KR20150062625A - Cosmetic Composition Comprising Ishige foliacea Extract, Gracilaria vermiculophylla (Ohmi)Papenfuss Extract, Dictyota coriacea, Pachydictyon coriaceum Extract and Sargassum horneri (Turner) C. Agardh Extract - Google Patents

Abstract

The present invention relates to an extract of a mixture of Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea (Pachydictyon coriaceum ) and Sargassum horneri (Turner) C. Agardh As an active ingredient. The cosmetic composition of the present invention comprising as an active ingredient a mixed extract of a broad-leaved, spiny-tailed, safflower-netted horseshoe, and a horny sea bass is effective for improving skin wrinkles, skin moisturizing effect, skin elasticity improvement, skin texture and antioxidation A cosmetic composition which can be exhibited even more.

Description

(Cosmetic Composition Comprising Ishige foliacea Extract, Gracilaria vermiculophylla (Ohmi) Papenfuss Extract, Dictyota coriacea, Pachydictyon coriaceum Extract and Sargassum horneri (Turner) C. Agardh Extract}

The present invention relates to a method for producing a mixture of Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea (Pachydictyon coriaceum ) and Sargassum horneri (Turner C. Agardh ) The present invention relates to a cosmetic composition containing as an active ingredient, and more particularly to a cosmetic composition comprising a mixture of a broad-leaved, twisted, Improving elasticity, improving skin texture, and antioxidation.

Everyone is healthy, and the desire and desire to look young and beautiful do not spare time and effort. Therefore, many people exercise, prevent and treat health through exercise and medicines, and many cosmetic products are produced and sold for the pursuit of beauty. As the age develops, a healthy and relaxed life is demanded, and as consumers' desire to be beautiful continues to grow, interest in functional cosmetics is increasing. In the past, chemical cosmetics were popular, but in recent years, interest in skin has been amplified due to the era of well-being. Functional cosmetics using diverse natural materials that are hypoallergenic and expected to be effective as cosmetics, ) Are being manufactured. In Article 2 (1) and (2) of the Cosmetics Act, cosmetics refers to products used in the human body to clean and glamorize the human body to add charm, to change appearance brightly, to maintain or promote the health of skin and hair, It is defined as a product that helps skin whitening, wrinkle improvement, protection and so on.

On the other hand, seaweeds (seaweeds) are a large-scale fishery resource which accounts for about 25% of the total aquatic production, and have been widely used as raw materials for edible, medicinal, feed and food industries. Recently, As well as the use of the Internet. The main characteristic of seaweeds is that they are rich in vitamins and minerals. Among them, vitamins are abundant in B1, B2, C and Niacin. Minerals are contained in brown algae, Tongs are good sources of minerals. In addition to seaweeds, algae contain various vitamins and minerals such as taurine, n-3 fatty acid, alginic acid, fucoidan, laminarin, pectin and dietary fiber. Functional ingredients are included. Therefore, various extraction methods have been used for health improvement foods and medicines, and they have been shown to be effective for whitening, anti-aging, and various cosmetic raw materials. The useful components of seaweeds and their efficacy are shown in Table 1.

ingredient efficacy Remarks mineral iodine Promoting metabolism, restoring physical strength, detoxifying effect Formulation action, promoting improvement of bio-defense ability, prevention of hair loss, prevention of skin trouble Brown algae such as kelp calcium Promotion of cerebral nervous system activity, prevention of osteoporosis Brown algae and red algae potassium Hypertensive inhibitory effect Brown algae iron content Prevention of anemia, maintenance of immunity, prevention of hair drying Brown algae and red algae magnesium Vasodilation, congestion and nervousness, prevention of heart disease and kidney stones Various algae Selenium Anticancer effect, active oxygen elimination and antioxidant effect, vascular disease healing, immune function enhancement, skin trouble prevention Various algae Polysaccharide Alginic acid Lowering of cholesterol, lowering of hypertension, activation of intestinal enzymes, removal of harmful substances, dressing action, anti-cancer action, diet effect, inhibition of aging Brown algae such as kelp, seaweed Fucoidan Cancer cell suppression effect, anti-cholesterol effect, arteriosclerosis and heart disease prevention, stroke prevention, immunity enhancement effect Seaweed, seaweed, mackerel Laminarin Prevention of arteriosclerosis, prevention of hyperlipidemia, treatment of heart disease Brown algae vitamin A Maintain eye health, anti-cancer effect, sulfation, growth promotion, healthy skin and hair maintenance, allergy disease improvement Kim, Paralia, Auditory C, E Prevention of skin trouble, antioxidant effect Kim, Tott

As described above, seaweeds can be expected to have various effects due to useful components such as minerals, polysaccharides and vitamins. Especially, when applied to the skin, it has excellent effect on skin such as prevention of skin trouble, antioxidative effect and healthy skin maintenance. Since seaweeds with excellent efficacy in various ways are three oceans, considering the geographical environment of Korea, which is rich in marine resources, there is an increasing need to study cosmetic materials from marine resources including seaweeds.

