CN104160843B - Wild salviae miltiorrhizae seedling simulation propagation method - Google Patents

Wild salviae miltiorrhizae seedling simulation propagation method Download PDF

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CN104160843B
CN104160843B CN201410260624.7A CN201410260624A CN104160843B CN 104160843 B CN104160843 B CN 104160843B CN 201410260624 A CN201410260624 A CN 201410260624A CN 104160843 B CN104160843 B CN 104160843B
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salviae miltiorrhizae
seedling
radix salviae
wild
simulation
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CN104160843A (en
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袁勋
麻兵继
黄勇
包戈
王世峰
曹金斌
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Nanyang Boruikang Technologies Co Ltd
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Abstract

The invention relates to a seedling propagation technology, in particular to a wild salviae miltiorrhizae seedling simulation propagation method. The wild salviae miltiorrhizae seedling simulation propagation method includes the steps of selection and breeding of good wild salviae miltiorrhizae seedlings, secondary selection and breeding of single good wild salviae miltiorrhizae seedlings, nurture in a plant-to-row garden and a nurture garden, building of a propagation foundation of a simulated wild salviae miltiorrhizae seedling simulation environment, soil preparation, use of organic fertilizer, sowing, building of a simulated wild salviae miltiorrhizae propagation and growth environment, and field management. The wild salviae miltiorrhizae seedlings have the advantages of being good in disease resistance, high in yield and content of effective elements, and the like, most medicinal herb growers of a genuine producing area go up to mountains for gathering wild salviae miltiorrhizae seedling roots and wild salviae miltiorrhizae seedling seeds in the prior art, mountains are destroyed, and water and soil losses are caused. The wild salviae miltiorrhizae seedling simulation propagation technology can well solve the problems that the wild salviae miltiorrhizae seedlings have a lot of plant diseases and insect pests and the quality and yield of the wild salviae miltiorrhizae seedlings are reduced, the quality of the salviae miltiorrhizae seedlings is ensured, the yield and quality of the salviae miltiorrhizae are improved, and meanwhile the ecological environment is protected.

Description

The propagation method of simulation wild Radix Salviae Miltiorrhizae seedling
Technical field
The present invention relates to the reproduction technique of a kind of seedling, be specifically related to a kind of propagation method simulating wild Radix Salviae Miltiorrhizae seedling.
Background technology
Mahjong, the abundant state of ancient title, abounds with Radix Salviae Miltiorrhizae, because of best in quality, evident in efficacy, and titled with the abundant Radix Salviae Miltiorrhizae of place name.Abundant Radix Salviae Miltiorrhizae Come from Gu, Xing Yujin, be contained in bright clearly, spread so far: " Radix Salviae Miltiorrhizae king, abundant governor;Quality better, curative effect is good;Shanghai, the medicine village, Wuhan Hide;Travel by water, walk to study abroad ... " the good reputation of ballad.Beam TAO Hong-Jing " Mingyi Bielu " carries: " the raw Tongbai Shan Mountain Semen Coicis of Radix Salviae Miltiorrhizae, Tongbai Mountain is the mountain risen in Huasui River, and the Tongbai in the non-east of a river is also ... ", mahjong Radix Salviae Miltiorrhizae likeness in form Radix Ginseng, and color of the leather is red red as red, medical material quality Hard close, the wooden core of skin depth is thin, and fiber is few, fractures in radioactivity Flos Chrysanthemi shape.Through Chinese Medicine academy of science, Nanyang medicine inspecting institute, wide White Cloud Mountain, state Chinese medicine company limited of Hutchison China Trade Holdings, Beijing University of Chinese Medicine, Zhengzhou University, Agricultural University Of He'nan, Henan science and technology are greatly Learn chemical examination: tanshinone Ⅰ content is 1.182%, cryptotanshinone is 1.9235%, tanshinone IIA is 0.6503%, salvianolic acid is 9.9214%, it is 3 times of States Pharmacopoeia specifications standard.Therefore 2003 by national quality technical supervision inspection and quarantine regard as " Radix Salviae Miltiorrhizae originate in Ground ".
In recent years, along with Radix Salviae Miltiorrhizae Chinese crude drug demand is continuously increased by people, wild Chinese crude drug can not meet the mankind Demand to high-quality genuine medicinal materials, and artificial extensive single cropping causes ecological environment unbalance, easily causes multiple pest and disease damage Occurring, environmental pollution causes harmful substance and heavy metal severe overweight etc. in medical material, and these have had a strong impact on medicinal plants product Quality and quality, Application to the Chinese medical herbs has special strict demand in medicinal industry, its product quality.Therefore, simulation open country is implemented Raw Radix Salviae Miltiorrhizae production technology, it is achieved green ecological agricultural, organically combines the advantage of Radix Salviae Miltiorrhizae Chinese crude drug field production and wild collection Together, quality of medicinal material difference and resource, environmental destruction, medical material plantation and grain kind that current Producing medicinal herbs faces are solved Plant the four problems striving ground.Anxious ecological environmental protection to be achieved, resource regeneration, comprehensive utilization, farmland saving, high-quality genuine medicinal materials The technical problem produced.
Summary of the invention
It is an object of the invention to overcome deficiency of the prior art and provide the one can farmland saving, it is provided that Yi Zhongan Complete effective, steady quality, the reproduction technique of green simulation wild Radix Salviae Miltiorrhizae seedling.
