KR20220107654A - Method for cultivating hydroponic-cultured Panax ginseng for increasing ginsenoside content and the use of it - Google Patents

Abstract

The present invention relates to a method for cultivating hydroponic-cultured Panax ginseng, which comprises a pre-harvest treatment step of performing treatment with geraniol before harvest. Accordingly, it is possible to obtain hydroponic-cultured Panax ginseng having 10 % or higher of the total content of ginsenoside than hydroponic-cultured Panax ginseng grown in the same cultivation method and not subjected to pre-harvest treatment.

Description

Method for cultivating hydroponic-cultured Panax ginseng for increasing ginsenoside content and the use of it}

The present invention relates to a cultivation method of hydroponic ginseng, the use of hydroponic ginseng prepared therefrom, and more particularly, to a cultivation method of hydroponic ginseng to increase the ginsenoside content by pre-harvest treatment, and to the use of hydroponic ginseng prepared therefrom will be.

Ginseng is a perennial, shady herbaceous plant belonging to the genus Panax of the Araliaceae family, and has been used as an important medicinal material in oriental medicine for a long time. Ginseng is effective in treating various diseases and promoting recovery from diseases, and extensive research is being conducted to explore the pharmacological components and pharmacological effects of ginseng.

The representative effects scientifically discovered so far include the maintenance of homeostasis of body control functions, and based on these actions, anti-fatigue and anti-stress action, anti-diabetic action, blood pressure control action, anti-cancer action, prevention of arteriosclerosis and hypertension, brain Functional enhancement, gastrointestinal function enhancement, immune function enhancement, antiviral action, etc. have been reported.

The first scientific research on the composition of ginseng began with the separation of an amorphous glycoside mixture in the United States and naming it Panaquilon. It has been scientifically proven that ginseng contains ingredients with various pharmacological effects. This action is mainly known to be caused by ginseng glycosides, that is, ginsenosides, a saponin component contained in ginseng. Other active ingredients include sapogenin, poly Acetylene, pyrazine derivatives, maltol, and the like are known. Ginsenoside, the main ingredient of ginseng efficacy, is mainly in the form of dammarane non-glycosides and triterpene glycosides, which are composed of several saccharides with different numbers, and about 40 types are known so far. It is reported that different ginsenoside components contained in ginseng have various medicinal effects.

However, natural medicinal ginseng has to be cultivated for a long period of 4 to 6 years in a cool highland with good drainage, and is not only affected by the natural climate, but is also an uneconomical crop that cannot be continuously cultivated in the same place. Therefore, research on a cultivation method that can easily obtain the pharmacological components of ginseng is being conducted.

Korean Patent No. 10-2035590

An object of the present invention is to provide a cultivation method of hydroponic ginseng using geraniol and blue light treatment, which can improve the content of ginsenosides in cultivating hydroponic ginseng.

Another object of the present invention is to provide a food composition, a cosmetic composition, and a feed composition comprising, as an active ingredient, hydroponic ginseng grown according to the cultivation method of hydroponic ginseng using geraniol and blue light treatment.

According to one aspect of the invention,

A method of cultivation of hydroponic ginseng comprising; a pre-harvest treatment step of treating geraniol before harvesting is provided.

In the pre-harvest treatment step, blue light treatment may be additionally performed together with the geraniol treatment.

The blue light treatment may be performed in a bright state in which white light is irradiated in a photoperiod including a bright state and a dark state.

The geraniol treatment may be performed according to foliar application.

In the geraniol treatment, the geraniol may be treated at a concentration of 100 to 1000 ppm.

The geraniol (Geraniol) treatment may be performed 6 to 8 days before harvest.

Prior to the harvest pretreatment step,

(a) preparing seedlings in a frozen state; (b) gradually increasing the temperature of the seedlings in the frozen state to acclimatize, then washing and disinfecting; (c) the washing and disinfecting step; (d) a planting step of planting the seedlings that have undergone the seedling step; and (e) a cultivation step of cultivating seedlings that have undergone a formal step.

According to another aspect of the present invention,

There is provided hydroponic ginseng, which is grown according to the cultivation method, and characterized in that ginsenosides are increased by 10% or more compared to hydroponic ginseng that is not pre-harvested.