Conventional patents relating to cosmetic compositions using seaweed extracts are disclosed in Korean Patent Laid-Open Publication No. 10-2013-0016660 A, "Anti-allergy, anti-soothing and moisturizing cosmetic composition containing algae extract composed of mackerel, mugwort, Patent Publication No. 10-1191095 B1 "Method for preparing a cosmetic composition containing an extract of anchovy marinade and cosmetic composition prepared therefrom", Korean Patent Laid-Open Publication No. 10-2012-0000251 A "Cosmetic composition containing sea tangle extract" Publication No. 10-0745023 B1 " Cosmetic composition for acne skin using purplish wool extracts ", and the like. However, there is no cosmetic composition that contains as a main ingredient a mixed extract of Spodoptera sp.

Korean Patent Publication No. 10-2013-0016660 A Korean Patent Registration No. 10-1191095 B1 Korean Published Patent Application No. 10-2012-0000251 A Korean Registered Patent No. 10-0745023 B1

The present inventors have found that a cosmetic composition having excellent effects in skin application such as skin wrinkle improvement, skin moisturizing, skin elasticity improvement, skin texture improvement, skin brightness improvement, skin whitening, skin antioxidation, etc. using the extract of marine resources among various natural extracts As a result of intensive research efforts to develop a composition, it has been found that among the various marine resources, Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea, Pachydictyon coriaceum, Sargassum horneri (Turner) C. Agardh ) as an active ingredient and showed excellent antioxidative effects even at low concentrations when applied to a cosmetic composition. Accordingly, it has been found that when the extract is applied to a cosmetic composition, it exhibits excellent effects on skin by demonstrating skin wrinkle improvement, skin moisturizing effect, skin elasticity improvement, skin texture improvement, skin brightness effect and antioxidative effect It was completed.

Accordingly, an object of the present invention is to provide an extract which exhibits skin wrinkle, skin moisturizing effect, skin elasticity improvement, skin texture improvement, skin brightness effect and antioxidative effect.

Another object of the present invention is to provide a cosmetic composition for skin wrinkle improvement, skin moisturization, skin elasticity improvement, skin texture improvement and skin antioxidation comprising the above-mentioned extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

The present invention provides a cosmetic composition comprising, as an active ingredient, a mixed extract of Spodoptera, Spodoptera sp.

Wherein the cosmetic composition is a mixture of neolpae (Ishige foliacea), kkomul kkosiraegi (Gracilaria vermiculophylla (Ohmi) Papenfuss) , true leather net on the end (Dictyota coriacea, Pachydictyon coriaceum) and Hoe life is Sargassum (Sargassum horneri (Turner) C. Agardh ) It contains an extract as an active ingredient.

The extract is an extract obtained by extracting with water, a solvent selected from methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin and a mixed solvent thereof.

The extract is extracted using at least one extraction method selected from the group consisting of hot extraction at 50 to 120 ° C for 1 to 10 hours, or extraction at 5 to 40 ° C for 1 to 5 days by immersion in an organic solvent.

The extract contains 0.001 to 30.0% by weight, more preferably 0.01 to 10.0% by weight, based on the total weight of the cosmetic composition.

The extract is for skin wrinkle improvement and skin moisturizing.

The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, Or more.

When the extract of the present invention is added to the cosmetic base as an active ingredient, the extract of the present invention exhibits excellent antioxidative effects even at low concentrations, and the skin wrinkle, skin moisturizing effect, skin Improvement of elasticity, improvement of skin texture and antioxidative effect, and thus the effect as a skin cosmetic composition is excellent.

FIG. 1 is a graph showing the DPPH scavenging activity of mixed extracts of Sprockets, Cocktail spp.
FIG. 2 is a graph showing the SOD-like activity measurement results of the mixed extracts of Examples 1-1 to 1-4, such as Spruce, Tortoiseshell, Chamomile root, and Hornfish.

