KR102363238B1 - Cosmetic composition having skin moisturizing, skin calming, anti-oxidant and anti-inflammatory effects which comprise mixed seaweeds - Google Patents

Abstract

The present invention relates to a cosmetic composition having skin moisturizing, soothing, antioxidant and anti-inflammatory effects containing a seaweed mixture as an active ingredient, and more particularly, to a cosmetic composition comprising a seaweed mixture as an active ingredient, wherein the seaweed mixture contains kelp, Chlorella, seaweed, green seaweed, Ecklonia cava, H. squash, chrysanthemum, chlorella, flat green seaweed, banyan seaweed, mondang leaf cap and euglena are respectively extracted, chlorella is fermented, black seaweed extract hydrolyzate, and flat green seaweed hydrolyzate 0.1 to 10.0, respectively. It is characterized by mixing seaweed prepared by mixing in a content range of %.
The cosmetic composition according to the present invention provides a continuous moisturizing effect, and exhibits skin soothing, antioxidant and anti-inflammatory effects, as well as having no skin irritation and side effects.

Description

Cosmetic composition having skin moisturizing, skin calming, anti-oxidant and anti-inflammatory effects which comprise mixed seaweeds

The present invention extracts each of kelp, kelp, green seaweed, seaweed, seaweed, green tea, Ecklonia serrata, turmeric, mother-of-pearl, chlorella, flat-leaved green seaweed, banyan seaweed, mongdang leaf-cap and euglena, fermented chlorella, and extracts black seaweed to make water It relates to a cosmetic composition having skin moisturizing, soothing, antioxidant and anti-inflammatory effects, containing as an active ingredient a seaweed mixture prepared by mixing an equal amount of an extraction, fermentation, and hydrolyzed hydrolyzate obtained by decomposing and hydrolyzing scallops will be.

In general, the skin is a part of the body that is directly exposed to the external environment, and when exposed to excessive ultraviolet rays or pollutants, skin irritation such as erythema, edema, itching, and inflammatory reactions are induced. These skin troubles are not only aesthetically problematic, but substances generated during the inflammatory reaction cause pigmentation incidentally, and collagen, elastin, hyaluronic acid, and muco are involved in skin elasticity. It causes skin damage by accelerating the breakdown of mucopolysaccharides.

According to the free radical theory presented by D. Harman in the mid-1950s, free radicals destroy cells, cut the connective tissue of the dermal layer of the skin, or cause cross-linking, making it difficult to maintain moisture in the dermal layer and It causes various problems such as wrinkle formation, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, and acne.

The causes of free radicals are known as phagocytosis of white blood cells, the electron transport system during ATP production in mitochondria, the action of Myeloper Oxide (MPO), ultraviolet rays, tobacco, normal metabolic processes, stress, pollutants, and bacteria. .

The phagocytosis refers to an action in which a cell body takes in a foreign substance or bacteria into its own body and decomposes and detoxifies it.

Of course, the human body has superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol, which are antioxidants (radical scavengers), but the antioxidant system is weakened by age, pollution, UV rays, and stress. It is neutralized, and free radicals gradually increase.

The increased free radicals destroy the connective tissue of the dermis such as collagen, elastin, hyaluronic acid, mucopolysacchride, etc. , skin inflammation, skin wrinkles, acne, and skin cancer.

The skin is structurally divided into three layers: the epidermis, the dermis, and the subcutaneous fat tissue. The epidermis layer is structured from the outside in the order of the stratum corneum, granular layer, stratum corneum, and basal layer, and the stratum corneum is the skin that protects the inside of the skin. It exists on the outermost layer and protects the skin like a brick.

It has been reported that intercellular lipids between keratinocytes act like mortar and constitute a skin barrier (J. Invest. Dermatol. 80 (Suppl.), 44-49. 1983).

Through this role, in normal skin, the stratum corneum of the epidermis contains about 10 to 20% of moisture, allowing the cells and unique proteins that make up the skin to function normally.

In particular, since the surface of the stratum corneum is covered with a thin natural protective film made of sebum from the sebaceous glands and sweat from sweat glands, evaporation of moisture can be prevented.

On the other hand, when the moisture retention function of the epidermal layer is lost, the moisture in the stratum corneum is lowered to less than 10%, the elasticity and flexibility of the skin disappear, and the skin becomes dry and rough. This causes dryness of the skin, which causes inflammation and trouble, and the activity of keratinocytes and fibroblasts decreases. Normal skin moisture cannot be maintained, and both skin elasticity and health are lost.

In addition, the decrease in skin elasticity as the water holding power decreases, the elasticity of collagen and elastin, which supported the pore wall of the skin, decreases, and the power to tighten the pores is weakened and the pores increase. It is very important to maintain the moisture content of the stratum corneum above all else because the skin barrier breaks down due to the increased pores and dryness, and bacteria invade and cause inflammation.

Currently, occlusive agents, which are hydrophobic substances such as petrolatum, lanolin, cocoa butter, mineral oil, jojoba oil, shea butter, and silicone, are used to prevent moisture loss in the skin. About 20 to 50 kinds of general raw materials such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil and sodium hydroxide are used. When the above raw materials are added to the product, skin irritation as well as immune reactions such as itchiness, allergy, and skin inflammation are accompanied by an increase in the stratum corneum as well as transepidemal water loss, which causes skin inflammation.

Accordingly, the conventional moisturizing agent contained in the cosmetic composition has a limitation in obtaining a skin moisturizing effect, a protective effect, etc. as it is accompanied by a side effect of causing trouble accompanied by irritation and inflammation to the skin.

To compensate for this, a component with a structure similar to sebum, a natural moisturizing factor (NMF), a polyol of a trihydric alcohol having three hydroxyl groups (-OH), etc. were added to act as a moisturizer.

However, due to the inherent viscosity and physical properties of polyols, there is a disadvantage that it feels sticky or snowy. In addition, depending on geographical or environmental conditions, it may cause stickiness or discomfort in the feeling of use due to the effect of temperature and humidity, and in some cases Conversely, there was also a problem of dehydration.

In order to solve this problem, we have recently developed bioactive materials derived from nature, from animals and plants to microorganisms, and introduced them into cosmetics to maintain and improve the skin's own natural defense function, thereby suppressing moisture loss in the skin and enhancing elasticity and moisturizing power. The R&D of cosmetics is being actively promoted.

Therefore, in this study, it is essential to develop a bioactive material that moisturizes the skin, soothes the skin, inhibits the induction of antioxidants and inflammation from seaweed, and a stable cosmetic for moisture retention.

