CN103262791B - Selective breeding method of good quality seed of polygonum multiflorum - Google Patents
Selective breeding method of good quality seed of polygonum multiflorum Download PDFInfo
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- CN103262791B CN103262791B CN201310236420.5A CN201310236420A CN103262791B CN 103262791 B CN103262791 B CN 103262791B CN 201310236420 A CN201310236420 A CN 201310236420A CN 103262791 B CN103262791 B CN 103262791B
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Abstract
The invention provides a selective breeding method of good quality seeds of polygonum multiflorum. The selective breeding method comprises the following steps of: screening variety variation according to shapes, colors and luster of leaves on the aspect of selecting the high-quality seeds; and investigating individual differences of single plants from three factors including the shape and the weight of a tuber and the content of a main active component of the tuber. According to the selective breeding method disclosed by the invention, the screening efficiency of the good quality seeds is higher than that in the conventional single-factor screening investigation so as to be good for ensuring a stable inheritable character strain; the good quality seeds obtained by using the selective breeding method of the improved variety are increased by about 200% when being compared with cultivated wild breeder seeds; and the selective breeding method of the good quality seeds, disclosed by the invention, is strong in operability and simple, and is suitable for large-scale implementation.
Description
Technical field
The present invention relates to the selection that a kind of traditional Chinese medicine implantation methods relates to a kind of fleece-flower root breeding in particular.
Technical background
The fleece-flower root (Polygonum muhiflorum Thunb.) is the perennial voluble herb plant of polygonaceae, its block root is the famous and precious large traditional Chinese medicine of China, the fleece-flower root has effect of liver kidney, benefiting essence-blood, black beard and hair, strengthening the bones and muscles, be nourishing liver and kidney conventional Chinese medicine, also can be used for arthralgia due to wind-dampness and sore rash itch.Research in recent years finds, the fleece-flower root anti-ageing, strengthen immunity of organism, antibacterial, blood and improve in cardiovascular function etc. and have many distinctive features to cause people's extensive concern.The vine of multiflower knotweed, can people's medicine also known as Polygonum multiflower knotweed.The fleece-flower root is mainly distributed in Guangxi, Guangdong, Guizhou, Sichuan, Hubei, Jiangsu, Henan, and in addition, in Yunnan, also there is distribution on Shaanxi, Hunan, Jiangxi, Zhejiang, Fujian, Shandong, the ground such as Hebei.Fleece-flower root distribution is very wide, and each place of production is various in style, and quality is uneven, and the fleece-flower root has a kind of substitute species to be radix cynanchi bungei, the block root that radix cynanchi bungei (Cynanchum bungei Decne.) is dicotyledon medicine Asclepiadaceae plant Cynanchum bungei Decne; The adulterant easily obscured even is pretended to be the fleece-flower root by some places.Therefore, the classification of fleece-flower root wild resource kind, qualification, quality analysis and fine-variety breeding are important element tasks.
The Leaf pattern proterties to the fleece-flower root 31 germ plasm resources in main area in the whole nation such as Wei Yanmei measures, and analyzes the genetic variation of Different sources fleece-flower root blade.Find for each individual plant leaf proterties in examination fleece-flower root provenance comparatively stable, variation is mainly derived from the indexs such as wide in SL A, blade, Ye Kuan; Between provenance, each character variation of leaf is obvious, and variation is mainly derived from the indexs such as wide in single leaf dry weight, blade area, blade, leaf is long.Dependency relation research between each proterties of tuber fleeceflower leaf is shown, SL A and single leaf dry weight are all long with leaf, wide in blade, Ye Kuan, leaf are thick, petiole is long in extremely significantly positive correlation, and with WWR (ratio of the wide 1/2 place's width long with leaf of leaf), the LWR ratio of the long 1/2 place's width of leaf (the leaf length with) in remarkable negative correlation, this siphonal lobe is long, wide in blade, Ye Kuan, petiole long these intuitively leaf characteristic index each other in extremely significantly positive correlation.Principal component analysis result shows, the cumulative proportion in ANOVA of front 3 principal components, up to 97.4%, more accurately can reflect the general performance of fleece-flower root provenance leaf proterties.Cluster analysis result shows, is three major types group for examination 31 fleece-flower root Species source divisions.1st monoid geographic area is Central Guizhou, western part and the adjacent northwestward, Guangxi and High Altitude in West China area; 2nd monoid geographic area is the most area in Hunan, Hubei, Sichuan, Guangdong and Guangxi; 3rd monoid geographic area is Anhui, Jiangsu, Henan and Shandong.The cluster analysis demonstrating leaf proterties shows that the close provenance in geographical position can be got together.This research is that the assortment of the fleece-flower root provides certain foundation.
