KR102146357B1 - Cosmetic composition including as effective ingredient an extract of citrusfortunella microcarpa - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a calamansi extract as an active ingredient. The cosmetic composition according to the present invention contains a calamansi extract as an active ingredient, but in order to maximize the antioxidant efficacy of the calamansi, the calamansi was dried or frozen and extracted with water or an aqueous ethanol solution, In addition to maximizing the antioxidant effect, the anti-inflammatory effect and skin whitening effect can be simultaneously applied to excellent cosmetics.

Description

Cosmetic composition containing calamansi extract as an active ingredient {COSMETIC COMPOSITION INCLUDING AS EFFECTIVE INGREDIENT AN EXTRACT OF CITRUSFORTUNELLA MICROCARPA}

The present invention relates to a cosmetic composition comprising a calamansi extract as an active ingredient.

Calamansi (Citrusfortunella microcarpa) is a fruit belonging to the dicotyledonous plant A. ), hisperidin, etc., in addition to helping the liver to recover and reducing physical fatigue, it is known to have an effective function to relieve lethargy and dizziness caused by diet, and to burn body fat. .

Recently, as the efficacy of calamansi's antioxidant and diet, etc. is known, additional research on this is being actively conducted.

On the other hand, as the quality of life of modern people improved and the interest in people's appearance increased accordingly, the demand for cosmetics increased rapidly. Among them, interest in functional cosmetics with excellent efficacy such as antioxidant, anti-inflammatory and skin whitening was concentrated. have.

Therefore, among the efficacy of Calamansi, a lot of research has been conducted on the field of cosmetics in relation to the antioxidant effect.

Patent Document 1 proposes a technique for confirming the efficacy related to the skin, including the extract for calamansi.

More specifically, the technology disclosed in Patent Document 1, based on the total weight of the total composition, is 0.1% to 10% by weight of the same tea extract, 0.1% to 5% by weight of the same tea fermentation extract, 0.1% to 5% by weight of Wasong extract, Containing as active ingredients 0.1% to 5% by weight of Aldaria leaf extract, 0.1% to 5% by weight of Calamansi extract and 0.1% to 5% by weight of cacao bean extract, the same tea fermented extract is Water and sugar were added in a weight ratio of 1:1 to 10:0.1 to 0.2, and inoculated with Saccharomyces cerevisiae ATCC 18824, a fermenting microorganism, in an amount of 100,000 cfu/L to 10,000,000 cfu/L. , It discloses a cosmetic composition for whitening and wrinkle improvement using the same, characterized in that by fermentation for 12 to 60 hours at 20 ℃ to 40 ℃.

However, in Patent Document 1, rather than maximizing the unique effect of calamansi, it is introduced as a synergistic component for the antioxidant effect of the tea extract, and the effect of the original calamansi is not sufficiently exhibited, so that the antioxidant effect is insufficient.

In addition, the isobaric extract in Patent Literature 1 is a fermented extract that can be prepared through a process of fermenting Saccharomyces cerevisiae ATCC 18824, a fermenting microorganism. It has a problem that is difficult to mass-produce from the side.

Patent Document 1: Korean Patent Publication No. 10-1875188

The present invention, in order to solve the problems of the prior art, is to provide a cosmetic composition that exhibits the antioxidant effect of Kalamansi and at the same time has an anti-inflammatory effect and an excellent skin whitening effect.

The present invention provides a cosmetic composition comprising a calamansi extract as an active ingredient in order to solve the above-mentioned problems.

More specifically, the present invention comprises a calamansi (Citrusfortunella microcarpa) extract as an active ingredient, and the calamansi (Citrusfortunella microcarpa) extract is dried or frozen calamansi (Citrusfortunella microcarpa) as an extraction solution for 24 hours It provides a cosmetic composition, characterized in that extracted twice during.

In addition, the present invention provides a cosmetic composition, characterized in that the cosmetic composition comprises 0.01% to 20% by weight of the calamansi extract based on the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition, characterized in that the extraction solution is an aqueous solution of water or ethanol.

In addition, the present invention provides a cosmetic composition, characterized in that the ethanol aqueous solution is an ethanol 30% to 99% aqueous solution.

