KR101815925B1 - Fermentated Composition of Barley Sprouts Dnhanced Policosanol and Method For Composition Thereof - Google Patents
Fermentated Composition of Barley Sprouts Dnhanced Policosanol and Method For Composition Thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
본 발명은 식품분의 기술로써, 보리새싹의 혈행개선 효능을 갖는 폴리코사놀을 증가시킨 보리새싹 발효 추출물 및 그의 제조방법에 관한 것이다.The present invention relates to a fermented barley bud extract having increased policosanol having a blood circulation improving effect of barley sprouts as a food ingredient, and a process for producing the same.
Description
본 발명은 식품분야의 기술로써, 보다 상세게는 보리새싹의 혈행개선 효능을 갖는 폴리코사놀을 증가시킨 보리새싹 발효 추출물 및 그의 제조방법에 관한 것이다.The present invention relates to a fermented barley seed extract having increased policosanol having a blood circulation improving effect on barley sprouts as a technology in the food field, and a process for producing the fermented barley germ extract.
근래에 들어 많은 새싹식물들의 기능적인 측면이 대두되면서 새싹식물에 대한 관심이 높아지고 있는 추세이다. 그 중 보리새싹의 효능은 이미 오래전부터 알려져 있었으며, 1931년에 미국의 식품 연구가인 찰스 슈나벨은 닭모이를 연구하다가 보리새싹으로 우수한 모이를 개발하였으며, 그 모이를 먹은 닭은 겨울에도 계란의 생산량이 150%나 증가하였다. 그 후 많은 부분에서 새싹에 대한 연구가 지속되었는데, 1930년대 후반부터 합성 제조한 각종 비타민이 등장하면서 뒤로 밀려나 버렸다. 이러한 새싹식물에 대한 연구는 근래에 들어서 다시 대두되고 있다.In recent years, as the functional aspect of many bud plants has emerged, interest in bud plants has been increasing. Among them, the efficacy of barley sprouts has been known for a long time, and in 1931 Charles Schnabel, a food researcher in the US, studied barnyard grass and developed excellent barley buds. 150%. Since then many studies on buds have continued, but since the late 1930s, a variety of synthetic vitamins have been introduced and pushed back. Studies on these sprout plants have emerged again in recent years.
보리새싹은 70%가 엽록소인데, 산소가 풍부하여 체내의 독소를 제거하고 염증을 완화시키며 세포의 돌연변이를 막아주고 상처를 회복시켜주는 효과가 있으며, 항암물질인 SOD, P401, 셀렌, 레트릴이 함유되어 있다. 또한, 보리새싹은 10가지가 넘는 비타민, 20가지나 되는 아미노산과 많은 메네랄 및 효소를 함유하고 있는데, 특히 식물이 가뭄과 같은 스트레스를 받으면 합성되는 아브시스산을 함유하고 있으며, 새싹보리의 폴리코사놀이란 성분이 몸에 유익한 콜레스테롤의 수치는 올리고, 해로운 콜레스테롤의 수치를 낮추는 효과를 나타내 중자년층의 건강기능성 식품으로 선호되고 있다.Barley buds are chlorophyll, 70% of which is rich in oxygen, which removes toxins in the body, alleviates inflammation, inhibits cell mutation and restores wounds, and contains anti-cancer substances such as SOD, P401, selenium and retrile . Barley sprouts contain more than 10 vitamins, 20 amino acids and a lot of menerals and enzymes. Especially when plants are stressed like drought, they contain abscisic acid, and policosanol Ingredients have beneficial effects on the body, such as elevated levels of cholesterol, lowers the levels of harmful cholesterol, and is preferred as a healthy functional food for middle-aged people.
상기 폴리코사놀(Policosanol)은 식물 왁스에서 추출한 천연 지방 알코올 추출물들을 총칭하는 이름으로써, 보통 저밀도 지질단백질(Low density cholesterol)을 낮추고 고밀도 지질단백질(High density cholesterol)을 높여준다는 효과가 있는 것으로 알려져 동맥경화를 방지하기 위한 영양 보충제로 사용되고 있으며, 이러한 보리새싹의 폴리코사놀을 추출하는 방법과 폴리코사놀을 증대시키기 위한 연구는 계속되고 있다.Policosanol is a generic term for natural fatty alcohol extracts extracted from plant wax. It is known that Policosanol lowers low density lipolytic protein and increases high density lipoprotein (high density cholesterol). And policosanol has been used as a nutritional supplement to prevent policosanol. Polycosanol has been extensively studied for the purpose of increasing policosanol.
