KR102623978B1 - Method for hydroponic cultivation of sprout ginseng increasing ginsenoside content and promoting plant growth, and sprout ginseng prepared thereby - Google Patents

Abstract

The present invention relates to a hydroponic cultivation method of ginseng sprouts that increases the ginsenoside content and growth, and to ginseng sprouts produced thereby. (A) After preparing ginseng seeds on a tray, the roots of the ginseng seeds placed on the tray are immersed. Supplying nutrient solution as much as possible; (B) providing the bioblock in a state positioned on a tray and immersed in a nutrient solution in contact with the seed ginseng; and (C) irradiating LED light to the seed ginseng in contact with the nutrient solution in which the bioblock is immersed. By including this, the content of ginsenosides can be increased and growth can be improved.

Description

Hydroponic cultivation method of sprout ginseng to increase ginsenoside content and growth and sprout ginseng prepared thereby {Method for hydroponic cultivation of sprout ginseng increasing ginsenoside content and promoting plant growth, and sprout ginseng prepared thereby}

The present invention relates to a hydroponic cultivation method of ginseng sprouts that can increase the content of ginsenosides and improve growth, and to ginseng sprouts produced thereby.

Hydroponic cultivation is a method of growing plants using water and water-soluble nutrients using artificial media or containers that can support plants without using soil. It is called water cultivation, nutrient solution cultivation, and soilless cultivation. This type of hydroponic cultivation has the advantage of precise supply and management of fertilizer ingredients and no concerns about soil disruption to continuous cultivation, so its area is gradually increasing.

Additionally, the advantages of hydroponics include the ability to directly observe the condition and growth of roots, the ability to continuously produce clean, uncontaminated vegetables and plants, and commercial mass production. The multi-purpose indoor hydroponic cultivation device can be easily grown at home by anyone, so it can be used as daily gardening and also plays a role in cultivating emotions.

Plants that can be grown hydroponically include dicotyledonous plants or mostly monocotyledonous plants with many fibrous roots, fruit and vegetables such as cucumbers, tomatoes, peppers, paprika and strawberries, leafy vegetables such as lettuce, lettuce, kale and water parsley, chrysanthemums, roses, gerberas, lilies, Flowers such as begonia and anthurium, ginseng, and even some medicinal plants can all be grown hydroponically.

Recently, various technologies have been developed for hydroponic cultivation of crops whose roots are used as food, such as bellflower root and deodeok, including ginseng with improved saponin content. In addition, as interest in the pharmacological effects of root hairs in root plants increases, many academic studies are being conducted.

The conventional method of acquiring ginseng seedlings, i.e., growing ginseng, has limitations in that it relies on pesticides, and even then, the death rate of seedlings is high depending on the amount of irrigation and time, and the production rate of quality seedlings that can be transplanted is low, so the supply and demand of ginseng seedlings must be planned. It cannot be implemented as such, and there are problems such as difficulty in supplying seedlings in large quantities.

Recently, as seen in Patent Registration No. 1780480, Patent Registration No. 1802874, and Patent Registration No. 1902360, various types of hydroponic cultivation systems have been developed to produce young ginseng seedlings through hydroponic cultivation.

Currently, hydroponic cultivation uses a method of transplanting 1-2 year old ginseng or ginseng seedlings and harvesting them after growing for about 3 months. The hydroponic cultivation method does not require management of planned land and allows for continuous cultivation, but requires a lot of initial capital and requires cultivation technology. (Solution management, etc.) Also, because it is complicated, there is a disadvantage in that it is difficult to cultivate ginseng using hydroponic methods without the help of experts.

Therefore, there is a need for a hydroponic cultivation method for ginseng sprouts that is easy to cultivate, increases the content of ginsenosides, and improves growth.

Republic of Korea Patent Registration No. 1780480 Republic of Korea Patent Registration No. 1802874 Republic of Korea Patent Registration No. 1902360

The purpose of the present invention is to provide a hydroponic cultivation method of ginseng sprouts that can increase the content of ginsenosides and improve growth.

In addition, another object of the present invention is to provide ginseng sprouts produced according to the above cultivation method.

In addition, another object of the present invention is to provide a health functional food containing the above-mentioned ginseng sprout as an active ingredient.

In addition, another object of the present invention is to provide a cosmetic composition containing the ginseng sprout as an active ingredient.