In addition, the present invention provides a cosmetic composition, characterized in that the cosmetic composition is used for anti-aging or anti-inflammatory applications.

In addition, the present invention provides a cosmetic composition, characterized in that the cosmetic composition is used for skin whitening.

In addition, the present invention, the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nutrition cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, CC Cream, soap, body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner, or a cosmetic composition characterized in that it is formulated with at least one selected from the group consisting of conditioners.

In addition, in the present invention, the cosmetic composition is a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic type Or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles. It provides a cosmetic composition characterized in that the.

The cosmetic composition according to the present invention contains a calamansi extract as an active ingredient, but in order to maximize the antioxidant efficacy of the calamansi, the calamansi was dried or frozen and extracted with water or an aqueous ethanol solution, In addition to maximizing the antioxidant effect, the anti-inflammatory effect and skin whitening effect can be simultaneously applied to excellent cosmetics.

The accompanying drawings are for explaining the contents of the present invention in more detail to those skilled in the art, and the technical idea of the present invention is not limited thereto.
1 is a graph showing the result of measuring the total phenol content in Experimental Example 1 according to the present invention.
2 is a diagram showing the results of DPPH radical scavenging activity of calamansi juice in Experimental Example 2 according to the present invention.
3 is a diagram showing the results of DPPH radical scavenging activity of water and ethanol extracts of frozen or dried Kalamansi in Experimental Example 2 according to the present invention.
Figure 4 is a graph showing the measurement result of the nitrite scavenging ability of the calamansi juice juice in Experimental Example 3 according to the present invention.
5 is a graph showing the results of measuring the nitrite scavenging ability of the water and ethanol extracts of frozen or dried Kalamansi in Experimental Example 3 according to the present invention.
6 is a graph showing the result of measuring superoxide radical scavenging ability of calamansi juice juice in Experimental Example 4 according to the present invention.
7 is a graph showing the results of measuring superoxide radical scavenging ability of water and ethanol extracts of frozen or dried Kalamansi in Experimental Example 4 according to the present invention.
8 is a graph showing the results of measuring cell viability (cell toxicity evaluation) of frozen or dried Kalamansi water and ethanol extracts in Experimental Example 5 according to the present invention.
9 is a graph showing the results of the evaluation of inhibition of production of nitrogen monoxide (NO) (evaluation of anti-inflammatory effects) of the water and ethanol extracts of frozen or dried Kalamansi in Experimental Example 6 according to the present invention.
10 is a graph showing the results of L-DOPA oxidation inhibitory ability (skin whitening effect evaluation) of the water and ethanol extracts of frozen or dried Kalamansi in Experimental Example 7 according to the present invention.

Hereinafter, the cosmetic composition according to the present invention will be described in detail, but the scope of the cosmetic composition is not limited by the following description.

The present invention relates to a cosmetic composition.

More specifically, the present invention relates to a cosmetic composition comprising a calamansi (Citrusfortunella microcarpa) extract as an active ingredient.

The calamansi (Citrusfortunella microcarpa) extract is not particularly limited, but is preferably dried or frozen calamansi, for example.

When the calamansi is used as it is without a separate drying or freezing process, it may be difficult to extract the ingredients necessary for exhibiting the efficacy according to the present invention in the calamansi extraction process.

The drying process is not particularly limited, but may be performed by purchasing, for example, Citrusfortunella microcarpa, removing the skin, washing with purified water, and completely drying at 60°C.

In addition, the freezing process is not particularly limited, but for example, it may be carried out by purchasing Citrusfortunella microcarpa, removing the skin, washing with purified water, and freezing it by freezing it at -10°C. have.

The calamansi extract may be extracted twice for 24 hours with an extraction solution, and in this case, it is preferable to use water or an aqueous ethanol solution as the extraction solution.

When an aqueous ethanol solution is used as the extraction solution, it may be 30% to 99%, 40% to 80%, 50% to 80%, or 60% to 80%, and preferably, an aqueous ethanol solution of 70% is preferably used. Do.

When the extraction process and the extraction solution are performed or used differently from those described above, the component of Calamansi may change itself, so that the original function may not be possible and may not be extracted as an extract.

