CN109125164A - A kind of lycium ruthenicum fermentation magma and preparation method thereof - Google Patents

A kind of lycium ruthenicum fermentation magma and preparation method thereof Download PDF

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Publication number
CN109125164A
CN109125164A CN201810959377.8A CN201810959377A CN109125164A CN 109125164 A CN109125164 A CN 109125164A CN 201810959377 A CN201810959377 A CN 201810959377A CN 109125164 A CN109125164 A CN 109125164A
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lycium ruthenicum
fermentation
magma
fermented
bacterium solution
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方国忠
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SHANGHAI DAI COSMETICS INSTRUMENT Ltd
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SHANGHAI DAI COSMETICS INSTRUMENT Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74Biological properties of particular ingredients
    • A61K2800/78Enzyme modulators, e.g. Enzyme agonists
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Engineering & Computer Science (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to field of biotechnology, specifically a kind of lycium ruthenicum fermentation magma and preparation method thereof, which is characterized in that fermentation liquid obtained by the initial system fermentation for being obtained by mixing lycium ruthenicum dry powder, water and bacterium solution is sterilized, supernatant obtained by centrifugation;The present invention is compared to the prior art, the vegetable active in product is remained to greatest extent, and any organic reagent is not added when preparing, fermentation temperature and fermentation pH are mild, active components of plants structure is not destroyed, the natural activity of plant is kept, and does not add the chemical components such as essence in magma of fermenting, it is ensured that safety of the product to human body.

Description

A kind of lycium ruthenicum fermentation magma and preparation method thereof
Technical field
The present invention relates to field of biotechnology, specifically a kind of lycium ruthenicum fermentation magma and preparation method thereof.
Background technique
Lycium ruthenicum is Solanaceae Lycium.Mainly it is grown on the ground such as northwest China Qinghai.Lycium ruthenicum is rich in lot of trace Element, the ingredients such as carrotene, polysaccharides, amino acid are precious medical and edible dual purpose plants.Cyanine contained in lycium ruthenicum Element has reduction blood lipid, anti-inflammatory, anti-radiation and anti-aging and other effects.The application of lycium ruthenicum is mainly in the preparation side of health care product Lycium ruthenicum soak is directly such as drunk, or is prepared into lycium ruthenicum effervescent tablet and drinks in face.Current fermentation technique is widely used to people Life in, if Yoghourt preparation, wine brewing, soy sauce, now by fermentation fruits and vegetables obtain ferment, can be used as health care product use.Hair During ferment, microorganism can decompose the secondary metabolites such as cellulase, amylase, lignoenzyme, act on plant cell, increase Added with effect Ingredients Active, toxic side effect is reduced.The present invention carries out bioconversion to lycium ruthenicum using the method for fermentation, obtains black Chinese holly Qi fermentation magma, has good anti-aging effects.
Currently, safety and the functional activity of active components of plants Yin Qigao, significantly penetrate into food, medicine, change The industries such as cosmetic.Therefore, the ingredient with high activity is extracted from plants using various extractive techniques and has become current grind Study carefully hot spot.Traditional extractive technique includes organic solvent extraction, hot water extraction method, system solvent extraction method etc., but these sides Method Product Safety is low, time-consuming, recovery rate is low.The high and environmentally friendly Novel extraction method of highly-safe, recovery rate is more and more standby It is paid close attention to by people.
Therefore, there are also to be developed for the prior art.
Summary of the invention
In view of above-mentioned deficiencies of the prior art place, the purpose of the present invention is to provide a kind of lycium ruthenicum fermentation magma and its Preparation method.The problem of aiming to solve the problem that existing extractive technique time-consuming, inefficiency.
In order to achieve the above object, this invention takes following technical schemes:
The present invention provides a kind of lycium ruthenicum fermentation magma, which is characterized in that
Fermentation liquid obtained by the initial system fermentation that lycium ruthenicum dry powder, water and bacterium solution are obtained by mixing is sterilized, centrifugation and The supernatant obtained;
The fruit of the lycium ruthenicum dry powder from lycium ruthenicum.
Further, the bacterium solution: lycium ruthenicum dry powder: water=5~10mL: 18~22g: 280mL.
Further, the strain in the bacterium solution is rhodotorula mucilaginosa.
