CN106580788A - Mint fermented original pulp cosmetic and preparation method and application thereof - Google Patents
Mint fermented original pulp cosmetic and preparation method and application thereof Download PDFInfo
- Publication number
- CN106580788A CN106580788A CN201611196909.4A CN201611196909A CN106580788A CN 106580788 A CN106580788 A CN 106580788A CN 201611196909 A CN201611196909 A CN 201611196909A CN 106580788 A CN106580788 A CN 106580788A
- Authority
- CN
- China
- Prior art keywords
- preparation
- herba menthae
- mint
- fermentation
- oleo stock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a mint fermented original pulp cosmetic and a preparation method and application thereof. The preparation method of the mint fermented original pulp comprises the following steps: mixing dry mint powder, water and a bacterial fluid to obtain an initial system; fermenting and culturing the initial system to obtain the mint fermented original pulp. In the bacterial fluid, the strain is yeast. The invention also discloses the application of the mint fermented original pulp in the preparation of the cosmetic with at least one function of the following 1)-4): 1) DPPH free radical scavenging; 2 superoxide anion scavenging; 3) antioxidation; and 4) anti aging or the application of the mint fermented original pulp in use direct as the cosmetic with at least one function of the following 1)-4): 1) DPPH free radical scavenging; 2 superoxide anion scavenging; 3) antioxidation; and 4) anti aging. The cosmetic is a mask, essence or toner. The method utilizes the fermentation engineering technology for extracting active substances from mint, and is simple in operation, and due to no addition of any foreign material, the prepared mint fermented product, namely the mint fermented original pulp is harmless to the skin, and has higher anti-aging activity.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of Herba Menthae proferment pulp cosmetic and preparation method thereof with should
With.
Background technology
Herba Menthae for Chun Xing sections plant Herba Menthae dry aerial parts, acrid in the mouth cool in nature, return lung, Liver Channel.China's cultivation history is swung
Long, among the people very early to eat fresh Herba Menthae as vegetable, rear someone makes tea after mentha leave is dried, and begins as medicinal from the Tang Dynasty.
Herba Menthae all herbal medicine, with diaphoresis, dissipate wind-heat and it is antipruritic the effects such as, the composition such as its contained Mentholum and Oleum menthae is pharmaceutically wide
In the general medicine for wind dispelling, anti-corrosion, antiinflammatory, analgesia, antipruritic, stomach invigorating etc..China is always that the outlet of Mentholum and Oleum menthae is big
State, the research to Herba Menthae also deepens continuously.
The safety of active components of plants Yin Qigao and functional activity, have significantly penetrated into food, medicine, cosmetics etc.
Industry.Therefore, extracted from plant using various extractive techniques and have become current study hotspot with highly active composition.
Traditional extractive technique includes organic solvent extraction, hot water extraction method, system solvent extraction method etc., but these method product peaces
It is complete that property is low, time-consuming, extraction ratio is low.The high and environmentally friendly Novel extraction method of safe, extraction ratio increasingly enjoys people to close
Note.It is the various enzymes secreted in growth course using microorganism that microbial method extracts active components of plants, so as to in plant
Active component extracted and modified.
The content of the invention
The preparation method of oleo stock it is an object of the invention to provide a kind of Herba Menthae is fermented.
The preparation method of Herba Menthae fermentation oleo stock provided by the present invention, comprises the steps：By Herba Menthae dry powder, water and bacterium solution
Initial system is mixed to get, fermentation culture is carried out to initial system, obtain Herba Menthae fermentation oleo stock.
In above-mentioned preparation method, the Herba Menthae dry powder is the dry powder of mentha leave, and the dry size-reduced machine of mentha leave is crushed
After sieve and obtain, its particle diameter can be 30～40 mesh, concretely 35 mesh.
In the bacterium solution, strain is yeast, concretely saccharomyces pastorianus bacterium Saccharomyces pastorianus,
ATCC numberings are 28827.
The bacterial concentration can be (105～108)CFU/mL。
The pH value of the bacterium solution can be 6.5～7.5, concretely 7.0
In the initial system, the proportioning of the bacterium solution, Herba Menthae dry powder and water can be (10～15mL)：(13～18) g：
300mL, concretely 12mL：15g：300mL.
The temperature of the fermentation culture can be 40～47 DEG C, concretely 45 DEG C；Time can be 30～45h, concretely
36h。
Above-mentioned preparation method also includes that the fermentation liquid to obtaining after the fermentation culture is sterilized successively, is centrifuged, being taken
The step of clear liquid obtains Herba Menthae fermentation oleo stock.