한국등록특허 제10-1483589호는 새싹보리 추출물 및 이로부터 분리된 폴리코사놀계 화합물을 포함하는 대사성 질환 예방 및 치료용 약학 조성물에 관한 발명이며, 한국등록특허 제10-1540737호는 폴리코사놀 함량이 증가된 새싹밀, 새싹귀리 또는 새싹 호밀 추출물의 제조방법으로써, 두 발명은 새싹으로부터 폴리코사놀을 추출하는 방법에 관한 것으로, 이는 본원발명과 구성이 다르다.Korean Patent No. 10-1483589 is an invention relating to a pharmaceutical composition for preventing and treating metabolic diseases comprising shoot barley extract and a policosanol-based compound isolated therefrom, and Korean Patent No. 10-1540737 discloses a pharmaceutical composition for preventing and treating metabolic diseases, The present invention relates to a method of extracting policosanol from buds, which differs in composition from the present invention.
본 발명은 상기와 같은 문제점을 해결하기 위해 안출한 것으로써, 본 발명의 목적은 폴리코사놀을 증대된 보리새싹 발효 추출물을 제공하는 데 있다.DISCLOSURE Technical Problem The present invention has been made to overcome the above-mentioned problems, and it is an object of the present invention to provide policosanol-enhanced barley bud fermentation extract.
아울러, 본 발명의 목적은 상술한 폴리코사놀을 증대된 보리새싹 발효 추출물의 제조방법을 제공하는데 있다.In addition, it is an object of the present invention to provide a method for producing policosanol-enhanced barley bud fermentation extract described above.
본 발명은 상술한 첫 번째 기술적 과제를 해결하기 위하여 폴리코사놀이 증대된 보리새싹 발효 추출물을 제공한다.In order to solve the above-mentioned first technical problem, the present invention provides policosanol-enhanced barley bud fermentation extract.
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한편, 본 발명은 상술한 두 번째 기술적 과제를 해결하기 위하여, 폴리코사놀이 증대된 보리새싹 발효 추출물의 제조 방법으로서, (a). 보리새싹을 락토바실러스 불가리쿠스로 42℃에서 72시간 동안 발효시키는 단계; (b). 상기 (a)단계의 발효물을 동결건조시키는 단계; (c). 상기 (b) 단계의 동결건조된 발효물을 헥산 용매를 사용하여 70℃에서 6시간 동안 추출하는 단계; (d). 상기 (c)단계의 추출물을 분말화 하는 단계를 포함하는, 폴리코사놀이 증대된 보리새싹 발효 추출물의 제조 방법을 제공한다.According to another aspect of the present invention, there is provided a method for producing policosanol-enhanced fermented barley buds, comprising the steps of: (a) Fermenting barley sprouts with Lactobacillus bulgaricus at 42 DEG C for 72 hours; (b). Lyophilizing the fermented product of step (a); (c). Extracting the lyophilized fermented product of step (b) with a hexane solvent at 70 DEG C for 6 hours; (d). And powdering the extract of step (c). The present invention also provides a method for producing policosanol-fermented barley bud fermentation extract.
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본 발명에 따르는 폴리코사놀이 증대된 보리새싹 발효 추출물은 특정 균주로 보리새싹을 발효시킨 후 추출함으로써 폴리코사놀이 다량 함유된 발효 추출물을 제공함으로써 식의약, 화장품의 소재 및 제품에 활용할 수 있다.The policosanol-enriched fermented extract of policosanol according to the present invention can be utilized in foods and cosmetics by providing a fermented extract containing a large amount of policosanol by fermenting barley sprouts as a specific strain and extracting it.
도 2는 본 발명의 다른 실시예에 따른 유산균 발효 보리새싹 추출물과 비 발효 추출물의 폴리코사놀 함량을 비교한 그래프이다.