The cultivation method of hydroponic ginseng using geraniol and blue light treatment of the present invention has the effect of increasing the ginsenoside content of roots and leaf stems by 10% or more compared to the cultivation method of hydroponic ginseng without pre-harvest treatment. .

1 is a photograph of seedlings of Example 1 (1) seedling preparation step.
2 is a photograph of seedlings in the (5) seeding stage of Example 1.
3 is a photograph of ginseng seedlings in the (6) planting stage of Example 1.
Figure 4 is a photograph of hydroponic ginseng of Example 1 (8) cultivation step (when 15-28 days have elapsed).
5 is a photograph of hydroponic ginseng of Example 1 (8) cultivation step (29-42 days elapsed).
6 is a photograph of hydroponic ginseng of Example 1 (8) cultivation step (43 to 84 days elapsed).
7 is a photograph of hydroponic ginseng after (9) harvest pre-treatment of Example 1.
8 is a photograph of hydroponic ginseng after harvesting (10) of Example 1.

Hereinafter, the cultivation method of hydroponic ginseng to increase the ginsenoside content of the present invention will be described.

The cultivation method of hydroponic ginseng to increase the ginsenoside content of the present invention is characterized in that it comprises the step of treating with geraniol (Geraniol) before harvesting.

In the pre-harvest treatment step, blue light treatment may be additionally performed together with the geraniol treatment.

When only geraniol treatment is performed, it may be more advantageous to increase the ginsenoside content of the root part of hydroponic ginseng, and when blue light treatment is additionally performed together with geraniol treatment, ginseno of the leaf stem of hydroponic ginseng It may be more advantageous to increase the side content.

Therefore, the method of pre-harvest treatment can be selected according to the needs of the leaf stem, root, and total use of hydroponic ginseng.

In general, the plant cultivation method using nutrient solution is a hydroponics method in which plants are grown using liquid nutrient solution. have.

Using geraniol of the present invention and blue light treatment The cultivation method of hydroponic ginseng is preferably carried out according to the spray method cultivation method.

The aeroponic cultivation method is an aeroponics method for cultivating plants using a spray nutrient solution formed using a general sprayer, and a fog phonics method for growing plants using a spray nutrient solution formed using an ultrasonic module ( It can be divided into fogponics method. Since the particles of the nutrient solution used in Fogphonics are smaller than those of Aerophonics, the nutrient solution sprayed in Fogphonics is affected by gravity relatively less than Aerophonics, so it can supply the nutrient solution to the entire planted plant relatively evenly. there are advantages to A fog phonics device, that is, a nutrient solution in a method of spraying a nutrient solution using an ultrasonic vibration module, is generally a liquid nutrient solution stored in a cultivation bed in which plants are planted and grown, and ultrasonic waves provided in the cultivation bed It may be configured in a form in which the liquid nutrient solution is sprayed by the vibration module.

If necessary, the aeroponic cultivation method can be used by selecting an aerophonics method or a pokphonics method, and it is more preferable to use the aerophonics method for the hydroponic ginseng cultivation method of the present invention.

Geraniol is a monoterpenoid and alcohol represented by the following formula (1). It is the main component of rose oil, palmarosa oil, and citronella oil, and is also contained in small amounts in various essential oils such as geranium and lemon. Although insoluble in water, it is readily soluble in most common organic solvents and appears as a colorless or pale yellow oil. Geraniol has a rose-like scent and is also used to flavor peaches, raspberries, grapefruit, apples, plums, limes, oranges, lemons, watermelons, pineapples, and blueberries.

[Formula 1]

The geraniol treatment is preferably foliar application at a concentration of 100 to 1000 ppm, more preferably 200 to 800 ppm, even more preferably 400 to 600 ppm concentration.

The blue light treatment is preferably performed in a bright state in which white light is irradiated in a photoperiod including a bright state and a dark state.

The blue light may have a wavelength range of 430 to 470 nm, more preferably 440 to 460 nm, and even more preferably, 450 nm of blue light.

The amount of light of the blue light is preferably 50 to 150 μmol m ^-2 s ^-1 , more preferably 80 to 130 μmol m ^-2 s ^-1 , even more preferably 100 to 120 μmol m ^-2 s ^-1 .

Geraniol treatment before the harvest is preferably performed 6 to 8 days before harvest, more preferably 6.5 to 7.5 days before, even more preferably 7 days before.

