CN106688592B - The screening technique of resistance to early ageing character during cotton breeding - Google Patents

The screening technique of resistance to early ageing character during cotton breeding Download PDF

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CN106688592B
CN106688592B CN201710043960.XA CN201710043960A CN106688592B CN 106688592 B CN106688592 B CN 106688592B CN 201710043960 A CN201710043960 A CN 201710043960A CN 106688592 B CN106688592 B CN 106688592B
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cotton
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early ageing
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高金权
王凤行
卢东琪
王妍卿
王连芬
田建全
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TIANJIN AGRICULTURAL TECHNOLOGY PROMOTION STATION
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Abstract

The present invention relates to a kind of screening technique of early ageing character resistance to during cotton breeding, implementation steps are as follows: (1) separation material for needing to screen being carried out southern breeding in Sanya, Hainan area by segregating population combination and planted;(2) it pours water when 50% or so cotton plant starts to budding primary, starts Controlled irrigation later;(3) during Controlled irrigation, starting material drought tolerance selection after material to be screened starts drought Stress responses is observed;(4) before cotton plant is shown in wadding, normal water management is kept, sees and stops pouring water immediately after wadding;(5) to middle and upper part fruit branch, segregating population can show to start to carry out Single-plant selection when obvious leaf function difference cotton plant blow-of-cottons;(6) low generation separation material is according to above-mentioned steps (1)~step (5) step sizing, until character is stablized.The present invention is mainly characterized by creating cotton plant early ageing occurrence condition by artificial induction, first is that improving the accuracy selected the material of resistance to early ageing;Second is that improving the efficiency of selection for the material of resistance to early ageing.

Description

The screening technique of resistance to early ageing character during cotton breeding
Technical field
The present invention relates to a kind of cotton variety selection, in particular to resistance to early ageing character during a kind of cotton breeding Screening technique.
Background technique
Early ageing seriously affects the yield and quality of cotton, and the slight normal underproduction 15% or so of early ageing, severe early ageing is then Can the underproduction 20%~50%, and fiber quality reduce.Currently, cotton premature senescence this general hair, breaking out and irreversible physiology venereal disease Evil, it is worldwide more and more common and serious, it has also become to restrict one of cotton yield, important restriction factor of stable yields.No With cotton variety premature senescence resistance characteristic, there is notable differences, therefore can pass through the cotton product of the resistance to early ageing of genetic breeding means breeding Kind.The dynamic Fluctuation studies have shown that cotton premature senescence that cotton variety early ageing occurs is occurred to have general hair, is broken out and irreversible The characteristics of, reply cotton premature senescence should be based on anti-in production, and the cotton variety of the resistance to early ageing of breeding is fundamentally to prevent cotton One of important measures of early ageing.
In cotton breeding practice, no matter line breeding or crossbreeding, it is all essential by from more huge Segregating population in filter out this critical process of the single plant with specific objective character.At present to early ageing character in breeding process Screening mainly observe cotton late growth stage cotton plant blade function activity situation, but appearance is compared in the performance of cotton premature senescence character It is influenced vulnerable to extraneous factor, different years and different tests intersite climatic environment, soil environment, field farming management level Equal many factors influence, and the performance of material early ageing character is often extremely unstable, seriously affect and interfere selection effect and selection effect Rate is generally difficult accurately to filter out the single plant of resistance to early ageing from segregating population.
Therefore, how more accurate from huge segregating generation group and efficient will have resistance to early ageing character Single plant screen, accelerate the cotton variety of resistance to early ageing breeding be problem urgently to be resolved in the work of current cotton breeding it One.
Summary of the invention
It is an object of the present invention to overcome the above deficiencies, provides a kind of sieve of resistance to early ageing character during cotton breeding Choosing method, can be accurate and efficient filters out from big segregating population from generation to generation with resistance to early ageing character cotton single plant.
