CN109479652A - A kind of simple and efficient polygonatum kingianurn seed seedling-raising method - Google Patents

A kind of simple and efficient polygonatum kingianurn seed seedling-raising method Download PDF

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Publication number
CN109479652A
CN109479652A CN201811272196.4A CN201811272196A CN109479652A CN 109479652 A CN109479652 A CN 109479652A CN 201811272196 A CN201811272196 A CN 201811272196A CN 109479652 A CN109479652 A CN 109479652A
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China
Prior art keywords
seed
culturing seedlings
polygonatum kingianurn
soil
seedling
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CN201811272196.4A
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Chinese (zh)
Inventor
孟珍贵
杨彦宇
陈晓虹
白跃进
杨生超
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Yunnan Agricultural University
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Yunnan Agricultural University
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Priority to CN201811272196.4A priority Critical patent/CN109479652A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi

Abstract

The present invention relates to seed seedling-raising technical fields, more particularly to a kind of simple and efficient polygonatum kingianurn seed seedling-raising method, i.e., sand storage link is not necessarily to after seed collection, using with adopting with the method broadcast, in ordinary greenhouse or greenhouse, sandy soil and fertile soil are uniformly mixed in the ratio of 1:2 and are used as seedling medium;After the polygonatum kingianurn fruit of harvesting is rubbed open, it is seeded in prepared matrix immediately;Dry and soft leaf is after planting covered a thin sheet of, budding can be completed once to mid-April next year in weeding of watering weekly.Not only emergence rate is up to 90% or more for the seed sowed with the method for the present invention, but also root growth and development is excellent.

