KR20230001019A - Hydroponic cultivation method of barley sprouts dnhanced policosanol and barley sprouts prepared thereby - Google Patents

Abstract

The present invention relates to a hydroponic cultivation method of barley sprouts in which policosanol is increased and barley sprouts grown thereby. The hydroponic cultivation method of barley sprouts comprises the steps of: (A) preparing barley sprout seeds on a tray and supplying a nutrient solution so that a part of the barley sprout seeds located on the tray is immersed; (B) spraying bubbles on the barley sprout seeds in contact with purified water; and (C) irradiating LED light to the barley sprout seeds in contact with purified water for 1 to 10 hours. The method can increase the content of policosanol, which is effective for alleviating hyperlipidemia and diabetes, improving blood circulation, and alleviating hangovers and alcoholic fatty liver disease.

Description

Hydroponic cultivation method of barley sprouts dnhanced policosanol and barley sprouts prepared thereby

The present invention relates to a hydroponic cultivation method of barley sprouts capable of increasing the content of policosanol, and to barley sprouts grown according to the method.

Society is aging due to the development of medical technology in modern society, awareness of health is spreading due to economic development, and the pursuit of high quality of life has become a trend not only in Korea but also worldwide predicted to be Therefore, it is necessary to develop new health care materials, functional food materials, and well-being materials that can meet rapidly changing social phenomena and increasingly diversified consumer expectations.

Barley sprout (young barley leaf) has been known as a nutritionally excellent food source for its high content of inorganic components such as calcium, magnesium and potassium, as well as high content of vitamin B1 and vitamin C (Korean Patent Publication No. 2004- 0045798, barley leaf tea manufacturing method, Kim Dong-won et al.) In addition, it contains functional secondary metabolites such as Saponarin, Lutonarin, and Isovitexin, which have antioxidant, anti-inflammatory, and anti-cancer functions, Its industrial applicability as a food and pharmaceutical material is increasing. (Benedet JA et al, J. Agric. Food. Chem, 2007, 55, 5499-5504)

In Japan and the United States, barley sprouts are dried and powdered products are developed and sold as health foods.

On the other hand, Policosanol is known as a substance extracted from sugar cane (Saccharum Officinarium) or beeswax, and is a mixture of long carbon chain primary saturated aliphatic alcohols. Its composition is Eicosanol (C20H42O), Heneicosanol (C21H44O), Docosanol (C22H46O), Tricosanol (C23H48O), Tetracosanol (C24H50O), Hexacosanol (Hexacosanol, C26H54O), Heptacosanol (C27H56O), Octacosanol (C28H58O), and Triacontanol (C30H62O) components are collectively called, and there is no independent chemical formula. Among them, patents and reports related to hexacosanol and octacosanol that have been published until recently inhibit the activity of HMG-CoA reductase, an enzyme that catalyzes the initial rate-limiting step in cholesterol synthesis, thereby reducing total cholesterol and low-density low-protein cholesterol (LCL-C). There is a report (Oh Han-jin, Food Industry and Nutrition, 15(1), 24-26, 2010) that it lowers the level.

In addition, elderly patients with hypertension and type 2 hypercholesterolemia treated with policosanol isolated from sugar cane at a dose of 20 mg/day for 12 months showed significantly reduced total cholesterol (TC) and low-density cholesterol (LDL-C) levels. , and there are reports of clinical studies that increase the level of high-density cholesterol (HDL-C) (Castano et al, Int. J. Clin Pharmacol, 2001, 21, 43-57; Gouni-Berthold et al, American Heart

Journal, 2002, 143(2), 356-365).

In addition, among policosanols, hexacosanol has the effect of regulating strepdozotocin-induced blood sugar, prevents the increase of TGF-beta1 and malondialdehyde in the renal organ, and regulates the expression of nitric oxide synthase. There are also research reports that it is effective in suppressing type of diabetes and obesity (Saito et al, European Journal of Phamacology, 2006, 544, 132-137; Okada et al, Mol Cell Biochem, 2008, 315, 169-177).

