CN102498847B - Screening method of advantage-based stock seed of isatis root - Google Patents
Screening method of advantage-based stock seed of isatis root Download PDFInfo
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- CN102498847B CN102498847B CN 201110317155 CN201110317155A CN102498847B CN 102498847 B CN102498847 B CN 102498847B CN 201110317155 CN201110317155 CN 201110317155 CN 201110317155 A CN201110317155 A CN 201110317155A CN 102498847 B CN102498847 B CN 102498847B
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Abstract
The invention relates to a screening method of advantage-based stock seed of isatis root, and is applicable to improved variety breeding of isatis root. The screening method comprises the steps of: collecting isatis root germ plasma resources and building a resource garden; evaluating individuals and small groups of different germ plasma species according to the individual yield, inherent quality, commercial product characters and reproductive biological characteristics, carrying out germ plasma breeding and screening for continuous two years to obtain advantage-based stock seed of isatis root. The obtained advantage-based stock seed has the advantages that the principal root is long and thick, the leaves are long and narrow, the leaves are dark green, the individual has multiple leaves, and the dry weights of the leaves and root of the individual are high; the seeds are long and wide, full in grains and high in weight of thousand grains; after being conventionally sundried, the isatis root has adenosine content of 6.0-7.5% and alcohol extract content of 25-33%; and isatis leaf has indirubin content of 0.05-0.06% and alcohol extract content of 28-30%.
Description
Technical field
The present invention relates to a kind of screening technique of advantage-based stock seed of isatis root, be exclusively used in stable, high, the quality better of yielding ability of screening proterties, in order to the advantage-based stock seed of isatis root of fine-variety breeding.
Background technology
Radix Isatidis Radix Isatidis derives from the dry root of cruciferae isatis Isatis indigotica Fort. for being China's parts of generic medicinal plants.Radix Isatidis bitter, cold in nature, the thoughts of returning home, stomach warp, have clearing heat and detoxicating, the effect of cool blood relieve sore throat.Modern chemistry and pharmacological research confirm, contain alkaloids, organic acid, amino acids, sterols, adenosine and polysaccharose composition in the Radix Isatidis; Have antibiotic, antiviral activity, can strengthen immunity of organisms, and can prevent that SARS is popular.Multiplex in diseases such as influenza, mumps, chronic pharyngitis, anemopyretic colds clinically.
Radix Isatidis is as one of China's bulk medicinal materials, and social required quantity is larger, and annual domestic and international market amount is 3000～4000 tons, and wherein about 25% for export.Woaded blue belongs to dispersed species, at China's most areas artificial cultivation is arranged all, but even to this day, cultivated form is very chaotic between each main producing region, cause Radix Isatidis that produce various places uneven on commodity property and interior quality, be difficult to guarantee that this root yield is stable, quality controllable; At present, most researchs all concentrate on the aspects such as field standardized planting technology (Chen Yuhang etc., planting density on the woaded blue growth dynamically and the impact of output, CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2008,33 (22): 2599-2602; Chen Yuhang etc., sowing time is on the impact of Radix Isatidis morphological feature, output and quality. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2009,34 (21): 2709-2712; Yan Xiangwei etc. planting density is on the impact of indigo red Dynamic Accumulation in Radix Isatidis alcohol extract, adenosine and the folium isatidis. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2011,36 (8): 977-979), but about the research report of germplasm comparation and assessment and breed breeding aspect less (Guo Qiaosheng etc. the different germplasm growth characteristics of woaded blue and and the correlation analysis of single plant yield thereof. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2009,34 (16): 2034-2038.); Therefore, be badly in need of carrying out advantage-based stock seed of isatis root evaluation and screening operation, provide high-quality to breed material in the hope of the seed selection for Radix Isatidis good quality and high output kind.
Summary of the invention
1, technical problem
It is chaotic to the present invention be directed to Radix Isatidis cultivated form, and root yield and its product amount are difficult to be guaranteed, and a kind of method of screening the former germplasm of Radix Isatidis advantage base is provided, and launches extensively to collect, set up Germplasm Resources for the existing germ plasm resource of Radix Isatidis; Existing germ plasm resource is carried out output, commodity property, reproductive biology and interior quality compare, invent and a kind ofly breed the method for material for the Radix Isatidis breed breeding provides high-quality.