The object of the present invention is achieved like this:
A kind of propagation method simulating wild Radix Salviae Miltiorrhizae seedling, comprises the following steps that
1) excellent wild Radix Salviae Miltiorrhizae kind is selected good strains in the field for seed and is educated: select blade profile, flower pattern, on the ground cane and underground rhizome face at Radix Salviae Miltiorrhizae Proterozoic Color, the Radix Salviae Miltiorrhizae that plant growing form is consistent, select to infect without pest and disease damage, tanshinone IIA content >=0.4%, and content of danshinolic acid B >= The wild good species strain of 7%, sets up simulation wild Radix Salviae Miltiorrhizae and fosters garden, carry out fostering observation;
2) the secondary individual plant selection of wild good species strain: in the simulation wild Radix Salviae Miltiorrhizae set up fosters garden, selection meets former The feature of wild Radix Salviae Miltiorrhizae kind, characteristic, kind strain be healthy and strong, best in quality, fine individual plant without pest and disease damage, and tanshinone IIA content >= 0.4%, content of danshinolic acid B >=7% aspect carries out secondary individual plant selection, again selected individual plant is migrated to plant garden and fosters garden to comfort Educate;
3) foster garden to foster: to carry out the fine individual plant in fostering garden classifying, transplanting, carry out grain weight feature mirror Not, the good salvia plant seed list preferably gone out being received single Tibetan, numbers in order, as the material of selection-breeding new varieties, entrance is comforted The garden of educating is fostered;
4) simulation wild Radix Salviae Miltiorrhizae seedling breeding base is set up: simulation wild Radix Salviae Miltiorrhizae seedling breeding base selects radius around Without appointing river polluter in 5km, air quality meets GB3095-1996 first quality standard, and height above sea level is 250m 400m, from So vegetation 40%-60%, the gradient is 10-20 degree, and nursery stage precipitation mid-July mid-June should account for 20% left side of annual precipitation The right side, temperature is more than 30 DEG C, and light application time is more than 9 hours, and shading rate≤65-70% in after planting 11 days, irrigation water meets GB5084-92 first quality standard, soil is sandy red, yellow soil, meets GB15618-1995 quality standard;
5) use with fertilizer: before whole ground, use that health of becoming thoroughly decomposed is organic has a fertile 112.5kg-450kg, all for every 1 hectare wholely Even spreads fertilizer over the fields on the ground;Preferably ploughing shallowly in simulation wild Radix Salviae Miltiorrhizae seedling breeding base, within tilth is 20cm, then ploughs plain grinding thin; The simulation every 300cm in wild Radix Salviae Miltiorrhizae seedling breeding base plays high furrow, and high furrow clear span is 270cm, and then furrow width 30cm harrows the most whole Flat;
6) sowing: the sowing time selects early July mid-June to be the Radix Salviae Miltiorrhizae sowing time, and every 667 application rates are 2.5kg, before type of seeding sowing, adds simulation wild Radix Salviae Miltiorrhizae seedling breeding base fine earth 7.5kg and mixes in every 2.5kg salvia seeds Even, it is sprinkling upon uniformly in the soil on high furrow, then rolls with the waddy of a diameter 20cm and suppress, it is also possible to Hsinchu besom Pat the seed spreading in soil, make salvia seeds be fully contacted with soil;
7) set up simulation wild Radix Salviae Miltiorrhizae and breed growing environment:
(1) play staking out: every 2.5m plays the little staking out of a Φ 4-7cm high for 35cm in the furrow of base, horizontal on furrow take One root length 3.1m, the bamboo stem of Φ 3cm-5cm, and by bamboo stem tightly tie up in little staking out, bamboo stem is away from ground 30cm;
(2) cover net: select shading rate 65-70%'s to herd with sunshade net, and will herd and ride over uniformly on bamboo stem with sunshade net, In staking out, shaded effects are affected with Anti-blow-open with sunshade net tying up to tightly by herding with rope;
(3) water: above the sprinkling irrigation all handed over by water with irrigation sprinkler to sunshade net, by the aperture of sunshade net, make water slowly Fall on nursery furrow;
(4) temperature and relative temperature in net: before emerging, relative humidity is maintained at more than 25 DEG C, and relative humidity should be maintained at 75 On, start to emerge after 5 days, in 9 days, Radix Salviae Miltiorrhizae Seedling all sprouts substantially;
(5) take off net to put away sports equipment: Seedling goes out 5-7 days to take off net puts away sports equipment, and takes off net and puts away sports equipment and should carry out in the afternoon, should not be at point in the morning 10 Net is taken off, in case day drinks wound at 3 in afternoon;
8) field management: should pull out the various weeds in field after taking off net in time, must not use chemical herbicide, simulation open country Giving birth to Radix Salviae Miltiorrhizae seedling breeding base, early stage answers relative humidity higher, and soil is the most dry just should water in time, and the later stage should before Seedling goes out garden Suitably control water, when running into rainfall excess weather, the water in simulation wild Radix Salviae Miltiorrhizae seedling breeding base should be discharged in time, should anti-root-rot Sick generation.
Based on the above, in simulation wild Radix Salviae Miltiorrhizae sapling multiplication growing environment, nursery base furrow width 300cm, uses The sanitary cleaning bamboo stem of 320cm length and the little timber of 50cm length are support sunshade net support, sunshade net overhead 25-30cm, use Online sprinkling irrigation, makes nursery base relative humidity should be maintained at 55%-60%, within 5-7 days, emerges, after in 10 days, Seedling all sprouts, and in the afternoon 4 Sunshade net is removed after Dian.
Present invention have the advantage that:
1, economic benefit
The Radix Salviae Miltiorrhizae seedling that simulation wild Radix Salviae Miltiorrhizae seedling breeding technology of gathering produces, can provide 15-20 mu by every mu of seedling field Land for growing field crops uses, and every mu of land for growing field crops can increase production 90Kg, 18 yuan/Kg of existing market valency, and therefore 10000 mu of lands for growing field crops can increase economic benefit 16200000;Simultaneously, moreover it is possible to reduce labour force and put into.
2, social benefit
Wild Radix Salviae Miltiorrhizae seedling is good because of its disease resistance, yield and active constituent content advantages of higher, the medicine of tradition Genuine producing area More agriculture is gone up a hill and is excavated wild Radix Salviae Miltiorrhizae kind root and seed, damage hill, causes soil erosion.Simulation wild Radix Salviae Miltiorrhizae seedling breeding technology Can be good at solving that Radix Salviae Miltiorrhizae seedling pest and disease damage is many, quality degradation, the problem such as yield poorly, it is ensured that the quality of Radix Salviae Miltiorrhizae seedling, Improve Radix Salviae Miltiorrhizae yield and quality, protect ecological environment simultaneously.
Accompanying drawing explanation
Fig. 1 is different repetition salvia seeds mass of 1000 kernel.
Fig. 2 is the comparison of the fresh weight of three different fertilities nursery and dynamically changes.
Fig. 3 is the comparison of three different fertilities nursery dry weights and dynamically changes.
Fig. 4 is the comparison of three different fertilities nursery plant heights and dynamically changes.
Fig. 5 is comparison that the stem of three different fertilities nursery is thick and dynamically changes.
Fig. 6 is the comparison of the root bar number of three different fertilities nursery and dynamically changes.
Fig. 7 is the comparison of the fibrous root number of three different fertilities nursery and dynamically changes.
Fig. 8 is the comparison of the seedling vigorous index of three different fertilities nursery and dynamically changes.
Fig. 9 is the comparison of the fresh weight of three different seeding quantity nursery and dynamically changes.
Figure 10 is the comparison of three different seeding quantity nursery dry weights and dynamically changes.
Figure 11 is the comparison of three different seeding quantity nursery plant heights and dynamically changes.
Figure 12 is comparison that three different seeding quantity nursery stems are thick and dynamically changes.
Figure 13 is the comparison of the root bar number of three different seeding quantity nursery and dynamically changes.
Figure 14 is the comparison of three different seeding quantity nursery fibrous root numbers and dynamically changes.
Figure 15 is the comparison of three different seeding quantity seedling raising and strengthening indexes and dynamically changes.
Figure 16 is the different seedling raise period impacts on abundant Radix Salviae Miltiorrhizae Seedling Height.
The impact on abundant Radix Salviae Miltiorrhizae Seedling leaf area per plant of Figure 17 difference seedling raise period.
Figure 18 is different seedling raise period seedling leaves Growth trends changes.
Figure 19 is different seedling raising manners Growth of blade changes.
Figure 20 be difference shade natural law seedling fresh weight change.
Figure 21 be difference shelter from heat or light mode seedling dry weight change.
Figure 22 be difference shade natural law Seedling Height change.
Figure 23 is that three differences are shaded the thick comparison of natural law nursery stem and dynamically changing.
Figure 24 is that three differences are shaded the comparison of natural law nursery root bar number and dynamically changing.
Figure 25 is that three differences are shaded the comparison of fibrous root number of natural law nursery and dynamically changing.
