CN103120122A - Cultivating method of high isoflavone content soybeans - Google Patents
Cultivating method of high isoflavone content soybeans Download PDFInfo
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- CN103120122A CN103120122A CN 201210517714 CN201210517714A CN103120122A CN 103120122 A CN103120122 A CN 103120122A CN 201210517714 CN201210517714 CN 201210517714 CN 201210517714 A CN201210517714 A CN 201210517714A CN 103120122 A CN103120122 A CN 103120122A
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Abstract
The invention provides a cultivating method of high isoflavone content soybeans. The cultivating method is characterized by comprising the following steps of: utilizing ripe soybean embryonic tips to obtain somatic embryos; paving the germinated embryonic tips of soybean seeds in a callus induction culture medium to form calluses through dark culture; paving the obtained calluses into a somatic embryo induction culture medium to induce the generation of somatic embryos; and hybridizing after extracting the high-quality somatic embryos and cultivating the variety with the high isoflavone content. According to the high isoflavone content soybeans cultivated by the method, the content of the isoflavone is improved to eight over one thousand and is improved by 13.3 times when being compared with the content of previous products.
Description
Technical field
The present invention relates to the agricultural industry technical field, specifically relate to the cultivating method of a kind of homoisoflavone soybean.
Background technology
Soybean originates from China, and the blood relationship of external the cultivated soybean is nearly all from China, and China has 90% of world's Wild Soybean Resources, utilizes dissimilar soybean resource to carry out the innovation research of soybean product processing significant.In promoting soybean varieties and landrace at present, high protein, high oil, the resource of homoisoflavone and special trait is very abundant, exploitation adapts to different crowd, and the different products that need can be selected according to the characteristics of resource itself, and need not to add in addition other compositions, make the traditional food back to nature under modern crafts.Isoflavones is a kind of in flavonoids, mainly is present in leguminous plant, and isoflavones is a class secondary metabolite that forms in soybeans they grow.Because being extracts from plant, with oestrogenic hormone, analog structure is arranged, therefore isoflavones claims again phytoestrogen, can make up the defective of women's female sex hormone hyposecretion after 30 years old, improve moisture of skin and elasticity situation, alleviate climacteric metancholia and improve osteoporosis, making the women reproduce the youth glamour.The estrogen action of isoflavones has influence on hormone secretion, metabolic biology is active, protein is synthetic, growth factor activity, is natural cancer chemopreventive agent.The soybean varieties of the high isoflavone-containing of this research and utilization is produced soybean product, agricultural production and product processing are combined, realized that the sterling kind is produced and also given new function for original traditional processed soybean food, satisfy the hope that the people yearn for healthy and health food, also improved scientific and technological content and the market competitiveness of product.The appearance of homoisoflavone soybean product is exactly that she can also have health care for nutrition and the idea of feeding oneself with changing people's food, prevention and treat the function of disease.The research will have higher social effect and actual application value.Along with improving constantly of people's lives, also just more and more higher to the requirement of food nutrition and self health care, the exploitation by functional product can play to the development of bean product industry very large facilitation, gives the input of bean product industry a kind of new principle.This traditional food of bean product can be risen to a new height.Simultaneously market prospects are also unusual Worth Expectings.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the low defective of isoflavone content in prior art, and the cultivating method of a kind of homoisoflavone soybean is provided, and the method can improve the content of isoflavone from soybean greatly, makes the isoflavones extraction effect remarkable.
The cultivating method of homoisoflavone soybean, it is characterized in that, utilize ripe soybean embryo point to obtain somatic embryo, embryo point by the soya seeds that will sprout is tiled in that in calli induction media, dark the cultivation generates callus, and the callus that obtains is tiled in the generation of inductor blast in the somatic embryo inducement medium, hybridize after extracting the high-quality somatic embryo, cultivate the higher kind of isoflavone content.
Beneficial effect, by the homoisoflavone soybean that this method is cultivated, its isoflavone content rises to 8/1000ths, compares original product content and promotes 13.3 times.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with concrete example, further set forth the present invention.
Isoflavones is the natural active matter relevant with soybean resistance and nodule formation, has anti-cancer, the effect of antibiotic, anti-oxidant, anti-hemolysis and female sex hormone.Isoflavones mainly is present in the plumular axis of soya seeds, and its content is generally 5-6 times of cotyledon, and kind difference very big (more than 10 times).
In traditional soybean product, isoflavone content is 0.6/1000th, and by the homoisoflavone soybean that this method is cultivated, its isoflavone content rises to 8/1000ths, compares original product content and promotes 13.3 times.
Cultural method is: utilize ripe soybean embryo point to obtain somatic embryo, embryo point by the soya seeds that will sprout is tiled in that in calli induction media, dark the cultivation generates callus, and the callus that obtains is tiled in the generation of inductor blast in the somatic embryo inducement medium.Hybridize after extracting the high-quality somatic embryo, cultivate the higher kind of isoflavone content.
Described calli induction media is used for evoked callus and forms, and prevents brown stain.take MS medium mother liquor as the basis, sequentially add macroelement 20 * mother liquor 100mL in clean aluminum pot, trace element 100 * mother liquor 20mL, molysite 100 * mother liquor 20mL, vitamin 100 * mother liquor 20mL, inositol 200 * mother liquor 10mL, glycine 200 * mother liquor 10mL, after preparation obtains the MS medium like this, the BA8mL that adds again 0.5mgL-1, 0.5mgL-1 NAA8mL. then add the approximately distilled water of 2/3-3/4 of actual preparation culture volume, after adding 40g sucrose, stirring is dissolved it, adjust the pH value with the HCl of the NaOH of 0.5molL-1 and 0.5molL-1 and add 14g agar to 5.8-6.0., aluminum pot is placed on electric furnace, agitating heating is dissolved agar fully, then be settled to final volume 2L with distilled water, continue after heating a few minutes make it to mix, namely get calli induction media, be sub-packed in the triangular flask that holds callus inducing medium.
The technical staff of the industry should understand; the present invention is not subjected to the restriction of above-mentioned example; that describes in above-mentioned example and specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (1)
1. the cultivating method of homoisoflavone soybean, it is characterized in that, utilize ripe soybean embryo point to obtain somatic embryo, embryo point by the soya seeds that will sprout is tiled in that in calli induction media, dark the cultivation generates callus, and the callus that obtains is tiled in the generation of inductor blast in the somatic embryo inducement medium, hybridize after extracting the high-quality somatic embryo, cultivate the higher kind of isoflavone content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210517714 CN103120122A (en) | 2012-12-05 | 2012-12-05 | Cultivating method of high isoflavone content soybeans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210517714 CN103120122A (en) | 2012-12-05 | 2012-12-05 | Cultivating method of high isoflavone content soybeans |
Publications (1)
| Publication Number | Publication Date |
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| CN103120122A true CN103120122A (en) | 2013-05-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201210517714 Pending CN103120122A (en) | 2012-12-05 | 2012-12-05 | Cultivating method of high isoflavone content soybeans |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106174043A (en) * | 2016-06-29 | 2016-12-07 | 南京农业大学 | A kind of big tall scrawny person is enriched with the technology of isoflavone |
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2012
- 2012-12-05 CN CN 201210517714 patent/CN103120122A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106174043A (en) * | 2016-06-29 | 2016-12-07 | 南京农业大学 | A kind of big tall scrawny person is enriched with the technology of isoflavone |
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Application publication date: 20130529 |