Description

A kind of simple and efficient polygonatum kingianurn seed seedling-raising method
Technical field
The present invention relates to seed seedling-raising technical fields, and in particular to a kind of simple and efficient polygonatum kingianurn seed seedling-raising method.
Background technique
Rhizoma polygonati Polygonati Rhizoma takes justice in " essence of female soil ", and integration of drinking and medicinal herbs is a variety of plants of Liliaceae Polygonatum Object rhizoma polygonati Polygonatum sibiricum Red., polygonatum kingianurn P.kingianum Coll.et Hemsl. and polygonatum cyrtonema P. The general name of cyrtonema Hua3 kind plant roots and stems, Medicinal source is more extensive, nearly there is more than ten kind.Rhizoma polygonati is sweet in flavor, It is mild-natured, returns spleen, lung, kidney channel.In China, 2 000 years are exceeded to the medicinal history of rhizoma polygonati, has been one of Chinese medicine common drug.At me State's medicine allusion quotation, which writes in " angle's sesame grass warp " and Compendium of Material Medica, load: " rhizoma polygonati regulating and tonifying qi keeps the five internal organs tune good, muscle sufficiency, bone Marrow is strong, and not old for many years, with Bai Genghei, dedentition is more raw ".To rhizoma polygonati chemical component studies have shown that rhizoma polygonati contain sibiricoside, The Multiple components such as niacin, carbohydrate, quinones, amino acid and microelement.
Polygonatum kingianurn is commonly called as " big rhizoma polygonati ", also known as " successively high, celestial being's meal ", compared with other two kinds of rhizoma polygonatis, has growth speed The features such as degree is fast, and biomass accumulation is more, rhizomes is coarse and advantage.
The polygonatum kingianurn of Yunnan Province is distributed mainly on the ground such as Dali of Yunnan, Lijing, enlightening celebrating, Nujiang, though wild distribution is wide, Wild resource is abundant, but coyoting phenomenon is obvious, and stock number is reduced year by year;Simultaneously with continuous to the temperature of rhizoma polygonati exploitation both at home and abroad Heating especially in terms of epidemiology prevention and nutrition and health care, and young and middle-aged weight-reducing but does not subtract constitution etc., right Rhizoma polygonati demand is increasing.
Rhizoma polygonati wild resource shortage, introducing and planting, but seedling wretched insufficiency.Rhizoma polygonati modes of reproduction has to be carried out with seed Sexual propagation and carried out with rhizome two kinds of vegetative propagation, in the past to excavate based on wild Propagation of Rhizomes, can be provided Seedling quantity is very limited, seriously constrains the large area plantation of polygonatum kingianurn.
Because the tissue culture technique of polygonatum kingianurn is also immature, therefore seed seedling-raising is having for current solution rhizoma polygonati seedling shortage Effect approach.There are the raising technology of rhizoma polygonati and polygonatum cyrtonema seed, seed broken dormancy technology and plumule emergence etc. in the country more at present Report, but sprout for polygonatum kingianurn seed and be not much with the document of nursery.Year gold and jade, Nian Guifa et al. are to the resource point of polygonatum kingianurn Cloth and pseudo-wild cultivating are studied, and method for culturing seedlings used is that " seeds before seeding carries out lamination processing, i.e., will be freshly harvested Seed is put in people hole after mixing evenly in the ratio and damp sand of 1:3, and one layer of damp sand of top cover carries out vernalization, is broadcast to the 2nd year spring Kind." village is vertical et al. in polygonatum kingianurn seminal propagation, " seed for choosing mature and plump carries out the processing of sand hiding immediately, i.e., 1 part of seed, 3 parts of sand are uniformly mixed, and are stored in the hole of shady spot 30cm depth, keep wet." Dang Kang (2006) to rhizoma polygonati, polygonatum kingianurn, spend more Rhizoma polygonati seed carried out low-temperature stratification comparative test, and rhizoma polygonati seed sprouting as the result is shown is needed by " one room temperature of low temperature, one low temperature One room temperature " four-stage shortens the germination period, and polygonatum kingianurn and polygonatum cyrtonema then only need by the " low of a 60d or so The lamination process of a warm room temperature " breaks the suspend mode of its hypocotyl, and after being transferred to room temperature culture, epicotyl is not needed individually Low temperature latter stage of ripening, after hypocotyl development completely, epicotyl can form true leaf and be unearthed.Deng Yinglian (2011) from light, temperature, swash Element, low-temperature stratification many aspects handle rhizoma polygonati seed, and percentage of seedgermination is higher when temperature is 25 DEG C as the result is shown, are 57.5%, the gibberellin of 1000 mg/kg impregnates the seeding ratio preferably 81% of 2d.
In short, used seed treatment and method for culturing seedlings link is more, step is complicated, increase many fund costs, Management cost and time cost.It is studied according to me, whether seed has after-ripening to need to be inquired into.
Summary of the invention
For above situation, the present invention is tested by the seed treatment of distinct methods, finally sums up complete set technology, A kind of simple and efficient polygonatum kingianurn seed seedling-raising method is provided.
A kind of technical solution of the present invention: simple and efficient polygonatum kingianurn seed seedling-raising method, comprising:
Step 1, sandy soil and fertile soil are uniformly mixed in ordinary greenhouse or greenhouse, as seed seedling-raising matrix, by normal Rule method manages furrow, the high 10cm of furrow, the wide 1.2-1.5m of furrow;
Step 2, polygonatum kingianurn pericarp harvests after turning yellow after mid-September, with adopting with broadcasting, is not necessarily to sand storage, is seeded in step 1 system In standby seedling medium;
Step 3, fruit is rubbed open when sowing, using drilling method, seeding row spacing 5cm × 8cm, depth of planting about 2cm;