The method for hydroponically cultivating ginseng sprouts of the present invention to achieve the above object includes the steps of (A) preparing ginseng seeds on a tray and then supplying a mineral nutrient solution so that the roots of the ginseng seeds located on the tray are immersed; (B) providing bioblocks to be immersed in a mineral nutrient solution located on the tray and in contact with the seed ginseng; and (C) irradiating LED light to the seed ginseng in contact with the mineral nutrient solution in which the bioblock is immersed.

In the hydroponic cultivation method, 80 to 100 L/min of oxygen bubbles can be supplied to the nutrient solution based on 960 L of the mineral nutrient solution.

In step (A), the seed ginseng may be 1 to 2 years old.

In step (A), the mineral nutrient solution may be a mixture of 0.05 to 0.5 parts by weight of mineral solution with respect to 100 parts by weight of purified water.

The mineral solution contains 4900 to 5200 mg/L of calcium (Ca), 480 to 520 mg/L of magnesium (Mg), 70 to 80 mg/L of potassium (K), and 90 to 120 mg/L of strontium (Sr). ), 0.02 to 0.05 mg/L of iron (Fe), 0.03 to 0.08 mg/L of manganese (Mn), and 2200 to 2400 mg/L of sodium (Na).

In step (B), the bioblock may emit negative ions of 120 to 150 ION/cc, an emissivity of 0.900 to 0.999, and radiant energy of 1.00X10 ² to 9.00X10 ² W/m ² ·㎛. The emissivity refers to the energy radiated from the bioblock.

In step (B), the bioblock may have a porosity of 30 to 80%.

In step (B), the diameter of the bioblock may be 5 to 10 cm, and the height may be 2 to 8 mm.

In step (B), bioblocks may be added in an amount of 1 to 10 parts by volume per 100 parts by volume of the mineral nutrient solution.

In step (B), the bioblock may be basalt calcined at 1700 to 2100 °C.

In step (C), the LED light may be irradiated at an intensity of 18,000 to 30,000 Lux for 24 hours a day.

In addition, ginseng sprouts of the present invention to achieve the above-mentioned other purposes can be produced according to the above production method.

In addition, the health functional food of the present invention to achieve the above-described other purpose may contain ginseng sprouts as an active ingredient.

In addition, the cosmetic composition of the present invention for achieving the above-described other object may contain ginseng sprouts as an active ingredient.

Ginseng sprouts grown hydroponically according to the present invention can increase the content of ginsenosides and promote growth.

In particular, the content of Re, Rg2, and Rd in ginseng sprouts hydroponically grown according to the present invention was significantly increased compared to ginseng seeds and ginseng sprouts grown in soil, and the content of F1, which was not detected in ginseng seeds and ginseng sprouts grown in soil, was high. It was detected.

Figure 1 is a photograph of ginseng seeds penetrated into a sponge according to an embodiment of the present invention.
Figure 2 is a photograph of a hydroponic cultivation device in which nutrient solution is added to a hydroponic cultivation tray, bioblocks are immersed in the nutrient solution, and LED light is irradiated according to an embodiment of the present invention.
Figure 3 is a diagram showing bioblocks placed on a tray according to an embodiment of the present invention.

The present invention relates to a hydroponic cultivation method of ginseng sprouts that can increase the content of ginsenosides and improve growth, and ginseng sprouts produced thereby.

Hereinafter, the present invention will be described in detail.

The method of hydroponically cultivating ginseng sprouts to increase ginsenoside content and growth of the present invention includes the steps of (A) preparing ginseng seeds on a tray and then supplying a mineral nutrient solution so that the roots of the ginseng seeds placed on the tray are immersed. ; (B) providing bioblocks immersed in a mineral nutrient solution located on the tray and in contact with the seed ginseng; and (C) irradiating LED light to the seed ginseng in contact with the mineral nutrient solution in which the bioblock is immersed. In addition, during hydroponic cultivation, oxygen bubbles at 80 to 100 L/min, preferably 85 to 95 L/min, can be supplied to the mineral nutrient solution based on 960 L of the mineral nutrient solution. If oxygen bubbles are not supplied to the mineral nutrient solution, the content and growth of ginsenosides may be low.

