KR20190020924A - Method for cultivating ginseng and ginseng cultivated by the same - Google Patents

Abstract

The present invention relates to a method for cultivating Panax ginseng and Panax ginseng cultivated by the method. The method for cultivating Panax ginseng according to an embodiment of the present invention comprises: making Panax ginseng seedlings sprout in a box at a temperature of 15 to 20°C and then transplanting the seedlings into an aquaculture facility; adjusting condition to a temperature range of 15 to 20°C and a humidity range of 80 to 90% in a seedling stage of Panax ginseng; adjusting condition to a temperature range of 21 to 25°C and a humidity range of 65 to 75% in a vegetative growth period of Panax ginseng; and supplying methyl jasmonate to Panax ginseng. A content of saponin can increase in Panax ginseng cultivated according to the present invention.

Description

[0001] The present invention relates to a ginseng cultivation method and a method for cultivating ginseng cultivated by the method,

The present invention relates to a ginseng cultivation method and a ginseng cultivated by the method, more specifically, to a ginseng cultivation method capable of increasing the content of saponin and a ginseng cultivated by the method.

Ginseng is a perennial herbaceous herbaceous plant belonging to the genus Panax of Araliaceae and has long been used as an important medicinal herb in plant medicine.

Ginseng has been exhibiting remarkable efficacy in treating various diseases and promoting the recovery of diseases and has been extensively studied to investigate the effects of ginseng on the efficacy and pharmacological effects of ginseng. Based on these actions, antithezoid and anti-stress action, anti-diabetic effect, blood pressure control action, anti-cancer action, prevention of arteriosclerosis and hypertension, brain Enhancement of function, enhancement of gastrointestinal function, enhancement of immune function, and antiviral action.

The study of ginseng ingredients began in the United States with the first scientific study since the amorphous glycoside mixture was separated and named Panaquilon.

It has been scientifically proven that ginseng has various pharmacological effects. Such action is known to be mainly caused by the ginseng glycoside (saponin) component, and other active ingredients include saponin, polyacetylene, pyrazine derivatives, Maltol and the like are known.

Natural medicinal ginseng, however, is a non-economic product that is well-drained and cultivated over a long period of 4-6 years in cool highlands, affected by natural climates, and impossible to serialize in the same place. Therefore, various studies have been conducted to more easily grow expensive medicinal ginseng.

Korean Patent Publication No. 1993-0000004 Korean Patent No. 10-1163555

An object of one embodiment of the present invention is to provide a ginseng cultivation method capable of increasing the content of saponin and ginseng cultivated by the method.

One embodiment of the present invention includes a step of seedling the seedlings in a box at a temperature of 15 to 20 占 폚 and then filling the seedlings into a hydroponic cultivation facility; Adjusting the temperature of the ginseng seedling to a temperature range of 15 to 20 ° C and a humidity range of 80 to 90%; Adjusting the temperature of the ginseng to a temperature range of 21 to 25 ° C and a humidity range of 65 to 75%; And feeding ginseng with methyl jasmonate. The present invention also provides a method for growing ginseng.

The methyl jasmonate may be supplied at a concentration of 50 to 500 uM.

The methyl jasmonate can be supplied one week before harvest.

The methyl jasmonate may be supplied by an irrigation treatment or a side treatment.

The cultivation method of ginseng may include irradiating the ginseng with an LED mixed with red light and blue light.

The mixing ratio of the red light and the blue light may be 10: 1 to 7: 3.

The amount of light of the LED may be 50 to 150 mu mol.

The ginseng cultivation method may further include the step of irradiating the ginseng with blue light or UV-A.

The ginseng cultivation method may include supplying the ginseng with a culture at a temperature of 18 to 21 ° C and a pH of 5.5 to 6.5.

The electrical conductivity of the culture medium may be 0.3-0.5 mS / cmm in the early stage and 0.7-1.0 mS / cm in the flowering stage after the flowering period.

Another embodiment of the present invention provides ginseng grown by the above method.

Another embodiment of the present invention provides a food composition comprising an extract obtained from ginseng grown by the above method.