Step 4, dry and soft leaf is after planting covered a thin sheet of, is advisable with covering 3/4 native face, is then sprinkled profoundly water, water weekly later It once and in time weeds out the rank grass, until mid-April completes budding.
Further, in step 1, seedling medium in mass ratio, sandy soil: fertile soil=0.5-1.5:1.5-2.5.
Further, in step 1, seedling medium in mass ratio, sandy soil: fertile soil=1:2.
Further, in step 2, seed is handled with 80-150mg/L 6-BA.
Further, in step 2, seed is handled with 100mg/L 6-BA.
Further, 100 polygonatum kingianurn seeds are taken to be sowed by claim 1 the method, until mid-April sprouts Afterwards, 20 measurement bud ratios are sampled.
Further, significance analysis is carried out to result with SPSS19.0.
Further, seeding ratio is 91.7% or more.
Further, loam is used to replace sandy soil and fertile soil as seed culture matrix, seeding ratio 90%.
The present invention compared with prior art the utility model has the advantages that
(1) emergence rate is improved, that the polygonatum kingianurn seed that the present invention is handled is studied than before as a result, emergence rate is by 81% It is promoted below to 90% or more.
(2) it is not only sprouted using the seed that method of the invention is handled, but also root growth situation is good.
(3) method that the present invention records, it is easy to operate, it is at low cost, scale operation is carried out convenient for peasant.
Detailed description of the invention
Fig. 1 is germination situation (growing state of two kinds of matrix root systems) in greenhouse, and a is sandy soil-fertile soil matrix, b For loam matrix;
Fig. 2 is outdoor plantation germination (the seed root system growing state of the outdoor sowing in field).
Specific embodiment
Below with reference to the specific research embodiment of inventor, the present invention is further explained.It should be understood that these embodiments are only used In illustrating the present invention rather than limit the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, Those skilled in the art can various modifications may be made or change, such equivalent forms equally fall within right appended by the application to the present invention Claim limited range.
1 test method
1.1 polygonatum kingianurn seed sproutings processing
Mainly use 3 kinds of processing modes: 1. 4 DEG C deposition 60 days after the outdoor plantation in crop field (matrix is loam);2. with adopting With being sowed at greenhouse (matrix is sandy soil: fertile soil=1:2);3. with adopting with being sowed at greenhouse (matrix is loam).Every kind of processing point three Group repetition, 50 polygonatum kingianurn seeds of each repetition.Seed used in this experiment is plantation in the plant in 5 years or more on the farm agricultural university Hou Shan The ripening fruits of strain knot, is handled, the weeding of watering weekly directly sowed is primary respectively after in late September, picking fruit is rubbed open.
Mid-April 1.2 to next year, seed being dug out respectively and measures bud ratio, test result is shown with SPSS19.0 The analysis of work property.
2 results and analysis
2.1 everywhere get a haircut bud rate statistics
Each repeating groups to mid-April, every kind of processing dig out 20 seeds, count its germination percentage.Statistical result is shown in Table 1。
1 different disposal polygonatum kingianurn germination of table counts (unit: grain)
Will be obvious that from the data in table 1: with adopt with broadcast temperature it is indoor two groups, i.e., in sandy soil-fertile soil and loam Polygonatum kingianurn seeding ratio (90% or more) much higher than 4 DEG C deposition 60 days after the outdoor polygonatum kingianurn for being seeded in agricultural university farm in crop field Seed (average emergence rate be 51.7%) illustrates with adopting with the sprouting for broadcasting highly beneficial polygonatum kingianurn seed.
Polygonatum kingianurn seed is handled with various concentration 6-BA, the processed polygonatum kingianurn seed of 100mg/L6-BA goes out as the result is shown Seedling rate is apparently higher than 50,200,300mg/L6-BA treated sprouting situation.It can be seen that 100mg/L 6-BA sprouts polygonatum kingianurn seed Hair has stronger facilitation.
The statistics of 2.2 polygonatum kingianurn seed root growth situations and root long:
In the greenhouse using with the seed adopted with broadcasting method, not only germination percentage is high, but also root growth is good;And it is heavy through 4 DEG C The polygonatum kingianurn seed of outdoor plantation after product processing, not only germination percentage is low, and the growing state of root is also far away from first two groups.And with regard to greenhouse In for plant two groups, the root that sandy soil-fertile soil mixes in matrix is more excellent than the root growth in loam.By measuring them The length of root, root long data are shown in Table 2.
The statistics (unit: cm) of 2 different disposal polygonatum kingianurn seminal root length of table
Data can be seen that from table, the matrix that 1 part of sandy soil and 2 parts of fertile soil blend together, to the growth fraction of polygonatum kingianurn seminal root Simple loam is more advantageous.This and the gas permeability of soil, water-retaining property, nutrient etc. are related.
Each 3 groups duplicate seeds of two kinds of processing in greenhouse are taken to sprout the picture (i.e. attached drawing 1) taken root and 4 DEG C of depositions after 60 days The outdoor plantation seed in crop field is sprouted the picture (i.e. attached drawing 2) taken root and is compared, it is evident that: 1. polygonatum kingianurn seed is in greenhouse In more outdoor plantation well-grown, it is especially best with matrix growth that 1 part of sandy soil and 2 parts of fertile soil blend together;2. Yunnan is yellow in greenhouse Smart seed has been sprouted more and root growth is good, and the seed root of hair of outdoor plantation is less and short.
In conclusion the optimum condition that polygonatum kingianurn seed is sprouted are as follows:
1. with adopting with being sowed at greenhouse and cover the emergence rate that the dry and soft leaf of a thin layer is greatly improved polygonatum kingianurn seed, and growing way Well;
2. the sprouting nursery that the mixed-matrix of sandy soil and fertile soil (1:2) are most suitable for polygonatum kingianurn seed.