Prior to the pre-harvest treatment step, hydroponic ginseng can be grown sequentially according to the steps below.

First, prepare frozen ginseng seedlings (step a).

Next, the temperature of the seedlings in the frozen state is gradually increased to acclimatize, and then washed and disinfected (step b).

Thereafter, the washing and disinfecting step undergoes a seedling step of keeping the rough seedlings covered with vinyl to maintain a relative humidity of 70 to 90% in a seedling box (step c).

Next, a planting stage of planting seedlings that have passed through the seedling stage is performed (step d).

In this step, the pH of the nutrient solution may be 5.8 to 6.2.

The EC of the nutrient solution in the planting stage is preferably 0.15 to 0.25 dS/m.

In addition, the irrigation cycle is preferably every 12 to 17 minutes for 30 to 100 seconds, more preferably every 13 to 16 minutes for 40 to 80 seconds, even more preferably every 15 minutes for 50 to 70 seconds. can do.

It is preferable to additionally perform the step of removing and planting an individual with poor leaf growth or disease between 1 to 7 days after the planting step.

Thereafter, the cultivation step of cultivating seedlings that have undergone the formal step is performed (step e).

In the early stage of the cultivation stage, it is preferable to set the irrigation cycle under the same conditions as in the planting stage, and then gradually increase the irrigation interval to irrigate every 18 to 20 minutes in the middle of cultivation, and every 28 to 32 minutes in the late cultivation.

The pH of the nutrient solution in the cultivation step is preferably 5.8 to 6.2.

In addition, the EC of the nutrient solution should be gradually increased to 0.15 to 0.20 dS/m immediately after planting, 0.35 to 0.45 dS/m at the beginning of cultivation, 0.65 to 0.75 dS/m during the middle of cultivation, and 0.95 to 1.05 dS/m at the end of cultivation. desirable. When this nutrient solution EC is set, the growth of hydroponic ginseng can be achieved most efficiently.

It is preferable that the photoperiod from seedling to harvest repeat light and dark with a cycle of 11 to 13 hours, preferably a cycle of 11.5 to 12.5 hours, more preferably a cycle of 12 hours.

According to the present invention, hydroponic ginseng cultivated by the above cultivation method, treated with geraniol or treated with geraniol and blue light before harvest, has dried roots, leaf stems or hydroponic ginseng, compared to hydroponic ginseng that has not been pre-harvested. With respect to the whole ginseng, the total content of ginsenosides may be increased by 10% or more based on the dry weight of hydroponic ginseng. Preferably it may be increased by 10 to 30%, more preferably by 10 to 20%.

The present invention provides a method for producing a ginsenoside extract, characterized in that the ginsenoside is extracted from hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content.

The ginsenoside extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. In this case, the type of the organic solvent used or the mixing ratio of water and the organic solvent is not particularly limited.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohol, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol may be used as the lower alcohol. In addition to these organic solvents, polar solvents such as acetic acid, dimethyl-formamide (DMFO), dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, and decane , cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene , benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and non-polar solvents such as tetrahydrofuran (THF) may be used.

The ginsenoside extract may be a water extract, an ethanol extract, a methanol extract, or an extract using a mixed solvent of any two or more solvents selected from water, ethanol and methanol.

The preparation of the water extract can be prepared by extracting hydroponic ginseng with increased ginsenoside content by the method of the present invention for 2 to 48 hours at 40 to 100 ° C. In order to increase the extraction efficiency, it can be prepared by extraction with water added with one or more enzymes selected from the group consisting of cellulase, biscozyme, alcalase and fexin at 40 to 55° C. for 2 to 24 hours.

The preparation of the ethanol extract or ethanol aqueous solution extract is 2 to 36 hours at 20 to 60 ° C., preferably 40 to 40 vol. It can be prepared by extraction at ~ 50 °C for 2.5 to 6 hours, and more preferably by extraction with 70% by volume of an ethanol aqueous solution at 45 °C for 3 hours.

As used herein, the term 'extract' also includes a fraction obtained by additionally fractionating the extract. That is, the ginsenoside extract includes not only those obtained using the above-described extraction solvent, but also those obtained by concentrating ginsenosides by additionally applying a purification process here. In addition, the fraction obtained by passing the extract or fraction through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the ginsenoside extract of the present invention.

