KR102457129B1 - Method for cultivating hydroponic-cultured legume for increasing isoflavone content and the use of it - Google Patents

Abstract

The present invention relates to a hydroponics method of legumes comprising a; pre-harvest treatment step of treating Abscisic acid (ABA) before harvesting. Thereby, it is possible to obtain legumes that are grown in the same cultivation method and have a high total isoflavone content of 20% or more compared to legumes that are not pre-harvested.

Description

Hydroponic cultivation method of legumes for increasing the content of isoflavones and the use of legumes prepared therefrom {Method for cultivating hydroponic-cultured legume for increasing isoflavone content and the use of it}

The present invention relates to a hydroponic cultivation method of legumes, to the use of legumes prepared therefrom, and more particularly, to a hydroponic cultivation method of legumes to increase isoflavone content by pre-harvest treatment, and legumes prepared therefrom It is about the use of plants.

Legumes can be divided into a part growing on the ground (above part) and a part growing in the ground (underground part). The above-ground part consists of stems, leaves, and flowers, and the underground part consists of roots, which has a unique root nodule. When a seed germinates and rises to the ground, two cotyledons appear first, followed by a stem that continues to grow. All stems have fine hairs called hairy hairs on the surface of the plant above ground except for petals. Each plant part has its own role and must be balanced to produce a healthy plant and high yield.

If you look at the shape and structure of beans, the part connected to the pod is called the navel, and both sides of the navel have a main hole and a junction, respectively. there is a congruence to The weight of the cotyledon is 85-93% of the cotyledon grain, 5-3% of the skin surrounding the cotyledon, and about 2% of the buds and the young roots of the hypocotyl.

The cotyledon, which occupies most of the seeds, is a source of nutrients while the beans germinate and appear above the ground, and the seed hull plays a role in protecting them from fungi and bacteria in the ground after sowing. So, if the seed coat is damaged before germination, it will not be able to grow into a strong seedling. Also, the young root has a slightly convex shape at the tip of the navel, and can be clearly seen when the seed coat is removed.

The color of the seed coat and the navel of the seed is very diverse, and the color of the seed coat can be largely divided into a single color and a speckled color with stripes or spots. Yellow beans are the most common single color, and there are green beans called cheongtae, brown beans called chestnut beans, and black beans.

Such legumes contain isoflavones, and isoflavones are flavonoid-based substances and have a structure and activity similar to that of estrogen, a female hormone, and are therefore also known as phytoestrogen. In general, the isoflavone content of soybeans is about 0.1 to 0.4%, and the isoflavones contained in soybeans are composed of genistein, daidzein, glycitein and glycosides.

The anticancer effect of isoflavones has been reported in most studies on breast cancer. In addition to breast cancer, it is known to play a positive role in the prevention of prostate cancer, colon cancer, cardiovascular disease, osteoporosis, and menopause syndrome. As such, research results have been reported that isoflavone intake through food containing legumes is beneficial for various health problems.

International Patent Publication WO2017138785A1

It is an object of the present invention to provide a method for growing legumes using Abscisic acid (ABA) treatment, which can improve the content of isoflavones in cultivating legumes.

Another object of the present invention is to provide a food composition, a cosmetic composition, and a feed composition comprising legumes grown according to the method for growing legumes using the Abcisic acid treatment as an active ingredient.

According to one aspect of the invention,

Abscisic acid before harvest (Abscisic acid, ABA) is a harvest pre-treatment step of processing; is provided a cultivation method of legumes comprising a.

The method of growing legumes can increase the content of isoflavones in leaves and stems of legumes.

The cultivation method of the legumes may be performed according to hydroponics.

The legumes are selected from the group consisting of oyster bean, eye-spotted bean, Iksan namul bean, junjeori bean, Eunha bean, Gwangan bean, green bean, silk bean sprout, dawon bean, sowon bean, rat eye bean, iri no. 3 bean, and iri no. 5 bean. It may be any one bean sprout bean.

The abscisic acid treatment may be performed according to foliar application.

The abscisic acid treatment may be performed at a concentration of 100 to 1000 ppm of the abscisic acid.

The abscisic acid treatment may be performed 6 to 8 days before harvest.