The cosmetic composition may contain 0.01% to 20% by weight of calamansi extract based on the total weight of the cosmetic composition.

In this case, the content range of the calamansi extract may be, for example, 0.01% by weight to 20% by weight, 0.1% by weight to 15% by weight, or 1% by weight to 10% by weight, and If included, the effect of the extract may be insufficient, or the viscosity of the liquid may increase during the extraction process, which may cause problems during filtration.

As described above, the cosmetic composition according to the present invention can be used for anti-aging or anti-inflammatory purposes while having an antioxidant effect by including a calamansi extract as an active ingredient.

Specific experiments and experimental results for use in the anti-aging or anti-inflammatory applications are described in Experimental Examples to be described later.

In addition, the cosmetic composition according to the present invention can be used for skin whitening.

Likewise, specific experiments and experimental results for use in skin whitening purposes are described in Experimental Examples to be described later.

The cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solids, dough anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. Therefore, the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nutrition cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, CC cream, soap, It may be formulated as one or more selected from the group consisting of body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner, or conditioner.

In addition, these compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, as a formulation that can be applied to the skin, it may be prepared and used in a composition in the form of an absorbent gel, pad, patch, sponge, and mask pack, but is not limited thereto.

In addition, the cosmetic composition is a fatty substance, an organic solvent, a solubilizing agent, a thickening and gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, ionic or nonionic Emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles.

In addition, the cosmetic composition according to the present invention is not particularly limited in its applied form, and may be applied in a form applied to the skin, for example, or may be applied in a form absorbed into the skin using a microneedle or the like.

Hereinafter, the present invention will be described in more detail through specific experimental examples. However, these experimental examples are for illustrative purposes only, and the scope of the present invention is not limited to these experimental examples.

[Production Example] Preparation of calamansi extract

The calamansi used in this experimental example was purchased after commercially available calamansi, which was not used as it is, but was frozen and dried. For frozen or dried calamansi, commercially available frozen or dried calamansi were purchased and used.

10 times (frozen) or dry (50 times) water or 70% ethanol was added to 100 g of the frozen or dried calamansi, followed by extraction twice for 24 hours, and the extract was filtered through a filter paper. The filtrate was removed from water or ethanol using a rotary vacuum evaporator (EYELA A-1000S, Tokyo Rikakikai Co., Tokyo, Japan) in a water bath of 37 to 40°C, and freeze-dried to prepare a calamansi extract. Was used as.

The extraction yield of the calaman city for each extraction solvent for each sample is shown in [Table 1] below.

sample extract yield(%) Dry calamansi Water extract 42 70% ethanol extract 50 Frozen calamansi Water extract 8 70% ethanol extract 10

[Experimental Example 1] Measurement of total phenol content

The phenolic compound content was measured using the Folin-Denis method using the principle of showing a blue color by reacting with phosphomolybdic acid, a phenolic substance. 50 μL of 0.5 mg/mL sample solution and 50 μL of Folin-Ciocalteu's phenol reagent (Sigma-Aldrich Co., St. Louis, MO, USA) were mixed and subjected to a dark reaction at room temperature for 3 minutes, followed by 10% sodium carbonate (Na ₂ CO _{3 ).} , Duksan Pure Chemical Co., Ltd.) 750 μL of a solution was added and left for 1 hour in a dark room, and the absorbance of the supernatant was measured at 765 nm. At this time, the content of phenolic compounds was calculated as mg gallic acid per 1 g of sample by creating a standard calibration curve using gallic acid (Sigma-Aldrich Co.) as a standard material and then substituting it.

The measurement results of the total phenol content are shown in [Table 2] below, which is shown in FIG. 1 as a graph.

division Total phenol contents (GAE mg/g DW) Calamansi juice 11.1±1.81 Frozen calamansi Water extract 29.55±4.33 Ethanol extract 15.33±2.70 Dry calamansi Water extract 39.30±6.36 Ethanol extract 28.10±3.55

[Experimental Example 2] DPPH radical scavenging activity

DPPH radical (1,1-diphenyl-2-picrylhydrazyl) scavenging activity was evaluated using the Blois method. A 0.2mM DPPH solution was added to the sample prepared at a constant concentration at 1:1, vortex mixing, and dark reaction at room temperature for 30 minutes, and the absorbance was measured at 517 nm using a spectrophotometer to determine the decrease in absorbance due to reduction of DPPH. At this time, the IC50 value (mg/mL) represents the concentration of the sample that reduces the value of the control group by 50%, in which the solvent used to dissolve the sample instead of the sample is added, and ascorbic acid (Sigma-Aldrich Co.) is used as a positive control for comparison. I did. DPPH radical scavenging activity was calculated by the following equation.

