CN106174043A - A kind of big tall scrawny person is enriched with the technology of isoflavone - Google Patents
A kind of big tall scrawny person is enriched with the technology of isoflavone Download PDFInfo
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- CN106174043A CN106174043A CN201610517392.8A CN201610517392A CN106174043A CN 106174043 A CN106174043 A CN 106174043A CN 201610517392 A CN201610517392 A CN 201610517392A CN 106174043 A CN106174043 A CN 106174043A
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- isoflavone
- tall scrawny
- scrawny person
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- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 235000008696 isoflavones Nutrition 0.000 title claims abstract description 29
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 238000005516 engineering process Methods 0.000 title claims abstract description 15
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 23
- 244000068988 Glycine max Species 0.000 claims abstract description 22
- 230000005855 radiation Effects 0.000 claims abstract description 21
- 230000035784 germination Effects 0.000 claims abstract description 17
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 15
- 229940083618 sodium nitroprusside Drugs 0.000 claims abstract description 15
- 239000007921 spray Substances 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 5
- 239000007864 aqueous solution Substances 0.000 claims abstract description 5
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 5
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 claims abstract description 3
- 238000005507 spraying Methods 0.000 claims abstract description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims description 24
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 claims description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 238000007654 immersion Methods 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229930013032 isoflavonoid Natural products 0.000 description 2
- 150000003817 isoflavonoid derivatives Chemical class 0.000 description 2
- 235000012891 isoflavonoids Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000006552 photochemical reaction Methods 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 150000002515 isoflavone derivatives Chemical class 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000008204 material by function Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229940068041 phytic acid Drugs 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000000467 phytic acid Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000003620 semiochemical Substances 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The present invention relates to a kind of big tall scrawny person and be enriched with the technology of isoflavone, it is characterized in that with the soybean kernel with physiologically active as raw material, by known method aqueous sodium hypochlorite solution, its sterilization is placed in the pure water of 3～5 times amount (v/w), 5h is soaked in 30 DEG C, it is subsequently placed in sprouting machine, germinates at 30 DEG C；Period, by medium wave ultraviolet light (UV B) continuous radiation, (wavelength is 280～320nm, radiation length is 30～50cm, radiant intensity is 20～50 μ W cm‑2) germinated soybean 2～4d, and spraying germination 1～3min with 0～15 μM of abscisic acid (ABA) and 0～3mM sodium nitroprusside (SNP) mixed aqueous solution every 1～4h, spray liquid flow is 50～200mL/min.The technology of the present invention is simple to operate, and in big tall scrawny person, isoflavone content reaches 4000～5500 μ g/g DW.
Description
One, technical field
The present invention relates to a kind of big tall scrawny person and be enriched with the technology of isoflavone, belong to technical field of food production.
Two, background technology
The nutrient substance such as soybean kernel rich in proteins, fat, carbohydrate, and isoflavone, γ-aminobutyric acid etc.
Functional materials, but Isoflavones Content In Soybean Seeds is only 0.2～1.6mg g-1(DW).After soybean kernel germinates, different Huang
The key enzyme of ketone synthesis is activated, and thus isoflavone is accumulated.Isoflavone has estrogenic characteristics, anticancer, reduction to human body
The multiple physiological actions such as cardiovascular disease incidence rate and regulation and control immunne response.Conversion technology is used to can further improve different Huang
Ketone content, to expand its range of application.
In plant, the synthesis of secondary metabolite is the result resisting environment stress.Can be enriched with it has been demonstrated that UV-B coerces
Isoflavone like substance；ABA and NO each may participate in the accumulation coercing lower secondary metabolite as important adverse circumstance signaling molecule.So
And, ABA and NO mediation UV-B coerces the effect of lower Its Isoflavonoid Accumulation and is rarely reported.
Patent (publication number CN103120122A, publication date on 05 29th, 2013) discloses " training of homoisoflavone Semen sojae atricolor
Plant method ", the generation of somatic embryos hybridizing after using tissue culture mode to obtain callus, cultivate isoflavone with this
The soybean varieties that content is higher, the method cumbersome, the production cycle is long, is unfavorable for mass production；Patent is (open
Number CN 103265518A, publication date on 08 28th, 2013) disclose " photochemical reaction synthesis isoflavone ", this mode uses
Photochemical reaction enrichment function material, has the shortcomings such as operation is complicated, cost is high, industrialization production difficulty is high.Paper " germinates big
The photoinduction Effect study of Its Isoflavonoid Accumulation in bean " (Xu Maojun etc. China grain and oil journal, 2003,18 (1): 74-77), " illumination
The regulation that soybean leaves phenylalanine lyases (PAL) gene expression and isoflavone are synthesized " (thank to the spirit tinkling of pieces of jade etc. BULLETIN OF BOTANY Vol.,
2000,17 (5): 443-449), only processing with single light source, enrichment isoflavone rate is relatively low.