Hu Xiuyue etc. are guidance by plant fine-variety breeding and the technology of breeding, and the basis of forefathers' summary of experience is carried out.By extensively collecting fleece-flower root kind matter, build up field gene bank; Medicinal material is just planted, Stibene-glucoside, total starches, Anthraquinones, lecithin content are evaluation of medical materials' quality index in employing, by the breeding appraisement system that the economical characters such as quality of medicinal material and growth adaptability, output, ripe phase are index, collected kind of matter is evaluated, filters out elite germplasm; Carry out seed growing, the raising technology research of the fleece-flower root system such as cutting propagation, tissue cultures are bred, and formulate raising technology code and seedling quality requirement.But all there is setting parameter complexity in the index of above-mentioned fine-variety breeding, the problem such as operation easier is large aborning.
Summary of the invention
For solving the problem, the invention provides a kind of fleece-flower root fine-variety breeding method, wherein, the factor that high-quality breeding of the present invention is investigated is: blade shape and the color and luster of 1) investigating standard as kind genetic variation proterties, have about SL A in the prior art, wide in blade, the indexs such as Ye Kuan investigate the research report of fleece-flower root regionality variation, the present invention just selects from the shape of blade and the kind of color and luster two factor pair fleece-flower root kinds, reason is: specifically quantize SL A, wide in blade, the indexs such as Ye Kuan are difficult to realize in the large-scale production of reality, simultaneously owing to planting the illumination in plot, the difference of water and fertilizer condition, also institute's These parameters may be caused to change, and judge intuitively from leaf morphology, method is simple, workable, also prove that by rejecting leaf morphology be narrow avette individual plant in practice, just can effectively carry out preferably from provenance, 2) investigate individual plant Tuber Size and the form of standard as kind per unit area yield, investigate according to per unit area yield and active component investigation, find that elongated cylindrical is more excellent than spindle, investigated data according to 5 years in addition to find, the fleece-flower root kind of elongated cylindrical rhizome also has high-quality in genetic stability, 3) content of the main active Stibene-glucoside of standard is investigated as medicinal material medical value, the active component of polygonum multiflorum medicinal material is a lot, but Stibene-glucoside is generally acknowledged main active, can simplify detection difficulty greatly.Particular content of the present invention is as follows:
A kind of fleece-flower root fine-variety breeding method is blade shape color, the shape size quality of rhizome, main active content three indexs to the index of high-quality Single-plant selection in fine-variety breeding.
Described fine-variety breeding method comprises the following steps:
1) gather season in existing polygonum multiflorum planting land for growing field crops, select high-quality individual plant, the alternative condition of high-quality individual plant is: leaf is heart-shaped, bottle green; Block root is elongated cylindrical, and block root table face is flawless, individual plant block root weight >=200g, individual plant block radical >=3; In block root, Stibene-glucoside content is greater than 1.5%;
2) next year is by step 1) in the stolon of individual plant selected and subterranean stem be used for seedling breeding, high-quality individual plant concentrates cuttage to plant, planting conditions and fleece-flower root field growth conditions indifference, collecting season selects high-quality individual plant again, alternative condition and step 1) identical;
3) repeat step 2) described in selection high-quality individual plant 2-5 time, until step 1) described alternative condition proterties is stable, Group Consistency reaches more than 90%, obtains fleece-flower root breeding.
Step 2) described in stolon and subterranean stem be: thick more than 2mm, grows vigorous, without damage by disease and insect, the stem of biennial band fibrous root.
Step 2) described in cuttage plantation for saving with secateurs clip tool 2 ~ 3, the stem branch of long 15 ~ 20cm does cuttage seeding.