Korean Patent Laid-Open Publication No. 10-2013-0023326 (published on March 7, 2013), "Cosmetic composition with excellent anti-allergic, soothing and moisturizing effect containing seaweed extract composed of caper, agar agar, and shiitake"

Accordingly, the present invention extracts kelp, kelp, green seaweed, seaweed, green tea, Ecklonia, shiitake, mother-of-pearl, chlorella, flat-leaved green seaweed, banyan leaf laver, mondang leaf hat-and-leaf, euglena, fermented chlorella, and extracted black seaweed. It is to provide skin moisturizing, soothing, antioxidant and anti-inflammatory effects containing a seaweed mixture prepared by mixing the same amount of extraction, fermentation, and hydrolyzed hydrolyzate obtained by hydrolyzing and hydrolyzing flat green seaweed as an active ingredient. .

In order to achieve the above object, the present invention provides a kelp extract, green seaweed extract, serrata extract, seaweed extract, green tea extract, Ecklonia radix extract, Japanese radish extract, mother-and-pearl extract, chlorella extract, flat-leaved green leaf extract, bald eagle extract, mondang leaf A cosmetic with moisturizing, soothing, antioxidant and anti-inflammatory effects containing a mixture of seaweeds, characterized in that it contains a mother and child extract, euglena extract, chlorella fermented product, hydrolyzed sea buckthorn extract, and hydrolyzed serrata as an active ingredient A composition is provided.

In addition, the above kelp extract: kelp extract: seaweed extract: seaweed extract: green tea extract: persimmon persimmon extract: shiitake extract: capsicum extract: chlorella extract: flat-leaved green leaf extract: serrata extract: mondang leaf cap extract: chlorella extract: Fermented product: Hydrolyzed sea buckthorn extract: The mixing weight ratio of hydrolyzed flat green seaweed is 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 5: 0.1 to 5.

In addition, the extract is characterized in that it is extracted using any one or a mixture of two or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and ethylene glycol.

In addition, the cosmetic composition is a lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact It is characterized in that it is formulated in the form of

The seaweed mixture according to the present invention provides continuous skin moisturizing and soothing effects, exhibits excellent anti-inflammatory effects, and has an excellent effect on skin improvement without skin irritation and side effects.

1 is a graph showing the skin moisturizing improvement effect of Comparative Examples 1 and 2 and Preparation Examples 1 to 17
Figure 2 is a graph for the evaluation of the amount of transdermal water loss of Comparative Examples 1 and 2 and Preparation Examples 1 to 17
3 is a graph showing the skin soothing effect of Comparative Examples 1 and 2 and Preparation Examples 1 to 17;
4 is a graph showing the free radical scavenging effect of Examples 1 to 17;
5 is a graph showing the anti-pollution effect of Examples 1 to 17

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

The present invention is a kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf cap extract, chlorella extract, It contains fermented chlorella fermented product, hydrolyzed blue-green seaweed extract and hydrolyzed green seaweed extract, and hydrolyzed blue-green seaweed obtained by hydrolysis of green seaweed as active ingredients to moisturize, calm, antioxidant and anti-inflammatory effects on the skin. It provides a cosmetic composition having.

The above "kelp ( Laminaria japonica )" is an aquatic resource belonging to the Laminaria family among brown algae plants. It is known to have excellent nephritis, anti-cancer and anti-aging effects.

In addition, it has been reported to have excellent anti-inflammatory and anticancer effects because it contains polysaccharides such as alginic acid, fucoidan, and laminaran (Kim Yunsu et al., Journal of the Korean Society of Biotechnology and Biotechnology, 2011, 26: 112- 118).

The above "Japanese green onion ( Ulva lactuca )" is an algae belonging to the genus Galvanidae in the order Galvanidae, and it is a kind of green algae that grows by attaching it to rocks or stones. The content of carbohydrate and crude protein, including fiber, is nutritionally excellent.Based on these ingredients, it has been confirmed to have excellent antioxidant, antibacterial, and anti-inflammatory effects (Kim In-hye et al., Korean Society of Environmental Mutagenic Carcinogens, 2006, 26(2) ), p48-52).

The "hearing ( Codium) Fragile )" is a green algae, Ulvophyceae, Bryopsidales, Codiaceae, Codium, fragile, and has a dark green color. The board is used for food in Korea, China, Japan, and Southeast Asia, etc., and acrylic It has been reported to have high antibacterial, anticancer, antimutagenic and immune activity because it contains a large amount of acid (Kim Minseon et al., KSBB. 2018. 33(2): 89-94).

The above "Wakame ( Undaria ) pinnatifida )" is a brown algae belonging to the seaweed family of the order Seaweed, mainly distributed in the entire coast of Korea and Northeast Asia, and is known as a special seaweed in the Far East. In particular, minerals such as Na, K, Ca, Mg, P, S Rich in dietary fiber, linoleic acid, and vitamins, it is known to be effective in antioxidant, anti-inflammatory, cholesterol lowering, blood pressure control and blood circulation improvement (Dahyun Jung et al., Journal of Applied Biochemistry of Korea, 2012, 55(4), p221-225) .

The above " Salicornia Herbacea )" is an annual herb belonging to the family Mycobacterium, and refers to a halophyte that inhabits beaches, coastal sand dunes, salt fields, and waste salt fields. Since it contains a large amount of flavonoids, it has been reported that it not only has an antioxidant effect but also reduces blood cholesterol and blood lipids (Somi Jeon et al., Korean Society of Cosmetics, 2007, 33(3), p145-152).

The above " Ecklonia cava )" is a seaweed of the brown algae plant, seaweed, kelp, and seaweed, which grows over 1m. In Korea, it is mainly distributed around Jeju-do and the southern coast. Ecklonia cava contains components such as carotenoid, fucoidan and phlorotannin, so it has antioxidant, anti-inflammatory, and anti-cancer properties. , anti-allergy, cell protection, whitening and anti-wrinkle effects are known to be excellent (Yoo Cha-Young et al., 2015, Journal of the Korean Society of Cosmetics, 2015, 41(3), p287-294).

The above " Hizikia fusiformis )" is a marine resource plant that mainly inhabits coastal areas in Korea. et al., Journal of Life Sciences, 2012, 22(7), p889-896).

The "hat" ( Sarassum) fulvellum )" is a hard-to-digest perennial brown algae belonging to the genus Mosquito, which is a representative edible seaweed that forms marine forests off the coast of Korea. It has been reported to be effective against cancer (Soyoung Yoon et al., Journal of the Korean Society for Food Science and Technology, 2010, 42(2), p155-159).

The " Chlorella vulgaris ) is a type of green algae that is classified as a single-celled plant and belongs to the class Chlorlhycea, the order Chlorococcm, and the genus Chlorella, and as a species, vulgaris, pyrenoidosa, and ellipsoidea are known. Chlorella contains nutrients such as protein, vitamins, dietary fiber, and minerals. It has been confirmed to be effective in protecting against microorganisms, viruses, various cancers, and inflammation because it contains a large amount, and it has been confirmed that it protects skin cells by repairing DNA damage caused by UV rays (Kim Kim et al., Asian Beauty Cosmetics Journal, 2014, 42) (4), p479-486).