According to another aspect of the present invention,

It provides legumes grown according to the above-described cultivation method and having an isoflavone content increased by 20% or more compared to legumes that have not been pre-harvested.

The legumes may further include glycitein in isoflavones as compared to legumes that have not been pre-harvested.

The cultivation method of legumes using abscisic acid treatment of the present invention has the effect of increasing the isoflavone content of the leaf stem by 20% or more compared to the cultivation method of legumes without pre-harvest treatment.

1 is a (2) formally completed photograph of Example 1.
Figure 2 is a photograph of the soybean plant in oryllata 1 to 7 days after planting in the (3) cultivation step of Example 1.
Figure 3 is a photograph of the soybean plant in the algae when 8 to 14 days after the planting of the (3) cultivation step of Example 1.
Figure 4 is a photograph of the soybean plant of oryllata 15 to 21 days after planting of the (3) cultivation step of Example 1.
Figure 5 is a photograph of the soybean plant of Duck altae 22 to 28 days after planting of the (3) cultivation step of Example 1.
Figure 6 is a photograph of the soybean plant of Duck altae after harvesting (5) of Example 1.

Hereinafter, a method for cultivating legumes to increase the isoflavone content of the present invention will be described.

The cultivation method of legumes for increasing the content of isoflavones of the present invention is characterized in that it comprises a step of treating with Abscisic acid (ABA) before harvesting.

In general, the plant cultivation method using nutrient solution is a hydroponics method in which plants are grown using liquid nutrient solution. have.

The cultivation method of legumes is preferably carried out according to hydroponics, and more preferably carried out according to the spray culture method among the methods of hydroponics.

The aeroponic cultivation method is an aeroponics method for cultivating plants using a spray nutrient solution formed using a general sprayer, and a fog phonics method for growing plants using a spray nutrient solution formed using an ultrasonic module ( It can be divided into fogponics method. Since the particles of the nutrient solution used in Fogphonics are smaller than those of Aerophonics, the nutrient solution sprayed in Fogphonics is affected by gravity relatively less than Aerophonics, so it can supply the nutrient solution to the entire planted plant relatively evenly. there are advantages to A fog phonics device, that is, a nutrient solution in a method of spraying a nutrient solution using an ultrasonic vibration module, is generally a liquid nutrient solution stored in a cultivation bed in which plants are planted and grown, and ultrasonic waves provided in the cultivation bed It may be configured in a form in which the liquid nutrient solution is sprayed by the vibration module.

If necessary, the aeroponic cultivation method can be used by selecting an aerophonics method or a phorphonics method, and it is more preferable to use an aerophonic method for the legume cultivation method of the present invention.

The legumes are selected from the group consisting of oyster bean, eye-spotted bean, Iksan namul bean, junjeori bean, Eunha bean, Gwangan bean, green bean, silk bean sprout, dawon bean, sowon bean, rat eye bean, iri no. 3 bean, and iri no. 5 bean. It is preferable that it is any one bean sprout bean. However, the scope of the present invention is not limited thereto, and all legumes that can be grown hydroponically are applicable.

Abscisic acid (ABA) is a compound represented by the following formula (1), and is a plant growth regulator first discovered as a substance inhibiting indole acetic acid in cotton fruits. Dozens of homologues of abscisic acid have been found, and the activity is shown only in those having a specific molecular structure. Many are interconverted within plants. The substance called dolmin, which was dormant in white birch and maple trees, turned out to be abscisic acid. Abcisic acid forms a double layer and causes flowers, leaves, fruits, and annual stems to fall off. It accelerates the aging of leaves and inhibits growth.

[Formula 1]

The abscisic acid treatment is preferably foliar application at a concentration of 100 to 1000 ppm, more preferably 200 to 800 ppm, even more preferably 400 to 600 ppm concentration.

The abscisic acid treatment before harvest is preferably performed 6 to 8 days before harvest, more preferably 6.5 to 7.5 days before, and even more preferably 7 days before harvest.

Before the harvest pre-treatment step, legumes can be grown sequentially according to the steps below.

First, a seedling of legumes is prepared (step a: seedling step).

Next, seedlings of legumes that have passed through the seedling stage are planted (step b: planting stage).

In this formulation step, the pH of the nutrient solution may be 6.3 to 6.7.

In the planting stage, it is preferable that the EC of the nutrient solution is 0.45 to 0.55 dS/m.