도 3은 본 발명의 또 다른 실시예에 따른 효소 처리에 따른 보리새싹의 폴리코사놀 함량을 비교한 그래프이다.
도 4는 본 발명의 또 다른 실시예에 따른 보리새싹 추출물에 cyclodextrin의 첨가농도에 따른 분말화를 나타낸 그림이다.2 is a graph comparing policosanol contents of a fermented barley sprout extract with a non-fermented extract according to another embodiment of the present invention.
FIG. 3 is a graph comparing policosanol contents of barley sprouts according to an enzyme treatment according to another embodiment of the present invention.
FIG. 4 is a graph showing pulverization according to the concentration of cyclodextrin added to barley bud extract according to another embodiment of the present invention. FIG.
이하 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
본 발명에서 사용되는 기술적 용어는 단지 특정한 실시 예를 설명하기 위해 사용된 것으로, 본 발명을 한정하려는 의도가 아님을 유의해야 한다. 또한, 본 발명에서 사용되는 기술적 용어는 본 발명에서 특별히 다른 의미로 정의되지 않는 한, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 의미로 해석되어야 하며, 과도하게 포괄적인 의미로 해석되거나, 과도하게 축소된 의미로 해석되지 않아야 한다. 또한, 본 발명에서 사용되는 기술적인 용어가 본 발명의 사상을 정확하게 표현하지 못하는 잘못된 기술적 용어일 때에는, 당업자가 올바르게 이해할 수 있는 기술적 용어로 대체되어 이해되어야 할 것이며, 본 발명에서 사용되는 일반적인 용어는 사전에 정의되어 있는 바에 따라, 또는 전후 문맥상에 따라 해석되어야 하며, 과도하게 축소된 의미로 해석되지 않아야 하고, 본 발명에서 사용되는 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한 복수의 표현을 포함하며, 본 발명에서 추가적인 구성 요소 또는 단계들을 더 포함할 수 있는 것으로 해석되어야 하며, 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.It is noted that the technical terms used in the present invention are used only to describe specific embodiments and are not intended to limit the present invention. In addition, the technical terms used in the present invention should be construed in a sense generally understood by a person having ordinary skill in the art to which the present invention belongs, unless otherwise defined in the present invention, Should not be construed to mean, or be interpreted in an excessively reduced sense. In addition, when a technical term used in the present invention is an erroneous technical term that does not accurately express the concept of the present invention, it should be understood that technical terms that can be understood by a person skilled in the art are replaced with a technical term. And should not be construed as being excessively reduced in meaning, and the singular expressions used in the present invention include plural expressions unless the context clearly dictates otherwise. ≪ RTI ID = 0.0 > And it is to be understood that the present invention may be embodied with additional constituent elements or steps. In the following description of the present invention, a detailed description of related arts will be omitted. It is omitted.
이하, 첨부된 도면을 참조하여 본 발명에 따른 바람직한 실시예를 상세히 설명하되, 도면 부호에 관계없이 동일하거나 유사한 구성 요소는 동일한 참조 번호를 부여하고 이에 대한 중복되는 설명은 생략하기로 한다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, wherein like reference numerals refer to like or similar elements throughout the several views, and redundant description thereof will be omitted.
본 발명은 상술한 첫 번째 기술적 과제를 해결하기 위하여 폴리코사놀이 증대된 보리새싹 발효 추출물을 제공한다.In order to solve the above-mentioned first technical problem, the present invention provides policosanol-enhanced barley bud fermentation extract.
본 발명의 일 실시예에 의하면, 상기 보리새싹 발효 조성물은 유산균에 의하여 발효된 것일 수 있다.According to one embodiment of the present invention, the barley bud fermentation composition may be fermented by lactic acid bacteria.
