CN107874261A - The preparation method of one Polysaccharides From Laminaria Japonica health food - Google Patents
The preparation method of one Polysaccharides From Laminaria Japonica health food Download PDFInfo
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- CN107874261A CN107874261A CN201711335801.3A CN201711335801A CN107874261A CN 107874261 A CN107874261 A CN 107874261A CN 201711335801 A CN201711335801 A CN 201711335801A CN 107874261 A CN107874261 A CN 107874261A
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 26
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 25
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 241000015177 Saccharina japonica Species 0.000 title claims abstract description 17
- 235000013402 health food Nutrition 0.000 title claims abstract description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 241000235017 Zygosaccharomyces Species 0.000 claims abstract description 25
- 235000011121 sodium hydroxide Nutrition 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000000047 product Substances 0.000 claims abstract description 20
- 239000007864 aqueous solution Substances 0.000 claims abstract description 19
- 238000000502 dialysis Methods 0.000 claims abstract description 16
- 238000002386 leaching Methods 0.000 claims abstract description 15
- 238000009629 microbiological culture Methods 0.000 claims abstract description 15
- 238000011081 inoculation Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 8
- 239000013049 sediment Substances 0.000 claims abstract description 8
- 238000007654 immersion Methods 0.000 claims abstract description 5
- 241000235033 Zygosaccharomyces rouxii Species 0.000 claims abstract description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- 238000001914 filtration Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 8
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- 239000006188 syrup Substances 0.000 claims description 7
- 235000020357 syrup Nutrition 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 241000209149 Zea Species 0.000 claims 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229920001543 Laminarin Polymers 0.000 abstract description 13
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 abstract description 12
- 229910052740 iodine Inorganic materials 0.000 abstract description 12
- 239000011630 iodine Substances 0.000 abstract description 12
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 abstract description 10
- 239000005717 Laminarin Substances 0.000 abstract description 10
- 238000000855 fermentation Methods 0.000 abstract description 5
- 230000004151 fermentation Effects 0.000 abstract description 5
- 230000000903 blocking effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 17
- 240000008042 Zea mays Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 235000010443 alginic acid Nutrition 0.000 description 3
- 229920000615 alginic acid Polymers 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- DBTMGCOVALSLOR-VPNXCSTESA-N laminarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1O[C@@H]1[C@@H](O)C(O[C@H]2[C@@H]([C@@H](CO)OC(O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-VPNXCSTESA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000227647 Fucus vesiculosus Species 0.000 description 1
- 241001466452 Laminariaceae Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000737023 Zebrasoma flavescens Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- -1 include algin Chemical class 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the preparation method of a Polysaccharides From Laminaria Japonica health food, its method comprises the following steps：（1）The cutting of dry sea-tangle is blocking, add soak and soak, filter and collect sea-tangle；（2）The sea-tangle that step 1 is obtained crushes, and is extracted using sodium hydrate aqueous solution；（3）The leaching liquor of merging is concentrated under reduced pressure, inoculation Lu Shi Zygosaccharomyces fermentations；It is filtered to remove residue；（4）The filtrate that step 3 is obtained carries out dialysis；（5）The liquid component after dialysis is collected, adds ethanol, collects sediment；And it is evaporated under reduced pressure and obtains finished product；In step 3, used Lu Shi Zygosaccharomyceses（Zygosaccharomycesrouxii）It is that deposit number is 2.1522, is preserved in the strain of China General Microbiological culture presevation administrative center.By the present invention in that with immersion and the combination of fermentation process, the relatively low laminarin of amount of iodine is obtained, so as to extend its purposes.
Description
Technical field
The present invention relates to the preparation method of the preparation of polysaccharide, especially a Polysaccharides From Laminaria Japonica health food.