The present invention using technical solution is to achieve the goals above: the sieve of resistance to early ageing character during a kind of cotton breeding Choosing method, it is characterised in that this method implementation steps are as follows:
(1) will need the low generation segregating population of separation material F2, F3, F4 that screens October early and middle ten days between by the end of October Period in, by segregating population combination in Sanya, Hainan area, i.e., 20 DEG C of the monthly mean temperature in long-term October to April in next year~ 30 DEG C, daily minimal tcmperature >=10 DEG C, illumination abundance;Long-term April in mid or late November to next year is dry season, and sand loam soil, preserve soil moisture energy Power is weak, and cotton field is poured water and is spaced 7 days~14 days by production is irrigated, and carries out southern breeding and plants, and group is planted in each segregating population combination Guarantee 800~1500 plants, segregating population plantation group higher from generation to generation should be larger;
(2) it pours water when 50% or so cotton plant starts to budding primary, using the cotton field of furrow irrigation, irrigation quantity is with the 2/3 of furrow Left and right is advisable;Using the cotton field of sprinkling irrigation, spray 20 minutes or so;Start Controlled irrigation later, control contains flower bud phase to first flower In stage phase, the soil relative water content of soil 0~40cm soil layer is 35% hereinafter, extensive after keeping arid stimulation 7 days~14 days It pours water again, cotton plant intuitively shows as leaf color deeply and shades, and blade wilting situation terminates dry when 20:00 or so cannot still restore Drought stimulation restores to pour water;To control drought stress degree, avoids excessively coercing and cause fruit abscission rate situation overweight and influence the later period Select effect;
(3) it during Controlled irrigation, observes after material to be screened starts drought Stress responses, blade fine day noon withers Listless, the drought-enduring material of the selection of starting material drought tolerance, generally this stage indicates later period resistance to early ageing;According to weather condition when operation, Fine day noon 11:00~14:00 and 15:00~19:00 in afternoon are observed twice;According to expert evidence blade noon wilt and Afternoon recovery situation difference performance, select the red rope label of more drought-enduring single plant system, each segregating population combination selects 200~ 300 single plants;Selection gist is that drought-enduring material wilting phenomenon evening occurs, and wilting in afternoon phenomenon restores early;
(4) cotton plant is shown in front of wadding, keeps normal water management, sees and stops pouring water immediately after wadding;
(5) to middle and upper part fruit branch, segregating population can show to start to carry out when obvious leaf function difference single cotton plant blow-of-cottons It selects good strains in the field for seed and selects;Single-plant selection standard are as follows:
A, it is selected in single plant from step (3) and selects;
B, Ye Gongneng is kept preferably in segregating population, and general complete stool falls off blade and yellow leaf number≤complete stool number of blade 25%;
C, 1~4 fruit branch of lower part, it is strong inside to enclose bell knot bell, and the first fruit section is at bell without missing, and the first and second fruit saves Boll probability >=75%;
D, 5~8 fruit branch of middle part, the first fruit saves Boll probability >=75%, and there can be no the inane fruit branches without knot bell;
E, there can be no the inane fruit branches without knot bell for 1~3 fruit branch of number from the top down at top;
The single plant for meeting above-mentioned five conditions of whole is selected, and be listed record main detection properties and characteristics, and single plant list is received, carried out Kind and fiber quality detection are copied, Single boll weight >=5.3g, single plant ginning outturn >=40%, upper half mean length >=28mm, fracture are selected Specific strength >=28cN/tex, mic value 3.7~5.2;The single plant that F2~F4 low generation filters out is by combination or same combination By the similar principle of character, single plant is classified and is mixed, next generation mixed planting continues to screen;
(6) low generation separation material is according to above-mentioned steps (1)~step (5) step sizing, until character is stablized;Continue to sieve The low generation material of choosing, the middle plantation group of step (1) select the mixed grain weight decision received according to the previous generation, expand plantation group as far as possible Body.