In addition, compositions of policosanols such as octacosanol and hexacosanol have been demonstrated to exhibit anti-thrombotic activity with significant inhibition of platelet aggregation (Carbajal et al, pharmacol. Res., 1998, 38, 89-91), energy It is an activating factor that releases , which generates energy in muscles and the human body (Incheol So, Journal of Exercise Nutrition, 2006, 10(3), 275-280), while increasing cell activity through increased ATP production, resulting in skin wrinkles and elasticity. It was confirmed that it has various functions, such as reporting that it is greatly improved (Republic of Korea Patent Publication No. 2008-0119520).

Patents and reports related to barley sprouts published until recently include an extract containing barley sprout-derived polyphenolic compounds and a method for preparing the same (Korean Patent Publication No. 2010-0005714), inhibition of neuraminidase activity containing barley sprout extract composition and pharmaceutical composition for preventing and treating influenza virus infectious diseases (Korean Patent Publication No. 2011-0020660), wheatgrass or barley sprout tea manufacturing method (Korean Patent Publication No. 2006-0072848), beverage for diabetic patients (Korean Publication) Patent No. 2006-0089639), analysis of water-soluble phenolic compounds in barley leaves (Duh PD et al, J. Agric. Food Chem, 2001, 49, 1455-1463), tofu containing barley leaf powder (Korean Patent Publication No. 2007-0122846), etc. In addition, a study on the cholesterol improvement effect using barley sprouts was conducted on the effect of barley sprouts on lipid content and enzymatic activity related to liver tissue lipid metabolism in mice fed high fat (Yang Eun-joo et al., Journal of the Korean Society of Nutrition, 2009, 42(1), 14-22 ) and a study on the antioxidant activity and hyperlipidemia inhibitory effect of barley sprout extract using an arteriosclerosis induction model in rabbits (Yu YM et al, Jpn. J. Pharmacol. 2002, 89, 142-148).

Republic of Korea Patent No. 1815925 Republic of Korea Patent No. 1985779

An object of the present invention is to provide a hydroponic cultivation method of barley sprouts capable of increasing the content of policosanol.

In addition, another object of the present invention is to provide barley sprouts grown according to the hydroponic cultivation method.

In addition, another object of the present invention is to provide a health functional food containing the cultivated barley sprout as an active ingredient.

In addition, another object of the present invention is to provide a cosmetic composition containing the cultivated barley sprout as an active ingredient.

A method for hydroponically cultivating barley sprouts of the present invention for achieving the above object includes the steps of (A) preparing barley sprout seeds on a tray and supplying a nutrient solution so that a part of the barley sprout seeds located on the tray is immersed; (B) spraying bubbles to the barley sprout seeds in contact with the nutrient solution; and (C) irradiating the barley sprout seeds in contact with the nutrient solution with LED light for 1 to 10 hours a day.

The nutrient solution may be purified water.

Between the steps (A) and (B), a step of providing a bioblock so as to be immersed in the nutrient solution contacting the barley sprout seeds while being positioned on the tray; may be added.

The bioblock may emit 120 to 150 ION/cc of negative ions, 0.900 to 0.999 emissivity, and 1.00X10 ² to 9.00X10 ² W/m ² μm of radiant energy.

The bioblock may have a porosity of 30 to 80%.

The bioblock may have a diameter of 5 to 10 cm and a height of 2 to 8 mm.

The bioblock may be added in an amount of 5 to 30 parts by volume based on 100 parts by volume of the nutrient solution.

The bioblock may be added in an amount of 3 to 5 parts by volume based on 100 parts by volume of the nutrient solution.

The bioblock may be basalt calcined at 1700 to 2100 °C.

In step (B), the average particle size of the bubbles may be 30 to 100 μm.

In the step (B), the particle size of the bubbles may be 70% or more of 50 μm or less.

In the step (C), the LED light has a photosynthetic effective light amount of 35 to 50 μmol/m 2 /s, and may be irradiated with a mixed light intensity of blue light and red light.

