CN107789306A - A kind of Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition and preparation method thereof - Google Patents
A kind of Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The invention discloses a kind of Korean ginseng stem cell extract to moisten preserving moisture and protecting skin composition and preparation method thereof, and the skin care compositions is made up of the component of following parts by weight：20~50 parts of 0.1~3 part of Korean ginseng stem cell extract, 0.3~1 part of Cortex Mori extract, 0.1~1 part of nostoc sphaeroids kutz extractive, 0.5~4 part of adlay extract, 0.5~5 part of white pond lily root extract, 8~15 parts of jojoba oil, 1~3 part of emulsifying agent, 2~4 parts of NMF, 0.3~0.8 part of xanthans and deionized water.Skin care compositions each component synergy of the present invention protrudes, gentle non-stimulated the effect of having good moist moisturizing and improve skin elasticity.
Description
Technical field
The present invention relates to skin care field, and in particular to a kind of Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition
And preparation method thereof.
Background technology
Human body skin is the natural cover for defense for resisting external harmful substance together, however, due to solar ultraviolet, computer spoke
Penetrate, the dust in air, stay up late and the factor such as aging causes the various problems of face skin, dry skin, gradual aging, corium
Layer atrophy water shortage, overstimulation melanocyte, foxiness is caused, the elastic fibers and collagenous fibres of skin, which are degenerated, to be deformed, skin
Following the string becomes loose, wrinkle easily occurs.
Containing chemistry, side effect in varying degrees is inevitably all brought to the skin of people for present cosmetics,
Pure natural plant extract is added in skin care item, directly there is moist, smooth, moisturizing and other effects, easily fully inhaled by skin
Receive.Plant stem cell is undifferentiated cell in plant, is the source and signals-modulating center to grow, is had very strong
Self-renewing and lasting splitting ability, may advantageously facilitate the metabolism of Skin Cell, use it for skin, have and grow well
Moisten skin-care effect.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Korean ginseng stem cell extract to moisten moisturizing
Skin care compositions, there is good moist moisture-keeping efficacy.
It is another object of the present invention to provide a kind of Korean ginseng stem cell extract to moisten preserving moisture and protecting skin composition
Preparation method.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition, is made up of the component of following parts by weight：It is high
Beautiful 0.1~3 part of ginseng stem cell extract, 0.3~1 part of Cortex Mori extract, 0.1~1 part of nostoc sphaeroids kutz extractive, adlay extract
0.5~4 part, it is 0.5~5 part of white pond lily root extract, 8~15 parts of jojoba oil, 1~3 part of emulsifying agent, 2~4 parts of NMF, yellow
20~50 parts of 0.3~0.8 part of virgin rubber and deionized water.
Korean ginseng stem cell extract, which is derived from, in above-mentioned skincare composition has very strong self-renewing and lasting splitting ability
Korean ginseng stem cell, may advantageously facilitate the metabolism of Skin Cell, improve skin elasticity, have and moisten crease-resistant shield well
Skin effect；Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and white pond lily root extract can be supplemented persistently to skin
Water nutrition, have water absorbing capacity by force and the characteristics of moistening effect is good, effectively recovery skin vitality and elastic force；The skincare composition
Thing each component synergy protrudes, and has the effect of good in moisturizing and in terms of improving skin elasticity, and gentle to skin, does not have
Stimulate.
The preferred embodiment of preserving moisture and protecting skin composition is moistened as Korean ginseng stem cell extract of the present invention, by
The component of following parts by weight is made：0.9~1.5 part of Korean ginseng stem cell extract, 0.4~0.7 part of Cortex Mori extract, Pueraria lobota
Celestial 0.5~0.8 part of rice extracts, 1.2~2.5 parts of adlay extract, 1~3 part of white pond lily root extract, jojoba oil 8~15
Part, 1~3 part of emulsifying agent, 2~4 parts of NMF, 0.3~0.8 part of xanthans and 20~50 parts of deionized water.
The preferred embodiment of preserving moisture and protecting skin composition is moistened as Korean ginseng stem cell extract of the present invention, by
The component of following parts by weight is made：1.1 parts of Korean ginseng stem cell extract, 0.5 part of Cortex Mori extract, nostoc sphaeroids kutz extractive
0.7 part, 2 parts of adlay extract, 2.5 parts of white pond lily root extract, 8~15 parts of jojoba oil, 1~3 part of emulsifying agent, NMF 2
20~50 parts of~4 parts, 0.3~0.8 part of xanthans and deionized water.