The ginseng cultivated according to the present invention can increase the content of ginsenoside and saponin. More specifically, the content of ginsenosides and saponins such as Rg1, Re, Rg2, F2, and K can be increased. Thus, pharmacological effects such as cancer prevention, cancer cell growth inhibition, blood pressure lowering, protection of brain cells, and improvement of learning ability can be expected.

The ginseng cultivated according to the present invention can increase the content of ginsenoside and saponin in both leaves, stems, and roots. In addition, the content of ginsenoside and saponin can be increased when the side treatment of methyl jasmonate is performed, and when the amount of ginsenoside and saponin is higher than that of methyl jasmonate when irradiated with blue light or UV-A Can be increased.

In addition, the ginseng cultivated according to the present invention can increase the content of ginsenoside and saponin, thereby providing a food composition containing the extract obtained from the ginseng. In such a food composition, it is possible to prevent cancer, Depression, cranial nerve cell protection and improvement of learning ability.

FIG. 1 is a photograph of ginseng grown in a common soil and ginseng grown in accordance with an embodiment of the present invention.
FIG. 2 is a graph showing ginsenoside contents of ginseng grown in general soil and control group.
FIG. 3 is a graph showing the saponin content of ginseng grown in a common soil and a control group.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Therefore, the embodiments of the present invention can be modified into various other forms, and the scope of the present invention is not limited to the following embodiments.

Throughout the description of the present invention, when a component is referred to as " comprising ", it means that it can include other components as well, without excluding other components unless specifically stated otherwise.

In the specification of the present invention, it is to be understood that when a step is located "on" or "before" another step, this is not only the case where a step is in a direct time series relationship with another step, And may have the same rights as in the case of an indirect temporal relationship in which the temporal order of the two phases can be changed.

The terms " about ", " substantially ", etc. used to the extent that they are used throughout the specification of the present invention are used in their numerical values or in close proximity to their numerical values when the manufacturing and material tolerances inherent in the meanings mentioned are presented, Is used to prevent unauthorized exploitation by an unscrupulous infringer of precise or absolute disclosures in order to aid in the understanding of the disclosure. The term " step " or " step of ~ " used throughout the specification does not mean " step for.

The term " active ingredient " as used herein in the context of the present invention may mean, for example, the amount of drug or pharmaceutical agent to elicit the biological or medical response of a tissue, system, animal or human being sought by a researcher or clinician . Also, the amount effective to promote normal physiological function may be included within its scope.

The present invention relates to a ginseng cultivation method and a ginseng cultivated by the method, wherein the ginseng cultivation method according to the present invention comprises: seeding the seedling ginseng in a box at 15 to 20 ° C and filling it into a hydroponic cultivation facility; Adjusting the temperature of the ginseng seedling to a temperature range of 15 to 20 ° C and a humidity range of 80 to 90%; Adjusting the temperature of the ginseng to a temperature range of 21 to 25 ° C and a humidity range of 65 to 75%; And feeding the ginseng with methyl jasmonate.

The ginseng cultivated by the ginseng cultivation method according to the present invention can increase the content of saponin.

In addition, one embodiment of the present invention provides a food composition comprising an extract obtained from ginseng cultivated according to the present invention.

Hereinafter, the ginseng cultivation method according to the present invention will be described in detail.

According to one embodiment of the present invention, temperature and humidity can be controlled during cultivation of ginseng. In addition, ginseng can be cultivated by hydroponic cultivation in order to smoothly supply methyl jasmonate. Hydroponic cultivation means a method of growing plants in a culture medium made of water and water-soluble nutrients without using soil.

According to one embodiment of the present invention, the ginseng can be cultivated in the facility having the irrigation facility, and the related facility can be installed in the facility for the temperature and humidity control.

According to one embodiment of the present invention, it is possible to seed the seedlings directly by sowing in facilities having irrigation facilities, or by cultivating seedlings grown intensively in a greenhouse or a vinyl house into hydroponic cultivation facilities equipped with irrigation facilities.

For example, seedling seeds can be seeded in a box at a temperature of 15 to 20 ° C, and then seeded into hydroponic cultivation facilities.

According to one embodiment of the present invention, the box may be enclosed and maintained at a temperature of 15-20 ° C. In addition, the humidity can be maintained at 90 to 100%.