First, in step (A), ginseng seeds are prepared on a tray, and then a mineral nutrient solution is supplied so that the roots of the ginseng seeds placed on the tray are immersed.

Seed ginseng is 1 to 2 year old ginseng seedlings grown for transplantation before being planted. In the present invention, it is preferable to use 1 year old ginseng seeds for better ginsenoside content and growth.

In general, the plant cultivation method using nutrient solution is a hydroponic method of cultivating plants using liquid nutrient solution, and can be divided into spray culture method, submersion culture method, NFT cultivation method, and drip irrigation method. there is.

Since the hydroponic cultivation method of ginseng sprouts of the present invention uses bioblocks, it is preferably carried out according to the freshwater cultivation method.

The mineral nutrient solution is prepared by placing a plurality of sponges with seed ginseng (the root part of the seed ginseng penetrates the sponge, Figure 1) on the upper surface of the hydroponic cultivation tray, and supplying the mineral nutrient solution to the upper surface of the hydroponic cultivation tray. The root of the ginseng seed is immersed in the water. Specifically, the sponge with seed ginseng is placed floating on the upper surface of the mineral nutrient solution, so the root part of the ginseng seed that penetrates the sponge is immersed in the mineral nutrient solution, and other parts of the seed ginseng are exposed to the outside.

The mineral nutrient solution used in the present invention is a mixture of 0.05 to 0.5 parts by weight of mineral solution, preferably 0.1 to 0.4 parts by weight, and more preferably 0.1 to 0.2 parts by weight, based on 100 parts by weight of purified water. If the content of the mineral solution is less than the lower limit, the desired effect cannot be obtained, and if it exceeds the upper limit, growth and ginsenoside content may not be improved and decay may progress quickly.

The mineral solution contains 4900 to 5200 mg/L of calcium (Ca), 480 to 520 mg/L of magnesium (Mg), 70 to 80 mg/L of potassium (K), and 90 to 120 mg/L of strontium (Sr). ), 0.02 to 0.05 mg/L of iron (Fe), 0.03 to 0.08 mg/L of manganese (Mn), and 2200 to 2400 mg/L of sodium (Na).

Next, in step (B), the bioblock is placed on the tray and immersed in a mineral nutrient solution in contact with the seed ginseng (FIG. 2).

The bioblock can supply some dissolved oxygen to ginseng seeds, and when used with the nutrient solution, it can improve the growth of ginseng sprouts and increase the ginsenoside content.

The bioblock used in the present invention is dissolved because the negative ion water is measured at 120 to 150 ION/cc, preferably 130 to 140 ION/cc, under the conditions of room temperature of 24°C, humidity of 55%, and negative ion water in the air of 116 ION/cc. Supply of oxygen is possible. In addition, when measured using ^a FT-IR spectrometer at ³⁷ °C, an emissivity of 0.900 to 0.999 (5 to 20 ㎛) and radiant energy of ^1.00 It may be basalt calcined at 2100°C.

If the anion water, emissivity, and radiant energy are outside the above ranges, growth and ginsenoside content may not be improved even if used with a mineral nutrient solution.

The bioblock has a porosity of 30 to 80%, preferably 40 to 60%. If the porosity is less than the lower limit, the anion water, emissivity and radiant energy are lower than the above range, so the desired effect cannot be obtained, and the upper limit is In case of excess, it can break easily and the roots can easily rot.

Additionally, the bioblock has a diameter of 5 to 10 cm, preferably 7 to 8 cm; The height is 2 to 8 mm, preferably 3 to 5 mm. If the diameter and height of the bioblock are outside the above range, the growth of ginseng sprouts may be hindered.

The bioblock is added in an amount of 1 to 10 parts by volume, preferably 2 to 4 parts by volume, per 100 parts by volume of the mineral nutrient solution. If the amount of bioblock input is less than the above lower limit, growth and ginsenoside content may not be improved, and if it exceeds the above upper limit, the growth of ginseng sprouts is reduced and the roots rot quickly.

Specifically, bioblocks are arranged at intervals of 2 to 9 cm from a position 2 to 4 cm away from one side of the hydroponic cultivation tray. That is, as shown in Figure 3, one bioblock is provided at a distance of 2 to 4 cm from one side of the hydroponic cultivation plate, and a plurality of bioblocks are provided at intervals of 2 to 9 cm based on this. If the separation distance between bioblocks is less than the lower limit, the roots of ginseng sprouts rot quickly, and if it exceeds the upper limit, growth and ginsenoside content may increase to a small extent.