The preferred embodiment of preserving moisture and protecting skin composition, institute are moistened as Korean ginseng stem cell extract of the present invention
It is Korean ginseng forming layer stem cell to state Korean ginseng stem cell.
The preferred embodiment of preserving moisture and protecting skin composition, institute are moistened as Korean ginseng stem cell extract of the present invention
The Korean ginseng stem cell extract stated includes the stem cell media and the lysate of the stem cell of culture after culture stem cell.
The preferred embodiment of preserving moisture and protecting skin composition, institute are moistened as Korean ginseng stem cell extract of the present invention
NMF is stated as at least one of glycerine, propane diols and sorbierite.
The preferred embodiment of preserving moisture and protecting skin composition, institute are moistened as Korean ginseng stem cell extract of the present invention
It is lanolin, paraffin or glycerin monostearate to state emulsifying agent.
Present invention also offers the Korean ginseng stem cell extract described in any of the above-described to moisten preserving moisture and protecting skin composition
Preparation method, comprise the following steps：
(1) by the stem cell media and the lysate of the stem cell of culture after culture stem cell, obtained after freeze-drying
Freeze-dried powder；
(2) each component raw material is weighed in proportion；
(3) it is xanthans and NMF is soluble in water, stirred at 70~80 DEG C, obtain aqueous phase；
(4) it will be well mixed at 70~80 DEG C of jojoba oil and emulsifying agent, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirred, it is cooled to 40~
After 50 DEG C, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and white pond lily root are added
Extract, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
The preferred of the preparation method of preserving moisture and protecting skin composition is moistened as Korean ginseng stem cell extract of the present invention
Embodiment, the culture medium, through ultrafiltration membrance filter, are carried out dense before freeze-drying using rotatory vacuum concentrating instrument to filtrate
Contracting, remove phenol red in culture medium, the active component in reservation culture medium.
The preferred of the preparation method of preserving moisture and protecting skin composition is moistened as Korean ginseng stem cell extract of the present invention
Embodiment, the aperture of the milipore filter is 200nm.
Compared with prior art, the invention has the advantages that：
The Korean ginseng stem cell extract of the present invention is moistened in preserving moisture and protecting skin composition, and Korean ginseng stem cell extract is derived from
Korean ginseng stem cell with very strong self-renewing and lasting splitting ability, may advantageously facilitate the metabolism of Skin Cell,
Skin elasticity is improved, has and moistens crease-resistant skin-care effect well；Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract
It can persistently be kept the skin wet nutrition to skin with white pond lily root extract, have water absorbing capacity strong and the characteristics of moistening effect is good,
Effectively recover skin vitality and elastic force；The skin care compositions each component synergy protrudes, and in moisturizing and improves skin elasticity
Aspect has the effect of good, and gentle to skin, does not stimulate.
Brief description of the drawings
Fig. 1 is keratoderma moisture determination result.
Fig. 2 is skin elasticity measurement result.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment to the present invention
Further illustrate.It will be appreciated by those skilled in the art that specific embodiment described herein is only to explain the present invention, not
For limiting the present invention.
Test method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
The material that uses, reagent etc., unless otherwise specified, are commercially obtained.
Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and white pond lily root extract are purchased from the green rattan biology in Xi'an
Co., Ltd.
Embodiment 1
Korean ginseng stem cell extract of the present invention moistens a kind of embodiment of the preparation method of preserving moisture and protecting skin composition, this reality
Apply the example Korean ginseng stem cell extract and moisten the preparation method of preserving moisture and protecting skin composition and be：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 200nm ultrafiltration membrance filter, is used
Rotatory vacuum concentrating instrument concentrates to filtrate, removes phenol red in culture medium, the active component in reservation culture medium；Will culture
Base and the freeze-drying of the lysate of Korean ginseng forming layer stem cell, obtain Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1.1 parts of Korean ginseng stem cell extract, 0.5 part of Cortex Mori extract, 0.7 part of nostoc sphaeroids kutz extractive, adlay extraction
2 parts of thing, 2.5 parts of white pond lily root extract, 9 parts of jojoba oil, 1.5 parts of lanolin, 4 parts of glycerine, 0.4 part of xanthans and deionization
45 parts of water.