The present invention provides a food composition containing hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content or a food composition containing a ginsenoside extract prepared by the above method.

When hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content according to the present invention or a ginsenoside extract extracted therefrom is used as an active ingredient additive in a health functional food or general food, the hydroponic ginseng according to the present invention or The ginsenoside extract can be added as it is or used with other foods or food ingredients, and can be used appropriately according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to each purpose of use, such as prevention, health or treatment.

In general, when preparing food or beverage, the hydraulic ginseng or ginsenoside extract according to the present invention may be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term ingestion for health and hygiene or health control, the amount may be less than the above range, and the present invention uses a natural product or an extract therefrom, so there is no problem in terms of safety. It can also be used in an amount beyond the range.

The type of food is not particularly limited, and examples of food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and dairy products including ice cream. , various soups, beverages, teas, drinks, alcoholic beverages, and vitamin complexes, and may include all foods in a conventional sense.

Among the health functional foods according to the present invention, beverage foods may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The above-mentioned natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatine and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate may be about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the functional food according to the present invention.

In addition to the above, the food composition includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may contain a carbonation agent used for In addition, the food composition of the present invention may contain flesh for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not limited, but is generally selected in the range of 0.01 to 0.1 parts by weight relative to 100 parts by weight of the composition of the present invention.

The present invention provides a feed composition comprising hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content or a feed composition comprising a ginsenoside extract prepared by the above method. The feed composition may be a feed or feed additive.

The feed composition includes organic acids such as citric acid, humic acid, adipic acid, lactic acid, and malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate), polyphenol, catechin, alpha-tocopherol , rosemary extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, any one or more of natural antioxidants such as phytic acid may be further included.

As an auxiliary component of the feed composition, various auxiliary substances such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal enzymes, and live microorganism preparations are grains, for example, pulverized or crushed wheat, oats, barley, corn and rice; plant protein feeds, such as those based on rape, soybean and sunflower; animal protein feeds such as blood meal, meat meal, bone meal and fish meal; After mixing all the dry ingredients and dry additives consisting of sugar and dairy products, for example, various milk powder and whey powder, a liquid component and a component that becomes liquid after heating, that is, a lipid, for example, an animal product optionally liquefied by heating In addition to the main ingredients such as fats and vegetable fats, it can be used together with substances such as nutritional supplements, digestion and absorption enhancers, growth promoters, and disease prevention agents.

The feed composition may be administered alone to the animal in combination with other feed additives in an edible carrier.

The feed composition can be easily administered as a top dressing or by mixing them directly into the animal feed or separately from the feed, in a separate oral formulation, by injection or transdermally, or in combination with other ingredients. Typically, a single daily dose or divided daily doses may be used as is well known in the art.

When the feed composition is administered separately from the animal feed, the dosage form of the composition can be combined with their non-toxic pharmaceutically acceptable edible carriers to prepare an immediate release or sustained release formulation, as is well known in the art. Such edible carriers may be solid or liquid, for example corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol. When a solid carrier is used, the dosage form of the compound may be a tablet, capsule, powder, lozenge or sugar-containing tablet or top dressing in microdispersible form. When a liquid carrier is used, it may be in the form of a soft gelatin capsule, or a syrup or liquid suspension, emulsion or solution. The dosage form may also contain adjuvants such as preservatives, stabilizers, wetting or emulsifying agents, solution promoters, and the like.

The feed composition may be used by adding to the animal feed by immersion, spraying or mixing.

The feed composition can be applied to a number of animal diets including mammals, poultry and fish. More specifically, the diet includes mammals of commercial importance, such as pigs, cattle, sheep, goats, laboratory rodents (rat, mouse, hamster and gerbil), fur-bearing animals (eg mink and fox), and It can be used on zoo animals (eg monkeys and tailless monkeys), as well as livestock (eg cats and dogs). Commonly commercially important poultry include chickens, turkeys, ducks, geese, pheasants and quails. Commercially raised fish such as trout may also be included.

In the method for compounding animal feed including the feed composition, the composition is mixed with the animal feed in an amount of about 1 g to 100 g per 1 kg of feed on a dry weight basis. In addition, after the feed mixture is thoroughly mixed, a hard viscous granular or granular material is obtained depending on the degree of pulverization of the ingredients. It is supplied as a mash or formed into desired discrete shapes for further processing and packaging. At this time, in order to prevent segregation during storage, it is preferable to add water to the animal feed, followed by a conventional pelletizing, inflating, or extruding process. Excess water can be dried off.

