CN113827521A - Preparation method of tea powder fermentation liquor and application of tea powder fermentation liquor in cosmetics - Google Patents
Preparation method of tea powder fermentation liquor and application of tea powder fermentation liquor in cosmetics Download PDFInfo
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- CN113827521A CN113827521A CN202111208853.0A CN202111208853A CN113827521A CN 113827521 A CN113827521 A CN 113827521A CN 202111208853 A CN202111208853 A CN 202111208853A CN 113827521 A CN113827521 A CN 113827521A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a preparation method of tea powder fermentation liquor and application of the tea powder fermentation liquor in cosmetics. The preparation method comprises the following steps: pulverizing tea, mixing with deionized water, pasteurizing, and fermenting with Lactobacillus plantarum. The probiotic inoculated tea powder is fermented, so that the biological activity of the tea powder is improved, the loss of active ingredients and the emission of harmful substances caused by a chemical extraction method are avoided, and the environment-friendly effect is achieved. The fermentation method adopted by the invention does not add any chemical organic reagent, has mild fermentation conditions and ensures the safety of the fermentation product. The tea powder fermentation liquor prepared by the preparation method disclosed by the invention is high in DPPH free radical clearance rate and can be used as an effective active ingredient in a skin care product.
Description
Technical Field
The invention relates to a preparation method of tea powder fermentation liquor and application of the tea powder fermentation liquor in cosmetics, and belongs to the technical field of biochemistry.
Background
The zinc-selenium organic tea belongs to the ecological health-care tea with pure nature, high quality and no public nuisance. The organic tea is produced without gene engineering, chemical fertilizer, pesticide, growth promoter and other synthetic preparation and radiation technology. Completely follows the natural and ecological principles, and depends on crop straws, waste materials, animal manure, natural green manure and mineral raw materials as much as possible to maintain nutrients. And biological and physical measures are utilized to prevent and control plant diseases and insect pests. Product pollution is prohibited in the packaging and transportation process.
The active substances in the tea mainly comprise tea polysaccharide, tea polyphenol and the like, wherein the tea polyphenol is the most extensive substance in the research of the tea. Tea polyphenol is a general term of polyphenol compounds, accounts for 18-36% of the dry weight of tea leaves, and mainly comprises catechins, flavonoids, anthocyanins and phenolic acids. Wherein the catechin compound accounts for 70-80% of the total amount and is the main component of the secondary metabolites of the tea trees. Catechin is further classified into catechin, epigallocatechin, gallate and epicatechin gallate. Theanine is a non-protein amino acid, accounts for more than 50% of the total amount of amino acids in the tea leaves and accounts for 1% -2% of the dry weight of the tea leaves, and the active ingredients not only provide the biological activity of antioxidation for the tea leaves, but also add the flavor of the tea leaves.
The lactobacillus plantarum is an important probiotic, and can be used for inoculating fermented tea powder to improve the bioactivity potential of the tea powder without destroying nutritional ingredients. Meanwhile, harmful substance residues caused by a chemical extraction residue process are avoided, the utilization rate of active ingredients of tea powder is improved, and the method is environment-friendly.
At present, tea has related applications in the field of cosmetics, and is mainly focused on the extraction and application of tea polyphenol, and the defects that the utilization rate of active ingredients is not high due to the fact that various active ingredients cannot be simultaneously and effectively extracted, and in addition, organic reagent residues are easily generated in the chemical extraction process, and the environmental pollution is easily generated.
Disclosure of Invention
The technical problem solved by the invention is as follows: the prior extraction and application of tea polyphenol have the problems that the utilization rate of active ingredients is not high due to the fact that a plurality of active ingredients cannot be simultaneously and effectively extracted, organic reagent residues are easily generated in the chemical extraction process, and environmental pollution is easily caused.