The beneficial effects of the present invention are:
(1) induction cotton plant later period early ageing character occurs under conditions of artificial controllable, forms early ageing character in segregating population Notable difference Differentiation, and continuously more can repeat to select from generation to generation, be improved while greatly improving efficiency of selection The accuracy of character determination.
(2) by Mutiple Choice constraint, rejecting does not have high yield, stable yields character, but the single plant of later period leaf better function Influence to selection accuracy and efficiency, further improves the accuracy and efficiency of selection of character determination.
(3) it is screened during south numerous added-generation, takes full advantage of numerous weather of the base winter drying without rain of South of Hainan Condition, can simplified condition, the economical and practical inductive condition for creating the generation of screening material later period early ageing character and otherness differentiation.
In short, the method for the present invention makes full use of natural conditions reverse thinking accuracy of judgement to screen, generate unexpected prominent Effect out has the features such as small investment, high-efficient, easy to operate, is widely used, application effect highly significant.
Specific embodiment
In conjunction with the preferred embodiment, specific embodiment, feature, method and step and the processing to providing according to the present invention Details are as follows for effect:
The screening technique of resistance to early ageing character, this method implementation steps are as follows during a kind of cotton breeding:
(1) will need the low generation segregating population of separation material F2, F3, F4 that screens October early and middle ten days between by the end of October Period in, by segregating population combination in Sanya, Hainan area, i.e., 20 DEG C of the monthly mean temperature in long-term October to April in next year~ 30 DEG C, daily minimal tcmperature >=10 DEG C, illumination abundance;Long-term April in mid or late November to next year is dry season, and sand loam soil, preserve soil moisture energy Power is weak, and cotton field is generally poured water and is spaced 7 days~14 days by production is irrigated, and carries out southern breeding and plants, and each segregating population combination is planted Group guarantees 800~1500 plants or so, and general segregating population plantation group higher from generation to generation should be larger.
(2) it pours water when 50% or so cotton plant starts to budding primary, using the cotton field of furrow irrigation, irrigation quantity is with the 2/3 of furrow Left and right is advisable;Using the cotton field of sprinkling irrigation, spray 20 minutes or so;Start Controlled irrigation later, control contains flower bud phase to first flower In stage phase, the soil relative water content of soil 0~40cm soil layer is 35% hereinafter, extensive after keeping arid stimulation 7 days~14 days It pours water again, cotton plant intuitively shows as leaf color deeply and shades, and blade wilting situation terminates dry when 20:00 or so cannot still restore Drought stimulation restores to pour water;To control drought stress degree, avoids excessively coercing and cause fruit abscission rate situation overweight and influence the later period Select effect.
(3) it during Controlled irrigation, observes after material to be screened starts drought Stress responses, blade fine day noon withers Listless, the drought-enduring material of the selection of starting material drought tolerance, generally this stage indicates later period resistance to early ageing;According to weather condition when operation, General fine day noon 11:00~14:00 or so and 15:00~19:00 in afternoon or so is observed twice;According to expert evidence leaf Piece noon wilts and the difference of recovery situation in afternoon shows, and selects the red rope label of more drought-enduring single plant system, each segregating population group Close 200~300 single plants of selection;Selection gist is that drought-enduring material wilting phenomenon evening occurs, and wilting in afternoon phenomenon restores early.
(4) cotton plant is shown in front of wadding, keeps normal water management, sees and stops pouring water immediately after wadding.