Next, in step (C), the seed ginseng in contact with the mineral nutrient solution in which the bioblock is immersed is irradiated with LED light for 20 to 24 hours a day, preferably 22 to 24 hours. Specifically, LED light is radiated to the entire tray equipped with seed ginseng, mineral nutrient solution, and bioblocks (Figure 2).

Generally, the intensity of LED light is 65,535 Lux, but in the present invention, it is irradiated at an intensity lower than the above intensity of 18,000 to 30,000 Lux, preferably 20,000 to 250,000 Lux.

When using a general LED with a light intensity of 65,535 Lux, growth may not proceed or may progress slowly, and the ginsenoside content may not increase, so it is preferable to use an LED with the intensity used in the present invention. do.

The LED used in the present invention was manufactured by mixing red light with a wavelength of 610 to 760 nm, blue light with a wavelength of 435 to 480 nm, and white light to produce an LED that satisfies the intensity (Lux) of the present invention. The mixing ratio of the red light, blue light, and white light is not particularly limited and only needs to satisfy the intensity (Lux) within the range of the present invention.

Additionally, if the LED irradiation time is less than the above lower limit, growth progresses slowly and the content of ginsenoside may not increase.

The present invention can provide a food composition containing ginseng sprouts grown according to the above hydroponic cultivation method as an active ingredient.

The 'food composition' includes, in addition to ginseng sprouts as an active ingredient, food raw materials that can be used as foods as described in the standards and specifications for foods commonly used in food production ('food code'), and food additives listed in the food additive code. .

There is no need to specifically limit it, but examples include proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. The carbohydrates include monosaccharides, such as glucose, fructose, etc.; Disaccharides such as maltose, sugar, lactose, etc.; Oligosaccharides or polysaccharides such as dextrin, starch syrup, cyclodextrin, etc.; Sugar alcohols such as xylitol, sorbitol, erythritol, etc. can be used. The flavoring agent may be a natural flavoring agent (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)) or a synthetic flavoring agent (saccharin, aspartame, etc.).

When manufacturing a food composition using the above-mentioned ginseng sprout as an active ingredient, the content of ginseng sprout does not need to be specifically limited, but is, for example, 0.1 to 99% by weight, 0.5 to 95% by weight, 1 to 90% by weight, 2 to 80% by weight. %, 3 to 70% by weight, 4 to 60% by weight, and 5 to 50% by weight.

Ginseng sprouts, which are the active ingredient in the food composition, may vary depending on the condition, weight, presence, degree, and period of the disease of the consumer, but may be appropriately selected by a person skilled in the art. For example, based on the daily dosage, it may be 1 to 5,000 mg, preferably 5 to 2,000 mg, more preferably 10 to 1,000 mg, even more preferably 20 to 800 mg, and most preferably 50 to 500 mg. There is no need to specifically limit the number of administrations, but a person skilled in the art can adjust it within the range of three times a day to once a week. In the case of long-term intake for health and hygiene purposes or health control, it may be below the above range.

The food composition does not need to be particularly limited, but may be, for example, powder, granule, tablet, capsule, pill, extract, jelly formulation, tea bag formulation, or beverage formulation.

In addition, in order to provide the functionality of ginsenosides to general foods, the above-mentioned ginseng sprouts can be added. There is no need to specifically limit the foods that can be added, but for example, the food standards and specifications pursuant to Article 7 of the Food Sanitation Act (' Confectionery, bread or rice cake, processed cocoa products or chocolate, processed meat or egg products, processed fish products, tofu or jelly, noodles, tea, coffee, beverages, special purpose foods, sauces, seasonings, dressings as exemplified in the ‘Food Code of Conduct’) It can be added to seafood, kimchi, salted seafood, pickled foods, stewed foods, alcoholic beverages, dried raisins, and other foods. In addition, it can be added to dairy products, processed meat products, packaged meat, and egg products as exemplified in the livestock product processing standards and ingredient specifications ('Livestock Product Code') pursuant to Article 4 of the Livestock Products Sanitation Management Act.

Meanwhile, the food composition containing ginseng sprouts as an active ingredient can be used alone as a health functional food.