Figure 26 is that three differences are shaded the comparison of seedling vigorous index of natural law nursery and dynamically changing.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
A kind of propagation method simulating wild Radix Salviae Miltiorrhizae seedling, comprises the following steps that
First, cultivate the excellent genetic factor of wild Radix Salviae Miltiorrhizae
Chinese crude drug elite germplasm, is the excellent gene Phenotypes that are integrated on the former phytobiocoenose of a kind of Chinese crude drug, tool Having the relative uniformity on relative heritability and major traits, quality of medicinal material and yield are played conclusive by the quality planting matter Effect.First have to pay attention to the cultivation of improved seeds, as without excellent kind matter, even if having preferable natural environment and cultivation technique, Also cannot change economic characters and the curative effect of its original, good " As a man sows, so he shall reap " that common saying is said, this is simple and vivid Language, the scientific meaning that rich top Piao is not broken.Therefore, cultivate good salvia genetic factor, be that enforcement simulation wild Radix Salviae Miltiorrhizae is green The key of color production of crude drugs.
Any improved seeds all should often roguing pure keeping, take measures in time to prevent variety deterioration.The variet complexity such as otherwise Purificating and rejuvenating after degenerating seriously, that just gets twice the result with half the effort again.Should take appropriate measures according to the reason of variety hybriding degradation.
1) excellent wild Radix Salviae Miltiorrhizae kind is selected good strains in the field for seed and is educated: select blade profile, flower pattern, on the ground cane and underground rhizome face at Radix Salviae Miltiorrhizae Proterozoic Color, the Radix Salviae Miltiorrhizae that plant growing form is consistent, select to infect without pest and disease damage, tanshinone IIA content >=0.4%, and content of danshinolic acid B >= The wild good species strain of 7%, sets up simulation wild Radix Salviae Miltiorrhizae and fosters garden, carry out fostering observation;
2) the secondary individual plant selection of wild good species strain: in the simulation wild Radix Salviae Miltiorrhizae set up fosters garden, selection meets former The feature of wild Radix Salviae Miltiorrhizae kind, characteristic, kind strain be healthy and strong, best in quality, fine individual plant without pest and disease damage, at tanshinone IIA content >=0.4%, under the conditions of content of danshinolic acid B >=7%, carry out secondary individual plant selection, again selected individual plant is migrated to plant garden and comforts The garden of educating is fostered;
3) garden is fostered to foster: to carry out the fine individual plant in fostering garden classifying, transplanting, carry out feature discriminating, especially It is thousand grain weigth, can be as weighing seed quality and the reference index of Maturity, to the good salvia plant preferably gone out Single Tibetan received by seed list, numbers in order, and as the material of selection-breeding new varieties, enters and fosters garden to foster, in order to more convenient enters Row controlled observation and the comparison of quality and yield, it is also possible to carry out the setting of plot area, plot area 5m2, row, spacing in the rows be 40 × 30cm, every cave one strain.
As can be seen from Figure 1: the mass of 1000 kernel repeating 1 is 1.49169;Repeat 2 thousand grain weigths maximum, be 1.64589;Weight Secondly multiple 3 thousand grain weigths, are 1.55769;Repeat 4 thousand grain weigths minimum, be 1.36779.The mass of 1000 kernel meansigma methods repeated Being 1.51579, repeat to there is most flat seed in 4 seeds, being likely to be due to salvia seeds in this repeats has more jejune cause , it is seen that conventional Radix Salviae Miltiorrhizae kind mass of 1000 kernel is about about 1.69.
Above step 1)-3) the excellent genic process of cultivation that is substantially carried out, in the process, roguing is Avoid plantation, results, storage occur the measure of mechanical admixture.Seedling Stage in medicinal plants will differ from the miscellaneous of breeding Plant and pull out, retain the pure strain meeting breeding feature, characteristic.It is further noted that arrangement crop rotation, it is to avoid different cultivars of the same race is front and back Stubble, in order to avoid making after anything left from previous crops plant to mix.
In order to keep good strains of seeds, artificially breeding is possible not only to remove hybrid strain, and has and consolidate and improve good characteristic Effect.Depending on the method for selection-breeding and standard are the feature according to medicinal plants, characteristic and effective ingredient, the method for selection-breeding has individual plant Selection-breeding, mixing selection-breeding and improvement mixing selection-breeding.Cultivate new varieties and stock breeding and all should use this selection, but they Purpose is different, and what the former selected is intended to exceed original kind, reaches to cultivate the purpose of new varieties;The latter is purification and rejuvenation, mesh Be retain original kind Optimality.
Second, below for simulation wild Radix Salviae Miltiorrhizae seedling breeding environment, breed intimate wild Radix Salviae Miltiorrhizae seedling.
4) foundation simulation wild Radix Salviae Miltiorrhizae seedling breeding base is used: simulation wild Radix Salviae Miltiorrhizae seedling breeding base selects the most partly Without appointing river polluter in the 5km of footpath, air quality meets GB3095-1996 first quality standard, and height above sea level is 250m 400m, Natural vegetation 40%-60%, the gradient is 10-20 degree, and nursery stage precipitation mid-July mid-June should account for 20% left side of annual precipitation The right side, temperature is more than 30 DEG C, and light application time is more than 9 hours, and shading rate≤65-70% in after planting 11 days, irrigation water meets GB5084-92 first quality standard, soil, with sandy red, yellow soil, meets GB15618-1995 quality standard.
Different temperature has different impacts to the sprouting of salvia seeds.Salvia seeds germination rate under field conditions (factors) Low, the most only 30%-40%, and also it is uneven to emerge.Salvia seeds is germinating energy and germination percentage such as following table under condition of different temperatures 2, salvia seeds is all with optimal between 20 DEG C-30 DEG C, wherein the highest, at 20 DEG C, 25 DEG C and 30 DEG C of bars to repeat H percentage of seedgermination 67%, 71% and 71.3% is respectively reached under part;Repeat 3 percentages of seedgermination to take second place, under the same terms, be respectively 58.7%, 65% and 65%。
Table 2 different temperatures salvia seeds germination percentage
Germination percentage is also had an impact by different seedbeds i.e. soil, it can be seen that difference repeats Radix Salviae Miltiorrhizae kind from table 3 below Son germinating energy under the conditions of sandy loam and clay is relatively low, and germination required time is longer, owing to salvia seeds is little, and contained nutrient substance Few, plumule top soil ability is weak, and sandy loam and clay condition can provide again part nutritional labeling simultaneously, is conducive to improving seed and sends out Bud rate.Examined by above-mentioned experiment it follows that salvia seeds germination percentage and germinating energy under the conditions of sandy loam are above Under the conditions of clay, this also confirms in terms of one, the edaphic condition in the particularly genuine medicinal materials place of production, the main producing region of Radix Salviae Miltiorrhizae, typically All it mostly is the sandy loam on mountain area or hills.