(5) to middle and upper part fruit branch, segregating population can show to start to carry out when obvious leaf function difference single cotton plant blow-of-cottons It selects good strains in the field for seed and selects;Single-plant selection standard are as follows:
A, it is selected in single plant from step (3) and selects;
B, Ye Gongneng is kept preferably in segregating population, and general complete stool falls off blade and yellow leaf number≤complete stool number of blade 25%;
C, 1~4 fruit branch of lower part, it is strong inside to enclose bell knot bell, and the first fruit section is at bell without missing, and the first and second fruit saves Boll probability >=75%;
D, 5~8 fruit branch of middle part, the first fruit saves Boll probability >=75%, and there can be no the inane fruit branches without knot bell;
E, there can be no the inane fruit branches without knot bell for 1~3 fruit branch of number from the top down at top;
The single plant for meeting above-mentioned five conditions of whole is selected, and be listed record main detection properties and characteristics, and single plant list is received, carried out Kind and fiber quality detection are copied, Single boll weight >=5.3g, single plant ginning outturn >=40%, upper half mean length >=28mm, fracture are selected Specific strength >=28cN/tex, mic value 3.7~5.2;The single plant that F2~F4 low generation filters out is by combination or same combination By the similar principle of character, single plant is classified and is mixed, next generation mixed planting continues to screen.
(6) low generation separation material is according to above-mentioned steps (1)~step (5) step sizing, until character is stablized;Continue to sieve The low generation material of choosing, the middle plantation group of step (1) select the mixed grain weight decision received according to the previous generation, expand plantation group as far as possible Body.
Using the administrative skill of removal leaf branch, other cotton fields in addition to the water management measure of aforementioned limitation are managed for cotton field management Reason measure is carried out according to normal cotton field high yield management measure.
To accelerate breeding work process, Cotton in China breeding work generally requires numerous in Sanya, Hainan area progress south.This Invention is planted to low generation (generally to F2, F3) separation material, in conjunction with the numerous work in south in China Sanya, Hainan area, and is required It is sowed in October early and middle ten days to the period between by the end of October, completion must be sowed at the beginning of 11 months the latest.First is that on the breeding time It can guarantee that cotton material can be harvested in early April normal mature, guarantee and being connected on the interior ground sowing time;Second is that guaranteeing In the dry season that a period of time of cotton material after buddingging before harvest is this area, and this area's retention capacity of soil It is weak, convenient for carrying out drought stress within the period of needs by pouring water, inducing cotton material early ageing occurrence condition, such as step (2) described.The present invention is making full use of Sanya, Hainan local climate environmental resource feature, in the numerous process in south of the normal procedure of breeding In carry out the work, operating method is simple, controllable, small investment, high efficiency.
The present invention is to improve selection accuracy and efficiency of selection, using Mutiple Choice technology: first is that containing flower bud phase to first flower Phase carries out the drought stress stage, the first reselection procedure is carried out according to the difference of material drought resisting sex expression, as described in step (3);Second is that In cotton plant blow-of-cottons to middle and upper part fruit branch stage, segregating population material can show to start carry out second when obvious leaf function difference Reselection procedure, as described in step (5).Through above-mentioned selection course, rejecting does not have high yield, stable yields character, knot bell difference or Cheng Lingjie Influence of the single plant of structure unevenness but later period leaf better function to selection accuracy and efficiency, to guarantee the accuracy and choosing of character determination Select efficiency.
The method of the present invention can refer to and answer in other climatic environments, the close area of resources supplIes and China's Sanya, Hainan area With.
The present invention is containing flower bud phase to the stimulation of flowering stage stage arid and cotton premature senescence with stronger positive correlation according to cotton Rule, contain flower bud phase to the stronger material of flowering stage stage drought-resistance ability, later period resistance to early ageing ability is generally relatively strong, therefore the present invention During the selection of cotton generation material, a period of time artificially is given to generation material to the flowering stage stage in Sheng flower bud phase and is done Drought stimulation, the early ageing of inducing cotton later period occur, according to material in Sheng Lei to flowering stage stage drought-enduring sex expression, bond material fertility Later period leaf function shows situation, and comprehensive other Breeding objective performances, is identified generation material and is screened;That is basis Early ageing trait expression difference in segregating population accurately and efficiently screens the single plant with resistance to early ageing character from segregating population Out.It is to create cotton plant early ageing occurrence condition by artificial induction in place of the main difference of the present invention and other breeding methods, First is that improving the accuracy selected the material of resistance to early ageing;Second is that improving the efficiency of selection for the material of resistance to early ageing.