The above "flat green ( Ulva ) compressa Linnaeus )" belongs to the class Ulvophyceae, the order Ulvales, the genus Ulva, and has the scientific name (synonym) of Enteromorpha compressa Nees, and has a pale green or light green color. It has chloroplasts and one to several pyrenoids, and it is reported to have antioxidant and anti-inflammatory effects by producing a polyphenolic substance called phlorotannin (Sunmi Ahn et al., Journal of Life Sciences, 2011) , 21(4), p576-583).

The " Pyropia yezoensis ( Ueda )" is a red algae belonging to the phylum Rhodophy and Bangiophyceae. As an edible resource in Asia, including Korea, China, and Japan, it contains abundant dietary fiber, vitamins, and nutrients such as minerals. In particular, it is reported to have excellent antioxidant activity because it contains polyphenols (Namho Lee, Ogirim et al., Jeju Life Science Research, 2000, 3, p95-101), and betaine is reported to lower blood cholesterol. , Porpiran, a polysaccharide containing about 10% of dried seaweed, has been reported to have excellent antitumor and antioxidant activity (Jiyoung Lee, Jeongwook Choi, Minkyung Lee, Youngmin Kim, Inhye Kim, Taekjeong Nam et al., Journal of the Korean Society for Fisheries Science, 2014, 47 (6) ), p757-764).

The "dumpling bead cap ( Sargassum Muticum )" is a brown algae belonging to the Phaeophyceae, Fuclaes, and Sargassum, and mainly inhabits in the subtidal zone in the lower intertidal zone of the east and south coasts. It is also called Mongdang leaf capban, and has been reported to have high antioxidant activity and antibacterial activity due to its rich content of polyphenols and flavonoids (Kim Ji-young, Lee Jung-ah, Kim Gil-nam, Yoon Won-jong, Lee Wook-jae, Park Soo-young, etc., Department of Food and Nutrition of Korea) Journal, 2007, 36(6), P663-669).

The above " Euglena Gracilis )" belongs to Euglenophyta, Euglenophyceae, and Euglenaceae. It has a spiral shape with a diameter of 15 to 500㎛, has chloroplasts in the body, antioxidants, photosynthesis, and lives as flagella without a cell wall. In particular, it is reported that Euglena extract promotes cell proliferation of keratinocytes and increases filaggrin expression (U-cheol Jeong et al. 5).

The "Fermented Chlorella" is Chlorella vulgaris ) refers to a product obtained by fermenting, and the strain used for the fermentation of Chlorella is not particularly limited.

The "hydrolyzed sea buckthorn extract" refers to extracting and hydrolyzing algae ( Ulva lactuca ).

The above "hydrolyzed flat blue seaweed " refers to flat blue compressa Linnaeus ) refers to a product obtained by hydrolysis.

In the present invention, all of the extraction, fermentation, and hydrolides have excellent active effects, and a mixture of at least two or more of the extraction, fermentation, and hydrolyzate, preferably a kelp extract, a seaweed extract, an audi extract, a seaweed extract, and seaweed extract. Mixture of extract, Ecklonia cava extract, H. radish extract, mother and child half extract, chlorella extract, flat leek extract, serrata serrata extract, mondang leaf cap and hemlock extract, euglena extract, chlorella fermented product, hydrolyzed black sea buckthorn extract and hydrolyzed green leek extract was found to have an excellent synergistic effect.

In particular, kelp, green seaweed, seaweed, seaweed, seaweed, seaweed, seaweed, seaweed, kelp containing natural moisturizing factors and natural seaweed polysaccharides such as amino acids, vitamins, minerals and fucoidan to provide skin moisturizing, soothing, antioxidant and anti-inflammatory effects. Extracted from hibiscus, mother and half, chlorella, flat green seaweed, banyan leaf laver, mondang leaf hat and euglena, respectively, fermented chlorella, hydrolyzed by extracting black green seaweed, extracted from hydrolyzed flat green seaweed, fermented, hydrolyzed hydrolyzate A seaweed mixture prepared by mixing the decomposition products in equal amounts was used as an active ingredient.

According to a preferred embodiment of the present invention, according to the present invention, kelp extract, green seaweed extract, auditory extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child banyan extract, chlorella extract, kelp extract, serrata serrata extract, mondang leaf The ratio of the mixture prepared by mixing equal amounts of mother and child extract, euglena extract, chlorella fermented product, hydrolyzed sea buckthorn extract, and hydrolyzed flat green seaweed, respectively, may be included in the following proportions.

Kelp extract: Sea buckthorn extract: Helicopter extract: Seaweed extract: Tung-tung nodule extract: Ecklonia cava extract: Hibiscus extract: Mosquito extract: Chlorella extract: Flat green seaweed extract: Lobster laver extract: Mongdang leaf extract: Euglena extract: The weight ratio of hydrolyzed blue-green seaweed extract and hydrolyzed blue-green seaweed is 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0, or 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 8.0 : 0.1 ~ 8.0, or 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 10.0 : 0.1 ~ 5.0 : It can be composed of 0.1 ~ 5.0.

In the cosmetic composition having skin moisturizing, soothing, antioxidant and anti-inflammatory effects, containing a mixture of seaweed extract, fermentation, and hydrolyzate according to the present invention as an active ingredient, the mixture of seaweed extraction, fermentation, and hydrolyzate in the final form contains at least 0.01 to Contained at 20.0% by weight, more preferably at 0.1 to 10.0% by weight, more preferably at 0.1 to 5% by weight, even more preferably at 1.0% to 5.0% by weight.

When the content of the seaweed extract mixture is less than 0.01% by weight, skin moisturizing, soothing, antioxidant and salt-containing effects are insignificant, and when it is contained in an amount of 20% by weight or more, the effect does not significantly increase.

The cosmetic composition for skin moisturizing, soothing, antioxidant and anti-inflammatory effects according to the present invention is a combination based on scientific basic data on the skin. Seaweeds prepared by extracting banyan seaweed, Mongdang leaf capban, and euglena respectively, fermenting chlorella, extracting and hydrolysing black seaweed, and mixing the same amount of extraction, fermentation, and hydrolyzed hydrolyzate obtained by hydrolyzing flat seaweed When the mixture is used as an active ingredient, long-term efficacy can be realized in terms of skin moisturizing and soothing, and an anti-inflammatory effect can also be realized.

Ingredients included in the cosmetic composition of the present invention include extracts from kelp, kelp, seaweed, seaweed, green tea, Eckloniasis, turmeric, mother-of-pearl, chlorella, flat-leaved seaweed, banyan leaf laver, Mongdang leaf cap and euglena, respectively, according to the present invention. , In addition to the seaweed mixture prepared by fermenting chlorella, hydrolyzing green scallops and hydrolyzing, and mixing the same amount of extraction, fermentation, and hydrolyzed hydrolyzate obtained by hydrolyzing scallops, it contains ingredients commonly used in cosmetic compositions. and conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component can be used by selecting .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Renglycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic ester, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, stearate-4, dicetylphosphate , macadamia oil, carboxyvinyl polymer, xanthan gum, or fatty acid ester of sorbitan may be selected and used.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol, glycerin, butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Suspending agents such as microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, paraffin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or triglyceride Any one or more of Kant may be selected and used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Any one or more of ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be selected and used.