In order to solve the technical problem, the invention provides a preparation method of tea powder fermentation liquor, which comprises the following steps:
step 1: pulverizing dried folium Camelliae sinensis and sieving to obtain folium Camelliae sinensis powder;
step 2: according to the mass ratio of 1: 30-50, mixing the tea powder obtained in the step 1 with water, and sterilizing at the temperature of 115 ℃ and 121 ℃ for 10-15min to prepare tea powder liquid;
and step 3: pretreatment of zymophyte: streaking lactobacillus plantarum stored at low temperature to obtain single colony, selecting well-grown single colony, inoculating to MRS broth culture medium, culturing at 30-37 deg.C in incubator for 16-20 hr, and inoculating to the tea powder solution obtained in step 2, with inoculation concentration of 106-109CFU/mL mixed solution;
and 4, step 4: culturing the mixed liquor obtained in the step 3 in a constant-temperature incubator for 24-72 hours under a sealed anaerobic condition to obtain fermentation liquor, wherein the fermentation temperature is 30-37 ℃;
and 5: and (4) sterilizing the fermentation liquor obtained in the step (4) by high-pressure steam, centrifuging and taking supernatant fluid to obtain the tea powder fermentation liquor.
Preferably, the tea powder in the step 1 is crushed and then passes through a 80-200 mesh sieve.
Preferably, the rotation speed of the centrifugation in the step 5 is 3000-8000r/min, and the centrifugation time is 10-30 min.
Preferably, the pH value of the tea powder fermentation liquor in the step 5 is 4.0-6.0.
The invention also provides a skin-care product, which comprises the tea powder fermentation liquor prepared by the preparation method of the tea powder fermentation liquor as claimed in any one of claims 1 to 4.
Preferably, the tea powder fermentation liquor is applied as an effective active ingredient in a skin care product.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, probiotics are adopted to ferment tea powder, so that the biological activity of the tea powder is improved, and the problem of active ingredient loss caused by chemical extraction can be avoided;
2. the biological fermentation method adopted by the invention does not add chemical organic reagents, has mild fermentation conditions and can ensure the safety of the fermentation product;
3. the tea powder fermentation liquor provided by the invention has good antioxidant activity and can promote the regeneration of collagen.
Drawings
FIG. 1 is a bar graph showing DPPH free radical scavenging effect of tea powder fermentation broth according to an embodiment of the present invention.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
The Lactobacillus plantarum used in the following examples was Lactobacillus plantarum HH-LP56(Lactobacillus plantarum), which was deposited in the chinese type culture collection with a collection number of CCTCC NO: m2019497, the strain was purchased from West Ann Land Biotech, Inc.
Example 1
Taking 150g of dried tea leaves, crushing, sieving with a 100-mesh sieve, weighing 5g, adding 150mL of water, mixing, and sterilizing at 121 ℃ for 15min to obtain initial tea powder fermentation liquor; streaking Lactobacillus plantarum stored at low temperature to obtain single colony, selecting well-grown single colony, inoculating into MRS broth culture medium, culturing at 30 deg.C in incubator for 20 hr, inoculating into the obtained initial tea powder solution with inoculation concentration of 106CFU/mL mixed solution; culturing the inoculated strain in a constant-temperature incubator for 36h under a sealed anaerobic condition, wherein the fermentation temperature is 30 ℃; sterilizing the fermentation liquor with high pressure steam, centrifuging, and collecting supernatant to obtain the desired tea powder fermentation liquor.
The tea powder fermentation liquor prepared by the embodiment can be used for skin care cosmetics such as astringent and essence.
The tea powder fermentation liquor prepared by the embodiment can also be directly used as toning lotion.