Background technology
Sea-tangle（Laminaria japonica）Phaeophyceae, Laminariaceae.Sporinite is large-scale, brown, flat belt-like, it is most long can
Up to 20M.Leaflet piece, shank and holdfast, holdfast are in rhizoid shape.Blade is made up of epidermis, cortex and marrow tissue, blade
Sporangium is arranged at bottom, has mucilage cavity, can secrete slip material, holdfast arborizations, to adhere to marine rock.It is grown on
Relatively low marine of water temperature.NORTH CHINA is coastal and Zhejiang, ALONG COASTAL FUJIAN are largely cultivated, and yield ranks first in the world.Kelp Rich is containing brown
Phycocolloid and iodine matter, the raw material of industry such as edible and extraction iodine, algin, mannitol.
Algal polysaccharides are essentially from marine algas such as sea-tangle, deer's tail dish (sargassum fusifome), bulk kelp, yellow tang, bladder-wracks.Algal polysaccharides
Mainly include algin, fucoidin and laminaran.Isolated algin, fucoidin and laminaran from sea-tangle
Crude product is the powder of white yellowish.Purified obtained sodium alginate is white filiform；Fucoidin is milky
Powder；The two is dissolved in water, insoluble in organic solvents such as ethanol, acetone, chloroforms.Laminarin from sea-tangle is conventional sea
One of polysaccharides.
Algal polysaccharides, especially laminarin can stimulate various immunocompetent cells (such as macrophage, T lymphocytes, B
Lymphocyte etc.) break up, be ripe, breeding, the immune system of body is restored and is strengthened.Algal polysaccharides contain sulphur mostly
Acidic group, and antivirus action and 5,042 1 contents are into positive correlation.Puritan filler acetifies the antiviral activity and its sulfuric acid of polysaccharide
Group and its content, the size of molecular weight are relevant.Algal polysaccharides calcium composition (CaSP) energy selective depression virus is thin in host
Duplication and propagation in born of the same parents, and the superfluous compound of the calcium ion formed and sulfate radical are that antiviral effect institute is required in host cell
, CasP can suppress the duplication of a small number of envelope virus.
But contain substantial amounts of iodine in sea-tangle, its content of iodine extracted in obtained laminarin also tend to it is higher, it is unfavorable
In the production marketing of iodine excess endemias and edible.
The content of the invention
In order to overcome deficiency of the prior art, it is an object of the invention to provide a kind of rational technology, the sea of advanced technology
Preparation method with polysaccharide health-care food.
The technical solution adopted for the present invention to solve the technical problems is：The preparation side of one Polysaccharides From Laminaria Japonica health food
Method, it is characterised in that：Described method comprises the following steps：
（1）Dry sea-tangle is cut into the block of 1-2 centimetres of size, adds the soak of 10-15 times of its weight, and take the photograph in 10-20
18-24 hours are soaked under family name's degree, filters and collects sea-tangle, described soak includes 0.5-1.5% acetic acid, 5- by weight
7% ethanol, surplus are water；
（2）The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of the Mare Humorum with 1.0-1.5 times of weight
2-4 hours are extracted at 60 c, are used after filtering water-soluble using 10% sodium hydroxide of the Mare Humorum with 1.0-1.5 times of weight
Liquid extracts 2-4 hours at 60 c, and 10% sodium hydroxide water of the Mare Humorum with 1.0-1.5 times of weight is used after filtering
Solution extracts 2-4 hours at 60 c, merges leaching liquor three times；
（3）The leaching liquor of merging is concentrated to the 10-15% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 1-2.5 ×
106Cfu/mL, add corn syrup and reach percentage by weight 1.5-2.2%, and fermented under 40-45 degrees Celsius 2-4 hours；
It is filtered to remove residue；
（4）The filtrate that step 3 is obtained carries out dialysis using the dialyser of 1000 molecular weight；
（5）The liquid component after dialysis is collected, adds the ethanol of 2.5-3 times of volume, collects sediment；And it is evaporated under reduced pressure and obtains
Finished product；In step 3, used Lu Shi Zygosaccharomyceses（Zygosaccharomycesrouxii）It is that deposit number is
2.1522, it is preserved in the strain of China General Microbiological culture presevation administrative center.
