CN104322367A - Method for breeding soybeans containing high isoflavone content - Google Patents
Method for breeding soybeans containing high isoflavone content Download PDFInfo
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- CN104322367A CN104322367A CN201410667435.1A CN201410667435A CN104322367A CN 104322367 A CN104322367 A CN 104322367A CN 201410667435 A CN201410667435 A CN 201410667435A CN 104322367 A CN104322367 A CN 104322367A
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Abstract
The invention discloses a method for breeding soybeans with high isoflavone content, which belongs to the technical field of cross breeding. The method adopts the ways of mutagenesis treatment and continuous selfing to breed soybeans containing high isoflavone content. By using grain color related to the isoflavone, the process of pre-screening is carried out, so that the method is simple, economical and effective and suitable for large-scale breeding; the bred special soybeans containing high isoflavone content has the advantages of intuitively identifying grain coat marks, facilitating special identification and also facilitating the retaining and screening; the bred special soybeans containing high isoflavone content are high in isoflavone content and meanwhile have the advantages of high yield, high quality, high disease-resistance and adaptability, so that the increasing of production and income of the new functional breed can be well reflected.
Description
Technical field
The invention belongs to crossbreeding technology field, be specifically related to the selection of a kind of homoisoflavone content soybean.
Background technology
Along with improving constantly of living standards of the people, people are also day by day clear to the understanding of health.As you know, with the progressively growth of female age, within 30 years old, later women has just stepped into the old and feeble stage at leisure.Expert points out: the basic reason causing female aging, is exactly because the deficiency of women's body inner estrogen, secretion minimizing or imbalance.
Isoflavones is also known as phytoestrogen.Be rich in natural homoisoflavone element, the defect of women's female sex hormone hyposecretion can be made up, improve moisture of skin and elasticity situation, alleviate climacteric metancholia and improve osteoporosis, making women reproduce youth glamour! Estrogen action has influence on hormone secretion, metabolic biology is active, protein synthesizes, growth factor activity, is natural cancer chemopreventive agent.Large quantifier elimination shows: isoflavones has following major physiological effect: the estrogen level in balance women body; Anti-ageing, beautifying face and moistering lotion; Prevention breast cancer; Prevent and improve female dimacteric syndrome; Improve discomfort in menstrual period; Pre-anti-cancer, antitumor etc.
At present, the research and development of isoflavones are extracted as main line with processing, and it is edible in special population as health product that product mostly generally is pharmaceutical intermediate, little from the angle research of homoisoflavone breed breeding.No matter be that traditional soybean product country of consumption (China, Japan, Korea S) or main Soybean production state (U.S., Canada, Brazil, India) show local Soybean Germplasm detection, the isoflavone content of soybean culture kind is on the low side, and isoflavone content is high extremely individually mostly also is wild soybean.Just within this context, carry out the seed selection of homoisoflavone content functional soy new varieties, be intended to the strategy taking diet, supplement isoflavones from food source and prevent the generation of relevant disease.
Summary of the invention
The object of the invention is to the selection of openly a kind of homoisoflavone content soybean.
A selection for homoisoflavone content soybean, carries out in accordance with the following steps:
3) material based on the higher soybean of isoflavone content, takes mutagenic treatment, obtains M1 for seed;
4) M1 is for the plantation of every cave 2-3 grain, and selfing obtains M 2 generation;
3) at M2 in colony, by 1, every cave seed single-strain planting, screening seed is coffee-like sudden change individual plant, measures isoflavone content, the individual plant of selected homoisoflavone content;
4) at M3 in strain, by strain 1, every cave seed single-strain planting, the mutantion line of selected isoflavones sudden change stably express, measures to stay and selects the mutantion line of content more than 5 ‰;
5) at M4 in strain, by the plantation of 2 ~ 3 seeds in the every cave of strain, evaluate yield and quality, selected coffee-like and isoflavone content is more than 5 ‰ and the mutantion line taken into account of quality and yield;
6) at M5 ~ M7 in strain, different location repeats 5) step, carry out systematic breeding, obtain the high yield and high quality specialized soybean that homoisoflavone stable content is consistent.
Described basic material is Southern soybean regional strain ZN03.
Described mutagenic treatment is physical mutagen cobalt-60 gamma rays, treatment dosage 120Gy.About utilizing the variation of mutagenic obtained seed color of the leather, as long as appropriate dosage and the mutagenesis screening colony of selection are enough large, had in the past and successfully studied report, in detail see Chen Xuezhen, Xie Hao, Luan Tao, wait the genetic variation of soybean M2 economical character after the process of .60Co-gamma-ray irradiation. Beijing Agricultural College's journal, 2002,17 (3): 1-5.
Described homoisoflavone stable content is consistent, refers to 2 years isoflavone contents of more than 2 and is stabilized in more than 5 ‰.
The determination method of isoflavone content, participates in national standard: assay method one high performance liquid chromatography of isoflavones in GB/T23788-2009 health food.
M5 ~ M7 self progeny, select individual plant to carry out the systematic breeding of target isoflavone content and economical character, select excellent in excellent, general seed selection 2 ~ 3 generation.