The above ‘health functional food’ refers to food manufactured (including processing) in accordance with legal standards using raw materials or ingredients with functional properties useful to the human body (Article 3, Paragraph 1 of the Health Functional Food Act). The terminology or scope of the above 'health functional food' may vary depending on the country, but it is also called 'Dietary Supplement' in the United States, 'Food Supplement' in Europe, 'Health Functional Food' or 'Health Functional Food' in Japan. It may be classified as ‘Food for Special Health Use (FoSHU)’ or ‘Health Food’ in China.

The above food composition or health functional food may additionally contain food additives, and its suitability as a food additive is determined by the specifications and standards for the relevant item in accordance with the general provisions of the ‘Food Additive Code’ and general test methods, etc., unless otherwise specified. Follow.

Additionally, the present invention can provide a cosmetic composition containing ginseng sprouts as an active ingredient.

In addition to the above cosmetic composition, the cosmetic composition of the present invention can be blended with other ingredients that are usually blended in cosmetics as needed. These ingredients include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, Organic powder, ultraviolet absorber, preservative, disinfectant, antioxidant, pH adjuster, alcohol, colorant, fragrance, blood circulation promoter, coolant, antiperspirant, purified water, water-soluble vitamin, fat-soluble vitamin, polymer peptide, polymer polysaccharide, sphingolipid and seaweed. Extracts, etc. can be mentioned.

The cosmetic composition of the present invention can be prepared in the form of emulsified formulations and solubilized formulations commonly used in the art.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the ingredients as active ingredients, for example, conventional auxiliaries such as stabilizers, pigments, and natural fragrances, and It may further contain a carrier.

Products to which the composition of the present invention can be added include, for example, mist, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, and UV protection cream. , cosmetics such as moisture cream, hand cream, foundation, essence, nutritional essence, mask pack, press powder, loose powder, eye shadow, etc., as well as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. , butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention. However, the following examples are merely illustrative of the present invention, and it is clear to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention. It is natural that such variations and modifications fall within the scope of the attached patent claims.

Preparation Example 1. Bioblock

Basalt fired at 1800°C was used, and the size of the basalt was 8 cm

The above bioblock was commissioned by the Korea Far-Infrared Association and tested using a charged particle measuring device under the conditions of room temperature of 24°C, humidity of 55%, and negative ion water in the air of 116 ION/cc. As a result, the negative ion water of 136 ION/cc was measured.

As a result of measuring the emissivity and radiant energy with a FT-IR spectrometer at 37 ℃, an emissivity of 0.927 (5~20 ㎛) and a radiant energy of 3.57X10 ² W/m ² ·㎛ were measured.

Control group 1.

Two-year-old ginseng seeds were used.

Control group 2.

Ginseng sprouts were obtained by cultivating 2-year-old ginseng seeds in soil.

Example 1. 2-year-old ginseng seeds

Inject 960 L of mineral nutrient solution into a 1200 L tray, provide bioblocks to be immersed in the mineral nutrient solution, and then place a sponge with a plurality of 2-year-old ginseng seeds pierced on the upper surface of the mineral nutrient solution to cover the roots of the seed ginseng. It was immersed in this mineral nutrient solution, and 21,626 Lux of LED light was irradiated on the tray for 24 hours a day to obtain hydroponically grown ginseng sprouts.

The mineral nutrient solution is a mixture of 0.1 parts by weight of mineral solution with respect to 100 parts by weight of purified water, and the mineral solution contains 5050 mg/L of calcium (Ca), 509 mg/L of magnesium (Mg), and 75.1 mg/L of potassium. (K), containing 109 mg/L strontium (Sr), 0.04 mg/L iron (Fe), 0.05 mg/L manganese (Mn), and 2330 mg/L sodium (Na).

In addition, oxygen bubbles of 80 to 100 L/min are supplied to the mineral nutrient solution through an oxygen generator based on 960 L of the mineral nutrient solution, and 40 bioblocks are placed inside the tray (2.3 parts per 100 parts by volume of the mineral nutrient solution). skin) is provided at regular intervals.

Example 2. One-year-old ginseng seeds

The same procedure as Example 1 was performed, except that 1-year-old ginseng seeds were used instead of 2-year-old ginseng seeds to obtain hydroponically grown ginseng sprouts.