In addition, barley sprouts with increased policosanol of the present invention for achieving the above-mentioned other objects can be cultivated by the above hydroponic cultivation method.

In addition, the health functional food of the present invention for achieving the above another object may contain the barley sprout as an active ingredient.

In addition, the cosmetic composition of the present invention for achieving the above another object may contain the barley sprout as an active ingredient.

Barley sprouts grown by the hydroponic cultivation method of the present invention can increase the content of policosanol, and are effective in hyperlipidemia, diabetes, blood circulation improvement, hangover relief, and alcoholic fatty liver.

In addition, the hydroponic cultivation method of the present invention can improve the growth of barley sprouts by improving the germination rate and lowering the decay rate, and can provide barley sprouts of excellent quality.

1 is a photograph of a hydroponic cultivation apparatus for irradiating biobubbles and LED light by injecting a nutrient solution into a hydroponic cultivation tray and immersing a bioblock in the nutrient solution according to an embodiment of the present invention.
2 is a view showing bioblocks disposed on a tray according to an embodiment of the present invention.

The present invention relates to a hydroponic cultivation method of barley sprouts capable of increasing the content of policosanol, and to barley sprouts grown according to the method.

The nutrient solution of the present invention is purified water, and when mineral nutrient solution, fermented mineral nutrient solution, etc. is used as the nutrient solution, the content of policosanol does not increase and decay can proceed rapidly.

Hereinafter, the present invention will be described in detail.

The hydroponic cultivation method of barley sprouts in which policosanol is increased according to the present invention includes the steps of (A) preparing barley sprout seeds on a tray and then supplying a nutrient solution so that a part of the barley sprout seeds located on the tray is immersed; (B) spraying microbubbles to the barley sprout seeds in contact with the nutrient solution; and (C) irradiating the barley sprout seeds in contact with the nutrient solution with LED light for 1 to 10 hours.

In addition, between steps (A) and (B) of the present invention, a step of providing a bioblock while being placed on the tray and immersed in a nutrient solution in contact with the barley sprout seeds may be added.

First, in the step (A), barley sprout seeds are prepared on a tray, and then a nutrient solution is supplied so that a part of the barley sprout seeds located on the tray is immersed or brought into contact with each other.

In general, the plant cultivation method using a nutrient solution is a hydroponics method of cultivating plants using a nutrient solution in a liquid state, and can be divided into a spray culture method, a fresh water culture method, a NFT cultivation method, and a drip irrigation method. there is.

The hydroponic cultivation method of plants of the present invention is preferably carried out according to the spray hydroponic cultivation method.

For the nutrient solution, a plurality of sponges equipped with barley sprout seeds are placed on the upper surface of the hydroponic cultivation tray, and the nutrient solution is supplied to the upper surface of the hydroponic cultivation tray so that some of the barley sprout seeds are immersed in or in contact with the nutrient solution. Specifically, since the sponge equipped with barley sprout seeds is disposed in a floating form on the upper surface of the nutrient solution, a part of the barley sprout seeds is immersed in or in contact with the nutrient solution, and the other part of the barley sprout seeds is exposed to the outside. It is preferable that some of the barley sprout seeds are immersed in or in contact with the nutrient solution, but since they only need to be in contact with the nutrient solution, they may be in contact with the nutrient solution through a sponge to absorb moisture from the nutrient solution.

In addition, the bioblock may be provided while being placed on the tray and immersed in the nutrient solution in contact with the barley sprout seeds (FIG. 1).

The bioblock can supply some dissolved oxygen to plants and increase the germination rate.

The bioblock used in the present invention is 120 to 150 ION / cc, preferably 130 to 140 ION / cc, when the number of anions is measured under the conditions of room temperature 24 ℃, humidity 55%, and anion number 116 ION / cc in the air, so dissolved Oxygen can be supplied. In addition, when measured using an FT-IR spectrometer at 37 ° C., an emissivity of 0.900 to 0.999 (5 to 20 μm) and 1.00X10 ² to 9.00X10 ² W / m ² μm are emitted, and 1700 to 1700 It may be basalt calcined at 2100 ° C.

