CN112205261A - Method for improving germination rate and seedling rate in polygonatum kingianum breeding process - Google Patents
Method for improving germination rate and seedling rate in polygonatum kingianum breeding process Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a method for improving germination rate and seedling rate in a polygonatum kingianum breeding process, which comprises the following steps: s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 3-7 ℃; s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use. This method of improving germination rate and seedling rate among yunnan sealwort process of breeding, through selecting sandy loam, can increase the hydrophobicity of cultivating soil, prevent that moisture from piling up the decay that causes, rub the rape flower that withers through the setting to add the methanol liquid of 80% concentration, react with the stamen, make the inside brassinolide outflow of its stamen, with the gibberellin cooperation, adjust the seed of yunnan sealwort, awaken up the seed embryo, promote to sprout, thereby improve the germination rate, and reduce cost.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine planting, in particular to a method for improving germination rate and seedling rate in a polygonatum kingianum breeding process.
Background
Polygonatum kingianum is a Chinese medicinal material and is mainly distributed in Yunnan, Sichuan and Guizhou provinces in China. The sealwort is wild in mountain shrubs and forest edge grass in the shade, is cold-resistant, and seedlings can live through winter in the field but are not suitable for growing in dry areas. The germination time of the seeds is long, the germination rate is 60-70%, and the service life of the seeds is 2 years.
Because the polygonatum kingianum has high medicinal value and large market demand, artificial planting and cultivation become a trend. When the polygonatum kingianum is artificially planted and cultivated, the climate and the geology of places such as Yunnan and Sichuan are relatively accordant because the places are the original producing places of the polygonatum kingianum. In the seed propagation process, the germination rate and the seedling rate are high, and the overall seedling rate can reach more than 9.
In other areas in the south of China, such as the north of the Yangtze river to the south of the yellow river, the planting difficulty is high, the seedling rate is often lower than 85%, the seed germination rate is also lower than 80%, and the final survival rate of 100 seeds is lower than 7, so that the cost of the grower is increased.
Therefore, a method for improving the germination rate and the seedling rate in the polygonatum kingianum breeding process is provided.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for improving the germination rate and the seedling rate in the polygonatum kingianum breeding process.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving germination rate and seedling rate in a polygonatum kingianum breeding process comprises the following steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 3-7 ℃;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating a culture pit with the pit depth of 10cm, disinfecting the bottom of the foundation pit by adopting a disinfectant, and paving dry fine sand on the bottommost layer;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the ratio of 6:2:2, spreading to form a thickness of 1cm after mixing, and performing midday illumination for 2-3 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading with a kneader for 50-100min, collecting juice and flowers, and mixing with sandy soil added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil according to the proportion of 5:1, then putting the mixed soil and 2-3 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, and planting high-stem plants around the heat preservation film with the spacing larger than 50 cm.
Preferably, the cleaning and sterilizing in step S1 specifically includes: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying.
Preferably, the land selection condition of the step S3 of digging the cultivation pit is that the moisture content of the land is lower than 30% in the ventilated shady place.
Preferably, the disinfectant used for the bottom of the foundation pit in the step S3 is a diluted solution of chlorine dioxide disinfectant powder, and the concentration of the diluted solution is 1 mg/L.
Preferably, after the seeding in the step S6, watering is carried out every day, and the soil humidity is always kept to be not lower than 20%.
Preferably, the bottom main stem part of the high stem plant in the step S7 has at least 1m of branches and leaves.
The invention has the following beneficial effects:
1. the method for improving germination rate and seedling rate in polygonatum kingianum breeding process is different from conventional cultivation methods in that cold-stored polygonatum kingianum seeds are subjected to seed waking treatment, so that embryos in the polygonatum kingianum seeds in a long-term low-temperature environment slowly recover normal metabolism, then sunshine is carried out, when the seeds are wakened, time nodes where rape flowers are full are selected, the time for planting polygonatum kingianum is just suitable, and influence on germination of polygonatum kingianum caused by climate factors due to standard schedule planting is avoided.
2. According to the method for improving the germination rate and the seedling rate in the polygonatum kingianum breeding process, sandy loam is selected, the hydrophobicity of the cultivated soil can be increased, and the rotting caused by water accumulation is prevented.