The present invention is the mode using environment stress and semiochemicals Combined Treatment in soybean germination, uses intelligence
Change sprouting machine and produce beans bud vegetable, use UV-B continuous radiation, and with abscisic acid (ABA, a kind of plant growth regulator) and nitre
General sodium (SNP, a kind of NO propellant) mixed aqueous solution replaces clear water spray, the efficiently concentrating of stratification isoflavone from soybean.
The technology of the present invention has easy and simple to handle, with short production cycle, the advantage of advantage of lower cost, the beneficially rule of Semen sojae atricolor enrichment isoflavone
Modelling produces, and is the new breakthrough producing functional soy bud series products.
Three, summary of the invention
Technical problem:
It is an object of the invention to provide a kind of big tall scrawny person's production technology rich in isoflavone, be different from tradition enrichment different
The production technology of flavone, it is possible to resolve the problems such as operation is complicated, production cost is high, enrichment cycle length, and obtain a kind of nutritive value
The new function big tall scrawny person of health high, health care is clear and definite, isoflavone content is high.
Technical scheme:
The technology of the present invention is as follows: selecting active beans seed is raw material, by seed full, unbroken, by public affairs
It is sterilized by the method aqueous sodium hypochlorite solution known, is subsequently placed in the pure water of 3～5 times amount (V/W), soaks 5h in 30 DEG C
After, it is placed in sprouting machine 30 DEG C of germinations 2～4d.In germination process, with UV-B continuous radiation (wavelength be 280～
320nm, radiation length are 30～50cm, radiant intensity is 20～50 μ W cm-2), and with abscisic acid (ABA) and sodium nitroprusside
(SNP) the big tall scrawny person in sprouting machine is sprayed by mixed aqueous solution (ABA concentration 0～15 μMs, SNP concentration 0～3mM) every 1～4h
Drenching 1～3min, spray liquid flow is 50～200mL/min, until completing germination process.Above-mentioned big tall scrawny person is enriched with isoflavone
Technology comprise the following steps:
(1) to have great-hearted soybean kernel as raw material, by known method aqueous sodium hypochlorite solution, it is sterilized, so
It is placed in the pure water of 3～5 times amount (V/W), soaks 5h in 30 DEG C；
(2) soybean kernel soaked is placed in sprouting machine the temperature germination 2～4d with 30 DEG C；
(3) in soybean germination, with UV-B continuous radiation (wavelength is 280～320nm, radiation length is 30～50cm,
Radiant intensity is 20～50 μ W cm-2), and with 0～15 μM of ABA and 0～3mM SNP mixed aqueous solution every 1～4h to germination
Big tall scrawny person in machine sprays 1～3min, and spray liquid flow is 50～200mL/min, until completing germination process.
Beneficial effect:
Compared with prior art, a kind of big tall scrawny person of the present invention is enriched with the technology of isoflavone and has the advantage that
(1) in the present invention, soybean germination technical operation is simple, and production cost is low, and the enrichment cycle is short, beneficially industry metaplasia
Produce.
(2) in the present invention, Semen sojae atricolor is after germinateing, and the antinutritional factor content such as phytic acid significantly reduces, and this is more beneficial for human body pair
The absorption of nutrient substance, thus improve soybean function content of material, and catered to the consumption concept that full corn is edible.
(3) under UV-B continuous radiation after ABA and SNP solution spraying processes, in big tall scrawny person, the content of isoflavone reaches
4000～5500 μ g/g DW, can realize the efficiently concentrating of isoflavone.
Four, specific embodiments
Embodiment 1:
To have great-hearted soybean kernel as raw material, by known method aqueous sodium hypochlorite solution, it is sterilized, then
It is placed in the pure water of 3～5 times amount (v/w), after soaking 5h in 30 DEG C, seed is inserted in sprouting machine, germination 4d at 30 DEG C,
(wavelength is 280nm to UV-B continuous radiation, radiation length is 50cm, radiant intensity is 20 μ W cm-2Under), spray liquid uses 15 μMs
ABA solution.In big tall scrawny person, isoflavone content is about 4120 μ g/g DW.
Embodiment 2:
Soybean kernel sterilization, immersion process are with embodiment 1.Seed is inserted in sprouting machine, germination 4d, UV-B at 30 DEG C
(wavelength is 290nm to continuous radiation, radiation length is 45cm, radiant intensity is 25 μ W cm-2Under), spray liquid uses 3mM SNP molten
Liquid.In big tall scrawny person, isoflavone content is about 4580 μ g/g DW.
Embodiment 3:
Soybean kernel sterilization, immersion process are with embodiment 1.Seed is inserted in sprouting machine, germination 4d, UV-B at 30 DEG C
(wavelength is 300nm to continuous radiation, radiation length is 40cm, radiant intensity is 30 μ W cm-2Under) spray liquid use 5 μMs of ABA and
2mM SNP solution.In big tall scrawny person, isoflavone content is about 5150 μ g/g DW.