The present invention provides a cosmetic composition comprising, as an active ingredient, a combined extract of Spodoptera sp., Cocktail spp., Chamaecyparis obtusa, and Rhododendron spp.

In the present invention, the term 'extract' means at least one extraction method selected from the group consisting of hot water extraction using a solvent, extraction with a solvent, and extraction using an organic solvent, Extracts obtained by filtration, or extracts obtained by filtration, followed by concentration with a rotary vacuum evaporator to maximally evaporate the solvent, followed by lyophilization.

Ishige foliacea is edible and distributed in all parts of the southern coast, Jeju Island, China and Japan, and its morphological characteristics are leafy, blackish brown. The root is a small half-length, with a short circumferential stem, and the upper part is a broad ideogram or object, and branches off. Sometimes they have air in the tissue at the end of the branch, and they can expand like bubbles. The bubble-like area is yellowish brown, and when dry, it becomes black. It is inhabited mainly on the rocks in the middle of the intertidal zone where the waves are quiet.

Gracilaria vermiculophylla (Ohmi) Papenfus s is distributed all over the world including Korea, Japan, Sakhalin, and Taiwan. It is a red alga that is mainly used for raw material of agar and edible oil because it is rich in unsaturated fatty acid. It is a reddish-brown cartilaginous, whorled, long, short branch with a spiral shape that collects and forms a bundle. The size is 30 ~ 40㎝. Slightly bead-like cells were gathered in one or two layers to form a cortical layer, and the water texture was very large and rounded. Quadri-sporang is made in the parietal tissue. The spermatic sac is a jar shaped deep into the outer part of the vaginal tissue. It grows on stones near sandy terrain.

Dictyota coriacea (Pachydictyon coriaceum ) is a marine alga belonging to the nettle of the algae and is distributed in Korea, Japan, and Taiwan. The body is somewhat fan - shaped and thickly swollen, about 40 cm in length, and split into side branches. The branch is about 15 ㎜ wide and has no sawtooth on the edge. It is dark brown when it is alive, it becomes almost black when it is dried and only yellowish brown at the end. It is usually attached to the rocks from the vicinity of the intertidal zone to the basin.

Sargassum horneri (Turner) C. Agardh is a brown algae belonging to the mating habitat of hats and hats. It lives in the coastal waters of the south coast of Japan and Japan, and is mainly used as feed. The stem from the button-shaped root is elongated to the outer strut, sometimes bifurcated, and branches from the upper part. It gives a lot of small thorns on the stem and petiole of the lower part of the plant. Leaves are thin, spatulate, with midrib and grooved pie from edge to near midrib. The bubble is shaped like a stick with a rounded end and a leaf (foliage) shaped like a leaf at the end. The genital organ is an aphrodite. It grows under the intertidal zone.

The present invention relates to an extract of a mixture of Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea (Pachydictyon coriaceum ) and Sargassum horneri (Turner) C. Agardh As an active ingredient.

According to a preferred embodiment of the present invention, the extract is extracted with a solvent selected from water, methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin and a mixed solvent thereof There is a number.

According to a preferred embodiment of the present invention, the extract is subjected to at least one extraction method selected from the group consisting of hot extraction for 1 to 10 hours at 50 to 120 ° C, extraction at 5 to 40 ° C for 1 to 5 days in an organic solvent Is preferable in terms of the most effective improvement of the yield of the extract.

According to a preferred embodiment of the present invention, the extract extracted from the above method may contain 0.001 to 30.0% by weight, more preferably 0.01 to 10.0% by weight, based on the total weight of the cosmetic composition. When the extract is less than 0.001% by weight based on the total weight of the cosmetic composition, skin wrinkle improvement, skin moisturizing effect, skin elasticity improvement, skin texture improvement and antioxidant effect can not be sufficiently obtained. If the weight percentage is exceeded, there is a problem of safety due to cytotoxicity.

The extract prepared from the above method can provide a cosmetic composition for skin wrinkle improvement and skin moisturization because it has excellent skin wrinkle, skin moisturizing effect, skin elasticity improvement, skin texture improvement and antioxidant effect.

The cosmetic composition may also be selected from the group comprising solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, It may be one or more formulations.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

[Example]

Example 1. Manufacture of a mixed extract of broad-leaved, twisted, spruce,

Example 1-1. Hot water extract

The mixture was mixed at a weight ratio of 1: 1: 1: 1, and 5 times of purified water was added to the total weight of the mixture, followed by heating at 105 ° C for 5 hours. When the extraction process is completed, it is filtered with Whatman paper No. 1, concentrated by a rotary vacuum evaporator to remove water, and then freeze-dried to obtain a wrinkled, twisted, 23.42 g (dry weight) of a mixed extract of raw mushroom was obtained.