Drawings
FIG. 1 is a schematic diagram of a cross-sectional structure of a cultivation pit in a real-time process of a method for improving germination rate and seedling rate in a polygonatum kingianum breeding process.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1
A method for improving germination rate and seedling rate in a polygonatum kingianum breeding process comprises the following specific steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 3 ℃; the cleaning and sterilizing process specifically comprises the following steps: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating cultivation pits with the depth of 10cm, disinfecting the bottom of the foundation pit by using a disinfectant, paving dry fine sand on the bottommost layer, and excavating the cultivation pits under the ground selection condition of ventilated shady places, wherein the water content of the ground is lower than 30%, and the disinfectant is a chlorine dioxide disinfectant powder diluent with the concentration of 1 mg/L;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the ratio of 6:2:2, spreading to form a thickness of 1cm after mixing, and performing midday illumination for 2 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading for 50min with a kneading machine, collecting juice and flowers, and mixing with sandy loam added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil in a ratio of 5:1, then putting the mixed soil and 2 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water; watering every day in the later period, and keeping the soil humidity not lower than 20% all the time;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering a film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, planting high-stem plants around the heat preservation film, keeping the distance between the high-stem plants to be more than 50cm, and enabling the main stem part at the bottom of the high-stem plants to be at least 1m without branches and leaves, as shown in figure 1 of the attached drawing of the specification.
Example 2
A method for improving germination rate and seedling rate in a polygonatum kingianum breeding process comprises the following specific steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 4 ℃; the cleaning and sterilizing process specifically comprises the following steps: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating cultivation pits with the depth of 10cm, disinfecting the bottom of the foundation pit by using a disinfectant, paving dry fine sand on the bottommost layer, and excavating the cultivation pits under the ground selection condition of ventilated shady places, wherein the water content of the ground is lower than 30%, and the disinfectant is a chlorine dioxide disinfectant powder diluent with the concentration of 1 mg/L;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the ratio of 6:2:2, spreading to form a thickness of 1cm after mixing, and performing midday illumination for 2 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading with a kneading machine for 60min, collecting juice and flowers, and mixing with sandy loam added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil in a ratio of 5:1, then putting the mixed soil and 2 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water; watering every day in the later period, and keeping the soil humidity not lower than 20% all the time;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering a film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, planting high-stem plants around the heat preservation film, keeping the distance between the high-stem plants to be more than 50cm, and enabling the main stem part at the bottom of the high-stem plants to be at least 1m without branches and leaves, as shown in figure 1 of the attached drawing of the specification.
Example 3
A method for improving germination rate and seedling rate in a polygonatum kingianum breeding process comprises the following specific steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 5 ℃; the cleaning and sterilizing process specifically comprises the following steps: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating cultivation pits with the depth of 10cm, disinfecting the bottom of the foundation pit by using a disinfectant, paving dry fine sand on the bottommost layer, and excavating the cultivation pits under the ground selection condition of ventilated shady places, wherein the water content of the ground is lower than 30%, and the disinfectant is a chlorine dioxide disinfectant powder diluent with the concentration of 1 mg/L;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the proportion of 6:2:2, spreading to form a thickness of 1cm after mixing, and carrying out lunch illumination for 3 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading for 70min with a kneading machine, collecting juice and flowers, and mixing with sandy loam added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil in a ratio of 5:1, then putting the mixed soil and 3 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water; watering every day in the later period, and keeping the soil humidity not lower than 20% all the time;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering a film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, planting high-stem plants around the heat preservation film, keeping the distance between the high-stem plants to be more than 50cm, and enabling the main stem part at the bottom of the high-stem plants to be at least 1m without branches and leaves, as shown in figure 1 of the attached drawing of the specification.
Example 4
A method for improving germination rate and seedling rate in a polygonatum kingianum breeding process comprises the following specific steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 6 ℃; the cleaning and sterilizing process specifically comprises the following steps: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating cultivation pits with the depth of 10cm, disinfecting the bottom of the foundation pit by using a disinfectant, paving dry fine sand on the bottommost layer, and excavating the cultivation pits under the ground selection condition of ventilated shady places, wherein the water content of the ground is lower than 30%, and the disinfectant is a chlorine dioxide disinfectant powder diluent with the concentration of 1 mg/L;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the proportion of 6:2:2, spreading to form a thickness of 1cm after mixing, and carrying out lunch illumination for 3 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading for 80min with a kneading machine, collecting juice and flowers, and mixing with sandy loam added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil in a ratio of 5:1, then putting the mixed soil and 3 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water; watering every day in the later period, and keeping the soil humidity not lower than 20% all the time;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering a film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, planting high-stem plants around the heat preservation film, keeping the distance between the high-stem plants to be more than 50cm, and enabling the main stem part at the bottom of the high-stem plants to be at least 1m without branches and leaves, as shown in figure 1 of the attached drawing of the specification.