Embodiment 4:
Soybean kernel sterilization, immersion process are with embodiment 1.Seed is inserted in sprouting machine, germination 4d, UV-B at 30 DEG C
(wavelength is 310nm to continuous radiation, radiation length is 30cm, radiant intensity is 50 μ W cm-2Under), spray liquid uses 10 μMs of ABA
With 1mM SNP solution.In big tall scrawny person, isoflavone content is about 5320 μ g/g DW.
Embodiment 5:
Soybean kernel sterilization, immersion process are with embodiment 1.Seed is inserted in sprouting machine, germination 4d, UV-B at 30 DEG C
(wavelength is 320nm to continuous radiation, radiation length is 35cm, radiant intensity is 40 μ W cm-2Under), spray liquid uses 15 μMs of ABA
With 3mM SNP solution.In big tall scrawny person, isoflavone content is about 5480 μ g/g DW.
Embodiments of the present invention have been described in detail above, but this is only to facilitate the example that understands and lift, should be by
It is considered as limitation of the scope of the invention.Equally, any person of ordinary skill in the field all can be according to the technology of the present invention
Scheme and the description of preferred embodiment thereof, make various possible equivalent and change or replace, but all these change or replacement are all
The scope of the claims of the present invention should be belonged to.
Claims (2)
1. a big tall scrawny person is enriched with the technology of isoflavone, it is characterised in that with there is physiologically active beans seed as raw material,
After liquor natrii hypochloritis soaks, it is placed in the sprouting machine of uv b radiation device and mixes with abscisic acid (ABA) and sodium nitroprusside (SNP)
Heshui solution spraying germinates, and specifically includes following steps:
(1) raw material despecking: selecting full grains, germination percentage soybean kernel high, unbroken is raw material；
(2) sterilize and soak: by known method aqueous sodium hypochlorite solution, it being sterilized, be subsequently placed in 3～5 times amount (v/w)
Pure water in, in 30 DEG C soak 5h；
(3) spray germinates: be placed in sprouting machine by the soybean kernel soaked, and is 280 with wavelength under known germination temperature
～320nm, radiation length be 30～50cm, radiant intensity be 20～50 μ W cm-2Medium wave ultraviolet light (UV-B) continuous radiation
Germinated soybean 2～4d, period is 0～15 μM of ABA and 0～3mM SNP mixed aqueous solution is to big tall scrawny person every 1～4h concentration
Spray 1～3min, spray liquid flow is 50～200mL/min.
The most according to claim 1, a kind of big tall scrawny person being enriched with isoflavone, it is characterised in that different Huang in the big tall scrawny person of gained
Ketone content reaches 4000～5500 μ g/g DW.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106888835A (en) * | 2017-02-17 | 2017-06-27 | 兰州大学 | A kind of method of isoflavone content in quick reduction red clover |
| CN108419680A (en) * | 2018-06-13 | 2018-08-21 | 福建农林大学 | A kind of culture medium being conducive to three-coloured amaranth callus growth and Flavonoids Accumulation |
| CN108456702A (en) * | 2017-02-22 | 2018-08-28 | 陕西省微生物研究所 | The method that flavonoids from phellinus yield is improved in phellinus igniarius mycelium fermentation |
| CN109479432A (en) * | 2017-09-11 | 2019-03-19 | 南京农业大学 | A kind of method of soybean bean sprout enrichment daidzein |
| CN109479977A (en) * | 2018-11-16 | 2019-03-19 | 济南大学 | A kind of soymilk and preparation method thereof rich in aglycone isoflavone |
| CN109743926A (en) * | 2018-12-27 | 2019-05-14 | 长沙联美生物科技有限责任公司 | A kind of broccoli sprouting seeding cultivating method of green enrichment general flavone |
| CN110122104A (en) * | 2018-02-02 | 2019-08-16 | 南京农业大学 | A kind of high gamma-aminobutyric acid and low phytic acid soybean bean sprout and its production method |
| CN110122295A (en) * | 2018-02-02 | 2019-08-16 | 南京农业大学 | A kind of low phytic acid Vigna beans bean sprout and its production method |
| CN111990188A (en) * | 2020-08-21 | 2020-11-27 | 扬州大学 | Production method for enriching content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouts and improving yield |
| CN112154728A (en) * | 2020-08-20 | 2021-01-01 | 扬州大学 | Soybean powder rich in soybean isoflavone and its preparation method and application |
| KR20220108382A (en) * | 2021-01-27 | 2022-08-03 | 주식회사 우리기술 | Method for cultivating hydroponic-cultured legume for increasing isoflavone content and the use of it |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106888835A (en) * | 2017-02-17 | 2017-06-27 | 兰州大学 | A kind of method of isoflavone content in quick reduction red clover |
| CN108456702B (en) * | 2017-02-22 | 2021-08-24 | 陕西省微生物研究所 | Method for improving yield of phellinus igniarius flavone in phellinus igniarius mycelium fermentation |
| CN108456702A (en) * | 2017-02-22 | 2018-08-28 | 陕西省微生物研究所 | The method that flavonoids from phellinus yield is improved in phellinus igniarius mycelium fermentation |
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