In currently preferred aspect, in step 1, dry sea-tangle is cut into the block of 1.5 centimetres of sizes, add its weight
12 times of soak, and soaked 22 hours under 16 degrees Celsius, filter and collect sea-tangle, described soak wraps by weight
1.2% acetic acid, 6% ethanol are included, surplus is water.
In currently preferred aspect, in step 2, the sea-tangle that step 1 is obtained crushes, and uses Mare Humorum band 1.2
15% sodium hydrate aqueous solution of times weight is extracted 3 hours at 60 c, and 1.2 times of weights of Mare Humorum band are used after filtering
10% sodium hydrate aqueous solution of amount extracts 3 hours at 60 c, is used after filtering using 1.2 times of weight of Mare Humorum band
10% sodium hydrate aqueous solution extracts 3 hours at 60 c, merges leaching liquor three times.
In currently preferred aspect, in step 3, the leaching liquor of merging is concentrated to the 12% of original volume under reduced pressure,
Inoculation Lu Shi Zygosaccharomyceses reach 1.8 × 106Cfu/mL, add corn syrup and reach percentage by weight 1.8%, and taken the photograph 42
Fermented 3 hours under family name's degree；It is filtered to remove residue.
In currently preferred aspect, in step 3, while Lu Shi Zygosaccharomyceses are inoculated with, natrium malicum is additionally added
It is 0.05-0.10% to reach percentage by weight, and it is 0.05-0.10% to add dipotassium hydrogen phosphate to reach percentage by weight.
In currently preferred aspect, in step 3, while Lu Shi Zygosaccharomyceses are inoculated with, natrium malicum is additionally added
It is 0.08% to reach percentage by weight, adds dipotassium hydrogen phosphate and reaches percentage by weight for 0.07%.
In currently preferred aspect, in step 4, the filtrate that step 3 is obtained uses the dialyser of 1000 molecular weight
Carry out dialysis 12 hours.
In currently preferred aspect, in steps of 5, the liquid component after dialysis is collected, add the second of 2.8 times of volumes
Alcohol, collect sediment；And it is evaporated under reduced pressure and obtains finished product.
By the present invention in that with immersion and the combination of fermentation process, the relatively low laminarin of amount of iodine is obtained, so as to expand
Its purposes is opened up.This method rational technology, advanced technology, it can be widely applied in the preparation of kelp polysaccharide food.
Embodiment
Unless additionally illustrate, the Lu Shi Zygosaccharomyceses used in embodiments of the invention
（Zygosaccharomycesrouxii）It is that deposit number is 2.1522, is preserved in China General Microbiological culture presevation management
The strain at center.The strain is China General Microbiological culture presevation administrative center strain for sale.
As a comparison, the Zygosaccharomyces of another strain is also used, its preserving number is 2.1017, belongs to identical with planting,
It is preserved in the strain of China General Microbiological culture presevation administrative center.The strain is China General Microbiological culture presevation management
Center strain for sale.
Embodiment 1
In the present embodiment, come into effect from the dry sea-tangle of double centner.Specifically, the preparation side of a Polysaccharides From Laminaria Japonica health food
Method, described method comprise the following steps：
（1）Dry sea-tangle is cut into the block of 1.5 centimetres of sizes, adds the soak of 12 times of its weight, and under 16 degrees Celsius
Immersion 22 hours, filters and collects sea-tangle, described soak includes 1.2% acetic acid, 6% ethanol by weight, and surplus is
Water；
（2）The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of Mare Humorum 1.2 times of weight of band 60
Extract 3 hours, used after filtering Celsius 60 using 10% sodium hydrate aqueous solution of 1.2 times of weight of Mare Humorum band under degree Celsius
The lower extraction of degree 3 hours, 10% sodium hydrate aqueous solution of 1.2 times of weight of Mare Humorum band is used at 60 c after filtering
Extraction 3 hours, merges leaching liquor three times；
（3）The leaching liquor of merging is concentrated to the 12% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 1.8 × 106
Cfu/mL, add corn syrup and reach percentage by weight 1.8%, and fermented 3 hours under 42 degrees Celsius；It is filtered to remove residue；
（4）The filtrate that step 3 is obtained carries out dialysis 12 hours using the dialyser of 1000 molecular weight；
（5）The liquid component after dialysis is collected, adds the ethanol of 2.8 times of volumes, collects sediment；And it is evaporated under reduced pressure and obtains into
Product, finished product weigh 4.15 kilograms.