In addition, the irrigation cycle is preferably every 5 to 15 minutes for 100 to 140 seconds, more preferably every 8 to 13 minutes for 110 to 130 seconds, even more preferably every 10 minutes for 115 to 125 seconds. can do.

Thereafter, the seedlings that have undergone the formal stage are grown (step c: cultivation stage).

In the early stage of the cultivation stage, it is preferable to set the irrigation cycle under the same conditions as in the planting stage, and then gradually increase the irrigation interval to irrigate every 18 to 20 minutes in the middle of cultivation, and every 28 to 32 minutes in the late cultivation.

The pH of the nutrient solution in the cultivation step is preferably 5.8 to 6.2.

In addition, the EC of the nutrient solution was 0.45 to 0.55 dS/m immediately after planting and early in cultivation, 0.65 to 0.75 dS/m in the middle of cultivation, 0.95 to 1.05 dS/m in the late cultivation, and 1.45 to 1.55 dS/m at the end of cultivation. It is preferable to increase gradually. When such a nutrient solution EC is set, the growth of legumes can be achieved most efficiently.

The photoperiod from planting to harvest is preferably 13 to 18 hours/6 to 10 hours of light/dark, more preferably 15 to 17 hours/7 to 9 hours, even more preferably 15.5 to 16.5 hours Setting it to /7.5~8.5 hours is suitable for increasing the growth efficiency of legumes.

In the present invention, the total content of isoflavones based on the dry weight of the dried leaf stems compared to the legumes grown by the above cultivation method, and treated with abscisic acid before harvest, compared to legumes that have not been pre-harvested with abscisic acid. This can be increased by more than 20%. Preferably it can be increased by 23 to 40%, more preferably by 25 to 30%.

In addition, the legumes are characterized in that the legumes additionally contain or have a higher content of glycitein among isoflavones compared to legumes that are not subjected to the harvest pretreatment.

The present invention provides a method for producing an isoflavone extract, characterized in that the isoflavones are extracted from legumes grown according to the cultivation method for increasing the isoflavone content.

The isoflavone extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. In this case, the type of the organic solvent used or the mixing ratio of water and the organic solvent is not particularly limited.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohol, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol may be used as the lower alcohol. In addition to these organic solvents, polar solvents such as acetic acid, dimethyl-formamide (DMFO), dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, and decane , cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene , benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and non-polar solvents such as tetrahydrofuran (THF) may be used.

The isoflavone extract may be a water extract, an ethanol extract, a methanol extract, or an extract using a mixed solvent of any two or more solvents selected from water, ethanol, and methanol.

The preparation of the water extract can be prepared by extracting legumes with increased isoflavone content by the method of the present invention for 2 to 48 hours with water at 40 to 100 ° C. In order to increase the extraction efficiency, it can be prepared by extraction with water added with one or more enzymes selected from the group consisting of cellulase, biscozyme, alcalase and fexin at 40 to 55° C. for 2 to 24 hours.

The preparation of the ethanol extract or the ethanol aqueous solution extract is 2 to 36 hours at 20 to 60 ° C., preferably 40 to the legumes having an increased isoflavone content by the method of the present invention with ethanol or 35 to 75 vol% of an ethanol aqueous solution. It can be prepared by extraction at ~ 50 °C for 2.5 to 6 hours, and more preferably by extraction with 70% by volume of an ethanol aqueous solution at 45 °C for 3 hours.

As used herein, the term 'extract' also includes a fraction obtained by additionally fractionating the extract. That is, the isoflavone extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by concentrating isoflavones by additionally applying a purification process thereto. In addition, the fraction obtained by passing the extract or fraction through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the isoflavone extract of the present invention.

The present invention provides a food composition comprising legumes grown according to the cultivation method for increasing the content of isoflavones or a food composition comprising an isoflavone extract prepared by the method.

When a legume grown according to the cultivation method for increasing the isoflavone content according to the present invention or an isoflavone extract extracted therefrom is used as an active ingredient additive in a health functional food or general food, the legume according to the present invention or The isoflavone extract may be added as it is or may be used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to each purpose of use, such as prevention, health or treatment.