DPPH scavenging activity(%) = {1-(absorbance of sample/absorbance of control)} × 100

The DPPH radical scavenging activity result of the Kalamansi juice is shown in Table 3 below, and is shown in FIG. 2 as a graph.

density(%) Inhibition rate (mean ± standard deviation) 0.025 5±5.1 0.05 12±0.6 0.1 34±0.8 0.5 68±1.3 One 96±1.4 2.5 101±2.2 ※ unit : %

In addition, the DPPH radical scavenging activity results (inhibition rate) of the water and ethanol extracts of the frozen or dried Kalamansi are shown in Table 4 below, which is shown in FIG. 3 as a graph.

density
(mg/mL) Frozen calamansi Dry calamansi Water extract 70% ethanol extract Water extract 70% ethanol extract 0.05 14.26±0.79 46.18±0.39 16.75±1.44 16.75±1.55 0.125 16.16±2.57 51.55±0.15 15.35±0.92 15.35±0.44 0.25 31.93±1.66 59.83±2.23 32.33±0.39 32.33±0.87 0.5 45.80±0.22 83.83±7.74 41.60±4.42 41.60±2.32 One 60.49±0.30 88.12±5.49 59.81±1.21 57.53±1.86 2.5 104.16±1.49 100.97±1.26 114.44±8.40 114.44±0.48 5 148.29±2.58 105.76±1.11 179.05±27.43 179.05±0.58 ※ unit : %

[Experimental Example 3] Measurement of nitrite scavenging ability

Nitrite scavenging ability was measured by Gray and Dugan's method. Add 1 mL of each extract of 2.5, 5, 10 mg/mL to 1 mL of 1 mM NaNO ₂ solution, adjust the pH of the reaction solution to 1.2 with 0.1N HCl (pH 1.2), make the total amount to 10 mL, and react at 37°C for 1 hour. Made it. Griess x reagent (1% sulfanilic acid: 1% naphthylamine = 1:1) was prepared with 5 mL of 2% acetic acid solution and 30% acetic acid. The prepared Griess reagent was added by 0.4 mL to 1 mL of each reaction solution and mixed well. After allowing this mixture to stand at room temperature for 15 minutes, absorbance was measured at 520 nm to measure the amount of nitrous acid remaining. The blank test was carried out in the same manner by adding 0.4 mL of distilled water instead of the Griess reagent. The nitrite scavenging activity was expressed as a percentage with and without adding a sample.

The measurement result of the nitrite scavenging ability of the calamansi juice is shown in Table 5 below, and is shown in FIG. 4 as a graph.

density(%) Inhibition rate (mean ± standard deviation) 0.5 4.84±0.88 One 8.41±0.53 2.5 18.65±1.61 5 31.80±0.90 10 49.03±0.04 20 94.59±0.01 ※ unit : %

In addition, the measurement results (inhibition rate) of nitrite scavenging ability of the frozen or dried Kalamansi water and ethanol extracts are shown in Table 6 below, and are shown in FIG. 5 as a graph.

density
(mg/mL) Frozen calamansi Dry calamansi Water extract 70% ethanol extract Water extract 70% ethanol extract 0.25 - 5.07±0.42 - 7.33±1.70 0.5 0.00±0.98 4.54±0.64 14.63±0.38 12.17±0.34 One 1.94±0.63 9.57±0.26 17.09±0.26 15.82±0.23 2.5 8.54±0.42 24.65±0.87 24.46±0.08 26.77±0.11 5 20.72±1.32 33.27±0.64 36.60±1.02 40.00±0.11 12.5 33.27±0.64 62.34±0.08 65.10±0.42 70.81±0.76 25 66.69±0.00 85.48±0.30 89.68±1.93 92.33±0.34 ※ unit : %