Table 3 different seedbeds salvia seeds germination percentage
The impact on abundant Radix Salviae Miltiorrhizae yield traits of the differing texture soil, as shown in Table 4, Salvia miltiorrhiza Growth is sent out by different soils quality Educate and affect significant difference.Single plant yield sand relatively loam, clay are respectively increased 14.51 %, 37.31 %;Individual plant root bar number, clay Bar number is most, and sand is minimum, and clay reaches significant level with sand root bar number difference.This shows, sand is conducive to Radix Salviae Miltiorrhizae to produce The raising of amount, this is probably due to Radix Salviae Miltiorrhizae is phreatophyte, well developed root system, and layer depth sand thick, open-textured in native land is conducive to Root growth, poor because of sand water conservation fertilizer-preserving ability but then, may have influence on the number of root bar number, but with loam difference The most notable.
The impact on abundant Radix Salviae Miltiorrhizae yield traits of the table 4 different soils quality
The impact on abundant effective component in red sage content of the differing texture soil, table 5 shows, sand effective component in red sage content is Height, salvianolic acid B, tanshinone IIA content respectively reach respectively 4.36 %, 0.24 % of 8.87 %, 0.30 %, loam and clay With 4.85 %, 0.28 %.
Table 5 different soils quality active constituent content
5) use with fertilizer: before whole ground, use the health fertilizer 112.5kg-450kg that becomes thoroughly decomposed, uniformly for every 1 hectare wholely Spread fertilizer over the fields on the ground;Preferably plough shallowly, within tilth is 20cm, then in simulation wild Radix Salviae Miltiorrhizae simulation wild Radix Salviae Miltiorrhizae seedling breeding base Plough plain grinding thin;Simulation wild Radix Salviae Miltiorrhizae seedling breeding base 300cm plays high furrow, and high furrow clear span is 270cm, furrow width 30cm, Then the thin leveling of rake.
The different fertilities impact on Index of The Healthy Seedlings, affects including on the change of Radix Salviae Miltiorrhizae Seedling fresh weight, dry weight, to Radix Salviae Miltiorrhizae plant height, The impact of stem thickness, on Radix Salviae Miltiorrhizae shoot root bar number, the impact of fibrous root number, specific as follows:
Test sets Different Fertilization amount respectively (with (F1) 112.5 kg/hm2, (F2) 225 kg/hm2, (F3) 450 kg/ Hm2 represents), nursery on the same day on July 12, each factor three process, three design iterations of each process.
Prior to seeding, being all made into length 2.2 m, the wide 0.6 little furrow of m, before broadcasting sowing, every furrow engrave flat rake carefully, after broadcasting sowing at the bottom of flat rake Concora crush one time, rear earthing about 0.5cm, the most about 0.5m horizontal coverage shading net, uniformly sprays above and irrigates nursery water, hide Shady 30 days.Once, the most every furrow sample 6 strains, and every Index of The Healthy Seedlings is surveyed in totally 18 strains, and numerical value is averaged in sampling in every 10 days.
The dynamic changes of fresh weight of Radix Salviae Miltiorrhizae Seedling under different fertilities, figure it is seen that after JIUYUE 7, Different Fertilization amount The fresh weight differences of three process start to widen, once in 9 months one the last ten-days period F2 process also greater than F3 processs, F3 process is whole During seedling growth, fresh weight accounts for one and has superiority, and reaches maximum in mid or late October, and F1 processes and also exceedes in mid-October F2 process.
Observing from Fig. 3, compare with Fig. 2, have two contrary broken line variation tendencies, one is that fresh weight is at mid or late September F2 Reason, more than F3 process, can dry weight compare contrary, and another is that fresh weight Fl process has exceeded F2 process after mid-October, can It is that F2 processes always more than F1 process that dry weight compares.Can draw from these 2, big dose processed in mid or late September to October The first tenday period of a month, dry weight and fresh weight of plant seedlings was bigger, and this stage illustrates that the synthesis of thousand materials and accumulation need more inorganic elements, fertilising to contribute to doing more Heavily increasing, why the dry weight of another F2 can catch up with the dry weight of F3, and this is also in that F2 at its fertility level of later stage is just processing Its plant fresh weight that supports of good coupling is constituted, and dry weight and fresh weight of plant seedlings is bigger, excessive due to later stage fresh weight unlike F3, and fertility is the most too late F2 process, dry weight and fresh weight of plant seedlings is than on the contrary less than F2, so can catch up with.Also having the most a bit, why F2 processes in 9 months last ten-days period to October The middle ten days and the last ten days, fresh weight declined, and dry weight is rising the most always, and this turns to storage transhipment to be main with explained above from extroversion growth Reason is identical.
The dynamic change of Radix Salviae Miltiorrhizae Seedling plant height under different fertilities, knowable to Fig. 4 observation analysis, Different Fertilization amount processes Seedling Final plant height does not has anything significantly to affect, and three line weave ins all the time remove error of sampling, cannot analyse what rule Rule, it is seen that the plant height of Radix Salviae Miltiorrhizae nursery is not had anything to affect by fertilizer treatment.
From Fig. 5, observe the thick Trendline of stem, in addition to about mid-September, F2 processes more than F3 process, each period later Being substantially on F3 residence, F2 is placed in the middle, under F1 occupies, but obvious, the stem rough error that F2 and F3 processes is little away from all the time, here it is three Individual different fertilities processes the action rule of change thick to stem.
Under different fertilities, the dynamic change of Radix Salviae Miltiorrhizae shoot root bar number, shows in Fig. 6, and the root bar number of three different fertilities process has The most regular, on generally F2 residence, F3 is placed in the middle, and under F1 occupies, JIUYUE early and middle ten days F2 root bar number starts to surpass in reverse at F3 after bottoming out Reason, holds a safe lead later, and illustrates that high dose does not promote that root bar number increases.
Under different fertilities, the fibrous root number of Radix Salviae Miltiorrhizae Seedling dynamically changes, Fig. 7 show F2 process fibrous root number always stable increase it In, F3 processes and starts a decline after early September, and among a kind of change being in drastically of fibrous root number that F1 processes, from August The fibrous root that the fibrous root of No. 30 is up to JIUYUE 9 is minimum, arrives again the most of JIUYUE 19, then arrives bottoming out of JIUYUE 29, it is seen that palpus The change of radical amount is big.F3 processes and begins to decline after early September be owing to this process fertility is abundant, resistance _ and only Increasing fatting up and the absorption of mineral element of fibrous root, and F1 process fibrous root number drastically change it is understood that F1 process Seedling is constantly among a kind of inharmonic growth relationship, owing to, in its growth conditions, only a Fertilization Level condition is short of Outward, other raw elongate member are the most superior, so when overground part fast-growth, rapidly promoting the increasing of underground part fibrous root amount Many, to draw nutrient, such principal contradiction goes underground, and waits underground part situation to convert, and the growth of overground part is own is neglected, then Growth contradiction proceeds on the ground, and the raw length in underground is absorbed in again stagnation and even falls back, and, the most quickly but also turns round and look at a little in such a like this Head does not turns round and look at high fast-growing one under life one long status of foot and grows, and the drastically change of fibrous root number above just occurs.
Under different fertilities, the seedling vigorous index of Radix Salviae Miltiorrhizae Seedling dynamically changes, except from JIUYUE early and middle ten days to mid or late September in terms of Fig. 8 Seedling vigorous index F2 oneself more than F3 outside, the gap of other periods F2 and F3 is the most little, simply slightly lags behind the highest dose level, red Join as a kind of medicinal plants using root as results object, so one of critically important purpose of strong seedling cultivation cultivates root of hair exactly Many, in the strong sprout of well developed root system, in conjunction with root bar number above and the comparison of fibrous root number, F2 processes all has best advantage, the most all Factor, it can be deduced that F2 treatment effect is optimum, is suitable for nurturing staff.