Example 2
Taking 150g of dried tea leaves, crushing, sieving with a 200-mesh sieve, weighing 5g, adding 150mL of water, mixing, and sterilizing at 115 ℃ for 20min to obtain initial tea powder fermentation liquor; streaking lactobacillus plantarum stored at low temperature to obtain single colony, selecting well-grown single colony, inoculating to MRS broth culture medium, culturing at 37 deg.C in incubator for 16 hr, inoculating to the obtained initial tea powder solution with inoculation concentration of 108CFU/mL mixed solution; culturing the inoculated strain in a constant temperature incubator for 24h under a sealed anaerobic condition, wherein the fermentation temperature is 37 ℃; sterilizing the fermentation liquor with high pressure steam, centrifuging, and collecting supernatant to obtain the desired tea powder fermentation liquor.
The tea powder fermentation liquor prepared by the embodiment can be used for skin care cosmetics such as astringent and essence.
The tea powder fermentation liquor prepared by the embodiment can also be directly used as toning lotion.
Example 3
Taking 150g of dried tea leaves, crushing, sieving with a 200-mesh sieve, weighing 5g, adding 150mL of water, mixing, and sterilizing at 120 ℃ for 20min to obtain initial tea powder fermentation liquor; streaking Lactobacillus plantarum stored at low temperature to obtain single colony, selecting well-grown single colony, inoculating to MRS broth culture medium, culturing at 37 deg.C in incubator for 18 hr, inoculating to the obtained initial tea powder solution with inoculation concentration of 107CFU/mL mixed solution; culturing the inoculated strain in a constant temperature incubator for 72 hours under a sealed anaerobic condition, wherein the fermentation temperature is 30 ℃; sterilizing the fermentation liquor with high pressure steam, centrifuging, and collecting supernatant to obtain the desired tea powder fermentation liquor.
The tea powder fermentation liquor prepared by the embodiment can be used for skin care cosmetics such as astringent and essence.
The tea powder fermentation liquor prepared by the embodiment can also be directly used as toning lotion.
Comparative example
Taking 150g of dried tea leaves, crushing, sieving by a 200-mesh sieve, weighing 5g, adding 150mL of water, mixing, and sterilizing at 120 ℃ for 20min to obtain the tea powder liquid.
Analytical experiments:
1. the tea powder fermentation liquors of examples 1 to 3 and the tea powder liquor of the comparative example were subjected to centrifugal filtration, and then the supernatant was subjected to component analysis, and the total phenol content of the tea powder fermentation liquor was analyzed, with the results shown in table 1.
TABLE 1 analysis results of the components of each tea powder fermentation broth
| Sample (I) | Total phenol (mgGAE/mL) |
| Example 1 | 10.14 |
| Example 2 | 9.11 |
| Example 3 | 9.38 |
| Comparative example | 5.49 |
The results of comparing the relevant material components of examples 1-3 with the comparative examples show that the total phenol content after fermentation is obviously improved compared with that before fermentation.
Measurement of DPPH radical scavenging Rate
A tube was charged with 2mL of ethanol DPPH solution (2mM, 75% ethanol) and 2mL of the test solution. Mixing, standing at 25 deg.C in dark for 30min, zeroing with anhydrous ethanol at 517nm wavelength, and measuring absorbance A1. The blank control was prepared by replacing the sample solution with absolute ethanol to determine the absorbance A0, and by replacing the ethanol solution DPPH with an equal volume of absolute ethanol to determine the absorbance A2. The results are shown in table 2 and fig. 1, wherein DPPH radical clearance is calculated according to the following formula:
DPPH radical scavenging capacity ═ a0- (a1-a2)/a0] x 100%.
TABLE 2 determination of DPPH radical scavenging ratio of tea powder fermentation broth
| Sample (I) | Example 1 | Example 2 | Example 3 | Comparative example |
| DPPH radical clearance rate | 95％ | 92％ | 94％ | 86％ |
The results of comparing relevant substance components of examples 1-3 with comparative examples show that the antioxidant activity of the fermented tea powder liquid is obviously improved compared with that before fermentation.