(3) it is xanthans and glycerine is soluble in water, stirred at 75 DEG C, obtain aqueous phase；
(4) it will be well mixed at 75 DEG C of jojoba oil and lanolin, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to 45 DEG C
Afterwards, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and the extraction of white pond lily root are added
Thing, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
Embodiment 2
Korean ginseng stem cell extract of the present invention moistens a kind of embodiment of the preparation method of preserving moisture and protecting skin composition, this reality
Apply the example Korean ginseng stem cell extract and moisten the preparation method of preserving moisture and protecting skin composition and be：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 200nm ultrafiltration membrance filter, is used
Rotatory vacuum concentrating instrument concentrates to filtrate, removes phenol red in culture medium, the active component in reservation culture medium；Will culture
Base and the freeze-drying of the lysate of Korean ginseng forming layer stem cell, obtain Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
0.1 part of Korean ginseng stem cell extract, 0.3 part of Cortex Mori extract, 0.1 part of nostoc sphaeroids kutz extractive, adlay extraction
0.5 part of thing, 5 parts of white pond lily root extract, 8 parts of jojoba oil, 3 parts of glycerin monostearate, 2 parts of propane diols, 0.3 part of xanthans
With 20 parts of deionized water.
(3) it is xanthans and propane diols is soluble in water, stirred at 70 DEG C, obtain aqueous phase；
(4) it will be well mixed at 70 DEG C of jojoba oil and glycerin monostearate, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to 45 DEG C
Afterwards, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and the extraction of white pond lily root are added
Thing, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
Embodiment 3
Korean ginseng stem cell extract of the present invention moistens a kind of embodiment of the preparation method of preserving moisture and protecting skin composition, this reality
Apply the example Korean ginseng stem cell extract and moisten the preparation method of preserving moisture and protecting skin composition and be：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 200nm ultrafiltration membrance filter, is used
Rotatory vacuum concentrating instrument concentrates to filtrate, removes phenol red in culture medium, the active component in reservation culture medium；Will culture
Base and the freeze-drying of the lysate of Korean ginseng forming layer stem cell, obtain Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
3 parts of Korean ginseng stem cell extract, 1 part of Cortex Mori extract, 1 part of nostoc sphaeroids kutz extractive, 7 parts of adlay extract,
0.5 part of white pond lily root extract, 15 parts of jojoba oil, 1 part of glycerin monostearate, 4 parts of sorbierite, 0.8 part of xanthans and go
50 parts of ionized water.
(3) it is xanthans and NMF is soluble in water, stirred at 80 DEG C, obtain aqueous phase；
(4) it will be well mixed at 80 DEG C of jojoba oil and glycerin monostearate, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to 50 DEG C
Afterwards, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and the extraction of white pond lily root are added
Thing, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
Embodiment 4
Korean ginseng stem cell extract of the present invention moistens a kind of embodiment of the preparation method of preserving moisture and protecting skin composition, this reality
Apply the example Korean ginseng stem cell extract and moisten the preparation method of preserving moisture and protecting skin composition and be：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 200nm ultrafiltration membrance filter, is used
Rotatory vacuum concentrating instrument concentrates to filtrate, removes phenol red in culture medium, the active component in reservation culture medium；Will culture
Base and the freeze-drying of the lysate of Korean ginseng forming layer stem cell, obtain Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
0.9 part of Korean ginseng stem cell extract, 0.7 part of Cortex Mori extract, 0.5 part of nostoc sphaeroids kutz extractive, adlay extraction
2.5 parts of thing, 3 parts of white pond lily root extract, 13 parts of jojoba oil, 2 parts of paraffin, 1 part of glycerine, 1 part of sorbierite, 0.7 part of xanthans
With 45 parts of deionized water.
(3) it is xanthans, glycerine and sorbierite is soluble in water, stirred at 70 DEG C, obtain aqueous phase；
(4) it will be well mixed at 70 DEG C of jojoba oil and paraffin, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to, 50
After DEG C, add Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and white pond lily root and carry
Thing is taken, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
Embodiment 5
Korean ginseng stem cell extract of the present invention moistens a kind of embodiment of the preparation method of preserving moisture and protecting skin composition, this reality
Apply the example Korean ginseng stem cell extract and moisten the preparation method of preserving moisture and protecting skin composition and be：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, via hole diameter is 200nm ultrafiltration membrance filter, is used
Rotatory vacuum concentrating instrument concentrates to filtrate, removes phenol red in culture medium, the active component in reservation culture medium；Will culture
Base and the freeze-drying of the lysate of Korean ginseng forming layer stem cell, obtain Korean ginseng stem cell extract.
(2) each component raw material is weighed by following part by weight
1.5 parts of Korean ginseng stem cell extract, 0.4 part of Cortex Mori extract, 0.8 part of nostoc sphaeroids kutz extractive, adlay extraction
1.2 parts of thing, 1 part of white pond lily root extract, 10 parts of jojoba oil, 1 part of lanolin, 2 parts of glycerine, 2 parts of propane diols, xanthans 0.5
30 parts of part and deionized water.
