CN110122295A - A kind of low phytic acid Vigna beans bean sprout and its production method - Google Patents
A kind of low phytic acid Vigna beans bean sprout and its production method Download PDFInfo
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- CN110122295A CN110122295A CN201810125342.4A CN201810125342A CN110122295A CN 110122295 A CN110122295 A CN 110122295A CN 201810125342 A CN201810125342 A CN 201810125342A CN 110122295 A CN110122295 A CN 110122295A
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- vigna
- phytic acid
- seed
- bean sprout
- beans
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B15/00—Organic phosphatic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The present invention relates to a kind of low phytic acid Vigna beans bean sprout and its production methods, belong to technical field of food production.It is characterized in that after screening, hypochlorite disinfectant, clear water impregnates using the Vigna beans seed with physiological activity as raw material, it is subsequently placed in sprouting machine, is protected from light germination；Period uses 5~50 μm of ol/L hydrogen peroxide (H2O2) and 50~1000 μm of ol/L sodium nitroprussiate (SNP) mixed aqueous solution spray germinations.Simple, at low cost, with short production cycle, the suitable industrialized production of operation of the present invention, Vigna bean sprout phytic acid degradation rate obtained reach 45~85%.
Description
One, technical field
The present invention relates to a kind of low phytic acid Vigna beans bean sprout and its production methods, belong to technical field of food production.
Two, background technique
Mung bean, red bean and cowpea be pulse family cowpea platymiscium, seed it is full of nutrition and have healthcare function, China
There are more than 2000 years cultivation histories.However the presence of phytic acid reduces nutritive value in its seed.Phytic acid be in plant phosphorus and
The major storage form of inositol, while there is extremely strong sequestering power, it is easy to the minerals member such as protein and calcium, zinc, potassium, magnesium, iron
Element chelating, forms insoluble complex, influences the absorption of people and nonruminant to these protein and mineral element.Using biology
Transformation technology can be effectively reduced phytic acid content, improve food value.
When Vigna beans seed is sprouted the metabolism that is carried out and energetic supersession all with phosphorus-containing matter (such as DNA,
RNA, ATP and the lecithin etc. for constituting cell membrane) it is closely related.When Vigna beans seed is sprouted, endogenous phytase is activated,
Phytic acid decomposes under phytase and generates inositol and rudimentary inositol monophosphate (IP1-IP5), and release the albumen being chelated
Matter, phosphoric acid and mineral element.Phosphoric acid participates in the metabolism of plant energy i (in vivo), and inositol participates in the forming process of cell wall, thus right
Vigna beans seed is sprouted and growth of seedling is extremely important.It is adjusted it has been demonstrated that adding external source during beans Grain germination
Agent can effectively and selectively reduce phytic acid content.H2O2It is all there is bioactivity and Role in Plant Signal Transduction easy with NO
Diffusing molecules to the growth of plant seed germination, root and blade, resist adverse circumstance etc. with direct physiological regulation function.
H2O2With both NO interaction, influence each other, the development and metabolism of synergic adjustment plant cell.External source H2O2With NO to cowpea
The influence for belonging to phytic acid degradation in bean sprout production process is rarely reported, present invention external source H2O2Joint SNP (NO donor) sprays cowpea
Belong to beans seed, is remarkably improved phytase activity in Vigna bean sprout, efficient degradation phytic acid.
" Vicia faba seedling oxidation resistance is to exogenous NO gas and H under Cd stress for paper2O2Response " (the Acta Prataculture such as Li Yuan,
2009,18 (6): 186-191) use earth culture method Study of Exogenous NO and H2O2To Vicia faba seedling oxidation resistance during Cd stress
It influences, is not directed to the influence degraded to phytic acid；Patent (publication number CN101084732, publication date on December 12nd, 2007) is open
" a kind of production method of fast-growing mung bean sprouts ", the method eluriated using hot-water soak, cold water, it is raw to educate bud, water drenching vernalization mode
Mung bean bean sprout is produced, this method step is many and diverse, and is not directed to the degradation to phytic acid.Patent (publication number CN101011026, publication date
08 month 2007 No. 08) disclose " a kind of method of quickly breeding low phytic acid crop ", using Mutation induction technology, the low plant of breeding
Sour crop, the operation difficulty is big, and the production cycle is long, is unfavorable for factory mass production；Patent (publication number CN103750175A,
Publication date 04 month 2014 30) disclose " a kind of low phytic acid beans bean sprout production technology ", the method using citric acid and
CaCl2Mixing spray Germination methods reduce soybean bean sprout phytic acid content, but used in the method immersion medium, culture solution group
Point and phytic acid degradation rate there are significant differences with the present invention.