The number of the dug pits in each of the above examples was 100, and the following table was obtained for the late germination and seedling data:
number of plants | Germination number in 10 days | Germination number of 15 days | Number of seedlings grown in 30 days | |
Example 1 | 200 | 160 | 185 | 180 |
Example 2 | 200 | 170 | 190 | 184 |
Example 3 | 300 | 260 | 289 | 285 |
Example 4 | 300 | 275 | 290 | 280 |
From the above statistical data, it can be known that, in the method provided by the present invention, the example 3 is the best example, the germination number of 15 days is 289, the corresponding germination rate is 96.3%, the number of line drawings after 30 days is 285, and the corresponding seedling rate is 98.6%, so that it can be seen that the best implementation mode is to irradiate the cultivation soil for 3 hours, and the kneading is the best 70min, so that brassinolide in rape pistil can be fully extracted, thereby providing germination regulation for polygonatum kingianum seeds, and increasing the germination rate.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A method for improving germination rate and seedling rate in a polygonatum kingianum breeding process is characterized by comprising the following steps:
s1, seed preservation: firstly, cleaning and sterilizing seeds of polygonatum kingianum, airing, mixing with wet sand containing 40% of water according to the ratio of 1: 4, storing at low temperature, and keeping the temperature at 3-7 ℃;
s2, waking up seeds: taking out the seeds of local rape after blooming, naturally thawing, removing fine sand, naturally drying, soaking in warm water at 10 deg.C for 30min, sun drying, soaking in warm water at 15 deg.C for 30min, taking out, air drying, and storing in shade for use;
s3, preprocessing: excavating a culture pit with the pit depth of 10cm, disinfecting the bottom of the foundation pit by adopting a disinfectant, and paving dry fine sand on the bottommost layer;
s4, preparing cultivation soil: selecting sandy loam, garden soil and humus soil, mixing according to the ratio of 6:2:2, spreading to form a thickness of 1cm after mixing, and performing midday illumination for 2-3 hours;
s5, preparing catalytic soil: selecting sandy loam and adding gibberellin diluent; collecting withered flowers of rape flowers, adding 80% methanol solution, kneading with a kneader for 50-100min, collecting juice and flowers, and mixing with sandy soil added with gibberellin diluent at a ratio of 8: 2;
s6, sowing: uniformly mixing the cultivation soil and the catalytic soil according to the proportion of 5:1, then putting the mixed soil and 2-3 polygonatum kingianum seeds into the cultivation pit pretreated by S3 together, and spraying a proper amount of clear water;
s7, seedling culture: when the outdoor temperature is lower than 15 ℃, covering film for accelerating seedling, covering a heat preservation film, removing the heat preservation film after seedling emergence, and planting high-stem plants around the heat preservation film with the spacing larger than 50 cm.
2. The method for improving germination rate and seedling rate in polygonatum kingianum breeding process according to claim 1, characterized in that: the cleaning and sterilizing in the step S1 specifically includes: heating to 100 deg.C with distilled water, cooling to 45 deg.C, soaking and cleaning seeds for 30 s, cleaning with clean distilled water cooled to 55 deg.C for 45 s, and naturally drying.
3. The method for improving germination rate and seedling rate in polygonatum kingianum breeding process according to claim 1, characterized in that: the land selection condition of the step S3 for digging the cultivation pit is that the moisture content of the land is lower than 30% at the ventilated shady place.
4. The method for improving germination rate and seedling rate in polygonatum kingianum breeding process according to claim 1, characterized in that: and in the step S3, the disinfectant used for the bottom of the foundation pit is chlorine dioxide disinfectant powder diluent, and the concentration of the diluent is 1 mg/L.
5. The method for improving germination rate and seedling rate in polygonatum kingianum breeding process according to claim 1, characterized in that: and (5) watering every day after sowing in the step S6, and keeping the soil humidity not lower than 20% all the time.
6. The method for improving germination rate and seedling rate in polygonatum kingianum breeding process according to claim 1, characterized in that: the bottom main stem part of the high stem plant in the step S7 has at least 1m of branches and leaves.
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