Application example of the invention:
Winter in 2008 configures cross combination 30 in Sanya, Hainan;F1 combines spring in 2009 and plants in Tianjin, according to Observation, the identification of one Growing season, select 7 combinations;In mid-October, 2009, F2 separation material benefit the nation town kind in Sanya, Hainan It plants, 7 each combinations of combination plant 0.2 mu, and 1000 plants or so of group is planted in each combination.The screening of F2 generation material is according to preceding The progress of (2)~(5) step is stated, altogether 209 single plants of final election;209 single plants are divided into 5 single plant hybrid combinings by F3 generation, 2010~ 2011 annual Growing seasons continue to be planted in the Sanya, Hainan town that benefits the nation, and 5 combinations plant group 500 according to grain weight situation ~1500 plants or so, the selection of aforementioned (2)~(5) step, 423 single plants of final election;F4 is instead of afterwards through the selection of continuous plant, strain The procedures of breeding such as comparison, strain compare, comparision of production, select new lines 5 with resistance to early ageing character.
According to the method for the present invention, New cotton line 5 of the precocious resistance to suitable dense planting of early ageing of breeding, 2014, Efficiency in Buildings in Tianjin Area by Dry season influences, and cotton field early ageing situation is than more prominent, September 22nd, is investigated 5 strain early ageing indexes.Table 1 is 5 product It is early ageing index and seed cotton yield situation survey graft, 13~118,13~119,13~121,13~123,13~126 be application The resistance to early ageing new lines of the method for the present invention breeding.CK (Guo Xinmian 3) is production high yield kind.
Table 1 is the results show that the new lines early ageing index using this method breeding is lower, and seed cotton yield level is higher, table Reveal stronger resistance to early ageing characteristic.
1 new lines early ageing index of table and seed cotton yield situation survey graft
The present invention is directed in main conventional breeding methods at present to the selection of the resistance to early ageing character of cotton by place, weather, field Between many factors such as manage and influence, select that accuracy is poor, deficiency of low efficiency, providing one kind can be improved the resistance to early ageing character of cotton Select resistance to early ageing kind (strain) selection of cotton of accuracy and efficiency of selection.This method in cotton generation material by selecting During selecting, a period of time is given to generation material containing flower bud phase to two stages of flowering stage stage and cotton late growth stage Arid stimulation, human-induced cotton plant early ageing occurrence condition improve the accuracy and efficiency of selection of the selection of the cotton material of resistance to early ageing;Have Effect solves problem present in existing cotton breeding screening.
The above-mentioned detailed description carried out referring to embodiment to the screening technique of early ageing character resistance to during the cotton breeding is It is illustrative without being restrictive;Therefore the change and modification in the case where not departing from present general inventive concept, should belong to of the invention Within protection scope.

Claims (1)

1. the screening technique of resistance to early ageing character during a kind of cotton breeding, it is characterised in that this method implementation steps are as follows:
(1) by need the low generation segregating population of separation material F2, F3, F4 that screens October early and middle ten days between by the end of October when Between in section, by segregating population combination in Sanya, Hainan area, i.e., the monthly mean temperature 20 DEG C~30 in long-term October to April in next year DEG C, daily minimal tcmperature >=10 DEG C, illumination abundance;Long-term April in mid or late November to next year is dry season, sand loam soil, soil moisture conservation Weak, cotton field is poured water and is spaced 7 days~14 days by production is irrigated, and carries out southern breeding and plants, and each segregating population combination is planted group and protected 800~1500 plants of card, segregating population plantation group higher from generation to generation should be larger;