Advantageous Effects of the present invention is: 1) the present invention screens from blade shape color and luster qualitative variation in the selection of high-quality breeding, the individual difference of individual plant has been investigated from content three factors of tuber shape, weight and main active, the single factor test screening that the screening effeciency of breeding of the present invention is more conventional is investigated higher, is conducive to determining stable genetic character strain;
2) breeding that fine-variety breeding method of the present invention obtains hands over the wild ancestor volume increase of existing plantation close to 200%;
3) the invention provides fine-variety breeding method workable, method is simple, is applicable to scale and implements.
Embodiment
The region that the compliance test result of method of the present invention is selected is dan post county, Chongqing, this area is positioned at Chongqing, Hubei, Hunan border land, the genetic distance of Radix Polygoni Multiflori germplasm resource out of office has the speciality of intermediate altitude monoid and High aititude monoid concurrently, and this area's polygonum multiflorum planting is huge, it is one of main producing region of the fleece-flower root.Concrete method validation research contents is as follows:
(1) fine individual plant mixing is selected
In November, 2003, land for growing field crops selection is carried out, the large Tanaka of wild cultivated population to the wild fleece-flower root resource of stone column, aerial growth gesture is chosen to the individuality of natural differentiation and microcommunity strong, underground part piecemeal root is sturdy, flawless, the individual plant that the Characters is in spite of illness unexcellent.Main Single-plant selection proterties is: leaf is heart-shaped, and bottle green, block root is elongated cylindrical, and block root table face is flawless, individual plant block root weight >=200g, individual plant block radical >=3.Combined block root Stilbene Glucoside assay result, selects the individual plant that Stibene-glucoside content is greater than 1.5% simultaneously.Qualified individual plant is decided to be primary election individual plant, amounts to and select individual plant 1000 strain.In 11 ~ December, when the fleece-flower root is gathered, the rhizome of selected individual plant is used for seedling breeding.Choose thick more than 2mm, grow vigorous, without damage by disease and insect, biennial band fibrous root rhizome.With secateurs clip tool 2 ~ 3 joint, the stem branch of long 15 ~ 20cm does cuttage seeding.Seedling is carried out field-transplanting at stone column Yue Lai town monastery Ping Cun by mid-March, 2004.
In November, 2005, from individual plant colony selected by 2004, select leaf heart-shaped, bottle green, block root is cylindrical, and block root table face is flawless, individual plant block root weight >=200g, individual plant block radical >=3, the biennial fleece-flower root individual plant that Stibene-glucoside content is greater than 1.5%.The underground rhizome of selected individual plant is used for seedling breeding.At the beginning of 2006 4 months, the seedling of individual plant selected by 2005 is carried out field-transplanting, in mid-November, 2007 continues by criterion picks fleece-flower root individual plant 3000 strain before from transplanting colony.
(2) variety comparative test
2008 ~ 2009 high by the output optimized through 5 years, and individual plant colony and the wild fleece-flower root of Stibene-glucoside stable content carry out variety comparative test, proves output performance and the stability of seed selection individual plant colony further.Name at the beginning of the new varieties of seed selection from the wild fleece-flower root as " No. 1, the fleece-flower root ".Variety comparative test result shows, the wilder fleece-flower root volume increase of the fleece-flower root No. 1 average yield 215.15%, four community volume increase show as pole significant difference (table 1).Compared with the wild fleece-flower root, selected " No. 1, the fleece-flower root " block root is longer, more sturdy, and block root shape is more consistent, in elongated cylindrical; Stem on fleece-flower root rattan is sturdy, and aerial growth is vigorous, and blade is comparatively large, and blade profile is heart-shaped (table 2, table 3).Through 5 years, the preferred fleece-flower root No. 1 strain shape difference was little, and comparatively neat and consistent, output is high, steady quality.
Table 1 2008-2009 fleece-flower root varietal yield test yield result (fresh weight)
Table 2 2008-2009 fleece-flower root varietal yield test economic characters copy kind of a result
Table 3 2008-2009 fleece-flower root varietal yield test biological character copies kind of a result
(3) regional test
2010 ~ 2011: participate in stone column county fleece-flower root strain's area experiment.Regional test result shows, " No. 1, the fleece-flower root " three some average yield per mus 1252.2 jin, and than the wild fleece-flower root volume increase of contrast 210.5% (table 5), Analysis of variance, difference reaches pole significance level.Each pilot average per unit area yield Yi Yue Lai town is the highest, and being 1344.4kg/ mu, takes second place in three beneficial townshiies, and be 1209.1kg/ mu, stone local is minimum, is 1200.0kg/ mu (table 4).And three points all increase production, difference is extremely remarkable.Performance of cultivar is strong adaptability, it is prosperous to grow, output is high, block radical is many.Block root is irregular elongated cylindrical, sturdy hypertrophy.