한편, 본 발명은 상술한 두 번째 기술적 과제를 해결하기 위하여, 폴리코사놀이 증대된 보리새싹 발효 추출물의 제조 방법으로서, (a). 보리새싹을 락토바실러스 불가리쿠스로 42℃에서 72시간 동안 발효시키는 단계; (b). 상기 (a)단계의 발효물을 동결건조시키는 단계; (c). 상기 (b) 단계의 동결건조된 발효물을 헥산 용매를 사용하여 70℃에서 6시간 동안 추출하는 단계; (d). 상기 (c)단계의 추출물을 분말화 하는 단계를 포함하는, 폴리코사놀이 증대된 보리새싹 발효 추출물의 제조 방법을 제공한다.According to another aspect of the present invention, there is provided a method for producing policosanol-enhanced fermented barley buds, comprising the steps of: (a) Fermenting barley sprouts with Lactobacillus bulgaricus at 42 DEG C for 72 hours; (b). Lyophilizing the fermented product of step (a); (c). Extracting the lyophilized fermented product of step (b) with a hexane solvent at 70 DEG C for 6 hours; (d). And powdering the extract of step (c). The present invention also provides a method for producing policosanol-fermented barley bud fermentation extract.
본 발명의 다른 실시예에 의하면, 상기 발효단계의 유산균은 Lactobacillus bulgaricus 및 Streptococcus thermophilus로 이루어진 군으로부터 선택되는 것이 바람직하고 Lactobacillus bulgaricus인 것이 표 1에 나타난 바와 같이 폴리코사놀 함량 증대 효과에 있어 더욱 바람직하다.According to another embodiment of the present invention, the lactic acid bacterium in the fermentation step is preferably selected from the group consisting of Lactobacillus bulgaricus and Streptococcus thermophilus , and Lactobacillus bulgaricus is more preferable for enhancing policosanol content as shown in Table 1.
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이하, 하기 실시예, 비교예 및 실험예를 통하여 본 발명의 구성 및 효과를 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것일 뿐, 본 발명의 범위가 이들 실시예에 의해 제한되는 것은 아니다.Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to the following examples, comparative examples and experimental examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.
<< 실험예Experimental Example 1> 유산균1> lactic acid bacteria 발효 샘플의 준비 Preparation of fermentation samples
1.1 실험에 사용한 보리새싹의 준비1.1 Preparation of barley buds used in the experiment
보리새싹은 2015년 8월 초에 수확(15 cm내외와 20 cm내외)한 보리새싹으로써, 저온 열풍 건조를 통해 건조시킨 보리새싹을 구매 (영농조합법인 애농, 진안)하여 사용하였다. 건조된 보리새싹을 분쇄기(Hanil Food Mixer HMF-3000S, Korea)로 분쇄한 후 25 mesh 체에 통과시켜 밀봉 팩에 넣어 보관하면서 실험에 사용하였다. 보리새싹 원료는 본 연구에 사용된 것에만 국한 되지 않는다. Barley sprouts were harvested (about 15 cm and about 20 cm) in early August 2015. Barley sprouts were dried and purchased through low temperature hot air drying (Agonin and Jinan). The dried barley sprouts were pulverized with a grinder (Hanil Food Mixer HMF-3000S, Korea), passed through a 25-mesh sieve, and stored in a sealed pack for storage. Barley bud raw materials are not limited to those used in this study.
1.2 유산균 발효 보리새싹 헥산추출물의 제조방법 1.2 Preparation method of fermented barley sprout hexane extract of lactic acid bacteria
15 cm내외와 20 cm내외의 보리새싹 건조 파우더 (>25 mesh) 5%를 30 ml (w/v)을 만든 후 autoclave를 이용하여 멸균(121℃, 15분)을 시켰다. 멸균시킨 용액에 각 유산균을 1%씩 접종하였다. 유산균을 접종한 보리새싹은 40 ℃에서 72시간동안 발효시킨 후 동결건조 하여 실험에 사용하였다. 30 ml (w / v) of 5% of barley sprout dried powder (> 25 mesh) around 15 cm and 20 cm was sterilized (121 ° C, 15 minutes) using an autoclave. The sterilized solution was inoculated with 1% of each lactic acid bacteria. The barley buds inoculated with lactic acid bacteria were fermented at 40 ℃ for 72 hours and then lyophilized and used in the experiment.