The condition of the sterilizing is as follows：Sterilising temp can be 115～121 DEG C, concretely 115 DEG C；Sterilization time can be
25～35min, concretely 30min；
The condition of the centrifugation is as follows：Centrifugal rotational speed can be 3800～4200r/min, concretely 4000r/min；Centrifugation
Time can be 10～15min, concretely 10min；Centrifugation radius can be 9cm.
The Herba Menthae fermentation oleo stock that said method is prepared falls within protection scope of the present invention.
Above-mentioned Herba Menthae fermentation oleo stock is being prepared with following 1) -4) at least one of application in the cosmetics of function or
Directly as with following 1) -4) at least one of application in the cosmetics of function, also within the scope of the present invention：
1) DPPH free radicals are removed；
2) superoxide anion is removed；
3) antioxidation；
4) defying age.
In above-mentioned application, the cosmetics are included but is not limited to：Facial film, essence or cosmetic water.
The inventive method extracts active substance using fermentation engineering from Herba Menthae, simple to operate, and due to without
Any foreign substance, the Herba Menthae fermented product for preparing i.e. Herba Menthae fermentation oleo stock resists to skin safe fanout free region with higher
Aging activity.
The present invention has the advantages that：
(1) preparation method of the Herba Menthae fermented product (i.e. Herba Menthae fermentation oleo stock) that the present invention is provided adopts fermentation engineering,
By selecting suitable strain and fermentation condition, extract from Herba Menthae and obtain active substance.On the one hand, plant can at utmost be retained
Active component in thing, and relative to traditional water extraction, it is to avoid the introducing of organic solvent, safety and environmental protection；On the other hand, no
Need to add the foreign substance such as enzyme, not only save production cost, and it is maximized simplify production stage, make the fermentation technique
A large amount of productions, industrialized production can be realized, and the stability of product quality can be fully ensured that.
(2) it is natural that the Herba Menthae fermentation oleo stock that the present invention is provided contains the indispensable aminoacid of healthy skin, mineral etc.
Composition, and due to being not introduced into foreign substance in preparation process, without any chemical composition, can be directly as facial film or elite
The finished product of liquid or cosmetic water is used, more natural than existing other products on the market, will not cause any side effect to skin.
(3) the Herba Menthae fermentation oleo stock that the present invention is provided, can remove DPPH free radicals, promote with stronger oxidation resistance
Collagen protein is generated, and improves body vitality, so as to delay cell senescence, has functions that defying age.
Description of the drawings
Fig. 1 is relation curve of the Herba Menthae fermentation oleo stock to DPPH free radical scavenging activities.
Fig. 2 is relation curve of the Herba Menthae fermentation oleo stock to superoxide anion clearance rate.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, commercially obtain.
Fluid medium in following embodiments is made up of solute and solvent, and solvent is water, solute and its in liquid culture
Concentration in base is：Glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value is 7.0.
YPD culture medium in following embodiments is made up of solute and solvent, and solvent is water, solute and its liquid medium within
In concentration be：Yeast extract 1% (mass fraction), tryptone 2% (mass fraction), glucose 2% (mass fraction),
PH value is 7.0.
Herba Menthae dry powder in following embodiments is 35 mesh.
Saccharomyces pastorianus bacterium Saccharomyces pastorianus in following embodiments, ATCC numbering is 28827.
Zymocyte bacterium solution in following embodiments is obtained in accordance with the following steps：
1st, the activation of strain
The ring of picking saccharomyces pastorianus bacterium bacterium colony one is put into by actication of culture in shaking table, after being activated in fluid medium
Saccharomyces pastorianus bacterium.
2nd, the purification of strain
Bacterium solution gradient dilution bed board after the activation that step 1 is obtained, to obtain single bacterium colony, obtains newborn bar after purification
Bacterium.
3rd, the amplification culture of strain
The strain after purification that step 2 is obtained is inoculated in YPD culture medium (pH value is 7.0), in 25 DEG C of shaking table
Culture, as OD values=0.5-1.0, (strain is in logarithmic (log) phase, and concentration is 10 to obtain zymocyte bacterium solution5～108CFU/mL)。
Embodiment 1, preparation Herba Menthae fermentation oleo stock
Herba Menthae fermentation oleo stock is prepared in accordance with the following steps：
(1) concentration for obtaining 12mL steps 3 is 105～108The zymocyte bacterium solution of CFU/mL is inoculated into the Herba Menthae of 15g and does
In the water of powder and 300mL, fermentation system is obtained；
(2) above-mentioned fermentation system is fermented 36 hours in 45 DEG C of shaking tables, obtains tunning；
(3) by the 30min that sterilizes at 115 DEG C of above-mentioned tunning, inactivate bacterium, the tunning after being sterilized；To go out
Tunning after bacterium is that 10min is centrifuged under conditions of 9cm in 4000r/min, centrifugation radius, abandons precipitation, collects supernatant, i.e.,
For Herba Menthae fermentation oleo stock.