In addition, the efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to the individual's skin condition and condition, and external environmental conditions.

Hereinafter, to help the understanding of the present invention will be described in detail by presenting examples and experimental examples. However, the following examples and experimental examples are only provided for easier understanding of the present invention, and should not be construed as limiting the technical level and scope of the present invention thereby. The Examples and Experimental Examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of kelp extract

Wash 1 kg of kelp in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried kelp, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watt for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 2> Sea buckthorn extract

Wash 1 kg of green scallion in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of a 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried scallops, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 3> Hearing extract

After washing 1 kg of auditory meat in running water, immerse in 10 kg of purified water for 12 hours and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried auditory, and ultrasonic extraction was performed at 50 to 60 ° C., 30 to 60 KHz, 200 to 700 watt for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 4> Seaweed extract

Wash 1 kg of seaweed in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried seaweed, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 5> Green tea extract

Wash 1 kg of green tea in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried seaweed, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 6> Ecklonia cava extract

After washing 1 kg of Ecklonia cava in running water, immerse in 10 kg of purified water for 12 hours, steam for 30 minutes to remove salt, and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried Ecklonia coli, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 7> Tofu extract

Wash 1 kg of shiitake in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried hibiscus, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watt for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 8> Half mother and half extract

Wash 1 kg of mother and child in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried mother and child, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 9> Chlorella extract

1 kg of 70% ethyl alcohol aqueous solution was added to 40 g of chlorella, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 10> Green leaf extract

After washing 1 kg of flat green seaweed in running water, immerse in 10 kg of purified water for 12 hours and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of a 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried scallops, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 11> Bansang pattern stone laver extract

After washing 1 kg of sagittal laver in running water, immerse in 10 kg of purified water for 12 hours, steam for 30 minutes to remove salt, and then wash twice. Next, 1 kg of a 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried porphyria, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 12> Mongdang leaf capban extract

Wash 1 kg of Mongdang leaf hat in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried Mongdang leaf hat, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 13> Euglena extract

Wash 1 kg of Euglena in running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 1 kg of 70% ethyl alcohol aqueous solution was added to 100 g of dehydrated and dried Euglena, and ultrasonic extraction was performed at 50 to 60° C., 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours. Then, it was filtered, concentrated under reduced pressure at 50° C. or lower, and freeze-dried.

<Example 14> Chlorella fermented product

Chlorella extract obtained by adding 1 kg of purified water to 40 g of chlorella is obtained by ultrasonic extraction under the conditions of 50 to 60 ° C, 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours, followed by filtration. Inoculate yeast (saccharomyces cerevisiae), which was pre-cultured and activated by adding yeast extract powder and peptone to the chlorella extract, and fermented at 30-35℃ for 48-72 hours, 100-200rpm, 0.1-0.5vvm air condition, and then 0.2 m, filtered through filter paper, concentrated under reduced pressure, and freeze-dried.

<Example 15> Hydrolyzed Red Sea buckthorn extract

Purified water is added 10 to 50 times (based on the powder weight) to the freeze-dried powder obtained in the same manner as in Example 2, and reacted at 90° C. for 15 minutes to inactivate the activity of its own endogenous enzyme. After completion of the reaction of the red seaweed powder solution, the temperature is lowered to medium temperature, and then, 0.1 to 1.0% of the sample weight is added to the hydrolase, followed by reaction at 300 to 500 rpm for 2 to 4 hours. After the reaction was completed, the hydrolytic enzyme was deactivated again at 90° C. for 15 minutes, and then concentrated under reduced pressure and freeze-dried.

<Example 16> Hydrolyzed flat green

Wash 1 kg of baron green fronds under running water, immerse in 10 kg of purified water for 12 hours, and steam for 30 minutes to remove salt and wash twice. Next, 10 to 50 times (based on the weight of powder) is added to the dehydrated and dried chloroplast, and reacted at 90° C. for 15 minutes to inactivate the activity of the endophytic enzyme itself. After completion of the above reaction, after lowering the temperature to medium temperature, hydrolase is added at 0.1 to 1.0% of the raw material, followed by reaction at 300 to 500 rpm for 2 to 4 hours. After the reaction was completed, the hydrolytic enzyme was deactivated again at 90° C. for 15 minutes, filtered, concentrated under reduced pressure, and freeze-dried.

< Example 17 > kelp extract, Sesame Seed Extract , hearing extract, seaweed extract, green tea extract , Ecklonia extract , Root Extract , half cap extract, Chlorella Extract , flat green leaf extract , spheroidal laver extract, Mongdang Leaf Cap Banana Extract , euglena extract, Chlorella Fermentation , Hi drolyzed red sea buckthorn extract, Hydrolyzed flat blue (16 kinds of extraction, fermentation, hydrolyzate ) preparation of the mixture

As in the above-described embodiment, respectively, kelp extract, green seaweed extract, algae extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child extract, chlorella extract, flat green seaweed extract, banyan leaf extract, mondang leaf cap extract, euglena A mixture was prepared in the form of extract, chlorella fermented product, hydrolyzed sea buckthorn extract, and hydrolyzed serrata.

Each composition ratio is as shown in Tables 1 to 2.

Ingredient name Sample name Preparation Example 1 Preparation 2 Preparation 3 Preparation 4 Preparation 5 Preparation 6 Preparation 7 Preparation 8 Preparation 9 Preparation 10 A kelp extract 0.6 - - - - - - - - - Sesame Seed Extract - 0.6 - - - - - - - - hearing extract - - 0.6 - - - - - - - seaweed extract - - - 0.6 - - - - - - green tea extract - - - - 0.6 - - - - - Ecklonia extract - - - - - 0.6 - - - - Chickweed extract - - - - - - 0.6 - - - Mosquito extract - - - - - - - 0.6 - - Chlorella Extract - - - - - - - - 0.6 - algae extract - - - - - - - - - 0.6 parrot extract - - - - - - - - - - Mongdang Leaf Cap Banana Extract - - - - - - - - - - euglena extract - - - - - - - - - - Chlorella Fermented Product - - - - - - - - - - Hydrolyzed Blueberry Extract - - - - - - - - - - Hydrolyzed Blue Sea Blue Extract - - - - - - - - - - glycerin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Butylene Glycol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 hexanediol 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 B Methylglucose sesquistearate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl Stearate 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 beeswax 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Caprylic/Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 shea butter 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 dimethicone 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 tocopheryl acetate 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 fragrance, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