Embodiment 2
In the present embodiment, come into effect from the dry sea-tangle of double centner.Specifically, the preparation side of a Polysaccharides From Laminaria Japonica health food
Method, described method comprise the following steps：
（1）Dry sea-tangle is cut into the block of 2 centimetres of sizes, adds the soak of 15 times of its weight, and is soaked under 10 degrees Celsius
Bubble 24 hours, filters and collects sea-tangle, described soak includes 1.5% acetic acid by weight, and 5% ethanol, surplus is water；
（2）The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of Mare Humorum 1.5 times of weight of band 60
Extract 2 hours, used after filtering Celsius 60 using 10% sodium hydrate aqueous solution of 1.5 times of weight of Mare Humorum band under degree Celsius
The lower extraction of degree 2 hours, 10% sodium hydrate aqueous solution of 1.5 times of weight of Mare Humorum band is used at 60 c after filtering
Extraction 2 hours, merges leaching liquor three times；
（3）The leaching liquor of merging is concentrated to the 10% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 2.5 × 106
Cfu/mL, add corn syrup and reach percentage by weight 2.2%, and fermented 2 hours under 45 degrees Celsius；It is filtered to remove residue；
（4）The filtrate that step 3 is obtained carries out dialysis 12 hours using the dialyser of 1000 molecular weight；
（5）The liquid component after dialysis is collected, adds the ethanol of 2.5 times of volumes, collects sediment；And it is evaporated under reduced pressure and obtains into
Product, finished product weigh 4.08 kilograms.
Embodiment 3
In the present embodiment, come into effect from the dry sea-tangle of double centner.Specifically, the preparation side of a Polysaccharides From Laminaria Japonica health food
Method, described method comprise the following steps：
（1）Dry sea-tangle is cut into the block of 1 centimetre of size, adds the soak of 10 times of its weight, and is soaked at 20 degrees celsius
Bubble 18 hours, filters and collects sea-tangle, described soak includes 0.5% acetic acid by weight, and 7% ethanol, surplus is water；
（2）The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of Mare Humorum 1.0 times of weight of band 60
Extract 4 hours, used after filtering Celsius 60 using 10% sodium hydrate aqueous solution of 1.0 times of weight of Mare Humorum band under degree Celsius
The lower extraction of degree 4 hours, 10% sodium hydrate aqueous solution of 1.0 times of weight of Mare Humorum band is used at 60 c after filtering
Extraction 4 hours, merges leaching liquor three times；
（3）The leaching liquor of merging is concentrated to the 15% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 1 × 106
Cfu/mL, add corn syrup and reach percentage by weight 1.5%, and fermented 4 hours under 40 degrees Celsius；It is filtered to remove residue；
（4）The filtrate that step 3 is obtained carries out dialysis hour using the dialyser of 1000 molecular weight；
（5）The liquid component after dialysis is collected, adds the ethanol of 3 times of volumes, collects sediment；And it is evaporated under reduced pressure and obtains into
Product, finished product weigh 4.37 kilograms.
Embodiment 4
The method and embodiment 1 of the present embodiment are substantially similar, and its difference is：In step 3, in inoculation Lu Shi Zygosaccharomyceses
Meanwhile it is 0.08% to be additionally added natrium malicum to reach percentage by weight, adds dipotassium hydrogen phosphate and reaches percentage by weight for 0.07%.
Finished product weighs 4.16 kilograms.
Embodiment 5
The method and embodiment 1 of the present embodiment are substantially similar, and its difference is：In step 3, in inoculation Lu Shi Zygosaccharomyceses
Meanwhile it is 0.10% to be additionally added natrium malicum to reach percentage by weight, adds dipotassium hydrogen phosphate and reaches percentage by weight for 0.10%.