6) sowing: the sowing time selects be the Radix Salviae Miltiorrhizae optimal sowing time in July mid-June, and every 667 application rates are 2.5kg, before type of seeding sowing, adds simulation wild Radix Salviae Miltiorrhizae seedling breeding base fine earth 7.5kg and mixes in every 2.5kg salvia seeds Even, it is sprinkling upon uniformly in the soil on high furrow, then rolls with the waddy of a diameter 20cm and suppress, it is also possible to Hsinchu besom Pat the seed spreading in soil, make salvia seeds be fully contacted with soil.
The different seeding quantity impact on Index of The Healthy Seedlings, the mainly impact on Radix Salviae Miltiorrhizae Seedling fresh weight, the impact of dry weight, plant height and stem The impact of thickness, shoot root bar number and the impact of Seedling yarn number.Test sets three different seeding quantities respectively (with (S1) 30 kg/hm2、 (S2) 60 kg/hm2、(S3) 90 kg/hm2, represent), nursery on the same day on July 12, three design iterations of each process.? Before sowing, being all made into length 2.2 m, the wide 0.6 little furrow of m, before broadcasting sowing, every furrow engrave flat rake carefully, concora crush one time at the bottom of flat rake after broadcasting sowing, after Earthing about 0.5cm, the most about 0.5m horizontal coverage shading net, uniformly sprays above and irrigates nursery water, shade 30 days.Every 10 Once, the most every furrow sample 6 strains, and every Index of The Healthy Seedlings is surveyed in totally 18 strains, and numerical value is averaged in it sampling.
Radix Salviae Miltiorrhizae Seedling dynamic changes of fresh weight under different seeding quantity, from fig. 9, it can be seen that after JIUYUE 7, three differences are broadcast The fresh weight that kind of amount processes starts bigger differentiation occur, and S1 processes and starts to steeply rise with S2 process, subsequently S2 enter again one slow Slow decline process.And S3 has a bigger decline process, increase process slowly subsequently into one.The decline process of S3 can Being considered, when Seedling enters fast growing period, the density processed due to S3 is excessive, and the water of striving between Seedling is striven fertilizer to strive space serious, Cause principal contradiction to become the mutual survival of the fittest, cause poor growth, mutual negative influence, bottom a few fore-telling sheet senilism, fresh dry Heavy simultaneously decline even falls back.After the competition of this vigorous growth decided the winner, sap one's vitality, so part of winning also is The increase phase entering fresh weight that slow J is unrestrained.
Under different seeding quantity, the dynamic change of Radix Salviae Miltiorrhizae Seedling dry weight, compares from Figure 10 with Fig. 9, except S2 arrived in mid or late September October early and middle ten days dry weight Trendline contrary with fresh weight Trendline outside, also S3 process arrive early November in mid or late October Dry weight and fresh weight of plant seedlings Trendline is contrary, shows as the export-oriented growth of now Seedling and turns to and stock based on nutritional labeling, some old and feeble yellows Lower blade nutrient substance accelerate to Seedling interior shifting, accelerate its aging death, thus plant fresh weight decline, the opposing party Face, although fresh weight declines, but dry composition is mostly transported storage, so dry weight adds on the contrary.
The dynamic change of Radix Salviae Miltiorrhizae Seedling plant height under different seeding quantity, from the plant height broken line graph of Figure 11 it can be seen that three not Processing with the nursery of application rate, in terms of plant height, difference is little, and S1 processes the minimum being constantly in plant height in early stage, subsequently by In rich water and growing space factor so that S1 processes peak of having the advantage the most again, and the plant height advantage of S2 is the biggest In S3 process.
The dynamic change that under different seeding quantity, Radix Salviae Miltiorrhizae Seedling stem is thick, on the thick broken line graph of stem of Figure 12 can go out, three differences The nursery of application rate processes, and also little in stem thick aspect difference, on the most always S1 residence, S2 S3 placed in the middle stem is the most minimum, in 9 months Before the first tenday period of a month, three thick broken lines of stem processed are the most overlapping, along with S1 Yu S2 Acceleration of growth afterwards, the thick increment of stem substantially, and S3 Thick " the fall, it is seen that there is also huge in-fighting in struggle for existence, and struggle between plant, unexpectedly by waist thick for stem on the contrary of stem Also attenuate.Visible Radix Salviae Miltiorrhizae is during nurturing staff, and suitable application rate is most important.
The dynamic change of Radix Salviae Miltiorrhizae shoot root bar number under different seeding quantity, as can be seen from Figure 13, the root bar number that S3 processes is minimum, In about the 19 days October transplanted, substantially oneself reaches the level that S1 processes to the root bar number of S2, but Sl processes due to application rate Little, emergence rate is low in addition, and the commercial seedling amount of per mu yield is the least, loses the meaning of strong seedling cultivation.
Under different seeding quantity, Radix Salviae Miltiorrhizae Seedling fibrous root bar number dynamically changes, Figure 14 reflection draw, three different seeding quantity nursery In the dynamically change of fibrous root number very acutely, three process have super phenomenon mutually at different times, it is not difficult to find after careful observation, The fibrous root being actually S3 and s1 process dynamically changes acutely, and the dynamically change broken line then ratio that S2 processes is shallower, in fact This is to be processed the unreasonable group structure of nursery by S3 and S1 two to cause, and S3 processes the big promotion of early stage colony and sends out fibrous root, later stage Colony is big, hinders and sends out fibrous root, and S1 processes the little obstruction of early stage colony and sends out fibrous root, and fibrous root is sent out in the little promotion of later stage colony.For why S1 mid or late September to October early and middle ten days fibrous root number have one drastically to decline process, be perhaps on the one hand because some fibrous roots and send out Educating for bigger 2 grades of roots, be on the other hand because supplying 2 grades of roots, other fibrous roots are degenerated, furthermore be just because colony's knot that S1 processes Structure is too small, and along with mercury dropped is very fast, day and night temperature strengthens, and causes ground temperature to decline and accelerates, and fibrous root root of hair is slow, and new fibrous root generates Amount, not as good as the old and feeble amount of degradation of the existing system of fibrous root, causes total fibrous root bar number to accelerate on the contrary to reduce.
Under different seeding quantity, Radix Salviae Miltiorrhizae Seedling seedling vigorous index dynamically changes, from Figure 15, the variation diagram of seedling vigorous index it can be seen that After JIUYUE 7, seedling vigorous index starts drastically to break up, and S1 holds a safe lead, and S2 processes the most top, and S3 processes significantly to fall After in the above two.
The different sowing dates impact on Index of The Healthy Seedlings, mainly can be to seedling-growing time and regularity and the growth promoter of seedling Produce impact.Test sets three different sowing dates (representing with (Tl) June 30, (T2) July 12, (T3) July 29) respectively; 7.12 sowings, by each factor three process, three design iterations of each process.Prior to seeding, all it is made into long 2.2 m, wide by 0.6 The little furrow of m, before broadcasting sowing, every furrow engrave flat rake carefully, concora crush one time, rear earthing about 0.5cm, the most about 0.5m water at the bottom of flat rake after broadcasting sowing Flat coverage shading net, uniformly sprays above and irrigates nursery water, shade 30 days.Once, the most every furrow sample 6 in sampling in every 10 days Strain, every Index of The Healthy Seedlings is surveyed in totally 18 strains, and numerical value is averaged.