Ingredient name Sample name Preparation 11 Preparation 12 Preparation 13 Preparation 14 Preparation 15 Preparation 16 Preparation 17 Comparative Example 1 Comparative Example 2 A kelp extract 0.04 Sesame Seed Extract 0.04 hearing extract 0.04 seaweed extract 0.04 green tea extract 0.04 Ecklonia extract 0.04 Root Extract 0.04 half cap extract 0.04 Chlorella Extract 0.04 flat green leaf extract 0.04 Sangbang pattern stone laver extract 0.6 0.04 Mongdang Leaf Cap Banana Extract 0.6 0.04 Euglena Extract 0.6 0.04 Chlorella Fermentation 0.6 0.04 hydrolyzed
Sesame Seed Extract 0.6 0.02 hydrolyzed
flat green leaf extract 0.6 0.02 glycerin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Butylene Glycol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 hexanediol 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 EDTA-2NA 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 B Methylglucose sesquistearate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Glyceryl Stearate 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 beeswax 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Caprylic/Capric Triglycerides 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 shea butter 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 dimethicone 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Tocopheryl Acetate 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 fragrance, preservative a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount

< test example 1 > Skin moisturizing effect

1) Experimental method

The cosmetics prepared in the Preparation Examples and Comparative Examples of Tables 1 to 2 were applied to 30 test subjects (male and female aged 20-45 years) twice a day for 4 weeks on both sides of the face and in the first half of the preparation 1 to 17 and the creams of Comparative Examples 1 and 2 were respectively applied and the skin moisture improvement state before, after 2 weeks, and after 4 weeks was measured using a skin moisture meter Corneometer CM 820 (Courage + Khazaka, Germany) The unit is expressed in an arbitrary unit (Arbitrary Unit, AU) given by the device, and the higher the measured value, the higher the moisture content on the skin surface.

2) Experiment result

The experimental results on skin moisture improvement are shown in Table 3 below.

Sample name Skin moisturizing effect (Au) before application 2 weeks later after 4 weeks Comparative Example 1 54.2 56.1 58.9 Comparative Example 2 54.5 54.8 55.2 Preparation Example 1 54.3 61.9 70.4 Preparation 2 55.1 61.2 71.3 Preparation 3 54.6 60.4 67.6 Preparation 4 54.7 61.0 70.3 Preparation 5 55.3 62.1 70.1 Preparation 6 54.4 60.3 68.1 Preparation 7 54.6 61.1 68.4 Preparation 8 54.9 60.8 68.2 Preparation 9 55.2 60.4 69.6 Preparation 10 55.1 60.6 69.4 Preparation 11 54.8 60.2 67.2 Preparation 12 54.8 60.1 68.4 Preparation 13 54.9 60.8 67.7 Preparation 14 55.3 59.7 66.4 Preparation 15 55.2 59.4 66.2 Preparation 16 55.9 59.9 67.8 Preparation 17 54.9 64.2 74.9

As shown in Table 3 above, the moisturizing effect was measured for the cosmetic compositions prepared according to Preparation Examples 1 to 17 and Comparative Examples 1 to 2, and as a result, kelp extract, green seaweed extract, hearing extract, seaweed extract, seaweed extract, Manufactured by mixing Ecklonia cava extract, H. radish extract, Chlorella extract, flat green seaweed extract, lavender seaweed extract, Mongdang leaf cap and half extract, euglena extract, chlorella fermented product, hydrolyzed black seaweed extract, hydrolyzed flat seaweed extract In Examples 1 to 17, it was confirmed that the moisturizing power was higher than Comparative Examples 1 and 2. In particular, in the case of the mixture of Preparation Example 17, it was confirmed that the moisturizing effect was more excellently increased than the individual raw materials of Preparation Examples 1 to 16.

< test example 2 > transdermal water loss evaluation

1) Experimental method

For each of the cosmetic compositions of Preparation Examples 1 to 17 and Comparative Examples 1 to 2 in Table 1, 30 subjects (male and female aged 20-45 years) set the upper arm as the measurement site, and set the width and length 2 × The test site was marked with a size of 2 cm ² and measured. After applying the cosmetic composition to the test site, it was rubbed well, and the amount of transdermal moisture loss was measured using TEWAMETER TM210 (C+K eletronic GmhH. Germany). Short-term moisturizing effect was evaluated by measuring the moisture increase rate (%) 30 minutes after application, 4 days after application, and 8 days after application, based on the water retention amount of 0 hours before application. During the measurement, the amount of transdermal water loss was measured three times consecutively while keeping the probe vertical for 60 seconds. Measurements were carried out in the same temperature and humidity room, and the higher the value, the greater the loss of moisture.

2) Experiment result

The results for the amount of transdermal water loss are shown in Table 4 below.

Sample name Water loss (%) 30 minutes after application 1 day after application 4 days after application 8 days after application Comparative Example 1 36.47 35.65 35.37 34.92 Comparative Example 2 40.49 40.02 39.47 38.96 Preparation Example 1 25.41 22.49 20.03 18.55 Preparation 2 25.35 22.14 19.87 17.64 Preparation 3 25.61 22.32 21.69 19.72 Preparation 4 25.44 23.13 20.69 18.72 Preparation 5 25.46 23.15 20.71 18.74 Preparation 6 25.19 23.84 21.4 19.43 Preparation 7 25.37 23.32 20.88 18.91 Preparation 8 25.48 23.73 21.29 19.32 Preparation 9 25.42 23.27 20.83 18.86 Preparation 10 25.21 23.28 20.84 18.87 Preparation 11 25.61 24.23 21.79 19.82 Preparation 12 25.53 23.36 20.92 18.95 Preparation 13 25.83 24.11 21.67 19.7 Preparation 14 25.67 24.63 22.19 20.22 Preparation 15 26.17 24.83 22.39 20.42 Preparation 16 27.75 24.15 21.71 19.76 Preparation 17 24.37 19.47 16.23 15.21

As shown in Table 4, as a result of comparing the amount of transdermal moisture loss in Preparation Examples 1 to 17 and Comparative Examples 1 to 2 for 8 days, it was confirmed that the improvement in the amount of loss was the highest in Preparation Example 17 over time. In particular, even when compared to Preparation Examples 1 to 16, kelp extract, green seaweed extract, serrata extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, serrata serrata extract, mondang It was confirmed that the composition containing a mixture of leaf cap extract, euglena extract, chlorella fermented product, hydrolyzed serrata extract, and hydrolyzed serrata was significantly superior.

<Test Example 3> Skin soothing effect

1) Experimental method

5% each of lactic acid, which is an irritant, was added to the cosmetic compositions of Preparation Examples 1 to 17 and Comparative Examples 1 to 2 in Table 1, and 30 subjects (male and female aged 20-45 years old) It was applied to the site, and the test site was marked with a size of 2 × 2 cm ² , and then subjective efficacy was evaluated.