After the plant is planted in hydroponic cultivation facility, the stem can be controlled at 15 ~ 20 ℃ for later development and 21 ~ 25 ℃ for later development.

If the temperature is outside the range of 15 to 20 占 폚, there is a possibility that cold weather or disease may occur. In addition, when the growth period is out of the range of 21 to 25 占 폚, there is a fear that growth is inhibited or high temperature damage is caused.

Although not limited thereto, a fan device can be used for temperature control.

In addition, it is possible to keep the humidity slightly higher to 80 ~ 90% after the planting, and the germ of the germinating leaves is developed. Although not limited thereto, the martial art may be 7 to 10 days.

Also, ginseng germination can germinate and maintain the humidity from 65 to 75% after leaf spreading.

If the humidity is out of the range of 80 to 90%, the growth of seedlings may be inhibited. In addition, when the growth period is out of the range of 65 to 75%, growth may be inhibited or disease may occur.

Although not limited thereto, a fan device may be used for humidity control.

In addition, according to one embodiment of the present invention, a culture broth can be provided to supply nutrition during cultivation of ginseng.

The type of the culture solution is not particularly limited, and a culture solution that is conventionally used in the hydroponic culture of ginseng can be used.

According to an embodiment of the present invention, the culture liquid may be supplied at a temperature of 18 to 21 DEG C, and the pH of the culture liquid may be 5.5 to 6.5.

In addition, the electrical conductivity (EC) of the culture liquid may be 0.3 to 1.0 mS / cm. More specifically, it may be 0.3 to 0.5 mS / cmm for the entire shoot period in the emergence period and 0.7 to 1.0 mS / cmm after the flowering period. When the electric conductivity is out of the range, there is a fear that the growth is lowered.

Further, according to one embodiment of the present invention, the supply of the culture medium can be stopped and only the water can be supplied one week before harvest.

Also, according to one embodiment of the present invention, methyl jasmonate can be supplied to ginseng. Methyl jasmonate can be represented by the following formula.

[Chemical Formula]

Methyl jasmonate can increase the saponin content by promoting the expression of genes that produce saponin in a concentration-dependent manner.

The methyl jasmonate may be supplied at a concentration of 50 to 500 uM.

According to one embodiment of the present invention, the method of supplying the methyl jasmonate is not particularly limited and may be supplied, for example, by an irrigation treatment or a side treatment. The side surface treatment can be carried out by spraying the methyl jasmonate solution with an atomizer.

The methyl jasmonate may be supplied from one week before harvest, and may be fed from one to three times a day.

According to an embodiment of the present invention, the ginseng may be irradiated with an LED in which red light and blue light are mixed. Thus, the growth of ginseng can be promoted.

The red light and the blue light may be mixed at a ratio of 10: 1 to 7: 3, and specifically, 8: 2 to 7: 3. If the mixing ratio of the red light and the blue light is out of the above range, the growth promoting effect may be insufficient.

In addition, according to an embodiment of the present invention, the light amount of the LED may be 50 umol to 150 umol.

When the ratio of the amount of light is out of the range of 50 mu mol to 150 mu mol, the growth may be inhibited by a low light amount or a high light amount.

Also, according to an embodiment of the present invention, the LED light irradiation may be performed for 60 seconds at intervals of 10 minutes, and the LED light irradiation may not be performed for 20 minutes at 60 seconds. This regulation can help to absorb respiration and nutrients in the roots.

According to an embodiment of the present invention, the ginseng may be irradiated with blue light or UV-A. The irradiation of blue and / or UV-A may be carried out one week before harvest, though not limited thereto. In such a case, the content of saponin and the like of ginseng can be increased.

The content of ginsenosides and saponins such as Rg1, Re, Rg2, F2, and K can be increased in the ginseng cultivated according to one embodiment of the present invention. Thus, pharmacological effects such as cancer prevention, cancer cell growth inhibition, blood pressure lowering, protection of brain cells, and improvement of learning ability can be expected.

Further, another embodiment of the present invention provides a food composition comprising an extract obtained from ginseng cultivated by the above method. The food composition according to one embodiment of the present invention includes an extract of ginseng as an active ingredient, and the ginseng cultivated according to the present invention may be used.