If the negative ion number, emissivity, and radiant energy are out of the above ranges, the content of policosanol may be similar to that of the existing growth environment, and may affect the germination rate of barley sprouts. After germination, it may affect decay during growth. there is.

The bioblock has a porosity of 30 to 80%, preferably 50 to 60%, and when the porosity is less than the lower limit, the number of negative ions, emissivity, and radiant energy are lower than the above range, so that low effects may appear in a desired growth environment. And, if it exceeds the upper limit, the content of policosanol may not increase any more and the concentration of the content may decrease due to overgrowth.

In addition, the bioblock has a diameter of 5 to 10 cm, preferably 7 to 8 cm; The height is 2 to 8 mm, preferably 3 to 5 mm. When the diameter and height of the bioblock are out of the above ranges, the content of policosanol may not increase and the concentration of the content may decrease due to overgrowth.

The bioblock is added in an amount of 5 to 30 parts by volume, preferably 7 to 20 parts by volume, and more preferably 10 to 15 parts by volume, based on 100 parts by volume of the nutrient solution. If the input amount of the bioblock is less than the lower limit, the content of policosanol may not increase, and if the amount exceeds the upper limit, not only does the content of policosanol not increase, but also the growth of barley sprouts decreases and the roots quickly rot.

Specifically, the bioblocks are disposed at intervals of 2 to 9 cm from a position 2 to 4 cm apart from one side of the hydroponic cultivation tray. That is, as shown in FIG. 2, one bioblock is provided at a distance of 2 to 4 cm from one side of the hydroponic cultivation plate, and a plurality of bioblocks are provided at intervals of 2 to 9 cm based on this. When the separation distance of the bioblock is less than the lower limit, the bioblock may not increase the content of policosanol and the germination rate may be low.

Next, in step (B), bubbles are sprayed on barley sprouts that come into contact with the nutrient solution.

The bubbles of the present invention are sprayed toward the upper surface (ie, barley sprouts) of the hydroponic cultivation tray through a bubbler provided on one or more side surfaces of the hydroponic cultivation tray.

The average particle size of the bubbles of the present invention is 30 to 100 μm, preferably 40 to 70 μm, and bubbles with a particle size of 50 μm or less are contained at 70% or more, preferably 70 to 80%. When the average particle size is less than the lower limit, the manufacturing cost increases, and when the average particle size exceeds the upper limit, not only does the content of policosanol not increase, but tissue may be damaged. In addition, when the particle size of 50 μm or less is less than 70%, the germination rate may not be improved and the content of policosanol may not be increased.

Next, in the step (C), LED light is irradiated to the barley sprouts in contact with the nutrient solution for 1 to 10 hours a day, preferably 5 to 10 hours. Specifically, LED light is irradiated to the entire tray equipped with barley sprouts and nutrient solution (FIG. 1).

In the present invention, the amount of photosynthetic effective light is irradiated to be 35 to 50 μmol/m 2 /s.

If the amount of photosynthetic effective light does not satisfy the above range, the content of policosanol cannot be increased and barley sprouts can easily decay. Therefore, it is preferable to use the LED having the effective light amount used in the present invention.

The LED used in the present invention was prepared by mixing red light having a wavelength of 610 to 760 nm and blue light having a wavelength of 435 to 480 nm in order to manufacture an LED satisfying the intensity (Lux) of the present invention. The mixing ratio of the red light and the blue light is not particularly limited as long as it satisfies only the effective light amount within the range of the present invention.

In addition, when the irradiation time of the LED does not satisfy the above range, the content of policosanol may rather decrease and the decay rate may increase.

The present invention can provide a food composition comprising barley sprouts grown according to the hydroponic cultivation method as an active ingredient.

The 'food composition' includes, in addition to plants as active ingredients, food raw materials that can be used as foods described in the food standards and specifications ('food codes') commonly used in food manufacturing, and food additives described in the food additive codes.

