CN104396729B - A kind of homoisoflavone soybean breeding method - Google Patents
A kind of homoisoflavone soybean breeding method Download PDFInfo
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- CN104396729B CN104396729B CN201410667433.2A CN201410667433A CN104396729B CN 104396729 B CN104396729 B CN 104396729B CN 201410667433 A CN201410667433 A CN 201410667433A CN 104396729 B CN104396729 B CN 104396729B
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Abstract
The invention discloses and belong to a kind of homoisoflavone soybean breeding method that breeding technical field can be false proof.Hybridize for coffee-like homoisoflavone soybean parents with the homoisoflavone soybean parents that Newborn Leaves is bright-coloured yellow flag with soybean kernel, F1 generation selfing, F2 is in colony, the seedling selecting seedling Newborn Leaves to be bright-coloured yellow, by Plant colonization, individual plant harvest-seed extraction is pressed time ripe, seed seed coat of selecting and remain isozygotys for coffee-like individual plant, F3 is in strain, leave bright-coloured yellow flag and seed seed coat is coffee-like homozygous lines, analyze detection seed isoflavone content, retain the content strain more than 5 ‰, at F4 in strain, carry out protein and fat content analysis, the strain of high-quality of selecting and remain, selfing repeated for 2～3 generations, carry out systematic breeding, in acquisition, high yield and high quality can false proof homoisoflavone soybean.The homoisoflavone specialized soybean that the present invention is bred as, has the special marking that can intuitively identify that Newborn Leaves is yellow, it is simple to authenticity of products is screened.
Description
Technical field
The invention belongs to crossbreeding technology field, be specifically related to a kind of homoisoflavone soybean breeding method that can be false proof.
Background technology
Along with improving constantly of living standards of the people, people are the most clear to healthy understanding.It is equally known that with the progressively growth of female age, within 30 years old, later women has stepped into the stage of aging the most at leisure.Experts point out that the basic reason causing female aging, it is simply that because the deficiency of estrogen, secretion reduce or imbalance in women body.
Isoflavones is also known as phytoestrogen.Rich in natural homoisoflavone element, it is possible to make up the defect of women female hormone hyposecretion, improve moisture of skin and elastic situation, alleviate climacteric metancholia and improve osteoporosis, making women reproduce youth glamour！Estrogen action has influence on hormone secretion, metabolic biology activity, protein synthesis, growth factor activity, is natural cancer chemopreventive agent.Substantial amounts of research shows: isoflavones has following major physiological effect: balance the estrogen level in women body；Anti-aging, beautifying face and moistering lotion；Prevention breast cancer；Prevent and improve female dimacteric syndrome；Improve menstrual period uncomfortable；Pre-anti-cancer, antitumor etc..
At present, the research and development of isoflavones are extracted as main line with processing, and product mostly typically is pharmaceutical intermediate and eats in special population as health product, little from the angle research of homoisoflavone breed breeding.Either local Soybean Germplasm detection is shown by traditional soybean product country of consumption (China, Japan, Korea S) or main Soybean production state (U.S., Canada, Brazil, India), the isoflavone content of soybean culture kind is on the low side, and what isoflavone content was the highest mostly also is wild soybean.The most within this context, carry out the seed selection of homoisoflavone content functional soy new varieties, it is intended to take the strategy of diet, supplement isoflavones from food source and prevent the generation of relevant disease.
It should be noted that the soybean that existing market circulates, seed coat color is common yellow, the leaf color of field seedling and plant is also green, it is unfavorable for the truth identification of the functional soys such as homoisoflavone, the most adulterated when commercially producing sale, cause the confusion in market.The most within this context, the present invention proposes that a kind of production can monitor, product can recognize that the selection of homoisoflavone soybean, is conducive to planning in an orderly manner market, forms property right protection mechanism.
Summary of the invention
It is an object of the invention to provide a kind of homoisoflavone soybean breeding method that can be false proof.
A kind of can be false proof homoisoflavone soybean breeding method, carry out in accordance with the following steps:
1) with the homoisoflavone soybean parents that newborn blade is bright-coloured yellow flag, being coffee-like homoisoflavone soybean parents hybridization with soybean kernel, hybridization obtains F1 generation seed；
2) F1 generation selfing, it is thus achieved that F2 is for seed；
3) at F2 in colony, seedbed seedling is carried out, in the seedling 3-4 leaf phase, the seedling first selecting seedling Newborn Leaves to be bright-coloured yellow, carry out field planting by every cave 1 strain and move into land for growing field crops, time ripe, press individual plant harvest-seed extraction, seed seed coat of selecting and remain isozygotys for coffee-like individual plant；
4) at F3 in strain, by the soybean kernel sowing of 1, the every cave of strain.Seedling Stage, first staying the seedling selecting whole strain each seedling Newborn Leaves interior to be bright-coloured yellow, press individual plant harvest-seed extraction time ripe, in strain of selecting and remain, each individual plant seed seed coat is coffee-like strain, analyze detection seed isoflavone content, retain the isoflavone content strain more than 5 ‰；
5) at F4 in strain, by the seed sowing of 1, the every cave of strain, results threshing, selecting Newborn Leaves is bright-coloured yellow flag, seed coffee color and the isoflavone content strain more than 5 ‰, measures protein and fat content, the strain of high-quality of selecting and remain；
6) F5～F7 self progeny, repeat 5) step, carry out systematic breeding, it is thus achieved that middle high yield and high quality can the consistent soybean of false proof homoisoflavone stable content.
Described newborn blade has the homoisoflavone soybean parents of bright-coloured yellow flag for growing body source 1, soybean kernel be coffee-like homoisoflavone soybean parents for growing body source 2, all applied for New species right in plant protection.