In general, when preparing food or beverage, the legume or isoflavone extract according to the present invention may be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be less than the above range, and in the present invention, there is no problem in safety in terms of using a natural product or an extract therefrom. It can also be used in an amount beyond the range.

The type of food is not particularly limited, and examples of food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and dairy products including ice cream. , various soups, beverages, teas, drinks, alcoholic beverages, and vitamin complexes, and may include all foods in a conventional sense.

Among the health functional foods according to the present invention, beverage foods may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The above-mentioned natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatine and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate may be about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the functional food according to the present invention.

In addition to the above, the food composition includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may contain a carbonation agent used for In addition, the food composition of the present invention may contain flesh for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not limited, but is generally selected in the range of 0.01 to 0.1 parts by weight relative to 100 parts by weight of the composition of the present invention.

The present invention provides a feed composition comprising legumes grown according to the cultivation method for increasing the content of isoflavones or a feed composition comprising an isoflavone extract prepared by the method. The feed composition may be a feed or feed additive.

The feed composition includes organic acids such as citric acid, humic acid, adipic acid, lactic acid, and malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate), polyphenol, catechin, alpha-tocopherol , rosemary extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, any one or more of natural antioxidants such as phytic acid may be further included.

As an auxiliary component of the feed composition, various auxiliary substances such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal enzymes, and live microorganism preparations are grains, for example, pulverized or crushed wheat, oats, barley, corn and rice; plant protein feeds, such as those based on rape, soybean and sunflower; animal protein feeds such as blood meal, meat meal, bone meal and fish meal; After mixing all the dry ingredients and dry additives consisting of sugar and dairy products, for example, various milk powder and whey powder, a liquid component and a component that becomes liquid after heating, that is, a lipid, for example, an animal product optionally liquefied by heating In addition to the main ingredients such as fats and vegetable fats, it can be used together with substances such as nutritional supplements, digestion and absorption enhancers, growth promoters, and disease prevention agents.

The feed composition may be administered alone to the animal in combination with other feed additives in an edible carrier.

The feed composition can be easily administered as a top dressing or by mixing them directly into the animal feed or separately from the feed, in a separate oral formulation, by injection or transdermally, or in combination with other ingredients. Typically, a single daily dose or divided daily doses may be used as is well known in the art.

When the feed composition is administered separately from the animal feed, the dosage form of the composition can be combined with their non-toxic pharmaceutically acceptable edible carriers to prepare an immediate release or sustained release formulation, as is well known in the art. Such edible carriers may be solid or liquid, for example corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol. When a solid carrier is used, the dosage form of the compound may be a tablet, capsule, powder, lozenge or sugar-containing tablet or top dressing in microdispersible form. When a liquid carrier is used, it may be in the form of a soft gelatin capsule, or a syrup or liquid suspension, emulsion or solution. The dosage form may also contain adjuvants such as preservatives, stabilizers, wetting or emulsifying agents, solution promoters, and the like.

The feed composition may be used by adding to the animal feed by immersion, spraying or mixing.

The feed composition can be applied to a number of animal diets including mammals, poultry and fish. More specifically, the diet includes mammals of commercial importance, such as pigs, cattle, sheep, goats, laboratory rodents (rat, mouse, hamster and gerbil), fur-bearing animals (eg mink and fox), and It can be used on zoo animals (eg monkeys and tailless monkeys), as well as livestock (eg cats and dogs). Commonly commercially important poultry include chickens, turkeys, ducks, geese, pheasants and quails. Commercially raised fish such as trout may also be included.

In the method for compounding animal feed including the feed composition, the composition is mixed with the animal feed in an amount of about 1 g to 100 g per 1 kg of feed on a dry weight basis. In addition, after the feed mixture is thoroughly mixed, a hard viscous granular or granular material is obtained depending on the degree of pulverization of the ingredients. It is supplied as a mash or formed into desired discrete shapes for further processing and packaging. At this time, in order to prevent segregation during storage, it is preferable to add water to the animal feed, followed by a conventional pelletizing, inflating, or extruding process. Excess water can be dried off.

The present invention provides a cosmetic composition comprising a legume grown according to the cultivation method for increasing the content of isoflavones or a cosmetic composition comprising an isoflavone extract prepared by the method.

The cosmetic composition of the present invention may be prepared in the form of a lotion (skin), lotion, essence, cream, pack, or the like.

