum pallidum.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115645312A (en) * | 2022-10-11 | 2023-01-31 | 江苏海洋大学 | Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method of sargassum pallidum polyphenol |
| CN115998779A (en) * | 2023-01-16 | 2023-04-25 | 青岛科技大学 | Artemisia princeps polyphenol extract and preparation method and application thereof |
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| CN115998779B (en) * | 2023-01-16 | 2024-04-12 | 青岛科技大学 | Artemisia princeps polyphenol extract and preparation method and application thereof |
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