It does not need to be particularly limited, but includes, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. The carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sugar, lactose and the like; oligosaccharides or polysaccharides such as dextrin, starch syrup, cyclodextrin and the like; Sugar alcohols such as xylitol, sorbitol, erythritol and the like can be used. As the flavoring agent, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used.

When preparing a food composition using the plant as an active ingredient, the content of the plant does not need to be particularly limited, but is, for example, 0.1 to 99% by weight, 0.5 to 95% by weight, 1 to 90% by weight, 2 to 80% by weight, It may be included in 3 to 70% by weight, 4 to 60% by weight, or 5 to 50% by weight.

Plants, which are active ingredients in the food composition, vary depending on the condition, body weight, presence or absence or degree and duration of disease of the eater, but can be appropriately selected by a person skilled in the art. For example, it may be 1 to 5,000 mg, preferably 5 to 2,000 mg, more preferably 10 to 1,000 mg, still more preferably 20 to 800 mg, and most preferably 50 to 500 mg based on the daily dose. There is, and the number of administrations need not be particularly limited, but can be adjusted by a person skilled in the art within the range of three times a day to once a week. In the case of long-term intake for the purpose of health and hygiene or health control, it may be less than the above range.

The food composition does not need to be particularly limited, but may be, for example, a powder, granule, tablet, capsule, pill, extract, jelly formulation, tea bag formulation or beverage formulation.

In addition, the plant can be added to general food, and the food that can be added does not need to be particularly limited, but for example, confectionery and bread exemplified in the standards and specifications of food according to Article 7 of the Food Sanitation Act ('Food Code') or rice cakes, processed cocoa products or chocolates, processed meat or egg products, processed fish meat products, tofu or jelly, noodles, teas, coffee, beverages, special purpose foods, soybeans, seasonings, dressings, kimchi, salted seafood, pickled foods, It can be added to stewed food, alcoholic beverages, raisins, and other foods. In addition, it can be added to dairy products, processed meat products, packaged meat, and egg products exemplified in the processing standards and ingredient specifications of livestock products ('Livestock Codex') according to Article 4 of the Livestock Products Sanitation Control Act.

On the other hand, the food composition containing the plant as an active ingredient can be used alone as a health functional food.

The 'health functional food' refers to food manufactured (including processing) according to legal standards using raw materials or ingredients having functional properties useful for the human body (Article 3, Subparagraph 1 of the Health Functional Food Act). The term 'health functional food' may differ in terminology or scope from country to country, but 'Dietary Supplement' in the US, 'Food Supplement' in Europe, 'Health Functional Food' or 'Health Functional Food' in Japan. It may correspond to 'Food for Special Health Use (FoSHU)' or 'Health Food' in China.

The food composition or health functional food may additionally contain food additives, and the suitability as a food additive is determined according to the standards and standards for the item in accordance with the general rules and general test methods of the 'Food Additive Code' unless otherwise specified. follow

In addition, the present invention can provide a cosmetic composition comprising barley sprouts in which policosanol is increased as an active ingredient.

In the cosmetic composition of the present invention, in addition to the above cosmetic composition, if necessary, other ingredients commonly used in cosmetics may be mixed. Examples of these ingredients include oils and fats, humectants, emollients, surfactants, organic and inorganic pigments, Organic powders, UV absorbers, preservatives, bactericides, antioxidants, pH adjusters, alcohols, pigments, fragrances, blood circulation promoters, cooling agents, antiperspirants, purified water, water-soluble vitamins, fat-soluble vitamins, high-molecular peptides, polysaccharides, sphingolipids, and seaweed An extract etc. are mentioned.

The cosmetic composition of the present invention may be prepared in the form of an emulsified formulation and a solubilized formulation commonly used in the art.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above ingredients as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural flavors, and A carrier may be further included.