Examples 1-2. 80% methanol extract

(80% methanol) was added to the total weight of the mixture in a weight ratio of 1: 1: 1: 1, respectively. The extract was filtered with a filter paper and concentrated by using a rotary vacuum evaporator to evaporate the methanol solvent as much as possible. The extract was freeze-dried, 30.07 g (dry weight) of 80% methanol extract of a mixture of Cocktail, chestnut leather, horse chestnut, and horn fish.

Examples 1-3. 70% ethanol extract

(70% ethanol) was added to the total weight of the mixture in a weight ratio of 1: 1: 1: 1, respectively. The extract was filtered through filter paper and then concentrated using a rotary vacuum evaporator to evaporate the ethanol solvent as much as possible. The extract was freeze-dried, 39.21 g (dry weight) of a 70% ethanol extract of a mixture of Cucurbitaceae, chestnut horsetail, and hornblende were obtained.

Examples 1-4. 30% butylene glycol extract

(30% butylene glycol) was added to the total weight of the mixture at a weight ratio of 1: 1: 1: 1, respectively. The resulting extract was filtered through a filter paper to obtain 4.153 kg of a 30% butylene glycol extract of a mixture of a broad-leaved, twig-tailed, chamois leather, About 3.5%).

EXPERIMENTAL EXAMPLE 1. Antioxidant Effect by DPPH (2,2-Diphenyl-1-picrylhydrazyl) Scavenging Activity

The antioxidative effects of the extracts of Examples 1-1, 1-2, 1-3, and 1-4 were evaluated according to the extracts of the broad-leaved, twisted, spruce, To measure the electron donating ability (ability to supply electrons) to the sample, 100 μl of DPPH (2,2-Diphenyl-1-picrylhydrazyl) of 5 μM to 10 -4 M was added to 100 μl of the solution for each concentration of each extract, After incubation for 30 minutes in a 25 ° C incubator, the absorbance was measured at 540 nm using a microplate counter. The electron donating ability was expressed by the absorbance reduction ratio of the sample solution and the control.

The results of the antioxidative effects are shown in Table 2 and FIG.

Example density DPPH scavenging effect (%) Example 1-1 100 mu g / ml 93% Examples 1-2 100 mu g / ml 82% Example 1-3 100 mu g / ml 84% Examples 1-4 0.1% 97%

As shown in the above Table 2 and FIG. 1, the compositions of Examples 1-1, 1-2, 1-3 and 1-4 were 100 μg / ml or 0.1 μg / ml or 0.1 μg / ml or 0.1 μg / % Showed 80% or more DPPH elimination ability.

Experimental Example 2. Measurement of SOD (Superoxide dismutase) -like activity

SOD-like activity was performed as follows. 20 μl of the sample solution of each of the concentrations of the extracts of Examples 1-1, 1-2, 1-3, and 1-4 in accordance with the concentrations of the spruce, spikelets, chestnut leather base, and hoe saengmabong mixed extract were added to the supernatant dismutase assay kit SOD assay kit-WST) was added and mixed with 20 μl of the enzyme working solution. The mixture was incubated at 37 ° C. for 20 minutes in a incubator for 20 minutes, Absorbance was measured at 450 nm using a plate counter. Superoxide dismutase (100 units) was used as an experimental control, and superoxide dismutase - like activity was expressed by the absorbance reduction rate of the sample solution and the control.

The results for SOD (superoxide dismutase) -like activity measurement are shown in Table 3 and FIG. 2 below.

Example density SOD-like inhibition activity (%) Example 1-1 10 mu g / ml 97% Examples 1-2 10 mu g / ml 90% Example 1-3 10 mu g / ml 94% Examples 1-4 0.01% 87%

As shown in the above Table 3 and FIG. 2, the extracts of Examples 1-1, 1-2, 1-3 and 1-4 were mixed with 10 μg / ml or less of the mixed extracts of spruce, 0.01% and SOD - like activity was more than 80%.

Hereinafter, the present invention will be described in more detail with reference to the following Examples, but not limited to: Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea, Pachydictyon coriaceum and Sargassum horneri (Turner C. Agardh ) Experimental examples of a cosmetic composition containing the mixed extract as an active ingredient will be described in more detail, but the cosmetic composition can be applied as various extracts, and the present invention is not intended to be limited thereto but is specifically explained.