Different sowing dates is on abundant Radix Salviae Miltiorrhizae seedling-growing time and the impact of regularity, as shown in Table 20, when T1, T2, T3 process is emerged Between be all 7 d, relatively T4, T5, T6 process is emerged early, and the regularity emerged is high.After showing seed collection, nursery the most early, is imitated Fruit is the best.
The different seedling raise period of table 20 is emerged situation
Note: neat (emergence rate>=90 %), the most irregular (75%≤emergence rate < 90%=, irregular (emergence rate<75 %).
The different seedling raise period impacts on abundant Radix Salviae Miltiorrhizae seedling development, specific as follows:
(1) impact on plant height
Figure 16 shows, the Seedling that different seedling raise periods bring out, between plant height, difference reaches pole significance level, is wherein processed as with T2 The highest, reach 26.7 cm, remaining date of seeding Seedling plant height is on a declining curve with the postponement of sowing time, T1, T3 process respectively with T2 process Differing 4.5 % and 11.2 %, T4 process and differ 32.2 % with T2 process, T5, T6 process average plant height and are respectively 7.7 cm and 4.9 Cm, T5, T6 process be respectively T2 process 28.8 % and 18.4 %.
(2) impact of leaf area total on individual plant
Being found out by Figure 17, between T1, T2 process, difference does not reaches pole significance level, and all reaches extremely notable water between other date of seeding Flat, wherein T3 processes individual plant total leaf area maximum, and T1, T2 process and differ from it by 8.8 % and 6.9 % respectively.This may be with T1, T2 The part processing blade is withered relevant.T3 processes and has exceeded 36.2% than T4 process, and T5, T6 process is T3 process respectively 30.0 % and 13.6 %, this species diversity may be caused too late by the sowing time.
(3) impact on root growth
As shown in Table 23, different seedling raise period seedling maximum root diameters taper off trend, wherein T1, T2, T3 process with T4, Between T5, T6 process, difference reaches pole significant level, and between T4 and T6 processes, difference reaches pole significant level, without significance difference between other process Different.This shows, it is the most sturdy that T1, T2, T3 process seedlings root.
Maximum root length, T1 processes the longest, and and other date of seeding between difference reach pole significant level;T6 processed and between other date of seeding Having pole significant difference, T3 and T5 also reaches pole significant level between processing.
The total radical of individual plant, T2 processes at most, and T1 process is taken second place.Propping up radical is also that T2 processes at most, and T5, T6 process does not has Supporting root.Radical is many, is more beneficial for surviving of Radix Salviae Miltiorrhizae Seedling when transplanting, and haves laid a good foundation for obtaining higher yield.
The different seedling raise period root COMPARISON OF CHARACTERS of table 23
Note: significance test is Duncan duncan's new multiple range method, alphabetical identical person indicates without significant difference, letter difference person's table Show and reach 5 %(lower cases), 1 %(capitalization) significant level, lower with.
(4) impact on abundant Radix Salviae Miltiorrhizae Seedling Biomass
Different seedling raise periods are relatively big (table 24) on the impact of Radix Salviae Miltiorrhizae seedling overground part fresh weight, and T2 processes Seedling overground part fresh weight Greatly, T1 process is taken second place, other trend that tapers off, and T1, T2, T3 process and between T5, T6 process, difference reaches pole significant level, at other Electrodeless significant difference between reason;The Drying rate of overground part successively successively decreases successively by seedling raise period;Underground part fresh weight and root Drying rate become Change similar with fresh weight change on the ground.
Table 24 different seedling raise period Seedling Biomass change
(5) the dynamic change of different seedling raise period abundant Radix Salviae Miltiorrhizae seedling leaves growths
Figure 18 shows, seedling leaves growth substantially relatively T5, T6 that T1, T2, T3, T4 process process growth quickly, front four In individual date of seeding, the growth of seedling leaves presents the difference of growth speed with the priority of seedling raise period, the seedling leaves growth that T1 processes The fastest.There is a blade quick rise period at the growth of seedling initial stage to mid-August, increase in notch cuttype after mid-August;
As shown in figure 18, at the beginning of 9 months after T2 to process leaf growth speed slow, less than T3, T4 process, this may with T2 at The growth branch of reason underground part root is relevant, and the growth that T5, T6 process seedling leaves is the most delayed, it is seen that the elder generation of seedling raise period Growth to seedling overground part blade has large effect afterwards.
7) set up simulation wild Radix Salviae Miltiorrhizae and breed growing environment:
(1) play staking out: every 2.5m plays the little staking out of a Φ 4-7cm high for 35cm in the furrow of base, horizontal on furrow take One root length 3.1m, the bamboo stem of Φ 3cm-5cm, and by bamboo stem tightly tie up in little staking out, bamboo stem is away from ground 30cm;
(2) cover net: select shading rate 65-70%'s to herd with sunshade net, and will herd and ride over uniformly on bamboo stem with sunshade net, In staking out, shaded effects are affected with Anti-blow-open with sunshade net tying up to tightly by herding with rope.
The impact of Radix Salviae Miltiorrhizae seedling is analyzed by different seedling raising mannerses as follows:
On plant height, leaf area and the impact of root character
Table 26 shows, the seedling leaf area of Shading net covering and the longest root are thick, and all pole is significantly higher than Wheat-straw mulching;Plant height, Wheat-straw mulching pole is significantly higher than Shading net covering;The longest both roots are without difference.This shows that Shading net covering nursery is favourable In growth of seedling.
The different seedling raising manners of table 26 is on seedling aerial parts and the impact of root character
Impact on abundant Salvia miltiorrhiza Growth
Table 27 understands, and on the ground, underground fresh weight and dry weight, Shading net covering all pole is significantly higher than Wheat-straw mulching, and Drying rate is also Higher than Wheat-straw mulching.This shows that Shading net covering is more beneficial for the accumulation of abundant Radix Salviae Miltiorrhizae seedling dry.
The different seedling raising manners impact on abundant Radix Salviae Miltiorrhizae Seedling Biomass of table 27
Impact on the change of abundant Radix Salviae Miltiorrhizae seedling leaf Growth trends
As can be seen from Figure 19, Shading net covering nursery, seedling leaves growth is very fast, covers always above Wheat Straw.In 8 months Before ten days, two process seedling the most comparatively fast grows, and after late August, two process merit Seedlings are notch cuttype growth, and sunshade net seedling leaf Sheet growth rate is apparently higher than Wheat-straw mulching seedling, and this difference power that preferably may preserve soil moisture with sunshade net is relevant.