Application of embodiment 2, the Herba Menthae fermentation oleo stock in as cosmetics
First, the property of Herba Menthae proferment pulp cosmetic
By the fermentation oleo stock Jing activated carbon decolorizing of the prepared gained of embodiment 1, de-taste.Its outward appearance is thick liquid, color
For faint yellow to brown color.PH value 6.2-7.0, viscosity 190-230cP, solubility Solid content content 3.0-3.5%, total plate count
Less than 50CFU/mL, without pathogenic bacterium detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than
1000CFU/ml, so this fermentation oleo stock meets cosmetics quality requirement.
Component analyses are carried out to the Herba Menthae fermentation oleo stock, crude polysaccharides detection method is with reference to GB/T 5009.8-2008；Flavone
, with reference to GB/T 5009.124-2003, protein detection method is with reference to GB5009.5-2010, total phenols detection method ginseng for detection method
According to GB/T 8313-2008, acquired results are as follows：
Obtained Herba Menthae fermentation oleo stock 9.08g/kg containing crude polysaccharides of the invention, total flavones 0.15g/kg, total protein 4.92g/
Kg, total phenols 1.39g/kg.
2nd, the safety detection of Herba Menthae fermentation oleo stock
Human body patch test is mainly used for detecting the zest of cosmetics finished product or raw material.The present invention is to embodiment 1
The Herba Menthae fermentation oleo stock of middle acquisition carries out human closed formula patch test, it is intended to which its potential skin irritation is estimated.
1st, subjects
The people of suitable volunteer 30 is selected, the range of age is in 18-60 year random selection.
2nd, test method
Weigh 0.020g-0.025g solid samples or semisolid sample is put into speckle and tries standby in device.By fluid sample
0.2mL to 0.025mL Deca is on filter paper, then filter paper is placed in speckle examination device.Each sample is respectively provided with blank,
The sample solvent with sample equivalent, such as distilled water or olive oil are added in control speckle examination device hole (the present embodiment adopts distilled water).
Human body back is elected at test position as, is fixed speckle examination device using nonirritant adhesive tape and is pasted on experimenter back.
Test period continues 24h.For accurate, the credible and science of result of the test, volunteer is as requested during testing, it is impossible to pluck
Fall speckle examination device, can not also make recipient site contact water.Speckle examination device is removed after 24h, after standing 30min, waits impression to disappear, observation
The reaction of skin.If result of the test is feminine gender, 24h and 48h after patch test is needed to observe again once respectively.
3rd, result of the test
As shown in table 1, the implication that each symbol is represented in table 1 is as follows for patch test results："-"=negative reaction；" ± "=
Suspicious reaction：Only faint erythema；"+"=weakly positive reaction (erythematous response)：Erythema, infiltration, edema, there can be pimple；"++"
=strong positive reaction (herpess reaction)；Erythema, infiltration, edema, pimple, herpess；Reaction can exceed tested area；" +++ "=it is extremely strong
Positive reaction (reaction of amalgamation herpess)；Obvious erythema, severe infiltration, edema, amalgamation herpess；Reaction exceeds tested area.
Criterion：According to the number for occurring 1 grade of skin adverse reaction in 2007,30 experimenters of cosmetics health specification
More than 5, or the number for two grades of skin adverse reactions occur is more than 2, or any 1 three-level of appearance or three-level above skin are not
During good reaction, then judge that tested material has untoward reaction to human body, conversely, be then considered as being had no adverse reaction to human body.
The patch test results of the Herba Menthae fermentation oleo stock that table 1, embodiment 1 are obtained
As can be seen from Table 1：The Herba Menthae fermentation oleo stock that embodiment 1 is obtained produces 1 suspicious reaction, illustrates that the present invention is carried
For Herba Menthae fermentation oleo stock there is safety, body will not be given people and bring untoward reaction.
3rd, the antioxygenic property detection of Herba Menthae fermentation oleo stock
DPPH is a kind of organic free radical of early stage synthesis, is commonly used to assess the hydrogen supply capacity of polyphenoils, and it is organic
It is highly stable in solvent, in purple, and there are a characteristic absorption peak, when free radical scavenger is run into, DPPH at 517nm
Lone pair electrons be paired and make it fade, that is, diminish in the light absorption value of maximum absorption wave strong point.Therefore, can be by determining
The change of light absorption value is evaluating elimination effect of the sample to DPPH free radicals.