The present invention produces Vigna bean sprout using intelligent sprouting machine, uses H2O2It is sprayed with SNP mixed aqueous solution, promotes cowpea
The efficient degradation of phytic acid in Macroptilium bean sprout, the production for low phytic acid Vigna bean sprout provide new technology.
Three, summary of the invention
Technical problem:
The present invention is intended to provide a kind of low phytic acid Vigna beans bean sprout production method, the cowpea for avoiding the prior art from producing
Belong to the high problem of bean sprout phytic acid content.This method is simple and easy to do, and the phytic acid degradation rate of Vigna bean sprout can be improved in a short time.
Technical solution:
The technology of the present invention is as follows: using the Vigna beans seed with physiological activity as raw material, by full grains, germination percentage
After the aqueous sodium hypochlorite solution disinfection treatment that high, unbroken seed volume ratio is 1%, being placed in water and quality grain ratio is 1:
In 2.5~4.5 clear water, after 30 DEG C of 5~6h of immersion, places it in sprouting machine, be protected from light 2~4d of germination at 30 DEG C.Germination
It in the process, is 5~50 μm of ol/L H with concentration2O2It is spray liquid with 50~1000 μm of ol/L SNP mixed aqueous solutions, to cowpea
Belonging to bean sprout and sprays 1~3min every 1~2h, spray flow quantity is 50~200mL/min, per culture solution of replacement for 24 hours, until
Complete the germination process of 2~4d.
In the method for above-mentioned Vigna bean sprout degradation phytic acid, preferred method is:
(1) using the Vigna beans seed with physiological activity as raw material, the aqueous sodium hypochlorite solution for being 1% with volume ratio
After disinfection treatment, it is placed in the clear water that water and quality grain ratio are 1: 4, in 30 DEG C of immersion 5h；
(2) seed after immersion is placed in sprouting machine and is protected from light germination 4d with 30 DEG C；
(3) in germination process, with 25 μm of ol/L H2O2With 500 μm of ol/L SNP mixed aqueous solutions to the cowpea in sprouting machine
Belong to bean sprout and spray 2min every 1h, spray flow quantity is 150mL/min, and per a culture solution is replaced for 24 hours, germinate 4d, terminates hair
Bud process.Vigna bean sprout phytic acid degradation rate obtained is 85%.
The utility model has the advantages that
Compared with existing germination technology, the invention has the following advantages that
(1) H used in2O2With SNP mixed aqueous solution can activated plant endogenous enzymes, it is horizontal to improve the physiological metabolism of Vigna bean sprout,
Promote the growth of Vigna bean sprout, shorten the germination period, yield is high, it is tender and crisp spend, good quality；
(2) through H2O2With SNP mixed aqueous solution spray Vigna bean sprout, it can be achieved that phytic acid efficient degradation, phytic acid degradation
Rate is up to 45~85%；
(3) rudimentary inositol phosphorus more with physiological function are generated with the raising of phytic acid degradation rate, in Vigna bean sprout
Acid, and the protein and mineral element being more chelated are discharged, improve Vigna bean sprout nutritional quality.
Four, specific embodiment
Embodiment 1:
Select full grains, germination percentage height, unbroken mung bean seed, the secondary chlorine for being 1% by known method volume ratio
After sour sodium disinfection treatment, it is placed in the clear water that water and mung bean mass ratio are 1: 4, in 30 DEG C of immersion 5h；By the mung bean seed after immersion
In grain merging sprouting machine, it is protected from light germination 2d at 30 DEG C, during which, uses 5 μm of ol/L H every 1h2O2It is mixed with 50 μm of ol/L SNP
Heshui solution spraying 2min, spray flow quantity are 150 mL/min, per culture solution of replacement for 24 hours.Phytic acid drops in mung bean bean sprout
Solution rate is 45%.
Embodiment 2:
The selection of red bean seed, disinfection, immersion process are the same as embodiment 1.Red bean seed after immersion is placed in sprouting machine,
It is protected from light germination 3d at 30 DEG C, during which, uses 20 μm of ol/L H every 1h2O2It is sprayed with 600 μm of ol/L SNP mixed aqueous solutions
2min, spray flow quantity is with embodiment 1, per culture solution of replacement for 24 hours.Red bean bean sprout phytic acid degradation rate obtained is 65%.
Embodiment 3:
The selection of mung bean seed and sterilization method are the same as embodiment 1.Mung bean seed after disinfection is placed in water and mung bean mass ratio
For in 1: 3 clear water, in 30 DEG C of immersion 6h；By in the mung bean seed merging sprouting machine after immersion, germination is protected from light at 30 DEG C
During which 3d uses 10 μm of ol/L H every 1h2O23min is sprayed with 200 μm of ol/L SNP mixed aqueous solutions, spray flow quantity is
200mL/min, per culture solution of replacement for 24 hours.Mung bean bean sprout phytic acid degradation rate obtained is 70%.
