CN105695503A - Seed breeding method of wheat - Google Patents
Seed breeding method of wheat Download PDFInfo
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- CN105695503A CN105695503A CN201610173930.6A CN201610173930A CN105695503A CN 105695503 A CN105695503 A CN 105695503A CN 201610173930 A CN201610173930 A CN 201610173930A CN 105695503 A CN105695503 A CN 105695503A
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- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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Abstract
The invention discloses a seed breeding method of wheat. The quality character of a variety is greatly guaranteed through selecting and using a good variety; saline-alkaline water is irrigated during several growth and development critical periods of the wheat; a salt-alkali-resistant wheat plant is picked out; therefore, a salt-alkali-resistant variety which is stable in character is obtained through continuous selfing; afterwards, a DNA (Deoxyribonucleic Acid) plasmid containing a pinellia pedatisecta agglutinin gene and a coding gene of OsMADS57 protein is introduced into target wheat through a gene gun method; on one hand, anti-aphid transgenic wheat is obtained, and on the other hand, a plant height, wheatear and grain numbers and a grain weight are increased appropriately; tillers are reduced appropriately; the lodging-resistant ability is increased; therefore, the yield of a colony is enabled to reach a maximum.
Description
Technical field
The present invention relates to field of wheat breeding, be specifically related to a kind of method for breeding wheat。
Background technology
Semen Tritici aestivi is the general designation of Semen Tritici aestivi system plant, is a kind of grass in extensively plantation all over the world, and the caryopsis of Semen Tritici aestivi is one of staple food of the mankind, can make the foods such as bread, steamed bread, cookies, noodles after wearing into flour；Medicated beer, ethanol, vodka or raw matter fuel is can be made into after fermentation。Semen Tritici aestivi rich in starch, protein, fat, mineral, calcium, ferrum, thiamine, riboflavin, nicotinic acid, vitamin A and vitamin C etc.。Semen Tritici aestivi is a big chunk that the cereal crops that China is important, yield and consumption figure account for whole nation grain aggregate, is the staple food of northern China resident, occupies very important status in agricultural production and national economy。The national conditions having a large population and a few land due to China and the lasting minimizing in soil, Semen Tritici aestivi imbalance between supply and demand is more and more prominent。
Summary of the invention
For solving the problems referred to above, the invention provides a kind of method for breeding wheat, DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen imports in purpose Semen Tritici aestivi, obtain the transgenic wheat of anti-aphid on the one hand, suitably add plant height, grain number per spike and grain weight on the other hand, suitably decrease tiller, add capacity for the resistance to lodging, so that population yield has reached maximum, simultaneously institute or must kind there is excellent Saline alkali tolerance。
For achieving the above object, the technical scheme that the present invention takes is:
A kind of method for breeding wheat, comprises the steps:
S1, selection suitable locality plantation, Resistant, the high-quality that anti-fall, tiller is weak, fringe is big, setting percentage is high, high-yield variety are hybridized as Semen Tritici aestivi male parent and Semen Tritici aestivi female parent, obtain cross combination F1 generation seed；
S2, under saline and alkaline planting conditions, utilize gained F1 generation seed selfing formed second cycle line colony, and therefrom select there is Salt And Alkali Tolerance character wheat plant selfing 10 generation more than, obtain the Salt And Alkali Tolerance wheat hybridizing kind that character is stable；