동결건조가 끝난 발효물 0.5g를 보리새싹으로부터 폴리코사놀 추출을 위한 용매로 n-Hexane 추출 방법을 이용하여 보리새싹 유산균 발효물을 추출한 후 시험에 사용하였다. 25 mesh의 sieve에 거른 새싹보리 분말 100 g을 둥근 바닥 플라스크에 넣고, n-Hexane 2 L를 가한 뒤, 70 ℃로 맞춘 항온수조에 고정시켜 Hexane추출을 수행하였다. 6시간 후 추출액을 감압 여과(여과지 ADVANTEC Filter Paper No. 2)한 뒤 n-Hexane 1 L를 추가로 가해 3시간을 더 추출하고 다시 감압농축을 한 후 동결건조를 수행하였다. 보리새싹을로부터 폴리코사놀 추출을 위한 용매로 핵산만 국한하지 않고 물, 알코올 등 모두 유기용매 추출이 가능 하다. 0.5 g of the lyophilized fermented product was used as a solvent for policosanol extraction from barley sprouts, and the fermented product of barley sprout lactobacillus was extracted using n-Hexane extraction method. 100 g of the dried barley powder was poured into a round-bottomed flask, and 2 L of n-hexane was added thereto. Then, the mixture was fixed in a constant-temperature water bath set at 70 ° C. to perform hexane extraction. After 6 hours, the extract was filtered under reduced pressure (filter paper ADVANTEC Filter Paper No. 2), and further 1 L of n-hexane was added thereto for further 3 hours. The filtrate was concentrated under reduced pressure and freeze-dried. It is possible to extract organic solvents from water, alcohol and so on without extracting only nucleic acid from the barley sprout as a solvent for policosanol extraction.
<< 실험예Experimental Example 2> 효소처리 샘플의 준비 2> Preparation of enzyme-treated sample
보리새싹 분말 100 g에 증류수 1 L를 넣은 뒤 각 분말상태의 효소 1 g(셀룰레이즈와 리파아제)을 넣고 37 ℃ incubator에서 150 rpm으로 2시간 동안 교반하면서 반응시킨 후 감압여과 후 잔사물을 헥산으로 3시간 동안 추출하여 폴리코사놀 함량을 분석하였다.1 g of distilled water was added to 100 g of barley sprout powder, and 1 g of enzyme in each powder state (cellulase and lipase) was added. The mixture was reacted at 37 ° C in an incubator at 150 rpm for 2 hours with stirring. After filtration under reduced pressure, Policosanol content was analyzed by extraction for 3 hours.
<실험예3> Cyclodextrin의 첨가Experimental Example 3: Addition of Cyclodextrin
Emulsion 상태의 보리새싹 핵산추출 동결건조물 10%를 증류수에 첨가한 후 여기에 cyclodextrin 5%와 10%를 첨가하고 37℃ incubator에서 3시간 동안 교반시킨 다음 동결건조하였다. After adding 10% of the lyophilized extract of barley sprout extracted with emulsion to distilled water, 5% and 10% of cyclodextrin were added thereto, and the mixture was stirred at 37 ° C for 3 hours and then lyophilized.
<< 시험예Test Example 1> 유산균 발효 1> Fermentation of lactic acid bacteria 보리새싹Barley bud 추출물의 Extract 폴리코사놀Policosanol 분석 analysis
유산균 발효 보리새싹 헥산추출물을 GC로 분석하였다.Lactic acid fermented barley sprout hexane extract was analyzed by GC.
유산균 발효 보리새싹 헥산추출물의 GC 분석 결과에 다른 폴리코사놀 함량은 하기 표 1과 도 2와 같았다.GC analysis of lactic acid fermented barley sprout hexane extract showed other policosanol contents as shown in Table 1 and FIG. 2 below.
도 2는 본 발명의 다른 실시예에 따른 유산균 발효 보리새싹 추출물과 비 발효 추출물의 폴리코사놀 함량을 비교한 그래프이다. 2 is a graph comparing policosanol contents of a fermented barley sprout extract with a non-fermented extract according to another embodiment of the present invention.