Products to which the composition of the present invention can be added include, for example, mist, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, and sunscreen cream. , Moisture Cream, Hand Cream, Foundation, Essence, Nutritional Essence, Mask Pack, Press Powder, Loose Powder, Eye Shadow, etc. Cosmetics and Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and Body Cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or a propellant such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention, but the following examples are merely illustrative of the present invention, and various changes and modifications are possible within the scope and spirit of the present invention. It is obvious to those skilled in the art, It goes without saying that these variations and modifications fall within the scope of the appended claims.

Preparation Example 1. Bioblock

Basalt calcined at 1800 ° C was used, and the size of the basalt was 8 cmX8 cmX 5 mm (width X length X height), and the porosity was 50%.

The bioblock was commissioned by the Korea Far Infrared Ray Association and tested under the conditions of a room temperature of 24 ° C, humidity of 55%, and anion number of 116 ION / cc in the air with a charged particle measuring device. As a result, the number of negative ions of 136 ION / cc was measured.

As a result of measuring emissivity and radiant energy with an FT-IR spectrometer at 37 °C, emissivity of 0.927 (5 to 20 μm) and radiant energy of 3.57X10 ² W/m ² μm were measured.

Example 1. Bubble + LED

0.825 L of purified water is put into a 3.3 L tray, and a sponge equipped with a plurality of barley sprout seeds is placed on the upper surface of the purified water so that a part of the barley sprout seeds is immersed in the purified water, and bubblers are installed on both sides of the tray While spraying bubbles (average particle size of 30 to 100 μm, 75% of bubbles having a particle size of 50 μm or less) to purified water and barley sprouts, LED light of 35 μmol / m / s of photosynthetic effective light amount per day Irradiated for 5 hours to obtain barley sprouts grown hydroponically.

Example 2. Bubble + LED + Bio Block

0.825 L of purified water is put into a 3.3 L tray, a bioblock is provided to be immersed in the purified water, and a sponge equipped with a plurality of barley sprout seeds is placed on the upper surface of the purified water to immerse a part of the barley sprout seeds in the purified water Bubblers are installed on both sides of the tray to spray bubbles (average particle size of 30 to 100 μm, bubbles with a particle size of 50 μm or less are 75%) to the purified water and barley sprouts, while the photosynthetic effective light amount is 35 μm on the tray ol/m2/s LED light was irradiated for 5 hours a day to obtain hydroponically grown barley sprouts.

Four bioblocks (5 to 30 parts by volume based on 100 parts by volume of purified water) are provided at regular intervals inside the tray.

Comparative Example 1. Use of hydrogenated mineral nutrient solution

In the same manner as in Example 2, barley sprouts were obtained using hydrogenated minerals (with road, KW-923) obtained by mixing and hydrogenating 92 organic and inorganic substances instead of purified water as a nutrient solution.

Comparative Example 2. 150 bioblocks

It was carried out in the same manner as in Example 2, but barley sprouts were obtained using 150 bioblocks.

Comparative Example 3. LED photosynthesis effective light amount 70 μmol / m / s

In the same manner as in Example 2, barley sprouts were obtained by irradiating LED light having a photosynthetically effective light amount of 70 μmol/m2/s instead of 35 μmol/m2/s LED light.

Comparative Example 4. 24-hour LED irradiation

It was carried out in the same manner as in Example 2, but barley sprouts were obtained by irradiating with LED light for 24 hours instead of 5 hours.

The growth conditions in the Examples and Comparative Examples are shown in Table 1 below.

Temperature (℃) Humidity(%) EC (electrical conductivity, ms/cm) pH DO (dissolved oxygen, mg/L) 18 - 22 65 - 75 10-20 6.5-7.5 3-5

<Test Example>

Test Example 1. Measurement of policosanol content

The content of policosanol (1-Hexacosanol) was measured using barley sprouts grown for 10 days according to the above Examples and Comparative Examples.