Above-described homoisoflavone stable content is consistent, refers to 2 years isoflavone contents of more than 2 and is stabilized in more than 5 ‰.
Beneficial effect of the present invention: due to isoflavone content determination method, process very complicated, cost is high, be difficult to the rapid analysis carrying out large sample, the seed look that employing of the present invention is relevant to isoflavones carries out prescreen, and method is simple, economical and effective, is suitable for large Breeding Scale; The homoisoflavone specialized soybean be bred as, has and intuitively can identify that seed seed coat marks, and facilitates specific identification and be convenient to produce fidelity to screen; The homoisoflavone specialized soybean be bred as, while maintenance homoisoflavone content, has effectively taken into account high yield, high-quality, disease resistance and adaptability, better can embody functional new varieties increasing both production and income.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
With southern autumn soybean regional strain ZN03 for starting material, seed is yellow, isoflavone content 2.6 ‰, adopts cobalt-60 gamma rays irradiation of 120Gy, treatment dosage, dose rate 20Gy/min; Autumn in summer in 2009 Zhejiang Hangzhou, every cave 2-3 grain plantation M
1, all seeds of mixed receipts; Lingshui, Hainan in spring in 2010, by 1, every cave seed single-strain planting M
2, screening seed is coffee-like sudden change individual plant 35, measures isoflavone content, the individual plant that selected isoflavone content significantly improves 7; Autumn in summer in 2010 Zhejiang Hangzhou, by 1, the every cave of strain seed single-strain planting M
3, the mutantion line 6 of selected isoflavones sudden change stably express, wherein content is more than 3 of 5 ‰ mutantion lines; Autumn in summer in 2011 Zhejiang Hangzhou, by 2 ~ 3, the every cave of strain seed plantation M
4, evaluate yield and quality, selected coffee-like and isoflavone content is about 5.5 ‰ and quality and yield 1 of mutantion line of taking into account, numbering CHIS-01.Xia Qiu Zhejiang in 2012, at Zhejiang Hangzhou, Linan and ground, Fuyang three plantation M
6cHIS01, evaluate specificity, stability and uniformity; Xia Qiu Zhejiang in 2013, at Zhejiang Hangzhou, Linan and ground, Fuyang three plantation M
7cHIS01, evaluate specificity, stability and uniformity; Autumn and winter in 2013 Zhejiang Hangzhou, CHIS01 name grow No. 2, body source, carry out the edible test of special population; Within 2014, submit Plant new variety protection application-No. 2, taste body source to.
Claims (4)
1. a selection for homoisoflavone content soybean, is characterized in that, carries out in accordance with the following steps:
1) material based on the higher soybean of isoflavone content, takes mutagenic treatment, obtains M1 for seed;
2) M1 is for the plantation of every cave 2-3 grain, and selfing obtains M2 generation;
3) at M2 in colony, by 1, every cave seed single-strain planting, screening seed is coffee-like sudden change individual plant, measures isoflavone content, the individual plant of selected homoisoflavone content;
4) at M3 in strain, by strain 1, every cave seed single-strain planting, the mutantion line of selected isoflavones sudden change stably express, measures to stay and selects the mutantion line of content more than 5 ‰;
5) at M4 in strain, by the plantation of 2 ~ 3 seeds in the every cave of strain, evaluate yield and quality, selected coffee-like and isoflavone content is more than 5 ‰ and the mutantion line taken into account of quality and yield;
6) at M5 ~ M7 in strain, different location repeats 5) step, carry out systematic breeding, obtain the high yield and high quality specialized soybean that homoisoflavone stable content is consistent.
2. the selection of a kind of homoisoflavone content soybean according to claim 1, it is characterized in that, described basic material is Southern soybean regional strain ZN03.
3. the selection of a kind of homoisoflavone content soybean according to claim 1, it is characterized in that, described mutagenic treatment, is physical mutagen cobalt-60 gamma rays, treatment dosage 120Gy.
4. the selection of a kind of homoisoflavone content soybean according to claim 1, is characterized in that, described homoisoflavone stable content is consistent, refers to 2 years isoflavone contents of more than 2 and is stabilized in more than 5 ‰.
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| CN201410667435.1A CN104322367A (en) | 2014-11-21 | 2014-11-21 | Method for breeding soybeans containing high isoflavone content |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006459A (en) * | 2018-07-24 | 2018-12-18 | 重庆市农业科学院 | A kind of soybean breeder method of protein content stable type |
| CN111742841A (en) * | 2020-08-04 | 2020-10-09 | 中国科学院东北地理与农业生态研究所 | Method for mutagenizing soybeans by heavy ion beam C irradiation |
-
2014
- 2014-11-21 CN CN201410667435.1A patent/CN104322367A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006459A (en) * | 2018-07-24 | 2018-12-18 | 重庆市农业科学院 | A kind of soybean breeder method of protein content stable type |
| CN111742841A (en) * | 2020-08-04 | 2020-10-09 | 中国科学院东北地理与农业生态研究所 | Method for mutagenizing soybeans by heavy ion beam C irradiation |
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Application publication date: 20150204 |