[Prescription Example]: Prescription Example of Cosmetic Composition

Prescription Example 1-1: Softening Longevity

Examples of the formulation of the softening water in the cosmetic containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) One Example (1-1) 0.1 2 Polyoxyethylene hardened castor oil 0.4 3 Glycine 3.5 4 Dipotassium glycyrrhizate 0.1 5 1,3-butylene glycol 3.0 6 Sodium hyaruronate 0.1 7 ethanol 5.0 8 Antioxidant 0.1 9 Triethanolamine 0.1 10 EDTA 0.1 11 antiseptic Suitable amount 12 Purified water Balance

Sodium hyaruronate was dispersed in purified water with a propeller mixer (3000 rpm) to prepare a 1% solution. Raw materials 1 to 8 except for sodium hyaruronate were homogenized at 500 rpm in a water dissolving tank using a propeller mixer, completely dissolved by heating at 75 DEG C, and then cooled to room temperature. Then, the raw materials 9 to 11 were completely dissolved in a separate dissolution tank, and the mixture was put into the above-mentioned water dissolution tank and stirred and mixed. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a softening liquid containing Example 1-1.

Prescription Example 1-2: Nourishing Lotion

Examples of the prescription of the nutritional lotion among the cosmetics containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) One Example (1-1) 0.1 2 glycerin 7.0 3 Sorbitan stearate sucrose cocoate 2.0 4 Mineral oil 4.0 5 Trioctanoin 1.0 6 Stearic Acid 1.0 7 Glyceryl stearate 0.5 8 Sorbitan monostearate 1.0 9 Dimethicone 0.5 10 Antioxidant 0.3 11 Triethanolamine 0.1 12 Carbomer 0.2 13 EDTA 0.1 14 antiseptic Suitable amount 15 Purified water Balance

The carbomer was dispersed at 4000 rpm using a propeller mixer to prepare a 2% solution. Raw materials 1 to 6 were added to an aqueous dissolution tank and dispersed by stirring with a homomixer (2000 rpm) and then heated to 75 캜. The raw material 7-14 was added to the oily melting tank and dissolved by heating to 75 ° C. Then, the oil phase dissolved in the water dissolution tank was charged and emulsified (3000 rpm / 5 minutes) and then cooled to room temperature. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a nutrition lotion containing Example 1-1.

Prescription Example 1-3: Essence

Examples of prescription of essence in cosmetics containing the extract of Example 1-1 are as follows.

ingredient Content (% by weight) One Example (1-1) 0.1 2 glycerin 5.0 3 1,3-butylene glycol 2.0 4 Polyethylene glycol 2.0 5 Carbomer 1.0 6 Sodium hyaruronate 0.1 7 Glycine 3.0 8 Polyacrylamide 2.0 9 Hydroxyethyl cellulose 0.2 10 ethanol 3.0 11 Antioxidant 0.3 12 Triethanolamine 0.1 13 Polyoxyethylene hardened castor oil 1.0 14 EDTA 0.1 15 antiseptic Suitable amount 16 Purified water Balance

Sodium hyaruronate and hydroxyethyl cellulose were dispersed in purified water in a propeller mixer (2000 rpm) to prepare a 1% solution. The carbomer was dispersed in purified water in a propeller mixer (4000 rpm) to prepare a 2% solution. On the other hand, raw materials 1 to 12 were added to an aqueous dissolution tank, stirred and dispersed with a homomixer (2000 rpm), and then heated to 75 캜. The warmed water phase was further cooled to room temperature. The ingredients 13 to 15 were completely dissolved in a separate dissolution tank, and the mixture was added to the above-mentioned water dissolution tank and mixed by stirring. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare an essence containing Example 1-1.

Prescription examples 1-4: Nourishing cream

An example of the formulation of the nutritional cream among the cosmetics containing the extract of Example 1-1 is as follows.

ingredient Content (% by weight) One Example (1-1) 0.1 2 1,3-butylene glycol 3.0 3 glycerin 3.0 4 Hydrogenated lecithin 1.0 5 Octyldodecanol 3.0 6 Trioctanoin 2.0 7 Stearic Acid 1.5 8 Cetostearyl alcohol 2.0 9 Polysorbate 60 1.5 10 Sorbitan sesquioleate 2.0 11 Dimethicone 3.0 12 Antioxidant 0.3 13 Xanthan gum 0.2 14 Triethanolamine 0.1 15 EDTA 0.1 16 antiseptic Suitable amount 17 Purified water Balance

Raw materials 1 to 8 were put into an aqua yard, stirred with a homomixer (2000 rpm), dispersed and then heated to 75 캜. In addition, raw materials 9 to 16 were added to an oily dissolving tank and dissolved by heating to 80 ° C. The oil phase dissolved in the water-dissolving tank was charged and emulsified (3000 rpm / 10 minutes) and then cooled to room temperature. Example 1-1 was added thereto and thoroughly stirred and mixed to prepare a nutritive cream containing Example 1-1.