In the formulation, a legume grown according to the cultivation method for increasing the isoflavone content according to the present invention or an isoflavone extract extracted therefrom is contained in an amount of 0.1% by weight or more, preferably 0.2-50% by weight, more preferably 0.3- 30% by weight, most preferably 0.5 to 10% by weight may be used

The cosmetic composition may further include an appropriate carrier, excipient or diluent commonly used in the manufacture of cosmetics.

The carrier, excipient or diluent includes purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, humectant, thickening agent, antioxidant, viscosity stabilizer, chelating agent , buffers, lower alcohols, and the like. If necessary, whitening agents, moisturizing agents, vitamins, sunscreen agents, perfumes, dyes, and antibacterial agents may be further included.

Hydrogenated vegetable oil, castor oil, cottonseed oil, olive oil, palm oil, jojoba oil, and avocado oil may be used as the oil. As the wax, beeswax, spermaceti, carnauba, candelilla, montan, ceresin, liquid paraffin, and lanolin may be used. can be used As the fatty acid, stearic acid, linoleic acid, linolenic acid, and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyl dodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol, and hexadecanol may be used. As the ester, isopropyl myristate, isopropyl palmitate, and butyl stearate may be used. Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate, sodium N-acyl glutamate; cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, sucrose fatty acid ester, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethanol nonionic surfactants such as monoethanol arnide, polyoxypropylene glycol, polyoxyethylene castor oil, polyoxyethylene lanolin, and the like. Glycerin, 1,3-butylene glycol, and propylene glycol may be used as the desiccant, and ethanol and isopropanol may be used as the lower alcohol. Examples of thickeners include sodium alginate, sodium caseinate, gelatin agar, xanthan gum, starch, cellulose ethers (eg, hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, hydroxypropylmethyl cellulose), polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol and sodium carboxymethyl cellulose; and the like. As antioxidants, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, and ethoxyquin are available. As chelating agents, disodium edetate and ethanehydroxy diphosphate are available. citric acid, sodium citrate, boric acid, borax, and disodium hydrogen phosphate are available as buffers.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

[Example]

Example 1: Cultivation of isoflavone-enhanced legumes

(1) Seedling

The soybeans soaked in water overnight were spread on a sieve lined with cloth, and the soybeans were grown using a spray microscope using tap water.

(2) set meal

Seedlings of high-growth soybeans were selected, and the upper part of the root was wrapped with a sponge for cooking and planted in the bed. At this time, the temperature is 23±2℃, the relative humidity is 70±10%, the nutrient solution pH 6.5±0.2, the nutrient solution EC 0.50±0.05, the irrigation cycle 120sec/10min, the light quantity 140μmol, the photoperiod (light(h)/dark(h) )) was maintained at 16/8 condition. A photograph of a soybean plant of Oraltae which has been completed is shown in FIG. 1 .

(3) cultivation

(1 to 7 days after settling)

While maintaining the same temperature and humidity, nutrient solution pH, nutrient solution EC, light quantity, and photoperiod as at the time of planting, and culturing under the conditions of 120 sec/30 min of irrigation cycle, the growth of the soybean plant was observed, and a photograph is shown in FIG. .

(8 to 14 days after settling)

Cultivated under the same conditions as in the previous cultivation step, except that the nutrient solution pH was set to 0.70 ± 0.05, and the growth of soybean plants was observed, and a photograph is shown in FIG. 3 .

(15 to 21 days after formality)

Cultivated under the same conditions as in the previous cultivation step, except that the nutrient solution pH was set to 1.00±0.05, and the growth of the soybean plant was observed, and a photograph is shown in FIG. 4 .

(22 ~ 28 days after the official ceremony)

Cultivation was carried out under the same conditions as in the previous cultivation step, except that the nutrient solution pH was set to 1.50±0.05, and pruning was performed if necessary. Accordingly, the growth of the soybean plant was observed, and a photograph is shown in FIG. 5 .

(4) Harvest pretreatment

(After 28 days after official release)

7 days before harvest, abscisic acid (ABA) was sprayed on the leaves of soybean plants at a concentration of 500 ppm for 2 days.