표 1 및 도 2를 살펴보면 비발효 보리새싹의 폴리코사놀 함량보다 유산균 발효를 시킨 보리새싹의 함량이 더 높다는 것을 확인할 수 있었다. 또한 S. thermophilus로 발효시킨 보리새싹보다 L. bulgaricus로 발효시킨 보리새싹이 폴리코사놀 함량이 더 높다는 것을 확인할 수 있었다. L. bulgaricus 발효물이 비발효물인 control(15 cm 보리새싹, 391.33 mg/100 g; 20 cm 보리 새싹, 465.50 mg/100 g)보다 폴리코사놀 함량이 약 2배 정도 높게 검출되었고, 15 cm 내외 보리새싹 L. bulgaricus 발효물 (BS-15-L)(826.02 mg/100 g)보다 20 cm 내외 보리새싹 L. bulgaricus 발효물 (BS-20-L)의 함량(935.82 mg/100 g)이 약 1.1배 정도 높게 나왔음을 확인할 수 있었다. Table 1 and FIG. 2 show that the content of barley sprouts fermented with lactic acid bacteria is higher than the policosanol content of non-fermented barley sprouts. The barley sprouts fermented with L. bulgaricus showed higher policosanol content than barley sprouts fermented with S. thermophilus . L. bulgaricus Policosanol content was about twice as high as that of control (15 cm barley sprout, 391.33 mg / 100 g; 20 cm barley sprout, 465.50 mg / 100 g) and about 15 cm inside and outside barley sprout L. bulgaricus The content (935.82 mg / 100 g) of L. bulgaricus fermented product (BS-20-L) was about 1.1 times higher than that of fermented product (BS-15-L) (826.02 mg / 100 g) .
폴리코사놀이 가장 많이 분석되어진 L. bulgaricus 로 발효되어진 20 cm 보리새싹으로부터 폴리코사놀의 각 성분을 발효전과 후로 비교해볼 때, docosanol은 발효전에 비해 약 2.5배, tetracosanol은 약 2.3배, hexacosanol은 약 2.0배, heptacosanol은 약 2.5배, octacosanol은 약 2.4배 그리고 triacontanol은 약 2배가 증가하였다.When policosanol was inoculated from 20 cm barley sprouts fermented with L. bulgaricus , the content of docosanol was about 2.5 times higher than before fermentation, tetracosanol was about 2.3 times higher than before fermentation, hexacosanol was about 2.0 times higher than that before fermentation, heptacosanol was about 2.5 times, octacosanol was about 2.4 times, and triacontanol was about 2 times.
<< 시험예Test Example 2> 2> 보리새싹Barley bud 효소처리에 따른 Following enzyme treatment 폴리코사놀Policosanol 함량 분석 Content analysis
도 3은 본 발명의 또 다른 실시예에 따른 효소 처리에 따른 보리새싹의 폴리코사놀 함량을 비교한 그래프이다.FIG. 3 is a graph comparing policosanol contents of barley sprouts according to an enzyme treatment according to another embodiment of the present invention.
도 2를 살펴보면, 유산균 발효 이외 효소 처리에 따른 보리새싹의 폴리코사놀 함량 비교 결과는 그림 3에 나타냈다. 추출물 기준으로 control로 사용한 보리새싹 3시간 헥산 추출물의 폴리코사놀 함량은 437.60 mg/100 g, cellulase를 처리한 3시간 헥산 추출물은 468.78 mg/100 g, lipase를 처리한 3시간 헥산 추출물은 398.08 mg/100 g이 검출되었다. 3개의 시료의 폴리코사놀 함량이 큰 차이가 없고 cellulase 처리구는 control보다 높은 함량이, lipase 처리구는 control보다 낮은 함량이 검출되었다. 2, the results of the comparison of the policosanol content of the barley bud according to the enzymatic treatment except lactic acid bacteria fermentation are shown in FIG. The content of policosanol was 437.60 mg / 100 g, 3 hours, 468.78 mg / 100 g, and 3 hours, respectively. The extracts of 3 hours and 388.08 mg / 100 g of the lipase- g was detected. There was no significant difference in policosanol content among three samples. Cellulase treatment showed higher content than control and lipase treatment had lower content than control.
<< 시험예Test Example 3> 3> 폴리코사놀이Policosanol 함유된 Contained 보리새싹Barley bud 추출물의 Extract 분말화Powdered
도 4는 본 발명의 또 다른 실시예에 따른 보리새싹 추출물에 cyclodextrin의 첨가농도에 따른 분말화를 나타낸 그림이다.FIG. 4 is a graph showing pulverization according to the concentration of cyclodextrin added to barley bud extract according to another embodiment of the present invention. FIG.