The present invention provides a cosmetic composition comprising hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content or a cosmetic composition comprising a ginsenoside extract prepared by the above method.

The cosmetic composition of the present invention may be prepared in the form of a lotion (skin), lotion, essence, cream, pack, or the like.

In the formulation, hydroponic ginseng grown according to the cultivation method for increasing the ginsenoside content according to the present invention or a ginsenoside extract extracted therefrom is used in an amount of 0.1% by weight or more, preferably 0.2 to 50% by weight, more preferably 0.3 ~ 30% by weight, most preferably 0.5 to 10% by weight may be used

The cosmetic composition may further include an appropriate carrier, excipient or diluent commonly used in the manufacture of cosmetics.

The carrier, excipient or diluent includes purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, humectant, thickening agent, antioxidant, viscosity stabilizer, chelating agent , buffers, lower alcohols, and the like. If necessary, whitening agents, moisturizing agents, vitamins, sunscreen agents, perfumes, dyes, and antibacterial agents may be further included.

Hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil, and avocado oil may be used as the oil. As the wax, beeswax, spermaceti, carnauba, candelilla, montan, ceresin, liquid paraffin, and lanolin may be used. can be used As the fatty acid, stearic acid, linoleic acid, linolenic acid, and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyl dodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol, and hexadecanol may be used. As the ester, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used. Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate, sodium N-acyl glutamate; cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, sucrose fatty acid ester, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethanol nonionic surfactants such as monoethanol arnide, polyoxypropylene glycol, polyoxyethylene castor oil, polyoxyethylene lanolin, and the like. Glycerin, 1,3-butylene glycol, and propylene glycol may be used as the desiccant, and ethanol and isopropanol may be used as the lower alcohol. Examples of thickeners include sodium alginate, sodium caseinate, gelatin agar, xanthan gum, starch, cellulose ethers (eg, hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, hydroxypropylmethyl cellulose), polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol and sodium carboxymethyl cellulose; and the like. As antioxidants, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, and ethoxyquin are available. As chelating agents, disodium edetate and ethanehydroxy diphosphate are available. citric acid, sodium citrate, boric acid, borax, and disodium hydrogen phosphate are available as buffers.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

[Example]

Example 1: Ginsenoside-increased hydroponic ginseng cultivation according to geraniol treatment

(1) Preparation of seedlings

1. Prepare seedlings that meet the following conditions. A photograph of the prepared seedlings is shown in FIG. 1 .

- With a weight of 0.8g or more, the brain is full

- No wounds or signs of nematode damage on the brain head or body

2. For long-term storage of seedlings, they are stored using vermiculite, and the storage conditions are as follows.

- Precooled at 4 to 6°C for the first 2 weeks.

- Then stored at 3 ℃ for 3 days and stored at -1 ℃ for 4 days.

- The final temperature was maintained at -5℃ and stored.

(2) Purification of seedlings

The seedlings stored at -5°C for 3 days are stored at -1°C for 3 days, then at 3°C for 3 days, then at 7°C for 4 days, and at 10°C for the last 4 days to acclimatize the seedlings.

(3) Washing ginseng seedlings

The purified seedlings were washed in running water to remove impurities such as soil and medium, and wounds, tenderness, and infected strains were also removed, taking care not to injure the roots.

(4) Disinfection of seedlings

The washed seedlings were sterilized by immersion in lime Bordeaux solution of 1,000ppm concentration for 10 minutes.

(5) Seedling

The sterilized seedlings were arranged in a seedling box and covered with plastic to maintain humidity. A photograph of seedlings of this stage is shown in FIG. 2 .

(6) set meal

A sponge for planting sterilized by immersion in lime Bordeaux solution of 1,000 ppm concentration was prepared, and germinated seedlings that had undergone the seedling stage were planted here. The state of seedlings in the planting stage is shown in FIG. 3 .

At this time, as shown in the photo, the fixing part for holding and fixing the individual sponge was the upper part of the seedling. That is, the lower part of the root is exposed to the bottom of the regular plate, and the stem is exposed to the top of the regular plate.