Claims (10)

1. a kind of simple and efficient polygonatum kingianurn seed seedling-raising method characterized by comprising
Step 1, sandy soil and fertile soil are uniformly mixed in ordinary greenhouse or greenhouse, it is routinely square as seed seedling-raising matrix Legal principle furrow, the high 10cm of furrow, the wide 1.2-1.5m of furrow;
Step 2, polygonatum kingianurn pericarp harvests after turning yellow after mid-September, with adopting with broadcasting, is not necessarily to sand storage, is seeded in step 1 preparation In seedling medium;
Step 3, fruit is rubbed open when sowing, using drilling method, seeding row spacing 5cm × 8cm, depth of planting about 2cm;
Step 4, dry and soft leaf is after planting covered a thin sheet of, is advisable with covering 3/4 native face, is then sprinkled profoundly water, watering is primary weekly later And weed out the rank grass in time, until mid-April completes budding.
2. method for culturing seedlings according to claim 1, which is characterized in that in step 1, seedling medium in mass ratio, sandy soil: rotten Grow soil=0.5-1.5:1.5-2.5.
3. method for culturing seedlings according to claim 1, which is characterized in that in step 1, seedling medium in mass ratio, sandy soil: rotten Grow soil=1:2.
4. method for culturing seedlings according to claim 1, which is characterized in that in step 2, seed is at 80-150mg/L 6-BA Reason.
5. method for culturing seedlings according to claim 4, which is characterized in that in step 2, seed is handled with 100mg/L 6-BA.
6. method for culturing seedlings according to claim 1, which is characterized in that take 100 polygonatum kingianurn seeds by described in claim 1 Method is sowed, until sampling 20 measurement bud ratios after budding mid-April.
7. method for culturing seedlings according to claim 6, which is characterized in that carry out significance analysis to result with SPSS19.0.
8. method for culturing seedlings according to claim 1-7, which is characterized in that seeding ratio 91.7%.
9. according to claim 1, the described in any item method for culturing seedlings of 4-7, which is characterized in that replace sandy soil and humic using loam Soil is used as seed culture matrix.
10. method for culturing seedlings according to claim 9, which is characterized in that seeding ratio 90%.
CN201811272196.4A 2018-10-30 2018-10-30 A kind of simple and efficient polygonatum kingianurn seed seedling-raising method Pending CN109479652A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111316878A (en) * 2020-03-27 2020-06-23 罗平县经济作物技术推广站 Intensive field planting method for polygonatum kingianum
CN111955299A (en) * 2020-08-31 2020-11-20 丽水市林业技术推广总站 High-germination-rate drought-resistant polygonatum cyrtonema planting method
CN112205261A (en) * 2020-10-21 2021-01-12 丽江星宇生物开发有限公司 Method for improving germination rate and seedling rate in polygonatum kingianum breeding process

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103340070A (en) * 2013-06-09 2013-10-09 上高县勃发种植专业合作社 Standardized planting technology of polygonatum
CN104304017A (en) * 2014-10-17 2015-01-28 南京帝道农业科技有限公司 Intermediate propagation method of polygonatum punctatum tissue culture
CN105660219A (en) * 2016-01-20 2016-06-15 贵州大学 Polygonatum cyrtonema seed rapid emerging method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103340070A (en) * 2013-06-09 2013-10-09 上高县勃发种植专业合作社 Standardized planting technology of polygonatum
CN104304017A (en) * 2014-10-17 2015-01-28 南京帝道农业科技有限公司 Intermediate propagation method of polygonatum punctatum tissue culture
CN105660219A (en) * 2016-01-20 2016-06-15 贵州大学 Polygonatum cyrtonema seed rapid emerging method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111316878A (en) * 2020-03-27 2020-06-23 罗平县经济作物技术推广站 Intensive field planting method for polygonatum kingianum
CN111955299A (en) * 2020-08-31 2020-11-20 丽水市林业技术推广总站 High-germination-rate drought-resistant polygonatum cyrtonema planting method
CN112205261A (en) * 2020-10-21 2021-01-12 丽江星宇生物开发有限公司 Method for improving germination rate and seedling rate in polygonatum kingianum breeding process

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