The method of obtaining the extract from the ginseng cultivated by the above method is not particularly limited, and the extract can be obtained by a known method. The extract may be obtained in the form of a powder or an extract.

In the present invention, the term 'food' means a natural product or a processed product containing one or more nutrients, preferably a state of being able to be eaten directly through a certain degree of processing, Which may include foods, food additives, functional foods, nutritional supplements, health supplements, food additives and beverages.

According to one embodiment of the present invention, the food composition may be used as a raw material, an additive, or a food additive of food.

In addition, according to one embodiment of the present invention, the food composition can be used alone or in combination with other food or food ingredients, and can be suitably used in a method in which a food composition is usually used.

The content of the food composition may suitably be determined according to the intended use (prevention, health or therapeutic treatment). For example, the ginseng extract may be contained in an amount of 0.01 to 20% by weight based on the total weight of the food during the manufacture of food or beverage.

Examples of foods that may include food compositions according to one embodiment of the present invention include, but are not limited to, various foods such as beverages, gums, tea, vitamin complexes, Powders, granules, precipitates, tablets, capsules or beverages.

According to one embodiment of the present invention, the health supplement functional food may be a health functional beverage, and the beverage may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the natural carbohydrate include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Flavoring agents (saccharin, aspartame, etc.) other than those described above may also be used.

According to one embodiment of the present invention, the health-aid functional food includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (such as cheese and chocolate) Alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

Hereinafter, the present invention will be described in detail with reference to specific examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

[Example]

Ginseng cultivation

1) Temperature management

Ginseng was cultivated in a hydroponic culture system in a facility equipped with a fan device. The temperature was controlled at 15 ~ 20 ℃ during the emergence stage and 21 ~ 25 ℃ during the growing season.

2) Humidity management

Humidity was maintained at 80 ~ 90% for 7 ~ 10 days, and the germination of ginseng germinated at 70 ± 5% after leaf emergence.

3) Light management

The light was irradiated at a light amount of about 50 to 150 μmol using an LED in which a red light having a wavelength of 660 nm and a blue light having a wavelength of 450 nm were mixed at a ratio of 10: 1.

4) Management of culture medium

The temperature of the culture medium was adjusted to 18 to 21 ° C, the pH was 5.5 to 6.5, the EC was 0.4 mS / cmm at the early stage and 0.7 to 1.0 mS / cmm after the flowering period. One week before harvest, the culture was stopped and only water was supplied. The watering method was watering for 60 seconds at 10 minute intervals when the LED was turned on and 60 seconds at 20 minute intervals when the LED was turned off.

5) Supply of methyl jasmonate (MeJA)

Example  One( MeJA Irrigation treatment )

From 1 week before harvesting, the plants were treated with 100 μM water.

Example  2( MeJA  Side processing)

One week before harvesting, the cells were treated with 100 uM concentration three times a day (breakfast lunch and dinner).

Example  3 ( MeJA  Side processing + Blue light  Research)

From 1 week before harvesting, the plants were treated with 100uM concentration three times a day (breakfast lunch and dinner), and blue light was irradiated 1 week before harvest.

Example  4( MeJA  Side treatment + UV-A)

From 1 week before harvesting, 100 uM concentration was applied to the sides 3 times a day (morning and evening), and UV-A was irradiated 1 week before harvest.

[evaluation]

1. Component analysis

1) and 1-year and 6-year-old ginseng cultivated in the general soil, and 2-year-old ginseng grown under the same conditions as the above-mentioned example but not irradiated with methyl jasmonate and LED light as a control group, The content was measured by commissioning the analysis center, and the results are shown in Table 1 below.

Gensenosides Contents Soil Control group Soil 1Y (Root) 2Y (Leaf) 2Y (Stem) 2Y
(Root) 6Y (Root) Rg1 0.24 3.26 1.12 0.92 0.44 Re 0.89 10.45 1.49 0.89 0.55 Rg2 (S) 0.09 1.13 0.07 0.1 0.04 F2 0 2.23 0.1 0.03 0 K 0 0.02 0 0.02 0 Saponin 1.98 18.94 3.29 3.14 1.92

                                         Unit: mg / g

FIG. 1 is a photograph of ginseng grown in a common soil and ginseng grown in accordance with an embodiment of the present invention.