Further, the concentration of the bacterium solution is 105~108CFU/mL。
Further, the pH value of the bacterium solution can be 6.5~7.5.
The present invention also provides a kind of methods for preparing the lycium ruthenicum fermentation magma, which is characterized in that including following step It is rapid:
1) the lycium ruthenicum dry powder, water and bacterium solution are mixed, obtains initial system;
2) fermented and cultured is carried out to initial system;
3) fermentation liquid obtained to fermented culture is successively sterilized, is centrifuged, takes supernatant, and lycium ruthenicum proferment is made Slurry.
Further, the temperature of fermented and cultured described in step 2) is 35~45 DEG C, concretely 37 DEG C;Time be 45~ 55h, concretely 48h.
Further, the temperature of sterilizing described in step 3) is 115~121 DEG C, concretely 115 DEG C;Sterilization time is 25~35min, concretely 30min;The revolving speed of the centrifugation is 3800~4200r/min, concretely 4000r/min;From The heart time is 10~15min, concretely 10min;Centrifugation radius is 9cm.
The present invention also provides lycium ruthenicum fermentation magma in the cosmetics that preparation has following at least one functions Application or directly as the application in the cosmetics with following at least one functions:
1) DPPH free radical is removed;
2) ABTS free radical is removed;
3) promote fibroblast proliferation;
4) promote collagen synthesis;
5) increase catalase activity;
6) increase glutathione photooxide phototroph enzymatic activity;
7) senile-resistant efficacy.
Further, the cosmetics are facial mask, Essence or toner.
Compared with prior art, the present invention having the advantages that
(1) preparation method of lycium ruthenicum fermentation magma provided by the invention uses fermentation engineering, suitable by selecting Strain and fermentation condition, from lycium ruthenicum extract obtain active material.On the one hand, the activity in plant can utmostly be retained Ingredient, and relative to traditional water extraction, avoid the introducing of organic solvent, safety and environmental protection;On the other hand, addition enzyme is not needed Equal foreign substances, not only save production cost, but also maximumlly simplify production stage, can be realized the fermentation technique greatly Amount production, industrialized production, and can fully ensure that the stability of product quality.
(2) lycium ruthenicum fermentation magma provided by the invention contains the indispensable amino acid of healthy skin, minerals etc. certainly Right ingredient, and due to being not introduced into foreign substance during the preparation process, it, can be directly as facial mask or essence without any chemical component The finished product of magnificent liquid or toner uses, more natural than existing other products on the market, and any negative work will not be caused to skin With.
(3) lycium ruthenicum provided by the invention is fermented magma, have stronger oxidation resistance can remove DPPH free radical and Hydroxy radical promotes fibroblast proliferation, promotes collagen synthesis, improves catalase and glutathione peroxidase Activity, to delay cell senescence, has anti-aging function to improve body vitality.
Detailed description of the invention
Fig. 1 is the result schematic diagram that lycium ruthenicum fermentation magma removes DPPH free radical and ABTS free radical;
Fig. 2 is influence result schematic diagram of the lycium ruthenicum fermentation magma to fibroblast survival rate;
Fig. 3 is the relative expression quantity schematic diagram that lycium ruthenicum fermentation magma acts on lower collagen 1 and MMP-1;
Fig. 4 is influence result schematic diagram of the lycium ruthenicum fermentation magma to activity of catalase;
Fig. 5 is influence result schematic diagram of the lycium ruthenicum fermentation magma to glutathione peroxidase activity;
Fig. 6 is intracellular relative intensity of fluorescence schematic diagram under the effect of lycium ruthenicum fermentation liquid.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Fluid nutrient medium in following embodiments is made of solute and solvent, and solvent is water, solute and its in Liquid Culture Concentration in base are as follows: glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value 7.0.
YPD culture medium in following embodiments is made of solute and solvent, and solvent is water, solute and its liquid medium within In concentration are as follows: yeast extract 1% (mass fraction), tryptone 2% (mass fraction), glucose 2% (mass fraction), PH value is 7.0.
Lycium ruthenicum dry powder (fruit from lycium ruthenicum) in following embodiments is limited from Qinghai Kang Pu biotechnology share Company, 30 mesh.