[Experimental Example 4] Superoxide radical scavenging ability measurement

In general, superoxide radicals are generated by xanthine oxidase in vitro. Superoxide radical scavenging activity was measured using the NBT (nitro-blue tetrazolium) reduction method (Parejo et al., 2002). That is, 0.5 mL of 0.1mM phosphate buffer (pH 8.0) containing 0.8mM xanthine and 0.1mM phosphate buffer (pH 8.0) containing 0.48 mM NBT were treated with the extracts at respective concentrations to make a mixture, and the mixture was 37℃ After reacting for 20 minutes, 2.0 mL of 69 mM SDS was added to terminate the reaction, and the absorbance was measured at 560 nm. The concentration of the extract that reduces the inhibitory ability against NBT reduction by 50% according to the amount of sample added by concentration was expressed as (IC50, 50% inhibition concentration).

The measurement result of the superoxide radical scavenging ability of the calamansi juice is shown in Table 7 below, and is shown in FIG. 6 as a graph.

density(%) Scavenging power (mean ± standard deviation) 0.01 7.73±0.51 0.05 14.17±2.65 0.1 20.42±0.11 0.5 50.70±0.60 One 74.93±0.62 5 85.63±1.37 10 104.00±0.17 ※ unit : %

In addition, the measurement results (inhibition rate) of nitrite scavenging ability of the water and ethanol extracts of the frozen or dried Kalamansi are shown in Table 8 below, which is shown in FIG. 7 as a graph.

density
(mg/mL) Frozen calamansi Dry calamansi Water extract 70% ethanol extract Water extract 70% ethanol extract 0.5 - 13.42±0.35 4.90±1.29 2.94±0.55 One 2.13±0.65 16.39±0.47 8.86±0.96 6.34±0.61 2.5 12.83±0.45 22.05±1.14 20.73±0.05 8.70±0.49 5 30.23±0.28 34.64±0.66 40.81±0.13 12.64±0.29 12.5 63.77±0.61 48.37±1.98 72.96±0.3 21.60±0.80 25 101.96±0.74 80.03±1.27 98.52±1.66 61.81±0.89 50 112.92±2.62 92.14±0.58 90.76±4.32 98.38±0.04 ※ unit : %

[Experimental Example 5] Cell viability measurement (cytotoxicity evaluation)

The experiment to measure the cell viability was performed using a water soluble tetrazolium salt-1 (WST-1) assay method. RAW264.7 cells were dispensed in a 96-well plate at a concentration of 1.5 × 10 ⁵ cells/well, cultured for 24 hours, and then the existing medium was removed, and 1ug/ml LPS and samples (0.1, 0.5, 1, 2.5, 5mg/ml) Concentration) was replaced with a medium treated at the same time and cultured for 24 hours. After cell culture, WST-1 solution was cultured at 37° C. for 30 minutes by adding 10% volume of medium per well, and absorbance was measured at 450 nm using a Microplate reader.

The cell viability measurement (cytotoxicity evaluation, viability) results of the frozen or dried calamansi water and ethanol extracts are shown in Table 9 below, and are shown in FIG. 8 as a graph.

division Concentrate (mg/mL) Experimental group 0.1 0.5 One 2.5 5 DW 94.6 92.4 80.9 54.7 47.7 DE 88.0 86.1 81.0 59.5 46.4 FW 92.4 84.0 80.5 64.1 52.8 FE 88.1 88.2 70.6 51.6 26.8 ※ Based on 100 control groups
※ The measured value is rounded to the second decimal place.
※ unit : %

[Experimental Example 6] Nitrogen monoxide (NO) production inhibition evaluation (anti-inflammatory effect evaluation)

RAW264.7 cells were dispensed into a 96-well plate at a concentration of 1.5 × 10 ⁵ cells/well, cultured for 24 hours, and then the existing medium was removed, and 0.05 based on the final concentration of 1 mg/ml of the sample showing a survival rate of 80% or more. , 0.1, 0.25, 0.5, 1mg/ml was replaced with a medium containing the concentration and cultured for 24 hours. The amount of NO produced was measured in the form of NO ₂ ^- present in the cell culture medium using a griess reagent. 100uL of the cell culture supernatant and 100uL of griess reagent were mixed and reacted at room temperature for 10 minutes, and the absorbance was measured at 540 nm, and the NO concentration of the culture was compared with a standard of NaNO ₂ .