(7) Bosik

Between 1 to 7 days after planting, the individual with poor leaf growth or disease was removed and transplanted.

(8) cultivation

(1 to 14 days after formality)

At 14 days after planting, lime Bordeaux solution was added to water to disinfect at a concentration of 1,000 ppm. After sterilizing for 5-10 minutes and leaving it for 2-3 hours, fresh water was supplied.

(15 ~ 28 days after formality)

When 28 days have elapsed after planting, sterilization was performed in the same manner as above with lime Bordeaux solution having a concentration of 1,000 ppm. A picture of hydroponic ginseng at this stage is shown in FIG. 4 .

(29 ~ 42 days after the official ceremony)

At 42 days after planting, sterilization was carried out in the same manner as above with lime Bordeaux solution having a concentration of 1,000 ppm. A photograph of hydroponic ginseng at this stage is shown in FIG. 5 .

(43 ~ 84 days after formality)

After 56, 70, and 84 days after planting, sterilization was carried out in the same manner as above with a lime Bordeaux solution having a concentration of 1,000 ppm at intervals of 2 weeks.

A photograph of hydroponic ginseng at this stage is shown in FIG. 6 .

(9) Harvest pretreatment

(After 43 days after official release)

The leaves were spray-treated with geraniol at a concentration of 500 ppm for 2 days 7 days before harvest. 7 shows a photograph of hydroponic ginseng subjected to pre-harvest treatment.

(10) Harvest

Harvested 1 week after pre-harvest treatment. A photograph of the harvested hydroponic ginseng is shown in FIG. 8 .

The indoor temperature, indoor relative humidity, nutrient solution pH, nutrient solution EC (Electrical Conductivity), irrigation cycle, water temperature, light quantity (white light) and photoperiod conditions are summarized in Table 1 below during the cultivation of hydroponic ginseng.

step Room temperature (℃) Indoor Humidity (%) Nutrient pH Nutrient EC (dS/m) irrigation cycle
(s/m) Water temperature (℃) Light quantity (μmol m ^-2 s ^-1 ) Photoperiod (light/dark)
(h/h) (5) Seedling 15-20 80±10 - - - - 64~110 12h/12h (6) Set meal time 19±2 80±10 6.0±0.2 0.20±0.05 60/15 17±2 64~110 12/12 (7) Feeding (1-7 days after meal) 19±2 80±10 6.0±0.2 0.20±0.05 60/15 17±2 64~110 12/12 (8) Cultivation (8-14 days after planting) 19±2 80±10 6.0±0.2 0.20±0.05 60/15 17±2 64~110 12/12 (8) Cultivation (15 ~ 28 days after planting) 19±2 70±10 6.0±0.2 0.40±0.05 60/20 17±2 64~110 12/12 (8) Cultivation (29 to 42 days after planting) 19±2 70±10 6.0±0.2 0.70±0.05 60/30 17±2 64~110 12/12 (8) Cultivation (43-84 days after planting) 19±2 70±10 6.0±0.2 1.00±0.05 60/30 17±2 64~110 12/12 (9) Harvest pretreatment 19±2 70±10 6.0±0.2 0.20±0.05 60/30 17±2 64~110 12/12

Example 2

(9) In pre-harvest treatment, foliar treatment with geraniol at a concentration of 500 ppm, and using a blue LED light source with a wavelength of 450 nm, blue light treatment with a light amount of 100 to 120 μmol m ^-2 s ^-1 was additionally performed. Hydroponic ginseng was grown under the same conditions as in Example 1, except that. The blue light treatment was performed together in the bright state irradiated with white light of the photoperiod.

Comparative Example 1

(9) Hydroponic ginseng was grown under the same conditions as in Example 1, except that pre-harvest treatment was not performed.

Comparative Example 2

(6) Geraniol treatment was not performed during harvest pretreatment, and only blue light treatment was performed with a light amount of 100 to 120 μmol m ^-2 s ^-1 using a blue LED light source having a wavelength of 450 nm. Hydroponic ginseng was grown under the same conditions as in Example 1.