Rhodotorula mucilaginosa (Rhodotorula mucilaginosa), lactobacillus, saccharomyces cerevisiae in following embodiments are purchased from China General Microbiological Culture Collection Center.
Lycium ruthenicum bacterium solution in following embodiments is made in accordance with the following steps:
1, the activation of strain
Rhodotorula mucilaginosa, lactobacillus, one ring of saccharomyces cerevisiae bacterium colony are picked them separately in fluid nutrient medium, is put into bacterium in shaking table Kind activation, the bacterium solution after being activated.
2, the purifying of strain
Bacterium solution gradient dilution bed board after the activation that step 1 is obtained obtains the red ferment of glue after purification to obtain single colonie Mother, lactobacillus, saccharomyces cerevisiae.
3, the expansion culture of strain
The strain after purification that step 2 is obtained is inoculated into respectively in YPD culture medium (pH value 7.0), in 25 DEG C shake It is cultivated in bed, as OD value=0.5-1.0, obtaining zymophyte bacterium solution, (strain is in logarithmic phase, and concentration is 105~108CFU/ mL)。
Embodiment 1
Lycium ruthenicum fermentation magma is prepared in accordance with the following steps:
(1) concentration for obtaining the above-mentioned steps of 8mL 3 is 105~108The zymophyte bacterium solution of CFU/mL is inoculated into the black of 20g In the water of fructus lycii dry powder and 300mL, fermentation system is obtained;
(2) above-mentioned fermentation system is fermented 48 hours in 37 DEG C of shaking tables, obtains tunning;
(3) by the 30min that sterilizes at 115 DEG C of above-mentioned tunning, inactivate bacterium, the tunning after being sterilized;It will go out Tunning after bacterium is centrifuged under conditions of radius is 9cm in 4000r/min and is centrifuged 10min, abandon precipitating, collect supernatant, i.e., For lycium ruthenicum fermentation magma.
Embodiment 2
Application of the lycium ruthenicum fermentation magma in cosmetics is as follows:
One, the property of lycium ruthenicum fermentation magma
By resulting fermentation magma prepared by embodiment 1 through active carbon decoloring, de-taste.Its appearance is thick liquid, color To be faint yellow to brown color.PH value 6.1-6.8, viscosity 190-230cP, soluble solids content 3.0-3.5%, total plate count Less than 50CFU/mL, no pathogenic bacteria detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than 1000CFU/ml, so this fermentation magma meets cosmetics quality requirement.
Constituent analysis is carried out to lycium ruthenicum fermentation magma, Thick many candies detection method is referring to GB/T 5009.8-2008;It is yellow Ketone detection method is referring to GB/T 5009.124-2003, and protein detection method is referring to GB5009.5-2010;Total phenol detection method It is as follows referring to GB/T 8313-2008 acquired results:
Constituent analysis (mg/mL) of 1 different strains of table to lycium ruthenicum fermentation magma
Containing Thick many candies General flavone Total phenol Total protein
Rhodotorula mucilaginosa 36.2 0.034 0.128 2.00
Lactobacillus 28.4 0.026 0.084 1.83
Saccharomyces cerevisiae 26.3 0.016 0.064 1.73
As it can be seen that the present invention using the fermentation magma of lycium ruthenicum made from rhodotorula mucilaginosa containing Thick many candies, general flavone 0.034, total phenol, Total protein is most.
Two, the safety detection of lycium ruthenicum fermentation magma
Human body patch test is mainly used for detecting the irritation of cosmetic end product or raw material.The present invention is to embodiment 1 The fermentation magma of lycium ruthenicum made from the rhodotorula mucilaginosa of middle acquisition carries out human closed formula patch test, it is intended to pierce to its potential skin Sharp property is assessed.
1, subjects
Suitable 30 people of volunteer is selected, the range of age was randomly choosed at 18-60 years old.
2, test method
Weigh 0.020g-0.025g solid sample or semisolid sample be put into it is spare in spot tester.By fluid sample 0.2mL to 0.025mL dropwise addition is placed in spot tester on filter paper, then by filter paper.Each sample is respectively provided with blank control, The sample solvent being added in spot tester hole with sample equivalent is compareed, such as distilled water or olive oil (the present embodiment uses distilled water).