(3) water: water sprayed to uniformly above sunshade net with irrigation sprinkler, by the aperture of sunshade net, make water slowly Fall on nursery furrow;
(4) temperature and relative temperature in net: before emerging, net humidity is maintained at more than 25 DEG C, and relative humidity should be maintained at 75 Above, starting to emerge after 5 days, in 9 days, Radix Salviae Miltiorrhizae Seedling all sprouts substantially;
(5) take off net to put away sports equipment: Seedling goes out 5-7 days to take off net puts away sports equipment, and takes off net and puts away sports equipment and should carry out in the afternoon, should not be at point in the morning 10 Net is taken off, in case day drinks wound at 3 in afternoon;
Difference is shaded Radix Salviae Miltiorrhizae Seedling dynamic changes of fresh weight under natural law
Shown in Figure 20, three kinds of different natural law that shelter from heat or light represent with R1, R2, R3 respectively, represent 10 days, 30 days and 60 respectively My god, three differences natural law that shades processed fresh weight before mid or late September all in the quick rise period, because at about No. 7 gas of JIUYUE Temperature has been decreased obviously, and along with mercury dropped, on the one hand the photosynthesis of antiperspirant ten certainly will gradually weaken;On the other hand, the autumn is entered In season, dry weather, air humidity is more and more less, also will have influence on the holding of leaf water, so in terms of two, from storehouse Saying, photosynthate reduces, and says from source, and water loss is accelerated, so there will be fresh weight after mid or late September reaches peak value, first has One decline in various degree, then after gradually adapting to ecological environment in autumn, fresh weight enters again the phase of being slowly increased.
Difference Radix Salviae Miltiorrhizae Seedling dry weight under natural law of shading dynamically changes
Shown in Figure 21, three differences natural law that shades processed thousandweight before mid or late September all in the quick weightening finish phase, but was What Rl processes before and after October 19 relative to process, dry weight have one so close, analyze reason, examine, knot Close limit fresh weight figure, it is found that R2 late September to October early and middle ten days have obvious fresh weight to decline, dry weight goes up on the contrary The process risen, then Rl processes also is having a less fresh weight to decline at the beginning of mid or late October to 11 month, dry weight rises on the contrary Process, this explanation is within the two time period, and two process and all show as the export-oriented growth of now Seedling and turn to nutritional labeling It is main for stocking, and the nutrient substance of the lower blade of some old and feeble yellows accelerates to Seedling interior shifting, accelerates its aging death, adds Moisture evaporates, so plant fresh weight drastically declines, on the other hand, although fresh weight declines, but dry composition is mostly transported storage Conceal, so dry weight adds on the contrary.This is also from a side illustration, transplants, due to nutrient within the two time Matter is all got up by greatly storage, it is possible to defeat the resistance to adverse environment condition after transplanting, strengthens nutrient and energy Supply, transplanting survival rate is high, returns seedling stage short.
Difference shade natural law Seedling Height change
Figure 22 shows, R1 processes the most with the obvious advantage on plant height, higher than other two process.R2 is placed in the middle, under R3 occupies, but Comparing mutually, R2 Yu R1 gap is the least, has several times also in intersection location.
Shown in Figure 23, R1 processes stem is thick the most with the obvious advantage, higher than other two process.R2 is placed in the middle, under R3 occupies, but Comparing mutually, R2 Yu Rl gap is the least.
Difference is shaded the dynamic change of Radix Salviae Miltiorrhizae shoot root bar number under natural law
Shown in Figure 24, root bar number also can be commercial seedling training as the key factor on yield composition basis after transplanting in strong sprout One of important references index educated, 12 it can be seen that R2 process nursery root of hair bar number had a clear superiority in, in mid-September from the graph After, the quantity of the biggest 2 grades of roots is most, is the feature of best buy Seedling.It is the poorest that R1 processes, but R3 processes owing to shading Overlong time, is unfavorable for increasing of root bar number
The difference Radix Salviae Miltiorrhizae Seedling fibrous root number under natural law that shades dynamically changes
Shown in Figure 25, root system be plant to moisture and the major organs of mineral absorption, be again that the various metabolism such as plant are produced The organ that thing synthesizes, assimilates, converts, therefore the growing state of root and mobility directly affect plant individual growing state, Trophic level and yield level.R2 from lower Figure 14 it can be seen that after mid or late September, turn to nutritional labeling stock into In main growth course, having the rapid, high volume emergence period of a fibrous root, in early or mid October already close to R1 process, this illustrates R2 Before catching up with transplanting, produce the newborn fibrous root that a large amount of vigor is stronger, be especially suitable for transplanting.
The difference Radix Salviae Miltiorrhizae Seedling seedling vigorous index under natural law that shades dynamically changes
Shown in Figure 26, from seedling vigorous index broken line comparison diagram, can be seen that R2 processes and R1 process, except last November 8 had Outside bigger decline, the most relatively, it is not difficult to find after careful observation when R2 processes seedling vigorous index decline, be but root bar number and fibrous root Number a large amount of emergence periods, also seedling vigorous index is not to be the bigger the better, and compares content on comprehensive, and R2 processes except having more great Beyond 2 grades of roots, newborn vigor fibrous root also has a quick stage of development before transplanting, and seedling vigorous index is the most relatively Rl, illustrates that R2 processes with the obvious advantage, is suitable for nurturing staff.
8) field management: should pull out the various weeds in field after taking off net in time, must not use chemical herbicide, simulation open country Giving birth to Radix Salviae Miltiorrhizae seedling breeding base, early stage answers relative humidity higher, and soil is the most dry just should water in time, and the later stage should before Seedling goes out garden Suitably control water, when running into rainfall excess weather, the water in simulation wild Radix Salviae Miltiorrhizae seedling breeding base should be discharged in time, should anti-root-rot Sick generation.
Based on the above, in simulation wild Radix Salviae Miltiorrhizae sapling multiplication growing environment, nursery base furrow width 300cm, uses The sanitary cleaning bamboo stem of 320cm length and the little timber of 50cm length are support sunshade net support, sunshade net overhead 25-30cm, use Online sprinkling irrigation, makes nursery base relative humidity should be maintained at 55%-60%, within 5-7 days, emerges, after in 10 days, Seedling all sprouts, and in the afternoon 4 Sunshade net is removed after Dian.
Soil water content is on Radix Salviae Miltiorrhizae growth of seedling and the impact of Biomass, and pot experiment uses vinyon alms bowl (straight Footpath is 10 cm, high 20 cm of basin), every basin soil 210 kg.The Radix Salviae Miltiorrhizae sowing time is on October 10th, 2011, every basin after emerging Stay 1 strain Radix Salviae Miltiorrhizae Seedling.After 30 d of emerging, Seedling size is to carry out soil moisture process during 8-10 leaf 1 heart (November 17).Examination Testing and arrange 4 soil moistures process, respectively soil moisture content is maintained at maxmun field capacity (experiment soil after measured Maxmun field capacity be 2410%) 15%-20% (W1, severe drought), 35%-40% (W2, mild drought), 55%-60% (W3, mild drought), 75%-80% (W4, adequate water supply).Weighing method is used to carry out artificial moisture Control, Every afternoon, 18:00 carried out moisturizing, weighed with precision 011 g electronic scale.Test sets 4 repetitions.Soil moisture processes 60 d Rear end is tested, and carries out sampling analysis.