(5) Harvest

After one week of pre-harvest treatment, legumes were harvested from the root, and separated into roots and leaf stems. A photograph of the harvested duck altae soybean plant is shown in FIG. 6 .

The indoor temperature, indoor relative humidity, nutrient solution pH, nutrient solution EC (Electrical Conductivity), irrigation cycle, light quantity (white light) and photoperiod conditions are summarized in Table 1 below when cultivating the long algae legumes.

step Room temperature (℃) Indoor Humidity (%) Nutrient pH Nutrient EC (dS/m) irrigation cycle
(seconds/minutes) Light quantity (μmol m ^-2 s ^-1 ) Photoperiod (light/dark)
(h/h) (2) set meal 23±2 70±10 6.5±0.2 0.50±0.05 120/10 140 16/8 (3) Cultivation
(1-7 days) 23±2 70±10 6.5±0.2 0.50±0.05 120/30 140 16/8 (3) Cultivation (8-14 days) 23±2 70±10 6.5±0.2 0.70±0.05 120/30 140 16/8 (3) Cultivation (15-21 days) 23±2 70±10 6.5±0.2 1.00±0.05 120/30 140 16/8 (3) Cultivation (22-28 days) 23±2 70±10 6.5±0.2 1.50±0.05 120/30 140 16/8

Comparative Example 1

(4) Auricularia leguminous plants were grown under the same conditions as in Example 1, except that pre-harvest treatment was not performed.

Comparative Example 2

(4) Auricularia leguminous plants were grown under the same conditions as in Example 1, except that Ethephon treatment was performed instead of abscisic acid (ABA) treatment during harvest pretreatment.

Comparative Example 3

(4) Auricularia leguminous plants were grown under the same conditions as in Example 1, except that ethanol (Ethanol) treatment was performed instead of abscisic acid (ABA) treatment during harvest pretreatment.

Comparative Example 4

(4) During harvest pretreatment, blue light treatment was additionally performed with a light amount of 100 to 120 μmol m ^-2 s ^-1 using a blue LED light source with a wavelength of 450 nm for 6 days with abscisic acid (ABA) treatment. Auricularia leguminous plants were grown under the same conditions as in Example 1.

Comparative Example 5

(4) Example except that the blue light treatment was performed with a light amount of 100 to 120 μmol m ⁻² s ⁻¹ using a blue LED light source having a wavelength of 450 nm for 6 days instead of abscisic acid (ABA) treatment during harvest pretreatment Auricularia leguminous plants were grown under the same conditions as in 1.

* Comparative Example 6

During pre-harvest treatment, instead of abscisic acid (ABA) treatment, geraniol treatment is performed, and with this, blue light with a light amount of 100 to 120 μmol m ^-2 s ^-1 using a blue LED light source having a wavelength of 450 nm for 6 days. The legumes were grown under the same conditions as in Example 1, except that the treatment was additionally performed.

[Experimental example]

Experimental Example 1: Measurement of isoflavone content

In order to compare the isoflavone content of the leaf stems of the legumes grown according to Example 1 and Comparative Examples 1 to 6, respectively, 70% (v /v) 10% by weight based on the weight of the aqueous ethanol solution was mixed, and extracted under reflux at 70° C. for 3 hours to obtain an extract of the leaf stem of a leguminous plant. The thus-obtained A. leguminous plant extract was subjected to high-performance liquid chromatography (HPLC), corresponding to isoflavones, such as daidzin, glycitin, genistin, daidzein, The contents of glycitein and genistein were analyzed, and the results are summarized in Table 2 below.

division Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Example 1 Harvest pretreatment unprocessed Espeon ethanol blue light + ABA blue light ABA Daidzin 0.088 0.151 0.105 0.18 0.314 0.076 Glycitin 0.191 0.082 0.127 0.078 0.093 0.151 Genistin 0.383 0.192 0.233 0.178 0.234 0.238 Daidzein 0.012 0.021 0.018 0.038 0.043 0.019 Glycitein - - - - 0.370 Genistein 0.024 0.04 0.041 0.031 0.034 0.032 Total isoflavone content 0.698 0.486 0.525 0.505 0.718 0.886 Relative amount based on untreated group (%) 100 69.6 75.2 72.3 102.9 127.0