The results of the evaluation of inhibition of nitrogen monoxide (NO) production (NO production amount) of the water and ethanol extracts of the frozen or dried calamansi are shown in Table 10 below, and are shown in FIG. 9 as a graph.

division Concentrate (mg/mL) Experimental group 0.05 0.1 0.25 0.5 One DW 25.3 26.3 25.3 20.7 10.7 DE 25.0 27.2 25.9 22.7 15.1 FW 25.1 25.8 24.8 20.8 10.5 FE 24.5 24.9 23.5 21.2 13.0 ※ When treated with LPS as a control, the measured value is 26.8.
※ The measured value is rounded to the second decimal place.
※ Unit: uM

[Experimental Example 7] L-DOPA oxidation inhibitory ability (evaluation of skin whitening effect)

To the mixture of 0.175M sodium phosphate buffer (pH 6.8), 0.2 ml of 10 mM L-DOPA and 0.1 ml of sample solution, 0.2 ml of mushroom tyrosinase (110 U/ml) was added and reacted at 37°C for 10 minutes. Chrome was measured at a wavelength of 475 nm. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance between the addition and no sample solution.

The results of the L-DOPA oxidation inhibitory ability (evaluation of skin whitening effect) of the water and ethanol extracts of the frozen or dried calamansi are shown in Table 11 below, which is shown in FIG. 10 as a graph.

division Concentrate (mg/mL) Experimental group 0.1 0.5 One 2 5 DW 0.4 8.4 17.4 45.7 82.8 DE 0 0.4 9.4 38.0 79.8 FW 0 2.0 6.2 36.3 80.5 FE 1.7 7.2 19.3 44.9 84.5 ※ The measured value is rounded to the second decimal place.
※ unit : %

As can be seen in [Table 1] to [Table 11] and FIGS. 1 to 10, the cosmetic composition comprising the calamansi extract according to the present invention as an active ingredient contains the calamansi extract as an active ingredient However, in order to maximize the antioxidant effect of the calamansi, the calamansi was dried or frozen and extracted with water or an aqueous ethanol solution, and in addition to maximizing the antioxidant effect, the anti-inflammatory effect and skin whitening effect are also used in excellent cosmetics. It can be usefully applied.

The foregoing description of the present application is for illustrative purposes only, and those of ordinary skill in the art to which the present application pertains will be able to understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present application is indicated by the claims to be described later rather than the detailed description, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present application.

Claims (8)

It contains calamansi (Citrusfortunella microcarpa) extract as an active ingredient,
The calamansi (Citrusfortunella microcarpa) extract was removed from the skin and washed with purified water, and then completely dried at 60 ℃ calamansi (Citrusfortunella microcarpa) 70% ethanol aqueous solution was extracted twice for 24 hours with an extraction solution. The yield is 50%,
The calamansi extract is included in 1% to 10% by weight based on the total weight of the cosmetic composition,
0.5 mL of 0.1mM phosphate buffer (pH 8.0) containing 0.8mM xanthine and 0.1mM phosphate buffer (pH 8.0) containing 0.48 mM NBT were treated with the extract in the above concentration range to prepare a mixture, and the mixture was 37°C A cosmetic composition, characterized in that the superoxide radical scavenging ability of the cosmetic composition measured by measuring the absorbance at 560 nm after reaction was terminated by adding 2.0 mL of 69 mM SDS for 20 minutes at, 70 to 110 or less. Composition.
delete delete delete The cosmetic composition according to claim 1, wherein the cosmetic composition is used for anti-aging or anti-inflammatory applications.
The cosmetic composition according to claim 1, wherein the cosmetic composition is used for skin whitening.
The method of claim 1, wherein the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nourishing cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, CC Cream, soap, body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner, or a cosmetic composition, characterized in that formulated with one or more selected from the group consisting of conditioners.
The method of claim 1, wherein the cosmetic composition is a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic agent. Or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles. Cosmetic composition characterized in that the.

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