S3, by particle bombardment, the DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported the Seedling cell of above-mentioned Salt And Alkali Tolerance wheat hybridizing kind, tissue or organ, after 75% ethanol surface sterilization, it is placed on division culture medium, shading renewal cultivation one day under 25 DEG C～27 DEG C conditions, after cultivating 3 days under 3000Lx～4000Lx intensity of illumination, it is transferred in 1/2MS+BA4.8mg/L+NAA0.04mg/L culture medium and continues to cultivate；
S4, grow to more than 1cm to resistant buds after, move it into root media: improvement MS+0.04mg/LNAA+0.75mg/L6-BA+0.075mg/LIBA+35g/L sucrose medium continues to cultivate, quality trait is measured by growth of seedling to well developed root system, stem and leaf stalwartness comprehensively, screening yield is high, comprehensive resistance strain strong, colory moves in greenhouse, conventional cultivation, until producing seeds。
Preferably, described saline and alkaline planting conditions includes: before sowing F1 generation seed, Semen Tritici aestivi period of seedling establishment and wheat during jointing stage, watering saline-alkali water respectively in wheat planting district and to saline-alkali water 1～5cm above ground level and keep certain time, every liter of saline-alkali water comprises 26.276 grams of sodium chloride, 2.16 grams of magnesium chloride, 3.148 grams of magnesium sulfate, 1.243 grams of calcium chloride, sodium bicarbonate 0.179 gram, 0.723 gram of potassium chloride。
Preferably, improvement MS in institute step S4 include manganese methionine 10mg/L, zinc sulfate monohydrate 5mg/L, nucleoside hydrochlorate 17mg/L, magnesium amino acid chelate 5mg/L, Selenomethionine 5.5mg/L, phytase 0.75mg/L, medium trace element 4.5mg/L, pyridine acid chromium salt 0.065mg/L, calcium iodate 0.3mg/L, lysine 6mg/L, chelating amino acids cobalt 0.065mg/L, albumen germanium 0.75mg/L, iodine yeast 6mg/L, thiamine hydrochloride 10mg/L, pyridoxine hydrochloride 3mg/L, inositol 35mg/L, cyclohexanhexanol 27mg/L。
Preferably, 5 '-adenylic acid dipotassium that described nucleoside hydrochlorate obtains after including ribonucleic acid basic hydrolysis or sodium, 5 '-bird adenylic acid dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element includes Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2
Preferably, described Salt And Alkali Tolerance character includes any three kinds in following character:
Wheat seed: long awns, white shell and white grain；
Plant height: 65～85cm；
Fruit ear: grain number per spike 35～45, mass of 1000 kernel 45～55g, fecundity is good；Yield: 485～545kg/ mu。
The method have the advantages that
DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported in purpose Semen Tritici aestivi by particle bombardment by the present invention, obtain the transgenic wheat of anti-aphid on the one hand, suitably add plant height, grain number per spike and grain weight on the other hand, suitably decrease tiller, add capacity for the resistance to lodging, so that population yield has reached maximum, simultaneously institute or must kind there is excellent Saline alkali tolerance。
Detailed description of the invention
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated。Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention。
The standard that in following example, Salt And Alkali Tolerance character judges includes any three kinds in following character: wheat seed: long awns, white shell and white grain；Plant height: 65～85cm；Fruit ear: grain number per spike 35～45, mass of 1000 kernel 45～55g, fecundity is good；Yield: 485～545kg/ mu。
Embodiment 1
S1, selection suitable locality plantation, Resistant, the high-quality that anti-fall, tiller is weak, fringe is big, setting percentage is high, high-yield variety are hybridized as Semen Tritici aestivi male parent and Semen Tritici aestivi female parent, obtain cross combination F1 generation seed；
S2, under saline and alkaline planting conditions, utilize gained F1 generation seed selfing formed second cycle line colony, and therefrom select there is Salt And Alkali Tolerance character wheat plant selfing 10 generation more than, obtain the Salt And Alkali Tolerance wheat hybridizing kind that character is stable；Described saline and alkaline planting conditions includes: before sowing F1 generation seed, Semen Tritici aestivi period of seedling establishment and wheat during jointing stage, watering saline-alkali water respectively in wheat planting district and to saline-alkali water 1cm above ground level and keep certain time, every liter of saline-alkali water comprises 26.276 grams of sodium chloride, 2.16 grams of magnesium chloride, 3.148 grams of magnesium sulfate, 1.243 grams of calcium chloride, sodium bicarbonate 0.179 gram, 0.723 gram of potassium chloride。