Finished product weighs 4.09 kilograms.
Embodiment 6
The method and embodiment 1 of the present embodiment are substantially similar, and its difference is：In step 3, in inoculation Lu Shi Zygosaccharomyceses
Meanwhile it is 0.05% to be additionally added natrium malicum to reach percentage by weight, adds dipotassium hydrogen phosphate and reaches percentage by weight for 0.05%.
Finished product weighs 4.22 kilograms.
Comparative example 1
The method and embodiment 1 of this comparative example are substantially similar, and its difference is：Used Lu Shi Zygosaccharomyceses are that preserving number is
2.1017, category kind is identical with embodiment 1, is preserved in the strain of China General Microbiological culture presevation administrative center.The bacterium
Kind is China General Microbiological culture presevation administrative center strain for sale.Finished product weighs 3.14 kilograms.
Comparative example 2
The method and embodiment 4 of this comparative example are substantially similar, and its difference is：Used Lu Shi Zygosaccharomyceses are that preserving number is
2.1017, category kind is identical with embodiment 1, is preserved in the strain of China General Microbiological culture presevation administrative center.The bacterium
Kind is China General Microbiological culture presevation administrative center strain for sale.Finished product weighs 3.54 kilograms.
Comparative example 3
The method and embodiment 1 of this comparative example are substantially similar, and its difference is：Used Lu Shi Zygosaccharomyceses are that preserving number is
2.1913, category kind is identical with embodiment 1, is preserved in the strain of China General Microbiological culture presevation administrative center.The bacterium
Kind is China General Microbiological culture presevation administrative center strain for sale.Finished product weighs 3.55 kilograms.
Comparative example 4
The method and embodiment 4 of this comparative example are substantially similar, and its difference is：Used Lu Shi Zygosaccharomyceses are that preserving number is
2.1913, category kind is identical with embodiment 1, is preserved in the strain of China General Microbiological culture presevation administrative center.The bacterium
Kind is China General Microbiological culture presevation administrative center strain for sale.Finished product weighs 3.17 kilograms.
Comparative example 5
The method and embodiment 1 of this comparative example are substantially similar, and its difference is：In step 3, in inoculation Lu Shi Zygosaccharomyceses
Meanwhile it is additionally added dipotassium hydrogen phosphate and reaches percentage by weight for 0.07%.
The content of iodine analysis experiment of laminarin product
In this experiment, the laminarin obtained by embodiment 1-6 and comparative example 1-4 is subjected to content of iodine analysis.The content of iodine
Analyze the method for taking SC/T 3010-2001（Directly measured since the aqueous solution of laminarin, acquisition of not burning
The step of ash content or immersion）.
Measurement result is recorded in table 1.
Table 1：The content of iodine analysis result of laminarin obtained by embodiment 1-6 and comparative example 1-3
| Group number | KI contents（Mg/kg laminarins） |
| Embodiment 1 | 12.4 |
| Embodiment 2 | 13.7 |
| Embodiment 3 | 12.9 |
| Embodiment 4 | 4.4 |
| Embodiment 5 | 5.1 |
| Embodiment 6 | 5.3 |
| Comparative example 1 | 108.9 |
| Comparative example 2 | 114.3 |
| Comparative example 3 | 53.4 |
| Comparative example 4 | 43.6 |
| Comparative example 5 | 12.7 |
It can be seen that, in embodiments of the invention 1-3, the content of iodine for obtaining final products has significantly with KI calculating from table 1
Reduce.By fermentation process, removed from laminarin（Absorb）Substantial amounts of I.During the fermentation add auxiliary agent it
Afterwards, the absorbability of yeast increases.Although also there is certain assimilation effect after strain has been changed, and not as this
Invention is obvious so.Strain selected by the present invention substantially has stronger I absorbability than other strains, brings
Significant effect, and the strain has preferably response for additional auxiliary agent, helps to obtain the sea of lower content of iodine
Band polyose.