[Experimental example]

Experimental Example 1: Measurement of ginsenoside content

70% (v/v) methanol was added to the dry powder of the leaf stem and the dry powder of the root of hydroponic ginseng grown according to Examples 1 and 2 and Comparative Examples 1 and 2, respectively, and adjusted to 50 ml, followed by an ultrasonic dispersing device ( JAC2010, KODO, Korea) was completely dissolved and filtered with a 0.45㎛ syringe filter. For the standard solution, take 1 mg of each standard sample to make 1 ml, prepare a standard stock solution, and then inject 100 μl of each standard stock solution and use LC/MS (Waters 2695, Waters micromass ZQ4000, USA) to use ginsenoside content (mg /g) were analyzed. Specifically, column XTerra®C18, temperature 40°C, mobile phase A: B = 20: 80 (A: 18% acetonitrile in 0.1% acetic acid, B: 80% acetonitrile in 0.1% acetic acid), flow rate 0.25 mL/min, injection The type and content of ginsenosides were measured under the condition of a volume of 5 μl.

The types and contents (mg/g) of ginsenosides contained in the hydroponic ginseng roots of Examples 1 and 2 and Comparative Examples 1 and 2 are summarized in Table 2 below, and the types of ginsenosides contained in the hydroponic ginseng leaf stem and The contents are summarized in Table 3 below. Here, the leaf stem of hydroponic ginseng includes the leaf and stem part except for the root.

division Control group (whole seedlings) Comparative Example 1 Comparative Example 2 Example 1 Example 2 pre-harvest treatment - unprocessed blue light geraniol geraniol + blue light Rg1 0.78 0.95 0.82 0.89 0.99 Re 5.55 4.75 4.98 5.59 4.71 Rf 0.45 0.47 0.42 0.49 0.43 Rh1 - - - - - Rg2 0.91 0.78 0.82 0.92 0.74 Rb1 2.61 2.46 2.69 2.88 2.78 Rc 1.08 1.03 1.35 1.40 1.34 Rb2 1.14 1.01 1.36 1.29 1.30 Rd 0.59 0.42 0.48 0.53 0.55 F2 - - - - - C-K 0.92 0.52 0.52 0.49 0.53 total amount 14.01 12.38 13.44 14.48 13.38 Relative amount based on seedlings (%) 100 88 96 103 95 Relative amount based on untreated group (%) - 100 109 117 108

According to the results of Table 2, when comparing the types and contents (mg/g) of ginsenosides contained in the hydroponic ginseng roots of Examples 1 and 2 and Comparative Examples 1 and 2, geraniol alone was treated as pre-harvest treatment Hydroponic ginseng of Example 1 showed the highest total amount of ginsenosides, and various types of ginsenosides showed overall high values. In the case of hydroponic ginseng root, Comparative Example 2 treated with blue light and Example 2 treated with geraniol + blue light had a similar total ginsenoside content.

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 pre-harvest treatment unprocessed blue light geraniol geraniol + blue light Rg1 11.03 11.53 10.41 12.32 Re 37.45 41.87 40.65 41.61 Rf 0.74 0.76 0.72 0.72 Rh1 0.10 0.11 0.09 0.14 Rg2 1.50 1.56 1.65 1.17 Rb1 0.85 1.03 1.20 1.14 Rc 1.72 2.37 2.41 2.82 Rb2 2.44 3.17 3.27 3.69 Rd 9.36 11.14 11.35 13.65 F2 12.54 12.25 10.94 11.58 C-K 0.86 0.83 0.83 0.83 total amount 78.58 86.60 83.53 89.66 Relative amount based on untreated group (%) 100 110 106 114

On the other hand, comparing the type and content (mg/g) of ginsenosides contained in the hydroponic ginseng leaf stem according to the results of Table 3, the hydroponic ginseng leaf of Example 2 performed in parallel with geraniol + blue light treatment as a pre-harvest treatment The stem showed the highest ginsenoside content. On the other hand, in the case of Example 1 treated with geraniol alone, the ginsenoside content was slightly lower than that of Comparative Example 2 treated with blue light, unlike the case of roots.

It was found that the leaf stem and root parts of cultivated hydroponic ginseng required slightly different conditions to maximize the ginsenoside content, respectively. Since it is economical to use both leaf stems and roots for hydroponically grown ginseng, the total ginsenoside content (mg/g) is calculated according to the pre-harvest treatment when the root and leaf stem weight is mixed at 7:3. Thus, the results are shown in Table 4 below.