Table 4 2010 ~ 2011 years fleece-flower root new varieties regional test results
Note: planting density 4500 plants/acre.
Table 5 three test site fleece-flower root output statistics results (unit: kilogram)
(4) production test
Within 2010, production test is carried out in Yue Lai town, three Yi Xiang, stone local, 4, Xia Lu town, and each some cultivated area 2 mu, about mu plants 4500 strains.Within 2011, survey and produce, result is as following table.The each point fleece-flower root No. 1 average yield of participating in the experiment is 1293.5kg/ mu, wild fleece-flower root average yield is 439.5kg/ mu, No. 1, the kind fleece-flower root of new seed selection comparatively contrasts volume increase 194.3%, show as high yield, stable yields, happiness is fertile, and adaptability is wider, best in quality, variety stability is good, and per mu yield value is high, deeply welcomes by peasant.
No. 1, table 7 fleece-flower root and wild fleece-flower root Yield compari@
Note: planting density 4500 plants/acre.
Claims (3)
1. a fleece-flower root fine-variety breeding method, is characterized in that: be blade shape color, the shape size quality of rhizome, main active content three indexs to the index of high-quality Single-plant selection in fine-variety breeding;
Described fine-variety breeding method comprises the following steps:
1) gather season in existing polygonum multiflorum planting land for growing field crops, select high-quality individual plant, the alternative condition of high-quality individual plant is: leaf is heart-shaped, bottle green; Block root is elongated cylindrical, and block root table face is flawless, individual plant block root weight >=200g, individual plant block radical >=3; In block root, Stibene-glucoside content is greater than 1.5%;
2) stolon of the individual plant selected in step 1) and subterranean stem are used for seedling breeding by next year, high-quality individual plant concentrates cuttage to plant, planting conditions and fleece-flower root field growth conditions indifference, collecting season selects high-quality individual plant again, and alternative condition is identical with step 1);
3) repeat step 2) described in selection high-quality individual plant 2-5 time, until alternative condition proterties described in step 1) is stable, Group Consistency reaches more than 90%, obtains fleece-flower root breeding.
2. fleece-flower root fine-variety breeding method according to claim 1, is characterized in that: step 2) described in stolon and subterranean stem be: thick more than 2mm, grows vigorous, without damage by disease and insect, the stem of biennial band fibrous root.
3. fleece-flower root fine-variety breeding method according to claim 1, is characterized in that: step 2) described in cuttage plantation for saving with secateurs clip tool 2 ~ 3, the stem branch of long 15 ~ 20cm does cuttage seeding.
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| CN105284605B (en) * | 2015-10-19 | 2018-02-02 | 重庆市中药研究院 | A kind of radix scrophulariae breeding of new variety method applied to southwest |
| CN105532464A (en) * | 2016-01-09 | 2016-05-04 | 佛山市金蓝领教育科技有限公司 | Fleece flower root browning-prevention induction medium and preparing method thereof |
| CN107278593A (en) * | 2017-06-22 | 2017-10-24 | 十堰市农业科学院 | A kind of method for culturing seedlings for promoting Caulis Polygoni Multiflori cuttage high density to survive |
| CN112535101B (en) * | 2020-12-02 | 2022-05-31 | 太极集团重庆涪陵制药厂有限公司 | Perilla frutescens fine variety breeding method |
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| CN1934933A (en) * | 2006-10-08 | 2007-03-28 | 南京林业大学 | Tissue culture quick-breeding method for polygonum multiflorum |
| CN102960164A (en) * | 2012-11-28 | 2013-03-13 | 贵州信邦制药股份有限公司 | Method for planting polygonum multiflorum |
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| CN1934933A (en) * | 2006-10-08 | 2007-03-28 | 南京林业大学 | Tissue culture quick-breeding method for polygonum multiflorum |
| CN102960164A (en) * | 2012-11-28 | 2013-03-13 | 贵州信邦制药股份有限公司 | Method for planting polygonum multiflorum |
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