Experiment position is selected as human body back, and spot tester fixation is pasted on subject back using nonirritant adhesive tape. Test period continues for 24 hours.It is accurate, credible and scientific for test result, dduring test volunteer as requested, Bu Nengzhai Fall spot tester, recipient site can not also be made to contact water.It removes spot tester afterwards for 24 hours, after standing 30min, impression is waited to disappear, observation The reaction of skin.If test result is feminine gender, need to observe again respectively with 48h for 24 hours after patch test primary.
3, test result
Patch test results are as shown in table 2, and the meaning that each symbol indicates in table 2 is as follows: "-"=negative reaction;" ± "= Suspicious reaction: only faint erythema;"+"=weakly positive reaction (erythematous response): erythema, oedema, can have papule at infiltration;″++″ =strong positive reaction (bleb reaction);Erythema, infiltration, oedema, papule, bleb;Reaction can exceed tested area;" +++ "=it is extremely strong Positive reaction (reaction of amalgamation bleb);Obvious erythema, severe infiltration, oedema, amalgamation bleb;Reaction exceeds tested area.
Judgment criteria: according to the number of 1 grade of skin adverse reaction of appearance in 2007,30 subjects of cosmetics health specification More than 5, or there is the number of second level skin adverse reaction more than 2, or any 1 three-level or the above skin of three-level occur not When good reaction, then determine that tested material has adverse reaction to human body, has no adverse reaction to human body conversely, being then considered as.
The patch test results for the lycium ruthenicum fermentation magma that table 2, embodiment 1 obtain
As can be seen from Table 2: the lycium ruthenicum fermentation magma that embodiment 1 obtains generates 1 suspicious reaction, illustrates the present invention The lycium ruthenicum fermentation magma of offer all has safety, will not bring adverse reaction to human body.
Three, the antioxygenic property detection DPPH radicals scavenging experiment of lycium ruthenicum fermentation magma
A1Pipe: the sample of 3mL and the DPPH solution (2 × 10 of 3mL are taken-4Mol/L it) mixes;A2Pipe: the water and consubstantiality of 3mL are taken Long-pending DPPH solution mixes;A3Pipe: the dehydrated alcohol and isometric sample blending of 3mL are taken;Reaction 30min surveys A under 517nm1, A2And A3The absorbance of pipe.DPPH free radical scavenging activity=[(A2+A3)-A1]/A2× 100%.
The fermentation magma of lycium ruthenicum made from rhodotorula mucilaginosa acts on obviously DPPH radicals scavenging, with lycium ruthenicum fermentation magma Concentration increases, and the ability for removing DPPH free radical gradually increases, and shows that lycium ruthenicum fermentation magma removes the energy of DPPH free radical Power and its concentration just have significant dose-effect relationship.25% lycium ruthenicum fermentation magma can remove nearly 50% DPPH free radical.
ABTS radicals scavenging experiment
Prepared ABTS working stocks are taken, room temperature is protected from light storage 12-16h, is diluted, with 80% ethyl alcohol using preceding After asking the absorbance of ABTS working solution to subtract the control of 80% ethanol blank, A734 is 0.7 ± 0.05.It is accurate to measure each dense of 10 μ L The ABTS working solution of the sample solution of degree and 200 μ L mix gently in 96 orifice plates, measure at 734nm after reacting 2-6min Its absorbance value.The clearance rate of ABTS is calculated as follows:
ABTS clearance rate (%)=1- (At-B)/A0× 100%
In formula: A0It is not loaded;The absorbance value of ABTS;AtAbsorbance value after being reacted for sample with ABTS;B is sample The absorbance value of product blank.
Lycium ruthenicum made from rhodotorula mucilaginosa ferments magma to extremely obvious, 3.12% lycium ruthenicum of ABTS radicals scavenging effect Fermentation magma can remove 89% ABTS free radical, referring to Fig. 1.