As for the evaluation items, as shown in Table 5 below, a questionnaire was conducted by classifying the subjective viewpoints of the subjects in detail, and the numerical score for each item was converted into the number of subjects, and the sample with the lowest average value was evaluated as having a superior sedative effect.

Item Evaluation items minimal stimulus mild stimulus normal stimulus strong stimulus very strong stimulus itching, itching One 2 3 4 5

2) Experiment result

The results for skin soothing are shown in Table 6 below.

Sample name usability evaluation medium(%) minimal stimulus mild stimulus normal stimulus strong stimulus very strong stimulus Comparative Example 1 0 0 14 16 0 70.67 Comparative Example 2 0 0 6 19 5 79.33 Preparation Example 1 14 16 0 0 0 30.67 Preparation 2 10 15 One 0 0 28.67 Preparation 3 9 18 3 0 0 36.00 Preparation 4 8 20 2 0 0 36.00 Preparation 5 9 20 One 0 0 34.67 Preparation 6 13 14 3 0 0 33.33 Preparation 7 9 19 2 0 0 35.33 Preparation 8 7 20 3 0 0 37.33 Preparation 9 6 23 One 0 0 36.67 Preparation 10 8 19 3 0 0 36.67 Preparation 11 10 17 3 0 0 35.33 Preparation 12 9 19 2 0 0 35.33 Preparation 13 10 17 3 0 0 35.33 Preparation 14 9 19 2 0 0 35.33 Preparation 15 7 18 5 0 0 38.67 Preparation 16 8 17 5 0 0 38.00 Preparation 17 27 3 0 0 0 22.00

As shown in the results of Table 6, it was confirmed that in all of Preparation Examples 1 to 17, skin irritation was more rapidly alleviated than the cosmetics of Comparative Examples 1 and 2.

Among them, the above kelp extract, green seaweed extract, auditory extract, seaweed extract, green tea extract, Ecklonia cava extract, Japanese radish extract, mother and child extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf mother and child extract, chlorella fermented extract, It can be seen that in the case of Preparation Example 17, in which water, a hydrolyzed sea buckthorn extract, and a hydrolyzed flaxseed green seaweed, were mixed, it showed the most excellent skin soothing effect than Preparations 1 to 16, and thus it was found to be useful as a cosmetic composition.

< test example 4 > Check the free radical scavenging effect

1) Experimental method

The effect on free radical scavenging was confirmed by using the extracted dry powders of Tables Examples 1 to 17. In order to confirm the effect on free radical scavenging, the experiment was conducted as follows.

Experiments were performed using the DPPH (1,1-diphenyl-2 picryl-hydrazyl) method (see: Blois. MSNature 181, 1190, 1958), and DPPH and Quercetin were used by SIGMA. . 2 ml each of Examples 1 to 17 and positive controls of various concentrations (5, 10, 20, 30 ppm) were added to 1 ml of 0.2 mM DPPH methanol solution, stirred, and reacted for 10 minutes at room temperature. Then, absorbance was measured at 517 nm, and purified water was used instead of each sample as a blank test.

The change of free radicals was measured using Equation 1 below, and the results are shown in Table 6.

2) Experiment result

The results for free radical scavenging are shown in Table 7 below.

Sample name Free radical scavenging effect (%) by treatment concentration (mg/ml) SC ₅₀ 0 5 10 20 50 Control (Qurecetin) 0 27.68 67.49 92.48 - 9.41 Example 1 0 21.55 49.37 66.32 93.06 19.33 Example 2 0 22.37 51.13 70.18 93.47 18.52 Example 3 0 20.39 39.39 58.44 88.14 22.38 Example 4 0 21.46 48.22 65.75 92.35 19.65 Example 5 0 21.47 46.35 62.23 91.26 20.47 Example 6 0 21.03 40.02 59.07 88.76 22.05 Example 7 0 20.39 42.45 59.65 89.47 21.66 Example 8 0 19.86 43.34 60.22 90.08 21.42 Example 9 0 20.89 41.38 59.37 89.26 21.78 Example 10 0 20.71 42.03 59.61 89.31 21.69 Example 11 0 20.88 40.64 59.11 89.15 21.92 Example 12 0 20.17 43.91 60.71 90.43 21.20 Example 13 0 19.96 44.18 60.98 90.97 21.06 Example 14 0 20.16 42.47 59.86 89.97 21.57 Example 15 0 20.67 40.41 58.72 89.08 22.03 Example 16 0 19.32 41.36 59.12 89.23 21.98 Example 17 0 27.69 67.73 93.54 - 9.93

As shown in Table 7, it was confirmed that all of Examples 1 to 17 were effective in scavenging free radicals.

In particular, in Example 17, it was confirmed that the antioxidant effect was significantly superior to that of Examples 1 to 16, and it was confirmed that the effect was more excellent with a slight difference compared to the positive control, Quercetin.

Therefore, it was confirmed that the seaweed mixture showed a greater synergistic effect when combined than the individual efficacy, and it was also confirmed that the antioxidant effect was very good.

<Test Example 5> Confirmation of nitric oxide (NO) production inhibitory effect

1) Experimental method

With respect to the extract, RAW 264.7 cells were suspended in an animal cell culture medium containing 10% FBS without phenol red using the dry extraction powder of Examples 1 to 17, and 5 × 10 ⁵ cells per well of a 24-well plate and incubate for 24 hours at 37°C and 5% CO2 incubation conditions. RAW 264.7 cells were simultaneously treated with lipopolysaccharide (LPS) and plant extract or dimethyl sulfoxide (DMSO) and incubated for 20 hours. Then, 100 μl of the supernatant was reacted with 180 μl of Griess reagent. Measure absorbance at 540 nm.

The inhibition rate of nitric oxide was measured according to the calculation method of Equation 2 below, and the results are shown in Table 7.

2) Experiment result

The results for the inhibition of nitric oxide are shown in Table 8 below.