FIG. 2 is a graph showing ginsenoside contents of ginseng grown in a general soil and a control group, and FIG. 3 is a graph showing saponin contents of ginseng grown in a common soil and a control group.

Referring to Table 1, FIG. 2, and FIG. 3, ginsenoside and saponin contents in leaf, stem and root of ginseng cultivated in a controlled temperature and humidity condition were increased compared with ginseng grown in general soil .

2) Leaves, stems and roots of the ginseng cultivated according to the above examples were collected to analyze contents of ginsenosides and saponins, and the results are shown in Tables 2 and 3 below.

division Example 2
(MeJA (Side View)) Example 1
(MeJA) 2Y (Leaf) 2Y (Stem) 2Y (Root) 2Y (Leaf) 2Y (Stem) 2Y (Root) Saponin 151.52 9.87 18.84 56.82 9.87 12.56

division Example 3
(MeJA (side view) + blue light) Example 4
MeJA (side) + UV-A 2Y (Leaf) 2Y (Stem) 2Y (Root) 2Y (Leaf) 2Y (Stem) 2Y (Root) Saponin 196.976 11.844 24.492 212.128 12.831 25.434

Comparing Tables 1 to 3, the content of ginsenoside and saponin in leaves, stems and roots of ginseng cultivated in a controlled temperature and humidity condition was increased as compared with ginseng grown in general soil, It was confirmed that the content of ginsenoside and saponin in the control group was smaller than those in the examples. That is, in the case of the ginseng cultivated according to the embodiment of the present invention, the content of ginsenoside and saponin in leaves, stems, and roots increased sharply. More specifically, in the case of ginseng leaves, it was increased about 3 to 15 times as compared with that of the control, and it was confirmed that about 2 to 4 times in the case of the ginseng stem and about 3 to 6 times in the case of the ginseng root. In addition, it was confirmed that the content of ginsenoside and saponin was further increased when the side treatment of methyl jasmonate was performed. In addition, it was confirmed that the content of ginsenoside and saponin was further increased when blue light or UV-A was irradiated with methyl jasmonate.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but many variations and modifications may be made without departing from the scope of the present invention. It is evident that many variations are possible by those skilled in the art. It will be apparent to those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. something to do.

Claims (12)

Seeding the seedlings in a box at a temperature of 15 to 20 ° C, and then filling the seedlings into hydroponic cultivation facilities;
Adjusting the temperature of the ginseng seedling to 15-20 ° C and a humidity range of 80-90%;
Adjusting the temperature of the ginseng to a temperature range of 21 to 25 ° C and a humidity range of 65 to 75%; And
Feeding the ginseng with methyl jasmonate;
≪ / RTI >
The method according to claim 1,
Wherein the methyl jasmonate is supplied at a concentration of 50 to 500 uM.
The method according to claim 1,
Wherein said methyl jasmonate is supplied from one week before harvest.
The method according to claim 1,
Wherein the methyl jasmonate is supplied by an irrigation treatment or side treatment.
The method according to claim 1,
And irradiating the ginseng with an LED mixed with red light and blue light.
6. The method of claim 5,
Wherein the mixing ratio of the red light and the blue light is from 10: 1 to 7: 3.
6. The method of claim 5,
Wherein the light amount of the LED is in the range of 50 to 150 mu mol.
The method according to claim 1,
Further comprising the step of irradiating the ginseng with blue light or UV-A.
The method according to claim 1,
Wherein the ginseng is cultivated at a temperature of 18 to 21 캜 and a pH of 5.5 to 6.5.
10. The method of claim 9,
The electrical conductivity of the cultured liquid is 0.3 to 0.5 mS / cmm in the early stage and 0.7 to 1.0 mS / cmm after the flowering stage in the emergence stage.
10. Ginseng grown by the method of any one of claims 1 to 10.
A food composition comprising an extract obtained from ginseng grown by the method of any one of claims 1 to 10.

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