도 4를 살펴보면 보리새싹 헥산추출물은 분말 상태가 아닌 emulsion 형태이며 이러한 폴리코사놀이 함유된 보리새싹 추출물을 cyclodextrin을 이용하여 분말화 하였으며 결과는 그림 4에 나타냈다. Cyclodextrin 무첨가구는 입자가 곱지 않고 뭉쳐있으며 만지면 달라붙는 emulsion 형태를 기준으로 cyclodextrin을 첨가하면 분말화가 진행됨을 알 수 있었고, 10% cyclodextrin 첨가 했을 때 완전히 분말화가 이루어짐을 확인할 수 있었다. As shown in FIG. 4, the extract of barley sprout hexane was in an emulsion form, not a powder state. The extract of barley sprouts containing policosanol was pulverized using cyclodextrin. The results are shown in FIG. The addition of cyclodextrin on the basis of the emulsion type, which was clumped without any addition of the cyclodextrin, showed progressive pulverization, and the addition of 10% cyclodextrin showed complete pulverization.
<< 실시예Example 1> 산업적 생산을 위한 유산균 스타터를 이용한 1> Using lactic acid bacteria starter for industrial production 보리새싹의Barley bud 발효 Fermentation
보리새싹 발효 유산균 스타터를 이용하여 40 ℃에서 72시간동안 발효시킨 후 동결건조 하여 헥산 추출 농축 전처리 과정 없이 시중에 판매되는 제품과 비교 분석한 결과를 하기 표 2에 나타내었다. 각각의 유산균을 사용하여 발효 했을 때와 같이 유산균 스타터를 사용하여 발효시켰을 때도 비발효 보리새싹보다 폴리코사놀 함량이 증가함을 확인할 수 있었다. 각각의 유산균을 사용하여 발효된 보리새싹의 폴리코사놀 함량 보다 적게 분석된 것은 헥산 추출을 따로 하지 않았기 때문에 함량이 상대적으로 적게 검출되었다. YC-X11로 발효한 보리새싹은 control(C)과 비교했을 때 폴리코사놀 함량이 높아 졌으며, 기능성을 가지고 있는 hexacosanol과 octacosanol이 증가된 것을 확인 할 수 있었다. The fermented product was fermented at 40 ° C for 72 hours using barley germicide fermented lactic acid bacteria starter, followed by lyophilization and hexane extract concentration without pretreatment. The results are shown in Table 2 below. When fermented with lactic acid bacteria starter, it was confirmed that policosanol content was higher than that of non fermented barley sprout. The content of policosanol in barley sprouts fermented with each lactic acid bacterium was lower than that of policosanol because the hexane extraction was not performed. The barley buds fermented with YC-X11 showed higher policosanol content than control (C), and hexacosanol and octacosanol, which have functional properties, were increased.
우리나라 4대 곡물 의 하나인 보리를 이용하여 겨울철 비어있는 농지를 활용하는 효과를 나타낼 수 있으며, 부가가치가 높은 보리를 재배하며, 폴리코사놀의 함량을 증대시킴으로써 기능성 식품소재로써의 활용도를 높일 수 있다.Using barley, one of the four major grains of Korea, it is possible to utilize vacant farmland in winter, cultivate high value - added barley, and increase the content of policosanol, so that it can be used as a functional food material.
삭제delete
Claims (7)
(b). 상기 (a)단계의 발효물을 동결건조시키는 단계;
(c). 상기 (b) 단계의 동결건조된 발효물을 헥산 용매를 사용하여 70℃에서 6시간 동안 추출하는 단계;
(d). 상기 (c)단계의 추출물을 분말화 하는 단계를 포함하는, 폴리코사놀이 증대된 보리새싹 발효 추출물의 제조 방법.(a). Fermenting 20 cm of barley sprouts with Lactobacillus bulgaricus at 42 DEG C for 72 hours;
(b). Lyophilizing the fermented product of step (a);
(c). Extracting the lyophilized fermented product of step (b) with a hexane solvent at 70 DEG C for 6 hours;
(d). , And powdering the extract of step (c). The policosanol-fermented extract of fermented barley germ.
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| KR102455930B1 (en) | 2019-10-21 | 2022-10-17 | 엄화자 | Process for preparing fermented barley grain and the use thereof |
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