Sample name Lipopolysaccharide inhibition rate (%) by treatment concentration (μg/ml) - + + + + + 0 0 10 50 100 100 untreated group 3.2±2.5 - - - - - LPS sole - 36.7±2.4 - - - - Example 1 - - 30.6 ± 1.6 24.4 ± 2.5 17.4 ± 3.5 13.4 ± 0.5 Example 2 - - 30.2 ± 1.4 24.0 ± 3.3 17.0 ± 1.3 12.8 ± 1.2 Example 3 - - 32.7 ± 2.1 26.2 ± 1.1 19.2 ± 1.1 15.1 ± 2.2 Example 4 - - 31.2 ± 1.7 25.6 ± 1.5 18.6 ± 1.5 14.6 ± 1.2 Example 5 - - 31.6 ± 2.7 25.7 ± 2.7 18.7 ± 1.7 14.6 ± 1.5 Example 6 - - 32.3 ± 1.5 26.2 ± 2.3 19.2 ± 2.3 15.0 ± 1.3 Example 7 - - 32.5 ± 2.2 26.5 ± 1.3 19.5 ± 2.3 15.2 ± 2.2 Example 8 - - 32.0 ± 2.2 25.8 ± 1.2 18.8 ± 1.2 15.0 ± 1.2 Example 9 - - 32.8 ± 1.5 26.5 ± 1.4 19.5 ± 1.4 15.3 ± 1.3 Example 10 - - 32.8 ± 1.3 26.9 ± 2.3 19.9 ± 3.3 15.7 ± 1.3 Example 11 - - 32.2 ± 2.4 25.7 ± 1.5 18.7 ± 1.5 14.9 ± 0.9 Example 12 - - 31.8 ± 2.3 25.6 ± 0.8 18.6 ± 1.5 14.3 ± 0.5 Example 13 - - 31.7 ± 1.3 25.8 ± 1.4 18.8 ± 1.4 14.9 ± 1.2 Example 14 - - 32.3 ± 3.2 26.5 ± 2.1 19.5 ± 1.4 15.6 ± 1.3 Example 15 - - 32.9 ± 2.6 27.2 ± 1.5 20.2 ± 1.5 16.3 ± 2.2 Example 16 - - 32.6 ± 1.4 27.1 ± 1.7 20.1 ± 1.5 16.2 ± 1.3 Example 17 - - 28.7 ± 1.3 20.5 ± 1.6 11.9 ± 1.8 8.7 ± 1.6

As shown in Table 8, it was confirmed that the concentration-dependent inhibition of nitric oxide induced by Examples 1 to 17 lipopolysaccharide.

In particular, in Example 17, compared to Examples 1 to 16, it was confirmed that the antioxidant activity was superior, so it was confirmed that the mixture was more effective in inhibiting inflammation than the effect on each of the seaweeds.

< test example 5 > anti-pollution check the effect

1) Experimental method

In order to confirm the anti-pollution effect of protecting the skin from air pollutants such as benzopyrene, ammonium nitrate, and formaldehyde, an anti-pollution effect confirmation experiment was conducted for Examples 1 to 17. The test was conducted according to a known procedure.

Human keratinocytes HaCaT at a concentration of 5 X 10 ³ cells/ml were dispensed in a 96-well plate and incubated for 24 hours, respectively, benzopyrene (10 μM), ammonium nitrate (500 μg/ml), and formaldehyde (1.5 μg/ml). Examples 1 to 17 were treated and further incubated for 45 hours. After that, MTT solution of 5 mg/ml concentration was added and cultured for 3 hours. Then, the medium was removed, 150ul of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570nm. For each contaminant, the anti-pollution effect was inferred through the degree of cell proliferation.

Anti-pollution effect on benzopyrene (10 μM) (cell proliferation rate (%) of HaCaT) Anti-pollution effect on ammonium nitrate (500 μg/ml) (cell proliferation rate (%) of HaCaT) Anti-pollution effect on formaldehyde (1.5 μg/ml) (cell proliferation rate (%) of HaCaT) Example 1 25.73 28.62 22.34 Example 2 27.45 31.33 22.41 Example 3 8.18 11.76 17.9 Example 4 29.33 24.45 33.11 Example 5 13.94 22.35 14.85 Example 6 17.35 13.42 12.12 Example 7 13.35 13.65 14.75 Example 8 13.9 19.34 16.88 Example 9 14.38 17.28 13.24 Example 10 15.47 18.22 17.54 Example 11 17.11 16.42 13.47 Example 12 11.31 20.66 12.1 Example 13 14.66 21.26 11.65 Example 14 12.21 19.46 15.27 Example 15 10.24 15.66 16.97 Example 16 11.34 16.97 13.87 Example 17 44.34 42.25 46.05

As shown in Table 9, it was confirmed that the cell proliferation rate in benzopyrene, ammonium nitrate and formaldehyde was improved by Examples 1 to 17.

In particular, in Example 17, as it was confirmed that the anti-pollution effect was superior to that of Examples 1 to 16, it was confirmed that the function of protecting the skin from pollutants in the air was increased when seaweed was mixed rather than the effect on each seaweed. there was.

Hereinafter, based on the results of the above-described experimental examples, kelp extract, green seaweed extract, auditory extract, seaweed extract, green tea extract, Ecklonia coli extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, cypress extract, mondang A cosmetic composition containing a algae mixture of leaf cap extract, euglena extract, chlorella fermented product, hydrolyzed sea buckthorn extract, and hydrolyzed serrata is presented as follows. However, the composition of the present invention is not limited to the following formulation examples.

< Formulation Example 1> According to the present invention, kelp extract , seaweed extract, seaweed extract, seaweed extract, green tea extract , Ecklonia radix extract, shiitake extract , mother-and- pearl extract, chlorella extract , flat green seaweed extract, cypress extract , mondang leaf cap extract according to the present invention , Euglena extract, chlorella fermented product , hydrolyzed sea buckthorn extract , and a mixture of hydrolyzed serrata

Example 17 kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf mother and child extract, chlorella fermented extract, Table 9 shows a formulation example of a lotion (skin lotion) in a cosmetic containing a mixture of water, hydrolyzed seaweed extract, and hydrolyzed seaweed.

number Raw material content (wt%) One Example 17
kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia mackerel extract, shiitake extract, mother and child extract, chlorella extract, flat green seaweed extract, laver leaf extract, seaweed extract, euglena fermented extract, chlorella , Hydrolyzed Seaweed Extract and Hydrolyzed Seaweed Mixture 1.0 2 glycerin 3.0 3 butylene glycol 2.0 4 propylene glycol 2.0 5 Polyoxyethylene hydrogenated castor oil 1.0 6 ethanol 10.0 7 triethanolamine 0.1 8 antiseptic a very small amount 9 pigment a very small amount 10 Spices a very small amount 11 Purified water a very small amount

<Production method>

After putting No. 2, 3, 4, and 8 into No. 12 in order and stirring to dissolve, No. 5 is heated to about 60℃ to dissolve, No. 10 is put in to dissolve, and then No. 11 is added. Finally, add Nos. 6, 7, and 9, but add No. 1, stir sufficiently, and then ripen.