Prescription Example 1-5: Pack

An example of the formulation of the pack in cosmetics containing the extract of Example 1-1 is as follows.

ingredient Content (% by weight) One Example (1-1) 0.1 2 glycerin 7.0 3 1,3-butylene glycol 3.0 4 Squalene 3.0 5 Dimethicone 3.0 6 Sodium hyaruronate 0.1 7 Glycine 2.0 8 Polyacrylamide 5.0 9 Antioxidant 0.3 10 Triethanolamine 0.1 11 EDTA 0.1 12 antiseptic Suitable amount 13 Purified water Balance

Ingredients 1 to 8 were completely dissolved in a separate dissolving tank and stirred and mixed. Then, Example 1-1 was added thereto, and the mixture was thoroughly stirred and mixed to prepare a pack containing Example 1-1.

[Test Example] Examples of Use of Cosmetic Composition

Test Example 1. Measurement of moisturizing effect

Twenty women aged 30 to 60 years were allowed to use Prescription Examples 1-4 for 4 weeks. Changes in skin moisture were measured using a moisture meter (Corneometer, Courage and Khazaka, Germany) Respectively. The average value of 20 persons was calculated. The higher the moisture change value, the higher the moisturizing effect. The results are shown in Table 9 below.

Test Example 2. Measurement of Elasticity Effect

Twenty women between 30 and 60 years of age were exposed to the use of Prescription Example 1-4 for 4 weeks. Changes in skin elasticity were measured using a flexometer (Cutometer, Courage and Khazaka, Germany) . The average value of 20 persons was calculated. The higher the value of elasticity change (R2), the higher the elasticity effect. The results are shown in Table 9 below.

Test Example 3. Measurement of skin texture improvement effect

Twenty female subjects aged 30 to 60 years were instructed to use Prescription Examples 1-4 for 4 weeks, and skin texture changes were measured using a skin texture analyzer (Visioscan, Courage and Khazaka, Germany). The results are shown in Table 9 below.

Test Example 4. Measurement of wrinkle improvement effect

Twenty women aged 30 to 60 years were allowed to use the prescription examples 1-4 on both wrinkles of the eye for 4 weeks. The replica of the eye wrinkles was imaged using an image analyzer (SV600, Courage and Khazaka , Germany) was used to measure the depth of wrinkles. The results are shown in Table 9 below.

Moisturizing elasticity Skin texture wrinkle Before application 23.14 0.4667 22.84 9.42 Two weeks after application 30.04 0.4851 24.22 9.33 After 4 weeks of application 34.41 0.5324 27.99 8.98 Improvement rate (after 2 weeks) 29.82 3.94 6.04 0.96 Improvement rate (after 4 weeks) 48.70 14.08 22.55 4.67

As shown in Table 9, it was found that after four weeks of use of Formulation Examples 1-4, moisturizing, elasticity, skin texture and wrinkle were improved.

Claims (7)

An extract obtained by mixing Ishige foliacea, Gracilaria vermiculophylla (Ohmi) Papenfuss, Dictyota coriacea (Pachydictyon coriaceum ) and Sargassum horneri (Turner) C. Agardh ) ≪ / RTI >
The method according to claim 1,
Wherein the extract is an extract obtained by extracting with water, a solvent selected from methanol, ethanol, hexane, methylene chloride, ethyl acetate, butanol, propylene glycol, butylene glycol, dipropylene glycol, glycerin and a mixed solvent thereof Composition.
The method according to claim 1,
Wherein the extract is extracted using at least one extraction method selected from the group consisting of hot water extraction at 50 to 120 ° C for 1 to 10 hours or extraction at 5 to 40 ° C for 1 to 5 days by immersion in an organic solvent / RTI >
The method according to claim 1,
Wherein the extract comprises 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the extract comprises 0.01 to 10.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the extract is for skin wrinkle improvement and skin moisturizing.
The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, By weight of the cosmetic composition.

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