In conclusion, the tea powder fermentation liquor provided by the invention has good antioxidant activity and can be added into cosmetics as an anti-aging additive.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (6)
1. The preparation method of the tea powder fermentation liquor is characterized by comprising the following steps:
step 1: pulverizing dried folium Camelliae sinensis and sieving to obtain folium Camelliae sinensis powder;
step 2: according to the mass ratio of 1: 30-50, mixing the tea powder obtained in the step 1 with water, and sterilizing at the temperature of 115 ℃ and 121 ℃ for 10-15min to prepare tea powder liquid;
and step 3: pretreatment of zymophyte: streaking lactobacillus plantarum stored at low temperature to obtain single colony, selecting well-grown single colony, inoculating to MRS broth culture medium, culturing at 30-37 deg.C in incubator for 16-20 hr, and inoculating to the tea powder solution obtained in step 2, with inoculation concentration of 106-109CFU/mL mixed solution;
and 4, step 4: culturing the mixed liquor obtained in the step 3 in a constant-temperature incubator for 24-72 hours under a sealed anaerobic condition to obtain fermentation liquor, wherein the fermentation temperature is 30-37 ℃;
and 5: and (4) sterilizing the fermentation liquor obtained in the step (4) by high-pressure steam, centrifuging and taking supernatant fluid to obtain the tea powder fermentation liquor.
2. The method for preparing tea powder fermentation liquor according to claim 1, wherein the tea powder in the step 1 is crushed and then passed through a 80-200 mesh sieve.
3. The method for preparing tea powder fermentation broth according to claim 1, wherein the centrifugation speed in step 5 is 3000-8000r/min, and the centrifugation time is 10-30 min.
4. The method for preparing tea powder fermentation broth according to claim 1, wherein the pH of the tea powder fermentation broth in step 5 is 4.0-6.0.
5. A skin care product comprising the tea powder fermentation broth prepared by the method for preparing tea powder fermentation broth according to any one of claims 1 to 4.
6. The skin care product of claim 5, wherein the tea powder fermentation broth is used as an active ingredient in a skin care product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601761A (en) * | 2022-01-18 | 2022-06-10 | 科丝美诗(中国)化妆品有限公司 | Preparation method and application of plant fermentation liquor for repairing skin barrier |
| CN115637193A (en) * | 2022-11-24 | 2023-01-24 | 湖南金浩粮油工业有限公司 | Refining process of tea oil for cosmetics |
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| CN105250168A (en) * | 2015-09-30 | 2016-01-20 | 上海全丽生物科技有限公司 | High mountain tea fermentation raw stock cosmetic and preparation method and application thereof |
| CN111616997A (en) * | 2020-06-05 | 2020-09-04 | 上海全丽生物科技有限公司 | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof |
| CN112870130A (en) * | 2021-03-30 | 2021-06-01 | 中国科学院合肥物质科学研究院 | Preparation method of eucommia pollen fermentation liquor and application of eucommia pollen fermentation liquor in cosmetics |
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2021
- 2021-10-18 CN CN202111208853.0A patent/CN113827521A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105250168A (en) * | 2015-09-30 | 2016-01-20 | 上海全丽生物科技有限公司 | High mountain tea fermentation raw stock cosmetic and preparation method and application thereof |
| CN111616997A (en) * | 2020-06-05 | 2020-09-04 | 上海全丽生物科技有限公司 | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof |
| CN112870130A (en) * | 2021-03-30 | 2021-06-01 | 中国科学院合肥物质科学研究院 | Preparation method of eucommia pollen fermentation liquor and application of eucommia pollen fermentation liquor in cosmetics |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601761A (en) * | 2022-01-18 | 2022-06-10 | 科丝美诗(中国)化妆品有限公司 | Preparation method and application of plant fermentation liquor for repairing skin barrier |
| CN115637193A (en) * | 2022-11-24 | 2023-01-24 | 湖南金浩粮油工业有限公司 | Refining process of tea oil for cosmetics |
| CN115637193B (en) * | 2022-11-24 | 2024-05-14 | 湖南金浩粮油工业有限公司 | Refining process of tea oil for cosmetics |
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