Take the Herba Menthae fermentation oleo stock that different amounts of embodiment 1 prepares to be dissolved in deionized water, volume basis are obtained dense
Spend a series of Herba Menthaes fermentation oleo stock prepare liquid for 0.63%, 1.25%, 2.5%, 5% and 10%.
DPPH free radical scavengings experiment specific experiment step be：
(1) prepare liquid and 2 × 10 of equal-volume (generally 3mL) is taken-4The DPPH solution of mol/L mixes (A1Pipe)；
(2) isopyknic dehydrated alcohol (determinand solvent) and 2 × 10 is taken-4The DPPH solution of mol/L mixes (A2Pipe)；
(3) take isopyknic dehydrated alcohol and mix (A with prepare liquid3Pipe)；
(4) react after 30min, under 517nm A is surveyed1、A2、A3Pipe absorbance.
Clearance rate computing formula is：Clearance rate (%)=[(A2+A3)-A1]/A2 (1)
As abscissa, clearance rate is vertical coordinate to concentration expressed in percentage by volume with Herba Menthae fermentation oleo stock prepare liquid, makes Herba Menthae and sends out
Ferment oleo stock is shown in Fig. 1 to DPPH free radical scavenging effect curves.
As shown in Figure 1, the gained Herba Menthae of embodiment 1 fermentation oleo stock reaches after diluting 10 times to DPPH free radical scavenging activities
89.41%, the Herba Menthae fermentation oleo stock of 1.33% (concentration expressed in percentage by volume) can remove 50% DPPH free radicals, illustrate that Herba Menthae is fermented
There is oleo stock very strong oxidation resistance can remove free radical, promote cellular metabolism, strengthen cell viability, improve the structure of body
And function, body vitality is improved, so as to delay cell senescence, play the effect of its defying age.
4th, the specific experiment step that superoxide anion removes experiment is
(1) the Tris-HCl buffer of 4.5ml 0.05mol/L pH8.2 is taken in test tube, add 4ml deionized waters,
0.1ml samples, mix, and are placed in 25 DEG C of water-baths and preheat 10min；
(2) pyrogallol solution (2.5mmol/L, the 10mmol/L of 25 DEG C of insulation 10min of 0.4mL are rapidly added after taking out
HCl is prepared).
(3) 8mol/L hydrochloric acid 1ml terminating reactions, blank group is added to compare with distilled water.
(4) mensuration absorbance at 299nm, calculates clearance rate.A1 is sample, and A2 is compareed.
Superoxide anion clearance rate (%)=(A1-A2)/A1.
As shown in Fig. 2 Herba Menthae fermentation oleo stock has significant scavenging action to superoxide anion.It is dense with Herba Menthae fermentation oleo stock
Degree is raised, and its clearance rate to superoxide anion gradually increases.Higher than after 50% (concentration expressed in percentage by volume), it is removed oleo stock concentration
The ability of superoxide anion increases slow.100% Herba Menthae ferment oleo stock to the Scavenging activity of superoxide anion 86% or so,
Removing 50% superoxide anion needs the Herba Menthae fermentation oleo stock of 26.12% (concentration expressed in percentage by volume).
Claims (10)
1. a kind of preparation method of Herba Menthae fermentation oleo stock, comprises the steps：Herba Menthae dry powder, water and bacterium solution are mixed to get initially
System, to initial system fermentation culture is carried out, and obtains Herba Menthae fermentation oleo stock.
2. preparation method according to claim 1, it is characterised in that：In the preparation method, the bacterial concentration is (105
～108)CFU/mL；
In the initial system, the proportioning of the bacterium solution, Herba Menthae dry powder and water is (10～15mL)：(13～18) g：(300)mL.
3. preparation method according to claim 1 and 2, it is characterised in that：The temperature of the fermentation culture is 40～47 DEG C；
Time is 30～45h.
4. the preparation method according to any one of claim 1-3, it is characterised in that：In the bacterium solution, strain is yeast
Bacterium；
The Herba Menthae dry powder is the dry powder of mentha leave, and its particle diameter is 30～40 mesh.
5. according to the preparation method described in claim 4, it is characterised in that：The yeast is saccharomyces pastorianus bacterium
Saccharomyces pastorianus。
6. the preparation method according to any one of claim 1-5, it is characterised in that：The preparation method is also included to institute
State the fermentation liquid obtained after fermentation culture sterilized successively, be centrifuged, take supernatant obtain Herba Menthae fermentation oleo stock the step of.