Four, lycium ruthenicum fermentation magma facilitates fibrocyte proliferation to test
Logarithmic phase cell is collected, concentration of cell suspension is adjusted, 100 μ l (96 orifice plate) is added in every hole, and bed board makes cell to be measured Adjust density to 5000/hole, (edge hole is filled with sterile PBS).5%CO2,37 DEG C of incubations, until cell grows to certain density, The candidate drug that various concentration gradient is added in liquid is changed, is control with the culture solution without candidate drug.5%CO2,37 DEG C are incubated for 24 Hour, 20 μ lMTT solution (5mg/ml, i.e. 0.5%MTT) are added in every hole, continue to cultivate 4h.React drug sufficiently with MTT, it can It is first centrifuged and discards culture solution afterwards, carefully rush 2-3 after with PBS, add the culture solution containing MTT.Culture is terminated, hole is carefully sucked Interior culture solution.150 μ l dimethyl sulfoxides are added in every hole, set low-speed oscillation 10min on shaking table, dissolve crystal sufficiently.In enzyme Join the light absorption value in each hole of measurement at immune detector OD value 490nm.Cell viability=(measurement hole OD value-blank control OD value)/ (cell controls group OD value-blank control OD value) * 100%.
Fibroblast is the host cell ingredient in dermis of skin, it can not only synthesize and secrete collagenous fibres, bullet Property fiber, Matrix protein substance and certain growth factors, while fibroblast also has adhesion characteristics and chemotaxis, to dimension The elasticity and toughness for holding skin play a significant role.With advancing age, skin gradually aging shows as cutis laxa, wrinkle Line increases, it is coarse to dry, elastic reduces etc..These performances are all reduced with fibroblast quantity, synthesis function reduction has closely Relationship.As shown in Fig. 2, the fermentation magma of lycium ruthenicum made from rhodotorula mucilaginosa in 5% concentration range is to fibroblast without poison Property, survival rate is 100% or more.The fermentation Chinese wolfberry fruit magma effect of 1.66%-5% concentration has fibroblast proliferation certain Facilitation, referring to fig. 2.
Five, lycium ruthenicum fermentation magma promotes collagen I synthesis detection
The sample for taking debita spissitudo after acting on fibroblast for 24 hours, collects cell culture fluid, 2000- using sterile tube 3000rpm is centrifuged 20min, saves supernatant.People's type i collagen (COL I) enzyme linked immunosorbent detection examination of production is built up using Nanjing Agent box is detected.
1% concentration lycium ruthenicum fermentation magma has facilitation to collagen synthesis, and collagen synthesis is pair of blank According to 147.83%, referring to Fig. 3.
Six, lycium ruthenicum fermentation magma inhibitory activity oxygen content detection
Active o content detection uses the active oxygen detection kit (S0033) in the green skies.Take 0.2%, 1%, 5%, 1% The lycium ruthenicum fermentation liquid of EGCG and Rosup is filtered through 0.22 μm of sterilised membrane filter, and being added to culture has fibroblastic 96 orifice plate In, after culture for 24 hours, DCFH probe is loaded, in 37 DEG C of incubation 20min, in 488nm after washing cell three times with serum free medium Excitation wavelength, 525nm transmitted wave strong point observes each instrument connection should intensity.
After fluorescent dyeing, intensity of cellular fluorescence is directly proportional to active o content, referring to Fig. 6.As shown in Fig. 2, Under the lycium ruthenicum fermentation magma effect of 0.2%, 1% and 5% concentration, active o content is significantly below sky in fibroblast Leucocyte.Lycium ruthenicum magma can reduce the generation of interior free yl to the inhibiting effect of active oxygen, thus delaying senility.
Seven, lycium ruthenicum fermentation magma enhancing catalase activity detection
(S0051) is detected using the hydrogen peroxide enzyme detection kit in the green skies.Take 3 μ l samples to 1.5ml centrifugation Guan Zhong, it is 40 μ l that catalase, which is added, and detects buffer to volume, adds 10 μ l 250mM hydrogenperoxide steam generators, mixed rapidly It is even.Catalase reaction terminate liquid is added after reacting a period of time, mixes and terminates reaction.It takes 10 μ l to terminate reaction solution to add Enter 40 μ l catalases detection buffer, mix, is measured at 520nm after adding developing solution.
Under the lycium ruthenicum fermentation magma effect of 0.2%~5% concentration, the activity of catalase in fibroblast is with black Fructus lycii concentration, which enhances vigour, to be gradually increased, referring to fig. 4.
Eight, lycium ruthenicum fermentation magma enhances glutathione photooxide phototroph Enzyme assay
It is detected using green skies S0056 glutathione peroxidase detection kit.