< Formulation Example 2> Seaweed extract, seaweed extract, seaweed extract, seaweed extract, green tea extract , Ecklonia radix extract , Japanese shiitake extract , mother - and -pearl extract, chlorella extract , kelp extract , seaweed extract, seaweed extract according to the present invention according to the present invention , Euglena extract, chlorella ferment , hydrolyzed algae mixture of seaweed extract, preparation of nutritional lotion containing

Example 17 kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf mother and child extract, chlorella fermented extract, Table 10 shows a formulation example of a nutritional lotion in a cosmetic containing a mixture of water, hydrolyzed seaweed extract, and hydrolyzed seaweed.

number Raw material content (wt%) One Example 17
kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child extract, chlorella extract, flat green seaweed extract, spearmint extract, mondang leaf extract, euglena fermented extract, chlorella , Hydrolyzed seaweed extract and hydrolyzed seaweed mixture 1.0 2 beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan Sesquiolate 0.5 5 liquid paraffin 10.0 6 Sorbitan Stearate 1.0 7 lipophilic monostearate glycerin 0.5 8 stearic acid 1.5 9 Glyceryl Stearate/PEG-400 Stearate 1.0 10 propylene glycol 3.0 11 carboxy polymer 0.1 12 triethanolamine 0.2 13 antiseptic a very small amount 14 pigment a very small amount 15 Spices a very small amount 16 Purified water a very small amount

<Production method>

10, 11, 12, 13, 16 are mixed and stirred while heated to 80 ~ 85 ℃, put into the manufacturing unit, and then the emulsifier works, and 2, 3, 4, 5, 6, 7, 8, 9, 12 is heated to 80 ~ Emulsify after dissolution by heating between 85 ℃. After emulsification is finished, use a stirrer to cool to 50 ℃, add No. 16, cool to 45 ℃, add No. 14, put No. 1 at 35 ℃, cool to 25 ℃, and then ripen.

< Formulation Example 3> Seaweed extract, green seaweed extract , serrata extract , seaweed extract, green tea extract , Ecklonia cava extract , Japanese radish extract, mother and child extract, chlorella extract, flat green seaweed extract , Preparation of nutritious cream containing seaweed mixture of Mongdang leaf cap banyan extract , euglena extract, chlorella ferment , and hydrolyzed sea buckthorn extract

Example 17 kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf mother and child extract, chlorella fermented extract, Table 11 shows a prescription example of a nourishing cream among cosmetics containing a mixture of water, hydrolyzed seaweed extract, and hydrolyzed seaweed.

number Raw material content (wt%) One Example 17
kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child extract, chlorella extract, flat green seaweed extract, spearmint extract, mondang leaf extract, euglena fermented extract, chlorella , Hydrolyzed seaweed extract and hydrolyzed seaweed mixture 1.0 2 stearic acid 2.0 3 cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 polyoxyethylene sorbitan mono nostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 8 wax 1.0 9 liquid paraffin 4.0 10 squalane 4.0 11 Caprylic/Capric Triglyceride 4.0 12 Carboxyvinyl Polymer 0.3 1 3 butylene glycol 5.0 14 glycerin 3.0 15 triethanolamine 0.5 16 Purified water remaining amount

<Production method>

12, 13, 14, and 16 are mixed and stirred, heated to 80 ~ 85 ℃, put into the manufacturing unit, and then the emulsifier works, and 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 is heated to 80 ~ After dissolving by heating to between 85 °C, 15 is added and stirred to put into the manufacturing unit and emulsify. When the emulsification is finished, it is cooled to 35 ℃ while stirring using a stirrer, and then cooled to 25 ℃ by adding No. 1, and then aged.

< Formulation Example 4> According to the present invention according to the present invention, kelp extract , seaweed extract, seaweed extract, seaweed extract, green tea extract , Ecklonia cava extract , Japanese radish extract, mother and child extract , chlorella extract, flat green seaweed extract , Preparation of essence containing algae mixture of mondang leaf cap half extract , euglena extract, chlorella fermented extract , and hydrolyzed sea buckthorn extract

Example 17 kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child half extract, chlorella extract, flat green seaweed extract, rhizome extract, mondang leaf mother and child extract, chlorella fermented extract, Table 12 shows an example of formulation of an essence in a cosmetic containing a mixture of water, hydrolyzed seaweed extract, and hydrolyzed seaweed.

number Raw material content (wt%) One Example 17
kelp extract, green seaweed extract, kelp extract, seaweed extract, green tea extract, Ecklonia cava extract, shiitake extract, mother and child extract, chlorella extract, flat green seaweed extract, spearmint extract, mondang leaf extract, euglena fermented extract, chlorella , Hydrolyzed seaweed extract and hydrolyzed seaweed mixture 1.0 2 sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 dicetyl phosphate 0.4 8 concentrated glycerin 5.0 9 macadamia oil 15.0 10 Carboxyvinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water remaining amount

<Production method>

2, 3, 4, 5 and 6 are homogenized at a constant temperature and are referred to as nonionic amphiphilic lipids. Mix 7, 8, and 14 with the nonionic amphiphilic lipids, but add No. 1 to mix, homogenize at a constant temperature, pass through a microfluidizer, and then slowly add 9 at a constant temperature to homogenize and then micro Pass it through the fluidizer again, and add 10, 11, 12, and 13 to disperse, stabilize and mature.

Although the present invention has been described in conjunction with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent to that by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (4)

10kg of purified water is added to each of kelp, kelp, green seaweed, green seaweed, seaweed, green tea, Ecklonia serrata, turmeric, mother-of-pearl, flat green seaweed, kelp, mondang leaf cap and euglena, 50 to 60℃, 30 to 60KHz for 4 to 8 hours. , Ultrasonic extraction under the conditions of 200 ~ 700 watts, filtered, concentrated under reduced pressure and freeze-dried at 50℃ or less, kelp extract, green seaweed extract, auditory extract, seaweed extract, seaweed extract, Ecklonia cava extract, shiitake extract, mother and child half extract, flat green leaf extract, With spheroidal laver extract, mongdang leaf cap half extract and euglena extract,
1 kg of ethyl alcohol aqueous solution is added to chlorella, ultrasonically extracted under conditions of 50 to 60 ° C, 30 to 60 KHz, and 200 to 700 watts for 4 to 8 hours, filtered, concentrated under reduced pressure at 50 ° C or less, and freeze-dried chlorella extract;
Add 1 kg of purified water to Chlorella, and inoculate saccharomyces cerevisiae into the chlorella extract obtained by ultrasonic extraction under the conditions of 50 ~ 60 ℃, 30 ~ 60 KHz, 200 ~ 700 watt for 4 ~ 8 hours, and then 48 ~ at 30 ~ 35 ℃ Fermented chlorella fermented under conditions of 72 hours, 100 ~ 200rpm, 0.1 ~ 0.5vvm, filtered through filter paper, concentrated under reduced pressure and freeze-dried;
Hydrolyzed sea buckthorn extract and hydrolyzed flat seaweed,
0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 : 0.01 ~ 10.0 weight ratio A cosmetic composition having skin moisturizing, soothing, antioxidant and anti-inflammatory effects containing a seaweed mixture, comprising the mixed mixture.
delete The method of claim 1,
The extract is a seaweed mixture, characterized in that it is extracted using any one or a mixture of two or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and ethylene glycol A cosmetic composition containing skin moisturizing, soothing, antioxidant and anti-inflammatory effects.
The method of claim 1,
The cosmetic composition is a lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact formulations A cosmetic composition having skin moisturizing, soothing, antioxidant and anti-inflammatory effects containing a seaweed mixture, characterized in that it is formulated as

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