S3, by particle bombardment, the DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported the Seedling cell of above-mentioned Salt And Alkali Tolerance wheat hybridizing kind, tissue or organ, after 75% ethanol surface sterilization, it is placed on division culture medium, shading renewal cultivation one day under 25 DEG C of conditions, after cultivating 3 days under 3000Lx～4000Lx intensity of illumination, it is transferred in 1/2MS+BA4.8mg/L+NAA0.04mg/L culture medium and continues to cultivate；
S4, grow to more than 1cm to resistant buds after, move it into root media: improvement MS+0.04mg/LNAA+0.75mg/L6-BA+0.075mg/LIBA+35g/L sucrose medium continues to cultivate, quality trait is measured by growth of seedling to well developed root system, stem and leaf stalwartness comprehensively, screening yield is high, comprehensive resistance strain strong, colory moves in greenhouse, conventional cultivation, until producing seeds。Described improvement MS include manganese methionine 10mg/L, zinc sulfate monohydrate 5mg/L, nucleoside hydrochlorate 17mg/L, magnesium amino acid chelate 5mg/L, Selenomethionine 5.5mg/L, phytase 0.75mg/L, medium trace element 4.5mg/L, pyridine acid chromium salt 0.065mg/L, calcium iodate 0.3mg/L, lysine 6mg/L, chelating amino acids cobalt 0.065mg/L, albumen germanium 0.75mg/L, iodine yeast 6mg/L, thiamine hydrochloride 10mg/L, pyridoxine hydrochloride 3mg/L, inositol 35mg/L, cyclohexanhexanol 27mg/L。5 '-adenylic acid dipotassium that described nucleoside hydrochlorate obtains after including ribonucleic acid basic hydrolysis or sodium, 5 '-bird adenylic acid dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element includes Mn, B, Fe, Zn, Cu and Mo, described Mn B: Fe: Zn: Cu: Mo weight ratio be 3: 1: 2: 1: 2: 2。
Embodiment 2
S1, selection suitable locality plantation, Resistant, the high-quality that anti-fall, tiller is weak, fringe is big, setting percentage is high, high-yield variety are hybridized as Semen Tritici aestivi male parent and Semen Tritici aestivi female parent, obtain cross combination F1 generation seed；
S2, under saline and alkaline planting conditions, utilize gained F1 generation seed selfing formed second cycle line colony, and therefrom select there is Salt And Alkali Tolerance character wheat plant selfing 10 generation more than, obtain the Salt And Alkali Tolerance wheat hybridizing kind that character is stable；Described saline and alkaline planting conditions includes: before sowing F1 generation seed, Semen Tritici aestivi period of seedling establishment and wheat during jointing stage, watering saline-alkali water respectively in wheat planting district and to saline-alkali water 5cm above ground level and keep certain time, every liter of saline-alkali water comprises 26.276 grams of sodium chloride, 2.16 grams of magnesium chloride, 3.148 grams of magnesium sulfate, 1.243 grams of calcium chloride, sodium bicarbonate 0.179 gram, 0.723 gram of potassium chloride。
S3, by particle bombardment, the DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported the Seedling cell of above-mentioned Salt And Alkali Tolerance wheat hybridizing kind, tissue or organ, after 75% ethanol surface sterilization, it is placed on division culture medium, shading renewal cultivation one day under 27 DEG C of conditions, after cultivating 3 days under 3000Lx～4000Lx intensity of illumination, it is transferred in 1/2MS+BA4.8mg/L+NAA0.04mg/L culture medium and continues to cultivate；