Comparative Example 1. Use of purified water as nutrient solution

The same procedure as in Example 1 was performed, but hydroponic ginseng sprouts were obtained using purified water as the nutrient solution.

Comparative Example 2. Bioblock omitted

The same procedure as Example 1 was performed, but hydroponic ginseng sprouts were obtained without using bioblocks.

Comparative Example 3. 10 bioblocks

The same procedure as in Example 1 was performed, except that 10 bioblocks were used to obtain ginseng sprouts.

Comparative Example 4. 100 bioblocks

The same procedure as Example 1 was performed, except that 100 bioblocks were used to obtain ginseng sprouts.

Comparative Example 5. LED 65,535 Lux

The same procedure as in Example 1 was performed, except that 65,535 Lux of regular LED light was used instead of 21,626 Lux of LED light to obtain ginseng sprouts.

Comparative Example 6. LED irradiation for 12 hours

The same procedure as in Example 1 was performed, but ginseng sprouts were obtained by irradiating LED light for 12 hours instead of 24 hours.

The growth conditions in the examples and comparative examples are shown in [Table 1] below.

Temperature (℃) humidity(%) EC (electrical conductivity, ms/cm) pH DO (dissolved oxygen, mg/L) 23-26 65.31 10-20 6.5-7.5 3-5

<Test example>

Test example 1. Measurement of root length, plant length, root diameter, and number of leaves

The total length, root length, plant length, root diameter, and number of leaves of ginseng sprouts grown in Examples and Comparative Examples were measured. Root length refers to the length of the root, and plant length refers to the length of the stem.

division day 0 7 14 Control group 2 Total length (mm) - 181.6 208.7 Muscle length (mm) 124.9 127.4 129.9 Total length (mm) - 54.2 78.8 Root diameter (mm) 41.9 41.5 42.2 Number of leaves (pcs) - 9.8 10.6 Example 1 Total length (mm) - 188.1 225.2 Muscle length (mm) 125.5 129.8 131.9 Total length (mm) - 58.3 93.3 Root diameter (mm) 42.1 46.0 38.9 Number of leaves (pcs) - 10.2 11.6 Example 2 Total length (mm) - 209.1 234.7 Muscle length (mm) 125.7 130.6 133.4 Total length (mm) - 78.5 101.3 Root diameter (mm) 42.3 47.2 43.3 Number of leaves (pcs) - 12.1 13.5 Comparative Example 1 Total length (mm) - 183.8 209.1 Muscle length (mm) 125.0 128.9 130.3 Total length (mm) - 54.9 78.8 Root diameter (mm) 41.8 42.7 42.7 Number of leaves (pcs) - 10.4 10.5 Comparative Example 2 Total length (mm) - 170.4 201.5 Muscle length (mm) 125.6 126.2 129.0 Total length (mm) - 44.4 72.5 Root diameter (mm) 42.5 42.5 42.9 Number of leaves (pcs) - 10.0 10.5 Comparative Example 3 Total length (mm) - 187.4 213.5 Muscle length (mm) 124.2 129.1 131.0 Total length (mm) - 58.3 82.5 Root diameter (mm) 41.4 44.1 44.4 Number of leaves (pcs) - 10.2 10.7 Comparative Example 4 Total length (mm) - 150.1 182.5 Muscle length (mm) 125.6 114.1 120.0 Total length (mm) - 36.0 62.5 Root diameter (mm) 42.4 42.3 42.2 Number of leaves (pcs) - 5.11 5.56 Comparative Example 5 Total length (mm) - 158.2 170.7 Muscle length (mm) 125.1 116.2 118.4 Total length (mm) - 42.0 52.3 Root diameter (mm) 42.0 42.3 42.6 Number of leaves (pcs) - 6.0 4.8 Comparative Example 6 Total length (mm) - 166.4 197.9 Muscle length (mm) 125.0 126.6 128.2 Total length (mm) - 39.8 69.7 Root diameter (mm) 42.9 38.6 41.4 Number of leaves (pcs) - 9.53 9.20

As shown in Table 2 above, it was confirmed that the ginseng sprouts cultivated according to Examples 1 and 2 of the present invention were superior to Comparative Examples 1 to 6 in terms of total length, root length, plant length, root diameter, and number of leaves.