Described homoisoflavone stable content is consistent, refers to 2 years isoflavone contents of more than 2 stable more than 5 ‰.
The determination method of isoflavone content, participation national standard: the assay method-high performance liquid chromatography of isoflavones in GB/T23788-2009 health food.
F5～F7 self progeny, select individual plant to carry out the systematic breeding of target isoflavone content and economical character, excellent in select excellent, general seed selection 2～3 generation.
Beneficial effects of the present invention: the homoisoflavone specialized soybean that the present invention is bred as, in process of production, has the special marking that can intuitively identify that Newborn Leaves is yellow, and convenient production purifies discriminating, and seed is coffee-like, it is simple to authenticity of products is screened.
Accompanying drawing explanation
Fig. 1 be the newborn blade of embodiment 1 seed selection be bright-coloured yellow flag and common green soybean form.
Fig. 2 be the soybean kernel seed coat of embodiment 1 seed selection be the soybean form of coffee-like and common yellow.
Detailed description of the invention
The present invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
Embodiment 1
Breeding material:
Newborn blade has the homoisoflavone soybean parents of bright-coloured yellow flag for growing body source 1, and soybean kernel is that coffee-like homoisoflavone soybean parents is for growing body source 2.Growing body source 1 and grow the homoisoflavone soybean that body source 2 is the autonomous seed selection of company, content the most stably more than 5 ‰, has all been applied for New species right in plant protection, can be provided by company oneself.
A kind of can be false proof homoisoflavone soybean breeding method, including:
Summer in 2009, Zhejiang Hangzhou, with the homoisoflavone soybean parents that newborn blade is bright-coloured yellow flag, was coffee-like homoisoflavone soybean parents hybridization with soybean kernel, and hybridization obtains 50, F1 generation seed；
Lingshui, Hainan in spring in 2010, F1 generation selfing, it is thus achieved that F2 is for seed strain more than 4500；
Summer in 2010 Zhejiang Hangzhou, at F2 in colony, carry out seedbed seedling, in the seedling 3-4 leaf phase, seedling 1081 strain first selecting seedling Newborn Leaves to be bright-coloured yellow, carries out field planting by every cave 1 strain and moves into land for growing field crops, survive 1062 strains, pressing individual plant harvest-seed extraction time ripe, seed seed coat of selecting and remain isozygotys for coffee-like individual plant 253 strain；
Lingshui, Hainan in spring in 2011, at F3 in strain, by the soybean kernel sowing of 1, the every cave of strain.Seedling Stage, first staying the seedling selecting whole strain each seedling Newborn Leaves interior to be bright-coloured yellow, press individual plant harvest-seed extraction time ripe, in strain of selecting and remain, each individual plant seed seed coat is coffee-like strain, analyze detection seed isoflavone content, retain the isoflavone content strain more than 5 ‰ 49；
Summer in 2011 Zhejiang Hangzhou, at F4 in strain, by the seed sowing of 1, the every cave of strain, results threshing, in strain of selecting and remain, Newborn Leaves is bright-coloured yellow flag, seed coffee color and the isoflavone content strain more than 5 ‰, measure protein and fat content, the strain of high-quality of selecting and remain 26；
2012-2013 Zhejiang and Hainan, F5～F7 self progeny, repeat the step of (5), carry out systematic breeding, it is thus achieved that middle high yield and high quality can 1-YCHISA01 of false proof homoisoflavone soybean.
The feature main for YCHISA01 of above-mentioned steps seed selection shows themselves in that new life blade in the time of infertility is respectively provided with bright-coloured yellow flag (as shown in Figure 1), and seed seed coat is distinguished coffee color (as shown in Figure 2), and isoflavone content is 5.6 ‰.
Claims (2)
1. a homoisoflavone soybean breeding method, it is characterised in that carry out in accordance with the following steps:
1) being bright-coloured yellow using newborn blade grows body source 1 as the homoisoflavone soybean parents of mark, and greatly
Beans seed is that coffee-like homoisoflavone soybean parents grows body source 2 hybridization, and hybridization obtains F1 generation seed；
2) F1 generation selfing, it is thus achieved that F2 is for seed；
3) at F2 in colony, carry out seedbed seedling, in the seedling 3-4 leaf phase, first select seedling Newborn Leaves
For the seedling of bright-coloured yellow, carry out field planting by every cave 1 strain and move into land for growing field crops, time ripe, press individual plant harvest-seed extraction,
Seed seed coat of selecting and remain isozygotys for coffee-like individual plant；
4) at F3 in strain, by the soybean kernel sowing of 1, the every cave of strain；Seedling Stage, first stay select whole
In strain, each seedling Newborn Leaves is the seedling of bright-coloured yellow, presses individual plant harvest-seed extraction, strain of selecting and remain time ripe
Interior each individual plant seed seed coat is coffee-like strain, analyzes detection seed isoflavone content, retains isoflavones and contains
The amount strain more than 5 ‰；
5) at F4 in strain, by the seed sowing of 1, the every cave of strain, results threshing, Newborn Leaves is selected
For bright-coloured yellow, seed coffee color and the isoflavone content strain more than 5 ‰, measure protein and fat contains
Amount, the strain of high-quality of selecting and remain；
6) F5～F7 self progeny, repeat 5) step, carry out systematic breeding, it is thus achieved that middle high yield and high quality
The consistent soybean of homoisoflavone stable content.
A kind of homoisoflavone soybean breeding method, it is characterised in that described
Homoisoflavone stable content is consistent, refers to 2 years isoflavone contents of more than 2 stable more than 5 ‰.
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