S4, grow to more than 1cm to resistant buds after, move it into root media: improvement MS+0.04mg/LNAA+0.75mg/L6-BA+0.075mg/LIBA+35g/L sucrose medium continues to cultivate, quality trait is measured by growth of seedling to well developed root system, stem and leaf stalwartness comprehensively, screening yield is high, comprehensive resistance strain strong, colory moves in greenhouse, conventional cultivation, until producing seeds。Described improvement MS include manganese methionine 10mg/L, zinc sulfate monohydrate 5mg/L, nucleoside hydrochlorate 17mg/L, magnesium amino acid chelate 5mg/L, Selenomethionine 5.5mg/L, phytase 0.75mg/L, medium trace element 4.5mg/L, pyridine acid chromium salt 0.065mg/L, calcium iodate 0.3mg/L, lysine 6mg/L, chelating amino acids cobalt 0.065mg/L, albumen germanium 0.75mg/L, iodine yeast 6mg/L, thiamine hydrochloride 10mg/L, pyridoxine hydrochloride 3mg/L, inositol 35mg/L, cyclohexanhexanol 27mg/L。5 '-adenylic acid dipotassium that described nucleoside hydrochlorate obtains after including ribonucleic acid basic hydrolysis or sodium, 5 '-bird adenylic acid dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element includes Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2。
Embodiment 3
S1, selection suitable locality plantation, Resistant, the high-quality that anti-fall, tiller is weak, fringe is big, setting percentage is high, high-yield variety are hybridized as Semen Tritici aestivi male parent and Semen Tritici aestivi female parent, obtain cross combination F1 generation seed；
S2, under saline and alkaline planting conditions, utilize gained F1 generation seed selfing formed second cycle line colony, and therefrom select there is Salt And Alkali Tolerance character wheat plant selfing 10 generation more than, obtain the Salt And Alkali Tolerance wheat hybridizing kind that character is stable；Described saline and alkaline planting conditions includes: before sowing F1 generation seed, Semen Tritici aestivi period of seedling establishment and wheat during jointing stage, watering saline-alkali water respectively in wheat planting district and to saline-alkali water 3cm above ground level and keep certain time, every liter of saline-alkali water comprises 26.276 grams of sodium chloride, 2.16 grams of magnesium chloride, 3.148 grams of magnesium sulfate, 1.243 grams of calcium chloride, sodium bicarbonate 0.179 gram, 0.723 gram of potassium chloride。
S3, by particle bombardment, the DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported the Seedling cell of above-mentioned Salt And Alkali Tolerance wheat hybridizing kind, tissue or organ, after 75% ethanol surface sterilization, it is placed on division culture medium, shading renewal cultivation one day under 26 DEG C of conditions, after cultivating 3 days under 3000Lx～4000Lx intensity of illumination, it is transferred in 1/2MS+BA4.8mg/L+NAA0.04mg/L culture medium and continues to cultivate；
S4, grow to more than 1cm to resistant buds after, move it into root media: improvement MS+0.04mg/LNAA+0.75mg/L6-BA+0.075mg/LIBA+35g/L sucrose medium continues to cultivate, quality trait is measured by growth of seedling to well developed root system, stem and leaf stalwartness comprehensively, screening yield is high, comprehensive resistance strain strong, colory moves in greenhouse, conventional cultivation, until producing seeds。Described improvement MS include manganese methionine 10mg/L, zinc sulfate monohydrate 5mg/L, nucleoside hydrochlorate 17mg/L, magnesium amino acid chelate 5mg/L, Selenomethionine 5.5mg/L, phytase 0.75mg/L, medium trace element 4.5mg/L, pyridine acid chromium salt 0.065mg/L, calcium iodate 0.3mg/L, lysine 6mg/L, chelating amino acids cobalt 0.065mg/L, albumen germanium 0.75mg/L, iodine yeast 6mg/L, thiamine hydrochloride 10mg/L, pyridoxine hydrochloride 3mg/L, inositol 35mg/L, cyclohexanhexanol 27mg/L。5 '-adenylic acid dipotassium that described nucleoside hydrochlorate obtains after including ribonucleic acid basic hydrolysis or sodium, 5 '-bird adenylic acid dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element includes Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2。
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention。
Claims (5)
1. a method for breeding wheat, it is characterised in that comprise the steps:
S1, selection suitable locality plantation, Resistant, the high-quality that anti-fall, tiller is weak, fringe is big, setting percentage is high, high-yield variety are hybridized as Semen Tritici aestivi male parent and Semen Tritici aestivi female parent, obtain cross combination F1 generation seed；