Embodiment 4:
The selection of cowpea seed and sterilization method are the same as example 1.Cowpea seed after disinfection is placed in water and cowpea mass ratio is 1
: in 4.5 clear water, in 30 DEG C of immersion 5.5h；By in the cowpea seed merging sprouting machine after immersion, germinate 4d at 30 DEG C, the phase
Between, 30 μm of ol/L H are used every 1h2O22min is sprayed with 800 μm of ol/L SNP mixed aqueous solutions, sprays the same embodiment of flow quantity
1, per culture solution of replacement for 24 hours.Cowpea bean sprout phytic acid degradation rate obtained is 80%.
Have been described in detail above embodiments of the present invention, but this is only to facilitate the example for understanding and lifting, it should not be by
It is considered as and limits the scope of the present invention.Equally, any person of ordinary skill in the field can technology according to the present invention
Various possible equivalent changes or replacement are made in the description of scheme and its preferred embodiment, but all these changes or replacement are all
It should belong to scope of protection of the claims of the invention.
Claims (2)
1. a kind of low phytic acid Vigna beans bean sprout production method, which is characterized in that with the Vigna beans with physiological activity
Seed is raw material, impregnates, is subsequently placed in sprouting machine with hydrogen peroxide (H through screening, hypochlorite disinfectant, clear water2O2) and nitre it is general
The spray germination of sodium (SNP) mixed aqueous solution, specifically includes the following steps:
(1) raw material despecking: selecting full grains, germination percentage height, unbroken Vigna beans seed is raw material；
(2) disinfection and immersion: well known method is pressed, the aqueous sodium hypochlorite solution for being 1% with volume ratio is to Vigna beans seed
It carries out disinfection, is subsequently placed in the clear water that water and Vigna beans quality grain ratio are 1: 2.5~4.5 and impregnates, soaking temperature 30
DEG C, 5~6h of soaking time；
(3) spray germination: the Vigna beans seed after immersion is placed in sprouting machine, at 30 DEG C of germination temperature, every 1~
2h is 5~50 μm of ol/L H using concentration2O2With 50~1000 μm of ol/LSNP mixed aqueous solutions to Vigna beans seed spray 1
~3min, spray flow quantity are 50~200mL/min, per a culture solution is replaced for 24 hours, spray 2~4d of germination.
2. a kind of according to claim 1, low phytic acid Vigna beans bean sprout production method, it is characterised in that: raw material is green
Beans, red bean and cowpea seed, phytic acid degradation rate reaches 45~85% in Vigna bean sprout obtained.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810125342.4A CN110122295B (en) | 2018-02-02 | 2018-02-02 | Low phytic acid cowpea bean sprouts and production method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810125342.4A CN110122295B (en) | 2018-02-02 | 2018-02-02 | Low phytic acid cowpea bean sprouts and production method thereof |
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| CN110122295A true CN110122295A (en) | 2019-08-16 |
| CN110122295B CN110122295B (en) | 2021-09-24 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4519163A (en) * | 1982-09-22 | 1985-05-28 | Phytogen | Controlled growth of mung bean sprouts |
| CN103750175A (en) * | 2014-01-14 | 2014-04-30 | 南京农业大学 | Production technology of low-phytic acid bean sprouts |
| CN106174043A (en) * | 2016-06-29 | 2016-12-07 | 南京农业大学 | A kind of big tall scrawny person is enriched with the technology of isoflavone |
| CN106922509A (en) * | 2017-03-16 | 2017-07-07 | 扬州大学 | It is a kind of to be enriched with tall scrawny person gamma aminobutyric acid content and improve the production method of yield |
-
2018
- 2018-02-02 CN CN201810125342.4A patent/CN110122295B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4519163A (en) * | 1982-09-22 | 1985-05-28 | Phytogen | Controlled growth of mung bean sprouts |
| CN103750175A (en) * | 2014-01-14 | 2014-04-30 | 南京农业大学 | Production technology of low-phytic acid bean sprouts |
| CN106174043A (en) * | 2016-06-29 | 2016-12-07 | 南京农业大学 | A kind of big tall scrawny person is enriched with the technology of isoflavone |
| CN106922509A (en) * | 2017-03-16 | 2017-07-07 | 扬州大学 | It is a kind of to be enriched with tall scrawny person gamma aminobutyric acid content and improve the production method of yield |
Non-Patent Citations (1)
| Title |
|---|
| 王三根 主编: "《高级植物生理教程》", 31 December 2010 * |
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