Claims (8)
1. the preparation method of a Polysaccharides From Laminaria Japonica health food, it is characterised in that：Described method comprises the following steps：
（1）Dry sea-tangle is cut into the block of 1-2 centimetres of size, adds the soak of 10-15 times of its weight, and take the photograph in 10-20
18-24 hours are soaked under family name's degree, filters and collects sea-tangle, described soak includes 0.5-1.5% acetic acid, 5- by weight
7% ethanol, surplus are water；
（2）The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of the Mare Humorum with 1.0-1.5 times of weight
2-4 hours are extracted at 60 c, are used after filtering water-soluble using 10% sodium hydroxide of the Mare Humorum with 1.0-1.5 times of weight
Liquid extracts 2-4 hours at 60 c, and 10% sodium hydroxide water of the Mare Humorum with 1.0-1.5 times of weight is used after filtering
Solution extracts 2-4 hours at 60 c, merges leaching liquor three times；
（3）The leaching liquor of merging is concentrated to the 10-15% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 1-2.5 ×
106Cfu/mL, add corn syrup and reach percentage by weight 1.5-2.2%, and fermented under 40-45 degrees Celsius 2-4 hours；
It is filtered to remove residue；
（4）The filtrate that step 3 is obtained carries out dialysis using the dialyser of 1000 molecular weight；
（5）The liquid component after dialysis is collected, adds the ethanol of 2.5-3 times of volume, collects sediment；And it is evaporated under reduced pressure and obtains
Finished product；In step 3, used Lu Shi Zygosaccharomyceses（Zygosaccharomycesrouxii）It is that deposit number is
2.1522, it is preserved in the strain of China General Microbiological culture presevation administrative center.
2. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In step 1,
Dry sea-tangle is cut into the block of 1.5 centimetres of sizes, adds the soak of 12 times of its weight, and immersion 22 is small under 16 degrees Celsius
When, filter and collect sea-tangle, described soak includes 1.2% acetic acid by weight, and 6% ethanol, surplus is water.
3. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In step 2,
The sea-tangle that step 1 is obtained crushes, and uses 15% sodium hydrate aqueous solution of Mare Humorum 1.2 times of weight of band at 60 degrees Celsius
Lower extraction 3 hours, use after filtering and soaked at 60 c using 10% sodium hydrate aqueous solution of 1.2 times of weight of Mare Humorum band
Carry 3 hours, to extract 3 at 60 c small for 10% sodium hydrate aqueous solution for using using 1.2 times of weight of Mare Humorum band after filtering
When, merge leaching liquor three times.
4. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In step 3,
The leaching liquor of merging is concentrated to the 12% of original volume under reduced pressure, inoculation Lu Shi Zygosaccharomyceses reach 1.8 × 106Cfu/mL,
Add corn syrup and reach percentage by weight 1.8%, and fermented 3 hours under 42 degrees Celsius；It is filtered to remove residue.
5. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In step 3,
While Lu Shi Zygosaccharomyceses are inoculated with, it is 0.05-0.10% to be additionally added natrium malicum to reach percentage by weight, adds phosphoric acid hydrogen
It is 0.05-0.10% that dipotassium, which reaches percentage by weight,.
6. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 5, it is characterised in that：In step 3,
While Lu Shi Zygosaccharomyceses are inoculated with, it is 0.08% to be additionally added natrium malicum to reach percentage by weight, adds dipotassium hydrogen phosphate and reaches
It is 0.07% to percentage by weight.
7. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In step 4,
The filtrate that step 3 is obtained carries out dialysis 12 hours using the dialyser of 1000 molecular weight.
8. the preparation method of Polysaccharides From Laminaria Japonica health food according to claim 1, it is characterised in that：In steps of 5,
The liquid component after dialysis is collected, adds the ethanol of 2.8 times of volumes, collects sediment；And it is evaporated under reduced pressure and obtains finished product.
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| CN111034994A (en) * | 2019-11-18 | 2020-04-21 | 福建卫生职业技术学院 | Preparation method of functional fermented laminarin |
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