7. preparation method according to claim 6, it is characterised in that：The condition of the sterilizing is as follows：Sterilising temp is 115
～121 DEG C；Sterilization time is 25～35min；
The condition of the centrifugation is as follows：Centrifugal rotational speed is 3800～4200r/min；Centrifugation time is 10～15min；Centrifugation radius
For 9cm.
8. the Herba Menthae fermentation oleo stock that the preparation method by any one of claim 1-7 is prepared.
9. described in claim 8 Herba Menthae fermentation oleo stock prepare with following at least one functions cosmetics in application or
Application in directly as the cosmetics with following at least one functions：
1) DPPH free radicals are removed；
2) superoxide anion is removed；
3) antioxidation；
4) defying age.
10. application according to claim 9, it is characterised in that：The cosmetics are facial film, essence or cosmetic water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611196909.4A CN106580788A (en) | 2016-12-22 | 2016-12-22 | Mint fermented original pulp cosmetic and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611196909.4A CN106580788A (en) | 2016-12-22 | 2016-12-22 | Mint fermented original pulp cosmetic and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106580788A true CN106580788A (en) | 2017-04-26 |
Family
ID=58602402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611196909.4A Pending CN106580788A (en) | 2016-12-22 | 2016-12-22 | Mint fermented original pulp cosmetic and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106580788A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147586A (en) * | 2015-09-30 | 2015-12-16 | 上海全丽生物科技有限公司 | Rose fermentation puree as well as preparation method and application thereof |
| CN105902812A (en) * | 2016-05-15 | 2016-08-31 | 王紫文熙 | Traditional Chinese medicine composition with beauty treatment effect, facial mask cream, and preparation methods of traditional Chinese medicine composition and facial mask cream |
-
2016
- 2016-12-22 CN CN201611196909.4A patent/CN106580788A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147586A (en) * | 2015-09-30 | 2015-12-16 | 上海全丽生物科技有限公司 | Rose fermentation puree as well as preparation method and application thereof |
| CN105902812A (en) * | 2016-05-15 | 2016-08-31 | 王紫文熙 | Traditional Chinese medicine composition with beauty treatment effect, facial mask cream, and preparation methods of traditional Chinese medicine composition and facial mask cream |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105147586A (en) | Rose fermentation puree as well as preparation method and application thereof | |
| CN105534876A (en) | Dendrobe fermentation raw stock as well as preparation method and application thereof | |
| CN106511180A (en) | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof | |
| CN105193658A (en) | Cosmetic with fermented original rice milk, method for manufacturing cosmetic and application thereof | |
| CN105395468A (en) | Cactus fermented raw stock and preparation method and application thereof | |
| CN105147587A (en) | Ganoderma lucidum fermentation protoplasm cosmetic and preparation method and application thereof | |
| CN105147588A (en) | Ginseng fermentation puree as well as preparation method and application thereof | |
| CN104257598A (en) | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof | |
| CN105919889B (en) | A kind of blueness bur proferment pulp cosmetic and the preparation method and application thereof | |
| CN105002254A (en) | Preparation method and application of tremella fermentation extract | |
| CN110680774A (en) | Preparation method of jasmine double-bacterium fermented cosmetic and application of product | |
| CN106580789A (en) | Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof | |
| CN106726949B (en) | Grape seed fermentation raw stock cosmetic and preparation method and application thereof | |
| CN111419752B (en) | Four-substance fermented water and preparation method and application thereof | |
| CN110680781A (en) | White peony root fermented cosmetic and preparation method and application thereof | |
| CN105147564A (en) | Purslane fermentation protoplasm ceramic and preparation method and application | |
| CN105250168A (en) | High mountain tea fermentation raw stock cosmetic and preparation method and application thereof | |
| CN112245343B (en) | Method for fermenting burdock root by lucid ganoderma, method for compositely fermenting burdock root, fermented product and application | |
| CN109939051A (en) | A kind of mulberries fermentation magma and its preparation method and application | |
| CN109363984A (en) | A kind of prinsepia utilis royle oil dregs of rice fermentation liquid and preparation method thereof and the application in cosmetics | |
| CN105250169A (en) | Fermented aloe raw stock cosmetic and preparation method and application thereof | |
| CN109288750B (en) | Lycium ruthenicum fermentation liquor, preparation method thereof and application thereof in cosmetics | |
| CN105925617A (en) | Radix pueraiae fermentation protoplasm cosmetic product and preparation method and application thereof | |
| CN115006297A (en) | Revival grass fermentation extracting solution, preparation method and application | |
| CN115025033A (en) | Mulberry leaf fermented composition, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170426 |