2, technical scheme
The present invention is by the following technical solutions: a kind of screening technique of advantage-based stock seed of isatis root, and it may further comprise the steps:
Step 1, Radix Isatidis germ plasm resource is collected and is set up from Germplasm Resources: at first collect different germplasm Radix Isatidis, select full, of the same size silicle (practise in the production and claim seed), a minute residential quarter is seeded in the Germplasm Resources, inserts card mark and carries out conventional field management;
Step 2, Radix Isatidis germplasm morphological character comparation and assessment: in the Radix Isatidis Germplasm Resources, compare by main root length, root diameter, the proterties such as leaf is long, leaf is wide, single-strain blade number, individual plant leaf dry weight, individual plant root dry weight, complete stool dry weight for different germplasms individualities and microcommunity: select the long 29～32cm of main root, root diameter 0.9～1.1cm, the long 23～26cm of leaf, the wide 4～5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, complete stool dry weight 17～20g;
Step 3, Radix Isatidis germplasm interior quality comparation and assessment: mid-November then, in each residential quarter, take the Radix Isatidis plant of some, root division and leaf two parts, after routine is dried, Radix Isatidis adenosine content 6.0%～7.5%, alcohol extract content 25～33%, folium isatidis Content Determination of Indirubin 0.05%～0.06%, folium isatidis alcohol extract 28%～31%;
Step 4, Radix Isatidis germplasm reproductive characteristic comparation and assessment: will remain basic former germplasm original place and keep, and survive the winter, and before in mid-March, the 2nd, each germplasm minute residential quarter was transplanted, seeding row spacing 30 * 50cm is pressed in field planting, green separation net is set simultaneously, prevents mutually pollination between each germplasm; Observe their the Characters and stability separately, selecting to have plant height is 105～110cm, branch amount 6～12, the long 1.5～1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, thousand grain weigth 10～12g;
Step 5, breed and screen through continuous 2 years Radix Isatidis germplasm, final definite former germplasm morphological characters of advantage base: the long 29～32cm of main root, root diameter 0.9～1.1cm, the long 23～26cm of leaf, the wide 4～5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, complete stool dry weight 17～20g; The biology of reproduction proterties: plant height is 105～110cm, branch amount 6～12, the long 1.5～1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, thousand grain weigth 10～12g; Interior quality: Radix Isatidis adenosine content 6.0%～7.5%, alcohol extract content 25%～33%, folium isatidis Content Determination of Indirubin 0.05%～0.06%, folium isatidis alcohol extract 28%～31%.
Beneficial effect
The screening technique of advantage-based stock seed of isatis root provided by the invention compared with prior art has following advantage and good effect:
For guaranteeing the Radix Isatidis yield and quality, the present invention is on Research foundation for many years, comprehensive output, interior quality (adenosine, alcohol extract, indigo red), commodity property (main root is long, main root is wide, leaf is long, leaf wide), (fruit is long for reproductive biology, fruit is wide, seed is long, seed is wide, thousand grain weigth) etc. index is carried out evaluation study to the former germplasm of Radix Isatidis base, filter out therefrom that proterties is stable, quality better, the former germplasm of advantage base that output is high, in order to breed material for the breed breeding of Radix Isatidis provides.
The former germplasm of Radix Isatidis base by institute of the present invention seed selection has following stable character: the long 29～32cm of main root, root diameter 0.9～1.1cm, long 23～the 26cm of leaf, the wide 4～5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, complete stool dry weight 17～20g, plant height are 105～110cm, branch amount 6～12, long 1.5～the 1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, thousand grain weigth 10～12g;
Active component adenosine, alcohol extract, Content Determination of Indirubin measurement result: the Radix Isatidis adenosine content is 6.0%～7.5%, alcohol extract content is 25%～33%, the folium isatidis Content Determination of Indirubin is 0.05%～0.06%, and folium isatidis alcohol extract content is 28%～31%.
Description of drawings
Fig. 1 is advantage-based stock seed of isatis root screening process figure
Embodiment
Anhui Province's Fuyang City is one of China Radix Isatidis major production areas, and our unit carries out advantage-based stock seed of isatis root screening and fine-variety breeding research in beginning in 2006 in this area.