S2, under saline and alkaline planting conditions, utilize gained F1 generation seed selfing formed second cycle line colony, and therefrom select there is Salt And Alkali Tolerance character wheat plant selfing 10 generation more than, obtain the Salt And Alkali Tolerance wheat hybridizing kind that character is stable；
S3, by particle bombardment, the DNA plasmid containing Rhizoma pinelliae pedatisectae agglutinin gene and the encoding gene of OsMADS57 albumen is imported the Seedling cell of above-mentioned Salt And Alkali Tolerance wheat hybridizing kind, tissue or organ, after 75% ethanol surface sterilization, it is placed on division culture medium, shading renewal cultivation one day under 25 DEG C～27 DEG C conditions, after cultivating 3 days under 3000Lx～4000Lx intensity of illumination, it is transferred in 1/2MS+BA4.8mg/L+NAA0.04mg/L culture medium and continues to cultivate；
S4, grow to more than 1cm to resistant buds after, move it into root media: improvement MS+0.04mg/LNAA+0.75mg/L6-BA+0.075mg/LIBA+35g/L sucrose medium continues to cultivate, quality trait is measured by growth of seedling to well developed root system, stem and leaf stalwartness comprehensively, screening yield is high, comprehensive resistance strain strong, colory moves in greenhouse, conventional cultivation, until producing seeds。
2. a kind of method for breeding wheat according to claim 1, it is characterized in that, described saline and alkaline planting conditions includes: before sowing F1 generation seed, Semen Tritici aestivi period of seedling establishment and wheat during jointing stage, watering saline-alkali water respectively in wheat planting district and to saline-alkali water 1～5cm above ground level and keep certain time, every liter of saline-alkali water comprises 26.276 grams of sodium chloride, 2.16 grams of magnesium chloride, 3.148 grams of magnesium sulfate, 1.243 grams of calcium chloride, sodium bicarbonate 0.179 gram, 0.723 gram of potassium chloride。
3. a kind of method for breeding wheat according to claim 1, it is characterized in that, improvement MS in institute step S4 includes manganese methionine 10mg/L, , zinc sulfate monohydrate 5mg/L, nucleoside hydrochlorate 17mg/L, magnesium amino acid chelate 5mg/L, Selenomethionine 5.5mg/L, phytase 0.75mg/L, medium trace element 4.5mg/L, pyridine acid chromium salt 0.065mg/L, calcium iodate 0.3mg/L, lysine 6mg/L, chelating amino acids cobalt 0.065mg/L, albumen germanium 0.75mg/L, iodine yeast 6mg/L, thiamine hydrochloride 10mg/L, pyridoxine hydrochloride 3mg/L, inositol 35mg/L, cyclohexanhexanol 27mg/L。
4. a kind of method for breeding wheat according to claim 3, it is characterized in that, 5 '-adenylic acid dipotassium that described nucleoside hydrochlorate obtains after including ribonucleic acid basic hydrolysis or sodium, 5 '-bird adenylic acid dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element includes Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2。
5. a kind of method for breeding wheat according to claim 1, it is characterised in that described Salt And Alkali Tolerance character includes any three kinds in following character:
Wheat seed: long awns, white shell and white grain；
Plant height: 65～85cm；
Fruit ear: grain number per spike 35～45, mass of 1000 kernel 45～55g, fecundity is good；Yield: 485～545kg/ mu。
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Cited By (4)
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| CN106416710A (en) * | 2016-10-09 | 2017-02-22 | 漯河市农业科学院 | Method for increasing distant hybridization setting rate of Elytrigia elongata and Triticum aestivum |
| CN107371680A (en) * | 2017-06-30 | 2017-11-24 | 南京中科水治理股份有限公司 | A kind of breeding method for strengthening the domestication of Potamogeton pectinatus L salt resistance ability |
| CN107750938A (en) * | 2017-08-29 | 2018-03-06 | 长葛市联发粮食专业合作社 | A kind of method for breeding wheat |
| CN111777462A (en) * | 2020-06-29 | 2020-10-16 | 崇左市青林金花茶开发有限公司 | Nutrient solution for increasing growth speed of golden camellia |
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| CN111777462A (en) * | 2020-06-29 | 2020-10-16 | 崇左市青林金花茶开发有限公司 | Nutrient solution for increasing growth speed of golden camellia |
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