Test Example 2. Ginsenoside measurement

The contents of Rg1, Re, Rg2, Gb1, F1, and Rd contained in ginseng sprouts grown in Examples and Comparative Examples were measured.

The measurement method was to store the sample in its original form in a freezer at -80°C and use it in the experiment. To analyze ginsenosides, the sample was pulverized and homogenized, and 0.5 g of the sample was placed in a 22 mL amber vial lined with boiling stones, 10 mL of 50% MeOH was added, and heat extraction was performed at 80°C for 10 minutes in a heating block. After cooling, the supernatant was transferred to a 25 mL volumetric flask, and the residue was repeated once more and then dissolved in 50% MeOH in a 25 mL volumetric flask. It was used in the experiment after filtration with a membrane filter (0.20 μm), and was analyzed using a liquid chromatograph/ultraviolet absorbance detector (Hitachi Elite Lachrom L-2000 Series) and quantitative analysis was performed at the maximum absorption wavelength of 203 nm. .

HPLC Hitachi Elite Lachrom L-2000 Series Detector L-2400U Ultra Violet Detector Column Elite Lachrom C18
(2 μm, 2.0 mm Id × 100 mmL) Mobile phase A 20% ACN Mobile phase B 80% ACN Flow rate 0.3mL Column oven temp. 30℃ Wavelength 203nm injection vol. 5 μL

Category (mg/100g) Rg1 Re Rg2 Rb1 F1 Rd total Control group 1 43.22 69.59 3.46 37.87 0.00 4.05 158.19 Control group 2 36.52 95.23 5.31 22.57 0.00 3.50 162.13 Example 1 73.10 347.64 17.68 42.91 19.75 52.54 553.62 Example 2 96.72 427.66 34.33 27.25 45.23 106.56 737.75 Comparative Example 1 45.18 170.11 10.62 30.15 4.56 50.01 310.63 Comparative Example 2 38.18 111.15 8.34 23.18 2.07 39.65 456.84 Comparative Example 3 55.16 253.18 19.11 30.45 13.44 48.15 419.49 Comparative Example 4 50.17 98.99 4.82 20.68 1.18 20.69 196.53 Comparative Example 5 51.44 164.67 7.94 27.44 5.12 45.67 302.28 Comparative Example 6 52.60 262.22 18.08 28.99 12.75 48.82 423.46

As shown in Table 4 above, it was confirmed that the ginseng sprouts grown according to Examples 1 and 2 of the present invention had a higher content of ginsside compared to Comparative Examples 1 to 6. In particular, F1 was not detected at all in Control Group 1 and Control Group 2, but was detected in Examples 1 and 2 and Comparative Examples 1 to 6, and it was confirmed that Example 2 had the best content among each group.

Furthermore, it was confirmed that among Examples 1 and 2, Comparative Examples 1 to 6, Control Group 1 and Control Group 2, Example 2 had the highest content of all ginsenosides.

Below, a formulation example of a composition containing the powder of the present invention is described, but the present invention is not intended to be limited, but merely explained in detail.

Formulation Example 1. Preparation of granules

1,000 mg of ginseng sprout powder obtained in Example 1

Vitamin mixture dosage

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 ㎍

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

Folic acid 50 μg

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

Ferrous sulfate 1.75 mg

Zinc oxide 0.82 mg

Magnesium carbonate 25.3 mg

Monobasic Potassium Phosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

The composition ratio of the above vitamin and mineral mixture is a mixture of components relatively suitable for granules in a preferred embodiment, but the mixing ratio may be modified arbitrarily. The above components are mixed according to a typical granule manufacturing method, and then the granules are mixed. It can be prepared and used to manufacture a health functional food composition according to a conventional method.

Formulation Example 2. Preparation of functional beverage

1,000 mg of ginseng sprout powder obtained in Example 1

1,000 mg citric acid

100 g oligosaccharides

2 g plum concentrate

1 g taurine

Add purified water to make a total of 900 mL.

After mixing the above ingredients according to a typical health drink manufacturing method, stirring and heating at 85°C for about 1 hour, the resulting solution was filtered, placed in a sterilized 2 L container, sealed, sterilized, stored in the refrigerator, and then refrigerated. Used for manufacturing the functional beverage composition of the invention.

The composition ratio is a preferred embodiment of mixing ingredients relatively suitable for beverages of preference, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as demand class, country of demand, and intended use.