At first collect different germplasm Radix Isatidis, select full, of the same size silicle (practise in the production and claim seed), a minute residential quarter is seeded in the Germplasm Resources, inserts card mark and carries out conventional field management;
In the Radix Isatidis Germplasm Resources, individuality and microcommunity for different germplasms are appraised through comparison by main root length, root diameter, the proterties such as leaf is long, leaf is wide, single-strain blade number, individual plant leaf dry weight, individual plant root dry weight, complete stool dry weight: select the long 29～32cm of main root, root diameter 0.9～1.1cm, long 23～the 26cm of leaf, wide 4～the 5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, the germplasm of complete stool dry weight 17～20g;
Mid-November then, take the Radix Isatidis germplasm plant of some in each residential quarter, root division and leaf two parts, after drying, routine measures, selecting the Radix Isatidis adenosine content is 6.0%～7.5%, alcohol extract content is 25%～33%, and the folium isatidis Content Determination of Indirubin is 0.05%～0.06%, and folium isatidis alcohol extract content is 28%～31% germplasm;
To remain basic former germplasm original place and keep, and survive the winter, and before in mid-March, the 2nd, the former germplasm of a base minute residential quarter was transplanted, field planting, seeding row spacing is 30 * 50cm, and green separation net is set simultaneously, prevents mutually pollination between each germplasm; Observe their the Characters and stability separately, the selection plant height is 105～110cm, branch amount 6～12, the long 1.5～1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, the germplasm of thousand grain weigth 10～12g; Breed and screen through continuous 2 years Radix Isatidis germplasms, the former germplasm proterties of advantage base is stable to be occurred, and reaches 90% with above-mentioned proterties uniformity;
Compare (table 1, table 2, table 3) with existing conventional cultivation type, the former germplasm main root of advantage base is long, root is thick, blade is long and narrow, dark green leaf color, single-strain blade are many, individual plant leaf dry weight and root dry weight are large; Seed is long and wide, full grains, thousand kernel weight are large; The former germplasm output of advantage base is higher by 23% than conventional cultivation type; Reach 7.5% through the conventional Radix Isatidis adenosine content that dries, alcohol extract content is 32.11%; The folium isatidis Content Determination of Indirubin reaches 0.06%, and alcohol extract content is 30.19%; Exceed 27%, 13%, 20%, 14% than existing cultivated form respectively; In Radix Isatidis standardized planting base, Fuyang, breed and appraise through comparison by continuous 2 years germplasms, it is stable to filter out the former proterties of Radix Isatidis germplasm base, than the conventional cultivation type, its output, commodity property and interior quality all have clear superiority, can be fine-variety breeding provides preciousness to breed material, to finish the situation of current Radix Isatidis production base cultivated form confusion, realizes producing the optimization of cultivar.
The different germplasm type of table 1 Radix Isatidis main plant proterties is (mean value) relatively
The different germplasm type of table 2 Radix Isatidis biology of reproduction proterties is (mean value) relatively
The different germplasm type output of table 3 Radix Isatidis and interior quality be (%DW, n=3) relatively
Claims (1)
1. the screening technique of an advantage-based stock seed of isatis root, it may further comprise the steps:
Step 1, Radix Isatidis germ plasm resource is collected and set up from Germplasm Resources: at first collect different germplasm Radix Isatidis, select full, of the same size silicle, a minute residential quarter is seeded in the Germplasm Resources, inserts card mark and carries out conventional field management;
Step 2, Radix Isatidis germplasm morphological character comparation and assessment: in the Radix Isatidis Germplasm Resources, for different germplasms individual and microcommunity by main root length, root diameter, leaf is long, leaf is wide, single-strain blade number, individual plant leaf dry weight, individual plant root dry weight, complete stool dry weight proterties compare: the selection main root is grown 29～32cm, root diameter 0.9～1.1cm, long 23～the 26cm of leaf, wide 4～the 5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, the germplasm of complete stool dry weight 17～20g;
Step 3, Radix Isatidis germplasm interior quality comparation and assessment: mid-November then, in each residential quarter, take the Radix Isatidis plant, root division and leaf two parts, after routine is dried, measure, select Radix Isatidis adenosine content 6.0%～7.5%, alcohol extract content 25～33%, folium isatidis Content Determination of Indirubin 0.05%～0.06%, the germplasm of folium isatidis alcohol extract content 28%～31%;
Step 4, Radix Isatidis germplasm reproductive characteristic comparation and assessment: will remain basic former germplasm original place and keep, and survive the winter, and before in mid-March, the 2nd, each germplasm minute residential quarter was transplanted field planting, seeding row spacing 30 * 50cm, green separation net is set simultaneously, prevents mutually pollination between each germplasm; Observe their the Characters and stability separately, the selection plant height is 105～110cm, branch amount 6～12, the long 1.5～1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, the germplasm of thousand grain weigth 10～12g;
Step 5, breed and screen through continuous 2 years Radix Isatidis germplasm, final definite former germplasm morphological characters of advantage base: the long 29～32cm of main root, root diameter 0.9～1.1cm, the long 23～26cm of leaf, the wide 4～5cm of leaf, several 20～23 of single-strain blade, individual plant leaf dry weight 6～8g, individual plant root dry weight 10～13g, complete stool dry weight 17～20g; The biology of reproduction proterties: plant height is 105～110cm, branch amount 6～12, the long 1.5～1.8cm of fruit, the wide 0.35～0.4cm of fruit, the long 0.35～0.37cm of seed, the wide 0.1cm of seed, thousand grain weigth 10～12g; Interior quality: Radix Isatidis adenosine content 6.0%～7.5%, alcohol extract content 25%～33%, folium isatidis Content Determination of Indirubin 0.05%～0.06%, folium isatidis alcohol extract content 28%～31%.
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