According to this, it was found that the total isoflavone content of the legumes of Example 1 treated with abscisic acid (ABA) alone as pre-harvest treatment increased by 27% compared to the untreated group (Comparative Example 1). In addition, specifically looking at the isoflavone component of Example 1, the other types of isoflavones did not show a significant difference compared to the comparative examples, but the content of glycitein not detected in the comparative examples is very high. Accordingly, it was found that the total isoflavone content was also significantly increased. On the other hand, Comparative Example 2 treated with Esepone as pre-harvest treatment and Comparative Example 3 treated with ethanol showed lower isoflavone content than the untreated group. In addition, in the case of Comparative Example 5, in which the blue light treatment was performed as a pre-harvest treatment, the total isoflavone content was slightly higher than that of the untreated group, and when the abscisic acid (ABA) treatment was performed together with the blue light, the isoflavone content was rather lowered. That is, it can be confirmed that when abscisic acid (ABA) is treated alone, it is effective in improving the isoflavone content, particularly the glycitein content.

Formulation Example 1: Preparation of tablets

10 mg of dry powder of leaf stems of leguminous plants of Example 1

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.

Formulation Example 2: Preparation of capsules

10 mg of ethanol extract of dry powder of leaf stems of leguminous plants of Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

0.2 mg magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.

Formulation Example 3: Preparation of powdered health functional food

1,000 mg of dry powder of leaf stems of leguminous plants of Example 1

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Appropriate amount of inorganic mixture

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, but the composition is mixed in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Then, the granules can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Formulation Example 4: Preparation of beverage

1,000 mg of dry powder of leaf stems of leguminous plants of Example 1

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Total 900 ml with purified water

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Below, the formulation examples of the cosmetic composition containing the isoflavone extract of the present invention increased isoflavone legumes, or isoflavones extracted therefrom are exemplified. The composition ratio of the formulation example below may be arbitrarily modified according to regional and ethnic preferences such as the demanding class, the demanding country, and the intended use.

Formulation Example 5: Preparation of soap

5 wt% of dry powder of leaf stems of leguminous plants of Example 1

Fat 75% by weight

Sodium hydroxide 5% by weight

perfume 10% by weight

Purified water remaining

A soap is prepared by mixing the above ingredients according to a conventional soap manufacturing method.

Formulation Example 6: Preparation of lotion

3 wt% of dry powder of leaf stems of leguminous plants of Example 1

1 wt% of L-ascorbic acid-2-phosphate magnesium salt

Water-soluble collagen (1% aqueous solution) 1% by weight

0.1 wt% sodium citrate

citric acid 0.05% by weight

Licorice extract 0.2 wt%

1,3-butylene glycol 3 wt%

Purified water remaining

A lotion is prepared by mixing the above ingredients according to a conventional lotion preparation method.

In the above, although embodiments of the present invention have been described, those of ordinary skill in the art can add, change, delete or add components within the scope that does not depart from the spirit of the present invention described in the claims. The present invention may be variously modified and changed by, etc., and this will also be included within the scope of the present invention.

Claims (9)

A pre-harvest treatment step of processing Abscisic acid (ABA) before harvest;
The abscisic acid treatment is performed according to foliar application 6 to 8 days before harvest,
It is carried out according to the hydroponics method at a temperature condition of 21 to 25 ° C using white light,
A method of growing legumes, characterized in that it is used as a method of increasing the content of isoflavones in leaves and stems of legumes. delete delete According to claim 1,
The legumes are selected from the group consisting of oyster bean, eye-spotted bean, Iksan namul bean, junjeori bean, Eunha bean, Gwangan bean, green bean, silk bean sprout, dawon bean, sowon bean, rat eye bean, iri no. 3 bean, and iri no. 5 bean. A method of growing legumes, characterized in that any one bean sprouts and beans. delete According to claim 1,
The abscisic acid treatment is a method of growing legumes, characterized in that treating the abscisic acid at a concentration of 100 to 1000 ppm. delete The isoflavone content is 20 weight based on the dry weight of the dried leaf stems grown according to the cultivation method of any one of claims 1, 4, and 6, and compared to legumes that have not been pre-harvest-treated. % increased legumes. 9. The method of claim 8,
The legumes are legumes, characterized in that it further comprises glycitein in isoflavones compared to legumes that are not subjected to the harvest pretreatment.

Images