An example of manufacturing a cosmetic composition suitable for applying the present invention will be presented.

Formulation Example 3: Toner

The manufacturing example of lotion among cosmetics containing ginseng sprout powder obtained in Example 1 is shown in Table 5 below.

ingredient Content (% by weight) Powder of Example 1 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 4: Lotion

Preparation examples of lotions among cosmetics containing the ginseng sprout powder obtained in Example 1 are shown in Table 6 below.

ingredient Content (% by weight) Powder of Example 1 3.0 propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadami nut oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxy vinyl polymer 1.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 5: Nutritional Cream

The manufacturing example of nutritional cream among cosmetics containing ginseng sprout powder obtained in Example 1 is shown in Table 7 below.

ingredient Content (% by weight) Powder of Example 1 1.0 Lipophilic glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 6: Essence

Preparation examples of essences in cosmetics containing ginseng sprout powder obtained in Example 1 are shown in Table 8 below.

ingredient Content (% by weight) Powder of Example 1 1.5 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethylcellulose 0.1 Sodium Hyaluronate 8.0 Carboxy vinyl polymer 0.2 Triethanolamine 0.18 octyldodecanol 0.3 Octyldodeceth-16 0.4 ethanol 6.0 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Formulation Example 7: Emulsion for mask pack

The manufacturing example of the emulsion for a mask pack among cosmetics containing the ginseng sprout powder obtained in Example 1 is shown in Table 9 below.

ingredient Content (% by weight) Powder of Example 1 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 Hydrogenated Castor Oil 0.3 Preservatives, fragrance, coloring a very small amount Distilled water remaining amount Sum 100

Claims (14)

(A) preparing ginseng seeds on a tray and then supplying a mineral nutrient solution so that the roots of the ginseng seeds placed on the tray are immersed;
(B) providing bioblocks immersed in a mineral nutrient solution located on the tray and in contact with the seed ginseng; and
(C) irradiating LED light to the seed ginseng in contact with the mineral nutrient solution in which the bioblock is immersed;
In step (A), the mineral nutrient solution is mixed with 0.05 to 0.5 parts by weight of mineral solution based on 100 parts by weight of purified water, and the mineral solution contains 4900 to 5200 mg/L of calcium (Ca) and 480 to 520 mg/L of magnesium ( Mg), 70 to 80 mg/L potassium (K), 90 to 120 mg/L strontium (Sr), 0.02 to 0.05 mg/L iron (Fe), 0.03 to 0.08 mg/L manganese (Mn). and 2200 to 2400 mg/L of sodium (Na),
In step (B), the bioblocks have a diameter of 5 to 10 cm and a height of 2 to 8 mm, so the bioblocks are spaced apart, so they are provided in an amount of 1 to 10 parts by volume per 100 parts by volume of the mineral nutrient solution,
In step (C), LED light is irradiated for 20 to 24 hours a day at an intensity of 18,000 to 30,000 Lux. A hydroponic cultivation method of ginseng sprouts that increases ginsenoside content and growth. The hydroponic cultivation method of ginseng sprouts according to claim 1, wherein in the hydroponic cultivation method, oxygen bubbles are supplied to the nutrient solution at a rate of 80 to 100 L/min based on 960 L of the mineral nutrient solution. The hydroponic cultivation method of ginseng sprouts according to claim 1, wherein in step (A), the ginseng seeds are 1 to 2 years old. delete delete The method of claim 1, wherein in step (B), the bioblock emits negative ions of 120 to 150 ION/cc, an emissivity of 0.900 to 0.999, and radiant energy of 1.00X10 ² to 9.00X10 ² W/m ² ·㎛. Characterized by hydroponic cultivation method of sprouts. The hydroponic cultivation method of ginseng sprouts according to claim 1, wherein in step (B), the bioblock has a porosity of 30 to 80%. delete delete The method of hydroponic cultivation of ginseng sprouts according to claim 1, wherein the bioblock in step (B) is basalt calcined at 1700 to 2100°C. delete Ginseng sprouts grown using the hydroponic cultivation method according to any one of paragraphs 1, 2, 3, 6, 7, and 10. A health functional food containing the ginseng sprout of Paragraph 12 as an active ingredient. A cosmetic composition containing the ginseng sprout of claim 12 as an active ingredient.