(3) it is xanthans, glycerine and propane diols is soluble in water, stirred at 75 DEG C, obtain aqueous phase；
(4) it will be well mixed at 75 DEG C of jojoba oil and lanolin, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to 40 DEG C
Afterwards, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and the extraction of white pond lily root are added
Thing, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition efficacy assessments
Using the skin care compositions prepared by embodiment 1~5 as experimental group, with the skincare composition prepared by comparative example 1~3
Thing as a control group, carries out related efficacy test.The extract of comparative example is made up of the component of table 1, other components and preparation
Method is the same as embodiment 1.
Table 1
First, keratoderma moisture determination
Skin care compositions made from embodiment 1~5 and comparative example 1~3, each group selected 30 ages at 35~55 years old respectively
Female volunteers, smearing skin care compositions on the face after volunteer's face cleaning, be used continuously 6 months, pass through skinanalysis apparatus
(MC750, German CK) determines water content of stratum corneum, and test result is as shown in Figure 1.
Have in keratoderma with reference to water and the two kinds of moisture of free water, moisture can influence cuticula reclaimed water and
The formation of grease sebum film, sebum film can prevent moisture excessive evaporation, softening skin, have important meaning to the anti-aging of skin
Justice.As shown in Figure 1, after Korean ginseng stem cell skin care composition of the volunteer using embodiment preparation, keratoderma water content
Be substantially better than comparative example, show Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and
The combination of white pond lily root extract can cooperate with the water content for improving skin, reduce skin water loss, and there is good moisturizing to imitate
Fruit.
2nd, skin elasticity is tested
Skin care compositions made from embodiment 1~5 and comparative example 1~3, every group was selected 30 ages at 35~55 years old respectively
Female volunteers, volunteer is daily early, is smearing skin care compositions on the face after late face cleaning, is used continuously 6 months, is surveyed by skin
Instrument (MC750, German CK) measure skin elasticity is tried, test result is as shown in Figure 2.
The numerical value of coefficient of skin elasticity is bigger, and skin elasticity is better.As shown in Figure 2, volunteer is prepared using embodiment
After Korean ginseng stem cell skin care composition, coefficient of skin elasticity is apparently higher than comparative example, Korean ginseng stem cell extract, paper mulberry bark
Extract, nostoc sphaeroids kutz extractive, adlay extract and white pond lily root extract have good collaboration moisturizing moisturizing effect, are advantageous to
Improve the elasticity of skin.
3rd, human body skin adverse reaction is tested
The female volunteers 180 that the age is 20~50 years old are chosen, are randomly divided into 6 groups, every group of each 30 people.Reference《Make up
Product hygienic practice》The related request of " human skin patch " carries out human body skin adverse reaction experiment in (2007 editions).Point
Korean ginseng stem cell sunlight screening skin-protecting product prepared by 0.025g embodiment 1~5 is not added on spot examination device, and with non-stimulated glue
Spot examination device is attached to the forearm song side of volunteer by band, is gently pressed with hand and is allowed to uniformly be attached on skin, except despeckle tries device after 24h,
Dermoreaction is observed after 30min, 1h, 2h, 24h respectively, and according to table 2《Skin adverse reaction grade scale table》Record is anti-
Answer result.
Table 2
Table 3
| Grading system | Number of cases | Ratio |
| 0 | 30 | 100% |
| 1 | 0 | 0 |
| 2 | 0 | 0 |
| 3 | 0 | 0 |
| 4 | 0 | 0 |
Table 3 is the skin adverse reaction that Korean ginseng stem cell extract prepared by embodiment 1 moistens preserving moisture and protecting skin composition
As a result.Korean ginseng stem cell extract prepared by embodiment 2~5 moistens the skin adverse reaction result of preserving moisture and protecting skin composition
It is same as Example 1.Result of the test shows that volunteer's result of the test of 1~5 group of the embodiment of the present invention is feminine gender, without bad
Dermoreaction occurs, and shows that Korean ginseng stem cell sunlight screening skin-protecting product prepared by the present invention is gently non-stimulated, security is good.
Result above shows that Korean ginseng stem cell extract of the invention, which moistens preserving moisture and protecting skin composition, has good association
With moist moisture-keeping efficacy, skin water loss can be reduced, improves skin elasticity, and it is gentle non-stimulated, security is good.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of Korean ginseng stem cell extract moistens preserving moisture and protecting skin composition, it is characterised in that by the group of following parts by weight
Divide and be made：0.1~3 part of Korean ginseng stem cell extract, 0.3~1 part of Cortex Mori extract, 0.1~1 part of nostoc sphaeroids kutz extractive,
0.5~4 part of adlay extract, 0.5~5 part of white pond lily root extract, 8~15 parts of jojoba oil, 1~3 part of emulsifying agent, NMF
20~50 parts of 2~4 parts, 0.3~0.8 part of xanthans and deionized water.
2. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that by with
The component of lower parts by weight is made：0.9~1.5 part of Korean ginseng stem cell extract, 0.4~0.7 part of Cortex Mori extract, Ge Xian
0.5~0.8 part of rice extracts, 1.2~2.5 parts of adlay extract, 1~3 part of white pond lily root extract, 8~15 parts of jojoba oil,
20~50 parts of 1~3 part of emulsifying agent, 2~4 parts of NMF, 0.3~0.8 part of xanthans and deionized water.
3. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that by with
The component of lower parts by weight is made：1.1 parts of Korean ginseng stem cell extract, 0.5 part of Cortex Mori extract, nostoc sphaeroids kutz extractive
0.7 part, 2 parts of adlay extract, 2.5 parts of white pond lily root extract, 8~15 parts of jojoba oil, 1~3 part of emulsifying agent, NMF 2
20~50 parts of~4 parts, 0.3~0.8 part of xanthans and deionized water.
4. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that described
Korean ginseng stem cell is Korean ginseng forming layer stem cell.
5. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that described
Korean ginseng stem cell extract include culture stem cell after stem cell media and culture stem cell lysate.
6. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that described
NMF is at least one of glycerine, propane diols and sorbierite.
7. Korean ginseng stem cell extract according to claim 1 moistens preserving moisture and protecting skin composition, it is characterised in that described
Emulsifying agent is lanolin, paraffin or glycerin monostearate.
8. the Korean ginseng stem cell extract according to any one of claim 1~7 moistens the preparation of preserving moisture and protecting skin composition
Method, it is characterised in that comprise the following steps：
(1) by the stem cell media and the lysate of the stem cell of culture after culture stem cell, freezed after freeze-drying
Powder；
(2) each component raw material is weighed in proportion；
(3) it is xanthans and NMF is soluble in water, stirred at 70~80 DEG C, obtain aqueous phase；
(4) it will be well mixed at 70~80 DEG C of jojoba oil and emulsifying agent, obtain oil phase；
(5) oil phase prepared by step (4) is added in the aqueous phase of step (3) preparation, stirs, be cooled to 40~50 DEG C
Afterwards, Korean ginseng stem cell extract, Cortex Mori extract, nostoc sphaeroids kutz extractive, adlay extract and the extraction of white pond lily root are added
Thing, abundant homogeneous, is cooled to room temperature, obtains the skin care compositions.
9. Korean ginseng stem cell extract according to claim 8 moistens the preparation method of preserving moisture and protecting skin composition, it is special
Sign is that the culture medium, through ultrafiltration membrance filter, concentrates filtrate before freeze-drying, removes phenol red in culture medium, reservation training
Support the active component in base.
10. Korean ginseng stem cell extract according to claim 9 moistens the preparation method of preserving moisture and protecting skin composition, it is special
Sign is that the aperture of the milipore filter is 200nm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908069A (en) * | 2019-04-24 | 2019-06-21 | 福州东艺善秀商业有限公司 | A kind of milky lotion of multifunctional plant extractive and preparation method thereof |
| US11786451B2 (en) | 2019-06-28 | 2023-10-17 | The Procter & Gamble Company | Skin care composition |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822630A (en) * | 2010-03-24 | 2010-09-08 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroids kutz extractive and application thereof in cosmetics |
| CN102159182A (en) * | 2008-06-13 | 2011-08-17 | 云火公司 | Composition for anti-aging or antioxidation containing plant stem cell lines derived from cambium of panax ginseng including wild ginseng and ginseng as active components |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2017
- 2017-10-31 CN CN201711055086.8A patent/CN107789306A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102159182A (en) * | 2008-06-13 | 2011-08-17 | 云火公司 | Composition for anti-aging or antioxidation containing plant stem cell lines derived from cambium of panax ginseng including wild ginseng and ginseng as active components |
| CN101822630A (en) * | 2010-03-24 | 2010-09-08 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroids kutz extractive and application thereof in cosmetics |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908069A (en) * | 2019-04-24 | 2019-06-21 | 福州东艺善秀商业有限公司 | A kind of milky lotion of multifunctional plant extractive and preparation method thereof |
| US11786451B2 (en) | 2019-06-28 | 2023-10-17 | The Procter & Gamble Company | Skin care composition |
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