(2) it pours water when 50% or so cotton plant starts to budding primary, using the cotton field of furrow irrigation, irrigation quantity is with 2/3 or so of furrow It is advisable;Using the cotton field of sprinkling irrigation, spray 20 minutes or so;Start Controlled irrigation later, control contains flower bud phase to flowering stage rank Section, the soil relative water content of soil 0~40cm soil layer is 35% hereinafter, restoring to fill after keeping arid stimulation 7 days~14 days Water, cotton plant intuitively show as leaf color deeply and shade, and blade wilting situation terminates arid thorn when 20:00 or so cannot still restore Sharp recovery is poured water;To control drought stress degree, avoids excessively coercing and cause fruit abscission rate situation overweight and influence later period selection Effect;
(3) it during Controlled irrigation, observes after material to be screened starts drought Stress responses, blade fine day noon wilts, The drought-enduring material of the selection of starting material drought tolerance, generally this stage, indicates later period resistance to early ageing;It is fine according to weather condition when operation Its noon, 11:00~14:00 and 15:00~19:00 in afternoon were observed twice;According to expert evidence blade noon wilt and under The difference of noon recovery situation shows, and selects the red rope label of more drought-enduring single plant system, each segregating population combination selection 200~300 A single plant;Selection gist is that drought-enduring material wilting phenomenon evening occurs, and wilting in afternoon phenomenon restores early;
(4) cotton plant is shown in front of wadding, keeps normal water management, sees and stops pouring water immediately after wadding;
(5) to middle and upper part fruit branch, segregating population can show to start to carry out single plant choosing when obvious leaf function difference cotton plant blow-of-cottons It selects;Single-plant selection standard are as follows:
A, it is selected in single plant from step (3) and selects;
B, Ye Gongneng is kept preferably in segregating population, and general complete stool falls off blade and yellow leaf number≤complete stool number of blade 25%;
C, 1~4 fruit branch of lower part, it is strong inside to enclose bell knot bell, the first fruit section at bell without missing, and the first and second fruit section Boll probability >= 75%;
D, 5~8 fruit branch of middle part, the first fruit saves Boll probability >=75%, and there can be no the inane fruit branches without knot bell;
E, there can be no the inane fruit branches without knot bell for 1~3 fruit branch of number from the top down at top;
The single plant for meeting above-mentioned five conditions of whole is selected, and be listed record main detection properties and characteristics, and single plant list is received, carries out copying kind It is detected with fiber quality, selects Single boll weight >=5.3g, single plant ginning outturn >=40%, upper half mean length >=28mm, fracture is than strong Degree >=28cN/tex, mic value 3.7~5.2;The single plant that F2~F4 low generation filters out by combination or same combination in by property The similar principle of shape, single plant is classified and is mixed, and next generation mixed planting continues to screen;
(6) low generation separation material is according to above-mentioned steps (1)~step (5) step sizing, until character is stablized;Continue screening Low generation material, the middle plantation group of step (1) select the mixed grain weight decision received according to the previous generation, expand plantation group as far as possible.
CN201710043960.XA 2017-01-19 2017-01-19 The screening technique of resistance to early ageing character during cotton breeding Expired - Fee Related CN106688592B (en)

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CN112514759A (en) * 2020-11-30 2021-03-19 湖北省农业科学院经济作物研究所 Cotton thin planting, early maturing and high quality cultivation method
CN117789893B (en) * 2024-02-27 2024-04-30 河北省农林科学院农业信息与经济研究所 Breeding data prediction method based on correlation analysis

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CN104126389A (en) * 2014-07-22 2014-11-05 胡能兵 Method for selecting early-maturing yellow skin pepper germplasm
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CN1194089A (en) * 1998-04-07 1998-09-30 杭州常青保健食品有限公司 High temperature resistant mushroom and its breeding and cultivating method
CN101707963A (en) * 2009-12-30 2010-05-19 河南省农业科学院 Cotton breeding method
CN102206672A (en) * 2011-04-01 2011-10-05 南京农业大学 Molecular breeding method for improving biological yield of cotton
CN102498847A (en) * 2011-10-19 2012-06-20 南京农业大学 Screening method of advantage-based stock seed of isatis root
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CN104126389A (en) * 2014-07-22 2014-11-05 胡能兵 Method for selecting early-maturing yellow skin pepper germplasm
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