Glutathione peroxidase (GPX) is one of antioxidase important in living organism, it can eliminate body Interior hydrogen peroxide and lipid peroxide, further damage of the blocking activity oxygen radical to body, is important in organism Active oxygen radical scavenger.Lycium ruthenicum fermentation magma has rush to the glutathione photooxide phototroph enzymatic activity in fibroblast Into effect, when under the lycium ruthenicum fermentation magma effect in 0.2%~5% concentration range, activity of glutathione peroxidase by It is cumulative strong, referring to Fig. 5.

Claims (10)

  1. The magma 1. a kind of lycium ruthenicum is fermented, which is characterized in that
    Fermentation liquid obtained by the initial system fermentation that lycium ruthenicum dry powder, water and bacterium solution are obtained by mixing is sterilized, obtained by centrifugation Supernatant;
    The fruit of the lycium ruthenicum dry powder from lycium ruthenicum.
  2. The magma 2. a kind of lycium ruthenicum according to claim 1 is fermented, which is characterized in that the bacterium solution: lycium ruthenicum dry powder: water =5~10mL:18~22g:280mL.
  3. The magma 3. a kind of lycium ruthenicum according to claim 1 is fermented, which is characterized in that the strain in the bacterium solution is that glue is red Yeast.
  4. The magma 4. a kind of lycium ruthenicum according to claim 1 is fermented, which is characterized in that the concentration of the bacterium solution is 105~ 108CFU/mL。
  5. The magma 5. a kind of lycium ruthenicum according to claim 1 is fermented, which is characterized in that the pH value of the bacterium solution is 6.5~ 7.5。
  6. 6. a kind of method for preparing Claims 1 to 5 described in any item lycium ruthenicums fermentation magma, which is characterized in that including with Lower step:
    1) the lycium ruthenicum dry powder, water and bacterium solution are mixed, obtains initial system;
    2) fermented and cultured is carried out to initial system;
    3) fermentation liquid obtained to fermented culture is successively sterilized, is centrifuged, takes supernatant, and lycium ruthenicum fermentation magma is made.
  7. 7. a kind of preparation method of lycium ruthenicum fermentation magma according to claim 6, which is characterized in that described in step 2) The temperature of fermented and cultured is 35~45 DEG C;Time is 45~55h.
  8. 8. a kind of preparation method of lycium ruthenicum fermentation magma according to claim 6, which is characterized in that described in step 3) The temperature of sterilizing is 115~121 DEG C;Sterilization time is 25~35min;The revolving speed of the centrifugation is 3800~4200r/min;From The heart time is 10~15min;Centrifugation radius is 9cm.
  9. 9. described in any item lycium ruthenicum fermentation magma have following at least one functions in preparation according to claim 1~5 In cosmetics application or directly as the application in the cosmetics with following at least one functions:
    1) DPPH free radical is removed;
    2) ABTS free radical is removed;
    3) promote fibroblast proliferation;
    4) promote collagen synthesis;
    5) increase catalase activity;
    6) increase glutathione photooxide phototroph enzymatic activity;
    7) senile-resistant efficacy.
  10. 10. application according to claim 9, it is characterised in that: the cosmetics are facial mask, Essence or toner.
CN201810959377.8A 2018-08-22 2018-08-22 A kind of lycium ruthenicum fermentation magma and preparation method thereof Pending CN109125164A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674506A (en) * 1994-03-04 1997-10-07 Pacific Corporation Make-up cosmetic compositions containing powdered phaffia yeast
CN104257598A (en) * 2014-09-18 2015-01-07 北京工商大学 Chinese wolfberry fermented raw stock cosmetic and preparation method thereof
CN107998002A (en) * 2017-12-29 2018-05-08 梁立明 A kind of preparation method of plant enzyme facial mask

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674506A (en) * 1994-03-04 1997-10-07 Pacific Corporation Make-up cosmetic compositions containing powdered phaffia yeast
CN104257598A (en) * 2014-09-18 2015-01-07 北京工商大学 Chinese wolfberry fermented raw stock cosmetic and preparation method thereof
CN107998002A (en) * 2017-12-29 2018-05-08 梁立明 A kind of preparation method of plant enzyme facial mask

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Application publication date: 20190104