After injecting 1 g of the freeze-dried sample into a 20 mL vial of Hexane, it was treated with a sonicator at room temperature for 10 minutes. After that, extraction was performed for 24 hours at room temperature using a 200 rpm stirrer. The extraction solution was transferred to a 50 mL conical tube, centrifuged at 7000 rpm and 10 °C for 10 minutes, filtered through a 0.45 μm filter, and concentrated by injecting into a concentrator. The concentrated sample was dissolved in 2 mL CHCl ₃ and then derivatized at 60 °C for 20 minutes by mixing 200 μL of the concentrated solution + 200 μL of MSTFA. After mixing 100 μL + 900 μL CHCl ₃ of the derivative solution, the mixture was filtered through a 0.2 μm filter, put into a GC vial, and analyzed by GC-MS. Instrumental analysis conditions are shown in [Table 2] below.

Barley sprout shoots (Company D, Korea) were used as a control group.

GC-MS HP-5ms, agilent 19091S-431 Detector Mass: Agilent 5977A MSD,
Oven : Agilent 7890B GC system,
Autosampler: Agilent 7693 Equipment Elite Lachrom C18
(2 μm, 2.0 mm Id × 100 mmL) Condition Heater: 250" Mode: Split, 10:1, 1.2 mL/min, He Total flow: 16.2 mL/min Septum purge flow: 3 mL/min oven temperature
: ramp1 - 25 ℃/min_250 ℃ / hold(min): 10
: ramp2 - 20 ℃/min_290 ℃ / hold(min): 7

division control group Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Policosanol (mg/100g) 115.09±2.47 239.05±5.36 298.48±3.92 84.15±0.94 161.37±6.28 94.11±1.06 108.45±1.41

As shown in Table 3 above, it was confirmed that the barley sprouts grown according to Examples 1 and 2 of the present invention had higher policosanol content than those of the control group and Comparative Examples 1 to 4.

In particular, it was confirmed that the barley sprouts of Example 2 had a higher content of policosanol than that of Example 1.

Test Example 2. Measurement of germination rate

The germination rate was obtained by measuring the number of germinated barley sprouts among the barley sprouts cultivated in Examples and Comparative Examples.

Classification (unit: %) day 5 10 Example 1 87.5 95.2 Example 2 90.1 97.8 Comparative Example 1 45.2 40.4 Comparative Example 2 40.5 43.7 Comparative Example 3 50.6 52.8 Comparative Example 4 60.4 57.4

As shown in Table 4 above, it was confirmed that the germination rate of the barley sprouts grown according to Example 1 of the present invention was higher than that of the barley sprouts of Comparative Examples 1 to 4.

Test Example 3. Decay rate measurement

The decay rate was obtained by measuring the number of sprouted barley sprouts among barley sprouts grown in Examples and Comparative Examples.

Classification (unit: %) day 5 10 Example 1 12.5 4.8 Example 2 9.9 2.2 Comparative Example 1 54.8 59.6 Comparative Example 2 59.5 56.3 Comparative Example 3 49.4 47.2 Comparative Example 4 39.6 42.6

As shown in Table 5 above, it was confirmed that the barley sprouts grown according to Example 1 of the present invention had a lower decay rate than the barley sprouts of Comparative Examples 1 to 5.

In Comparative Example 1 and Comparative Example 4, it was confirmed that the decay rate increased over time.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit them, but only to be specifically described.

Formulation Example 1. Preparation of granules

1,000 mg of barley sprout powder obtained in Example 2

Appropriate amount of vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

10 μg of biotin

Nicotinamide 1.7 mg

Folic acid 50 μg

Calcium Pantothenate 0.5 mg

Appropriate amount of mineral mixture

Ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium Carbonate 25.3 mg

Potassium Phosphate Monobasic 15 mg

Dibasic Calcium Phosphate 55 mg

Potassium citrate 90 mg

Calcium Carbonate 100 mg

Magnesium Chloride 24.8 mg

Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for granules in a preferred embodiment, the mixing ratio may be arbitrarily modified, and after mixing the above ingredients according to a conventional granule manufacturing method, It can be prepared and used for preparing a health functional food composition according to a conventional method.

Formulation Example 2. Manufacturing of functional beverages

1,000 mg of barley sprout powder obtained in Example 2

Citric Acid 1,000 mg

100 g of oligosaccharides

2 g plum concentrate

1 g of taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the normal health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and collected in a sterilized 2 L container, sealed and sterilized, and then refrigerated. It is used for preparing the functional beverage composition of the present invention.

Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.

Preparation examples of cosmetic compositions suitable for application of the present invention will be presented.

Formulation Example 3: Lotion

Table 6 shows examples of preparation of lotion among cosmetics containing ginseng sprout powder obtained in Example 1.

ingredient Content (% by weight) Powder of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 4: Lotion

Table 7 shows examples of lotion production among cosmetics containing the ginseng sprout powder obtained in Example 1.

ingredient Content (% by weight) Powder of Example 2 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadamia nut oil 2.0 polysorbate 60 1.5 Sorbitan sesquioleate 1.0 carboxyvinyl polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 5: Nutrition Cream

Table 8 shows examples of preparation of nutritional cream among cosmetics containing the ginseng sprout powder obtained in Example 1.

ingredient Content (% by weight) Powder of Example 2 1.0 Pro-type glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 6: Essence

Table 9 shows examples of the preparation of essence among cosmetics containing the ginseng sprout powder obtained in Example 1.

ingredient Content (% by weight) Powder of Example 2 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 8.0 carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 7: Emulsion for mask pack

Table 10 shows examples of preparation of emulsion for mask pack among cosmetics containing ginseng sprout powder obtained in Example 1.

ingredient Content (% by weight) Powder of Example 2 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Claims (15)

(A) preparing barley sprout seeds on a tray and supplying a nutrient solution so that a part of the barley sprout seeds located on the tray is immersed;
(B) spraying bubbles to the barley sprout seeds in contact with the nutrient solution; and
(C) irradiating the barley sprout seeds in contact with the nutrient solution with LED light for 1 to 10 hours a day. The hydroponic cultivation method of barley sprouts according to claim 1, wherein the nutrient solution is purified water. The method of claim 1, characterized by adding a step of providing a bioblock placed on the tray between steps (A) and (B) while being immersed in a nutrient solution in contact with the barley sprout seeds. A hydroponic cultivation method of barley sprouts in which policosanol is increased. The policosanol according to claim 3, wherein the bioblock emits negative ions of 120 to 150 ION/cc, emissivity of 0.900 to 0.999, and radiant energy of 1.00X10 ² to 9.00X10 ² W/m ² μm. A hydroponic cultivation method of growing barley sprouts. The hydroponic cultivation method of barley sprouts according to claim 3, wherein the bioblock has a porosity of 30 to 80%. The hydroponic cultivation method of barley sprouts according to claim 3, wherein the bioblock has a diameter of 5 to 10 cm and a height of 2 to 8 mm. The hydroponic cultivation method of barley sprouts with increased policosanol according to claim 3, wherein the bioblock is added in an amount of 5 to 30 parts by volume based on 100 parts by volume of the nutrient solution. [Claim 4] The hydroponic cultivation method of barley sprouts with increased policosanol according to claim 3, wherein the bioblock is added in an amount of 7 to 20 parts by volume based on 100 parts by volume of the nutrient solution. The hydroponic cultivation method of barley sprouts with increased policosanol according to claim 3, wherein the bioblock is basalt calcined at 1700 to 2100 °C. The hydroponic cultivation method of barley sprouts with increased policosanol according to claim 1, wherein the average particle size of the bubbles in step (B) is 30 to 100 μm. The hydroponic cultivation method of barley sprouts with increased policosanol according to claim 1, wherein the particle size of the bubbles in step (B) is 50 μm or less and 70% or more. The hydroponic cultivation of barley sprouts with increased policosanol according to claim 1, wherein the LED light in step (C) has a photosynthetic effective light amount of 35 to 50 µmol/m2/s and is a mixture of blue light and red light. method. Barley sprouts grown by the hydroponic cultivation method according to any one of claims 1 to 12 and having increased policosanol. A health functional food containing barley sprouts according to claim 13 as an active ingredient. A cosmetic composition containing the barley sprout of claim 13 as an active ingredient.

Images