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 pre-harvest treatment unprocessed blue light geraniol geraniol + blue light total amount 32.24 35.39 35.39 36.26 Relative amount based on untreated group (%) 100 110 109 112

According to this, when the roots and leaf stems of hydroponic ginseng were mixed in a 7:3 weight ratio, the hydroponic ginseng of Example 2 treated with geraniol + blue light as a pre-harvest treatment showed the highest ginsenoside content.

In summary, when hydroponic ginseng roots are used, geraniol alone treatment is the most effective in increasing the total ginsenoside content, and when hydroponic ginseng leaf stems are used, geraniol + blue light treatment increases the total ginsenoside content. can be said to be the most effective for In addition, it can be seen that when the leaf stem and root of hydroponic ginseng are mixed and used, treatment with geraniol + blue light is the most effective for increasing the total ginsenoside content.

Formulation Example 1: Preparation of tablets

10 mg of whole dry powder of hydroponic ginseng of Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 2: Preparation of capsules

10 mg of ethanol extract of the whole dry powder of hydroponic ginseng of Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 3: Preparation of powdered health functional food

1,000 mg of whole dry powder of hydroponic ginseng of Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Appropriate amount of inorganic mixture

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

potassium phosphate monobasic 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, but the composition is mixed in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, the granules can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 4: Preparation of beverage

1,000 mg of whole dry powder of hydroponic ginseng of Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 ml with purified water

에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2 L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 기능성 음료 조성물 제조에 사용한다. After mixing the above ingredients according to the usual health drink manufacturing method, 85 for about 1 hour

After stirring and heating in a filtration, the resulting solution is obtained in a sterilized 2 L container, sealed and sterilized, and then stored in a refrigerator, and then used in the preparation of the functional beverage composition of the present invention.

Below, a formulation example of a cosmetic composition containing hydroponic ginseng with increased ginsenoside content of the present invention, or a ginsenoside extract extracted therefrom is exemplified. The composition ratio of the formulation example below may be arbitrarily modified according to regional and ethnic preferences such as the demanding class, the demanding country, and the intended use.

Formulation Example 5: Preparation of soap

5 wt% of dry powder of the entire hydroponic ginseng of Example 1

Fat 75% by weight

Sodium hydroxide 5% by weight

perfume 10% by weight

Purified water remaining

A soap is prepared by mixing the above ingredients according to a conventional soap manufacturing method.

Formulation Example 6: Preparation of lotion

3 wt% of dry powder of the entire hydroponic ginseng of Example 1

1 wt% of L-ascorbic acid-2-phosphate magnesium salt

Water-soluble collagen (1% aqueous solution) 1% by weight

0.1 wt% sodium citrate

citric acid 0.05% by weight

Licorice extract 0.2 wt%

1,3-butylene glycol 3 wt%

Purified water remaining

A lotion is prepared by mixing the above ingredients according to a conventional lotion preparation method.

In the above, although embodiments of the present invention have been described, those of ordinary skill in the art can add, change, delete or add components within the scope that does not depart from the spirit of the present invention described in the claims. The present invention may be variously modified and changed by, etc., and this will also be included within the scope of the present invention.

Claims (7)

A method of cultivation of hydroponic ginseng comprising; a pre-harvest treatment step of treating geraniol before harvesting. According to claim 1,
The pre-harvest treatment step is a method of cultivation of hydroponic ginseng, characterized in that the blue light treatment is additionally performed together with the geraniol treatment. According to claim 1,
The blue light treatment is a method of cultivation of hydroponic ginseng, characterized in that it is performed in a bright state in which white light is irradiated in a photoperiod including a light state and a dark state. According to claim 1,
The geraniol (Geraniol) treatment is a cultivation method of hydroponic ginseng, characterized in that performed according to foliar application (foliar application). According to claim 1,
The geraniol treatment is a cultivation method of hydroponic ginseng, characterized in that the geraniol is treated at a concentration of 100 to 1000 ppm. According to claim 1,
The method of cultivation of hydroponic ginseng, characterized in that the geraniol (Geraniol) treatment is performed 6 to 8 days before harvest. Hydroponic ginseng, which is cultivated according to the cultivation method of any one of claims 1 to 6, and characterized in that ginsenosides are increased by 10% or more compared to hydroponic ginseng that is not pre-harvested.

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