Along with the raising of soil water content, W1-W3 processes the plant height of plant, the number of blade, lateral bud number, leatherleaf length, plumage Leaf top lobule length and width, main root length, main root are slightly significantly significantly increased with side radical;Compared with W1 process, W3 process is planted Strain These parameters adds 0.74,1169,10.50,1.00,0.80,0.89,0.98,0.66,1.96 times respectively. But adequate water supply (W4 process) can substantially inhibit Radix Salviae Miltiorrhizae under ground portion growth promoter because of soil's being over wet, compared with W3 process, W4 processes main root length, main root is thick and side radical reduces 35.81%, 42.65%, 56.14% respectively.The above results shows, moisture Salvia miltiorrhiza Growth is grown by adverse circumstance (severe drought and excess moisture) bigger inhibitory action.
Soil water content is required higher by Radix Salviae Miltiorrhizae, and mild drought processes (W3) Radix Salviae Miltiorrhizae seedling leaf weight, root weight and gross weight The highest, Water Stress is then the raising that significantly limit Radix Salviae Miltiorrhizae each position Biomass.Radix Salviae Miltiorrhizae root Drying rate and root/hat ratio Being usually along with the raising of soil water content, in the trend being greatly reduced, this shows that soil moisture is to Radix Salviae Miltiorrhizae ground and ground The distribution of lower part Assimilate has obvious regulating and controlling effect.
As shown in table 36,37, soil moisture too much (W4) has serious pressing down to the growth of Radix Salviae Miltiorrhizae under ground portion and Biomass Making use, compared with W3 process, W4 processes overground part leaflet weight and only have dropped 15.60%, but under ground portion root weight, root are rolled over Dry rate and root/hat are to have dropped 65.16% respectively than then, 30.88%, 58.44%, and this shows that soil moisture too much will be led Cause the longest stem and leaf of Radix Salviae Miltiorrhizae and the longest root.And, test also finding, adequate water supply process Radix Salviae Miltiorrhizae root, because of excess moisture, is also led Cause its epiblem cracking and root bark color is turned white, reduce red rooted salvia exterior quality.
Table 36 soil moisture is to Radix Salviae Miltiorrhizae seedling overground part growth effect
Table 37 soil moisture is to Radix Salviae Miltiorrhizae seedling underground part growth effect

Claims (1)

1. the propagation method simulating wild Radix Salviae Miltiorrhizae seedling, it is characterised in that: comprise the following steps that
1) excellent wild Radix Salviae Miltiorrhizae kind is selected good strains in the field for seed and is educated: Radix Salviae Miltiorrhizae Proterozoic select blade profile, flower pattern, on the ground cane and underground rhizome color, The Radix Salviae Miltiorrhizae that plant growing form is consistent, selects to infect without pest and disease damage, tanshinone IIA content >=0.4%, content of danshinolic acid B >=7% Wild good species strain, sets up simulation wild Radix Salviae Miltiorrhizae and fosters garden, carry out fostering observation;
2) the secondary individual plant selection of wild good species strain: in the simulation wild Radix Salviae Miltiorrhizae set up fosters garden, selects to meet open country raw The feature of Radix Salviae Miltiorrhizae kind, characteristic, kind strain be healthy and strong, best in quality, fine individual plant without pest and disease damage, tanshinone IIA content >= 0.4%, under the conditions of content of danshinolic acid B >=7%, carry out secondary individual plant selection, again selected individual plant is migrated to plant garden and fosters Garden is fostered;
3) foster garden to foster: to carry out the fine individual plant in fostering garden classifying, transplanting, carry out grain weight feature and differentiate, right Single Tibetan received by the good salvia plant seed list preferably gone out, and numbers in order, and as the material of selection-breeding new varieties, enters and fosters garden to comfort Educate;
4) simulation wild Radix Salviae Miltiorrhizae seedling breeding base is set up: in simulation wild Radix Salviae Miltiorrhizae seedling breeding base selection radius 5km around Without any polluter, air quality meets GB3095-1996 first quality standard, and height above sea level is 250m 400m, naturally plants By 40%-60%, the gradient is 10-20 degree, and nursery stage precipitation mid-July mid-June should account for about the 20% of annual precipitation, temperature Degree is more than 30 DEG C, and light application time is more than 9 hours, shading rate≤65% in after planting 11 days, and irrigation water meets GB5084- 92 first quality standards, soil is sandy red, yellow soil, meets GB15618-1995 quality standard;
5) use with fertilizer: use the health fertilizer 112.5kg-450kg that becomes thoroughly decomposed for every 1 hectare before whole ground, spread uniformly wholely Execute on the ground;Preferably ploughing shallowly in simulation wild Radix Salviae Miltiorrhizae seedling breeding base, within tilth is 20cm, then ploughs plain grinding thin;Simulation open country The raw every 300cm in Radix Salviae Miltiorrhizae seedling breeding base plays high furrow, and high furrow clear span is 270cm, furrow width 30cm, then the thin leveling of rake;
6) sowing: the sowing time selects early July mid-June to be the Radix Salviae Miltiorrhizae sowing time, and every 667 application rates are 2.5kg, broadcast Before the sowing of the method for kind, add simulation wild Radix Salviae Miltiorrhizae seedling breeding base fine earth 7.5kg in every 2.5kg salvia seeds and mix even, uniformly The soil being sprinkling upon on high furrow in, then roll with the waddy of a diameter 20cm and suppress, or pat with Hsinchu besom and spread Seed in soil, makes salvia seeds be fully contacted with soil;
7) set up simulation wild Radix Salviae Miltiorrhizae and breed growing environment:
(1) play staking out: every 2.5m plays the little staking out of a Φ 4-7cm high for 35cm in the furrow of base, horizontal on furrow take one The bamboo stem of long 3.1m, Φ 3cm-5cm, and by bamboo stem tightly tie up in little staking out, bamboo stem is away from ground 30cm;
(2) cover net: select shading rate 65-70%'s to herd with sunshade net, and will herd and ride over uniformly on bamboo stem with sunshade net, with rope In staking out, shaded effects are affected with Anti-blow-open with sunshade net tying up to tightly by herding;
(3) water: water sprayed to uniformly above sunshade net with irrigation sprinkler, by the aperture of sunshade net, make water slowly fall Enter on nursery furrow;
(4) temperature and relative humidity in net: before emerging net in temperature be maintained at more than 25 DEG C, relative humidity should be maintained at 75% with On, start to emerge after 5 days, in 9 days, Radix Salviae Miltiorrhizae Seedling all sprouts substantially;
(5) take off net to put away sports equipment: Seedling goes out 5-7 days to take off net puts away sports equipment, and takes off net and puts away sports equipment and should carry out in the afternoon, should not at 10 in the morning under Noon 3 takes off net, in case day drinks wound;
8) field management: the various weeds in field should be pulled out after taking off net in time, chemical herbicide must not be used, simulate wild pellet Joining seedling breeding base, early stage answers relative humidity higher, and soil is the most dry just should water in time, and the later stage should be suitable before Seedling goes out garden Control water, when running into rainfall excess weather, should discharge the water in simulation wild Radix Salviae Miltiorrhizae seedling breeding base in time, should anti-root rot Occur.
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CN107396744A (en) * 2017-09-22 2017-11-28 岷县中药材生产技术指导站 A kind of Radix Codonopsis seedling-raising technique
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